AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74958

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74958.t1 MTR_1g112310 96.429 644 13 2 1 635 1 643 0.0 1214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74958.t1 AT4G32640 59.104 670 195 22 1 635 1 626 0.0 642
MS.gene74958.t1 AT4G32640 59.104 670 195 22 1 635 1 626 0.0 642
MS.gene74958.t1 AT3G44340 73.743 358 70 4 278 635 296 629 8.21e-179 536
MS.gene74958.t1 AT3G44340 73.743 358 70 4 278 635 296 629 8.21e-179 536
MS.gene74958.t1 AT3G44340 73.743 358 70 4 278 635 296 629 9.46e-179 536
MS.gene74958.t1 AT3G44340 73.743 358 70 4 278 635 296 629 9.46e-179 536
MS.gene74958.t1 AT3G44340 73.743 358 70 4 278 635 296 629 9.46e-179 536
MS.gene74958.t1 AT3G07100 44.223 251 139 1 382 632 315 564 1.34e-70 247

Find 240 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAGGTCCTCCACGCTTTC+CGG 0.135319 1.3:+76168553 MS.gene74958:CDS
GCAACTCCCACCTTTGTATT+AGG 0.189796 1.3:-76173859 None:intergenic
AATTTGATTGGGATCAATTT+TGG 0.201366 1.3:-76169600 None:intergenic
TTTATGTAGGTTGTTGATTT+TGG 0.211927 1.3:+76171305 MS.gene74958:intron
TGATCAATAAATTTCATAAA+AGG 0.212445 1.3:-76171374 None:intergenic
ACGGGACTACCACTGCAATT+TGG 0.218028 1.3:+76172151 MS.gene74958:CDS
GGGCCTCCTCCGCAGAATTT+TGG 0.230269 1.3:+76168350 MS.gene74958:CDS
TCTCCACCTCTTCGTCCATC+TGG 0.258671 1.3:+76168329 MS.gene74958:CDS
CAGCCTCCTAGTTCACCTTT+TGG 0.269572 1.3:+76168710 MS.gene74958:CDS
TGACTCAGTGGCGCATTAAA+AGG 0.270372 1.3:-76168419 None:intergenic
CCTGGTCAACCTTCACCATT+TGG 0.278534 1.3:+76168230 MS.gene74958:CDS
TGACTCGGTGGCGCATTAAA+AGG 0.281625 1.3:-76168392 None:intergenic
CGGGACTACCACTGCAATTT+GGG 0.289360 1.3:+76172152 MS.gene74958:CDS
CAACTCCCACCTTTGTATTA+GGG 0.293126 1.3:-76173858 None:intergenic
CCAGGTCCTCCACGCTTTCC+GGG 0.304932 1.3:+76168554 MS.gene74958:CDS
ATTCAGAAGTTCTATTACTT+TGG 0.305920 1.3:-76174716 None:intergenic
GTTCCACCTCCTACAGTTCC+TGG 0.312588 1.3:+76169464 MS.gene74958:CDS
TCAGAATTAGGTCTGTAATT+AGG 0.319267 1.3:-76167960 None:intergenic
AGGTCATTGGAGGACGATTA+AGG 0.322208 1.3:-76168013 None:intergenic
GTCTGTAATAACTTGACTTA+TGG 0.328254 1.3:-76172770 None:intergenic
GAGGTCCCTGGAGTGGGCCT+TGG 0.335986 1.3:-76169623 None:intergenic
AATAATCATTGTTACTTGAA+TGG 0.339902 1.3:-76170581 None:intergenic
GATTACATTGTTAGAGATAC+TGG 0.340266 1.3:+76170002 MS.gene74958:CDS
