AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72182

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72182.t1 MTR_8g096950 97.173 283 7 1 1 283 1 282 0.0 562
MS.gene72182.t1 MTR_5g010620 74.113 282 58 2 1 282 1 267 7.14e-149 417
MS.gene72182.t1 MTR_5g010620 74.113 282 58 2 1 282 33 299 1.13e-148 418
MS.gene72182.t1 MTR_3g110015 88.235 85 9 1 1 84 1 85 1.69e-44 146
MS.gene72182.t1 MTR_3g109978 88.235 85 9 1 1 84 92 176 7.18e-44 147
MS.gene72182.t1 MTR_7g055620 61.194 134 13 2 1 134 166 260 2.82e-42 149
MS.gene72182.t1 MTR_0411s0050 83.929 56 9 0 88 143 15 70 5.57e-27 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72182.t1 AT4G20330 66.312 282 92 2 1 282 1 279 9.37e-124 355
MS.gene72182.t1 AT4G21010 58.363 281 109 5 1 280 1 274 2.81e-96 285

Find 63 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCGTCGTATTCTACTTT+TGG 0.214350 4.2:+6928985 None:intergenic
TCCACCTTAATGTTTGCTTT+GGG 0.248514 4.2:+6927984 None:intergenic
ATCCACCTTAATGTTTGCTT+TGG 0.267923 4.2:+6927983 None:intergenic
GCAGCCACCACCGGAGTATT+AGG 0.268237 4.2:+6930034 None:intergenic
CAGGAGTTGTTGTTTACTTT+AGG 0.277978 4.2:+6927727 None:intergenic
TGAAAGCTGCAGGGCAGATT+TGG 0.288630 4.2:-6928055 MS.gene72182:CDS
ATGTTTGCTTTGGGGTCATT+CGG 0.320738 4.2:+6927993 None:intergenic
CTCTTTGCAGTGTTGGTAGC+AGG 0.337273 4.2:+6927873 None:intergenic
CATGCTTCATTTATTTGCTC+TGG 0.343949 4.2:+6929060 None:intergenic
CAATGTGATGGAAGACTTGC+AGG 0.356725 4.2:-6928756 MS.gene72182:intron
ATTGGTCAGCTTGGTTCTCT+TGG 0.364216 4.2:+6927776 None:intergenic
CGCTTCATCTGAGCACCAAC+GGG 0.368774 4.2:+6929917 None:intergenic
CATTGGGGTAAGCATCCTTT+AGG 0.372124 4.2:+6928774 None:intergenic
CTTAGACTTGGACTTGGACT+TGG 0.379977 4.2:+6927812 None:intergenic
TGTTTGCTTTGGGGTCATTC+GGG 0.391698 4.2:+6927994 None:intergenic
TGCAAGTCTTCCATCACATT+GGG 0.401716 4.2:+6928758 None:intergenic
GCTGACCAATTCCCATCTTC+CGG 0.404549 4.2:-6927763 MS.gene72182:CDS
TGACCCCAAAGCAAACATTA+AGG 0.415737 4.2:-6927988 MS.gene72182:CDS
TTGTAGGCTTTGAAAGCTGC+AGG 0.415846 4.2:-6928065 MS.gene72182:intron
ATGACCTAAAGCAGCTGTTT+CGG 0.418120 4.2:-6927959 MS.gene72182:CDS
GAGAAGGATCTTCAAAAGAA+TGG 0.418846 4.2:-6927903 MS.gene72182:CDS
ATAGGCAAAGTATGGTCTTA+AGG 0.421161 4.2:-6928855 MS.gene72182:intron
AAGCTCCGGAAGATGGGAAT+TGG 0.433158 4.2:+6927758 None:intergenic
CTTGGACTTGGACTTGGACT+TGG 0.442430 4.2:+6927818 None:intergenic
TACTCCGAAACAGCTGCTTT+AGG 0.444378 4.2:+6927955 None:intergenic
AGCATTGCGTCGAGCAAAGT+TGG 0.452004 4.2:-6930067 MS.gene72182:CDS
AGTCCTCCTCTTTGCAGTGT+TGG 0.455410 4.2:+6927866 None:intergenic
GATTTCATTCTTCTTAGACT+TGG 0.459783 4.2:+6927800 None:intergenic
