AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74570.t1 MTR_2g029510 97.267 439 10 1 1 437 1 439 0.0 861
MS.gene74570.t1 MTR_1g095165 45.572 463 197 12 9 417 1 462 1.22e-132 390
MS.gene74570.t1 MTR_0368s0010 48.805 293 118 8 157 417 6 298 4.85e-89 273
MS.gene74570.t1 MTR_1g072190 36.468 436 192 10 22 431 1 377 5.74e-76 242
MS.gene74570.t1 MTR_7g094940 38.875 391 190 12 22 394 1 360 1.89e-73 235
MS.gene74570.t1 MTR_5g017680 29.769 346 191 14 67 365 10 350 3.15e-27 112
MS.gene74570.t1 MTR_5g017670 30.571 350 180 17 67 365 16 353 2.30e-26 110
MS.gene74570.t1 MTR_1g019940 27.246 334 185 13 52 336 6 330 1.35e-23 102
MS.gene74570.t1 MTR_8g007065 27.913 369 226 17 67 400 32 395 3.98e-23 101
MS.gene74570.t1 MTR_1g019940 27.596 337 178 15 52 336 6 328 8.05e-23 100
MS.gene74570.t1 MTR_3g101410 27.322 366 214 15 84 401 36 397 1.84e-22 99.4
MS.gene74570.t1 MTR_8g007040 25.455 385 245 13 67 415 32 410 1.97e-22 99.4
MS.gene74570.t1 MTR_8g007090 28.919 370 223 15 67 400 32 397 4.06e-22 98.2
MS.gene74570.t1 MTR_3g101400 26.359 368 215 16 84 401 36 397 4.76e-21 95.1
MS.gene74570.t1 MTR_8g007095 28.610 374 219 15 67 400 32 397 8.16e-21 94.4
MS.gene74570.t1 MTR_8g007125 27.011 348 215 12 87 400 51 393 3.63e-20 92.4
MS.gene74570.t1 MTR_8g007140 28.363 342 207 14 87 400 52 383 3.77e-20 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74570.t1 AT3G54950 48.174 438 198 7 2 412 32 467 2.28e-133 393
MS.gene74570.t1 AT2G39220 47.285 442 184 7 19 412 40 480 1.26e-129 384
MS.gene74570.t1 AT4G29800 42.765 463 199 9 15 412 43 504 1.15e-112 341
MS.gene74570.t1 AT4G29800 42.457 464 200 9 15 412 43 505 1.31e-111 338
MS.gene74570.t1 AT3G63200 42.365 406 176 12 22 407 3 370 6.69e-86 268
MS.gene74570.t1 AT4G37060 30.130 385 217 18 67 403 19 399 4.34e-29 118
MS.gene74570.t1 AT2G26560 30.079 379 217 15 67 400 19 394 1.75e-26 110
MS.gene74570.t1 AT4G37070 28.385 384 220 17 67 401 19 396 1.10e-24 105
MS.gene74570.t1 AT4G37060 27.952 415 218 19 67 403 19 430 7.45e-24 103
MS.gene74570.t1 AT4G37070 28.649 370 209 17 67 387 19 382 1.33e-23 102
MS.gene74570.t1 AT4G37070 28.649 370 209 17 67 387 19 382 1.33e-23 102
MS.gene74570.t1 AT4G37050 28.032 371 209 16 87 402 54 421 2.47e-23 102
MS.gene74570.t1 AT5G43590 27.778 288 168 10 87 336 33 318 9.58e-21 94.0
MS.gene74570.t1 AT5G43590 27.778 288 168 10 87 336 100 385 1.91e-20 94.0
MS.gene74570.t1 AT4G37070 27.297 370 203 17 67 387 19 371 9.99e-20 90.9

Find 105 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACGAAATCTTCTCCATTT+TGG 0.144885 2.3:-60035523 MS.gene74570:CDS
GTTGAGTCAGTCTTATTTAA+AGG 0.208877 2.3:-60034176 MS.gene74570:CDS
TTGGAAAACAAATTCTTATT+CGG 0.286942 2.3:-60035504 MS.gene74570:CDS