TCACCTGCTGTTGGTCAAAC+TGG 0.342855 1.3:+76169557 MS.gene74958:CDS
GCACAGCCTCCCAGGATGTT+TGG 0.343036 1.3:+76169491 MS.gene74958:CDS
TGTTGCTACTAAAGAATTCA+TGG 0.343661 1.3:+76172238 MS.gene74958:CDS
GGTGGTCCAGTAGGCCTTCC+AGG 0.343961 1.3:-76168206 None:intergenic
GGTCCTCCTGGAAGGCCTAC+TGG 0.350494 1.3:+76168200 MS.gene74958:CDS
AGTGGGCCTTGGAATTTGAT+TGG 0.355778 1.3:-76169612 None:intergenic
GTGGGCCTTGGAATTTGATT+GGG 0.371049 1.3:-76169611 None:intergenic
ATGGCTGCTCCTGTGCCTCC+AGG 0.371669 1.3:+76167894 MS.gene74958:CDS
CTGGTCAACCTTCACCATTT+GGG 0.374508 1.3:+76168231 MS.gene74958:CDS
ATAATCATTGTTACTTGAAT+GGG 0.380090 1.3:-76170580 None:intergenic
TTGCAAACTGATGTAATTGT+TGG 0.380991 1.3:+76174517 MS.gene74958:CDS
AGGTGGACCGGGACGTGAAA+AGG 0.387956 1.3:-76168120 None:intergenic
GTTGTAGTCATAGATTGATT+TGG 0.389248 1.3:-76169530 None:intergenic
CTCGTCGGCGGGGCATTAAA+AGG 0.389714 1.3:-76168446 None:intergenic
TCAGCTAATCCATCAGAATT+AGG 0.393203 1.3:-76167972 None:intergenic
AGAACTTCTGAATCGGCCTT+TGG 0.395304 1.3:+76174726 MS.gene74958:CDS
CCCGTTACTCAACAAGCTAC+TGG 0.399181 1.3:-76168504 None:intergenic
CATGAAGTGCACAGTCAATC+AGG 0.400345 1.3:+76170043 MS.gene74958:CDS
AAGGTGGTTGTTGGGCAAAA+GGG 0.407872 1.3:-76168058 None:intergenic
CTTCCAGGAGGACCAGCATT+TGG 0.408386 1.3:-76168191 None:intergenic
TGCATTGGTGAGGCCAGGTG+GGG 0.413684 1.3:+76168148 MS.gene74958:CDS
CGGGACGTGAAAAGGGCGGC+TGG 0.413743 1.3:-76168112 None:intergenic
CCACCAAATGCTGGTCCTCC+TGG 0.414676 1.3:+76168188 MS.gene74958:CDS
CTGGTTCTGCACAGCCTCCC+AGG 0.416814 1.3:+76169483 MS.gene74958:CDS
GTGCATTGGTGAGGCCAGGT+GGG 0.421125 1.3:+76168147 MS.gene74958:CDS
GGCTATGTTAGTCCAACCTT+TGG 0.421615 1.3:+76170534 MS.gene74958:CDS
AAAGGTGGTTGTTGGGCAAA+AGG 0.424211 1.3:-76168059 None:intergenic
GCATCTCTACGTCGACCATC+TGG 0.429396 1.3:-76172176 None:intergenic
CTAGGTGCCCCTGGAGGCAC+AGG 0.430087 1.3:-76167903 None:intergenic
GTTTAACATGGCACCACCTC+AGG 0.431441 1.3:+76168679 MS.gene74958:CDS
GCAAAGGAGTATAAACATCT+TGG 0.431885 1.3:-76174498 None:intergenic
TGGCTGCTCCTGTGCCTCCA+GGG 0.432099 1.3:+76167895 MS.gene74958:CDS
ACACCAACACCGCCAGAAAC+CGG 0.432778 1.3:-76168290 None:intergenic
GCTAGAGGAGTTCTCATTGT+TGG 0.440023 1.3:-76168608 None:intergenic
ACTTGAATGGGCTCCTCAGA+TGG 0.440812 1.3:-76170568 None:intergenic
CCTGGCCTCACCAATGCACC+TGG 0.440859 1.3:-76168143 None:intergenic
AGGTCTAGCAACAGGATTAG+AGG 0.441911 1.3:-76168033 None:intergenic
