Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72658.t1 | XP_003612368.2 | 96 | 1232 | 48 | 1 | 1 | 1231 | 1 | 1232 | 0.00E+00 | 2354.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72658.t1 | F4JN26 | 37.0 | 1295 | 612 | 27 | 1 | 1231 | 1 | 1155 | 2.1e-206 | 721.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72658.t1 | G7K187 | 96.0 | 1232 | 48 | 1 | 1 | 1231 | 1 | 1232 | 0.0e+00 | 2354.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052190 | MS.gene72658 | 0.828177 | 1.04E-54 | -1.69E-46 |
| MS.gene053166 | MS.gene72658 | 0.812997 | 3.20E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene72658 | 0.803813 | 2.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72658 | MS.gene96593 | PPI |
| MS.gene009300 | MS.gene72658 | PPI |
| MS.gene72658 | MS.gene058795 | PPI |
| MS.gene72658 | MS.gene52850 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72658.t1 | MTR_5g024250 | 95.779 | 1232 | 51 | 1 | 1 | 1231 | 1 | 1232 | 0.0 | 2444 |
| MS.gene72658.t1 | MTR_1g088720 | 24.798 | 371 | 244 | 9 | 4 | 367 | 8 | 350 | 1.83e-24 | 111 |
| MS.gene72658.t1 | MTR_3g005810 | 31.618 | 272 | 166 | 8 | 4 | 264 | 7 | 269 | 9.56e-22 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72658.t1 | AT4G35520 | 50.820 | 427 | 198 | 3 | 1 | 418 | 1 | 424 | 1.12e-130 | 431 |
| MS.gene72658.t1 | AT4G35520 | 54.545 | 418 | 168 | 10 | 820 | 1231 | 779 | 1180 | 3.57e-128 | 424 |
| MS.gene72658.t1 | AT4G35520 | 54.286 | 420 | 170 | 10 | 818 | 1231 | 528 | 931 | 8.22e-130 | 422 |
| MS.gene72658.t1 | AT4G35520 | 36.686 | 169 | 97 | 2 | 259 | 418 | 8 | 175 | 4.28e-25 | 113 |
| MS.gene72658.t1 | AT4G35520 | 57.714 | 350 | 146 | 1 | 1 | 350 | 1 | 348 | 6.28e-129 | 425 |
| MS.gene72658.t1 | AT4G35520 | 54.545 | 418 | 168 | 10 | 820 | 1231 | 752 | 1153 | 2.17e-128 | 424 |
| MS.gene72658.t1 | AT4G35520 | 54.545 | 418 | 168 | 10 | 820 | 1231 | 742 | 1143 | 1.91e-128 | 424 |
| MS.gene72658.t1 | AT4G35520 | 45.199 | 427 | 185 | 3 | 1 | 418 | 1 | 387 | 5.69e-106 | 362 |
| MS.gene72658.t1 | AT4G35520 | 54.545 | 418 | 168 | 10 | 820 | 1231 | 754 | 1155 | 2.24e-128 | 424 |
| MS.gene72658.t1 | AT4G35520 | 48.276 | 377 | 156 | 1 | 1 | 377 | 1 | 338 | 1.88e-104 | 357 |
| MS.gene72658.t1 | AT4G35520 | 52.535 | 434 | 170 | 11 | 818 | 1231 | 540 | 957 | 5.32e-126 | 412 |
| MS.gene72658.t1 | AT4G35520 | 42.857 | 119 | 68 | 0 | 259 | 377 | 8 | 126 | 8.79e-24 | 109 |
| MS.gene72658.t1 | AT4G35520 | 52.778 | 432 | 168 | 11 | 820 | 1231 | 742 | 1157 | 1.19e-124 | 414 |
| MS.gene72658.t1 | AT4G35520 | 45.199 | 427 | 185 | 3 | 1 | 418 | 1 | 387 | 8.83e-106 | 361 |
| MS.gene72658.t1 | AT4G35520 | 45.199 | 427 | 185 | 3 | 1 | 418 | 1 | 387 | 4.07e-106 | 360 |
| MS.gene72658.t1 | AT4G35520 | 50.000 | 346 | 156 | 9 | 820 | 1159 | 742 | 1076 | 1.16e-87 | 308 |
| MS.gene72658.t1 | AT4G09140 | 32.857 | 280 | 152 | 10 | 4 | 264 | 29 | 291 | 3.61e-23 | 107 |
| MS.gene72658.t1 | AT4G02460 | 26.485 | 404 | 231 | 14 | 4 | 386 | 17 | 375 | 4.69e-23 | 106 |
| MS.gene72658.t1 | AT4G02460 | 26.485 | 404 | 231 | 14 | 4 | 386 | 17 | 375 | 5.84e-23 | 106 |
Find 254 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCATATATCTTGAATTTA+AGG | 0.086807 | 5.4:-15693759 | MS.gene72658:CDS |
| TACATCGAAATTTCTTAATT+TGG | 0.131266 | 5.4:-15695674 | MS.gene72658:CDS |
| ACAACTTTGAAGGAGATATT+TGG | 0.162274 | 5.4:+15694430 | None:intergenic |
| TGACTATCTTTGCAGGAATT+TGG | 0.205955 | 5.4:-15687432 | MS.gene72658:intron |