CCACCTTAATGTTTGCTTTG+GGG 0.471247 4.2:+6927985 None:intergenic
GCTGTCCAACTTTGATTCAC+AGG 0.488075 4.2:-6928030 MS.gene72182:CDS
AGGACTGCAGCACAAATTGA+TGG 0.492183 4.2:-6927849 MS.gene72182:CDS
GATGCATTTGCAGCCACCAC+CGG 0.494981 4.2:+6930025 None:intergenic
GCTGCAAATGCATCAGCAAA+TGG 0.504031 4.2:-6930016 MS.gene72182:CDS
AAGCATGCTATGTTGATATG+AGG 0.505702 4.2:-6929044 MS.gene72182:CDS
GTCGTCCTGTGAATCAAAGT+TGG 0.513690 4.2:+6928025 None:intergenic
ATTCTTCTTAGACTTGGACT+TGG 0.515148 4.2:+6927806 None:intergenic
CTGCAAGTCTTCCATCACAT+TGG 0.523283 4.2:+6928757 None:intergenic
CTCTGCAAGAGCAACTAGAT+AGG 0.523289 4.2:-6930129 MS.gene72182:CDS
AAGTAGAATACGACGGGCAA+CGG 0.531226 4.2:-6928981 MS.gene72182:CDS
CGGGCAACGGTTCTCTTACA+AGG 0.541030 4.2:-6928968 MS.gene72182:intron
AAGATGGGAATTGGTCAGCT+TGG 0.557060 4.2:+6927767 None:intergenic
TGTTATTGATCTATTGCTTG+AGG 0.558507 4.2:-6929894 MS.gene72182:intron
ACCCAAAAGTAGAATACGAC+GGG 0.561755 4.2:-6928987 MS.gene72182:CDS
GGATGCTTACCCCAATGTGA+TGG 0.574607 4.2:-6928768 MS.gene72182:CDS
AAAACCTAATACTCCGGTGG+TGG 0.582600 4.2:-6930038 MS.gene72182:CDS
TAGCAGAAAACCTAATACTC+CGG 0.589094 4.2:-6930044 MS.gene72182:CDS
ACGCTTCATCTGAGCACCAA+CGG 0.595575 4.2:+6929916 None:intergenic
AGCTTCTTCAACTTATACGT+AGG 0.600973 4.2:-6928823 MS.gene72182:CDS
TGATATGATTGATATAGAGA+AGG 0.617718 4.2:-6927919 MS.gene72182:CDS
CCCCAAAGCAAACATTAAGG+TGG 0.623815 4.2:-6927985 MS.gene72182:CDS
AATTCTCAAGTGACACTGAG+AGG 0.626331 4.2:-6929970 MS.gene72182:CDS
AGCATGCTATGTTGATATGA+GGG 0.636991 4.2:-6929043 MS.gene72182:CDS
TGTAGGCTTTGAAAGCTGCA+GGG 0.643501 4.2:-6928064 MS.gene72182:intron
CTACCAACACTGCAAAGAGG+AGG 0.656479 4.2:-6927869 MS.gene72182:CDS
CTGCTACCAACACTGCAAAG+AGG 0.665956 4.2:-6927872 MS.gene72182:CDS
GCTTCATCTGAGCACCAACG+GGG 0.666699 4.2:+6929918 None:intergenic
ACTTATACGTAGGTATCCAG+AGG 0.677982 4.2:-6928813 MS.gene72182:CDS
CTTATACGTAGGTATCCAGA+GGG 0.688413 4.2:-6928812 MS.gene72182:CDS
AACCCAAAAGTAGAATACGA+CGG 0.689064 4.2:-6928988 MS.gene72182:CDS
GCAAGTCTTCCATCACATTG+GGG 0.693489 4.2:+6928759 None:intergenic
CTTCATCTGAGCACCAACGG+GGG 0.696908 4.2:+6929919 None:intergenic
CAGAAAACCTAATACTCCGG+TGG 0.697637 4.2:-6930041 MS.gene72182:CDS
TCTATATCAATCATATCACG+AGG 0.742182 4.2:+6927924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACCTTAATTTATAATAA+TGG + chr4.2:6929428-6929447 None:intergenic 10.0%
!! AATAAACAAAACAAATGAAT+AGG + chr4.2:6929558-6929577 None:intergenic 15.0%
!! ATTGAAACTAATAATATGTT+TGG - chr4.2:6928220-6928239 MS.gene72182:intron 15.0%
!! TTAATTTATAATAATGGCAA+CGG + chr4.2:6929422-6929441 None:intergenic 15.0%
!!! TTGTTTGTTTTTTCATTTAT+TGG - chr4.2:6928035-6928054 MS.gene72182:CDS 15.0%
