Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74085.t1 | XP_003621904.1 | 92.9 | 562 | 22 | 1 | 1 | 544 | 1 | 562 | 6.10E-290 | 1006.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74085.t1 | Q9MAU3 | 60.9 | 550 | 208 | 3 | 1 | 544 | 1 | 549 | 1.4e-181 | 637.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74085.t1 | G7KXV7 | 92.9 | 562 | 22 | 1 | 1 | 544 | 1 | 562 | 4.4e-290 | 1006.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene74085 | MS.gene061229 | PPI |
| MS.gene030125 | MS.gene74085 | PPI |
| MS.gene74085 | MS.gene68598 | PPI |
| MS.gene74085 | MS.gene26816 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74085.t1 | MTR_7g024860 | 94.662 | 562 | 12 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1085 |
| MS.gene74085.t1 | MTR_7g024867 | 94.662 | 562 | 12 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1085 |
| MS.gene74085.t1 | MTR_3g055240 | 57.721 | 544 | 196 | 5 | 1 | 544 | 1 | 510 | 0.0 | 596 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74085.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene74085.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene74085.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene74085.t1 | AT1G54360 | 47.059 | 561 | 243 | 7 | 3 | 544 | 1 | 526 | 1.06e-160 | 468 |
| MS.gene74085.t1 | AT1G54360 | 47.059 | 561 | 243 | 7 | 3 | 544 | 15 | 540 | 1.43e-160 | 468 |
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGTTCATCTTTCCAAATA+TGG | 0.136550 | 7.4:+76584478 | None:intergenic |
| CATGAAGCTTGGCGTGTTTA+TGG | 0.154260 | 7.4:-76585676 | MS.gene74085:CDS |
| CTTCTGTCCTAGCCCATATT+TGG | 0.203384 | 7.4:-76584491 | MS.gene74085:CDS |
| TTCTTATGTCAGCCAATATA+TGG | 0.220354 | 7.4:-76588963 | MS.gene74085:intron |
| AACGTTGCTGAATGCATTCT+TGG | 0.247007 | 7.4:-76586586 | MS.gene74085:CDS |
| GTAAGTTTGGCTACTGATTC+AGG | 0.252357 | 7.4:-76588020 | MS.gene74085:CDS |
| TGTTGTGTCATTGCCTTCTT+TGG | 0.257279 | 7.4:+76586561 | None:intergenic |
| CGTATATATGCTTCAAGATT+TGG | 0.258387 | 7.4:+76585751 | None:intergenic |
| TAAAGAAGCATTAGTAAGTT+TGG | 0.260790 | 7.4:-76588033 | MS.gene74085:CDS |
| TAACCTGCCTTGTTGCTAAA+AGG | 0.298000 | 7.4:-76587374 | MS.gene74085:CDS |
| ATAACTTCGATTGTTTCTTT+AGG | 0.299682 | 7.4:+76591555 | None:intergenic |
| CTAGATAAGACATGCTTAAC+AGG | 0.311507 | 7.4:+76588203 | None:intergenic |
| GCTACAAAGCATGTGAAATA+AGG | 0.321309 | 7.4:+76587981 | None:intergenic |
| TGGAAAGATGAACTGAATTC+AGG | 0.326248 | 7.4:-76584471 | MS.gene74085:CDS |
| AACTTACATAAGGTTCAATA+TGG | 0.335073 | 7.4:+76587776 | None:intergenic |
| ACAACACTATGGTGCAATTC+AGG | 0.341769 | 7.4:-76586544 | MS.gene74085:CDS |
| CAGTTCATCTTTCCAAATAT+GGG | 0.342840 | 7.4:+76584479 | None:intergenic |
| CAAGGCAAGCCCTATGGAAT+TGG | 0.343736 | 7.4:-76584733 | MS.gene74085:CDS |
| TGTCTTATCTAGAGAGCTTC+AGG | 0.353561 | 7.4:-76588192 | MS.gene74085:intron |
| TCTTTAAAGAGAACCGAATC+AGG | 0.355917 | 7.4:+76588050 | None:intergenic |
| ACAGGTAATTTGATATCAAC+TGG | 0.361767 | 7.4:+76588221 | None:intergenic |