| ATTGATTGAAGTACCTTATT+GGG | 0.214424 | 5.4:+15694487 | None:intergenic |
| GATTGATTGAAGTACCTTAT+TGG | 0.215758 | 5.4:+15694486 | None:intergenic |
| GTGCAAGATTCCGGAGATTA+TGG | 0.216270 | 5.4:-15689039 | MS.gene72658:CDS |
| CGGTTATTCTGGCAATTTAC+TGG | 0.226743 | 5.4:-15691105 | MS.gene72658:CDS |
| CACTTGTAACCACTGGCTTT+GGG | 0.232639 | 5.4:-15694277 | MS.gene72658:CDS |
| TTGAATATTTAGGTATTATC+TGG | 0.241070 | 5.4:-15687946 | MS.gene72658:intron |
| GACCCTGCTTCCAAACTTCT+AGG | 0.247169 | 5.4:-15689871 | MS.gene72658:intron |
| ATCAACTACTTCATAGTTAC+AGG | 0.248733 | 5.4:-15687773 | MS.gene72658:CDS |
| AACTCCAAACCGATAGCTTT+TGG | 0.249668 | 5.4:-15690731 | MS.gene72658:CDS |
| TCACTTGTAACCACTGGCTT+TGG | 0.249770 | 5.4:-15694278 | MS.gene72658:CDS |
| TCTGGATTCTTGCATCTTGC+TGG | 0.260504 | 5.4:-15688803 | MS.gene72658:CDS |
| CTTCGTCAGACCGGTTATTC+TGG | 0.261638 | 5.4:-15691116 | MS.gene72658:CDS |
| TCTTAAGGTGCGATTATGTT+TGG | 0.264068 | 5.4:-15686860 | MS.gene72658:intron |
| CATTTCTTACTGATAGTATC+AGG | 0.266001 | 5.4:+15688767 | None:intergenic |
| TTCAACAAAATGATCCATTC+AGG | 0.266010 | 5.4:-15689499 | MS.gene72658:CDS |
| AATATACTTGACATCTCTTC+TGG | 0.266206 | 5.4:-15688821 | MS.gene72658:CDS |
| CTTAAGGTGCGATTATGTTT+GGG | 0.269942 | 5.4:-15686859 | MS.gene72658:intron |
| CCTCAAAGAGAAACAAAATA+AGG | 0.270902 | 5.4:-15691147 | MS.gene72658:CDS |
| GTTTCAGTGTTTGTTGATAT+TGG | 0.273741 | 5.4:-15696787 | MS.gene72658:CDS |
| CATTTCTGAAGTCTCTTTGT+TGG | 0.291486 | 5.4:-15695596 | MS.gene72658:CDS |
| AACGATATCATAAAGCTTAC+TGG | 0.297572 | 5.4:-15694215 | MS.gene72658:CDS |
| CATAGCGGTGGACATGCTTT+TGG | 0.298068 | 5.4:+15691070 | None:intergenic |
| AAAAGGTCATCAAACAGTTC+TGG | 0.303292 | 5.4:-15691710 | MS.gene72658:CDS |
| GAAACGGAGTTCAGAGGTTC+TGG | 0.308935 | 5.4:-15689576 | MS.gene72658:CDS |
| AACAGACCCAACATTATAAA+AGG | 0.310471 | 5.4:-15690051 | MS.gene72658:CDS |
| TCCAATAGATCTTCATTGTT+GGG | 0.311658 | 5.4:+15689713 | None:intergenic |
| CTAAACAATCCTTACGATCT+TGG | 0.315143 | 5.4:+15691276 | None:intergenic |
| CAAGCAAAGTCTTCTCGTTC+AGG | 0.317110 | 5.4:+15690769 | None:intergenic |
| CCCCAACAATGAAGATCTAT+TGG | 0.321390 | 5.4:-15689714 | MS.gene72658:CDS |
| TTCTAACACACTCAGGTAAT+AGG | 0.325451 | 5.4:+15690079 | None:intergenic |
| CTCCAACAAAGCCACTTATA+TGG | 0.326027 | 5.4:-15691569 | MS.gene72658:CDS |
| ATGATACACATTTCACAAAA+AGG | 0.328678 | 5.4:-15690131 | MS.gene72658:CDS |
| ACCAATGATATTAATGATCT+TGG | 0.347298 | 5.4:-15690438 | MS.gene72658:CDS |
| CACTGTTCCTTTAGTCAACT+TGG | 0.348856 | 5.4:-15686667 | MS.gene72658:CDS |
| TTGTTTCAGAATTGATATCT+AGG | 0.349287 | 5.4:-15689065 | MS.gene72658:intron |
| CAACTTTGAAGGAGATATTT+GGG | 0.351489 | 5.4:+15694431 | None:intergenic |
| TTCAATATCAACAACTTTGA+AGG | 0.351846 | 5.4:+15694420 | None:intergenic |
| CATGCAGATATATCAAAGTT+TGG | 0.352223 | 5.4:-15691302 | MS.gene72658:intron |
| TCCAAGATCATTAATATCAT+TGG | 0.357505 | 5.4:+15690437 | None:intergenic |
| GCATACATCCCTCTGTTTCC+AGG | 0.358909 | 5.4:-15686789 | MS.gene72658:intron |
| CCGGAGATTATGGGACTAAA+TGG | 0.359448 | 5.4:-15689029 | MS.gene72658:CDS |
| CAACATTATAAAAGGCGAAT+TGG | 0.362809 | 5.4:-15690043 | MS.gene72658:CDS |
| GAAATAATGACAAGAACTTA+TGG | 0.365886 | 5.4:-15695574 | MS.gene72658:CDS |
| CTCCTTCTACTTCTATGAAA+TGG | 0.368433 | 5.4:+15689955 | None:intergenic |
| GCTTTAGCTCTTCAACTAGA+AGG | 0.372548 | 5.4:+15686810 | None:intergenic |