GTCGGATTTGGTGGCGGTTT+CGG 0.292603 2.3:-60034222 MS.gene74570:CDS
GATTTCTTCGACGCCGTTGC+TGG 0.312723 2.3:-60035288 MS.gene74570:CDS
GGCGAAAACCGCGTCGGATT+TGG 0.323987 2.3:-60034234 MS.gene74570:CDS
TTAGACAAATTGAAATTGTT+TGG 0.328034 2.3:-60034128 MS.gene74570:CDS
GTTTGGTGGAGAGTTGATTA+AGG 0.342805 2.3:-60034111 MS.gene74570:CDS
ATGTGCGGATTCAATCAAAT+AGG 0.345854 2.3:-60034286 MS.gene74570:CDS
AATATGAGAGTTATTGTTAC+CGG 0.360900 2.3:+60035314 None:intergenic
GCAGAGAATTCTGACTTTCC+CGG 0.367032 2.3:+60035416 None:intergenic
TCAGGTTTCGTGAGAATTGC+CGG 0.370289 2.3:-60034709 MS.gene74570:CDS
AACAGAATTTCCCGACGATC+GGG 0.375905 2.3:-60035159 MS.gene74570:CDS
GAGGATGCCGTCGGTGTAAC+CGG 0.403495 2.3:+60035383 None:intergenic
GTCCTAGGAGATGAAGTTCT+AGG 0.411203 2.3:+60034026 None:intergenic
CCAACTGTTGTGTTGAAGAA+TGG 0.412565 2.3:-60034059 MS.gene74570:CDS
TTCGACGCCGTTGCTGGCTC+CGG 0.416327 2.3:-60035282 MS.gene74570:CDS
TAACGGTGTTTCTGATTTGT+TGG 0.422061 2.3:-60034782 MS.gene74570:CDS
AAGTTCAAGTTGTTGTTCAT+TGG 0.424195 2.3:+60035585 None:intergenic
TCTTTCTGAGGCAGGATTCA+AGG 0.424873 2.3:+60035340 None:intergenic
GATTTGTTGGTGCTTTCTCT+TGG 0.427963 2.3:-60034769 MS.gene74570:CDS
CAAGCTGTATCAATGGCATT+TGG 0.428031 2.3:-60034329 MS.gene74570:CDS
TTTCTCACCGGAAACAAAAT+CGG 0.433911 2.3:-60035121 MS.gene74570:CDS
ACCGGATTTCTTTCTGAGGC+AGG 0.435141 2.3:+60035332 None:intergenic
CGGTGAAGGAGCTTCCGATA+TGG 0.438606 2.3:-60034689 MS.gene74570:intron
CCCGACGATCGGGGATTCTC+CGG 0.439862 2.3:-60035149 MS.gene74570:CDS
ACGAAGATCTTAGCCGTTGA+TGG 0.448292 2.3:-60034880 MS.gene74570:CDS
GAAACCTGAGGGAGACTTAA+CGG 0.454179 2.3:+60034723 None:intergenic
CCGGAGAATCCCCGATCGTC+GGG 0.457645 2.3:+60035149 None:intergenic
TCGTAGCAGGGAATTAAAAT+CGG 0.459582 2.3:+60035045 None:intergenic
GCCGTTGCTGGCTCCGGTGT+CGG 0.460782 2.3:-60035276 MS.gene74570:CDS
GTCAGAATTCTCTGCATTGA+CGG 0.462797 2.3:-60035408 MS.gene74570:CDS
TGTGCGGATTCAATCAAATA+GGG 0.467847 2.3:-60034285 MS.gene74570:CDS
GCGCGTGACGAGATCGTAGC+AGG 0.470309 2.3:+60035032 None:intergenic
GACAAATTGAAATTGTTTGG+TGG 0.485720 2.3:-60034125 MS.gene74570:CDS
TCGATGGACTTCAATTCAAT+AGG 0.487410 2.3:+60034910 None:intergenic
TTCAATTCAATAGGAGCCGC+TGG 0.490050 2.3:+60034919 None:intergenic
GATTTCGAGCGCGTCGGCGC+GGG 0.491450 2.3:+60034993 None:intergenic
TTCTCTTGGTAACGGAGAGT+TGG 0.496512 2.3:-60034755 MS.gene74570:CDS
AACCGCGTCGGATTTGGTGG+CGG 0.497777 2.3:-60034228 MS.gene74570:CDS
ATCAAATAGGGTGATGGCTA+AGG 0.509476 2.3:-60034273 MS.gene74570:CDS
TTTACCACGGGTGAAAAGTA+AGG 0.511204 2.3:+60035239 None:intergenic
TTGGTGCTTTCTCTTGGTAA+CGG 0.511918 2.3:-60034763 MS.gene74570:CDS
TAACAGAATTTCCCGACGAT+CGG 0.515375 2.3:-60035160 MS.gene74570:CDS
GAAAACCGCGTCGGATTTGG+TGG 0.518417 2.3:-60034231 MS.gene74570:CDS
