AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73918

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73918.t1 MTR_5g059260 98.472 589 9 0 1 589 1 589 0.0 1213
MS.gene73918.t1 MTR_1g031590 55.121 537 215 6 26 559 33 546 0.0 597
MS.gene73918.t1 MTR_4g084570 56.942 497 196 4 68 559 64 547 0.0 595
MS.gene73918.t1 MTR_3g005340 55.030 507 210 5 72 569 57 554 0.0 565
MS.gene73918.t1 MTR_5g032100 41.080 426 214 11 159 559 88 501 2.03e-94 298
MS.gene73918.t1 MTR_2g016470 38.852 453 239 10 139 561 172 616 9.82e-94 300
MS.gene73918.t1 MTR_3g100310 40.141 426 218 10 159 559 102 515 1.91e-92 294
MS.gene73918.t1 MTR_3g086570 37.995 429 246 10 139 559 131 547 5.12e-91 291
MS.gene73918.t1 MTR_4g100810 44.034 352 181 6 158 509 109 444 2.34e-90 289
MS.gene73918.t1 MTR_5g064620 38.041 439 235 10 140 562 97 514 1.80e-88 283
MS.gene73918.t1 MTR_5g099010 43.288 365 189 6 160 523 122 469 2.73e-88 285
MS.gene73918.t1 MTR_3g115220 41.053 380 207 6 141 519 95 458 9.30e-88 283
MS.gene73918.t1 MTR_5g012770 43.820 356 165 9 158 509 125 449 1.49e-85 275
MS.gene73918.t1 MTR_3g099480 36.902 439 239 12 140 563 126 541 1.08e-83 272
MS.gene73918.t1 MTR_6g016015 38.849 417 226 11 158 564 116 513 1.42e-83 270
MS.gene73918.t1 MTR_4g110030 39.036 415 224 10 158 563 117 511 2.69e-82 267
MS.gene73918.t1 MTR_3g096020 38.072 415 228 7 157 559 189 586 9.00e-79 260
MS.gene73918.t1 MTR_2g059350 36.501 463 245 13 112 563 86 510 1.10e-78 258
MS.gene73918.t1 MTR_4g082310 36.650 412 231 10 158 559 98 489 1.17e-77 255
MS.gene73918.t1 MTR_5g042200 36.893 412 230 10 158 559 86 477 1.82e-77 254
MS.gene73918.t1 MTR_4g132590 37.168 452 241 9 140 559 52 492 4.77e-77 253
MS.gene73918.t1 MTR_4g132590 37.168 452 241 9 140 559 151 591 5.10e-76 253
MS.gene73918.t1 MTR_5g081690 36.471 425 235 12 158 571 122 522 8.00e-76 250
MS.gene73918.t1 MTR_4g125460 37.412 425 232 13 146 559 77 478 1.01e-75 249
MS.gene73918.t1 MTR_4g125460 37.412 425 232 13 146 559 216 617 1.83e-74 249
MS.gene73918.t1 MTR_4g019210 35.581 430 242 14 158 575 233 639 6.99e-72 243
MS.gene73918.t1 MTR_1g102030 35.597 427 217 15 158 559 89 482 6.91e-68 229
MS.gene73918.t1 MTR_3g086570 38.361 305 175 6 139 435 131 430 7.29e-67 224
MS.gene73918.t1 MTR_3g026650 32.494 437 246 8 139 559 153 556 2.54e-65 223
MS.gene73918.t1 MTR_4g023030 31.556 450 278 9 116 559 191 616 8.27e-61 213
MS.gene73918.t1 MTR_3g026650 33.053 357 196 6 139 479 153 482 2.14e-51 184
MS.gene73918.t1 MTR_4g010990 31.714 391 222 11 128 508 48 403 9.16e-48 173
MS.gene73918.t1 MTR_1g101990 35.000 300 166 10 267 559 1 278 6.37e-46 164
MS.gene73918.t1 MTR_4g010990 31.085 341 202 8 173 508 2 314 7.35e-41 152
MS.gene73918.t1 MTR_3g092590 32.895 304 188 6 158 452 91 387 1.49e-33 135
MS.gene73918.t1 MTR_3g092590 32.787 305 187 7 158 452 91 387 2.64e-33 135
MS.gene73918.t1 MTR_7g017330 28.496 379 236 13 107 470 55 413 4.69e-33 134
MS.gene73918.t1 MTR_3g026650 30.292 274 148 6 139 396 153 399 4.91e-29 119
MS.gene73918.t1 MTR_1g007390 31.270 307 187 8 158 448 91 389 7.64e-28 119
MS.gene73918.t1 MTR_4g008200 27.011 348 204 9 158 500 36 338 3.45e-25 107
MS.gene73918.t1 MTR_4g008200 27.011 348 204 9 158 500 87 389 7.74e-25 107
MS.gene73918.t1 MTR_2g038320 26.862 376 220 10 132 502 70 395 3.73e-24 105
MS.gene73918.t1 MTR_7g064210 33.500 200 106 4 278 466 27 210 4.12e-24 105