| TGGATTTGTTTCCTCCGAAC+TGG | 0.374132 | 5.4:+15694643 | None:intergenic |
| AAAATTCTGAATCCTTTAGA+AGG | 0.374608 | 5.4:-15687707 | MS.gene72658:intron |
| TTTACCTGCTTTGGCTGAAA+TGG | 0.374834 | 5.4:+15691513 | None:intergenic |
| CAACAAAGAGACTTCAGAAA+TGG | 0.375063 | 5.4:+15695597 | None:intergenic |
| ATTGAGCTACGAACAGATTC+CGG | 0.378058 | 5.4:+15696985 | None:intergenic |
| AACCTATCTAGATGCTGAAC+AGG | 0.381349 | 5.4:-15687908 | MS.gene72658:CDS |
| TCTAGGGAAGTGCAAGATTC+CGG | 0.388056 | 5.4:-15689048 | MS.gene72658:CDS |
| AATACTTTCCTATATCCATT+TGG | 0.389721 | 5.4:+15695547 | None:intergenic |
| CAGTAAATTGCCAGAATAAC+CGG | 0.392348 | 5.4:+15691106 | None:intergenic |
| AACCATATAAGTGGCTTTGT+TGG | 0.392990 | 5.4:+15691567 | None:intergenic |
| CAGGGAACAGATTAAAGATT+GGG | 0.394408 | 5.4:-15687754 | MS.gene72658:CDS |
| CAGGTCCCTTGCAAACGAAC+TGG | 0.395404 | 5.4:+15693887 | None:intergenic |
| AACCCTTGCTGTTATTGATC+AGG | 0.398168 | 5.4:-15688681 | MS.gene72658:intron |
| AATATTATCATAGAAACTTT+CGG | 0.404601 | 5.4:+15690965 | None:intergenic |
| ATCGAAATTTCTTAATTTGG+CGG | 0.404648 | 5.4:-15695671 | MS.gene72658:CDS |
| TACTCGAGATGGGCTGGAAT+TGG | 0.405515 | 5.4:-15696464 | MS.gene72658:CDS |
| ATTGTCTCCCTCTAGATTCA+TGG | 0.408060 | 5.4:-15690575 | MS.gene72658:CDS |
| CTGAATTGGAAGCCCCAATA+AGG | 0.417478 | 5.4:-15694500 | MS.gene72658:intron |
| AGAAAGGAAGTAGGCACAAC+AGG | 0.420276 | 5.4:-15695394 | MS.gene72658:intron |
| AACCACTGGCTTTGGGGTGG+AGG | 0.425969 | 5.4:-15694270 | MS.gene72658:CDS |
| TACTCCAAAAGCTATCGGTT+TGG | 0.428894 | 5.4:+15690727 | None:intergenic |
| TAAAGAAATATTCAACAGAT+TGG | 0.428963 | 5.4:-15690172 | MS.gene72658:CDS |
| ATGACAATCATCAGTGCTAC+AGG | 0.429264 | 5.4:-15691217 | MS.gene72658:CDS |
| AGGGTTCTGCCAGCCATGAT+TGG | 0.431893 | 5.4:+15688698 | None:intergenic |
| CATGTCAAGGAATGCTAACC+AGG | 0.437046 | 5.4:-15690253 | MS.gene72658:CDS |
| CTTAAATTCAAGATATATGC+AGG | 0.440812 | 5.4:+15693760 | None:intergenic |
| AAGCTTACTGGATACATATC+AGG | 0.441992 | 5.4:-15694203 | MS.gene72658:CDS |
| TTCCAATAGATCTTCATTGT+TGG | 0.442789 | 5.4:+15689712 | None:intergenic |
| TTCATTTAGCTTGTCTGAAG+TGG | 0.442895 | 5.4:+15691241 | None:intergenic |
| ATGGACGGCCAAATGGATAT+AGG | 0.443464 | 5.4:-15695555 | MS.gene72658:CDS |
| TTTAAATCAGCCTCCCGAAA+TGG | 0.444086 | 5.4:-15689923 | MS.gene72658:CDS |
| ATGCAGGTACTGCCAGAGAT+TGG | 0.445165 | 5.4:-15687798 | MS.gene72658:intron |
| CTCCATTTCATAGAAGTAGA+AGG | 0.445805 | 5.4:-15689957 | MS.gene72658:CDS |
| TTCATTGTGCAGTGTGCTCA+TGG | 0.448403 | 5.4:-15686699 | MS.gene72658:intron |
| CACTCAGGTAATAGGCAGTC+TGG | 0.454013 | 5.4:+15690087 | None:intergenic |
| TCATAGAAACTTTCGGCAGC+AGG | 0.454172 | 5.4:+15690972 | None:intergenic |
| AAGAGAAAAGCAACCTGGAT+TGG | 0.454580 | 5.4:+15694623 | None:intergenic |
| GTAGAGCAACTCTTTAGAAA+AGG | 0.454936 | 5.4:+15690807 | None:intergenic |
| GGTTATGAGTGCCATGGGTT+CGG | 0.455266 | 5.4:-15690864 | MS.gene72658:CDS |
| TGGTATTCAGCAACACAAAC+AGG | 0.455374 | 5.4:-15690555 | MS.gene72658:CDS |
| TGCAAGATTCCGGAGATTAT+GGG | 0.455509 | 5.4:-15689038 | MS.gene72658:CDS |
| TGACTTAAAAGCTTATGAAC+AGG | 0.455665 | 5.4:+15693868 | None:intergenic |
| GGCGACGATAAGACCTTTAC+CGG | 0.456254 | 5.4:-15697004 | MS.gene72658:CDS |
| GCAACACAAACAGGTAACTT+TGG | 0.459066 | 5.4:-15690546 | MS.gene72658:CDS |
| AAAGGTCATCAAACAGTTCT+GGG | 0.460494 | 5.4:-15691709 | MS.gene72658:CDS |
| TATGAATCACCTTCATATCC+AGG | 0.462141 | 5.4:+15694168 | None:intergenic |
