Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74441.t1 | GAU48083.1 | 77.9 | 149 | 29 | 2 | 10 | 158 | 124 | 268 | 1.00E-51 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74441.t1 | A0A2Z6P0T0 | 77.9 | 149 | 29 | 2 | 10 | 158 | 124 | 268 | 7.2e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050787 | MS.gene74441 | 0.817828 | 2.69E-52 | -1.69E-46 |
| MS.gene053054 | MS.gene74441 | -0.811872 | 5.63E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene74441 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene055356 | MS.gene74441 | 0.80525 | 1.46E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene74441 | 0.80446 | 2.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATAATTTGAATATTTAT+TGG | 0.149686 | 6.4:+207678 | None:intergenic |
| AAATATAGATTTGGATTTAA+TGG | 0.180402 | 6.4:-207527 | MS.gene74441:CDS |
| AATGCAAAGGGTAGAGTATA+TGG | 0.262241 | 6.4:-207748 | MS.gene74441:CDS |
| GAGAGGTTGAGGCGCGTTTA+AGG | 0.273052 | 6.4:-207633 | MS.gene74441:CDS |
| ATTTGCATGTTTAGCAAATA+CGG | 0.348024 | 6.4:+207546 | None:intergenic |
| TAAACATGCAAATATAGATT+TGG | 0.354681 | 6.4:-207536 | MS.gene74441:CDS |
| ACAAACAATCGATTCCATTA+AGG | 0.358541 | 6.4:-207590 | MS.gene74441:CDS |
| TAGAGTATATGGGCTTGGTT+TGG | 0.416967 | 6.4:-207737 | MS.gene74441:CDS |
| ATTAGTGTATCATAATCAAT+AGG | 0.448901 | 6.4:+207793 | None:intergenic |
| TGAGTCTCAACCTCATCCTT+TGG | 0.456749 | 6.4:-207851 | MS.gene74441:CDS |
| GTCTCAACCTCATCCTTTGG+AGG | 0.479026 | 6.4:-207848 | MS.gene74441:CDS |
| GGATTTAGACGTTGAGCCAC+GGG | 0.479351 | 6.4:+207699 | None:intergenic |
| CAACCTCATCCTTTGGAGGC+TGG | 0.487170 | 6.4:-207844 | MS.gene74441:CDS |
| AAGGGTAGAGTATATGGGCT+TGG | 0.493623 | 6.4:-207742 | MS.gene74441:CDS |
| TGTATCATAATCAATAGGCT+TGG | 0.504722 | 6.4:+207798 | None:intergenic |
| GTATCATAATCAATAGGCTT+GGG | 0.509867 | 6.4:+207799 | None:intergenic |
| TGGTATGAGTGGAGAGGTTG+AGG | 0.514731 | 6.4:-207644 | MS.gene74441:CDS |
| GTATATGGGCTTGGTTTGGA+TGG | 0.514998 | 6.4:-207733 | MS.gene74441:CDS |
| ATGCAAAGGGTAGAGTATAT+GGG | 0.526831 | 6.4:-207747 | MS.gene74441:CDS |
| ACACTAATGATTGAAGCTGT+TGG | 0.537396 | 6.4:-207778 | MS.gene74441:CDS |
| GATGGCAGCATGCGCACCCG+TGG | 0.550034 | 6.4:-207715 | MS.gene74441:CDS |
| CTAATGATTGAAGCTGTTGG+AGG | 0.555236 | 6.4:-207775 | MS.gene74441:CDS |
| TGCAAATGTTGGTGCTGATG+TGG | 0.559498 | 6.4:-207455 | MS.gene74441:CDS |
| TGGATTTAGACGTTGAGCCA+CGG | 0.559998 | 6.4:+207698 | None:intergenic |
| TTACCAGCCTCCAAAGGATG+AGG | 0.600248 | 6.4:+207841 | None:intergenic |
| CGTGGAAGCAATGCAAATGT+TGG | 0.616233 | 6.4:-207466 | MS.gene74441:CDS |