!! AATTAAGTTCTCAATATGTT+AGG + chr4.2:6928362-6928381 None:intergenic 20.0%
!! ACTTAAATAGATGACATAAA+GGG - chr4.2:6928440-6928459 MS.gene72182:intron 20.0%
!! ATAAATTAAGGTTAATACTG+AGG - chr4.2:6929433-6929452 MS.gene72182:intron 20.0%
!! ATTAAGTTCTCAATATGTTA+GGG + chr4.2:6928361-6928380 None:intergenic 20.0%
!! GTTGCCATTATTATAAATTA+AGG - chr4.2:6929421-6929440 MS.gene72182:intron 20.0%
!! TTAATGTTTGTCTTGAAATA+TGG - chr4.2:6929655-6929674 MS.gene72182:intron 20.0%
!!! AAGAAGATCAAATGTTTTAT+GGG - chr4.2:6929321-6929340 MS.gene72182:intron 20.0%
!!! GTTATTTGTTTTTTGTCTAT+AGG - chr4.2:6929000-6929019 MS.gene72182:CDS 20.0%
!!! TATTAGTTTCAATTTGCAAT+TGG + chr4.2:6928213-6928232 None:intergenic 20.0%
!!! TCATAATGATATCTTTTTGT+AGG - chr4.2:6929792-6929811 MS.gene72182:intron 20.0%
! AAACTAATAATATGTTTGGC+TGG - chr4.2:6928224-6928243 MS.gene72182:intron 25.0%
! AACATTTGATCTTCTTCAAA+AGG + chr4.2:6929317-6929336 None:intergenic 25.0%
! AATGGAAGAAATTCAAAAAC+TGG - chr4.2:6927875-6927894 MS.gene72182:CDS 25.0%
! ACATTTGATCTTCTTCAAAA+GGG + chr4.2:6929316-6929335 None:intergenic 25.0%
! ATAATAACATTGCACATTAG+TGG - chr4.2:6929348-6929367 MS.gene72182:intron 25.0%
! ATACTCTTAATCAATGTTCA+CGG + chr4.2:6928554-6928573 None:intergenic 25.0%
! ATGGAAATAGAGATTATAGA+TGG - chr4.2:6929674-6929693 MS.gene72182:intron 25.0%
! GACTTAAATAGATGACATAA+AGG - chr4.2:6928439-6928458 MS.gene72182:intron 25.0%
! GTCAAAATCTTGTATATAGT+TGG - chr4.2:6929750-6929769 MS.gene72182:intron 25.0%
! TAATAACATTGCACATTAGT+GGG - chr4.2:6929349-6929368 MS.gene72182:intron 25.0%
! TACATATCACTTATGTAGAA+TGG - chr4.2:6928501-6928520 MS.gene72182:intron 25.0%
! TGATATGATTGATATAGAGA+AGG - chr4.2:6929954-6929973 MS.gene72182:CDS 25.0%
! TTTCATTTATTGGAAGTTTC+TGG - chr4.2:6928045-6928064 MS.gene72182:CDS 25.0%
!! ATGAAGAAGTTATTTGCTAA+AGG + chr4.2:6928978-6928997 None:intergenic 25.0%
!! GAAGAAGATCAAATGTTTTA+TGG - chr4.2:6929320-6929339 MS.gene72182:intron 25.0%
AAAAACATCACCTATCACTA+AGG + chr4.2:6929717-6929736 None:intergenic 30.0%
AATTCTCATTTCTCACAGAT+TGG - chr4.2:6929239-6929258 MS.gene72182:intron 30.0%
ACCAAAACACCAAATCAATA+TGG + chr4.2:6928765-6928784 None:intergenic 30.0%
ACCAATGAAATTCCACAATT+AGG - chr4.2:6928723-6928742 MS.gene72182:intron 30.0%
AGATTATAGATGGAACTATC+AGG - chr4.2:6929684-6929703 MS.gene72182:intron 30.0%
CATCAAACTCCAAAATATCT+TGG + chr4.2:6928633-6928652 None:intergenic 30.0%
GATTTCATTCTTCTTAGACT+TGG + chr4.2:6930076-6930095 None:intergenic 30.0%
GTTTGCAAATTAAGCATACA+AGG - chr4.2:6928407-6928426 MS.gene72182:intron 30.0%
TAACCTCAAACTTAACAGAT+GGG + chr4.2:6928165-6928184 None:intergenic 30.0%