CTCCACCTCTTCGTCCATCT+GGG 0.442047 1.3:+76168330 MS.gene74958:CDS
TCACGTCCCGGTCCACCTCC+AGG 0.442603 1.3:+76168125 MS.gene74958:CDS
CCAGTAGCTTGTTGAGTAAC+GGG 0.444773 1.3:+76168504 MS.gene74958:CDS
CAATGCACCTGGAGGTGGAC+CGG 0.449909 1.3:-76168132 None:intergenic
CCCGGAAAGCGTGGAGGACC+TGG 0.450552 1.3:-76168554 None:intergenic
GGTGGTTGACTACCGGGTCT+AGG 0.453570 1.3:-76167921 None:intergenic
ACACTGGTAGGTGGCATGCC+AGG 0.457095 1.3:-76168476 None:intergenic
TGAGGTGGTGCCATGTTAAA+CGG 0.458711 1.3:-76168677 None:intergenic
CTCCAGTGTCTTTCATTGCA+AGG 0.461418 1.3:-76175704 None:intergenic
GCATTGGTGAGGCCAGGTGG+GGG 0.462080 1.3:+76168149 MS.gene74958:CDS
GGTGCATTGGTGAGGCCAGG+TGG 0.465663 1.3:+76168146 MS.gene74958:CDS
AGGTGGAGGTGGTTGACTAC+CGG 0.465941 1.3:-76167928 None:intergenic
CCAGGTGCATTGGTGAGGCC+AGG 0.466413 1.3:+76168143 MS.gene74958:CDS
TGACGGAAAAGGTGGTTGTT+GGG 0.466785 1.3:-76168066 None:intergenic
TGCAAGATCACTGAGGTCCC+TGG 0.467202 1.3:-76169635 None:intergenic
GCACCAAAATTCTGCGGAGG+AGG 0.469948 1.3:-76168353 None:intergenic
GGTGGACCGGGACGTGAAAA+GGG 0.471279 1.3:-76168119 None:intergenic
CCTCCACGCTTTCCGGGTGG+TGG 0.472345 1.3:+76168560 MS.gene74958:CDS
CTGTAATTAGGTACATAGTT+AGG 0.472556 1.3:-76167948 None:intergenic
GCAACAGGATTAGAGGTCAT+TGG 0.472884 1.3:-76168026 None:intergenic
GGTGGTTGTTGGGCAAAAGG+GGG 0.473571 1.3:-76168056 None:intergenic
CAGGTGGAGCACTCGTCGGC+GGG 0.476324 1.3:-76168457 None:intergenic
ATGACGGAAAAGGTGGTTGT+TGG 0.479509 1.3:-76168067 None:intergenic
GCAGACTCAACAACAACAAC+AGG 0.480403 1.3:+76168745 MS.gene74958:CDS
TGGTGGCATTCCAAACATCC+TGG 0.480964 1.3:-76169501 None:intergenic
CCAAATGGTGAAGGTTGACC+AGG 0.482437 1.3:-76168230 None:intergenic
CCAGGAGGACCAGCATTTGG+TGG 0.482756 1.3:-76168188 None:intergenic
CTATTACTTTGGAACATGGT+AGG 0.483860 1.3:-76174705 None:intergenic
TCAATGAATGCTGTTCAAAC+TGG 0.486121 1.3:+76172724 MS.gene74958:CDS
AGGTGGTTGTTGGGCAAAAG+GGG 0.487855 1.3:-76168057 None:intergenic
GTATAAACATCTTGGACATC+AGG 0.488602 1.3:-76174490 None:intergenic
GGCTGGGAAGCATGGATCCC+TGG 0.490518 1.3:-76168095 None:intergenic
GGCCTTTGGTGCAGCAATCA+AGG 0.490870 1.3:+76174740 MS.gene74958:CDS
CAAATTCCAAGGCCCACTCC+AGG 0.490991 1.3:+76169617 MS.gene74958:CDS
AAGTAATAGAACTTCTGAAT+CGG 0.491268 1.3:+76174719 MS.gene74958:CDS
GGAAGCATGGATCCCTGGAC+CGG 0.494215 1.3:-76168090 None:intergenic