MS.gene73918.t1 MTR_1g101985 53.333 75 35 0 158 232 89 163 1.88e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73918.t1 AT1G38131 69.231 494 145 2 72 560 67 558 0.0 721
MS.gene73918.t1 AT1G38065 71.897 427 115 1 139 560 3 429 0.0 655
MS.gene73918.t1 AT1G38065 74.442 403 103 0 158 560 37 439 0.0 652
MS.gene73918.t1 AT2G01480 52.260 553 233 7 24 557 9 549 0.0 585
MS.gene73918.t1 AT1G35510 54.713 541 206 10 46 559 28 556 0.0 574
MS.gene73918.t1 AT1G14970 52.651 547 237 5 24 559 17 552 0.0 571
MS.gene73918.t1 AT2G01480 53.204 515 215 6 24 519 9 516 0.0 557
MS.gene73918.t1 AT1G14970 58.581 437 171 2 123 559 6 432 0.0 541
MS.gene73918.t1 AT1G14970 60.688 407 155 1 153 559 5 406 0.0 529
MS.gene73918.t1 AT4G38390 42.448 384 205 6 141 523 107 475 3.26e-96 305
MS.gene73918.t1 AT4G24530 39.686 446 229 12 139 559 86 516 7.98e-96 303
MS.gene73918.t1 AT5G65470 39.232 469 230 14 124 559 56 502 6.62e-95 300
MS.gene73918.t1 AT1G29200 41.628 430 216 9 158 559 71 493 1.04e-92 294
MS.gene73918.t1 AT1G29200 41.628 430 216 9 158 559 71 493 1.04e-92 294
MS.gene73918.t1 AT1G29200 41.628 430 216 9 158 559 187 609 1.15e-91 295
MS.gene73918.t1 AT1G29200 41.549 426 214 9 162 559 1 419 5.53e-91 287
MS.gene73918.t1 AT3G26370 37.529 429 248 10 139 559 139 555 2.14e-89 287
MS.gene73918.t1 AT4G16650 44.385 374 177 9 158 523 123 473 1.80e-87 282
MS.gene73918.t1 AT4G16650 44.385 374 177 9 158 523 123 473 1.80e-87 282
MS.gene73918.t1 AT1G11990 39.083 458 230 12 123 559 159 588 2.65e-87 283
MS.gene73918.t1 AT1G22460 39.762 420 216 12 159 562 162 560 1.71e-86 280
MS.gene73918.t1 AT1G62330 36.498 474 256 12 123 562 190 652 1.64e-85 280
MS.gene73918.t1 AT1G20550 42.577 357 185 6 160 513 112 451 6.19e-85 276
MS.gene73918.t1 AT1G76270 40.426 376 204 6 141 513 90 448 8.50e-85 275
MS.gene73918.t1 AT1G11990 36.546 498 238 13 116 559 137 610 9.61e-84 274
MS.gene73918.t1 AT1G14020 37.379 412 228 10 158 559 92 483 8.26e-82 265
MS.gene73918.t1 AT5G64600 37.443 438 230 13 140 559 103 514 4.66e-81 264
MS.gene73918.t1 AT2G03280 37.968 374 210 7 158 525 89 446 1.02e-76 251
MS.gene73918.t1 AT5G15740 37.226 411 233 10 158 561 107 499 1.33e-76 252
MS.gene73918.t1 AT5G01100 35.971 417 227 11 158 559 217 608 2.69e-75 252
MS.gene73918.t1 AT3G02250 37.772 368 208 7 158 519 107 459 5.40e-75 248
MS.gene73918.t1 AT1G04910 36.842 418 216 11 158 559 89 474 3.70e-74 246
MS.gene73918.t1 AT3G54100 35.990 414 228 12 158 559 223 611 6.77e-73 246
MS.gene73918.t1 AT2G37980 36.740 411 229 12 158 559 227 615 1.65e-72 244
MS.gene73918.t1 AT5G35570 34.461 473 261 14 114 568 211 652 4.54e-71 241
MS.gene73918.t1 AT2G03280 35.411 401 210 8 158 525 89 473 5.57e-71 237
MS.gene73918.t1 AT5G63390 34.279 423 258 6 139 559 144 548 1.07e-70 238
MS.gene73918.t1 AT2G44500 33.034 445 273 10 119 559 138 561 4.01e-63 218
MS.gene73918.t1 AT3G07900 32.759 406 247 7 160 561 191 574 2.61e-61 213
MS.gene73918.t1 AT3G26370 36.929 241 140 5 139 372 139 374 6.87e-48 172
MS.gene73918.t1 AT1G52630 30.707 368 222 10 126 489 45 383 7.68e-46 168
MS.gene73918.t1 AT1G52630 30.218 321 193 8 173 489 2 295 3.03e-38 144
MS.gene73918.t1 AT3G03810 30.352 369 232 9 109 464 42 398 4.02e-36 144
MS.gene73918.t1 AT3G03810 30.352 369 232 9 109 464 42 398 4.02e-36 144
MS.gene73918.t1 AT3G30300 31.210 314 188 11 158 456 101 401 9.43e-32 130