| CCAGATGCAAATCCATATAT+TGG | 0.371634 | 7.4:+76588951 | None:intergenic |
| GCGTGTTTATGGAGCCTTGC+TGG | 0.371996 | 7.4:-76585665 | MS.gene74085:intron |
| GGGAGAGGAGATGGAAAAGC+GGG | 0.385964 | 7.4:+76585499 | None:intergenic |
| CCAGAAAATGCTCCTGTAGA+TGG | 0.393196 | 7.4:-76588675 | MS.gene74085:intron |
| ACATCATTGGTTGAGCTGTT+TGG | 0.393677 | 7.4:-76584438 | MS.gene74085:CDS |
| TGGAGATGTTAGTGTAGTTC+AGG | 0.394478 | 7.4:-76584676 | MS.gene74085:CDS |
| AAGTTATTGCTCAGAGCATT+GGG | 0.407577 | 7.4:-76591539 | MS.gene74085:CDS |
| TGTGTCTCAAGAAGCATCTC+TGG | 0.409556 | 7.4:+76585718 | None:intergenic |
| TGCCTTGTTGCTAAAAGGTT+GGG | 0.413976 | 7.4:-76587369 | MS.gene74085:CDS |
| GGCTGCTGCTCCAATTCCAT+AGG | 0.419772 | 7.4:+76584723 | None:intergenic |
| CTCCTCTCCCTCATACTGTC+TGG | 0.432250 | 7.4:-76585486 | MS.gene74085:CDS |
| ACAAGGCAGGTTACAATTGA+TGG | 0.435760 | 7.4:+76587384 | None:intergenic |
| AGCTTTGAATGAGCCTGATT+CGG | 0.436229 | 7.4:-76588063 | MS.gene74085:CDS |
| GCAGTATCAAGGGGAGCTTT+TGG | 0.439198 | 7.4:+76588744 | None:intergenic |
| CAGTATCTACTTTCTTCAAA+GGG | 0.439281 | 7.4:+76584700 | None:intergenic |
| TGTTTCATTCAGCGTGCTGC+AGG | 0.440328 | 7.4:-76585553 | MS.gene74085:intron |
| GAGAACTAACCTGCATGATC+TGG | 0.441191 | 7.4:+76591454 | None:intergenic |
| CCAATATATGGATTTGCATC+TGG | 0.443044 | 7.4:-76588951 | MS.gene74085:CDS |
| GAAGTTATTGCTCAGAGCAT+TGG | 0.452382 | 7.4:-76591540 | MS.gene74085:CDS |
| CGCATACGGTATTCAACATC+AGG | 0.457789 | 7.4:+76591477 | None:intergenic |
| CTGCCTTGTTGCTAAAAGGT+TGG | 0.459360 | 7.4:-76587370 | MS.gene74085:CDS |
| ACAGTATGAGGGAGAGGAGA+TGG | 0.459983 | 7.4:+76585490 | None:intergenic |
| TGTCACTGGCTTGCCATTGA+AGG | 0.461110 | 7.4:-76588714 | MS.gene74085:CDS |
| GCAACAGAAAGCGCAACATC+AGG | 0.464025 | 7.4:+76591501 | None:intergenic |
| TATGGTGCAATTCAGGGGTT+AGG | 0.464972 | 7.4:-76586537 | MS.gene74085:CDS |
| CCTGCATGATCTGGCGCATA+CGG | 0.466093 | 7.4:+76591463 | None:intergenic |
| CATCTTTCCAAATATGGGCT+AGG | 0.469577 | 7.4:+76584484 | None:intergenic |
| ATTGGTGCAGCGTCATCCTC+TGG | 0.470899 | 7.4:-76584588 | MS.gene74085:CDS |
| CTGGTCTTCCAGACAGTATG+AGG | 0.477347 | 7.4:+76585478 | None:intergenic |
| CAAGATTTGGCAGCAAAAGA+AGG | 0.477595 | 7.4:+76585764 | None:intergenic |
| AGGACGTGTCCTGACATCAT+TGG | 0.478074 | 7.4:-76584451 | MS.gene74085:CDS |
| GACAGGTAAGTGCAGTATCA+AGG | 0.484319 | 7.4:+76588733 | None:intergenic |
| TCAGTATCTACTTTCTTCAA+AGG | 0.486930 | 7.4:+76584699 | None:intergenic |
| ATACTGCACTTACCTGTCAC+TGG | 0.488327 | 7.4:-76588728 | MS.gene74085:CDS |
| TTCCAGACAGTATGAGGGAG+AGG | 0.490867 | 7.4:+76585484 | None:intergenic |
| ACAGGTAAGTGCAGTATCAA+GGG | 0.492623 | 7.4:+76588734 | None:intergenic |
| GTCTCCTCTTCCTTATGAGA+TGG | 0.492865 | 7.4:+76584642 | None:intergenic |
| AAATTCTTCACCATCTCATA+AGG | 0.493204 | 7.4:-76584652 | MS.gene74085:CDS |
| TTACAATTGATGGCATCAAC+TGG | 0.493457 | 7.4:+76587394 | None:intergenic |
| TTCAATGGCAAGCCAGTGAC+AGG | 0.496154 | 7.4:+76588716 | None:intergenic |
