Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74939.t1 | XP_013470321.1 | 96.4 | 466 | 17 | 0 | 6 | 471 | 2 | 467 | 1.30E-275 | 958.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74939.t1 | Q09131 | 83.0 | 471 | 73 | 2 | 1 | 471 | 1 | 464 | 4.4e-245 | 848.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74939.t1 | A0A072VQW8 | 96.4 | 466 | 17 | 0 | 6 | 471 | 2 | 467 | 9.1e-276 | 958.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene74939 | MS.gene036701 | PPI |
| MS.gene74939 | MS.gene58017 | PPI |
| MS.gene74939 | MS.gene58221 | PPI |
| MS.gene74939 | MS.gene58016 | PPI |
| MS.gene74939 | MS.gene58198 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74939.t1 | MTR_1g112630 | 96.352 | 466 | 17 | 0 | 6 | 471 | 2 | 467 | 0.0 | 941 |
| MS.gene74939.t1 | MTR_4g037105 | 91.091 | 449 | 39 | 1 | 23 | 471 | 18 | 465 | 0.0 | 862 |
| MS.gene74939.t1 | MTR_0188s0020 | 84.152 | 448 | 70 | 1 | 24 | 471 | 25 | 471 | 0.0 | 800 |
| MS.gene74939.t1 | MTR_4g115670 | 59.815 | 433 | 170 | 3 | 28 | 459 | 23 | 452 | 0.0 | 543 |
| MS.gene74939.t1 | MTR_3g051240 | 42.786 | 402 | 199 | 10 | 54 | 454 | 39 | 410 | 7.37e-95 | 293 |
| MS.gene74939.t1 | MTR_5g092360 | 40.406 | 443 | 230 | 12 | 30 | 470 | 22 | 432 | 8.08e-95 | 293 |
| MS.gene74939.t1 | MTR_1g060360 | 41.414 | 396 | 204 | 10 | 60 | 454 | 46 | 414 | 9.52e-91 | 283 |
| MS.gene74939.t1 | MTR_7g104330 | 41.562 | 397 | 202 | 10 | 60 | 454 | 54 | 422 | 1.03e-90 | 283 |
| MS.gene74939.t1 | MTR_7g104360 | 39.500 | 400 | 211 | 10 | 58 | 454 | 45 | 416 | 1.40e-86 | 272 |
| MS.gene74939.t1 | MTR_6g013050 | 31.028 | 535 | 261 | 15 | 7 | 449 | 1 | 519 | 6.79e-73 | 241 |
| MS.gene74939.t1 | MTR_3g083810 | 37.215 | 395 | 173 | 9 | 115 | 450 | 143 | 521 | 1.17e-71 | 236 |
| MS.gene74939.t1 | MTR_3g083820 | 37.215 | 395 | 173 | 9 | 115 | 450 | 143 | 521 | 2.22e-70 | 233 |
| MS.gene74939.t1 | MTR_7g056403 | 35.581 | 430 | 194 | 12 | 87 | 450 | 108 | 520 | 3.67e-70 | 233 |
| MS.gene74939.t1 | MTR_6g013050 | 30.291 | 515 | 251 | 15 | 7 | 429 | 1 | 499 | 5.27e-65 | 219 |
| MS.gene74939.t1 | MTR_1g052505 | 26.196 | 397 | 225 | 15 | 52 | 397 | 183 | 562 | 1.45e-28 | 119 |
| MS.gene74939.t1 | MTR_4g037670 | 27.586 | 406 | 219 | 16 | 55 | 423 | 237 | 604 | 8.22e-28 | 117 |
| MS.gene74939.t1 | MTR_4g082930 | 24.573 | 468 | 271 | 16 | 39 | 444 | 125 | 572 | 1.65e-20 | 95.1 |
| MS.gene74939.t1 | MTR_3g074930 | 25.627 | 359 | 220 | 16 | 119 | 444 | 256 | 600 | 1.99e-19 | 91.7 |
| MS.gene74939.t1 | MTR_3g074930 | 25.627 | 359 | 220 | 16 | 119 | 444 | 249 | 593 | 2.11e-19 | 91.7 |
| MS.gene74939.t1 | MTR_5g045600 | 25.135 | 370 | 208 | 13 | 119 | 444 | 245 | 589 | 3.83e-19 | 90.9 |
| MS.gene74939.t1 | MTR_4g103520 | 25.915 | 355 | 224 | 13 | 119 | 444 | 253 | 597 | 5.03e-19 | 90.5 |
| MS.gene74939.t1 | MTR_4g103520 | 25.915 | 355 | 224 | 13 | 119 | 444 | 176 | 520 | 6.25e-19 | 90.1 |
| MS.gene74939.t1 | MTR_8g063080 | 24.054 | 370 | 212 | 15 | 119 | 444 | 238 | 582 | 4.70e-17 | 84.3 |
| MS.gene74939.t1 | MTR_4g082940 | 25.424 | 236 | 149 | 8 | 227 | 444 | 332 | 558 | 2.79e-13 | 72.4 |
| MS.gene74939.t1 | MTR_3g074970 | 22.817 | 355 | 190 | 13 | 119 | 444 | 198 | 497 | 9.84e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74939.t1 | AT2G16430 | 75.269 | 465 | 113 | 2 | 1 | 465 | 1 | 463 | 0.0 | 746 |
