AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055616


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055616.t1 MTR_1g054285 84.804 204 3 4 1 176 1 204 3.37e-114 326
MS.gene055616.t1 MTR_7g093430 54.028 211 61 7 1 176 1 210 7.03e-58 183
MS.gene055616.t1 MTR_4g100550 54.167 120 44 2 59 176 60 170 3.95e-33 119
MS.gene055616.t1 MTR_5g013010 52.672 131 41 4 54 176 69 186 9.97e-33 116
MS.gene055616.t1 MTR_5g013010 53.968 126 37 4 59 176 74 186 1.01e-31 115
MS.gene055616.t1 MTR_2g061030 42.000 250 71 10 1 176 1 250 2.83e-31 115
MS.gene055616.t1 MTR_4g097600 80.000 65 11 1 114 176 113 177 8.50e-29 107
MS.gene055616.t1 MTR_8g006705 54.054 111 45 1 72 176 86 196 6.03e-26 100
MS.gene055616.t1 MTR_5g014890 75.410 61 14 1 117 176 113 173 5.30e-24 95.1
MS.gene055616.t1 MTR_1g017090 46.341 123 51 4 61 176 66 180 2.18e-21 88.6
MS.gene055616.t1 MTR_3g103590 68.333 60 19 0 117 176 136 195 3.34e-21 88.2
MS.gene055616.t1 MTR_4g084750 73.684 57 14 1 121 176 59 115 2.75e-20 84.0
MS.gene055616.t1 MTR_4g126900 69.388 49 15 0 128 176 90 138 3.52e-17 76.3
MS.gene055616.t1 MTR_2g038625 57.353 68 28 1 109 176 66 132 1.75e-16 74.3
MS.gene055616.t1 MTR_2g038580 57.353 68 28 1 109 176 66 132 1.75e-16 74.3
MS.gene055616.t1 MTR_4g107650 50.602 83 37 2 94 176 67 145 2.20e-14 70.1
MS.gene055616.t1 MTR_7g010020 42.391 92 52 1 85 176 40 130 2.97e-14 68.9
MS.gene055616.t1 MTR_8g089895 43.617 94 49 2 83 176 15 104 8.59e-13 65.5
MS.gene055616.t1 MTR_5g038280 48.611 72 34 1 105 176 69 137 1.04e-12 65.1
MS.gene055616.t1 MTR_3g086790 40.196 102 59 2 76 176 10 110 2.46e-12 63.9
MS.gene055616.t1 MTR_6g007647 57.407 54 23 0 123 176 30 83 2.69e-12 63.5
MS.gene055616.t1 MTR_6g007647 57.407 54 23 0 123 176 64 117 3.37e-12 63.5
MS.gene055616.t1 MTR_1g061660 50.909 55 27 0 122 176 55 109 4.01e-12 62.8
MS.gene055616.t1 MTR_8g469430 54.902 51 23 0 126 176 89 139 1.77e-11 61.6
MS.gene055616.t1 MTR_5g039000 50.847 59 29 0 118 176 69 127 2.85e-11 60.8
MS.gene055616.t1 MTR_7g103340 48.276 58 30 0 119 176 56 113 3.54e-11 60.1
MS.gene055616.t1 MTR_6g011610 41.346 104 54 3 79 176 44 146 8.04e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055616.t1 AT5G06710 42.324 241 73 5 1 176 1 240 4.77e-41 139
MS.gene055616.t1 AT5G06710 42.324 241 73 5 1 176 1 240 6.50e-41 140
MS.gene055616.t1 AT5G47370 55.118 127 47 5 59 176 55 180 2.16e-32 117
MS.gene055616.t1 AT4G17460 42.347 196 71 7 3 176 10 185 4.20e-30 111
MS.gene055616.t1 AT3G60390 51.825 137 44 6 59 176 79 212 6.45e-30 111
MS.gene055616.t1 AT4G16780 56.198 121 38 3 59 176 71 179 3.56e-27 103
MS.gene055616.t1 AT2G44910 49.624 133 44 4 60 176 88 213 1.61e-26 102