| TAAACGCAAGGCAAGCCCTA+TGG | 0.498294 | 7.4:-76584739 | MS.gene74085:CDS |
| AGCAGGTTGCACTCCTTCAA+TGG | 0.501977 | 7.4:+76588701 | None:intergenic |
| AAGGCAATGACACAACACTA+TGG | 0.502589 | 7.4:-76586555 | MS.gene74085:CDS |
| TTGGGAACTTAGAGACTTCA+CGG | 0.503314 | 7.4:-76587325 | MS.gene74085:CDS |
| CAATCCTTTCAGGTTTCTCG+TGG | 0.508236 | 7.4:-76587868 | MS.gene74085:intron |
| AGGGAGAGGAGATGGAAAAG+CGG | 0.510840 | 7.4:+76585498 | None:intergenic |
| CAACACTATGGTGCAATTCA+GGG | 0.511881 | 7.4:-76586543 | MS.gene74085:CDS |
| GGTGAAGTTAGAAGCAGTGA+GGG | 0.512556 | 7.4:-76584537 | MS.gene74085:CDS |
| TGCATTCTTGGATCCAAAGA+AGG | 0.513153 | 7.4:-76586574 | MS.gene74085:CDS |
| GAGAGGGACAATACCACATT+GGG | 0.513459 | 7.4:+76586504 | None:intergenic |
| GTTAGATAGCTACCATCTAC+AGG | 0.515613 | 7.4:+76588663 | None:intergenic |
| CACATGCTTTGTAGCTGATG+AGG | 0.521153 | 7.4:-76587973 | MS.gene74085:intron |
| GCTGCTGCTCCAATTCCATA+GGG | 0.522208 | 7.4:+76584724 | None:intergenic |
| GCTATTAACAACTCGCATCA+AGG | 0.536109 | 7.4:+76587819 | None:intergenic |
| TGATTCAGGACTTCATCCAT+TGG | 0.539715 | 7.4:-76588006 | MS.gene74085:CDS |
| TGGTTGCATCAATATGCAAA+AGG | 0.541657 | 7.4:-76587296 | MS.gene74085:intron |
| TAGAGACTTCACGGCTAACC+TGG | 0.544251 | 7.4:-76587316 | MS.gene74085:CDS |
| CATGTGAAATAAGGAACCAA+TGG | 0.544256 | 7.4:+76587990 | None:intergenic |
| CGCATCAAGGCAAATAGAAG+TGG | 0.550878 | 7.4:+76587832 | None:intergenic |
| TTTATAGCAATCTACAGTCC+CGG | 0.553507 | 7.4:-76586617 | MS.gene74085:CDS |
| AAATGAAAAGACATGAAGCT+TGG | 0.554764 | 7.4:-76585687 | MS.gene74085:CDS |
| TTCACCATCTCATAAGGAAG+AGG | 0.558979 | 7.4:-76584646 | MS.gene74085:CDS |
| ACAGCTCAACCAATGATGTC+AGG | 0.559937 | 7.4:+76584442 | None:intergenic |
| TTGAAGAAAGTAGATACTGA+TGG | 0.560684 | 7.4:-76584696 | MS.gene74085:CDS |
| TAGATTGACAGACAACCATT+GGG | 0.562642 | 7.4:-76587343 | MS.gene74085:CDS |
| GTAGATTGACAGACAACCAT+TGG | 0.564706 | 7.4:-76587344 | MS.gene74085:CDS |
| AAGCAGTGAGGGTGATACTC+AGG | 0.570545 | 7.4:-76584526 | MS.gene74085:CDS |
| CCGTATGCGCCAGATCATGC+AGG | 0.590642 | 7.4:-76591463 | MS.gene74085:intron |
| ATTCAGGGGTTAGGAGCACT+AGG | 0.597296 | 7.4:-76586528 | MS.gene74085:CDS |
| AGAAGTAAGAACACTTGCAC+TGG | 0.600577 | 7.4:+76585459 | None:intergenic |
| GGAGAGGGACAATACCACAT+TGG | 0.615478 | 7.4:+76586503 | None:intergenic |
| TGATCATGAAGATAAACGCA+AGG | 0.624991 | 7.4:-76584751 | MS.gene74085:intron |
| AACACTATGGTGCAATTCAG+GGG | 0.625157 | 7.4:-76586542 | MS.gene74085:CDS |
| CTACGATTCAAACGAGCTGT+CGG | 0.629625 | 7.4:-76588921 | MS.gene74085:CDS |
| AGTATCTACTTTCTTCAAAG+GGG | 0.630790 | 7.4:+76584701 | None:intergenic |
| GTATCTACTTTCTTCAAAGG+GGG | 0.631481 | 7.4:+76584702 | None:intergenic |
| ATATATGGATTTGCATCTGG+TGG | 0.632207 | 7.4:-76588948 | MS.gene74085:CDS |
| GATAACATATCTTACCAGCA+AGG | 0.646319 | 7.4:+76585651 | None:intergenic |
| TGAAGTCTCTAAGTTCCCAA+TGG | 0.651064 | 7.4:+76587328 | None:intergenic |
| CAGGTAAGTGCAGTATCAAG+GGG | 0.654159 | 7.4:+76588735 | None:intergenic |