MS.gene73918.t1 AT3G30300 31.210 314 188 11 158 456 101 401 9.43e-32 130
MS.gene73918.t1 AT2G44500 32.824 262 158 7 139 396 157 404 3.08e-28 117
MS.gene73918.t1 AT1G51630 26.287 369 217 10 137 500 75 393 4.88e-24 105
MS.gene73918.t1 AT3G21190 26.513 347 204 9 159 500 92 392 1.41e-22 100

Find 146 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGCATCATGAACATTT+TGG 0.253855 5.2:+50376572 MS.gene73918:CDS
GGCGGTGGCGGAGGCGGAGG+TGG 0.258644 5.2:+50375729 MS.gene73918:CDS
CTCTTCCTGGTTCTCGTTAC+CGG 0.260601 5.2:+50375970 MS.gene73918:CDS
ACAACAAGTTTGCGTTTATC+AGG 0.270838 5.2:-50380326 None:intergenic
ACATCAATATATTTAGCTTC+TGG 0.271700 5.2:+50379475 MS.gene73918:CDS
TGTAATGCTGTGGCTGTTGC+TGG 0.286549 5.2:+50377214 MS.gene73918:CDS
ATATCATGCAGAAAAGTATT+TGG 0.315152 5.2:+50379504 MS.gene73918:CDS
TTTATCAGGAATAATGGTTT+TGG 0.315630 5.2:-50380312 None:intergenic
GAATTATCAGAATCAATTTC+AGG 0.320818 5.2:-50376020 None:intergenic
ATTGGACATACTCCTCTTCC+TGG 0.322538 5.2:+50375957 MS.gene73918:CDS
TTTGCGTTTATCAGGAATAA+TGG 0.323925 5.2:-50380318 None:intergenic
AGAACTTCCCGAGGCAATAA+TGG 0.326209 5.2:+50378350 MS.gene73918:CDS
GGCGGAGGCGGAGGTGGAGG+TGG 0.328837 5.2:+50375735 MS.gene73918:CDS
GTCATACCGCAGAGCGTTGC+TGG 0.339373 5.2:-50377098 None:intergenic
CATCAATATATTTAGCTTCT+GGG 0.347457 5.2:+50379476 MS.gene73918:CDS
TGATGAGCTTGCTCCTTCTA+TGG 0.353381 5.2:+50379582 MS.gene73918:CDS
TACATTTACGGAAACGGTTA+CGG 0.355604 5.2:+50375811 MS.gene73918:CDS
TGCTGTGCATCTTCGCTTTG+AGG 0.361326 5.2:+50378810 MS.gene73918:CDS
GGTGGCGGAGGCGGAGGTGG+AGG 0.368387 5.2:+50375732 MS.gene73918:CDS
AATGGCGGTGGCGGAGGCGG+AGG 0.369247 5.2:+50375726 MS.gene73918:CDS
AGCTCCATTTGCTAATCGTT+TGG 0.369656 5.2:+50378642 MS.gene73918:CDS
TTTCCGTTCACGTTCTTCAT+TGG 0.376601 5.2:+50375939 MS.gene73918:CDS
CGATCTCCACCGCGACATTC+CGG 0.377998 5.2:+50375783 MS.gene73918:CDS
GCCTCCGCCACCGCCATTCT+CGG 0.378440 5.2:-50375721 None:intergenic
CTGCCGGTGGTTTATCTCTC+CGG 0.379278 5.2:+50375894 MS.gene73918:CDS
TTCCTCCCAATTGGCAGCAT+TGG 0.382578 5.2:+50380187 MS.gene73918:CDS
ATCTGCACTAGCTGAGAATC+TGG 0.385628 5.2:+50378741 MS.gene73918:CDS
TGGAGGAAAGGCAGAGAAGT+TGG 0.390688 5.2:+50378986 MS.gene73918:CDS
AGACTCCTTGGTATAAAGAT+TGG 0.391718 5.2:-50379548 None:intergenic
CACCATAGAGAAATCTTCTA+TGG 0.392874 5.2:-50380283 None:intergenic
GAGAATGGCGGTGGCGGAGG+CGG 0.393770 5.2:+50375723 MS.gene73918:CDS
TGCTGTAGACTGTAGTTATC+AGG 0.403998 5.2:-50383150 None:intergenic
CACTGAGCATGTCATCCATC+TGG 0.414354 5.2:-50382987 None:intergenic
TCCTGTAGGCATCTACATTC+TGG 0.420118 5.2:-50383087 None:intergenic
GCCGAGAATGGCGGTGGCGG+AGG 0.424599 5.2:+50375720 MS.gene73918:CDS
GACTCCTTGGTATAAAGATT+GGG 0.435088 5.2:-50379547 None:intergenic
ATAGAAGGAGCAAGCTCATC+AGG 0.440455 5.2:-50379580 None:intergenic
TCAGTGTCCCCTGGCCTGAA+TGG 0.442293 5.2:+50377042 MS.gene73918:intron
GGCATCTACATTCTGGTAAA+GGG 0.445482 5.2:-50383080 None:intergenic
AAAGGCAGAGAAGTTGGAAA+TGG 0.445978 5.2:+50378992 MS.gene73918:CDS
TTCACTAACCTCAATGAAAG+AGG 0.447464 5.2:-50376042 None:intergenic
ATGTCTCTCCATTATTGCCT+CGG 0.452425 5.2:-50378358 None:intergenic
GGTGGAGATCGCGGTGGACT+CGG 0.455910 5.2:-50375771 None:intergenic
TGTTCATCCTATCTTGCAAA+AGG 0.458231 5.2:+50378452 MS.gene73918:CDS
CCATTGACTCGATAGAGGTC+AGG 0.459450 5.2:-50379074 None:intergenic
ATGGATTCAGCTCGTGAAAA+GGG 0.461884 5.2:+50379011 MS.gene73918:CDS
