AlfalfaGEDB | MS.gene73044

M. sativa cultivar XinJiangDaYe / MS.gene73044

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73044.t1	RHN65932.1	98.1	107	2	0	1	107	80	186	4.00E-52	213.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73044.t1	F4J4N3	51.4	107	52	0	1	107	165	271	1.2e-25	117.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73044.t1	A0A396IK46	98.1	107	2	0	1	107	80	186	2.9e-52	213.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene73044	0.806756	7.05E-50	-1.69E-46
MS.gene050807	MS.gene73044	0.832854	7.45E-56	-1.69E-46
MS.gene050808	MS.gene73044	0.81537	9.57E-52	-1.69E-46
MS.gene051719	MS.gene73044	0.847085	1.45E-59	-1.69E-46
MS.gene051720	MS.gene73044	0.823685	1.21E-53	-1.69E-46
MS.gene052133	MS.gene73044	0.806723	7.16E-50	-1.69E-46
MS.gene053581	MS.gene73044	0.821577	3.75E-53	-1.69E-46
MS.gene055465	MS.gene73044	0.808968	2.39E-50	-1.69E-46
MS.gene055466	MS.gene73044	0.813364	2.66E-51	-1.69E-46
MS.gene056859	MS.gene73044	0.810359	1.20E-50	-1.69E-46
MS.gene060519	MS.gene73044	0.809154	2.18E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73044.t1	MTR_4g078885	65.421	107	37	0	1	107	78	184	1.13e-47	150
MS.gene73044.t1	MTR_3g034030	65.421	107	37	0	1	107	78	184	7.90e-47	149
MS.gene73044.t1	MTR_1g056370	55.556	108	47	1	1	107	78	185	1.65e-35	120
MS.gene73044.t1	MTR_4g013330	50.467	107	53	0	1	107	92	198	6.07e-33	114
MS.gene73044.t1	MTR_4g013350	50.467	107	53	0	1	107	90	196	1.90e-32	112
MS.gene73044.t1	MTR_7g070390	47.664	107	56	0	1	107	85	191	2.21e-32	112
MS.gene73044.t1	MTR_4g013345	49.533	107	54	0	1	107	89	195	4.50e-32	111
MS.gene73044.t1	MTR_1g054525	51.402	107	50	2	1	107	87	191	2.30e-31	109
MS.gene73044.t1	MTR_4g013325	48.598	107	55	0	1	107	92	198	2.04e-30	107
MS.gene73044.t1	MTR_4g013335	49.533	107	54	0	1	107	89	195	5.00e-30	106
MS.gene73044.t1	MTR_4g073950	43.925	107	60	0	1	107	85	191	1.71e-29	104
MS.gene73044.t1	MTR_4g013320	46.729	107	57	0	1	107	92	198	3.82e-29	104
MS.gene73044.t1	MTR_7g093820	51.402	107	51	1	2	107	87	193	4.38e-28	101
MS.gene73044.t1	MTR_7g093790	49.533	107	53	1	2	107	84	190	6.41e-28	100
MS.gene73044.t1	MTR_4g013385	42.991	107	61	0	1	107	83	189	1.10e-27	100
MS.gene73044.t1	MTR_0433s0040	42.991	107	61	0	1	107	83	189	1.10e-27	100
MS.gene73044.t1	MTR_7g093850	52.336	107	50	1	2	107	87	193	2.47e-27	99.4
MS.gene73044.t1	MTR_4g013770	44.860	107	59	0	1	107	84	190	2.73e-27	99.4
MS.gene73044.t1	MTR_7g093830	49.533	107	53	1	2	107	86	192	4.95e-26	95.9
MS.gene73044.t1	MTR_4g013310	46.667	105	54	1	1	105	92	194	5.80e-26	95.9
MS.gene73044.t1	MTR_5g096120	42.991	107	60	1	2	107	82	188	6.70e-22	85.5
MS.gene73044.t1	MTR_7g093870	41.441	111	60	3	1	107	83	192	7.43e-20	80.5
MS.gene73044.t1	MTR_3g105640	38.318	107	65	1	2	107	81	187	1.09e-19	79.7
MS.gene73044.t1	MTR_4g013355	37.383	107	57	1	1	107	82	178	4.82e-19	77.8
MS.gene73044.t1	MTR_3g105630	36.449	107	67	1	2	107	82	188	1.10e-17	74.3
MS.gene73044.t1	MTR_4g049550	35.484	93	60	0	14	106	84	176	6.57e-15	67.0
MS.gene73044.t1	MTR_4g122110	32.710	107	72	0	1	107	73	179	1.24e-14	66.2