| AATGACGCTGCTGAGATTGA+AGG | 0.462435 | 5.4:-15689539 | MS.gene72658:CDS |
| TATTCAACAGATTGGTTGAC+TGG | 0.463602 | 5.4:-15690164 | MS.gene72658:CDS |
| ATCCACCTGATGAAGAACTT+TGG | 0.466886 | 5.4:+15688733 | None:intergenic |
| CTCAAAGAGAAACAAAATAA+GGG | 0.467320 | 5.4:-15691146 | MS.gene72658:CDS |
| TGGATATAGGAAAGTATTGA+AGG | 0.472768 | 5.4:-15695542 | MS.gene72658:intron |
| CTACGAACAGATTCCGGTAA+AGG | 0.473028 | 5.4:+15696991 | None:intergenic |
| AGAACTTATGGACGGCCAAA+TGG | 0.473558 | 5.4:-15695562 | MS.gene72658:CDS |
| ATCTTCTTGAACCATATAAG+TGG | 0.476038 | 5.4:+15691558 | None:intergenic |
| ACAGGGAACAGATTAAAGAT+TGG | 0.476385 | 5.4:-15687755 | MS.gene72658:CDS |
| TGGAATTACTCGAGATGGGC+TGG | 0.478152 | 5.4:-15696470 | MS.gene72658:CDS |
| TTGTTGGGGCTAAGAGCGTC+AGG | 0.478488 | 5.4:+15689728 | None:intergenic |
| TCAAAGTTGTTGATATTGAA+AGG | 0.484148 | 5.4:-15694417 | MS.gene72658:intron |
| CATAATCAAATAGCCAAGCT+TGG | 0.487009 | 5.4:-15686636 | MS.gene72658:CDS |
| AAATGTGTATCATGGAAATC+AGG | 0.487329 | 5.4:+15690141 | None:intergenic |
| CTTTAGCTCTTCAACTAGAA+GGG | 0.488271 | 5.4:+15686811 | None:intergenic |
| AATACTAAACTTCATTGAAA+AGG | 0.489534 | 5.4:-15691727 | MS.gene72658:CDS |
| TTGCTTACTCCAAAAGCTAT+CGG | 0.489893 | 5.4:+15690722 | None:intergenic |
| GGATGCCAAAGTTCTTCATC+AGG | 0.491605 | 5.4:-15688738 | MS.gene72658:CDS |
| CATATAAGTGGCTTTGTTGG+AGG | 0.492033 | 5.4:+15691570 | None:intergenic |
| GATAACAATGATTTATTGCA+AGG | 0.492434 | 5.4:-15691020 | MS.gene72658:CDS |
| CTTGTTCATCAGTCCAAGCT+TGG | 0.495904 | 5.4:+15686623 | None:intergenic |
| ACCTGCTTTGGCTGAAATGG+TGG | 0.497376 | 5.4:+15691516 | None:intergenic |
| GAATTGGTGGGAGAAAGATA+TGG | 0.497867 | 5.4:-15696448 | MS.gene72658:intron |
| TCGAGATGGGCTGGAATTGG+TGG | 0.499643 | 5.4:-15696461 | MS.gene72658:CDS |
| CCACTGTCACTTGTAACCAC+TGG | 0.501918 | 5.4:-15694284 | MS.gene72658:CDS |
| AGGGTCGGATGCTAGACCGC+TGG | 0.502251 | 5.4:+15689888 | None:intergenic |
| ATCATAGAACTAATGTGAAA+CGG | 0.504755 | 5.4:-15689592 | MS.gene72658:intron |
| AACTTGAGGTGCATGGATCC+TGG | 0.505219 | 5.4:+15690235 | None:intergenic |
| ATCGGTTATCAAACTCTGAA+TGG | 0.506997 | 5.4:-15690515 | MS.gene72658:CDS |
| GTAACTTTGGTTCTGATGAT+CGG | 0.510715 | 5.4:-15690533 | MS.gene72658:CDS |
| CTCCTGTTCAGCATCTAGAT+AGG | 0.511106 | 5.4:+15687906 | None:intergenic |
| GGAGGTGGAATTACTCGAGA+TGG | 0.511633 | 5.4:-15696475 | MS.gene72658:CDS |
| AACAGATTAAAGATTGGGGC+TGG | 0.512814 | 5.4:-15687749 | MS.gene72658:CDS |
| CCAATAGATCTTCATTGTTG+GGG | 0.514398 | 5.4:+15689714 | None:intergenic |
| TTGAAGGTTATGAGTGCCAT+GGG | 0.514841 | 5.4:-15690869 | MS.gene72658:CDS |
| CAACTCCCAGTTCGTTTGCA+AGG | 0.515346 | 5.4:-15693893 | MS.gene72658:CDS |
| CTGTGAGATATACCTTCTAA+AGG | 0.516124 | 5.4:+15687695 | None:intergenic |
| GTTTGGAAGCCAAGATCGTA+AGG | 0.516892 | 5.4:-15691285 | MS.gene72658:CDS |
| TACTTAAAAGTAGTTGATGA+TGG | 0.517169 | 5.4:-15696757 | MS.gene72658:intron |
| GGGTGCAAGTGTATATATCT+TGG | 0.517916 | 5.4:-15695430 | MS.gene72658:CDS |
| GCTCAAAACATATAAAGAGC+GGG | 0.517968 | 5.4:+15693732 | None:intergenic |
| TTGCAATACCCTGGATATGA+AGG | 0.518092 | 5.4:-15694177 | MS.gene72658:intron |
| AAACCTAGAAGTTTGGAAGC+AGG | 0.519365 | 5.4:+15689868 | None:intergenic |
| TGCAACTTCTACACAGGGAC+GGG | 0.520833 | 5.4:+15690656 | None:intergenic |
| GGTTATGATGTAGAGATTGG+TGG | 0.521108 | 5.4:-15690843 | MS.gene72658:CDS |