ATGAAGAACGTGAACGGAAA+CGG 0.461897 5.2:-50375936 None:intergenic
TCCTCCATCATATACACAAC+AGG 0.464616 5.2:-50378970 None:intergenic
ATAGGGTTATTCCTCCCAAT+TGG 0.466456 5.2:+50380178 MS.gene73918:intron
CTATGGCCCATTAGAAAATG+AGG 0.466547 5.2:-50380266 None:intergenic
GTATAACTAGAATTGCATTC+AGG 0.467924 5.2:-50377240 None:intergenic
GCGGCCGAGAATGGCGGTGG+CGG 0.467955 5.2:+50375717 MS.gene73918:CDS
TGTAGTCCAATGCTGCCAAT+TGG 0.470944 5.2:-50380193 None:intergenic
TGTCTCTCCATTATTGCCTC+GGG 0.474698 5.2:-50378357 None:intergenic
AAAGGAAAGTTCAAAAGAAA+AGG 0.475308 5.2:+50379038 MS.gene73918:CDS
TCTATAATTTCAGTGTCCCC+TGG 0.476470 5.2:+50377033 MS.gene73918:intron
GGCCATAGAAGATTTCTCTA+TGG 0.478214 5.2:+50380281 MS.gene73918:CDS
CCTGACCTCTATCGAGTCAA+TGG 0.479728 5.2:+50379074 MS.gene73918:CDS
AGGCATCTACATTCTGGTAA+AGG 0.481645 5.2:-50383081 None:intergenic
CAAGCAAATAAATACCTCTA+GGG 0.483434 5.2:-50379114 None:intergenic
AACGGTTACGCGGCCGAGAA+TGG 0.483832 5.2:+50375708 MS.gene73918:CDS
CTTCAGTGTGGAAATACAAA+AGG 0.485641 5.2:+50376513 MS.gene73918:CDS
AATGGATTCAGCTCGTGAAA+AGG 0.486427 5.2:+50379010 MS.gene73918:CDS
TTGCAGTTGGAGTGCTCTCA+AGG 0.490827 5.2:+50382963 MS.gene73918:intron
GAGAAGAGCTCGAGGACAGA+TGG 0.496882 5.2:+50378778 MS.gene73918:CDS
TAGGGACCATGATTCCTTTG+CGG 0.503683 5.2:-50383017 None:intergenic
CATCCTATCTTGCAAAAGGA+AGG 0.504394 5.2:+50378456 MS.gene73918:CDS
CGGACGAAGTCACTGTCGGC+GGG 0.506458 5.2:+50375834 MS.gene73918:CDS
CTAGCATCAGAGTTGAAGCT+TGG 0.507242 5.2:+50378403 MS.gene73918:CDS
TACGCGGCCGAGAATGGCGG+TGG 0.508173 5.2:+50375714 MS.gene73918:CDS
CATCTTAGAAATTGTATATG+TGG 0.509769 5.2:-50378679 None:intergenic
TCCTACACACATCAACAAAC+CGG 0.512142 5.2:-50375913 None:intergenic
TGGGATGATGCTACGTGGCA+TGG 0.512796 5.2:+50379438 MS.gene73918:CDS
TAGCATCAGAGTTGAAGCTT+GGG 0.516624 5.2:+50378404 MS.gene73918:CDS
AATACCTCTAGGGGAGTTAG+TGG 0.523241 5.2:-50379104 None:intergenic
GTAGTCCAATGCTGCCAATT+GGG 0.524395 5.2:-50380192 None:intergenic
TGATAATTCCTCTTTCATTG+AGG 0.528251 5.2:+50376034 MS.gene73918:CDS
GCTCCCGTCAGTTATTACCT+TGG 0.529344 5.2:+50378426 MS.gene73918:CDS
TTGGGAAACATCTTTATCAA+AGG 0.529494 5.2:-50379529 None:intergenic
GATCGAAAACCTCGTGACTC+CGG 0.529800 5.2:-50375989 None:intergenic
GGGATGATGCTACGTGGCAT+GGG 0.530350 5.2:+50379439 MS.gene73918:CDS
ACCAGAATGTAGATGCCTAC+AGG 0.530789 5.2:+50383086 MS.gene73918:CDS
CATAGAAGATTTCTCTATGG+TGG 0.537641 5.2:+50380284 MS.gene73918:CDS
TTGCTGGTTAAGACCACCAT+TGG 0.539892 5.2:-50377082 None:intergenic
AGGTGTTGCAAGAGACTCCT+TGG 0.542412 5.2:-50379560 None:intergenic
AAGTGACCGCAAAGGAATCA+TGG 0.542741 5.2:+50383011 MS.gene73918:CDS
ATTGCCAAACGATTAGCAAA+TGG 0.544098 5.2:-50378646 None:intergenic
TTACTAATTATCAAGCATTG+AGG 0.545752 5.2:+50378704 MS.gene73918:CDS
AACTCCAAGGTAATAACTGA+CGG 0.558076 5.2:-50378430 None:intergenic
TCGAAGACACATAACGGAAA+CGG 0.564967 5.2:+50375690 MS.gene73918:CDS
CGGTAACGAGAACCAGGAAG+AGG 0.571794 5.2:-50375969 None:intergenic
ATGAAATCGAAGACACATAA+CGG 0.573992 5.2:+50375684 MS.gene73918:CDS
TTCTCCTGTTGTGTATATGA+TGG 0.575030 5.2:+50378966 MS.gene73918:CDS
ACATTGTGAAATTCAAAATG+AGG 0.581885 5.2:-50377262 None:intergenic
GCTCGTGAAAAGGGGTGGAA+AGG 0.582006 5.2:+50379020 MS.gene73918:CDS
GAGTGCTCTCAAGGACCAGA+TGG 0.582704 5.2:+50382972 MS.gene73918:CDS
CTTAGAAATTGTATATGTGG+AGG 0.583613 5.2:-50378676 None:intergenic