TCCTAATTGTGGAATTTCAT+TGG + chr4.2:6928727-6928746 None:intergenic 30.0%
TCTATATCAATCATATCACG+AGG + chr4.2:6929952-6929971 None:intergenic 30.0%
TGTTATTGATCTATTGCTTG+AGG - chr4.2:6927979-6927998 MS.gene72182:CDS 30.0%
TTAACCTCAAACTTAACAGA+TGG + chr4.2:6928166-6928185 None:intergenic 30.0%
TTACAAAATCTTGCTCCTTA+AGG - chr4.2:6929482-6929501 MS.gene72182:intron 30.0%
! AGTCATCTTCCAAGATATTT+TGG - chr4.2:6928621-6928640 MS.gene72182:intron 30.0%
! CTATTGCTTGAGGTAATTTT+TGG - chr4.2:6927989-6928008 MS.gene72182:CDS 30.0%
! CTCTTACAAGGTAACTTTTA+CGG - chr4.2:6928917-6928936 MS.gene72182:intron 30.0%
! TAAGGAGCAAGATTTTGTAA+TGG + chr4.2:6929482-6929501 None:intergenic 30.0%
! TCGTCAAAACCATATTGATT+TGG - chr4.2:6928753-6928772 MS.gene72182:intron 30.0%
! TCTTACAAGGTAACTTTTAC+GGG - chr4.2:6928918-6928937 MS.gene72182:intron 30.0%
! TTTTGTCTATAGGCAAAGTA+TGG - chr4.2:6929010-6929029 MS.gene72182:CDS 30.0%
!!! ACCATATTGATTTGGTGTTT+TGG - chr4.2:6928761-6928780 MS.gene72182:CDS 30.0%
!!! CAAGATATTTTGGAGTTTGA+TGG - chr4.2:6928631-6928650 MS.gene72182:intron 30.0%
!!! TAGTTGGCTTTTCAATGAAT+AGG - chr4.2:6929766-6929785 MS.gene72182:intron 30.0%
AACCCAAAAGTAGAATACGA+CGG - chr4.2:6928885-6928904 MS.gene72182:intron 35.0%
AAGCATGCTATGTTGATATG+AGG - chr4.2:6928829-6928848 MS.gene72182:CDS 35.0%
AAGTTCTCAATATGTTAGGG+TGG + chr4.2:6928358-6928377 None:intergenic 35.0%
ACATGATACCGAGACATTAA+GGG - chr4.2:6929619-6929638 MS.gene72182:intron 35.0%
ACATTTCTTACAGCACCTTA+AGG + chr4.2:6929500-6929519 None:intergenic 35.0%
AGCATGCTATGTTGATATGA+GGG - chr4.2:6928830-6928849 MS.gene72182:CDS 35.0%
AGCTTCTTCAACTTATACGT+AGG - chr4.2:6929050-6929069 MS.gene72182:CDS 35.0%
AGGTAGAATGACTTCTGTTA+GGG - chr4.2:6929137-6929156 MS.gene72182:intron 35.0%
AGTTCTCAATATGTTAGGGT+GGG + chr4.2:6928357-6928376 None:intergenic 35.0%
ATCACTTATGTAGAATGGTG+TGG - chr4.2:6928506-6928525 MS.gene72182:intron 35.0%
ATCCACCTTAATGTTTGCTT+TGG + chr4.2:6929893-6929912 None:intergenic 35.0%
ATGCATCTTGTAGATCGTTA+TGG + chr4.2:6928584-6928603 None:intergenic 35.0%
ATTCTTCTTAGACTTGGACT+TGG + chr4.2:6930070-6930089 None:intergenic 35.0%
CAAATTAAGCATACAAGGTC+AGG - chr4.2:6928412-6928431 MS.gene72182:intron 35.0%
CATGCTTCATTTATTTGCTC+TGG + chr4.2:6928816-6928835 None:intergenic 35.0%
CATGTAAACGTCAATGAGAA+TGG + chr4.2:6929604-6929623 None:intergenic 35.0%
GAATAGGAAGTGAATGAACA+AGG + chr4.2:6929542-6929561 None:intergenic 35.0%
GACGAATCAATTCCTAATTG+TGG + chr4.2:6928738-6928757 None:intergenic 35.0%
GAGAAGGATCTTCAAAAGAA+TGG - chr4.2:6929970-6929989 MS.gene72182:CDS 35.0%
GTAAGCTATCTTAAGTAGCA+TGG + chr4.2:6928705-6928724 None:intergenic 35.0%
TAACGATCTACAAGATGCAT+AGG - chr4.2:6928584-6928603 MS.gene72182:intron 35.0%