| ACATTGAACTCACCCCTGAA+TGG | 0.521481 | 5.4:+15689485 | None:intergenic |
| TATTCTACAACCAACCAGTT+CGG | 0.522442 | 5.4:-15694657 | MS.gene72658:CDS |
| ACTGGAAGACTTGCGTCAAA+AGG | 0.523475 | 5.4:-15688029 | MS.gene72658:intron |
| AACTCCCAGTTCGTTTGCAA+GGG | 0.524126 | 5.4:-15693892 | MS.gene72658:CDS |
| AGTAGTTGATAACCAATCTC+TGG | 0.524732 | 5.4:+15687786 | None:intergenic |
| TTCGGTTATGATGTAGAGAT+TGG | 0.525279 | 5.4:-15690846 | MS.gene72658:CDS |
| TGTAACCACTGGCTTTGGGG+TGG | 0.527919 | 5.4:-15694273 | MS.gene72658:CDS |
| GAAGAACTTCTCGCATAAGA+AGG | 0.530402 | 5.4:+15690942 | None:intergenic |
| TGGCATTGATGATGATAGAA+AGG | 0.533656 | 5.4:-15695410 | MS.gene72658:CDS |
| CCTCGATGCTCGCGCTACAA+AGG | 0.534912 | 5.4:-15696902 | MS.gene72658:intron |
| GGGTCGGATGCTAGACCGCT+GGG | 0.537874 | 5.4:+15689889 | None:intergenic |
| TCCACTATTTGCAAAACTTG+AGG | 0.543285 | 5.4:+15690221 | None:intergenic |
| AAATTAAATGAAGAAGATGC+TGG | 0.543458 | 5.4:-15690909 | MS.gene72658:CDS |
| ATGGATACAGAGATACTGTA+AGG | 0.543591 | 5.4:+15694035 | None:intergenic |
| GCCACCATTTCAGCCAAAGC+AGG | 0.544008 | 5.4:-15691517 | MS.gene72658:intron |
| TCAACAAAATGATCCATTCA+GGG | 0.544139 | 5.4:-15689498 | MS.gene72658:CDS |
| TGTTTCAGAATTGATATCTA+GGG | 0.546621 | 5.4:-15689064 | MS.gene72658:intron |
| CCATTTAGTCCCATAATCTC+CGG | 0.546747 | 5.4:+15689029 | None:intergenic |
| TTTGCAAAACTTGAGGTGCA+TGG | 0.547477 | 5.4:+15690228 | None:intergenic |
| ACTTGTAACCACTGGCTTTG+GGG | 0.548380 | 5.4:-15694276 | MS.gene72658:CDS |
| AAATTGACACCTAAAATACA+TGG | 0.549038 | 5.4:+15687262 | None:intergenic |
| TCTAGATGCTGAACAGGAGC+TGG | 0.549469 | 5.4:-15687902 | MS.gene72658:intron |
| AAATTCATTCCAATCATGGC+TGG | 0.550857 | 5.4:-15688707 | MS.gene72658:CDS |
| ATCATCAACAATGCCACCCT+CGG | 0.553332 | 5.4:-15687088 | MS.gene72658:CDS |
| GTGATCTGTGAATCATTTGA+AGG | 0.554622 | 5.4:-15690885 | MS.gene72658:CDS |
| GGTATTATCTGGAGAAGCAA+AGG | 0.556103 | 5.4:-15687935 | MS.gene72658:CDS |
| GGAGGCCATCTTCGTCAGAC+CGG | 0.556644 | 5.4:-15691125 | MS.gene72658:CDS |
| AAACGGAGTTCAGAGGTTCT+GGG | 0.556723 | 5.4:-15689575 | MS.gene72658:CDS |
| AACCTAGAAGTTTGGAAGCA+GGG | 0.559255 | 5.4:+15689869 | None:intergenic |
| ATCAGGTCCTTGCAATACCC+TGG | 0.559752 | 5.4:-15694186 | MS.gene72658:CDS |
| AGCTCAAGTAACAAGTGGCA+TGG | 0.560858 | 5.4:-15686600 | MS.gene72658:CDS |
| TGTTCCTTTAGTCAACTTGG+AGG | 0.562952 | 5.4:-15686664 | MS.gene72658:CDS |
| TTAAGGTGCGATTATGTTTG+GGG | 0.564590 | 5.4:-15686858 | MS.gene72658:intron |
| GTTATGATGTAGAGATTGGT+GGG | 0.564818 | 5.4:-15690842 | MS.gene72658:CDS |
| ATGACGCTGCTGAGATTGAA+GGG | 0.566125 | 5.4:-15689538 | MS.gene72658:CDS |
| AATAACCGGTCTGACGAAGA+TGG | 0.566285 | 5.4:+15691120 | None:intergenic |
| GATGATGATAGAAAGGAAGT+AGG | 0.569154 | 5.4:-15695403 | MS.gene72658:CDS |
| TTTGAAGGTTATGAGTGCCA+TGG | 0.569684 | 5.4:-15690870 | MS.gene72658:CDS |
| TTTGGAGTAAGCAAAACCTA+GGG | 0.572609 | 5.4:-15690713 | MS.gene72658:CDS |
| AGTGCCTCCAAGTTGACTAA+AGG | 0.574131 | 5.4:+15686660 | None:intergenic |
| TTGCAACTTCTACACAGGGA+CGG | 0.575314 | 5.4:+15690655 | None:intergenic |
| AAGAGTTGCTCTACACAAAG+AGG | 0.577971 | 5.4:-15690798 | MS.gene72658:CDS |
| GACAACATAACTGGAGTTTG+AGG | 0.579541 | 5.4:+15688994 | None:intergenic |
| GGAGGAAACAAATCCAATCC+AGG | 0.580368 | 5.4:-15694636 | MS.gene72658:intron |
| TTGATTGAAGTACCTTATTG+GGG | 0.583424 | 5.4:+15694488 | None:intergenic |
| CTTCTACTTCTATGAAATGG+AGG | 0.585489 | 5.4:+15689958 | None:intergenic |