| MS.gene74939.t1 | AT2G27190 | 67.538 | 459 | 148 | 1 | 1 | 459 | 1 | 458 | 0.0 | 659 |
| MS.gene74939.t1 | AT1G56360 | 63.496 | 452 | 157 | 4 | 18 | 464 | 10 | 458 | 0.0 | 601 |
| MS.gene74939.t1 | AT2G16430 | 80.531 | 339 | 66 | 0 | 129 | 467 | 7 | 345 | 0.0 | 596 |
| MS.gene74939.t1 | AT4G36350 | 62.061 | 456 | 166 | 4 | 13 | 464 | 6 | 458 | 0.0 | 593 |
| MS.gene74939.t1 | AT5G34850 | 56.828 | 454 | 189 | 4 | 7 | 459 | 4 | 451 | 0.0 | 540 |
| MS.gene74939.t1 | AT2G18130 | 56.114 | 458 | 173 | 7 | 13 | 466 | 8 | 441 | 0.0 | 523 |
| MS.gene74939.t1 | AT3G46120 | 47.174 | 460 | 157 | 10 | 6 | 459 | 2 | 381 | 3.11e-135 | 395 |
| MS.gene74939.t1 | AT1G52940 | 62.195 | 246 | 85 | 6 | 44 | 286 | 1 | 241 | 1.36e-106 | 317 |
| MS.gene74939.t1 | AT3G52820 | 42.929 | 396 | 198 | 11 | 60 | 454 | 47 | 415 | 6.77e-93 | 288 |
| MS.gene74939.t1 | AT3G20500 | 42.211 | 398 | 201 | 9 | 58 | 454 | 45 | 414 | 3.02e-92 | 287 |
| MS.gene74939.t1 | AT3G52810 | 39.198 | 449 | 235 | 12 | 5 | 450 | 2 | 415 | 4.36e-89 | 279 |
| MS.gene74939.t1 | AT3G52780 | 38.565 | 446 | 233 | 12 | 16 | 454 | 5 | 416 | 4.34e-85 | 268 |
| MS.gene74939.t1 | AT3G07130 | 33.929 | 448 | 206 | 12 | 77 | 450 | 80 | 511 | 3.63e-66 | 222 |
| MS.gene74939.t1 | AT2G32770 | 34.901 | 404 | 181 | 9 | 113 | 450 | 143 | 530 | 1.05e-65 | 221 |
| MS.gene74939.t1 | AT4G13700 | 36.438 | 365 | 174 | 11 | 77 | 399 | 80 | 428 | 6.23e-61 | 206 |
| MS.gene74939.t1 | AT3G07130 | 31.601 | 481 | 206 | 13 | 77 | 450 | 80 | 544 | 2.20e-59 | 204 |
| MS.gene74939.t1 | AT2G32770 | 33.508 | 382 | 172 | 9 | 113 | 428 | 62 | 427 | 3.20e-57 | 195 |
| MS.gene74939.t1 | AT3G52780 | 36.096 | 374 | 207 | 12 | 16 | 380 | 5 | 355 | 1.54e-56 | 192 |
| MS.gene74939.t1 | AT2G32770 | 31.683 | 404 | 165 | 10 | 113 | 450 | 143 | 501 | 5.72e-52 | 184 |
| MS.gene74939.t1 | AT4G13700 | 34.969 | 326 | 165 | 10 | 77 | 360 | 80 | 400 | 1.86e-49 | 174 |
| MS.gene74939.t1 | AT1G13900 | 25.541 | 462 | 256 | 18 | 47 | 444 | 132 | 569 | 1.31e-23 | 104 |
| MS.gene74939.t1 | AT5G50400 | 25.562 | 356 | 224 | 14 | 119 | 444 | 237 | 581 | 3.97e-20 | 94.0 |
| MS.gene74939.t1 | AT5G50400 | 25.562 | 356 | 224 | 14 | 119 | 444 | 165 | 509 | 4.20e-20 | 93.6 |
| MS.gene74939.t1 | AT2G03450 | 25.111 | 450 | 253 | 16 | 58 | 444 | 141 | 569 | 9.62e-20 | 92.8 |
| MS.gene74939.t1 | AT1G13750 | 25.549 | 364 | 214 | 14 | 119 | 444 | 239 | 583 | 1.32e-17 | 85.9 |
| MS.gene74939.t1 | AT4G24890 | 25.000 | 356 | 226 | 14 | 119 | 444 | 241 | 585 | 2.10e-17 | 85.5 |
Find 110 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGGTTTCGCCAATTTC+TGG | 0.125859 | 1.3:+76400226 | None:intergenic |
| TCACACTACTATAAGGAATT+TGG | 0.199886 | 1.3:-76400584 | MS.gene74939:CDS |
| CACCTAACATTGTCATGATT+TGG | 0.223594 | 1.3:+76400334 | None:intergenic |
| ATTGATTTCGCCCCAGAAAT+TGG | 0.247783 | 1.3:-76400238 | MS.gene74939:CDS |
| GTACTTTGTGATGCTTTATA+AGG | 0.272518 | 1.3:+76400172 | None:intergenic |
| GGCACATCAGGACCAATTTC+AGG | 0.281679 | 1.3:+76400490 | None:intergenic |
| TTTATAACTCCTCCTGAAAT+TGG | 0.285563 | 1.3:-76400502 | MS.gene74939:CDS |
| CACACGTTCGGTTCTTTCAT+AGG | 0.290239 | 1.3:+76399871 | None:intergenic |
| TAGCAACAATCAACATTATA+TGG | 0.299454 | 1.3:-76399975 | MS.gene74939:CDS |