CTACCGGGTCTAGGTGCCCC+TGG 0.496601 1.3:-76167912 None:intergenic
GCAGGTGGTGCCATGTTAAA+CGG 0.496811 1.3:-76168647 None:intergenic
TTCTGCGGAGGAGGCCCAGA+TGG 0.498887 1.3:-76168344 None:intergenic
GGTACATAGTTAGGTGGAGG+TGG 0.500654 1.3:-76167939 None:intergenic
TGTCCGTTGCTCACGCTGCA+AGG 0.501269 1.3:+76171340 MS.gene74958:CDS
CACCTCCAGGTGCATTGGTG+AGG 0.502805 1.3:+76168138 MS.gene74958:CDS
GAAGTAATTCTAAATGTTGA+CGG 0.503315 1.3:-76174671 None:intergenic
CGGTCCACCTCCAGGTGCAT+TGG 0.504053 1.3:+76168133 MS.gene74958:CDS
ACCGTTCCTCTACATAGCTC+AGG 0.512395 1.3:-76172209 None:intergenic
TTTATGAAATTTATTGATCA+GGG 0.512817 1.3:+76171377 MS.gene74958:CDS
CGCTTTATCTGCAATTTGTG+TGG 0.513250 1.3:+76171404 MS.gene74958:CDS
GATTGATTTGGCAGTGGTGG+TGG 0.514930 1.3:-76169518 None:intergenic
CCATTTGGGTCAAGACCTAC+TGG 0.515597 1.3:+76168245 MS.gene74958:CDS
GCGCCGGTTTCTGGCGGTGT+TGG 0.517498 1.3:+76168287 MS.gene74958:CDS
CACTGCAATTTGGGTCCAGA+TGG 0.517740 1.3:+76172161 MS.gene74958:CDS
TCTGGAGGACCACCAAATGC+TGG 0.518709 1.3:+76168179 MS.gene74958:CDS
TGCCAAGAAGGTGCTCCAAA+AGG 0.519045 1.3:-76168725 None:intergenic
GGTGGAGGTGGTTGACTACC+GGG 0.520100 1.3:-76167927 None:intergenic
AATTACAGACCTAATTCTGA+TGG 0.520333 1.3:+76167963 MS.gene74958:CDS
TACCTTGATTGCTGCACCAA+AGG 0.520567 1.3:-76174742 None:intergenic
CCTCCAGGGGCACCTAGACC+CGG 0.520676 1.3:+76167909 MS.gene74958:CDS
AAAAGGGCGGCTGGGAAGCA+TGG 0.520850 1.3:-76168103 None:intergenic
CTTGAATGGGCTCCTCAGAT+GGG 0.521214 1.3:-76170567 None:intergenic
GGTGGAGAACCGCCAGGTGG+AGG 0.522755 1.3:-76168314 None:intergenic
ATCACTGAGGTCCCTGGAGT+GGG 0.524840 1.3:-76169629 None:intergenic
CTGGGAGGCTGTGCAGAACC+AGG 0.524988 1.3:-76169482 None:intergenic
GGCCCAGATGGACGAAGAGG+TGG 0.525765 1.3:-76168332 None:intergenic
CTCACCAATGCACCTGGAGG+TGG 0.529454 1.3:-76168137 None:intergenic
AGAGGAGTTCTCATTGTTGG+AGG 0.530055 1.3:-76168605 None:intergenic
CCCAGTAGCTTGTTGAGTAA+CGG 0.532425 1.3:+76168503 MS.gene74958:CDS
GACAACATCAGGAATGCAGT+TGG 0.532731 1.3:+76170513 MS.gene74958:CDS
CCTGGACCGGATGACGGAAA+AGG 0.533407 1.3:-76168077 None:intergenic
AAGAAGTACACAGCAGGCAT+TGG 0.533461 1.3:-76172688 None:intergenic
AATGCACCTGGAGGTGGACC+GGG 0.533935 1.3:-76168131 None:intergenic
GGGACGTGAAAAGGGCGGCT+GGG 0.534199 1.3:-76168111 None:intergenic
ATGTTAAACGGAGACTGAGC+AGG 0.535173 1.3:-76168665 None:intergenic
ACAATGTAATCACTTGTAGC+AGG 0.537673 1.3:-76169990 None:intergenic