TACATGATACCGAGACATTA+AGG - chr4.2:6929618-6929637 MS.gene72182:intron 35.0%
TAGCAGAAAACCTAATACTC+CGG - chr4.2:6927829-6927848 MS.gene72182:CDS 35.0%
TCCACCTTAATGTTTGCTTT+GGG + chr4.2:6929892-6929911 None:intergenic 35.0%
! ATAGGCAAAGTATGGTCTTA+AGG - chr4.2:6929018-6929037 MS.gene72182:CDS 35.0%
! CATTTATTGGAAGTTTCTGG+TGG - chr4.2:6928048-6928067 MS.gene72182:CDS 35.0%
! GAGTTTGATGGATGTTATGT+TGG - chr4.2:6928643-6928662 MS.gene72182:intron 35.0%
!! AAGATGTTTTTGAGAGTCTG+AGG - chr4.2:6928859-6928878 MS.gene72182:intron 35.0%
!! TTAAGGTGCTGTAAGAAATG+TGG - chr4.2:6929499-6929518 MS.gene72182:intron 35.0%
!! TTTAGGTTCTGAAAAAGCTC+CGG + chr4.2:6930132-6930151 None:intergenic 35.0%
AAGCCCATCTGTTAAGTTTG+AGG - chr4.2:6928159-6928178 MS.gene72182:intron 40.0%
AAGTGAATGAACAAGGTAGC+AGG + chr4.2:6929535-6929554 None:intergenic 40.0%
AATGAACAAGGTAGCAGGAA+AGG + chr4.2:6929530-6929549 None:intergenic 40.0%
AATTCTCAAGTGACACTGAG+AGG - chr4.2:6927903-6927922 MS.gene72182:CDS 40.0%
ACCCAAAAGTAGAATACGAC+GGG - chr4.2:6928886-6928905 MS.gene72182:intron 40.0%
ACTTATACGTAGGTATCCAG+AGG - chr4.2:6929060-6929079 MS.gene72182:CDS 40.0%
AGGTCATATGCCTTAGTGAT+AGG - chr4.2:6929704-6929723 MS.gene72182:intron 40.0%
AGTTTGGCTAAATCACTGTG+GGG - chr4.2:6928253-6928272 MS.gene72182:intron 40.0%
ATGACCTAAAGCAGCTGTTT+CGG - chr4.2:6929914-6929933 MS.gene72182:CDS 40.0%
CAGGTAGAATGACTTCTGTT+AGG - chr4.2:6929136-6929155 MS.gene72182:intron 40.0%
CCACCTTAATGTTTGCTTTG+GGG + chr4.2:6929891-6929910 None:intergenic 40.0%
CCTTTAGGTCAAAAACAGCA+AGG + chr4.2:6929087-6929106 None:intergenic 40.0%
CTTATACGTAGGTATCCAGA+GGG - chr4.2:6929061-6929080 MS.gene72182:CDS 40.0%
GAAAAAACGTCGTCAGGAAA+TGG + chr4.2:6929184-6929203 None:intergenic 40.0%
GAGTTTGGCTAAATCACTGT+GGG - chr4.2:6928252-6928271 MS.gene72182:intron 40.0%
GTGATATGTAAACGAACCTG+TGG + chr4.2:6928491-6928510 None:intergenic 40.0%
TCACTCTTCCCTTAATGTCT+CGG + chr4.2:6929630-6929649 None:intergenic 40.0%
TGACCCCAAAGCAAACATTA+AGG - chr4.2:6929885-6929904 MS.gene72182:intron 40.0%
TGAGAATGGAAGCAAAGAGA+AGG + chr4.2:6929590-6929609 None:intergenic 40.0%
TGAGTTTGGCTAAATCACTG+TGG - chr4.2:6928251-6928270 MS.gene72182:intron 40.0%
TGCAAGTCTTCCATCACATT+GGG + chr4.2:6929118-6929137 None:intergenic 40.0%
! TTAACAGATGGGCTTCTCAA+AGG + chr4.2:6928154-6928173 None:intergenic 40.0%
!! ATGTTTGCTTTGGGGTCATT+CGG + chr4.2:6929883-6929902 None:intergenic 40.0%
!!! CCTTGCTGTTTTTGACCTAA+AGG - chr4.2:6929084-6929103 MS.gene72182:intron 40.0%
AAAACCTAATACTCCGGTGG+TGG - chr4.2:6927835-6927854 MS.gene72182:CDS 45.0%
AAGATGGGAATTGGTCAGCT+TGG + chr4.2:6930109-6930128 None:intergenic 45.0%