TGACTCCGGTAACGAGAACC+AGG 0.583762 5.2:-50375975 None:intergenic
TTGATTGTCGAGGCCAATGG+TGG 0.583847 5.2:+50377069 MS.gene73918:CDS
TATTGACAAGACTCTCCTGT+AGG 0.584214 5.2:-50383101 None:intergenic
TGTAAAACGCCGGAATGTCG+CGG 0.587944 5.2:-50375792 None:intergenic
CACAGTGTGCTTGTATAGTG+AGG 0.594547 5.2:+50380214 MS.gene73918:CDS
TTAGAAATTGTATATGTGGA+GGG 0.595768 5.2:-50378675 None:intergenic
GTTTCCCAATCTTTATACCA+AGG 0.595788 5.2:+50379543 MS.gene73918:CDS
ATAGAAGATTTCTCTATGGT+GGG 0.596640 5.2:+50380285 MS.gene73918:CDS
TTCTCGTTACCGGAGTCACG+AGG 0.598162 5.2:+50375980 MS.gene73918:CDS
TCTGTAGATTTGTAATGCTG+TGG 0.604486 5.2:+50377204 MS.gene73918:intron
TATGGCCCATTAGAAAATGA+GGG 0.607756 5.2:-50380265 None:intergenic
TGTGCATCTTCGCTTTGAGG+AGG 0.608488 5.2:+50378813 MS.gene73918:CDS
ATCCTATCTTGCAAAAGGAA+GGG 0.608881 5.2:+50378457 MS.gene73918:CDS
GAATGTCGCGGTGGAGATCG+CGG 0.609199 5.2:-50375780 None:intergenic
AAGCAAATAAATACCTCTAG+GGG 0.612933 5.2:-50379113 None:intergenic
ACAATGTGTGGAAAGATCCA+AGG 0.613128 5.2:+50377278 MS.gene73918:CDS
ACTCCAAGGTAATAACTGAC+GGG 0.614716 5.2:-50378429 None:intergenic
TGTCCAATGAAGAACGTGAA+CGG 0.621619 5.2:-50375942 None:intergenic
GGACGAAGTCACTGTCGGCG+GGG 0.622339 5.2:+50375835 MS.gene73918:CDS
TCCGGTTTGTTGATGTGTGT+AGG 0.625329 5.2:+50375912 MS.gene73918:CDS
GTCCAATGCTGCCAATTGGG+AGG 0.626792 5.2:-50380189 None:intergenic
TGGATTCAGCTCGTGAAAAG+GGG 0.631678 5.2:+50379012 MS.gene73918:CDS
GGTTACGCGGCCGAGAATGG+CGG 0.633753 5.2:+50375711 MS.gene73918:CDS
CTCAGTGAAAGTGACCGCAA+AGG 0.636893 5.2:+50383003 MS.gene73918:CDS
AAACCGGAGAGATAAACCAC+CGG 0.637048 5.2:-50375897 None:intergenic
ACGGCGGACGAAGTCACTGT+CGG 0.644805 5.2:+50375830 MS.gene73918:CDS
TATCAGGAATGTCTACATTG+TGG 0.646595 5.2:-50383134 None:intergenic
CTTCACATAACCATCAAGTG+TGG 0.650369 5.2:-50378319 None:intergenic
ATTCAGCTCGTGAAAAGGGG+TGG 0.650960 5.2:+50379015 MS.gene73918:CDS
AGATAGGATGAACAACTCCA+AGG 0.653627 5.2:-50378443 None:intergenic
ATTTACGGAAACGGTTACGG+CGG 0.654253 5.2:+50375814 MS.gene73918:CDS
GCGGACGAAGTCACTGTCGG+CGG 0.657648 5.2:+50375833 MS.gene73918:CDS
TTGTGTATATGATGGAGGAA+AGG 0.660797 5.2:+50378974 MS.gene73918:CDS
TCCTGTTGTGTATATGATGG+AGG 0.660944 5.2:+50378969 MS.gene73918:CDS
AAATCAGTCAAATTCGAACA+AGG 0.661890 5.2:-50376554 None:intergenic
ATACCTCTAGGGGAGTTAGT+GGG 0.676100 5.2:-50379103 None:intergenic
TCTTAACCAGCAACGCTCTG+CGG 0.677605 5.2:+50377092 MS.gene73918:CDS
GAATGAGTGAGAAGAGCTCG+AGG 0.678527 5.2:+50378770 MS.gene73918:CDS
ATGCCCACTAACTCCCCTAG+AGG 0.680140 5.2:+50379100 MS.gene73918:CDS
ACATAACGGAAACGGTTACG+CGG 0.684145 5.2:+50375698 MS.gene73918:CDS
ACTTGATGGTTATGTGAAGG+TGG 0.685624 5.2:+50378323 MS.gene73918:CDS
TTTCCGTAAATGTAAAACGC+CGG 0.693090 5.2:-50375802 None:intergenic
GGTGGTTAAAGAACTTCCCG+AGG 0.694565 5.2:+50378341 MS.gene73918:CDS
CACACTTGATGGTTATGTGA+AGG 0.705447 5.2:+50378320 MS.gene73918:CDS
TGTCGCGGTGGAGATCGCGG+TGG 0.709675 5.2:-50375777 None:intergenic
TGTCGGCGGGGATAATGACA+CGG 0.710205 5.2:+50375847 MS.gene73918:CDS
AAAACGCCGGAATGTCGCGG+TGG 0.740237 5.2:-50375789 None:intergenic
CGGCGGGGATAATGACACGG+TGG 0.779770 5.2:+50375850 MS.gene73918:CDS
TAGGTTGGGATGATGCTACG+TGG 0.784598 5.2:+50379433 MS.gene73918:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 50375684 50383194 50375684 ID=MS.gene73918