TGTTGTTGAGTCTGCCAAGA+AGG 0.537851 1.3:-76168737 None:intergenic
GGCTGCTCCTGTGCCTCCAG+GGG 0.538674 1.3:+76167896 MS.gene74958:CDS
CCGTTACTCAACAAGCTACT+GGG 0.542267 1.3:-76168503 None:intergenic
CCAGTAGGTCTTGACCCAAA+TGG 0.543017 1.3:-76168245 None:intergenic
TCAAGACCTACTGGACCTCC+TGG 0.544414 1.3:+76168254 MS.gene74958:CDS
GGCTGCATTATTCCCTGAGG+TGG 0.544426 1.3:-76168692 None:intergenic
TTGCAGCGTGAGCAACGGAC+AGG 0.546046 1.3:-76171338 None:intergenic
AGGCCTACTGGACCACCTCC+TGG 0.546283 1.3:+76168212 MS.gene74958:CDS
CTTGAAACGTGCATTGCAGC+AGG 0.546914 1.3:+76173912 MS.gene74958:CDS
CCGCCGACGAGTGCTCCACC+TGG 0.556817 1.3:+76168458 MS.gene74958:CDS
GGTGCTCCAAAAGGTGAACT+AGG 0.557161 1.3:-76168716 None:intergenic
TTAGGTACATAGTTAGGTGG+AGG 0.559199 1.3:-76167942 None:intergenic
GTCAAAAGTTCAGCAGTGAA+AGG 0.559372 1.3:-76170493 None:intergenic
TGCTCAGTCTCCGTTTAACA+TGG 0.561513 1.3:+76168637 MS.gene74958:CDS
CCAGGTGGAGCACTCGTCGG+CGG 0.563190 1.3:-76168458 None:intergenic
GACTGAGCAGACGGAGCTAG+AGG 0.566221 1.3:-76168623 None:intergenic
GGCACTTCCACACCCATCTG+AGG 0.566976 1.3:+76170555 MS.gene74958:CDS
CTGGGTGGCACACTGGTAGG+TGG 0.569610 1.3:-76168485 None:intergenic
TCAGATGAATACCGGAGGGA+TGG 0.569846 1.3:-76169684 None:intergenic
CTACTGGGTGGCACACTGGT+AGG 0.571736 1.3:-76168488 None:intergenic
CGAAGAGGTGGAGAACCGCC+AGG 0.571919 1.3:-76168320 None:intergenic
GATCACTGAGGTCCCTGGAG+TGG 0.572603 1.3:-76169630 None:intergenic
TGGATCCCTGGACCGGATGA+CGG 0.572730 1.3:-76168083 None:intergenic
ATTAGAAAGAAGTACACAGC+AGG 0.576702 1.3:-76172694 None:intergenic
GGTGTTGGTGTTCCTCCACC+TGG 0.577637 1.3:+76168302 MS.gene74958:CDS
AAGCCTTGCAGCGTGAGCAA+CGG 0.580266 1.3:-76171343 None:intergenic
TTTGGAAGGCCCTGCCATGG+GGG 0.580861 1.3:-76169582 None:intergenic
GGTGAAGGTTGACCAGGAGG+TGG 0.581123 1.3:-76168224 None:intergenic
ACCGGCGCTGCAAAAGAACC+AGG 0.581999 1.3:-76168272 None:intergenic
GAACCAGGAACTGTAGGAGG+TGG 0.583747 1.3:-76169467 None:intergenic
CAAATGCTGGTCCTCCTGGA+AGG 0.584284 1.3:+76168192 MS.gene74958:CDS
CAAGCTACTGGGTGGCACAC+TGG 0.584337 1.3:-76168492 None:intergenic
TGACCAGGAGGTGGTCCAGT+AGG 0.584659 1.3:-76168215 None:intergenic
ACAATTACATCAGTTTGCAA+AGG 0.585966 1.3:-76174514 None:intergenic
CTCATTGTTGGAGGTCCAAG+TGG 0.586108 1.3:-76168596 None:intergenic
TTTGCAGCGCCGGTTTCTGG+CGG 0.588588 1.3:+76168281 MS.gene74958:CDS