MS.gene055616.t1 AT2G22800 76.667 60 14 0 117 176 104 163 2.44e-25 99.0
MS.gene055616.t1 AT4G37790 76.667 60 14 0 117 176 117 176 7.28e-25 97.8
MS.gene055616.t1 AT5G06710 36.279 215 71 5 1 150 1 214 3.04e-21 87.4
MS.gene055616.t1 AT5G06710 36.279 215 71 5 1 150 1 214 3.04e-21 87.4
MS.gene055616.t1 AT2G01430 60.294 68 27 0 109 176 122 189 7.00e-21 85.9
MS.gene055616.t1 AT2G01430 60.294 68 27 0 109 176 122 189 2.60e-20 85.5
MS.gene055616.t1 AT1G70920 65.079 63 20 1 116 176 27 89 2.04e-17 75.9
MS.gene055616.t1 AT1G70920 65.079 63 20 1 116 176 57 119 3.48e-17 75.9
MS.gene055616.t1 AT2G36610 45.312 64 27 1 121 176 66 129 3.07e-11 59.7
MS.gene055616.t1 AT3G01470 56.863 51 22 0 126 176 68 118 3.42e-11 60.5

Find 34 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAAGAGATAGGTAATTT+TGG 0.264059 1.2:+41143081 None:intergenic
AGGAATAACAATGCTGAATA+TGG 0.265099 1.2:-41142375 MS.gene055616:CDS
GGAAGTGATGATGATGAAAA+TGG 0.308043 1.2:-41142312 MS.gene055616:CDS
ATGGAACTTGCTTTGAGCTT+AGG 0.368374 1.2:-41143111 MS.gene055616:CDS
CTTGGTAGTGGATCTTTGTT+GGG 0.374601 1.2:+41143051 None:intergenic
GGAAGAAGGTCTAGTTGAAT+AGG 0.392211 1.2:+41142970 None:intergenic
GTCCTCGTCAAGTTGAAGTT+TGG 0.396562 1.2:-41142072 MS.gene055616:CDS
ACTTGGTAGTGGATCTTTGT+TGG 0.408944 1.2:+41143050 None:intergenic
CTTCGGTCGTCGCCGGAAAA+CGG 0.438226 1.2:+41142469 None:intergenic
TTGCAATGGATTTCAGTATG+AGG 0.457926 1.2:-41142395 MS.gene055616:CDS
TTCTGTGTCACCATTTGCAA+TGG 0.477662 1.2:-41142409 MS.gene055616:CDS
ATACTGAAATCCATTGCAAA+TGG 0.496930 1.2:+41142399 None:intergenic
GATCTAAGAACAGGAGAGAA+AGG 0.503681 1.2:+41142949 None:intergenic
TCGACGTCTTCGGTCGTCGC+CGG 0.507773 1.2:+41142462 None:intergenic
GGTACCGTCGTCGACGTCTT+CGG 0.509499 1.2:+41142452 None:intergenic
AGAAACAAGAGAGAAAATGA+AGG 0.510237 1.2:-41142348 MS.gene055616:CDS
GGTTTGTCAAGAAAAGAGAT+AGG 0.515910 1.2:+41143072 None:intergenic
AATGAAGGTGAAGCTGAAAG+AGG 0.521199 1.2:-41142333 MS.gene055616:CDS
GAAGTGATGATGATGAAAAT+GGG 0.525282 1.2:-41142311 MS.gene055616:CDS
AAATGGTGACACAGAACTGC+TGG 0.531470 1.2:+41142416 None:intergenic
TGTTGGGTTGAGAAGAAACT+TGG 0.533644 1.2:+41143033 None:intergenic
ATGATGAAAATGGGTCAACT+AGG 0.551419 1.2:-41142302 MS.gene055616:CDS
GAACAGGAGAGAAAGGAAGA+AGG 0.553836 1.2:+41142956 None:intergenic
GTTGAGAAGAAACTTGGTAG+TGG 0.575951 1.2:+41143039 None:intergenic
GAAATTTACAGGATTAAGAG+TGG 0.579010 1.2:+41142218 None:intergenic
AGAAGGTCTAGTTGAATAGG+TGG 0.598462 1.2:+41142973 None:intergenic
GGTTTCAAAATAGAAGAGCA+AGG 0.607323 1.2:-41142051 None:intergenic
AATGGTGACACAGAACTGCT+GGG 0.607777 1.2:+41142417 None:intergenic