GGCGGTTTCGGAGGAAATGT+TGG 0.519934 2.3:-60034210 MS.gene74570:CDS
GTAGTAGCTGATGAAGTCCT+AGG 0.521279 2.3:+60034011 None:intergenic
CTCCTAGAACTTCATCTCCT+AGG 0.523753 2.3:-60034028 MS.gene74570:CDS
GGTTGATCAAGCTGTATCAA+TGG 0.525148 2.3:-60034336 MS.gene74570:CDS
AGTTGATTAAGGAAGAAGAG+AGG 0.528571 2.3:-60034100 MS.gene74570:CDS
TTCGTGAGAATTGCCGGTGA+AGG 0.529684 2.3:-60034703 MS.gene74570:CDS
AACGCCGTTAAGTCTCCCTC+AGG 0.530840 2.3:-60034727 MS.gene74570:CDS
AGTCAGTCTTATTTAAAGGA+AGG 0.532895 2.3:-60034172 MS.gene74570:CDS
TCCTGCCTCAGAAAGAAATC+CGG 0.534121 2.3:-60035333 MS.gene74570:CDS
CCATTCTTCAACACAACAGT+TGG 0.536757 2.3:+60034059 None:intergenic
CATATCGGAAGCTCCTTCAC+CGG 0.540361 2.3:+60034690 None:intergenic
GCCGGAGAATCCCCGATCGT+CGG 0.540416 2.3:+60035148 None:intergenic
GGCGAGGACTCCACCGACAC+CGG 0.540476 2.3:+60035263 None:intergenic
ATTCTCTGCATTGACGGCGC+CGG 0.540611 2.3:-60035402 MS.gene74570:CDS
GATCTTCGTCTTCCCATCGA+TGG 0.541076 2.3:+60034894 None:intergenic
GAGTTTATCAACTTCGAAGT+TGG 0.546260 2.3:+60035548 None:intergenic
TCAGAAACACCGTTACAAAA+CGG 0.547389 2.3:+60034790 None:intergenic
CGATTTCGAGCGCGTCGGCG+CGG 0.551437 2.3:+60034992 None:intergenic
GGAGATGAAGTTCTAGGAGA+GGG 0.565558 2.3:+60034032 None:intergenic
TGAGTAACAGCAGTAGCCGT+TGG 0.567616 2.3:+60034838 None:intergenic
GTTGCTGGCTCCGGTGTCGG+TGG 0.572413 2.3:-60035273 MS.gene74570:CDS
ACCGACACCGGAGCCAGCAA+CGG 0.573642 2.3:+60035275 None:intergenic
CAGAAACTTCAAAGCTTCAT+CGG 0.575680 2.3:+60035194 None:intergenic
GGATTTGGTGGCGGTTTCGG+AGG 0.577646 2.3:-60034219 MS.gene74570:CDS
ATTGAATTGAAGTCCATCGA+TGG 0.577788 2.3:-60034907 MS.gene74570:CDS
AGCCGCTGGATCAGCTGACG+TGG 0.581310 2.3:+60034933 None:intergenic
GACGGCGCCGGTTACACCGA+CGG 0.582223 2.3:-60035390 MS.gene74570:CDS
AGGAGATGAAGTTCTAGGAG+AGG 0.585774 2.3:+60034031 None:intergenic
AAGGTGAGAGAGTGATTTCG+CGG 0.586435 2.3:+60035359 None:intergenic
CATCGCAACACCTCCATCAA+CGG 0.587463 2.3:+60034867 None:intergenic
GATTCAATCAAATAGGGTGA+TGG 0.589145 2.3:-60034279 MS.gene74570:CDS
TTGAATTGAAGTCCATCGAT+GGG 0.595371 2.3:-60034906 MS.gene74570:CDS
AGGTAATCCGTCTTTACCAC+GGG 0.595561 2.3:+60035227 None:intergenic
ACAGAATTTCCCGACGATCG+GGG 0.599858 2.3:-60035158 MS.gene74570:CDS
ATCGTCGGGAAATTCTGTTA+CGG 0.601025 2.3:+60035163 None:intergenic
TAAGGTAAACAAAGGGAAGA+CGG 0.617337 2.3:-60034255 MS.gene74570:CDS
TGTTACCGGATTTCTTTCTG+AGG 0.623207 2.3:+60035328 None:intergenic
TGCCACGTCAGCTGATCCAG+CGG 0.628488 2.3:-60034935 MS.gene74570:CDS
CGCGTGACGAGATCGTAGCA+GGG 0.629024 2.3:+60035033 None:intergenic
GTGATGGCTAAGGTAAACAA+AGG 0.633073 2.3:-60034263 MS.gene74570:CDS
AAGATCTTAGCCGTTGATGG+AGG 0.638110 2.3:-60034877 MS.gene74570:CDS
TTTCGCGGCGAGGATGCCGT+CGG 0.641184 2.3:+60035374 None:intergenic