GGTTGTTGCACACCACCACC+CGG 0.589180 1.3:-76168572 None:intergenic
GGTGGCATTCCAAACATCCT+GGG 0.590788 1.3:-76169500 None:intergenic
TGCTCAGTCTCCGTTTAACA+TGG 0.590858 1.3:+76168667 MS.gene74958:CDS
GTCATAGATTGATTTGGCAG+TGG 0.592405 1.3:-76169524 None:intergenic
GCATTTGGTGGTCCTCCAGA+AGG 0.593521 1.3:-76168176 None:intergenic
CAATCAAGCAACCATCCCTC+CGG 0.594537 1.3:+76169673 MS.gene74958:CDS
GGTGTGGAAGTGCCAAAGGT+TGG 0.596402 1.3:-76170546 None:intergenic
GCTCCAGTTTGACCAACAGC+AGG 0.597183 1.3:-76169560 None:intergenic
TTGATCCCAATCAAATTCCA+AGG 0.598588 1.3:+76169606 MS.gene74958:CDS
TGTGCAGAACCAGGAACTGT+AGG 0.602383 1.3:-76169473 None:intergenic
ACACAGCAGGCATTGGCTCA+CGG 0.603215 1.3:-76172681 None:intergenic
GCCTGAGCTATGTAGAGGAA+CGG 0.605938 1.3:+76172208 MS.gene74958:CDS
GAAGGCCCTAATACAAAGGT+GGG 0.606579 1.3:+76173853 MS.gene74958:CDS
GATTTAGTGACGAAACTCCA+CGG 0.608853 1.3:+76172132 MS.gene74958:CDS
ATTGTTGGAGGTCCAAGTGG+TGG 0.610630 1.3:-76168593 None:intergenic
ACAACTATATCACCTGCTGT+TGG 0.610946 1.3:+76169548 MS.gene74958:CDS
AAATTCCAAGGCCCACTCCA+GGG 0.611989 1.3:+76169618 MS.gene74958:CDS
AGTTCTATTACTTTGGAACA+TGG 0.612944 1.3:-76174709 None:intergenic
ATAGCCCCACCAGAAAATGC+TGG 0.613309 1.3:-76168530 None:intergenic
ACAGGAAGGCCCTAATACAA+AGG 0.614202 1.3:+76173849 MS.gene74958:intron
ATAGATTGATTTGGCAGTGG+TGG 0.615250 1.3:-76169521 None:intergenic
GGTGTGCAACAACCACCACT+TGG 0.616166 1.3:+76168581 MS.gene74958:CDS
ATGCCAGGTGGAGCACTCGT+CGG 0.617763 1.3:-76168461 None:intergenic
ATGGGCTCCTCAGATGGGTG+TGG 0.617918 1.3:-76170562 None:intergenic
CTGGTAGGTGGCATGCCAGG+TGG 0.618714 1.3:-76168473 None:intergenic
AAAGAACCAGGAGGTCCAGT+AGG 0.618898 1.3:-76168260 None:intergenic
GGAAGGCCCTAATACAAAGG+TGG 0.624953 1.3:+76173852 MS.gene74958:intron
GGACCGGGACGTGAAAAGGG+CGG 0.626581 1.3:-76168116 None:intergenic
TCAGGAACAATGAGCATCAG+TGG 0.626806 1.3:-76174472 None:intergenic
GATGGGTGTGGAAGTGCCAA+AGG 0.628527 1.3:-76170550 None:intergenic
TTTAACATGGCACCACCTCA+GGG 0.630599 1.3:+76168680 MS.gene74958:CDS
GGTCTTGACCCAAATGGTGA+AGG 0.632905 1.3:-76168239 None:intergenic
ACACCACCACCCGGAAAGCG+TGG 0.633187 1.3:-76168563 None:intergenic
TTAAAAGGACTTTGACTCGG+TGG 0.633689 1.3:-76168404 None:intergenic
TGTAATTGTTGGACTATCCG+AGG 0.634338 1.3:+76174528 MS.gene74958:CDS
GGACCGGATGACGGAAAAGG+TGG 0.634815 1.3:-76168074 None:intergenic
GTTGGTGTTCCTCCACCTGG+CGG 0.635260 1.3:+76168305 MS.gene74958:CDS