AAGTAGAATACGACGGGCAA+CGG - chr4.2:6928892-6928911 MS.gene72182:intron 45.0%
AATATACGTAAAGCCCCCGT+TGG - chr4.2:6927941-6927960 MS.gene72182:CDS 45.0%
AGGACTGCAGCACAAATTGA+TGG - chr4.2:6930024-6930043 MS.gene72182:CDS 45.0%
CAGAAAACCTAATACTCCGG+TGG - chr4.2:6927832-6927851 MS.gene72182:CDS 45.0%
CATTGGGGTAAGCATCCTTT+AGG + chr4.2:6929102-6929121 None:intergenic 45.0%
CCCCAAAGCAAACATTAAGG+TGG - chr4.2:6929888-6929907 MS.gene72182:intron 45.0%
CTCTGCAAGAGCAACTAGAT+AGG - chr4.2:6927744-6927763 MS.gene72182:CDS 45.0%
CTGAAAAAGCTCCGGAAGAT+GGG + chr4.2:6930124-6930143 None:intergenic 45.0%
CTGCAAGTCTTCCATCACAT+TGG + chr4.2:6929119-6929138 None:intergenic 45.0%
CTTAGACTTGGACTTGGACT+TGG + chr4.2:6930064-6930083 None:intergenic 45.0%
GCAAGTCTTCCATCACATTG+GGG + chr4.2:6929117-6929136 None:intergenic 45.0%
GCTACTGAAAAAACGTCGTC+AGG + chr4.2:6929190-6929209 None:intergenic 45.0%
GCTGCAAATGCATCAGCAAA+TGG - chr4.2:6927857-6927876 MS.gene72182:CDS 45.0%
GCTGTCCAACTTTGATTCAC+AGG - chr4.2:6929843-6929862 MS.gene72182:intron 45.0%
GTCGTCCTGTGAATCAAAGT+TGG + chr4.2:6929851-6929870 None:intergenic 45.0%
TATGTAAACGAACCTGTGGC+AGG + chr4.2:6928487-6928506 None:intergenic 45.0%
TCTCAATATGTTAGGGTGGG+CGG + chr4.2:6928354-6928373 None:intergenic 45.0%
TCTGAAAAAGCTCCGGAAGA+TGG + chr4.2:6930125-6930144 None:intergenic 45.0%
! ATTGGTCAGCTTGGTTCTCT+TGG + chr4.2:6930100-6930119 None:intergenic 45.0%
! CAATGTGATGGAAGACTTGC+AGG - chr4.2:6929117-6929136 MS.gene72182:intron 45.0%
! TACTCCGAAACAGCTGCTTT+AGG + chr4.2:6929921-6929940 None:intergenic 45.0%
! TGCCCGTCGTATTCTACTTT+TGG + chr4.2:6928891-6928910 None:intergenic 45.0%
! TTTTCCCTCGTCTCATGCAT+CGG + chr4.2:6928090-6928109 None:intergenic 45.0%
!! ACGGTTTTAATGCCAAGGGT+TGG + chr4.2:6929403-6929422 None:intergenic 45.0%
!! CGGTTTTAATGCCAAGGGTT+GGG + chr4.2:6929402-6929421 None:intergenic 45.0%
!! GCCCGTCGTATTCTACTTTT+GGG + chr4.2:6928890-6928909 None:intergenic 45.0%
!! GGCAACGGTTTTAATGCCAA+GGG + chr4.2:6929407-6929426 None:intergenic 45.0%
!! TGGCAACGGTTTTAATGCCA+AGG + chr4.2:6929408-6929427 None:intergenic 45.0%
!! TGTAGGCTTTGAAAGCTGCA+GGG - chr4.2:6929809-6929828 MS.gene72182:intron 45.0%
!! TGTTTGCTTTGGGGTCATTC+GGG + chr4.2:6929882-6929901 None:intergenic 45.0%
!! TTGTAGGCTTTGAAAGCTGC+AGG - chr4.2:6929808-6929827 MS.gene72182:intron 45.0%
!!! CGACGTTTTTTCAGTAGCAG+TGG - chr4.2:6929192-6929211 MS.gene72182:intron 45.0%
!!! TTCCACTTTTTCCCAACCCT+TGG - chr4.2:6929388-6929407 MS.gene72182:intron 45.0%
AAACGAACCTGTGGCAGGAT+TGG + chr4.2:6928482-6928501 None:intergenic 50.0%
AACGAACCTGTGGCAGGATT+GGG + chr4.2:6928481-6928500 None:intergenic 50.0%