| AGGTCCCTTGCAAACGAACT+GGG | 0.587913 | 5.4:+15693888 | None:intergenic |
| AATGACAATCGCTCTTGTTG+CGG | 0.589049 | 5.4:-15687390 | MS.gene72658:intron |
| GAGGTGGAATTACTCGAGAT+GGG | 0.591799 | 5.4:-15696474 | MS.gene72658:CDS |
| TAGAAGTTTGGAAGCAGGGT+CGG | 0.592890 | 5.4:+15689873 | None:intergenic |
| ATTTCTTACTGATAGTATCA+GGG | 0.593444 | 5.4:+15688768 | None:intergenic |
| TTTGTTTCCTCCGAACTGGT+TGG | 0.594493 | 5.4:+15694647 | None:intergenic |
| TTGACTAAAGGAACAGTGGT+TGG | 0.596025 | 5.4:+15686672 | None:intergenic |
| CGAGATGGGCTGGAATTGGT+GGG | 0.596864 | 5.4:-15696460 | MS.gene72658:CDS |
| GAGCATCTAGATGCAGTACA+AGG | 0.602335 | 5.4:-15690326 | MS.gene72658:CDS |
| AACGGAGTTCAGAGGTTCTG+GGG | 0.602590 | 5.4:-15689574 | MS.gene72658:CDS |
| ATAATCTCACCTGGAAACAG+AGG | 0.602946 | 5.4:+15686780 | None:intergenic |
| ACTTCAGTTATGACATGTCA+AGG | 0.604592 | 5.4:-15690266 | MS.gene72658:CDS |
| ATGAATCACCTTCATATCCA+GGG | 0.605531 | 5.4:+15694169 | None:intergenic |
| TTCAAGATATATGCAGGGCA+TGG | 0.607221 | 5.4:+15693766 | None:intergenic |
| TGGAGACGAAAGAATCAGAC+TGG | 0.608130 | 5.4:-15688047 | MS.gene72658:CDS |
| GACCTGATCAATAACAGCAA+GGG | 0.609846 | 5.4:+15688679 | None:intergenic |
| CTCAAAACATATAAAGAGCG+GGG | 0.609938 | 5.4:+15693733 | None:intergenic |
| GGTTTGAGCATCGTAACTCG+TGG | 0.611142 | 5.4:-15693834 | MS.gene72658:CDS |
| TGCCAAAGTTCTTCATCAGG+TGG | 0.611303 | 5.4:-15688735 | MS.gene72658:CDS |
| ACTAGAAGGGAGCATTCTGA+TGG | 0.612665 | 5.4:+15686824 | None:intergenic |
| CTGTTGATTACTTACATCGA+GGG | 0.615245 | 5.4:-15691178 | MS.gene72658:CDS |
| CTGAATACCATGAATCTAGA+GGG | 0.615850 | 5.4:+15690568 | None:intergenic |
| CGACTTCACAAGAATCGTTG+AGG | 0.618882 | 5.4:-15696941 | MS.gene72658:CDS |
| CTACATCATAACCGAACCCA+TGG | 0.620434 | 5.4:+15690853 | None:intergenic |
| GCTGAATACCATGAATCTAG+AGG | 0.621627 | 5.4:+15690567 | None:intergenic |
| TAATCTCACCTGGAAACAGA+GGG | 0.622362 | 5.4:+15686781 | None:intergenic |
| TGTCTGAAGTGGAAAAGAAG+AGG | 0.623855 | 5.4:+15691252 | None:intergenic |
| CAATCAAGAAATGTGTGATG+CGG | 0.624369 | 5.4:-15694468 | MS.gene72658:CDS |
| TTTAGGAGTCGTACAACCGA+GGG | 0.628386 | 5.4:+15687072 | None:intergenic |
| AGCTCAAAACATATAAAGAG+CGG | 0.628689 | 5.4:+15693731 | None:intergenic |
| TCAACTACTTCATAGTTACA+GGG | 0.628877 | 5.4:-15687772 | MS.gene72658:CDS |
| CTAAATTCAAAATCGTGCAG+AGG | 0.632682 | 5.4:-15687054 | MS.gene72658:intron |
| TCATTGTGCAGTGTGCTCAT+GGG | 0.633237 | 5.4:-15686698 | MS.gene72658:intron |
| CATTTCATAGAAGTAGAAGG+AGG | 0.633795 | 5.4:-15689954 | MS.gene72658:CDS |
| TTCATATCCAGGGTATTGCA+AGG | 0.634101 | 5.4:+15694179 | None:intergenic |
| CAATGCAGTAGCTCGAGATG+AGG | 0.637844 | 5.4:+15690473 | None:intergenic |
| TAATGTGAAACGGAGTTCAG+AGG | 0.639049 | 5.4:-15689582 | MS.gene72658:CDS |
| GAGTAAGCAAAACCTAGGGG+AGG | 0.644151 | 5.4:-15690709 | MS.gene72658:CDS |
| ACAAGAGCTCAAGTAACAAG+TGG | 0.645352 | 5.4:-15686605 | MS.gene72658:CDS |
| TGACAATCATCAGTGCTACA+GGG | 0.650522 | 5.4:-15691216 | MS.gene72658:CDS |
| AGGGAACAGATTAAAGATTG+GGG | 0.650828 | 5.4:-15687753 | MS.gene72658:CDS |
| ATTTAGGAGTCGTACAACCG+AGG | 0.651899 | 5.4:+15687071 | None:intergenic |
| GACCTCCACCCCAAAGCCAG+TGG | 0.655193 | 5.4:+15694268 | None:intergenic |
| TAATGACAAGAACTTATGGA+CGG | 0.657018 | 5.4:-15695570 | MS.gene72658:CDS |
| TTAAATTCAAGATATATGCA+GGG | 0.659067 | 5.4:+15693761 | None:intergenic |
| AGACCTGATCAATAACAGCA+AGG | 0.659668 | 5.4:+15688678 | None:intergenic |