ATTTAGTGACGAAACTCCAC+GGG 0.635572 1.3:+76172133 MS.gene74958:CDS
GCATTAAAAGGACTTTGACT+CGG 0.637794 1.3:-76168407 None:intergenic
ATCTTGCACGAGACTCGTCA+AGG 0.638122 1.3:+76169650 MS.gene74958:CDS
TTTGGTGGTCCTCCAGAAGG+AGG 0.638167 1.3:-76168173 None:intergenic
GGAGGCCCAGATGGACGAAG+AGG 0.638167 1.3:-76168335 None:intergenic
GCAGAACCAGGAACTGTAGG+AGG 0.638391 1.3:-76169470 None:intergenic
ACAGGATTAGAGGTCATTGG+AGG 0.644638 1.3:-76168023 None:intergenic
GAGAAGCCTGAGCTATGTAG+AGG 0.646821 1.3:+76172203 MS.gene74958:CDS
TAATTAGGTACATAGTTAGG+TGG 0.647440 1.3:-76167945 None:intergenic
AATGGTGAAGGTTGACCAGG+AGG 0.648119 1.3:-76168227 None:intergenic
TTAAACGGAGACTGAGCAGA+CGG 0.654762 1.3:-76168632 None:intergenic
ACTTATGGCACTGCAAGCTG+CGG 0.655728 1.3:-76172755 None:intergenic
TTAAAAGGACTTTGACTCAG+TGG 0.655934 1.3:-76168431 None:intergenic
GGCCTCACCAATGCACCTGG+AGG 0.656875 1.3:-76168140 None:intergenic
AAATTGCAGTGGTAGTCCCG+TGG 0.658549 1.3:-76172149 None:intergenic
CGAGCACCAAAATTCTGCGG+AGG 0.666333 1.3:-76168356 None:intergenic
CCACCACCCGGAAAGCGTGG+AGG 0.668200 1.3:-76168560 None:intergenic
GCTCCAAAAGGTGAACTAGG+AGG 0.669307 1.3:-76168713 None:intergenic
GGTCCTCCACGCTTTCCGGG+TGG 0.671125 1.3:+76168557 MS.gene74958:CDS
AGGTGGAGCACTCGTCGGCG+GGG 0.677236 1.3:-76168456 None:intergenic
GCCCCACCAGAAAATGCTGG+TGG 0.682330 1.3:-76168527 None:intergenic
GCATTAAAAGGACTTAGACT+CGG 0.684746 1.3:-76168380 None:intergenic
GGCATTCCAAACATCCTGGG+AGG 0.685392 1.3:-76169497 None:intergenic
AGAGGTGGAGAACCGCCAGG+TGG 0.688436 1.3:-76168317 None:intergenic
GGACGAGCACCAAAATTCTG+CGG 0.691688 1.3:-76168359 None:intergenic
CATCTGGACCCAAATTGCAG+TGG 0.694884 1.3:-76172160 None:intergenic
TTAAACGGAGACTGAGCAGG+TGG 0.697518 1.3:-76168662 None:intergenic
CCGGGTCTAGGTGCCCCTGG+AGG 0.707354 1.3:-76167909 None:intergenic
ACTGTGCACTTCATGTAACG+TGG 0.707571 1.3:-76170035 None:intergenic
GGAGGCTGCATTATTCCCTG+AGG 0.723316 1.3:-76168695 None:intergenic
TTACTCAACAAGCTACTGGG+TGG 0.734418 1.3:-76168500 None:intergenic
GGCGCTGCAAAAGAACCAGG+AGG 0.734483 1.3:-76168269 None:intergenic
GGTCCAGTAGGCCTTCCAGG+AGG 0.772397 1.3:-76168203 None:intergenic
AGTCTCGTGCAAGATCACTG+AGG 0.789312 1.3:-76169642 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.3 gene 76167894 76175714 76167894 ID=MS.gene74958
chr1.3 mRNA 76167894 76175714 76167894 ID=MS.gene74958.t1;Parent=MS.gene74958