| CACAAAGTACTTCACCCTTT+TGG | 0.304317 | 1.3:-76400159 | MS.gene74939:CDS |
| TTATTCATCACACTACTATA+AGG | 0.305033 | 1.3:-76400591 | MS.gene74939:CDS |
| GTTTCTGCCCTGTTAACTTT+AGG | 0.307508 | 1.3:+76400037 | None:intergenic |
| GTGATGCTTTATAAGGAGTA+TGG | 0.325433 | 1.3:+76400179 | None:intergenic |
| TGGCTTATCAACCATGGATA+TGG | 0.334997 | 1.3:-76400279 | MS.gene74939:CDS |
| CTGGGGCGAAATCAATTTCA+TGG | 0.339164 | 1.3:+76400245 | None:intergenic |
| AGGAGCTACCTAAAGTTAAC+AGG | 0.344003 | 1.3:-76400045 | MS.gene74939:CDS |
| GGAACACAACAAGACAGTTT+TGG | 0.351168 | 1.3:-76400525 | MS.gene74939:CDS |
| AGATTCTTCAATTACTCATC+TGG | 0.355881 | 1.3:-76400616 | MS.gene74939:CDS |
| GTTTGGTTTCGCCAATTTCT+GGG | 0.356914 | 1.3:+76400227 | None:intergenic |
| TACTATTATGAGGTTGGACT+TGG | 0.361764 | 1.3:-76400547 | MS.gene74939:CDS |
| ACCTAACATTGTCATGATTT+GGG | 0.368249 | 1.3:+76400335 | None:intergenic |
| GGTCGAACGCAGTGCGCTAC+TGG | 0.369778 | 1.3:-76400693 | MS.gene74939:CDS |
| ACAATGTTAGGTGGGATACT+TGG | 0.374862 | 1.3:-76400324 | MS.gene74939:CDS |
| CGAATAAGGTTTGAATGGTT+TGG | 0.378529 | 1.3:+76400210 | None:intergenic |
| GGGAAAACAGTGTTGTTTGT+TGG | 0.380568 | 1.3:-76400382 | MS.gene74939:CDS |
| AATTTGGTGTTTGTGTGTAA+TGG | 0.386262 | 1.3:-76400889 | MS.gene74939:CDS |
| GTATGGTATCGGTGCGAATA+AGG | 0.387101 | 1.3:+76400196 | None:intergenic |
| TAGAAATCAAGATGGTTATT+CGG | 0.395276 | 1.3:-76399633 | MS.gene74939:CDS |
| TGCAATATTCGACACACGTT+CGG | 0.401173 | 1.3:+76399859 | None:intergenic |
| TTTGGTCTCATAGGAGATCT+TGG | 0.404619 | 1.3:-76400460 | MS.gene74939:CDS |
| CCAAATGTGTATGGCACATC+AGG | 0.405903 | 1.3:+76400478 | None:intergenic |
| GCATTCAGAGAGGCTAGCTT+TGG | 0.414506 | 1.3:-76399707 | MS.gene74939:CDS |
| TGGTTATAATGCTCCCCAAC+AGG | 0.417531 | 1.3:-76400785 | MS.gene74939:CDS |
| CCATACACATTTGGTCTCAT+AGG | 0.418182 | 1.3:-76400469 | MS.gene74939:CDS |
| TCCCAAATCATGACAATGTT+AGG | 0.419243 | 1.3:-76400336 | MS.gene74939:CDS |
| TTCAGCTCACATCATTGTCT+TGG | 0.421163 | 1.3:-76400119 | MS.gene74939:CDS |
| CCTGATGTGCCATACACATT+TGG | 0.422920 | 1.3:-76400478 | MS.gene74939:CDS |
| CGGTGCGAATAAGGTTTGAA+TGG | 0.426529 | 1.3:+76400205 | None:intergenic |
| TCTCCAATGGTTATGTATAC+AGG | 0.430699 | 1.3:+76399788 | None:intergenic |
| TTACACAAGGTGATCTTGTT+GGG | 0.430973 | 1.3:-76400756 | MS.gene74939:CDS |
| TGAGCAGTGGAATCATCAAC+TGG | 0.435821 | 1.3:+76399569 | None:intergenic |
| TTGGCCTCGTATTCAGCATA+TGG | 0.441264 | 1.3:-76400100 | MS.gene74939:CDS |
| CAATGTTAGGTGGGATACTT+GGG | 0.445086 | 1.3:-76400323 | MS.gene74939:CDS |
| ATTACACAAGGTGATCTTGT+TGG | 0.449022 | 1.3:-76400757 | MS.gene74939:CDS |
| TGGCTGTGGTTCTGTCATGT+TGG | 0.470647 | 1.3:+76399739 | None:intergenic |
| CGGTTGAGGCTGATTCCCAT+TGG | 0.478638 | 1.3:-76399613 | MS.gene74939:CDS |
| CTTGGGGAAGGTTTGTAGAA+AGG | 0.482123 | 1.3:-76400306 | MS.gene74939:CDS |
| ACAGTACCAATGGCTTGAAC+AGG | 0.482376 | 1.3:-76400065 | MS.gene74939:CDS |
| ACTAAATACTATTATGAGGT+TGG | 0.489189 | 1.3:-76400553 | MS.gene74939:CDS |
| TTAACAGGGCAGAAACTCCT+TGG | 0.493051 | 1.3:-76400030 | MS.gene74939:CDS |
| GTAGCTCCTGTTCAAGCCAT+TGG | 0.495291 | 1.3:+76400059 | None:intergenic |
| TTGTGTAATATGAACCTGTT+GGG | 0.497585 | 1.3:+76400771 | None:intergenic |