chr5.2 mRNA 50375684 50383194 50375684 ID=MS.gene73918.t1;Parent=MS.gene73918
chr5.2 exon 50375684 50376055 50375684 ID=MS.gene73918.t1.exon1;Parent=MS.gene73918.t1
chr5.2 CDS 50375684 50376055 50375684 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50376509 50376593 50376509 ID=MS.gene73918.t1.exon2;Parent=MS.gene73918.t1
chr5.2 CDS 50376509 50376593 50376509 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50377046 50377113 50377046 ID=MS.gene73918.t1.exon3;Parent=MS.gene73918.t1
chr5.2 CDS 50377046 50377113 50377046 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50377211 50377299 50377211 ID=MS.gene73918.t1.exon4;Parent=MS.gene73918.t1
chr5.2 CDS 50377211 50377299 50377211 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50378281 50378478 50378281 ID=MS.gene73918.t1.exon5;Parent=MS.gene73918.t1
chr5.2 CDS 50378281 50378478 50378281 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50378630 50378834 50378630 ID=MS.gene73918.t1.exon6;Parent=MS.gene73918.t1
chr5.2 CDS 50378630 50378834 50378630 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50378954 50379121 50378954 ID=MS.gene73918.t1.exon7;Parent=MS.gene73918.t1
chr5.2 CDS 50378954 50379121 50378954 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50379436 50379603 50379436 ID=MS.gene73918.t1.exon8;Parent=MS.gene73918.t1
chr5.2 CDS 50379436 50379603 50379436 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50380182 50380372 50380182 ID=MS.gene73918.t1.exon9;Parent=MS.gene73918.t1
chr5.2 CDS 50380182 50380372 50380182 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
chr5.2 exon 50382969 50383194 50382969 ID=MS.gene73918.t1.exon10;Parent=MS.gene73918.t1
chr5.2 CDS 50382969 50383194 50382969 ID=cds.MS.gene73918.t1;Parent=MS.gene73918.t1
Gene Sequence

>MS.gene73918

ATGAAATCGAAGACACATAACGGAAACGGTTACGCGGCCGAGAATGGCGGTGGCGGAGGCGGAGGTGGAGGTGGAAGTAGTAGCTCACCGAGTCCACCGCGATCTCCACCGCGACATTCCGGCGTTTTACATTTACGGAAACGGTTACGGCGGACGAAGTCACTGTCGGCGGGGATAATGACACGGTGGAGTCTCCGCTACTTTTTCTTACTGCCGGTGGTTTATCTCTCCGGTTTGTTGATGTGTGTAGGACCGTTTCCGTTCACGTTCTTCATTGGACATACTCCTCTTCCTGGTTCTCGTTACCGGAGTCACGAGGTTTTCGATCATCTTTGGCCTGAAATTGATTCTGATAATTCCTCTTTCATTGAGGTTAGTGAAAATAAGTGTAATGTGCGATAATCGCGTTTGGTGAATAATTTTGTTTATGGATTTGGTGCTATATGAAGTTTTATGATGGAGCTGAATTCGTGTCACTGAATCTTGTTTGGTGAATAATTTTGGATTATTATGGAGCTGAATTCCTGTTACTGAATCTTGTTTGGTGAATAATTTTGGCGTTACTAAATCTTGTTTGGTGAATAATTTTGGATTATTATGTTGCTGAATTCGTGTTACTGAATCTTGTTTGTTGAATAATTTTGGCGTTACTAAATCTTGTTTGGTGAATAATTTTGTTTTTGGTGTTATTTGAAATTTGGTTGTTTTGTTGATTTTGTTGTTTATTATTAACTACTTATTATAGATGCGTGATTAATAATGAAATTATTTTTGTCGATAAATAAACAGGGTTGATTTTTAATTGTTTATTTATTTATTTTGCAGTTGTCTTCAGTGTGGAAATACAAAAGGAAATTGAGAGAGAAGAAGCCTTGTTCGAATTTGACTGATTTGCATCATGAACATTTTGGTATATAATACTAAAGCTCTATGATCTATTCTCTCTTAGCTTTCGTTTCACATGTCTACCCGTTTGACTCAGACATTGGATATGATATTGACACTGACACTGATAATAATTTGAGAAAACGATATTATTCAATGTAATCATATGTGTTGGTATTGTGTTGTGTCAGACTTGCCTTCGATCAAAGATGTCGGTGCTACATAGTTTAATATTGCTGCTAATGTGTGATTTTGCTTTGTAATATAGTCAACTTTATTTAAAAGAGCTTATCAAAATAGCTGATGAATCATAAGTTGTTTTAAGCTGATTTTCATAAGCTCTGTTGTAGAAATAGTTTATATAGAATTCATGCAGAATTTGCTCCATTTCCACTTTGATCGTAACCTTATATGATAAGCTGCTTTTTAGTTATTTATGGAAAATGCTCTCTAATATTATGTTTTCTATAATTTCAGTGTCCCCTGGCCTGAATGGTTTTTTGATTGTCGAGGCCAATGGTGGTCTTAACCAGCAACGCTCTGCGGTATGACATAGTTTTTCCTTTGTCTGTCTTTTGGCAATTTTTTTATTCGACGGATGAACTATTTTTCACCGTTGTGATTTTAATTGTGGCTCTGTAGATTTGTAATGCTGTGGCTGTTGCTGGACTCCTGAATGCAATTCTAGTTATACCTCATTTTGAATTTCACAATGTGTGGAAAGATCCAAGGTGACCAATTTACCTATTTATTATCTTTGGCTTGAGAAGTTTTTTCAACACAAACATTGCTAGTTAAGTTTCATTGTTTGGCCTGTTGTTGTCAAAATATTACCACATGTGGTTGGGGAGAAAACGAGTTTATCTGTTGCTTAATACAGTAGGTGTGTGTTAGCTTTATACTCTTGTTTATGTAAACTAATATTGTGCTTTGATGCAATTGTTTATGAACTGAAATTGTCTTCCATCAAATGGCTATTTACAATATTTTTAAATCTAAAACAAGTTACTCACTGCTGCCTTTGTTAGGCTGATTTTTCTATCTCTCGAATTGTATAATGACGACTCTTCAGGAAAAAACTGATAGCGAATGGAGAAAGATTTTCAATTTATGAGAGGAATTTTTTTTGCTAGGTTGTTATCATTCAATTGGAGGGATAAGGAATGAAATGGGAATAATGGATTTGAATCCTTTCCTAAGAGAACTCATATCACATGCCAAAAAGAAAAGAAGAAAATAGAATTTCATTCAATGGTTTTTAAGAAACTTTCCTACCATGTAACTCATAAAATATTTGATGTAAATATGTTTCAATAGGTTGTTTATTTAATCATTTTTTCCATTAACAGTTCCATGTCAACATTGCTTTTTATGTTATAAGTAGTGCATTAATTTTACTTCCTATACAAAACCTATGCTAGAGGCTGATTCTCCTACGATATACTTTTTAAATTACATTAGTTAGCACTTCTAACTTTATAGTATGTTTATGATGCGGTTGATCAAGCATTACTTGTTTAACGTTTGTCTTCAACTAGTAGATACTGAAATCATACCTCATTTGGTGTCTGTATTTTCTCGTAGAACCATCCATACAGATAATTAATTATTAAATATACCCGTAGTCAACAATACGAGGCTAATTAAACTTTTATGCCTGATTTCATTTCTGCATTCACCATCCATTTTAAATAATGTTATTTTGTGTACAGTGAATTTGGGGATATATATGATGAAGATCATTTTATATCCACACTTGATGGTTATGTGAAGGTGGTTAAAGAACTTCCCGAGGCAATAATGGAGAGACATAATTACAATATGACTAATATAACTAGCATCAGAGTTGAAGCTTGGGCTCCCGTCAGTTATTACCTTGGAGTTGTTCATCCTATCTTGCAAAAGGAAGGGTAGGTATCAGACGACTTTGCCCTCATTTCTTATTCTCTATATTAGTTTTAGGGTGAACATGTTCTGTGAATCCATATTAGGCAGTCGACACAACTCAATTTTAAGCAAACAATTTGATGAAAGGAACTTTATTTTGGCAAACTTATGCAGGGTTATTCGAATAGCTCCATTTGCTAATCGTTTGGCAATGAGTGTCCCTCCACATATACAATTTCTAAGATGTCTTACTAATTATCAAGCATTGAGGTTTTCTTCTTCAATATCTGCACTAGCTGAGAATCTGGTTTATCGAATGAGTGAGAAGAGCTCGAGGACAGATGGAAAATATATTGCTGTGCATCTTCGCTTTGAGGAGGTACTGATGCTCATTCTATCATATTGAAAATTAGGTTTTGCTATATGCATCTCTAGGTGTCAAAAGTATACAGAATTGAAGTATTATGTTAATAATTGCCTAACTATCCTAACATACAGGACATGATAGCATTCTCCTGTTGTGTATATGATGGAGGAAAGGCAGAGAAGTTGGAAATGGATTCAGCTCGTGAAAAGGGGTGGAAAGGAAAGTTCAAAAGAAAAGGTCGTATCGTTGTACCTGACCTCTATCGAGTCAATGGAAAATGCCCACTAACTCCCCTAGAGGTATTTATTTGCTTGCTGATTCTTCTCATATCTTGAATTATTTTTTCCCTTAATTTAAAATCTCGCCAGTCTCATGTCCTTAAGTTATTGATTTCTTAATGAGATATTGCTTTTCTTTTTTCCCTTAATTTATTCTTGATTCCAATTGATTATGGCCTCATCTTTATGCACTGTTTTAAATTAATTTTTGTAGAAGAGCAACATATTGAGATGAAAATCAAACAAATCTATGGAAGTTTAAATTGTGATAAAACTGAGATTATATTATGGAAGAGACTAATGTTTATTTCCTGTTGATTTCCATAATTATGTAGGTTGGGATGATGCTACGTGGCATGGGATTCAATAACAATACATCAATATATTTAGCTTCTGGGAAAATATATCATGCAGAAAAGTATTTGGCTCCTTTGATAAAGATGTTTCCCAATCTTTATACCAAGGAGTCTCTTGCAACACCTGATGAGCTTGCTCCTTCTATGGTAAAAATCTTTACTGGTGAAAAAATATATATTTCATTCTTCCATTCCATGACCTTGCTGTGATAAACTTTATAACTAATGAATTGGACTTAGTGGCATGGAGTTGTAACTTGTAACAACCCTTCATTAAAACTCTTAAATTTGGCTTGGAGTTGGACCTAGGAACCGAAGTTCATCCATTTTAAAAGAGGATCACCAGTTTGAACTTCGATTGGTTCATTTTTTTTTTGGAATTATAGCTGATGCTCCAACACCAAGATTGCATTACTGGAAGGAAACCTGAATATTGTAATATTTATAATAATCAAGGATGTAATTAACTTTCAATTCCAAAATATAAATGATGTATTTGGCTTTTTATTGTGGTTCTCTCTTCATCCCTCATTCTAATACTTAGTATATTTGGCATGATGTTTAGTTGAAAACATCTTTACTGCCCTCTTGTGTGACTGTGATGTAAAATGTAAAAAAAAATAAAAAATCACGGGCAGGTTTAGTTATTCTTTCTTTAAGTGTATGCCTTAATATTTTGTGTGATTTTCGAATATGCTCTTATGCTTAGTTTCATCCTTCTATAGGGTTATTCCTCCCAATTGGCAGCATTGGACTACACAGTGTGCTTGTATAGTGAGGTTTTTGTTACAACCCAAGGGGGTAACTTCCCTCATTTTCTAATGGGCCATAGAAGATTTCTCTATGGTGGGCATGCCAAAACCATTATTCCTGATAAACGCAAACTTGTTGTTCTTTTCGAGAACACGACTATCAGGTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATATATATATATATATATAAGATTTGTGGAATACCATACATCATTTTCGTTGTCTTTTTAGAAATATAATAAAACTTTAGGTGATATGTATGTAGGATTCATATTATAATTTCCAGCCTGTTTTATGACATGAGTGCTAACTGATTGAAATATCCCCTAGACATGAATGAGAACCTTTTGCTATGCACTAGGTAAAATTGAGAAACAGATAATTTTTTTCTATTATACCTTCTTTTAACTAAAATAGGATACAGCCAAACTCACGAGATCATTTACAAGTACTAGTTATACTCTTTATTGATTATCTACTTATCTTGAAAAATCAAAATTGTTTTGAGAAAGTTGTGACCAATTAAGTAAAAGTGAAGGAGCAAAGATCTAAATCATTATTAATGGTAATCCTCTTGACGGTCCTACTTTTGGTTCCCTCTCTACTTTAATTTGCAAGACTAATCGATCTAAGAAGACCAAATTTAAGCATAGCTTTTGTATGTCTCACTCAAACAAGTTTTCGTATAGACTTGAAGCATGGCTTGCAGAAAAGGTTACTAAAATGAAAGGATCATGTACCATGAGTTGCTAACTTGGTGGTGCTCACCATAATTAACGATTAGAAACCAAAAGCTAGACTAGGCATATACCTTTGTAGACATAGCCCCAGTTGGTTACTTGTAGTTTTGCAATTGTTTGTAACATGGTTTTTGTCAAACAATAACTAAAATAATATTAAGCTCAACAAGTAACACAAATTTAAACAACCAAGAGAACACATACCAAATATTAGGATTCAATCATGATTCATAAATTAGGATTCATTGTTGTCCTATCCAAAGGGCTTAACAATGCTTAATAATATCCCTAAAAACAATAGCTATAATGTCAATATCCTCAAACATCAAAATAAAATATAAAAGAAGTGGGTTCATGAACAAAGCCTATTGGAACCCGAGAAATAGTTCCCTTGCAATCACCTTGAAAGACTTAGAAATCTATCAAAAAATATCAAAGGTTGCTTGCCGAAATCATTATGAAATTGCAGTTGAAGTCGTCACTTGTCATCATCATGTCATTTATGAGTTGTTACTTCTCTGCGTTGTTAACATGTTATCAGCACACTAGCTTGGTTGTGTCATTAGGTTTTTTGGGTTTTCAAGAACTATAGTATTATAATTTATAAGGAGAAATAGCCTTTGAAATTGAGCTTCCACATAGTTGAATGTCCGTGCAGCATAAGGGGAATGCCTTGGCACATACCACCATACGCATAAATCTGCATGTCTATGCTGAATGTGTCACCCCCGTGCAGGTCTCCAAAGCAACTACTTTGCATTCCAAAGTGTAGATTTCTTCCTATCTTCATTTTAGCCCAAAATCTCCAATTTTCGTCTTGTTTTAACCATGTTTAAGTAATTCAATTCCACTCCTCCAAATGCCAATCTTCAGTGGTAGCTGAATTTCAAATCCAACTAACACCTTGGAACCCTTAACTCGTCCAGTATTTCCTCTATAACTAGAAAGGGGGATTTGTTGGGTATTGTAACTTTGTTTAGAGGCTCTGTAATTCATACAAGATTCTCAGCCGCCACTTTTTTCTGCACTAATATCATGGGACTCGAATAATAACTTACACTATGTCGAGTGATGCCTGCAGCTAGATGTAAACAATTCGAACTGAGCACCTAAGACTTGATTTGTTTGTGAAAAAAAGAGTTGCTCAAGTTTGACTCATTTTAGTCATTTATTTAAACGAGTCCAACTAGAAACTTGAGTTTGACTTATTAAAAATTTGGCCGTGACTTTTTTTTTAAAAATAAATAAGCAAAGTGGGATTGACTTGGTTTTCTATTTTATAACTTACTTTTTTGCATTTAGAATAAATTTTCTTAAATTACTTTTGAAGCTAATCAATTTAGTATTTAATAAATATTTAATATTATAAGCAAATCTACAAATAGAAATATGTGCTTAAGTAAACCCAATCTTAATTTGTATGCCCCATTCTTATATACAAGACTTTAATATTTATACATGTATATTTGGAGGGATCAAATTACACCAGTGTCATATTTGGATATTGTTGCACTGTTTAATAGTTTTTTTTCCAGATACTTGTACTACAAAACTCACTGTTGGATAAGTTATGATCGTTTATATTATTTATGTAATTTCTTACGTAAATGTATAATCATTTGATATGTCAATGAGATATTTACAGGTTAACATTGTTTGATAAAAGTCCATAAGTCGTTAATTTTGATGTATCCTCAGCATCTCTCCTATCAGAACCGCACAGAACAAGCTGTTCTGATCTGTGTCCACTGTCCAAGTCCCAAGTGCTTTCTCTAATTGTTTGTTTATGTCTGTGAATTAAAATTGCCAATGAGTTTTATACTGGATTTGTTTCCCATCCTTGTATGTGTCTTATATTTAAGCATAGTTGCCATGTGGATGGATTCAATATGAAAGCTTAGGCTATGCAATCTTTGAAACACACAAGATGACACAAAATATTTGTGTATCAATTTTTGCAGTTGGAGTGCTCTCAAGGACCAGATGGATGACATGCTCAGTGAAAGTGACCGCAAAGGAATCATGGTCCCTAGAGTTAGAAAAATTAACAGAAAAACTTCTGTCTACACTTACCCTTTACCAGAATGTAGATGCCTACAGGAGAGTCTTGTCAATAAAACAATTGACCACAATGTAGACATTCCTGATAACTACAGTCTACAGCAGAACAAAAGCTTAAACAGATGA
Protein sequence

>MS.gene73918.t1

MKSKTHNGNGYAAENGGGGGGGGGGSSSSPSPPRSPPRHSGVLHLRKRLRRTKSLSAGIMTRWSLRYFFLLPVVYLSGLLMCVGPFPFTFFIGHTPLPGSRYRSHEVFDHLWPEIDSDNSSFIELSSVWKYKRKLREKKPCSNLTDLHHEHFVSPGLNGFLIVEANGGLNQQRSAICNAVAVAGLLNAILVIPHFEFHNVWKDPSEFGDIYDEDHFISTLDGYVKVVKELPEAIMERHNYNMTNITSIRVEAWAPVSYYLGVVHPILQKEGVIRIAPFANRLAMSVPPHIQFLRCLTNYQALRFSSSISALAENLVYRMSEKSSRTDGKYIAVHLRFEEDMIAFSCCVYDGGKAEKLEMDSAREKGWKGKFKRKGRIVVPDLYRVNGKCPLTPLEVGMMLRGMGFNNNTSIYLASGKIYHAEKYLAPLIKMFPNLYTKESLATPDELAPSMGYSSQLAALDYTVCLYSEVFVTTQGGNFPHFLMGHRRFLYGGHAKTIIPDKRKLVVLFENTTISWSALKDQMDDMLSESDRKGIMVPRVRKINRKTSVYTYPLPECRCLQESLVNKTIDHNVDIPDNYSLQQNKSLNR