Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73017.t1 | XP_013457586.1 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 1.40E-30 | 141.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73017.t1 | Q13395 | 49.3 | 67 | 31 | 1 | 8 | 74 | 1443 | 1506 | 4.1e-07 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73017.t1 | A0A072UPJ3 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 9.9e-31 | 141.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene73017 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene73017 | 0.806667 | 7.36E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene73017 | 0.820159 | 7.95E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene73017 | 0.820256 | 7.55E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene73017 | 0.809309 | 2.02E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene73017 | 0.812965 | 3.25E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene73017 | 0.815976 | 7.01E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene73017 | 0.81837 | 2.03E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene73017 | 0.809612 | 1.74E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene73017 | 0.80936 | 1.97E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene73017 | 0.800578 | 1.35E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73017.t1 | MTR_4g099300 | 98.684 | 76 | 1 | 0 | 1 | 76 | 1655 | 1730 | 6.91e-44 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73017.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1641 | 1714 | 5.60e-28 | 105 |
| MS.gene73017.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1642 | 1715 | 5.60e-28 | 105 |
| MS.gene73017.t1 | AT4G17610 | 67.568 | 74 | 24 | 0 | 3 | 76 | 1649 | 1722 | 6.00e-28 | 105 |
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCAGGTGTTTAAAGCATC+AGG | 0.355325 | 8.1:-22993059 | MS.gene73017:intron |
| AGAAAGGGATGATCTTCTTC+TGG | 0.363171 | 8.1:-22993302 | MS.gene73017:CDS |
| CATACCCAATCTTGCTGGTT+TGG | 0.366415 | 8.1:-22993191 | MS.gene73017:CDS |
| GACCGCATACCCAATCTTGC+TGG | 0.373769 | 8.1:-22993196 | MS.gene73017:CDS |
| ACAAACAGTTCCAACTAATC+AGG | 0.386510 | 8.1:-22993004 | None:intergenic |
| ATCTTCTTGATTGCAAAACT+TGG | 0.400232 | 8.1:+22993278 | None:intergenic |
| ATCATGAAAACCTGATTAGT+TGG | 0.403453 | 8.1:+22992994 | None:intergenic |
| ACGTGCCAAACCAGCAAGAT+TGG | 0.437473 | 8.1:+22993186 | None:intergenic |
| TCAACATGACAGAGATAGAA+AGG | 0.467247 | 8.1:-22993318 | None:intergenic |
| CGTGCCAAACCAGCAAGATT+GGG | 0.501211 | 8.1:+22993187 | None:intergenic |
| ATCGTCTCTAGATCAACAAA+AGG | 0.502025 | 8.1:-22993257 | MS.gene73017:CDS |
| GGTGTTTAAAGCATCAGGGC+TGG | 0.579563 | 8.1:-22993054 | MS.gene73017:CDS |
| GGTCAATAAGTGATGCTACA+AGG | 0.590453 | 8.1:+22993215 | None:intergenic |
| CAACATGACAGAGATAGAAA+GGG | 0.597624 | 8.1:-22993317 | None:intergenic |
| AACCAGCAAGATTGGGTATG+CGG | 0.598882 | 8.1:+22993194 | None:intergenic |
| TGCAGGTGTTTAAAGCATCA+GGG | 0.599927 | 8.1:-22993058 | MS.gene73017:intron |
| TGGTTTGGCACGTACGTGTG+AGG | 0.670114 | 8.1:-22993176 | MS.gene73017:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATATAGAAAAGAT+TGG | + | chr8.1:22993193-22993212 | None:intergenic | 10.0% |
| !! | ATAATAATATAGAAAAGATT+GGG | + | chr8.1:22993192-22993211 | None:intergenic | 10.0% |
| ATCTTCTTGATTGCAAAACT+TGG | + | chr8.1:22993043-22993062 | None:intergenic | 30.0% | |
| TTCACTTCTATTGTTAATGC+AGG | - | chr8.1:22993243-22993262 | MS.gene73017:CDS | 30.0% | |
| ! | AAGTTTGTTTGGAGAGATAA+AGG | + | chr8.1:22993225-22993244 | None:intergenic | 30.0% |
| ! | CAATAGAAGTGAAGTTTGTT+TGG | + | chr8.1:22993236-22993255 | None:intergenic | 30.0% |
| ATCGTCTCTAGATCAACAAA+AGG | - | chr8.1:22993061-22993080 | MS.gene73017:intron | 35.0% | |
| AGAAAGGGATGATCTTCTTC+TGG | - | chr8.1:22993016-22993035 | MS.gene73017:CDS | 40.0% | |
| GGTCAATAAGTGATGCTACA+AGG | + | chr8.1:22993106-22993125 | None:intergenic | 40.0% | |
| ! | ATGCAGGTGTTTAAAGCATC+AGG | - | chr8.1:22993259-22993278 | MS.gene73017:CDS | 40.0% |
| ! | TGCAGGTGTTTAAAGCATCA+GGG | - | chr8.1:22993260-22993279 | MS.gene73017:CDS | 40.0% |
| AACCAGCAAGATTGGGTATG+CGG | + | chr8.1:22993127-22993146 | None:intergenic | 45.0% | |
| ! | CATACCCAATCTTGCTGGTT+TGG | - | chr8.1:22993127-22993146 | MS.gene73017:intron | 45.0% |
| ACGTGCCAAACCAGCAAGAT+TGG | + | chr8.1:22993135-22993154 | None:intergenic | 50.0% | |
| CGTGCCAAACCAGCAAGATT+GGG | + | chr8.1:22993134-22993153 | None:intergenic | 50.0% | |
| ! | GGTGTTTAAAGCATCAGGGC+TGG | - | chr8.1:22993264-22993283 | MS.gene73017:CDS | 50.0% |
| GACCGCATACCCAATCTTGC+TGG | - | chr8.1:22993122-22993141 | MS.gene73017:intron | 55.0% | |
| ! | TGGTTTGGCACGTACGTGTG+AGG | - | chr8.1:22993142-22993161 | MS.gene73017:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 22993005 | 22993335 | 22993005 | ID=MS.gene73017 |
| chr8.1 | mRNA | 22993005 | 22993335 | 22993005 | ID=MS.gene73017.t1;Parent=MS.gene73017 |
| chr8.1 | exon | 22993177 | 22993335 | 22993177 | ID=MS.gene73017.t1.exon1;Parent=MS.gene73017.t1 |
| chr8.1 | CDS | 22993177 | 22993335 | 22993177 | ID=cds.MS.gene73017.t1;Parent=MS.gene73017.t1 |
| chr8.1 | exon | 22993005 | 22993075 | 22993005 | ID=MS.gene73017.t1.exon2;Parent=MS.gene73017.t1 |
| chr8.1 | CDS | 22993005 | 22993075 | 22993005 | ID=cds.MS.gene73017.t1;Parent=MS.gene73017.t1 |
| Gene Sequence |
| Protein sequence |