AAGCTCCGGAAGATGGGAAT+TGG + chr4.2:6930118-6930137 None:intergenic 50.0%
ACGCTTCATCTGAGCACCAA+CGG + chr4.2:6927960-6927979 None:intergenic 50.0%
AGCATTGCGTCGAGCAAAGT+TGG - chr4.2:6927806-6927825 MS.gene72182:CDS 50.0%
AGTCCTCCTCTTTGCAGTGT+TGG + chr4.2:6930010-6930029 None:intergenic 50.0%
CTACCAACACTGCAAAGAGG+AGG - chr4.2:6930004-6930023 MS.gene72182:CDS 50.0%
CTGCTACCAACACTGCAAAG+AGG - chr4.2:6930001-6930020 MS.gene72182:CDS 50.0%
CTTGGACTTGGACTTGGACT+TGG + chr4.2:6930058-6930077 None:intergenic 50.0%
GATTCCGATGCATGAGACGA+GGG - chr4.2:6928083-6928102 MS.gene72182:intron 50.0%
GCTGACCAATTCCCATCTTC+CGG - chr4.2:6930110-6930129 MS.gene72182:CDS 50.0%
GGATGCTTACCCCAATGTGA+TGG - chr4.2:6929105-6929124 MS.gene72182:intron 50.0%
GTTTGGCTGGACACTGAGTT+TGG - chr4.2:6928237-6928256 MS.gene72182:intron 50.0%
TCAAAAACAGCAAGGCCCTC+TGG + chr4.2:6929079-6929098 None:intergenic 50.0%
TCAAATCCCAATCCTGCCAC+AGG - chr4.2:6928472-6928491 MS.gene72182:intron 50.0%
TGAAAGCTGCAGGGCAGATT+TGG - chr4.2:6929818-6929837 MS.gene72182:intron 50.0%
TGATTCCGATGCATGAGACG+AGG - chr4.2:6928082-6928101 MS.gene72182:intron 50.0%
TGCCAAGGGTTGGGAAAAAG+TGG + chr4.2:6929393-6929412 None:intergenic 50.0%
!! CTCTTTGCAGTGTTGGTAGC+AGG + chr4.2:6930003-6930022 None:intergenic 50.0%
CGCTTCATCTGAGCACCAAC+GGG + chr4.2:6927959-6927978 None:intergenic 55.0%
CGGGCAACGGTTCTCTTACA+AGG - chr4.2:6928905-6928924 MS.gene72182:intron 55.0%
CTTCATCTGAGCACCAACGG+GGG + chr4.2:6927957-6927976 None:intergenic 55.0%
GATGCATTTGCAGCCACCAC+CGG + chr4.2:6927851-6927870 None:intergenic 55.0%
GCTTCATCTGAGCACCAACG+GGG + chr4.2:6927958-6927977 None:intergenic 55.0%
! GCAGCCACCACCGGAGTATT+AGG + chr4.2:6927842-6927861 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 6927740 6930155 6927740 ID=MS.gene72182
chr4.2 mRNA 6927740 6930155 6927740 ID=MS.gene72182.t1;Parent=MS.gene72182
chr4.2 exon 6929895 6930155 6929895 ID=MS.gene72182.t1.exon1;Parent=MS.gene72182.t1
chr4.2 CDS 6929895 6930155 6929895 ID=cds.MS.gene72182.t1;Parent=MS.gene72182.t1
chr4.2 exon 6928969 6929100 6928969 ID=MS.gene72182.t1.exon2;Parent=MS.gene72182.t1
chr4.2 CDS 6928969 6929100 6928969 ID=cds.MS.gene72182.t1;Parent=MS.gene72182.t1
chr4.2 exon 6928757 6928873 6928757 ID=MS.gene72182.t1.exon3;Parent=MS.gene72182.t1
chr4.2 CDS 6928757 6928873 6928757 ID=cds.MS.gene72182.t1;Parent=MS.gene72182.t1
chr4.2 exon 6927740 6928081 6927740 ID=MS.gene72182.t1.exon4;Parent=MS.gene72182.t1
chr4.2 CDS 6927740 6928081 6927740 ID=cds.MS.gene72182.t1;Parent=MS.gene72182.t1
Gene Sequence

>MS.gene72182