| CCTATGAGACCAAATGTGTA+TGG | 0.500434 | 1.3:+76400469 | None:intergenic |
| AAGGTTGATGTTGTGTATGC+TGG | 0.513119 | 1.3:-76399905 | MS.gene74939:CDS |
| TCAAGATGGTTATTCGGTTG+AGG | 0.514507 | 1.3:-76399627 | MS.gene74939:CDS |
| GCCATGGTTTGTTAAGTACA+AGG | 0.515158 | 1.3:-76399924 | MS.gene74939:CDS |
| GGAGATGGAGGGAACCTTGG+AGG | 0.515637 | 1.3:-76399770 | MS.gene74939:CDS |
| ATTGGAGATGGAGGGAACCT+TGG | 0.515906 | 1.3:-76399773 | MS.gene74939:CDS |
| TAGCGCACTGCGTTCGACCC+TGG | 0.519503 | 1.3:+76400697 | None:intergenic |
| AAACAAATGGGTATTTCTCA+AGG | 0.519830 | 1.3:-76400955 | None:intergenic |
| GAACACATGCTCACTATAGC+TGG | 0.521332 | 1.3:-76399658 | MS.gene74939:CDS |
| TGGGTGACCGAGGATGAACC+AGG | 0.523413 | 1.3:-76400715 | MS.gene74939:CDS |
| AACAATCAACATTATATGGA+AGG | 0.525860 | 1.3:-76399971 | MS.gene74939:CDS |
| TGTTGGTTTCTGAGCCTCCA+AGG | 0.536845 | 1.3:+76399756 | None:intergenic |
| GTTCGACCCTGGTTCATCCT+CGG | 0.543679 | 1.3:+76400708 | None:intergenic |
| ATTGCATATAATGTTGTGAA+TGG | 0.545809 | 1.3:-76399842 | MS.gene74939:CDS |
| TACATAACCATTGGAGATGG+AGG | 0.550025 | 1.3:-76399782 | MS.gene74939:CDS |
| CAACACTAAATACTATTATG+AGG | 0.552300 | 1.3:-76400557 | MS.gene74939:CDS |
| GCTCCTGTATACATAACCAT+TGG | 0.553714 | 1.3:-76399791 | MS.gene74939:CDS |
| ACAATCAACATTATATGGAA+GGG | 0.554284 | 1.3:-76399970 | MS.gene74939:CDS |
| TCTCTGAATGCTGAGTACTC+TGG | 0.561355 | 1.3:+76399719 | None:intergenic |
| AATGCTGAGTACTCTGGCTG+TGG | 0.569296 | 1.3:+76399725 | None:intergenic |
| CTTTATAAGGAGTATGGTAT+CGG | 0.573117 | 1.3:+76400185 | None:intergenic |
| TTTCCTACAGTCCATATCCA+TGG | 0.573550 | 1.3:+76400268 | None:intergenic |
| CAAATCATGACAATGTTAGG+TGG | 0.575920 | 1.3:-76400333 | MS.gene74939:CDS |
| CAATCAACATTATATGGAAG+GGG | 0.577345 | 1.3:-76399969 | MS.gene74939:CDS |
| CAACCATGGATATGGACTGT+AGG | 0.580717 | 1.3:-76400271 | MS.gene74939:CDS |
| AATATACACCACAGTACCAA+TGG | 0.587307 | 1.3:-76400075 | MS.gene74939:CDS |
| TGTTCAAGCCATTGGTACTG+TGG | 0.587686 | 1.3:+76400067 | None:intergenic |
| GTATACATAACCATTGGAGA+TGG | 0.590351 | 1.3:-76399785 | MS.gene74939:CDS |
| TTGGTGTTTGTGTGTAATGG+AGG | 0.590798 | 1.3:-76400886 | MS.gene74939:CDS |
| GATCGTATCGTGGGTGACCG+AGG | 0.591550 | 1.3:-76400725 | MS.gene74939:CDS |
| TTAGGTGGGATACTTGGGGA+AGG | 0.593341 | 1.3:-76400318 | MS.gene74939:CDS |
| AAGGTTCCCTCCATCTCCAA+TGG | 0.594309 | 1.3:+76399775 | None:intergenic |
| AAATCATGACAATGTTAGGT+GGG | 0.598863 | 1.3:-76400332 | MS.gene74939:CDS |
| GGAAAGCAGTGATCGTATCG+TGG | 0.602783 | 1.3:-76400735 | MS.gene74939:CDS |
| ACTATTATGAGGTTGGACTT+GGG | 0.609032 | 1.3:-76400546 | MS.gene74939:CDS |
| TTTGGTTTCGCCAATTTCTG+GGG | 0.609945 | 1.3:+76400228 | None:intergenic |
| TTTGTTAGGAATGTTCAGAA+GGG | 0.615218 | 1.3:-76400850 | MS.gene74939:CDS |
| ACCTTGTACTTAACAAACCA+TGG | 0.616531 | 1.3:+76399923 | None:intergenic |
| TGAGAGTAATGTATGAGCCA+TGG | 0.618209 | 1.3:-76399940 | MS.gene74939:CDS |
| GAAAGCAGTGATCGTATCGT+GGG | 0.619977 | 1.3:-76400734 | MS.gene74939:CDS |
| AGCTGGCATAGAAATCAAGA+TGG | 0.628581 | 1.3:-76399641 | MS.gene74939:CDS |
| TCAATGTAAAACATGAGCAG+TGG | 0.648716 | 1.3:+76399556 | None:intergenic |
| TGCATTAGAACAATCAACCA+AGG | 0.650420 | 1.3:+76400013 | None:intergenic |
| CTTGTGTAATATGAACCTGT+TGG | 0.651645 | 1.3:+76400770 | None:intergenic |
| ACATAACCATTGGAGATGGA+GGG | 0.652593 | 1.3:-76399781 | MS.gene74939:CDS |
| GGAGCGTGGCTTATCAACCA+TGG | 0.653583 | 1.3:-76400285 | MS.gene74939:CDS |
| TGGGGAGCATTATAACCAGA+AGG | 0.658702 | 1.3:+76400790 | None:intergenic |
| CAACAGGTTCATATTACACA+AGG | 0.659532 | 1.3:-76400769 | MS.gene74939:CDS |
| TTGTTCTAATGCATTCACCA+TGG | 0.660660 | 1.3:-76400003 | MS.gene74939:CDS |
| AAGGTTTGTAGAAAGGAGCG+TGG | 0.671855 | 1.3:-76400299 | MS.gene74939:CDS |
| ACATCAGGACCAATTTCAGG+AGG | 0.690189 | 1.3:+76400493 | None:intergenic |
| GAGCAGTGGAATCATCAACT+GGG | 0.690782 | 1.3:+76399570 | None:intergenic |
| GGAGCTACCTAAAGTTAACA+GGG | 0.695753 | 1.3:-76400044 | MS.gene74939:CDS |
| TGATTGTTGCTATTATACCA+TGG | 0.695794 | 1.3:+76399986 | None:intergenic |
| GGGTGACCGAGGATGAACCA+GGG | 0.713113 | 1.3:-76400714 | MS.gene74939:CDS |
| AGAGTACTCAGCATTCAGAG+AGG | 0.715627 | 1.3:-76399717 | MS.gene74939:CDS |
| AATGTTAGGTGGGATACTTG+GGG | 0.732363 | 1.3:-76400322 | MS.gene74939:CDS |
| TGTGTAATATGAACCTGTTG+GGG | 0.733638 | 1.3:+76400772 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATCTGTTGAAAAACCAA+TGG | + | chr1.3:76400911-76400930 | None:intergenic | 25.0% |
| ! | AAACATAAAAAGCTAGCTAA+AGG | - | chr1.3:76399850-76399869 | MS.gene74939:CDS | 25.0% |
| ! | AAATCTGTTGAAAAACCAAT+GGG | + | chr1.3:76400910-76400929 | None:intergenic | 25.0% |
| ! | AACAATCAACATTATATGGA+AGG | - | chr1.3:76400534-76400553 | MS.gene74939:CDS | 25.0% |
| ! | AACATAAAAAGCTAGCTAAA+GGG | - | chr1.3:76399851-76399870 | MS.gene74939:CDS | 25.0% |
| ! | ACAATCAACATTATATGGAA+GGG | - | chr1.3:76400535-76400554 | MS.gene74939:CDS | 25.0% |
| ! | ACTAAATACTATTATGAGGT+TGG | - | chr1.3:76399952-76399971 | MS.gene74939:CDS | 25.0% |
| ! | ATGAATTGAATCCCAAAAAA+GGG | - | chr1.3:76400103-76400122 | MS.gene74939:CDS | 25.0% |
| ! | ATTGCATATAATGTTGTGAA+TGG | - | chr1.3:76400663-76400682 | MS.gene74939:CDS | 25.0% |
| ! | CAACACTAAATACTATTATG+AGG | - | chr1.3:76399948-76399967 | MS.gene74939:CDS | 25.0% |
| ! | TAGAAATCAAGATGGTTATT+CGG | - | chr1.3:76400872-76400891 | MS.gene74939:CDS | 25.0% |
| ! | TAGCAACAATCAACATTATA+TGG | - | chr1.3:76400530-76400549 | MS.gene74939:CDS | 25.0% |
| ! | TATGAATTGAATCCCAAAAA+AGG | - | chr1.3:76400102-76400121 | MS.gene74939:CDS | 25.0% |
| ! | TCTGTTCATTATGTCAAAAA+TGG | + | chr1.3:76400829-76400848 | None:intergenic | 25.0% |
| ! | TTATTCATCACACTACTATA+AGG | - | chr1.3:76399914-76399933 | MS.gene74939:CDS | 25.0% |
| !! | CTTTTGATAGAATACCAAAA+GGG | + | chr1.3:76400363-76400382 | None:intergenic | 25.0% |
| !! | TCTTTTGATAGAATACCAAA+AGG | + | chr1.3:76400364-76400383 | None:intergenic | 25.0% |
| !!! | ATTGGTTTTTCAACAGATTT+TGG | - | chr1.3:76400910-76400929 | MS.gene74939:CDS | 25.0% |
| !!! | TTTAGCTAGCTTTTTATGTT+TGG | + | chr1.3:76399851-76399870 | None:intergenic | 25.0% |
| AAATCATGACAATGTTAGGT+GGG | - | chr1.3:76400173-76400192 | MS.gene74939:CDS | 30.0% | |
| ACCTAACATTGTCATGATTT+GGG | + | chr1.3:76400173-76400192 | None:intergenic | 30.0% | |
| AGATTCTTCAATTACTCATC+TGG | - | chr1.3:76399889-76399908 | MS.gene74939:CDS | 30.0% | |
| CAATCAACATTATATGGAAG+GGG | - | chr1.3:76400536-76400555 | MS.gene74939:CDS | 30.0% | |
| GTACTTTGTGATGCTTTATA+AGG | + | chr1.3:76400336-76400355 | None:intergenic | 30.0% | |