| AGGAGTCGTACAACCGAGGG+TGG | 0.660860 | 5.4:+15687075 | None:intergenic |
| CAACAAAATGATCCATTCAG+GGG | 0.663093 | 5.4:-15689497 | MS.gene72658:intron |
| GAGATTATGGGACTAAATGG+AGG | 0.664788 | 5.4:-15689026 | MS.gene72658:CDS |
| AAAGAGAAACAAAATAAGGG+AGG | 0.666982 | 5.4:-15691143 | MS.gene72658:CDS |
| CCAGTGGTTACAAGTGACAG+TGG | 0.669527 | 5.4:+15694284 | None:intergenic |
| TCTACAACCAACCAGTTCGG+AGG | 0.673318 | 5.4:-15694654 | MS.gene72658:CDS |
| ACGGAGTTCAGAGGTTCTGG+GGG | 0.673977 | 5.4:-15689573 | MS.gene72658:CDS |
| CTTCAGCTAGCTGATACTGA+CGG | 0.676315 | 5.4:-15687111 | MS.gene72658:intron |
| CAAGTTGACTAAAGGAACAG+TGG | 0.677062 | 5.4:+15686668 | None:intergenic |
| TGGGAGAGAAGCATAGCTTG+TGG | 0.677167 | 5.4:-15691690 | MS.gene72658:intron |
| AATGCTACGAGTGAAAACTT+CGG | 0.679690 | 5.4:-15695643 | MS.gene72658:CDS |
| TCTGTTGATTACTTACATCG+AGG | 0.679989 | 5.4:-15691179 | MS.gene72658:CDS |
| TCAAAACATATAAAGAGCGG+GGG | 0.683425 | 5.4:+15693734 | None:intergenic |
| GAAGAACTTTGGCATCCTCG+AGG | 0.706267 | 5.4:+15688744 | None:intergenic |
| TATGCGAGAAGTTCTTCACG+TGG | 0.708464 | 5.4:-15690936 | MS.gene72658:CDS |
| TCGATGTAAGTAATCAACAG+AGG | 0.724205 | 5.4:+15691181 | None:intergenic |
| TTGGGGCTAAGAGCGTCAGG+AGG | 0.725154 | 5.4:+15689731 | None:intergenic |
| TTGGAGTAAGCAAAACCTAG+GGG | 0.729433 | 5.4:-15690712 | MS.gene72658:CDS |
| CAATCATCAGTGCTACAGGG+AGG | 0.729888 | 5.4:-15691213 | MS.gene72658:CDS |
| ATGGTAGCTAAAAGACCCAG+CGG | 0.738543 | 5.4:-15689904 | MS.gene72658:CDS |
| CCTTTGTAGCGCGAGCATCG+AGG | 0.750669 | 5.4:+15696902 | None:intergenic |
| TATCAGTAAGAAATGCCTCG+AGG | 0.751021 | 5.4:-15688759 | MS.gene72658:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 15686536 | 15697028 | 15686536 | ID=MS.gene72658 |
| chr5.4 | mRNA | 15686536 | 15697028 | 15686536 | ID=MS.gene72658.t1;Parent=MS.gene72658 |
| chr5.4 | exon | 15696903 | 15697028 | 15696903 | ID=MS.gene72658.t1.exon1;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15696903 | 15697028 | 15696903 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15696758 | 15696809 | 15696758 | ID=MS.gene72658.t1.exon2;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15696758 | 15696809 | 15696758 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15696449 | 15696496 | 15696449 | ID=MS.gene72658.t1.exon3;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15696449 | 15696496 | 15696449 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15695543 | 15695697 | 15695543 | ID=MS.gene72658.t1.exon4;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15695543 | 15695697 | 15695543 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15695395 | 15695452 | 15695395 | ID=MS.gene72658.t1.exon5;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15695395 | 15695452 | 15695395 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15694637 | 15694694 | 15694637 | ID=MS.gene72658.t1.exon6;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15694637 | 15694694 | 15694637 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15694418 | 15694510 | 15694418 | ID=MS.gene72658.t1.exon7;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15694418 | 15694510 | 15694418 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15694178 | 15694343 | 15694178 | ID=MS.gene72658.t1.exon8;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15694178 | 15694343 | 15694178 