Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72034.t1 | GAU14248.1 | 84.7 | 170 | 24 | 1 | 1 | 170 | 1 | 168 | 3.10E-83 | 317.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72034.t1 | A0A392QMR0 | 83.7 | 172 | 24 | 2 | 1 | 170 | 1 | 170 | 5.6e-82 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72034 | MS.gene94983 | PPI |
| MS.gene64289 | MS.gene72034 | PPI |
| MS.gene72034 | MS.gene064976 | PPI |
| MS.gene72034 | MS.gene53620 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72034.t1 | MTR_3g109880 | 62.428 | 173 | 56 | 2 | 4 | 171 | 3 | 171 | 8.83e-72 | 214 |
| MS.gene72034.t1 | MTR_6g088445 | 45.732 | 164 | 79 | 2 | 8 | 170 | 9 | 163 | 1.59e-46 | 150 |
| MS.gene72034.t1 | MTR_6g088475 | 43.125 | 160 | 81 | 2 | 8 | 166 | 9 | 159 | 4.85e-41 | 136 |
| MS.gene72034.t1 | MTR_1g046660 | 37.423 | 163 | 96 | 3 | 3 | 164 | 2 | 159 | 1.88e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72034.t1 | AT4G32870 | 46.988 | 166 | 76 | 4 | 1 | 165 | 1 | 155 | 6.80e-49 | 155 |
| MS.gene72034.t1 | AT2G25770 | 36.970 | 165 | 94 | 4 | 1 | 164 | 1 | 156 | 2.38e-34 | 119 |
| MS.gene72034.t1 | AT2G25770 | 36.970 | 165 | 94 | 4 | 1 | 164 | 1 | 156 | 2.38e-34 | 119 |
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATACTTGTTATAAACTTGA+AGG | 0.368519 | 3.4:-95491437 | MS.gene72034:CDS |
| AGTTTATAACAAGTATCAAT+TGG | 0.379451 | 3.4:+95491443 | None:intergenic |
| GAAAGTACTGCCACTGCAAA+TGG | 0.400092 | 3.4:-95491231 | MS.gene72034:CDS |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.4:+95491283 | None:intergenic |
| TTGCCACACTATTTCCTCTT+CGG | 0.429431 | 3.4:+95491502 | None:intergenic |
| TATAAACTTGAAGGAATTGA+AGG | 0.434983 | 3.4:-95491428 | MS.gene72034:CDS |
| ATAGGACAAACAACGTTGTA+CGG | 0.455654 | 3.4:+95491301 | None:intergenic |
| AAAGCCACCGTTGAGGTTAG+AGG | 0.466959 | 3.4:-95491527 | MS.gene72034:CDS |
| GTTTATAACAAGTATCAATT+GGG | 0.483224 | 3.4:+95491444 | None:intergenic |
| CAAAATTTGAAATCTTACAT+TGG | 0.486428 | 3.4:-95491128 | MS.gene72034:CDS |
| TGCAAAGTCCACCCTTCCAC+AGG | 0.489996 | 3.4:+95491149 | None:intergenic |
| GAAGGAATTGAAGGCCAACC+TGG | 0.518392 | 3.4:-95491419 | MS.gene72034:CDS |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.4:-95491187 | MS.gene72034:CDS |
| CTTGTGATCCTGTGGAAGGG+TGG | 0.546087 | 3.4:-95491157 | MS.gene72034:CDS |
| TAGGACAAACAACGTTGTAC+GGG | 0.547037 | 3.4:+95491302 | None:intergenic |
| TGGAAACTTTCTCAATTGCA+TGG | 0.549994 | 3.4:-95491108 | MS.gene72034:CDS |
| TGTGCTTGTGATCCTGTGGA+AGG | 0.557196 | 3.4:-95491161 | MS.gene72034:CDS |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.4:-95491186 | MS.gene72034:CDS |
| CAATTGGGAGCCACTTGTGT+AGG | 0.573330 | 3.4:+95491459 | None:intergenic |
| TTCGGTTCCTCTAACCTCAA+CGG | 0.588456 | 3.4:+95491520 | None:intergenic |