| TCACACTACTATAAGGAATT+TGG | - | chr1.3:76399921-76399940 | MS.gene74939:CDS | 30.0% | |
| TTGTGTAATATGAACCTGTT+GGG | + | chr1.3:76399737-76399756 | None:intergenic | 30.0% | |
| TTTATAACTCCTCCTGAAAT+TGG | - | chr1.3:76400003-76400022 | MS.gene74939:CDS | 30.0% | |
| TTTGTTAGGAATGTTCAGAA+GGG | - | chr1.3:76399655-76399674 | MS.gene74939:CDS | 30.0% | |
| ! | AACACTGTTTTCCCTTTTTT+GGG | + | chr1.3:76400117-76400136 | None:intergenic | 30.0% |
| ! | CTTTATAAGGAGTATGGTAT+CGG | + | chr1.3:76400323-76400342 | None:intergenic | 30.0% |
| ! | TGATTGTTGCTATTATACCA+TGG | + | chr1.3:76400522-76400541 | None:intergenic | 30.0% |
| ! | TTTTGTTAGGAATGTTCAGA+AGG | - | chr1.3:76399654-76399673 | MS.gene74939:CDS | 30.0% |
| !! | AATTTGGTGTTTGTGTGTAA+TGG | - | chr1.3:76399616-76399635 | MS.gene74939:CDS | 30.0% |
| !!! | ACTAGCTCTAGTTTTGAATT+TGG | - | chr1.3:76399600-76399619 | MS.gene74939:CDS | 30.0% |
| AATATACACCACAGTACCAA+TGG | - | chr1.3:76400430-76400449 | MS.gene74939:CDS | 35.0% | |
| ACCTTGTACTTAACAAACCA+TGG | + | chr1.3:76400585-76400604 | None:intergenic | 35.0% | |
| ACTATTATGAGGTTGGACTT+GGG | - | chr1.3:76399959-76399978 | MS.gene74939:CDS | 35.0% | |
| ATTACACAAGGTGATCTTGT+TGG | - | chr1.3:76399748-76399767 | MS.gene74939:CDS | 35.0% | |
| CAAATCATGACAATGTTAGG+TGG | - | chr1.3:76400172-76400191 | MS.gene74939:CDS | 35.0% | |
| CAACAGGTTCATATTACACA+AGG | - | chr1.3:76399736-76399755 | MS.gene74939:CDS | 35.0% | |
| CACCTAACATTGTCATGATT+TGG | + | chr1.3:76400174-76400193 | None:intergenic | 35.0% | |
| CGAATAAGGTTTGAATGGTT+TGG | + | chr1.3:76400298-76400317 | None:intergenic | 35.0% | |
| CTTGTGTAATATGAACCTGT+TGG | + | chr1.3:76399738-76399757 | None:intergenic | 35.0% | |
| GTATACATAACCATTGGAGA+TGG | - | chr1.3:76400720-76400739 | MS.gene74939:CDS | 35.0% | |
| GTGATGCTTTATAAGGAGTA+TGG | + | chr1.3:76400329-76400348 | None:intergenic | 35.0% | |
| TACTATTATGAGGTTGGACT+TGG | - | chr1.3:76399958-76399977 | MS.gene74939:CDS | 35.0% | |
| TCAATGTAAAACATGAGCAG+TGG | + | chr1.3:76400952-76400971 | None:intergenic | 35.0% | |
| TCCCAAATCATGACAATGTT+AGG | - | chr1.3:76400169-76400188 | MS.gene74939:CDS | 35.0% | |
| TCTCCAATGGTTATGTATAC+AGG | + | chr1.3:76400720-76400739 | None:intergenic | 35.0% | |
| TGCATTAGAACAATCAACCA+AGG | + | chr1.3:76400495-76400514 | None:intergenic | 35.0% | |
| TGTGTAATATGAACCTGTTG+GGG | + | chr1.3:76399736-76399755 | None:intergenic | 35.0% | |
| TTACACAAGGTGATCTTGTT+GGG | - | chr1.3:76399749-76399768 | MS.gene74939:CDS | 35.0% | |
| TTGTTCTAATGCATTCACCA+TGG | - | chr1.3:76400502-76400521 | MS.gene74939:CDS | 35.0% | |
| ! | CAACACTGTTTTCCCTTTTT+TGG | + | chr1.3:76400118-76400137 | None:intergenic | 35.0% |
| ! | GGAGCTGATTGATCTTTTAT+AGG | + | chr1.3:76400699-76400718 | None:intergenic | 35.0% |
| ! | TTTTCCATATGCTGAATACG+AGG | + | chr1.3:76400412-76400431 | None:intergenic | 35.0% |
| AATGTTAGGTGGGATACTTG+GGG | - | chr1.3:76400183-76400202 | MS.gene74939:CDS | 40.0% | |
| ACAATGTTAGGTGGGATACT+TGG | - | chr1.3:76400181-76400200 | MS.gene74939:CDS | 40.0% | |
| ACATAACCATTGGAGATGGA+GGG | - | chr1.3:76400724-76400743 | MS.gene74939:CDS | 40.0% | |
| AGCTGGCATAGAAATCAAGA+TGG | - | chr1.3:76400864-76400883 | MS.gene74939:CDS | 40.0% | |