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15694043 | 15694058 | 15694043 | ID=MS.gene72658.t1.exon9;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15694043 | 15694058 | 15694043 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15693699 | 15693919 | 15693699 | ID=MS.gene72658.t1.exon10;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15693699 | 15693919 | 15693699 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15691691 | 15691760 | 15691691 | ID=MS.gene72658.t1.exon11;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15691691 | 15691760 | 15691691 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15691518 | 15691598 | 15691518 | ID=MS.gene72658.t1.exon12;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15691518 | 15691598 | 15691518 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15689872 | 15691317 | 15689872 | ID=MS.gene72658.t1.exon13;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15689872 | 15691317 | 15689872 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15689704 | 15689782 | 15689704 | ID=MS.gene72658.t1.exon14;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15689704 | 15689782 | 15689704 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15689498 | 15689607 | 15689498 | ID=MS.gene72658.t1.exon15;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15689498 | 15689607 | 15689498 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15689005 | 15689078 | 15689005 | ID=MS.gene72658.t1.exon16;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15689005 | 15689078 | 15689005 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15688682 | 15688876 | 15688682 | ID=MS.gene72658.t1.exon17;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15688682 | 15688876 | 15688682 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15688030 | 15688074 | 15688030 | ID=MS.gene72658.t1.exon18;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15688030 | 15688074 | 15688030 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15687903 | 15687956 | 15687903 | ID=MS.gene72658.t1.exon19;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15687903 | 15687956 | 15687903 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15687708 | 15687814 | 15687708 | ID=MS.gene72658.t1.exon20;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15687708 | 15687814 | 15687708 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15687391 | 15687439 | 15687391 | ID=MS.gene72658.t1.exon21;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15687391 | 15687439 | 15687391 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15687228 | 15687287 | 15687228 | ID=MS.gene72658.t1.exon22;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15687228 | 15687287 | 15687228 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15687055 | 15687127 | 15687055 | ID=MS.gene72658.t1.exon23;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15687055 | 15687127 | 15687055 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15686790 | 15686875 | 15686790 | ID=MS.gene72658.t1.exon24;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15686790 | 15686875 | 15686790 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| chr5.4 | exon | 15686536 | 15686709 | 15686536 | ID=MS.gene72658.t1.exon25;Parent=MS.gene72658.t1 |
| chr5.4 | CDS | 15686536 | 15686709 | 15686536 | ID=cds.MS.gene72658.t1;Parent=MS.gene72658.t1 |
| Gene Sequence |
| Protein sequence |