AGTTTCTTCTCAACCCAACA+AGG 0.609680 1.2:-41143030 MS.gene055616:CDS
AATAACAATGCTGAATATGG+AGG 0.617867 1.2:-41142372 MS.gene055616:CDS
AACCAAACTTCAACTTGACG+AGG 0.618703 1.2:+41142070 None:intergenic
ATGGTGACACAGAACTGCTG+GGG 0.640069 1.2:+41142418 None:intergenic
ACGACCGAAGACGTCGACGA+CGG 0.675975 1.2:-41142456 MS.gene055616:CDS
TGGTGACACAGAACTGCTGG+GGG 0.743324 1.2:+41142419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAATAAGAAATTTAC+AGG + chr1.2:41142959-41142978 None:intergenic 10.0%
!! AAATATATAAAACCATTTAG+AGG + chr1.2:41142320-41142339 None:intergenic 15.0%
!!! TGAAATGTCAATTTTTTTAA+CGG - chr1.2:41142517-41142536 MS.gene055616:intron 15.0%
!!! TTGTTTCTTCTTTTTAATTA+AGG - chr1.2:41142991-41143010 MS.gene055616:CDS 15.0%
!! ATAGTAGATAGAAAATTACA+TGG + chr1.2:41142283-41142302 None:intergenic 20.0%
!!! TTTTTGTTTTTGTTATCATC+AGG - chr1.2:41142644-41142663 MS.gene055616:intron 20.0%
! AGATAGAAAATTACATGGTT+GGG + chr1.2:41142278-41142297 None:intergenic 25.0%
! TAGATAGAAAATTACATGGT+TGG + chr1.2:41142279-41142298 None:intergenic 25.0%
!! AGAAAAGAGATAGGTAATTT+TGG + chr1.2:41142085-41142104 None:intergenic 25.0%
!! ATCATTTTCTTTCCTCTAAA+TGG - chr1.2:41142305-41142324 MS.gene055616:CDS 25.0%
!! TTAAAGTGCTTGAAAATTGT+GGG - chr1.2:41142420-41142439 MS.gene055616:CDS 25.0%
!! TTTAAAGTGCTTGAAAATTG+TGG - chr1.2:41142419-41142438 MS.gene055616:CDS 25.0%
!!! TCAATTTTTTTAACGGATCT+AGG - chr1.2:41142524-41142543 MS.gene055616:intron 25.0%
AAAACCTTGAACCAAATCTT+GGG + chr1.2:41142458-41142477 None:intergenic 30.0%
AAAGCTTCAGAAGAAAAAAG+AGG - chr1.2:41142157-41142176 MS.gene055616:intron 30.0%
AATAACAATGCTGAATATGG+AGG - chr1.2:41142791-41142810 MS.gene055616:intron 30.0%
AGAAACAAGAGAGAAAATGA+AGG - chr1.2:41142815-41142834 MS.gene055616:intron 30.0%
AGGAATAACAATGCTGAATA+TGG - chr1.2:41142788-41142807 MS.gene055616:intron 30.0%
ATACTGAAATCCATTGCAAA+TGG + chr1.2:41142767-41142786 None:intergenic 30.0%
GAAATTTACAGGATTAAGAG+TGG + chr1.2:41142948-41142967 None:intergenic 30.0%
! GAAGTGATGATGATGAAAAT+GGG - chr1.2:41142852-41142871 MS.gene055616:intron 30.0%
!!! TTGTTTTTGTTATCATCAGG+TGG - chr1.2:41142647-41142666 MS.gene055616:intron 30.0%
ATGATGAAAATGGGTCAACT+AGG - chr1.2:41142861-41142880 MS.gene055616:intron 35.0%
CAAAACCTTGAACCAAATCT+TGG + chr1.2:41142459-41142478 None:intergenic 35.0%
GGTTTGTCAAGAAAAGAGAT+AGG + chr1.2:41142094-41142113 None:intergenic 35.0%
TTGAATTTGCACCCAAGATT+TGG - chr1.2:41142444-41142463 MS.gene055616:CDS 35.0%
! GGAAGTGATGATGATGAAAA+TGG - chr1.2:41142851-41142870 MS.gene055616:intron 35.0%
! TTGCAATGGATTTCAGTATG+AGG - chr1.2:41142768-41142787 MS.gene055616:intron 35.0%