| AGGAGCTACCTAAAGTTAAC+AGG | - | chr1.3:76400460-76400479 | MS.gene74939:CDS | 40.0% | |
| CAATGTTAGGTGGGATACTT+GGG | - | chr1.3:76400182-76400201 | MS.gene74939:CDS | 40.0% | |
| CCATACACATTTGGTCTCAT+AGG | - | chr1.3:76400036-76400055 | MS.gene74939:CDS | 40.0% | |
| CCTATGAGACCAAATGTGTA+TGG | + | chr1.3:76400039-76400058 | None:intergenic | 40.0% | |
| GCTCCTGTATACATAACCAT+TGG | - | chr1.3:76400714-76400733 | MS.gene74939:CDS | 40.0% | |
| GGAGCTACCTAAAGTTAACA+GGG | - | chr1.3:76400461-76400480 | MS.gene74939:CDS | 40.0% | |
| GTTTCTGCCCTGTTAACTTT+AGG | + | chr1.3:76400471-76400490 | None:intergenic | 40.0% | |
| TACATAACCATTGGAGATGG+AGG | - | chr1.3:76400723-76400742 | MS.gene74939:CDS | 40.0% | |
| TCAAGATGGTTATTCGGTTG+AGG | - | chr1.3:76400878-76400897 | MS.gene74939:CDS | 40.0% | |
| TGAGAGTAATGTATGAGCCA+TGG | - | chr1.3:76400565-76400584 | MS.gene74939:CDS | 40.0% | |
| TGCAATATTCGACACACGTT+CGG | + | chr1.3:76400649-76400668 | None:intergenic | 40.0% | |
| TGGCTTATCAACCATGGATA+TGG | - | chr1.3:76400226-76400245 | MS.gene74939:CDS | 40.0% | |
| TTCAGCTCACATCATTGTCT+TGG | - | chr1.3:76400386-76400405 | MS.gene74939:CDS | 40.0% | |
| TTTCCTACAGTCCATATCCA+TGG | + | chr1.3:76400240-76400259 | None:intergenic | 40.0% | |
| TTTGGTCTCATAGGAGATCT+TGG | - | chr1.3:76400045-76400064 | MS.gene74939:CDS | 40.0% | |
| ! | ATTGATTTCGCCCCAGAAAT+TGG | - | chr1.3:76400267-76400286 | MS.gene74939:CDS | 40.0% |
| ! | GCACAACTAGTGCTTTTGTT+AGG | - | chr1.3:76399641-76399660 | MS.gene74939:CDS | 40.0% |
| ! | GGAACACAACAAGACAGTTT+TGG | - | chr1.3:76399980-76399999 | MS.gene74939:CDS | 40.0% |
| ! | GTTTGGTTTCGCCAATTTCT+GGG | + | chr1.3:76400281-76400300 | None:intergenic | 40.0% |
| ! | TTGGTGTTTGTGTGTAATGG+AGG | - | chr1.3:76399619-76399638 | MS.gene74939:CDS | 40.0% |
| ! | TTTGGTTTCGCCAATTTCTG+GGG | + | chr1.3:76400280-76400299 | None:intergenic | 40.0% |
| !! | AAGGTTGATGTTGTGTATGC+TGG | - | chr1.3:76400600-76400619 | MS.gene74939:CDS | 40.0% |
| !! | CACAAAGTACTTCACCCTTT+TGG | - | chr1.3:76400346-76400365 | MS.gene74939:CDS | 40.0% |
| !! | GCCATGGTTTGTTAAGTACA+AGG | - | chr1.3:76400581-76400600 | MS.gene74939:CDS | 40.0% |
| !! | GGGAAAACAGTGTTGTTTGT+TGG | - | chr1.3:76400123-76400142 | MS.gene74939:CDS | 40.0% |
| !!! | GATGTTTTTGCTGTTCCTTC+TGG | - | chr1.3:76399700-76399719 | MS.gene74939:CDS | 40.0% |
| AAGGTTTGTAGAAAGGAGCG+TGG | - | chr1.3:76400206-76400225 | MS.gene74939:CDS | 45.0% | |
| ACAGTACCAATGGCTTGAAC+AGG | - | chr1.3:76400440-76400459 | MS.gene74939:CDS | 45.0% | |
| ACATCAGGACCAATTTCAGG+AGG | + | chr1.3:76400015-76400034 | None:intergenic | 45.0% | |
| CACACGTTCGGTTCTTTCAT+AGG | + | chr1.3:76400637-76400656 | None:intergenic | 45.0% | |
| CCAAATGTGTATGGCACATC+AGG | + | chr1.3:76400030-76400049 | None:intergenic | 45.0% | |
| CCTGATGTGCCATACACATT+TGG | - | chr1.3:76400027-76400046 | MS.gene74939:CDS | 45.0% | |
| CGGTGCGAATAAGGTTTGAA+TGG | + | chr1.3:76400303-76400322 | None:intergenic | 45.0% | |
| CTGGGGCGAAATCAATTTCA+TGG | + | chr1.3:76400263-76400282 | None:intergenic | 45.0% | |
| CTTGGGGAAGGTTTGTAGAA+AGG | - | chr1.3:76400199-76400218 | MS.gene74939:CDS | 45.0% | |
| GAAAGCAGTGATCGTATCGT+GGG | - | chr1.3:76399771-76399790 | MS.gene74939:CDS | 45.0% | |