MS.gene73044.t1	MTR_4g049570	35.484	93	60	0	14	106	84	176	4.31e-14	64.7
MS.gene73044.t1	MTR_4g122130	36.782	87	55	0	1	87	73	159	3.16e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73044.t1	AT3G58090	51.402	107	52	0	1	107	165	271	3.75e-33	116
MS.gene73044.t1	AT1G65870	52.336	107	51	0	1	107	83	189	1.82e-32	112
MS.gene73044.t1	AT5G42500	49.533	107	52	1	1	107	81	185	4.44e-31	108
MS.gene73044.t1	AT2G21100	42.991	107	61	0	1	107	81	187	2.49e-29	104
MS.gene73044.t1	AT2G21100	42.991	107	61	0	1	107	81	187	2.49e-29	104
MS.gene73044.t1	AT1G22900	46.729	107	55	1	1	107	89	193	3.85e-28	101
MS.gene73044.t1	AT5G42510	46.729	107	55	1	1	107	78	182	1.03e-27	100
MS.gene73044.t1	AT3G13650	42.991	107	61	0	1	107	79	185	1.05e-27	100
MS.gene73044.t1	AT1G58170	43.925	107	60	0	1	107	78	184	1.17e-26	97.4
MS.gene73044.t1	AT1G55210	43.925	107	60	0	1	107	80	186	2.06e-26	97.1
MS.gene73044.t1	AT1G55210	43.925	107	60	0	1	107	80	186	2.06e-26	97.1
MS.gene73044.t1	AT3G13660	42.056	107	62	0	1	107	18	124	2.62e-26	94.7
MS.gene73044.t1	AT5G49040	45.370	108	57	2	1	107	84	190	3.07e-26	96.7
MS.gene73044.t1	AT3G13662	49.425	87	44	0	1	87	78	164	1.92e-24	91.7
MS.gene73044.t1	AT5G49030	51.724	87	42	0	1	87	84	170	4.54e-24	95.5
MS.gene73044.t1	AT4G38700	42.342	111	60	2	1	107	79	189	2.47e-19	79.0
MS.gene73044.t1	AT2G21110	33.962	106	70	0	2	107	80	185	2.56e-17	73.6
MS.gene73044.t1	AT4G11210	35.238	105	66	2	1	105	77	179	7.86e-13	61.6

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCTACTGCCAAGAGTGTT+TGG	0.323543	3.2:+11622690	MS.gene73044:CDS
TTGGCGGCACCGGCGTGTTT+AGG	0.334517	3.2:+11622654	MS.gene73044:CDS
CTGCCTATTGTTGGCGGCAC+CGG	0.371881	3.2:+11622644	MS.gene73044:CDS
GGTAGAGCTCAAGGGATGTT+TGG	0.378489	3.2:+11622497	MS.gene73044:CDS
CCGGCGTGTTTAGGTTTGCT+AGG	0.384387	3.2:+11622663	MS.gene73044:CDS
ACGCCGGTGCCGCCAACAAT+AGG	0.397084	3.2:-11622647	None:intergenic
TCTCTTGTTCTTCAGTTAAG+AGG	0.414535	3.2:-11622457	None:intergenic
GGCAGTACTCTTAGTATGCT+TGG	0.427011	3.2:+11622590	MS.gene73044:CDS
GCAGTTCTCTAACAGTATCT+TGG	0.438017	3.2:-11622625	None:intergenic
TGCTACTGCCAAGAGTGTTT+GGG	0.441160	3.2:+11622691	MS.gene73044:CDS
CGGCGTGTTTAGGTTTGCTA+GGG	0.449156	3.2:+11622664	MS.gene73044:CDS
ATCCCTTGAGCTCTACCAAC+AGG	0.451335	3.2:-11622491	None:intergenic
ATTAATCTTGCTTTCACTGA+AGG	0.457487	3.2:+11622557	MS.gene73044:CDS
GTTAGAGAACTGCCTATTGT+TGG	0.474526	3.2:+11622635	MS.gene73044:CDS
TGAAATCTCCCAAACACTCT+TGG	0.494245	3.2:-11622699	None:intergenic
TCTCTAACAGTATCTTGGAC+AGG	0.511075	3.2:-11622620	None:intergenic
AAACCTGTTGGTAGAGCTCA+AGG	0.516264	3.2:+11622488	MS.gene73044:CDS
AGAGAACTGCCTATTGTTGG+CGG	0.541168	3.2:+11622638	MS.gene73044:CDS
GTACTCTTAGTATGCTTGGA+AGG	0.545035	3.2:+11622594	MS.gene73044:CDS
ATCTTTGCATGATCGTGGAA+TGG	0.559984	3.2:+11622526	MS.gene73044:CDS
GAGATAACCTCAAAACCTGT+TGG	0.572365	3.2:+11622476	MS.gene73044:CDS
TTAGCATCTTTGCATGATCG+TGG	0.581369	3.2:+11622521	MS.gene73044:CDS
GGCGTGTTTAGGTTTGCTAG+GGG	0.673525	3.2:+11622665	MS.gene73044:CDS
AACCTGTTGGTAGAGCTCAA+GGG	0.691584	3.2:+11622489	MS.gene73044:CDS