| GAAATTGTTGATAACAACAT+GGG | 0.602430 | 3.4:-95491278 | MS.gene72034:CDS |
| GTGCTTGTGATCCTGTGGAA+GGG | 0.612275 | 3.4:-95491160 | MS.gene72034:CDS |
| TGAAATTGTTGATAACAACA+TGG | 0.627477 | 3.4:-95491279 | MS.gene72034:CDS |
| GGTGATGATGCTAAACGTGT+AGG | 0.644167 | 3.4:-95491203 | MS.gene72034:CDS |
| GTACTGCCACTGCAAATGGA+CGG | 0.644735 | 3.4:-95491227 | MS.gene72034:CDS |
| GCACAATAACGAATGAGACC+AGG | 0.650328 | 3.4:+95491401 | None:intergenic |
| GGAGTGTGCTTGTGATCCTG+TGG | 0.652171 | 3.4:-95491165 | MS.gene72034:CDS |
| TCACCACCGTCCATTTGCAG+TGG | 0.660619 | 3.4:+95491221 | None:intergenic |
| TGAGGTTAGAGGAACCGAAG+AGG | 0.671862 | 3.4:-95491516 | MS.gene72034:CDS |
| GGTTCCTCTAACCTCAACGG+TGG | 0.706209 | 3.4:+95491523 | None:intergenic |
| GGAAGGTAAAGCCACCGTTG+AGG | 0.711159 | 3.4:-95491534 | MS.gene72034:CDS |
| AATAACGAATGAGACCAGGT+TGG | 0.715813 | 3.4:+95491405 | None:intergenic |
| GAACCGAAGAGGAAATAGTG+TGG | 0.729750 | 3.4:-95491505 | MS.gene72034:CDS |
| CATGGGATTTAAGAACTACG+TGG | 0.741699 | 3.4:-95491261 | MS.gene72034:CDS |
| CTGCCACTGCAAATGGACGG+TGG | 0.775966 | 3.4:-95491224 | MS.gene72034:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTATAACAAGTATCAAT+TGG | + | chr3.4:95491190-95491209 | None:intergenic | 20.0% |
| !! | GTTTATAACAAGTATCAATT+GGG | + | chr3.4:95491189-95491208 | None:intergenic | 20.0% |
| !!! | CAAAATTTGAAATCTTACAT+TGG | - | chr3.4:95491502-95491521 | MS.gene72034:CDS | 20.0% |
| ! | AGGAAAATAAGCTAAAAAGA+TGG | - | chr3.4:95491074-95491093 | MS.gene72034:CDS | 25.0% |
| ! | GGAAAATAAGCTAAAAAGAT+GGG | - | chr3.4:95491075-95491094 | MS.gene72034:CDS | 25.0% |
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.4:95491350-95491369 | None:intergenic | 25.0% |
| ! | TATAAACTTGAAGGAATTGA+AGG | - | chr3.4:95491202-95491221 | MS.gene72034:CDS | 25.0% |
| !! | TCTTTGAAAAACTTAACAAC+TGG | + | chr3.4:95491289-95491308 | None:intergenic | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.4:95491352-95491371 | MS.gene72034:CDS | 25.0% |
| !!! | GATACTTGTTATAAACTTGA+AGG | - | chr3.4:95491193-95491212 | MS.gene72034:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.4:95491351-95491370 | MS.gene72034:CDS | 25.0% |
| AATAAGCTAAAAAGATGGGA+AGG | - | chr3.4:95491079-95491098 | MS.gene72034:CDS | 30.0% | |
| TATTGTGCTTCCACAAAAAA+AGG | - | chr3.4:95491244-95491263 | MS.gene72034:CDS | 30.0% | |
| ! | ATATGCAAGCTCAATGTTTT+TGG | + | chr3.4:95491548-95491567 | None:intergenic | 30.0% |
| ! | ATTTTTGTAACCTACACAAG+TGG | - | chr3.4:95491161-95491180 | MS.gene72034:CDS | 30.0% |
| ATAGGACAAACAACGTTGTA+CGG | + | chr3.4:95491332-95491351 | None:intergenic | 35.0% | |