| TCATCATTACCAGCCTCCAA+AGG | 0.621367 | 6.4:+207835 | None:intergenic |
| TGTTGGAGGAAGAAATGCAA+AGG | 0.632509 | 6.4:-207761 | MS.gene74441:CDS |
| GATATTGCACGTGGTATGAG+TGG | 0.632896 | 6.4:-207655 | MS.gene74441:CDS |
| TCTGTTGATAATGCTACTCG+TGG | 0.635589 | 6.4:-207484 | MS.gene74441:CDS |
| GTTGGAGGAAGAAATGCAAA+GGG | 0.639504 | 6.4:-207760 | MS.gene74441:CDS |
| AATTATACTGATATTGCACG+TGG | 0.677934 | 6.4:-207664 | MS.gene74441:CDS |
| TATGAATCTAGTAATGTCTG+AGG | 0.710672 | 6.4:-208081 | MS.gene74441:intron |
| TGCACGTGGTATGAGTGGAG+AGG | 0.729243 | 6.4:-207650 | MS.gene74441:CDS |
| GAGGCTGGTAATGATGAGAG+TGG | 0.750491 | 6.4:-207829 | MS.gene74441:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTATAATTTGAATATTTAT+TGG | + | chr6.4:207852-207871 | None:intergenic | 10.0% |
| !! | AAATATAGATTTGGATTTAA+TGG | - | chr6.4:208000-208019 | MS.gene74441:intron | 15.0% |
| !! | AATAACTGAATAAATAAGAT+AGG | + | chr6.4:207617-207636 | None:intergenic | 15.0% |
| !! | ATTAGTGTATCATAATCAAT+AGG | + | chr6.4:207737-207756 | None:intergenic | 20.0% |
| !! | TAAACATGCAAATATAGATT+TGG | - | chr6.4:207991-208010 | MS.gene74441:intron | 20.0% |
| ! | ATTTGCATGTTTAGCAAATA+CGG | + | chr6.4:207984-208003 | None:intergenic | 25.0% |
| ! | CATGTATTGCCTTTATAATT+TGG | - | chr6.4:207514-207533 | MS.gene74441:CDS | 25.0% |
| !!! | CCTTTATAATTTGGTTTGTT+GGG | - | chr6.4:207523-207542 | MS.gene74441:CDS | 25.0% |
| AATTATACTGATATTGCACG+TGG | - | chr6.4:207863-207882 | MS.gene74441:CDS | 30.0% | |
| CAGTTTAAGAAGGCAAATTA+AGG | + | chr6.4:207581-207600 | None:intergenic | 30.0% | |
| CCCAACAAACCAAATTATAA+AGG | + | chr6.4:207526-207545 | None:intergenic | 30.0% | |
| GTATCATAATCAATAGGCTT+GGG | + | chr6.4:207731-207750 | None:intergenic | 30.0% | |
| TGTATCATAATCAATAGGCT+TGG | + | chr6.4:207732-207751 | None:intergenic | 30.0% | |
| TTAAGAAGGCAAATTAAGGA+GGG | + | chr6.4:207577-207596 | None:intergenic | 30.0% | |
| TTTAAGAAGGCAAATTAAGG+AGG | + | chr6.4:207578-207597 | None:intergenic | 30.0% | |
| TTTGTTGGGATGTTCTATTT+AGG | - | chr6.4:207537-207556 | MS.gene74441:CDS | 30.0% | |
| ! | ACAAACAATCGATTCCATTA+AGG | - | chr6.4:207937-207956 | MS.gene74441:intron | 30.0% |
| !! | GCCTTTATAATTTGGTTTGT+TGG | - | chr6.4:207522-207541 | MS.gene74441:CDS | 30.0% |
| AATGCAAAGGGTAGAGTATA+TGG | - | chr6.4:207779-207798 | MS.gene74441:CDS | 35.0% | |
| ACAAGCAACACAGTTTAAGA+AGG | + | chr6.4:207591-207610 | None:intergenic | 35.0% | |
| ACACTAATGATTGAAGCTGT+TGG | - | chr6.4:207749-207768 | MS.gene74441:CDS | 35.0% | |
| ATGCAAAGGGTAGAGTATAT+GGG | - | chr6.4:207780-207799 | MS.gene74441:CDS | 35.0% | |