GCAATTCTCACGAAACCTGA+GGG 0.641382 2.3:+60034712 None:intergenic
GTAGATTGAACAATTATGTG+CGG 0.642262 2.3:-60034301 MS.gene74570:CDS
GCCGACGCGCTCGAAATCGA+CGG 0.644027 2.3:-60034988 MS.gene74570:CDS
TGATGGCTAAGGTAAACAAA+GGG 0.644686 2.3:-60034262 MS.gene74570:CDS
GGCAATTCTCACGAAACCTG+AGG 0.645971 2.3:+60034711 None:intergenic
GAGGTAATCCGTCTTTACCA+CGG 0.646558 2.3:+60035226 None:intergenic
ACCGTCGATTTCGAGCGCGT+CGG 0.648949 2.3:+60034987 None:intergenic
ATTTAAAGGAAGGAAGATCG+TGG 0.667169 2.3:-60034162 MS.gene74570:CDS
TCGGCGCGGGAGAAAACGAA+CGG 0.671166 2.3:+60035006 None:intergenic
GGGTGAAAAGTAAGGCGGCG+AGG 0.686118 2.3:+60035247 None:intergenic
GCTTCATCGGCAGTGAACAG+AGG 0.690278 2.3:+60035207 None:intergenic
TTTCCGGTGAGAAAACTCGC+CGG 0.691056 2.3:+60035130 None:intergenic
TGTTGCGATGAACAATCCAA+CGG 0.695987 2.3:-60034854 MS.gene74570:CDS
ACCACGGGTGAAAAGTAAGG+CGG 0.698481 2.3:+60035242 None:intergenic
GAGAGAGTGATTTCGCGGCG+AGG 0.707647 2.3:+60035364 None:intergenic
GAAGACGGCGAAAACCGCGT+CGG 0.715374 2.3:-60034240 MS.gene74570:CDS
GAGAATTCTGACTTTCCCGG+CGG 0.726321 2.3:+60035419 None:intergenic
AACCGCCACCAAATCCGACG+CGG 0.727539 2.3:+60034226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTCGAAATTGAAT+CGG - chr2.3:60035047-60035066 MS.gene74570:CDS 15.0%
!! ATGAATGAATATTATAATGT+AGG - chr2.3:60035232-60035251 MS.gene74570:CDS 15.0%
!! AATTTGTAGTAATAATGAGT+AGG - chr2.3:60035156-60035175 MS.gene74570:CDS 20.0%
!! TTAGACAAATTGAAATTGTT+TGG - chr2.3:60035461-60035480 MS.gene74570:CDS 20.0%
!! TTGGAAAACAAATTCTTATT+CGG - chr2.3:60034085-60034104 MS.gene74570:CDS 20.0%
!!! GAATTTTTTTCTTACCATAT+CGG + chr2.3:60034917-60034936 None:intergenic 20.0%
!!! TAAGAATTTGTTTTCCAAAA+TGG + chr2.3:60034083-60034102 None:intergenic 20.0%
! AATATGAGAGTTATTGTTAC+CGG + chr2.3:60034278-60034297 None:intergenic 25.0%
! TAATGAGTAGGTCTAATTAT+TGG - chr2.3:60035168-60035187 MS.gene74570:CDS 25.0%
!!! AGAATGTGTTTGTTTTTGTT+AGG + chr2.3:60035109-60035128 None:intergenic 25.0%
ATAATTCGAAATTGAATCGG+TGG - chr2.3:60035050-60035069 MS.gene74570:CDS 30.0%
GACAAATTGAAATTGTTTGG+TGG - chr2.3:60035464-60035483 MS.gene74570:CDS 30.0%
GTAGATTGAACAATTATGTG+CGG - chr2.3:60035288-60035307 MS.gene74570:CDS 30.0%
! AAGCTTTTCCGAAAAACATT+CGG - chr2.3:60034493-60034512 MS.gene74570:intron 30.0%
! ATACGAAATCTTCTCCATTT+TGG - chr2.3:60034066-60034085 MS.gene74570:CDS 30.0%
!! AAGTTCAAGTTGTTGTTCAT+TGG + chr2.3:60034007-60034026 None:intergenic 30.0%
!! AGTCAGTCTTATTTAAAGGA+AGG - chr2.3:60035417-60035436 MS.gene74570:CDS 30.0%
!! GTTGAGTCAGTCTTATTTAA+AGG - chr2.3:60035413-60035432 MS.gene74570:CDS 30.0%
AGTTGATTAAGGAAGAAGAG+AGG - chr2.3:60035489-60035508 MS.gene74570:CDS 35.0%