!!! TTCTGAAGCTTTTCCTTGTT+GGG + chr1.2:41142149-41142168 None:intergenic 35.0%
AACCAAACTTCAACTTGACG+AGG + chr1.2:41143096-41143115 None:intergenic 40.0%
AATGAAGGTGAAGCTGAAAG+AGG - chr1.2:41142830-41142849 MS.gene055616:intron 40.0%
AGAAGGTCTAGTTGAATAGG+TGG + chr1.2:41142193-41142212 None:intergenic 40.0%
AGTTTCTTCTCAACCCAACA+AGG - chr1.2:41142133-41142152 MS.gene055616:intron 40.0%
GATCTAAGAACAGGAGAGAA+AGG + chr1.2:41142217-41142236 None:intergenic 40.0%
GGAAGAAGGTCTAGTTGAAT+AGG + chr1.2:41142196-41142215 None:intergenic 40.0%
GTAGAAGCAAAAACTTGCAC+TGG - chr1.2:41143050-41143069 MS.gene055616:CDS 40.0%
GTTGAGAAGAAACTTGGTAG+TGG + chr1.2:41142127-41142146 None:intergenic 40.0%
TTCTGTGTCACCATTTGCAA+TGG - chr1.2:41142754-41142773 MS.gene055616:intron 40.0%
! ATGGAACTTGCTTTGAGCTT+AGG - chr1.2:41142052-41142071 MS.gene055616:CDS 40.0%
! TTTGTTATCATCAGGTGGAC+CGG - chr1.2:41142652-41142671 MS.gene055616:intron 40.0%
!! ACTTGGTAGTGGATCTTTGT+TGG + chr1.2:41142116-41142135 None:intergenic 40.0%
!! CTTGGTAGTGGATCTTTGTT+GGG + chr1.2:41142115-41142134 None:intergenic 40.0%
!! TGTTGGGTTGAGAAGAAACT+TGG + chr1.2:41142133-41142152 None:intergenic 40.0%
!!! CTTCTGAAGCTTTTCCTTGT+TGG + chr1.2:41142150-41142169 None:intergenic 40.0%
AAATGGTGACACAGAACTGC+TGG + chr1.2:41142750-41142769 None:intergenic 45.0%
AATGGTGACACAGAACTGCT+GGG + chr1.2:41142749-41142768 None:intergenic 45.0%
GAACAGGAGAGAAAGGAAGA+AGG + chr1.2:41142210-41142229 None:intergenic 45.0%
! TGCACCCAAGATTTGGTTCA+AGG - chr1.2:41142451-41142470 MS.gene055616:CDS 45.0%
!! GTCCTCGTCAAGTTGAAGTT+TGG - chr1.2:41143091-41143110 MS.gene055616:CDS 45.0%
ATGGTGACACAGAACTGCTG+GGG + chr1.2:41142748-41142767 None:intergenic 50.0%
GAATGGGGAGGTGAGAAGAA+GGG + chr1.2:41142246-41142265 None:intergenic 50.0%
GGGTGAGGAGATCTAAGAAC+AGG + chr1.2:41142226-41142245 None:intergenic 50.0%
TTACATGGTTGGGTGAGCCA+AGG + chr1.2:41142268-41142287 None:intergenic 50.0%
! GCTAGACGTGAACCGTTTTC+CGG - chr1.2:41142682-41142701 MS.gene055616:intron 50.0%
ACTGCTGGGGGATGACAATG+AGG + chr1.2:41142735-41142754 None:intergenic 55.0%
ATCATCAGGTGGACCGGTGA+GGG - chr1.2:41142658-41142677 MS.gene055616:intron 55.0%
CTTCTCACCTCCCCATTCCT+TGG - chr1.2:41142248-41142267 MS.gene055616:CDS 55.0%
GGAATGGGGAGGTGAGAAGA+AGG + chr1.2:41142247-41142266 None:intergenic 55.0%
GGTTGGGTGAGCCAAGGAAT+GGG + chr1.2:41142262-41142281 None:intergenic 55.0%
GTTGGGTGAGCCAAGGAATG+GGG + chr1.2:41142261-41142280 None:intergenic 55.0%
TATCATCAGGTGGACCGGTG+AGG - chr1.2:41142657-41142676 MS.gene055616:intron 55.0%
TGGTGACACAGAACTGCTGG+GGG + chr1.2:41142747-41142766 None:intergenic 55.0%
TGGTTGGGTGAGCCAAGGAA+TGG + chr1.2:41142263-41142282 None:intergenic 55.0%