ATGGCTCTGCAAGAGCAACTAGATAGGTTCAAGAAACAACAAGAGAAGTGTCAATCGACACTCTCGAGCATTGCGTCGAGCAAAGTTGGTAGCAGAAAACCTAATACTCCGGTGGTGGCTGCAAATGCATCAGCAAATGGAAGAAATTCAAAAACTGGTGTTAAATTCTCAAGTGACACTGAGAGGCTTCAGCAGATTAATAATATACGTAAAGCCCCCGTTGGTGCTCAGATGAAGCGTGTTATTGATCTATTGCTTGAGGTAATTTTTGGTTTTCTCATTCAATTCAATTTTGTTGTTTGTTTTTTCATTTATTGGAAGTTTCTGGTGGTGTTATTGCAGTGATTCCGATGCATGAGACGAGGGAAAAAAAATGAATTTTATTAGAATATATACACTCATTTTACATATCCTTTGAGAAGCCCATCTGTTAAGTTTGAGGTTAATTGAGACACTATAGCTCAAAGTCACCAATTGCAAATTGAAACTAATAATATGTTTGGCTGGACACTGAGTTTGGCTAAATCACTGTGGGGCTGTGATTTTGTTGAAGCTTCAACGTGTAGCTTTTGCTGAAATCGCTGTTACGCACTGTGATTTCGTCAAACTCACCGCCCACCCTAACATATTGAGAACTTAATTTAAGAAGCAAAAACGTTTTTTTATTGTTTGCAAATTAAGCATACAAGGTCAGGTTATGACTTAAATAGATGACATAAAGGGTCAATTTGTTCAAATCCCAATCCTGCCACAGGTTCGTTTACATATCACTTATGTAGAATGGTGTGGTACGTAGTTTGAAAATTGTATTCCGTGAACATTGATTAAGAGTATTTTTTTTCCATAACGATCTACAAGATGCATAGGTATGCTTTACTTGCAGTCATCTTCCAAGATATTTTGGAGTTTGATGGATGTTATGTTGGTTCTCTTAAACTGAGTTGAATTCTTATTTTGAAGAGCCATGCTACTTAAGATAGCTTACCAATGAAATTCCACAATTAGGAATTGATTCGTCAAAACCATATTGATTTGGTGTTTTGGTTTGCTGATAGACACGACAAGCTTTTACACCAGAGCAAATAAATGAAGCATGCTATGTTGATATGAGGGCTAACAAAGATGTTTTTGAGAGTCTGAGGAAAAACCCAAAAGTAGAATACGACGGGCAACGGTTCTCTTACAAGGTAACTTTTACGGGTTCATATATCTTAATAGTTTTATATTTAAATACTCCTTTAGCAAATAACTTCTTCATCTGTTATTTGTTTTTTGTCTATAGGCAAAGTATGGTCTTAAGGAGAAAAGCGAGCTTCTTCAACTTATACGTAGGTATCCAGAGGGCCTTGCTGTTTTTGACCTAAAGGATGCTTACCCCAATGTGATGGAAGACTTGCAGGTAGAATGACTTCTGTTAGGGCTGTTTTCTTTTCTTTGAAGACCATTTCCTGACGACGTTTTTTCAGTAGCAGTGGCTGTTTTGTTCATAGATATATGTTAATTCTCATTTCTCACAGATTGGACTTTTGTCTAGATAATGTGTTTTGTAAGAAATATTAACTGAAACACTATACCCTTTTGAAGAAGATCAAATGTTTTATGGGTAATATAATAACATTGCACATTAGTGGGTTTTATAAAATCGTTTTTCCACTTTTTCCCAACCCTTGGCATTAAAACCGTTGCCATTATTATAAATTAAGGTTAATACTGAGGAAATTGAAACATCTTATAATTAGCCATTACAAAATCTTGCTCCTTAAGGTGCTGTAAGAAATGTGGTTCAACCTTTCCTGCTACCTTGTTCATTCACTTCCTATTCATTTGTTTTGTTTATTTTATAAAGACCTTCTCTTTGCTTCCATTCTCATTGACGTTTACATGATACCGAGACATTAAGGGAAGAGTGATTTTTTTAATGTTTGTCTTGAAATATGGAAATAGAGATTATAGATGGAACTATCAGGTCATATGCCTTAGTGATAGGTGATGTTTTTGTAGAAGTGAAGAGTCAAAATCTTGTATATAGTTGGCTTTTCAATGAATAGGATCTCATAATGATATCTTTTTGTAGGCTTTGAAAGCTGCAGGGCAGATTTGGCTGCTGTCCAACTTTGATTCACAGGACGACATTGCATACCCGAATGACCCCAAAGCAAACATTAAGGTGGATGATGACCTAAAGCAGCTGTTTCGGAGTATTGAGTTACCTCGTGATATGATTGATATAGAGAAGGATCTTCAAAAGAATGGAATGAAGCCTGCTACCAACACTGCAAAGAGGAGGACTGCAGCACAAATTGATGGCGTTTCTTCCAAGTCCAAGTCCAAGTCCAAGTCTAAGAAGAATGAAATCACCAAGAGAACCAAGCTGACCAATTCCCATCTTCCGGAGCTTTTTCAGAACCTAAAGTAA
Protein sequence

>MS.gene72182.t1

MALQEQLDRFKKQQEKCQSTLSSIASSKVGSRKPNTPVVAANASANGRNSKTGVKFSSDTERLQQINNIRKAPVGAQMKRVIDLLLETRQAFTPEQINEACYVDMRANKDVFESLRKNPKVEYDGQRFSYKAKYGLKEKSELLQLIRRYPEGLAVFDLKDAYPNVMEDLQALKAAGQIWLLSNFDSQDDIAYPNDPKANIKVDDDLKQLFRSIELPRDMIDIEKDLQKNGMKPATNTAKRRTAAQIDGVSSKSKSKSKSKKNEITKRTKLTNSHLPELFQNLK