ATGTGCGGATTCAATCAAAT+AGG - chr2.3:60035303-60035322 MS.gene74570:CDS 35.0%
ATTGAATTGAAGTCCATCGA+TGG - chr2.3:60034682-60034701 MS.gene74570:intron 35.0%
ATTTAAAGGAAGGAAGATCG+TGG - chr2.3:60035427-60035446 MS.gene74570:CDS 35.0%
CAGAAACTTCAAAGCTTCAT+CGG + chr2.3:60034398-60034417 None:intergenic 35.0%
GAGTTTATCAACTTCGAAGT+TGG + chr2.3:60034044-60034063 None:intergenic 35.0%
GATTCAATCAAATAGGGTGA+TGG - chr2.3:60035310-60035329 MS.gene74570:CDS 35.0%
TAAGGTAAACAAAGGGAAGA+CGG - chr2.3:60035334-60035353 MS.gene74570:CDS 35.0%
TCAGAAACACCGTTACAAAA+CGG + chr2.3:60034802-60034821 None:intergenic 35.0%
TCGTAGCAGGGAATTAAAAT+CGG + chr2.3:60034547-60034566 None:intergenic 35.0%
TGATGGCTAAGGTAAACAAA+GGG - chr2.3:60035327-60035346 MS.gene74570:CDS 35.0%
TGTGCGGATTCAATCAAATA+GGG - chr2.3:60035304-60035323 MS.gene74570:CDS 35.0%
TTGAATTGAAGTCCATCGAT+GGG - chr2.3:60034683-60034702 MS.gene74570:intron 35.0%
TTTCTCACCGGAAACAAAAT+CGG - chr2.3:60034468-60034487 MS.gene74570:intron 35.0%
! AAGCGAATTCTTCGCATTTT+CGG + chr2.3:60034122-60034141 None:intergenic 35.0%
! CATGAGTTTCCGTTTTGTAA+CGG - chr2.3:60034790-60034809 MS.gene74570:CDS 35.0%
! TAACGGTGTTTCTGATTTGT+TGG - chr2.3:60034807-60034826 MS.gene74570:CDS 35.0%
! TCGATGGACTTCAATTCAAT+AGG + chr2.3:60034682-60034701 None:intergenic 35.0%
! TGCATTCTCCGAATGTTTTT+CGG + chr2.3:60034504-60034523 None:intergenic 35.0%
ATCGTCGGGAAATTCTGTTA+CGG + chr2.3:60034429-60034448 None:intergenic 40.0%
CAAGCTGTATCAATGGCATT+TGG - chr2.3:60035260-60035279 MS.gene74570:CDS 40.0%
CCAACTGTTGTGTTGAAGAA+TGG - chr2.3:60035530-60035549 MS.gene74570:CDS 40.0%
CCATTCTTCAACACAACAGT+TGG + chr2.3:60035533-60035552 None:intergenic 40.0%
GGTTGATCAAGCTGTATCAA+TGG - chr2.3:60035253-60035272 MS.gene74570:CDS 40.0%
GTCAGAATTCTCTGCATTGA+CGG - chr2.3:60034181-60034200 MS.gene74570:CDS 40.0%
GTGATGGCTAAGGTAAACAA+AGG - chr2.3:60035326-60035345 MS.gene74570:CDS 40.0%
TAACAGAATTTCCCGACGAT+CGG - chr2.3:60034429-60034448 MS.gene74570:intron 40.0%
TGAATCGGTGGTTACTAGTT+AGG - chr2.3:60035062-60035081 MS.gene74570:CDS 40.0%
TGTTACCGGATTTCTTTCTG+AGG + chr2.3:60034264-60034283 None:intergenic 40.0%
TGTTGCGATGAACAATCCAA+CGG - chr2.3:60034735-60034754 MS.gene74570:CDS 40.0%
TTTACCACGGGTGAAAAGTA+AGG + chr2.3:60034353-60034372 None:intergenic 40.0%
! ATCAAATAGGGTGATGGCTA+AGG - chr2.3:60035316-60035335 MS.gene74570:CDS 40.0%
! GTTTGGTGGAGAGTTGATTA+AGG - chr2.3:60035478-60035497 MS.gene74570:CDS 40.0%
! TTGGTGCTTTCTCTTGGTAA+CGG - chr2.3:60034826-60034845 MS.gene74570:CDS 40.0%
!! AGCTTCTCCGATTTTGTTTC+CGG + chr2.3:60034478-60034497 None:intergenic 40.0%
!! ATTCTTCGCATTTTCGGTGT+CGG + chr2.3:60034116-60034135 None:intergenic 40.0%
!! GATTTGTTGGTGCTTTCTCT+TGG - chr2.3:60034820-60034839 MS.gene74570:CDS 40.0%
