Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73468.t1 | XP_003592570.1 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 6.60E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73468.t1 | G7IAV0 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 4.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene73468 | 0.867967 | 9.26E-66 | -1.69E-46 |
| MS.gene051226 | MS.gene73468 | 0.869242 | 3.58E-66 | -1.69E-46 |
| MS.gene051837 | MS.gene73468 | 0.899228 | 2.44E-77 | -1.69E-46 |
| MS.gene057093 | MS.gene73468 | 0.869596 | 2.75E-66 | -1.69E-46 |
| MS.gene057432 | MS.gene73468 | 0.837258 | 5.78E-57 | -1.69E-46 |
| MS.gene06061 | MS.gene73468 | 0.878919 | 1.90E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73468.t1 | MTR_1g108640 | 98.844 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 9.58e-125 | 348 |
| MS.gene73468.t1 | MTR_2g105800 | 46.452 | 155 | 69 | 4 | 18 | 170 | 13 | 155 | 6.14e-37 | 125 |
| MS.gene73468.t1 | MTR_8g107420 | 33.708 | 178 | 98 | 4 | 1 | 171 | 1 | 165 | 5.44e-17 | 74.3 |
| MS.gene73468.t1 | MTR_4g068850 | 26.667 | 105 | 75 | 1 | 20 | 124 | 21 | 123 | 1.60e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73468.t1 | AT3G03280 | 41.111 | 180 | 81 | 6 | 1 | 170 | 1 | 165 | 5.80e-36 | 123 |
| MS.gene73468.t1 | AT5G17350 | 40.957 | 188 | 87 | 6 | 1 | 170 | 1 | 182 | 1.98e-32 | 114 |
Find 37 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATCAAAGAAACCTATTT+TGG | 0.162510 | 1.4:-83342983 | MS.gene73468:CDS |
| TTCCTTTATCTTGACCTAAT+TGG | 0.198618 | 1.4:+83342943 | None:intergenic |
| GCAGATGAAGAATTGGAATT+TGG | 0.234414 | 1.4:-83343249 | MS.gene73468:CDS |
| CGGCTGAACACGTGTCTTTC+CGG | 0.234431 | 1.4:+83343119 | None:intergenic |
| GAGTTGACTAAGAAATAATT+TGG | 0.256837 | 1.4:+83343312 | None:intergenic |
| TGGAATCAGGGTTTAGTTAT+AGG | 0.257374 | 1.4:-83343017 | MS.gene73468:CDS |
| AGTTTGGAAGGTGTGGAATC+AGG | 0.279397 | 1.4:-83343030 | MS.gene73468:CDS |
| TCATTTATAGTCTCCAAAAT+AGG | 0.290361 | 1.4:+83342970 | None:intergenic |
| TCTTGCTGCAGATGAAGAAT+TGG | 0.387242 | 1.4:-83343256 | MS.gene73468:CDS |
| AACCAATTAGGTCAAGATAA+AGG | 0.404180 | 1.4:-83342945 | None:intergenic |
| GTTTGGAAGGTGTGGAATCA+GGG | 0.418181 | 1.4:-83343029 | MS.gene73468:CDS |
| AATTGTTTCACTTCTCCTGT+TGG | 0.426516 | 1.4:+83343375 | None:intergenic |
| CTATAAATGAAGAACCAATT+AGG | 0.456816 | 1.4:-83342957 | MS.gene73468:CDS |
| ATGTGTTCCGAGGTTGAGTT+TGG | 0.464828 | 1.4:-83343046 | MS.gene73468:CDS |
| TGTTCAGCCGGATGAGAGTT+CGG | 0.467508 | 1.4:-83343106 | MS.gene73468:CDS |
| ACAGAATTGAGTCTTCTCAT+AGG | 0.502486 | 1.4:+83343207 | None:intergenic |
| CCTCATCAACGGTGGTGCTA+AGG | 0.505204 | 1.4:+83343425 | None:intergenic |