CCTAGCAAACCTAAACACGC+CGG	0.714305	3.2:-11622663	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTAATCTTGCTTTCACTGA+AGG	+	chr3.2:11622557-11622576	MS.gene73044:CDS	30.0%
	TCTCTTGTTCTTCAGTTAAG+AGG	-	chr3.2:11622460-11622479	None:intergenic	35.0%
	ATCTTTGCATGATCGTGGAA+TGG	+	chr3.2:11622526-11622545	MS.gene73044:CDS	40.0%
	GAGATAACCTCAAAACCTGT+TGG	+	chr3.2:11622476-11622495	MS.gene73044:CDS	40.0%
	GCAGTTCTCTAACAGTATCT+TGG	-	chr3.2:11622628-11622647	None:intergenic	40.0%
	GTACTCTTAGTATGCTTGGA+AGG	+	chr3.2:11622594-11622613	MS.gene73044:CDS	40.0%
	GTTAGAGAACTGCCTATTGT+TGG	+	chr3.2:11622635-11622654	MS.gene73044:CDS	40.0%
	TCTCTAACAGTATCTTGGAC+AGG	-	chr3.2:11622623-11622642	None:intergenic	40.0%
	TGAAATCTCCCAAACACTCT+TGG	-	chr3.2:11622702-11622721	None:intergenic	40.0%
	TTAGCATCTTTGCATGATCG+TGG	+	chr3.2:11622521-11622540	MS.gene73044:CDS	40.0%
!	TTCACTGAAGGTGATTTTGC+TGG	+	chr3.2:11622569-11622588	MS.gene73044:CDS	40.0%
	AGAGAACTGCCTATTGTTGG+CGG	+	chr3.2:11622638-11622657	MS.gene73044:CDS	45.0%
	GGCAGTACTCTTAGTATGCT+TGG	+	chr3.2:11622590-11622609	MS.gene73044:CDS	45.0%
!	AAACCTGTTGGTAGAGCTCA+AGG	+	chr3.2:11622488-11622507	MS.gene73044:CDS	45.0%
!	AACCTGTTGGTAGAGCTCAA+GGG	+	chr3.2:11622489-11622508	MS.gene73044:CDS	45.0%
!	AGCTCTACCAACAGGTTTTG+AGG	-	chr3.2:11622486-11622505	None:intergenic	45.0%
!!	ATGCTACTGCCAAGAGTGTT+TGG	+	chr3.2:11622690-11622709	MS.gene73044:CDS	45.0%
!!	TGCTACTGCCAAGAGTGTTT+GGG	+	chr3.2:11622691-11622710	MS.gene73044:CDS	45.0%
	ATCCCTTGAGCTCTACCAAC+AGG	-	chr3.2:11622494-11622513	None:intergenic	50.0%
	CCTAGCAAACCTAAACACGC+CGG	-	chr3.2:11622666-11622685	None:intergenic	50.0%
	GGTAGAGCTCAAGGGATGTT+TGG	+	chr3.2:11622497-11622516	MS.gene73044:CDS	50.0%
!!	CGGCGTGTTTAGGTTTGCTA+GGG	+	chr3.2:11622664-11622683	MS.gene73044:CDS	50.0%
!!	GGCGTGTTTAGGTTTGCTAG+GGG	+	chr3.2:11622665-11622684	MS.gene73044:CDS	50.0%
!	CCGGCGTGTTTAGGTTTGCT+AGG	+	chr3.2:11622663-11622682	MS.gene73044:CDS	55.0%
	CTGCCTATTGTTGGCGGCAC+CGG	+	chr3.2:11622644-11622663	MS.gene73044:CDS	60.0%
!!	ACGCCGGTGCCGCCAACAAT+AGG	-	chr3.2:11622650-11622669	None:intergenic	65.0%
!!	TTGGCGGCACCGGCGTGTTT+AGG	+	chr3.2:11622654-11622673	MS.gene73044:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	11622446	11622772	11622446	ID=MS.gene73044
chr3.2	mRNA	11622446	11622772	11622446	ID=MS.gene73044.t1;Parent=MS.gene73044
chr3.2	exon	11622446	11622772	11622446	ID=MS.gene73044.t1.exon1;Parent=MS.gene73044.t1
chr3.2	CDS	11622446	11622772	11622446	ID=cds.MS.gene73044.t1;Parent=MS.gene73044.t1

Gene Sequence	>MS.gene73044 ATGATGGACAACCTCTTAACTGAAGAACAAGAGATAACCTCAAAACCTGTTGGTAGAGCTCAAGGGATGTTTGGTTTAGCATCTTTGCATGATCGTGGAATGGTTATGTTAATTAATCTTGCTTTCACTGAAGGTGATTTTGCTGGCAGTACTCTTAGTATGCTTGGAAGGAATCCTGTCCAAGATACTGTTAGAGAACTGCCTATTGTTGGCGGCACCGGCGTGTTTAGGTTTGCTAGGGGATATGCTACTGCCAAGAGTGTTTGGGAGATTTCAACATCTGAGCATTTTGTTGTTGAGTATGATATCACTGTTTCTCATTCATAG
Protein sequence	>MS.gene73044.t1 MMDNLLTEEQEITSKPVGRAQGMFGLASLHDRGMVMLINLAFTEGDFAGSTLSMLGRNPVQDTVRELPIVGGTGVFRFARGYATAKSVWEISTSEHFVVEYDITVSHS