AACAGAATTTCCCGACGATC+GGG - chr2.3:60034430-60034449 MS.gene74570:intron 45.0%
AAGGTGAGAGAGTGATTTCG+CGG + chr2.3:60034233-60034252 None:intergenic 45.0%
ACGAAGATCTTAGCCGTTGA+TGG - chr2.3:60034709-60034728 MS.gene74570:CDS 45.0%
AGGTAATCCGTCTTTACCAC+GGG + chr2.3:60034365-60034384 None:intergenic 45.0%
CTCCTAGAACTTCATCTCCT+AGG - chr2.3:60035561-60035580 MS.gene74570:CDS 45.0%
GAAACCTGAGGGAGACTTAA+CGG + chr2.3:60034869-60034888 None:intergenic 45.0%
GAGGTAATCCGTCTTTACCA+CGG + chr2.3:60034366-60034385 None:intergenic 45.0%
GCAATTCTCACGAAACCTGA+GGG + chr2.3:60034880-60034899 None:intergenic 45.0%
GCAGAGAATTCTGACTTTCC+CGG + chr2.3:60034176-60034195 None:intergenic 45.0%
GTAGTAGCTGATGAAGTCCT+AGG + chr2.3:60035581-60035600 None:intergenic 45.0%
GTCCTAGGAGATGAAGTTCT+AGG + chr2.3:60035566-60035585 None:intergenic 45.0%
TCAGGTTTCGTGAGAATTGC+CGG - chr2.3:60034880-60034899 MS.gene74570:CDS 45.0%
TCCTGCCTCAGAAAGAAATC+CGG - chr2.3:60034256-60034275 MS.gene74570:CDS 45.0%
TTCAATTCAATAGGAGCCGC+TGG + chr2.3:60034673-60034692 None:intergenic 45.0%
! AAGATCTTAGCCGTTGATGG+AGG - chr2.3:60034712-60034731 MS.gene74570:CDS 45.0%
! CTTTTCACCCGTGGTAAAGA+CGG - chr2.3:60034355-60034374 MS.gene74570:intron 45.0%
!! AGGAGATGAAGTTCTAGGAG+AGG + chr2.3:60035561-60035580 None:intergenic 45.0%
!! GGAGATGAAGTTCTAGGAGA+GGG + chr2.3:60035560-60035579 None:intergenic 45.0%
!! TCTTTCTGAGGCAGGATTCA+AGG + chr2.3:60034252-60034271 None:intergenic 45.0%
!! TTCTCTTGGTAACGGAGAGT+TGG - chr2.3:60034834-60034853 MS.gene74570:CDS 45.0%
AAACAAGCCAAAAACGCCGC+CGG - chr2.3:60034154-60034173 MS.gene74570:CDS 50.0%
ACAGAATTTCCCGACGATCG+GGG - chr2.3:60034431-60034450 MS.gene74570:intron 50.0%
ACCACGGGTGAAAAGTAAGG+CGG + chr2.3:60034350-60034369 None:intergenic 50.0%
CATATCGGAAGCTCCTTCAC+CGG + chr2.3:60034902-60034921 None:intergenic 50.0%
CATCGCAACACCTCCATCAA+CGG + chr2.3:60034725-60034744 None:intergenic 50.0%
GAGAATTCTGACTTTCCCGG+CGG + chr2.3:60034173-60034192 None:intergenic 50.0%
GATCTTCGTCTTCCCATCGA+TGG + chr2.3:60034698-60034717 None:intergenic 50.0%
GGCAATTCTCACGAAACCTG+AGG + chr2.3:60034881-60034900 None:intergenic 50.0%
TGAGTAACAGCAGTAGCCGT+TGG + chr2.3:60034754-60034773 None:intergenic 50.0%
TTCGTGAGAATTGCCGGTGA+AGG - chr2.3:60034886-60034905 MS.gene74570:CDS 50.0%
TTTCCGGTGAGAAAACTCGC+CGG + chr2.3:60034462-60034481 None:intergenic 50.0%
!! ACCGGATTTCTTTCTGAGGC+AGG + chr2.3:60034260-60034279 None:intergenic 50.0%
AACAAGCCAAAAACGCCGCC+GGG - chr2.3:60034155-60034174 MS.gene74570:CDS 55.0%
AACGCCGTTAAGTCTCCCTC+AGG - chr2.3:60034862-60034881 MS.gene74570:CDS 55.0%
CGGTGAAGGAGCTTCCGATA+TGG - chr2.3:60034900-60034919 MS.gene74570:CDS 55.0%