| TGGAAACTTTCTCAATTGCA+TGG | - | chr3.4:95491522-95491541 | MS.gene72034:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.4:95491444-95491463 | MS.gene72034:CDS | 35.0% | |
| !!! | TTCATCAACGCCTTTTTTTG+TGG | + | chr3.4:95491257-95491276 | None:intergenic | 35.0% |
| AATAACGAATGAGACCAGGT+TGG | + | chr3.4:95491228-95491247 | None:intergenic | 40.0% | |
| CATGGGATTTAAGAACTACG+TGG | - | chr3.4:95491369-95491388 | MS.gene72034:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.4:95491443-95491462 | MS.gene72034:CDS | 40.0% | |
| TAGGACAAACAACGTTGTAC+GGG | + | chr3.4:95491331-95491350 | None:intergenic | 40.0% | |
| TTGCCACACTATTTCCTCTT+CGG | + | chr3.4:95491131-95491150 | None:intergenic | 40.0% | |
| GAACCGAAGAGGAAATAGTG+TGG | - | chr3.4:95491125-95491144 | MS.gene72034:CDS | 45.0% | |
| GCACAATAACGAATGAGACC+AGG | + | chr3.4:95491232-95491251 | None:intergenic | 45.0% | |
| TTCGGTTCCTCTAACCTCAA+CGG | + | chr3.4:95491113-95491132 | None:intergenic | 45.0% | |
| ! | GGTGATGATGCTAAACGTGT+AGG | - | chr3.4:95491427-95491446 | MS.gene72034:CDS | 45.0% |
| !! | GAAAGTACTGCCACTGCAAA+TGG | - | chr3.4:95491399-95491418 | MS.gene72034:CDS | 45.0% |
| AAAGCCACCGTTGAGGTTAG+AGG | - | chr3.4:95491103-95491122 | MS.gene72034:CDS | 50.0% | |
| CAATTGGGAGCCACTTGTGT+AGG | + | chr3.4:95491174-95491193 | None:intergenic | 50.0% | |
| GAAGGAATTGAAGGCCAACC+TGG | - | chr3.4:95491211-95491230 | MS.gene72034:CDS | 50.0% | |
| GTACTGCCACTGCAAATGGA+CGG | - | chr3.4:95491403-95491422 | MS.gene72034:CDS | 50.0% | |
| GTGCTTGTGATCCTGTGGAA+GGG | - | chr3.4:95491470-95491489 | MS.gene72034:CDS | 50.0% | |
| TGAGGTTAGAGGAACCGAAG+AGG | - | chr3.4:95491114-95491133 | MS.gene72034:CDS | 50.0% | |
| TGTGCTTGTGATCCTGTGGA+AGG | - | chr3.4:95491469-95491488 | MS.gene72034:CDS | 50.0% | |
| CTTGTGATCCTGTGGAAGGG+TGG | - | chr3.4:95491473-95491492 | MS.gene72034:CDS | 55.0% | |
| GGAAGGTAAAGCCACCGTTG+AGG | - | chr3.4:95491096-95491115 | MS.gene72034:CDS | 55.0% | |
| GGAGTGTGCTTGTGATCCTG+TGG | - | chr3.4:95491465-95491484 | MS.gene72034:CDS | 55.0% | |
| GGTTCCTCTAACCTCAACGG+TGG | + | chr3.4:95491110-95491129 | None:intergenic | 55.0% | |
| TCACCACCGTCCATTTGCAG+TGG | + | chr3.4:95491412-95491431 | None:intergenic | 55.0% | |
| TGCAAAGTCCACCCTTCCAC+AGG | + | chr3.4:95491484-95491503 | None:intergenic | 55.0% | |
| CTGCCACTGCAAATGGACGG+TGG | - | chr3.4:95491406-95491425 | MS.gene72034:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 95491067 | 95491585 | 95491067 | ID=MS.gene72034 |
| chr3.4 | mRNA | 95491067 | 95491585 | 95491067 | ID=MS.gene72034.t1;Parent=MS.gene72034 |
| chr3.4 | exon | 95491067 | 95491585 | 95491067 | ID=MS.gene72034.t1.exon1;Parent=MS.gene72034.t1 |
| chr3.4 | CDS | 95491067 | 95491585 | 95491067 | ID=cds.MS.gene72034.t1;Parent=MS.gene72034.t1 |
| Gene Sequence |
| Protein sequence |