| GAACACATGCTCACTATAGC+TGG | - | chr1.3:76400847-76400866 | MS.gene74939:CDS | 45.0% | |
| GAGCAGTGGAATCATCAACT+GGG | + | chr1.3:76400938-76400957 | None:intergenic | 45.0% | |
| TCTCTGAATGCTGAGTACTC+TGG | + | chr1.3:76400789-76400808 | None:intergenic | 45.0% | |
| TGAGCAGTGGAATCATCAAC+TGG | + | chr1.3:76400939-76400958 | None:intergenic | 45.0% | |
| TGGTTATAATGCTCCCCAAC+AGG | - | chr1.3:76399720-76399739 | MS.gene74939:CDS | 45.0% | |
| TGTTCAAGCCATTGGTACTG+TGG | + | chr1.3:76400441-76400460 | None:intergenic | 45.0% | |
| TTAACAGGGCAGAAACTCCT+TGG | - | chr1.3:76400475-76400494 | MS.gene74939:CDS | 45.0% | |
| TTGGCCTCGTATTCAGCATA+TGG | - | chr1.3:76400405-76400424 | MS.gene74939:CDS | 45.0% | |
| ! | AGAGTACTCAGCATTCAGAG+AGG | - | chr1.3:76400788-76400807 | MS.gene74939:CDS | 45.0% |
| ! | CAACCATGGATATGGACTGT+AGG | - | chr1.3:76400234-76400253 | MS.gene74939:CDS | 45.0% |
| ! | GGTTTGGTTTCGCCAATTTC+TGG | + | chr1.3:76400282-76400301 | None:intergenic | 45.0% |
| !! | GTATGGTATCGGTGCGAATA+AGG | + | chr1.3:76400312-76400331 | None:intergenic | 45.0% |
| !! | TGGGGAGCATTATAACCAGA+AGG | + | chr1.3:76399718-76399737 | None:intergenic | 45.0% |
| AAGGTTCCCTCCATCTCCAA+TGG | + | chr1.3:76400733-76400752 | None:intergenic | 50.0% | |
| AATGCTGAGTACTCTGGCTG+TGG | + | chr1.3:76400783-76400802 | None:intergenic | 50.0% | |
| ATTGGAGATGGAGGGAACCT+TGG | - | chr1.3:76400732-76400751 | MS.gene74939:CDS | 50.0% | |
| GGAAAGCAGTGATCGTATCG+TGG | - | chr1.3:76399770-76399789 | MS.gene74939:CDS | 50.0% | |
| GGCACATCAGGACCAATTTC+AGG | + | chr1.3:76400018-76400037 | None:intergenic | 50.0% | |
| GTAGCTCCTGTTCAAGCCAT+TGG | + | chr1.3:76400449-76400468 | None:intergenic | 50.0% | |
| TTAGGTGGGATACTTGGGGA+AGG | - | chr1.3:76400187-76400206 | MS.gene74939:CDS | 50.0% | |
| ! | TGGCTGTGGTTCTGTCATGT+TGG | + | chr1.3:76400769-76400788 | None:intergenic | 50.0% |
| !! | GCATTCAGAGAGGCTAGCTT+TGG | - | chr1.3:76400798-76400817 | MS.gene74939:CDS | 50.0% |
| !! | TGTTGGTTTCTGAGCCTCCA+AGG | + | chr1.3:76400752-76400771 | None:intergenic | 50.0% |
| GGAGCGTGGCTTATCAACCA+TGG | - | chr1.3:76400220-76400239 | MS.gene74939:CDS | 55.0% | |
| GTTCGACCCTGGTTCATCCT+CGG | + | chr1.3:76399800-76399819 | None:intergenic | 55.0% | |
| ! | CGGTTGAGGCTGATTCCCAT+TGG | - | chr1.3:76400892-76400911 | MS.gene74939:CDS | 55.0% |
| GATCGTATCGTGGGTGACCG+AGG | - | chr1.3:76399780-76399799 | MS.gene74939:CDS | 60.0% | |
| GGAGATGGAGGGAACCTTGG+AGG | - | chr1.3:76400735-76400754 | MS.gene74939:CDS | 60.0% | |
| GGGTGACCGAGGATGAACCA+GGG | - | chr1.3:76399791-76399810 | MS.gene74939:CDS | 60.0% | |
| TGGGTGACCGAGGATGAACC+AGG | - | chr1.3:76399790-76399809 | MS.gene74939:CDS | 60.0% | |
| GGTCGAACGCAGTGCGCTAC+TGG | - | chr1.3:76399812-76399831 | MS.gene74939:CDS | 65.0% | |
| TAGCGCACTGCGTTCGACCC+TGG | + | chr1.3:76399811-76399830 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 76399556 | 76400971 | 76399556 | ID=MS.gene74939 |
| chr1.3 | mRNA | 76399556 | 76400971 | 76399556 | ID=MS.gene74939.t1;Parent=MS.gene74939 |
| chr1.3 | exon | 76399556 | 76400971 | 76399556 | ID=MS.gene74939.t1.exon1;Parent=MS.gene74939.t1 |
| chr1.3 | CDS | 76399556 | 76400971 | 76399556 | ID=cds.MS.gene74939.t1;Parent=MS.gene74939.t1 |
| Gene Sequence |
| Protein sequence |