GAAAACCGCGTCGGATTTGG+TGG - chr2.3:60035358-60035377 MS.gene74570:CDS 55.0%
GATTTCTTCGACGCCGTTGC+TGG - chr2.3:60034301-60034320 MS.gene74570:CDS 55.0%
GCTTCATCGGCAGTGAACAG+AGG + chr2.3:60034385-60034404 None:intergenic 55.0%
GGCGGTTTCGGAGGAAATGT+TGG - chr2.3:60035379-60035398 MS.gene74570:CDS 55.0%
! ATTCTCTGCATTGACGGCGC+CGG - chr2.3:60034187-60034206 MS.gene74570:CDS 55.0%
! TCTCCGGCGAGTTTTCTCAC+CGG - chr2.3:60034456-60034475 MS.gene74570:intron 55.0%
!! GTCGGATTTGGTGGCGGTTT+CGG - chr2.3:60035367-60035386 MS.gene74570:CDS 55.0%
AACCGCCACCAAATCCGACG+CGG + chr2.3:60035366-60035385 None:intergenic 60.0%
CGCGTGACGAGATCGTAGCA+GGG + chr2.3:60034559-60034578 None:intergenic 60.0%
GAAGACGGCGAAAACCGCGT+CGG - chr2.3:60035349-60035368 MS.gene74570:CDS 60.0%
GAGAGAGTGATTTCGCGGCG+AGG + chr2.3:60034228-60034247 None:intergenic 60.0%
GAGGATGCCGTCGGTGTAAC+CGG + chr2.3:60034209-60034228 None:intergenic 60.0%
GGCGAAAACCGCGTCGGATT+TGG - chr2.3:60035355-60035374 MS.gene74570:CDS 60.0%
GGGTGAAAAGTAAGGCGGCG+AGG + chr2.3:60034345-60034364 None:intergenic 60.0%
TCGGCGCGGGAGAAAACGAA+CGG + chr2.3:60034586-60034605 None:intergenic 60.0%
TGCCACGTCAGCTGATCCAG+CGG - chr2.3:60034654-60034673 MS.gene74570:intron 60.0%
! GACTTTCCCGGCGGCGTTTT+TGG + chr2.3:60034164-60034183 None:intergenic 60.0%
!! AACCGCGTCGGATTTGGTGG+CGG - chr2.3:60035361-60035380 MS.gene74570:CDS 60.0%
!! ACCGTCGATTTCGAGCGCGT+CGG + chr2.3:60034605-60034624 None:intergenic 60.0%
!! GCCGCCTTACTTTTCACCCG+TGG - chr2.3:60034346-60034365 MS.gene74570:intron 60.0%
!! GGATTTGGTGGCGGTTTCGG+AGG - chr2.3:60035370-60035389 MS.gene74570:CDS 60.0%
ACCGACACCGGAGCCAGCAA+CGG + chr2.3:60034317-60034336 None:intergenic 65.0%
AGCCGCTGGATCAGCTGACG+TGG + chr2.3:60034659-60034678 None:intergenic 65.0%
CCCGACGATCGGGGATTCTC+CGG - chr2.3:60034440-60034459 MS.gene74570:intron 65.0%
CCGGAGAATCCCCGATCGTC+GGG + chr2.3:60034443-60034462 None:intergenic 65.0%
GCCGACGCGCTCGAAATCGA+CGG - chr2.3:60034601-60034620 MS.gene74570:intron 65.0%
GCCGGAGAATCCCCGATCGT+CGG + chr2.3:60034444-60034463 None:intergenic 65.0%
GCGCGTGACGAGATCGTAGC+AGG + chr2.3:60034560-60034579 None:intergenic 65.0%
TTTCGCGGCGAGGATGCCGT+CGG + chr2.3:60034218-60034237 None:intergenic 65.0%
!! TTCGACGCCGTTGCTGGCTC+CGG - chr2.3:60034307-60034326 MS.gene74570:CDS 65.0%
GGCGAGGACTCCACCGACAC+CGG + chr2.3:60034329-60034348 None:intergenic 70.0%
! CGATTTCGAGCGCGTCGGCG+CGG + chr2.3:60034600-60034619 None:intergenic 70.0%
! GATTTCGAGCGCGTCGGCGC+GGG + chr2.3:60034599-60034618 None:intergenic 70.0%
!! GACGGCGCCGGTTACACCGA+CGG - chr2.3:60034199-60034218 MS.gene74570:CDS 70.0%
!! GCCGTTGCTGGCTCCGGTGT+CGG - chr2.3:60034313-60034332 MS.gene74570:CDS 70.0%