| !! | GTAATCGAGTGACTTGTTAT+AGG | - | chr6.4:207488-207507 | MS.gene74441:CDS | 35.0% |
| !!! | TTTGTTTTGACGAGCCTTAA+TGG | + | chr6.4:207954-207973 | None:intergenic | 35.0% |
| CTAATGATTGAAGCTGTTGG+AGG | - | chr6.4:207752-207771 | MS.gene74441:CDS | 40.0% | |
| GTTGGAGGAAGAAATGCAAA+GGG | - | chr6.4:207767-207786 | MS.gene74441:CDS | 40.0% | |
| TGTTGGAGGAAGAAATGCAA+AGG | - | chr6.4:207766-207785 | MS.gene74441:CDS | 40.0% | |
| ! | TAGAGTATATGGGCTTGGTT+TGG | - | chr6.4:207790-207809 | MS.gene74441:CDS | 40.0% |
| !! | TCTGTTGATAATGCTACTCG+TGG | - | chr6.4:208043-208062 | MS.gene74441:intron | 40.0% |
| AAGGGTAGAGTATATGGGCT+TGG | - | chr6.4:207785-207804 | MS.gene74441:CDS | 45.0% | |
| CGTGGAAGCAATGCAAATGT+TGG | - | chr6.4:208061-208080 | MS.gene74441:intron | 45.0% | |
| GATATTGCACGTGGTATGAG+TGG | - | chr6.4:207872-207891 | MS.gene74441:CDS | 45.0% | |
| TCATCATTACCAGCCTCCAA+AGG | + | chr6.4:207695-207714 | None:intergenic | 45.0% | |
| TGAGTCTCAACCTCATCCTT+TGG | - | chr6.4:207676-207695 | MS.gene74441:CDS | 45.0% | |
| TGGATTTAGACGTTGAGCCA+CGG | + | chr6.4:207832-207851 | None:intergenic | 45.0% | |
| !! | GTATATGGGCTTGGTTTGGA+TGG | - | chr6.4:207794-207813 | MS.gene74441:CDS | 45.0% |
| !! | TGCAAATGTTGGTGCTGATG+TGG | - | chr6.4:208072-208091 | MS.gene74441:intron | 45.0% |
| GGATTTAGACGTTGAGCCAC+GGG | + | chr6.4:207831-207850 | None:intergenic | 50.0% | |
| GTCTCAACCTCATCCTTTGG+AGG | - | chr6.4:207679-207698 | MS.gene74441:CDS | 50.0% | |
| TGGTATGAGTGGAGAGGTTG+AGG | - | chr6.4:207883-207902 | MS.gene74441:CDS | 50.0% | |
| TTACCAGCCTCCAAAGGATG+AGG | + | chr6.4:207689-207708 | None:intergenic | 50.0% | |
| ! | GAGGCTGGTAATGATGAGAG+TGG | - | chr6.4:207698-207717 | MS.gene74441:CDS | 50.0% |
| GAGAGGTTGAGGCGCGTTTA+AGG | - | chr6.4:207894-207913 | MS.gene74441:intron | 55.0% | |
| TGCACGTGGTATGAGTGGAG+AGG | - | chr6.4:207877-207896 | MS.gene74441:CDS | 55.0% | |
| ! | CAACCTCATCCTTTGGAGGC+TGG | - | chr6.4:207683-207702 | MS.gene74441:CDS | 55.0% |
| GATGGCAGCATGCGCACCCG+TGG | - | chr6.4:207812-207831 | MS.gene74441:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 207447 | 208102 | 207447 | ID=MS.gene74441 |
| chr6.4 | mRNA | 207447 | 208102 | 207447 | ID=MS.gene74441.t1;Parent=MS.gene74441 |
| chr6.4 | exon | 208082 | 208102 | 208082 | ID=MS.gene74441.t1.exon1;Parent=MS.gene74441.t1 |
| chr6.4 | CDS | 208082 | 208102 | 208082 | ID=cds.MS.gene74441.t1;Parent=MS.gene74441.t1 |
| chr6.4 | exon | 207447 | 207902 | 207447 | ID=MS.gene74441.t1.exon2;Parent=MS.gene74441.t1 |
| chr6.4 | CDS | 207447 | 207902 | 207447 | ID=cds.MS.gene74441.t1;Parent=MS.gene74441.t1 |
| Gene Sequence |
| Protein sequence |