| TGGTCTTCCAGACAGTATGA+GGG | 0.668975 | 7.4:+76585479 | None:intergenic |
| ACAGCTCGTTTGAATCGTAG+AGG | 0.677708 | 7.4:+76588924 | None:intergenic |
| TGGTGAAGTTAGAAGCAGTG+AGG | 0.680296 | 7.4:-76584538 | MS.gene74085:CDS |
| AGGAGCACTAGGACCCAATG+TGG | 0.719832 | 7.4:-76586517 | MS.gene74085:intron |
| GGAGAGGAGATGGAAAAGCG+GGG | 0.719916 | 7.4:+76585500 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 76584386 | 76591589 | 76584386 | ID=MS.gene74085 |
| chr7.4 | mRNA | 76584386 | 76591589 | 76584386 | ID=MS.gene74085.t1;Parent=MS.gene74085 |
| chr7.4 | exon | 76591464 | 76591589 | 76591464 | ID=MS.gene74085.t1.exon1;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76591464 | 76591589 | 76591464 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76589941 | 76590027 | 76589941 | ID=MS.gene74085.t1.exon2;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76589941 | 76590027 | 76589941 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76588872 | 76588973 | 76588872 | ID=MS.gene74085.t1.exon3;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76588872 | 76588973 | 76588872 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76588676 | 76588784 | 76588676 | ID=MS.gene74085.t1.exon4;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76588676 | 76588784 | 76588676 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76588193 | 76588296 | 76588193 | ID=MS.gene74085.t1.exon5;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76588193 | 76588296 | 76588193 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76587974 | 76588111 | 76587974 | ID=MS.gene74085.t1.exon6;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76587974 | 76588111 | 76587974 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76587783 | 76587878 | 76587783 | ID=MS.gene74085.t1.exon7;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76587783 | 76587878 | 76587783 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76587297 | 76587421 | 76587297 | ID=MS.gene74085.t1.exon8;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76587297 | 76587421 | 76587297 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76586518 | 76586650 | 76586518 | ID=MS.gene74085.t1.exon9;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76586518 | 76586650 | 76586518 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76585666 | 76585791 | 76585666 | ID=MS.gene74085.t1.exon10;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76585666 | 76585791 | 76585666 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76585452 | 76585563 | 76585452 | ID=MS.gene74085.t1.exon11;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76585452 | 76585563 | 76585452 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| chr7.4 | exon | 76584386 | 76584762 | 76584386 | ID=MS.gene74085.t1.exon12;Parent=MS.gene74085.t1 |
| chr7.4 | CDS | 76584386 | 76584762 | 76584386 | ID=cds.MS.gene74085.t1;Parent=MS.gene74085.t1 |
| Gene Sequence |
| Protein sequence |