!! GTTGCTGGCTCCGGTGTCGG+TGG - chr2.3:60034316-60034335 MS.gene74570:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 60033983 60035628 60033983 ID=MS.gene74570
chr2.3 mRNA 60033983 60035628 60033983 ID=MS.gene74570.t1;Parent=MS.gene74570
chr2.3 exon 60034690 60035628 60034690 ID=MS.gene74570.t1.exon1;Parent=MS.gene74570.t1
chr2.3 CDS 60034690 60035628 60034690 ID=cds.MS.gene74570.t1;Parent=MS.gene74570.t1
chr2.3 exon 60033983 60034357 60033983 ID=MS.gene74570.t1.exon2;Parent=MS.gene74570.t1
chr2.3 CDS 60033983 60034357 60033983 ID=cds.MS.gene74570.t1;Parent=MS.gene74570.t1
Gene Sequence

>MS.gene74570

ATGGCGGCACTGTCAACATCACCAATGAACAACAACTTGAACTTGAACACAATTGACACCAACTTCGAAGTTGATAAACTCACATACGAAATCTTCTCCATTTTGGAAAACAAATTCTTATTCGGATACACCGACACCGAAAATGCGAAGAATTCGCTTTCGATTGATGTCAAACAAGCCAAAAACGCCGCCGGGAAAGTCAGAATTCTCTGCATTGACGGCGCCGGTTACACCGACGGCATCCTCGCCGCGAAATCACTCTCTCACCTTGAATCCTGCCTCAGAAAGAAATCCGGTAACAATAACTCTCATATTGCTGATTTCTTCGACGCCGTTGCTGGCTCCGGTGTCGGTGGAGTCCTCGCCGCCTTACTTTTCACCCGTGGTAAAGACGGATTACCTCTGTTCACTGCCGATGAAGCTTTGAAGTTTCTGATCAACAACCGTAACAGAATTTCCCGACGATCGGGGATTCTCCGGCGAGTTTTCTCACCGGAAACAAAATCGGAGAAGCTTTTCCGAAAAACATTCGGAGAATGCACACTGAAAGACACATTGAAACCGATTTTAATTCCCTGCTACGATCTCGTCACGCGCGCACCGTTCGTTTTCTCCCGCGCCGACGCGCTCGAAATCGACGGTTACGATTTCAAGATGCGTGATGTCTGTGCTGCCACGTCAGCTGATCCAGCGGCTCCTATTGAATTGAAGTCCATCGATGGGAAGACGAAGATCTTAGCCGTTGATGGAGGTGTTGCGATGAACAATCCAACGGCTACTGCTGTTACTCACGTGCTTAATAATAAACATGAGTTTCCGTTTTGTAACGGTGTTTCTGATTTGTTGGTGCTTTCTCTTGGTAACGGAGAGTTGGACTTTAACGCCGTTAAGTCTCCCTCAGGTTTCGTGAGAATTGCCGGTGAAGGAGCTTCCGATATGGTAAGAAAAAAATTCTTAAAAAAATAATCAATTATTGTTTTTCAATATTTAAATAAATCGAATACGACAAAGAAAATAAAGTTGCGTATCTTAAATTGTGATTTTTTTTATTAATTTAATTAATTAAAATAATTCGAAATTGAATCGGTGGTTACTAGTTAGGCTAAGAGTAATGAATTTGTCACCTAACAAAAACAAACACATTCTTTTATATTTATTTATTTAAAAAATAATAATTTGTAGTAATAATGAGTAGGTCTAATTATTGGCATGTATATATTTTTATTCTTATGAATAATGTGACTCATGTATGAATGAATATTATAATGTAGGTTGATCAAGCTGTATCAATGGCATTTGGAGAATGTAGATTGAACAATTATGTGCGGATTCAATCAAATAGGGTGATGGCTAAGGTAAACAAAGGGAAGACGGCGAAAACCGCGTCGGATTTGGTGGCGGTTTCGGAGGAAATGTTGGCACAGAAGAATGTTGAGTCAGTCTTATTTAAAGGAAGGAAGATCGTGGAGAACACAAATTTAGACAAATTGAAATTGTTTGGTGGAGAGTTGATTAAGGAAGAAGAGAGGAGAAAAACTAGTATTTTACCAACTGTTGTGTTGAAGAATGGATCTCCCTCTCCTAGAACTTCATCTCCTAGGACTTCATCAGCTACTACTTTGTCAACTTCATCTTCAAGCTCTTAG
Protein sequence

>MS.gene74570.t1

MAALSTSPMNNNLNLNTIDTNFEVDKLTYEIFSILENKFLFGYTDTENAKNSLSIDVKQAKNAAGKVRILCIDGAGYTDGILAAKSLSHLESCLRKKSGNNNSHIADFFDAVAGSGVGGVLAALLFTRGKDGLPLFTADEALKFLINNRNRISRRSGILRRVFSPETKSEKLFRKTFGECTLKDTLKPILIPCYDLVTRAPFVFSRADALEIDGYDFKMRDVCAATSADPAAPIELKSIDGKTKILAVDGGVAMNNPTATAVTHVLNNKHEFPFCNGVSDLLVLSLGNGELDFNAVKSPSGFVRIAGEGASDMVDQAVSMAFGECRLNNYVRIQSNRVMAKVNKGKTAKTASDLVAVSEEMLAQKNVESVLFKGRKIVENTNLDKLKLFGGELIKEEERRKTSILPTVVLKNGSPSPRTSSPRTSSATTLSTSSSSS