! TTCACGTCTAGCTCCCTCAC+CGG + chr1.2:41142674-41142693 None:intergenic 55.0%
ACGACCGAAGACGTCGACGA+CGG - chr1.2:41142707-41142726 MS.gene055616:intron 60.0%
CTTCGGTCGTCGCCGGAAAA+CGG + chr1.2:41142697-41142716 None:intergenic 60.0%
GGGAGGTGAGAAGAAGGGTG+AGG + chr1.2:41142241-41142260 None:intergenic 60.0%
GGTACCGTCGTCGACGTCTT+CGG + chr1.2:41142714-41142733 None:intergenic 60.0%
GGGTGAGCCAAGGAATGGGG+AGG + chr1.2:41142258-41142277 None:intergenic 65.0%
TCGACGTCTTCGGTCGTCGC+CGG + chr1.2:41142704-41142723 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 41142052 41143133 41142052 ID=MS.gene055616
chr1.2 mRNA 41142052 41143133 41142052 ID=MS.gene055616.t1;Parent=MS.gene055616
chr1.2 exon 41142960 41143133 41142960 ID=MS.gene055616.t1.exon1;Parent=MS.gene055616.t1
chr1.2 CDS 41142960 41143133 41142960 ID=cds.MS.gene055616.t1;Parent=MS.gene055616.t1
chr1.2 exon 41142227 41142502 41142227 ID=MS.gene055616.t1.exon2;Parent=MS.gene055616.t1
chr1.2 CDS 41142227 41142502 41142227 ID=cds.MS.gene055616.t1;Parent=MS.gene055616.t1
chr1.2 exon 41142052 41142131 41142052 ID=MS.gene055616.t1.exon3;Parent=MS.gene055616.t1
chr1.2 CDS 41142052 41142131 41142052 ID=cds.MS.gene055616.t1;Parent=MS.gene055616.t1
Gene Sequence

>MS.gene055616

ATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCTATCTCTTTTCTTGACAAACCCAACAAAGATCCACTACCAAGTTTCTTCTCAACCCAACAAGGAAAAGCTTCAGAAGAAAAAAGAGGTTCTTCAGATCCACCTATTCAACTAGACCTTCTTCCTTTCTCTCCTGTTCTTAGATCTCCTCACCCTTCTTCTCACCTCCCCATTCCTTGGCTCACCCAACCATGTAATTTTCTATCTACTATAAATCATTTTCTTTCCTCTAAATGGTTTTATATATTTGTTTAATATCTAGATAAATTTGTTTAATTTTATTTTGTTTTTATAGATTTAATTTTATCTTTTTTGTTTTTTTTTTTGTTTTAAAGTGCTTGAAAATTGTGGGTTTGAATTTGCACCCAAGATTTGGTTCAAGGTTTTGCATATTCTTGACTAATGCTTATTGAAAAATATTTGTTATGAAATGTCAATTTTTTTAACGGATCTAGGTTACTTCGAGATATTAGTCTTTGCAGTTACGCGCATGAATTCATATTGTTATAAAAAAAAATTGAAAATATATTTAAAGTTGTATATACTCAATCATTTTTTGTTTTTGTTATCATCAGGTGGACCGGTGAGGGAGCTAGACGTGAACCGTTTTCCGGCGACGACCGAAGACGTCGACGACGGTACCTCATTGTCATCCCCCAGCAGTTCTGTGTCACCATTTGCAATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGAAAATGAAGGTGAAGCTGAAAGAGGAAGTGATGATGATGAAAATGGGTCAACTAGGAAGAAACTAAGACTCTCTAAAGATCAATCTGCTTTTCTTGAAGAAAGTTTCAAAGAACACACCACTCTTAATCCTGTAAATTTCTTATTTAATTTTTGTTTATTTACTTGTTTCTTCTTTTTAATTAAGGTTTATTTGTTAATTAATCATGTTTATTTATTTGCTTGTAGAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTTAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAATAGAAGAGCAAG

Protein sequence

>MS.gene055616.t1

MELALSLGDTPKLPISFLDKPNKDPLPSFFSTQQGKASEEKRGSSDPPIQLDLLPFSPLDVNRFPATTEDVDDGTSLSSPSSSVSPFAMDFSMRNNNAEYGGRNKRENEGEAERGSDDDENGSTRKKLRLSKDQSAFLEESFKEHTTLNPKQKLALAKQLNLSPRQVEVWFQNRRA