| CAGCACCGCCATGTCAGCTC+CGG | 0.507579 | 1.4:+83343179 | None:intergenic |
| GTTTCACTTCTCCTGTTGGA+AGG | 0.509220 | 1.4:+83343379 | None:intergenic |
| ACAAGAGTAATCCTTCCAAC+AGG | 0.510852 | 1.4:-83343390 | MS.gene73468:CDS |
| TGTGGTCACCGGAGCTGACA+TGG | 0.524219 | 1.4:-83343187 | MS.gene73468:CDS |
| CGGAAAGACACGTGTTCAGC+CGG | 0.528707 | 1.4:-83343118 | MS.gene73468:CDS |
| TTCACTTCCGAACTCTCATC+CGG | 0.533475 | 1.4:+83343099 | None:intergenic |
| GGATGAGAGTTCGGAAGTGA+AGG | 0.539299 | 1.4:-83343097 | MS.gene73468:CDS |
| CGGCGAATTCGGCTGCGAAG+CGG | 0.542084 | 1.4:-83343149 | MS.gene73468:CDS |
| GCTGCGAAGCGGTTACGTGC+CGG | 0.545063 | 1.4:-83343138 | MS.gene73468:CDS |
| AATTCTTCATCTGCAGCAAG+AGG | 0.547180 | 1.4:+83343258 | None:intergenic |
| AGACTCAATTCTGTGGTCAC+CGG | 0.558052 | 1.4:-83343198 | MS.gene73468:CDS |
| GAGGTTGAGTTTGGAAGGTG+TGG | 0.563066 | 1.4:-83343037 | MS.gene73468:CDS |
| CCTTAGCACCACCGTTGATG+AGG | 0.584450 | 1.4:-83343425 | MS.gene73468:CDS |
| GTTCCGAGGTTGAGTTTGGA+AGG | 0.607016 | 1.4:-83343042 | MS.gene73468:CDS |
| GATGAGAGTTCGGAAGTGAA+GGG | 0.620669 | 1.4:-83343096 | MS.gene73468:CDS |
| ACACCTTCCAAACTCAACCT+CGG | 0.627609 | 1.4:+83343039 | None:intergenic |
| TATGAGAAGACTCAATTCTG+TGG | 0.636561 | 1.4:-83343205 | MS.gene73468:CDS |
| TTTGCGTTCCTCATCAACGG+TGG | 0.639490 | 1.4:+83343417 | None:intergenic |
| ATCAAAATGAATGTGTTCCG+AGG | 0.670622 | 1.4:-83343056 | MS.gene73468:CDS |
| GGTCACCGGAGCTGACATGG+CGG | 0.787053 | 1.4:-83343184 | MS.gene73468:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTATAAATGAAGAACCAATT+AGG | - | chr1.4:83343438-83343457 | MS.gene73468:CDS | 25.0% |
| ! | GAGTTGACTAAGAAATAATT+TGG | + | chr1.4:83343086-83343105 | None:intergenic | 25.0% |
| ! | TCATTTATAGTCTCCAAAAT+AGG | + | chr1.4:83343428-83343447 | None:intergenic | 25.0% |
| !! | TAGATCAAAGAAACCTATTT+TGG | - | chr1.4:83343412-83343431 | MS.gene73468:CDS | 25.0% |
| AATTGTTTCACTTCTCCTGT+TGG | + | chr1.4:83343023-83343042 | None:intergenic | 35.0% | |
| ACAGAATTGAGTCTTCTCAT+AGG | + | chr1.4:83343191-83343210 | None:intergenic | 35.0% | |
| ATCAAAATGAATGTGTTCCG+AGG | - | chr1.4:83343339-83343358 | MS.gene73468:CDS | 35.0% | |
| GCAGATGAAGAATTGGAATT+TGG | - | chr1.4:83343146-83343165 | MS.gene73468:CDS | 35.0% | |
| TATGAGAAGACTCAATTCTG+TGG | - | chr1.4:83343190-83343209 | MS.gene73468:CDS | 35.0% | |
| TGGAATCAGGGTTTAGTTAT+AGG | - | chr1.4:83343378-83343397 | MS.gene73468:CDS | 35.0% | |
| AATTCTTCATCTGCAGCAAG+AGG | + | chr1.4:83343140-83343159 | None:intergenic | 40.0% | |
| ACAAGAGTAATCCTTCCAAC+AGG | - | chr1.4:83343005-83343024 | MS.gene73468:CDS | 40.0% | |
| TCTTGCTGCAGATGAAGAAT+TGG | - | chr1.4:83343139-83343158 | MS.gene73468:CDS | 40.0% | |
| ACACCTTCCAAACTCAACCT+CGG | + | chr1.4:83343359-83343378 | None:intergenic | 45.0% | |
| AGACTCAATTCTGTGGTCAC+CGG | - | chr1.4:83343197-83343216 | MS.gene73468:CDS | 45.0% | |
| ATGTGTTCCGAGGTTGAGTT+TGG | - | chr1.4:83343349-83343368 | MS.gene73468:CDS | 45.0% | |
| GATGAGAGTTCGGAAGTGAA+GGG | - | chr1.4:83343299-83343318 | MS.gene73468:CDS | 45.0% | |
| GTTTCACTTCTCCTGTTGGA+AGG | + | chr1.4:83343019-83343038 | None:intergenic | 45.0% | |
| TTCACTTCCGAACTCTCATC+CGG | + | chr1.4:83343299-83343318 | None:intergenic | 45.0% | |
| ! | AGTTTGGAAGGTGTGGAATC+AGG | - | chr1.4:83343365-83343384 | MS.gene73468:CDS | 45.0% |
| ! | GCTTTTGCGTTCCTCATCAA+CGG | + | chr1.4:83342984-83343003 | None:intergenic | 45.0% |
| ! | GTTTGGAAGGTGTGGAATCA+GGG | - | chr1.4:83343366-83343385 | MS.gene73468:CDS | 45.0% |
| GAGGTTGAGTTTGGAAGGTG+TGG | - | chr1.4:83343358-83343377 | MS.gene73468:CDS | 50.0% | |
| GGATGAGAGTTCGGAAGTGA+AGG | - | chr1.4:83343298-83343317 | MS.gene73468:CDS | 50.0% | |
| GTTCCGAGGTTGAGTTTGGA+AGG | - | chr1.4:83343353-83343372 | MS.gene73468:CDS | 50.0% | |
| TGTTCAGCCGGATGAGAGTT+CGG | - | chr1.4:83343289-83343308 | MS.gene73468:CDS | 50.0% | |
| TTTGCGTTCCTCATCAACGG+TGG | + | chr1.4:83342981-83343000 | None:intergenic | 50.0% | |
| !!! | GCTGTTTTTGGCGGCGAATT+CGG | - | chr1.4:83343235-83343254 | MS.gene73468:CDS | 50.0% |
| !!! | TGACATGGCGGTGCTGTTTT+TGG | - | chr1.4:83343223-83343242 | MS.gene73468:CDS | 50.0% |
| CCTTAGCACCACCGTTGATG+AGG | - | chr1.4:83342970-83342989 | MS.gene73468:CDS | 55.0% | |
| CGGAAAGACACGTGTTCAGC+CGG | - | chr1.4:83343277-83343296 | MS.gene73468:CDS | 55.0% | |
| CGGCTGAACACGTGTCTTTC+CGG | + | chr1.4:83343279-83343298 | None:intergenic | 55.0% | |
| ! | CCTCATCAACGGTGGTGCTA+AGG | + | chr1.4:83342973-83342992 | None:intergenic | 55.0% |
| !!! | CATGGCGGTGCTGTTTTTGG+CGG | - | chr1.4:83343226-83343245 | MS.gene73468:CDS | 55.0% |
| TGTGGTCACCGGAGCTGACA+TGG | - | chr1.4:83343208-83343227 | MS.gene73468:CDS | 60.0% | |
| CAGCACCGCCATGTCAGCTC+CGG | + | chr1.4:83343219-83343238 | None:intergenic | 65.0% | |
| CGGCGAATTCGGCTGCGAAG+CGG | - | chr1.4:83343246-83343265 | MS.gene73468:CDS | 65.0% | |
| GCTGCGAAGCGGTTACGTGC+CGG | - | chr1.4:83343257-83343276 | MS.gene73468:CDS | 65.0% | |
| GGTCACCGGAGCTGACATGG+CGG | - | chr1.4:83343211-83343230 | MS.gene73468:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 83342948 | 83343469 | 83342948 | ID=MS.gene73468 |
| chr1.4 | mRNA | 83342948 | 83343469 | 83342948 | ID=MS.gene73468.t1;Parent=MS.gene73468 |
| chr1.4 | exon | 83342948 | 83343469 | 83342948 | ID=MS.gene73468.t1.exon1;Parent=MS.gene73468.t1 |
| chr1.4 | CDS | 83342948 | 83343469 | 83342948 | ID=cds.MS.gene73468.t1;Parent=MS.gene73468.t1 |
| Gene Sequence |
| Protein sequence |