chr1.3 exon 76167894 76168766 76167894 ID=MS.gene74958.t1.exon1;Parent=MS.gene74958.t1
chr1.3 CDS 76167894 76168766 76167894 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76169461 76169694 76169461 ID=MS.gene74958.t1.exon2;Parent=MS.gene74958.t1
chr1.3 CDS 76169461 76169694 76169461 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76169990 76170064 76169990 ID=MS.gene74958.t1.exon3;Parent=MS.gene74958.t1
chr1.3 CDS 76169990 76170064 76169990 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76170490 76170588 76170490 ID=MS.gene74958.t1.exon4;Parent=MS.gene74958.t1
chr1.3 CDS 76170490 76170588 76170490 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76171314 76171425 76171314 ID=MS.gene74958.t1.exon5;Parent=MS.gene74958.t1
chr1.3 CDS 76171314 76171425 76171314 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76172132 76172259 76172132 ID=MS.gene74958.t1.exon6;Parent=MS.gene74958.t1
chr1.3 CDS 76172132 76172259 76172132 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76172679 76172798 76172679 ID=MS.gene74958.t1.exon7;Parent=MS.gene74958.t1
chr1.3 CDS 76172679 76172798 76172679 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76173853 76173933 76173853 ID=MS.gene74958.t1.exon8;Parent=MS.gene74958.t1
chr1.3 CDS 76173853 76173933 76173853 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76174472 76174549 76174472 ID=MS.gene74958.t1.exon9;Parent=MS.gene74958.t1
chr1.3 CDS 76174472 76174549 76174472 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76174669 76174761 76174669 ID=MS.gene74958.t1.exon10;Parent=MS.gene74958.t1
chr1.3 CDS 76174669 76174761 76174669 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
chr1.3 exon 76175697 76175714 76175697 ID=MS.gene74958.t1.exon11;Parent=MS.gene74958.t1
chr1.3 CDS 76175697 76175714 76175697 ID=cds.MS.gene74958.t1;Parent=MS.gene74958.t1
Gene Sequence

>MS.gene74958

ATGGCTGCTCCTGTGCCTCCAGGGGCACCTAGACCCGGTAGTCAACCACCTCCACCTAACTATGTACCTAATTACAGACCTAATTCTGATGGATTAGCTGATAATTTCAACAATTTGAACCTTAATCGTCCTCCAATGACCTCTAATCCTGTTGCTAGACCTCCCCCTTTTGCCCAACAACCACCTTTTCCGTCATCCGGTCCAGGGATCCATGCTTCCCAGCCGCCCTTTTCACGTCCCGGTCCACCTCCAGGTGCATTGGTGAGGCCAGGTGGGGGTCCTCCTTCTGGAGGACCACCAAATGCTGGTCCTCCTGGAAGGCCTACTGGACCACCTCCTGGTCAACCTTCACCATTTGGGTCAAGACCTACTGGACCTCCTGGTTCTTTTGCAGCGCCGGTTTCTGGCGGTGTTGGTGTTCCTCCACCTGGCGGTTCTCCACCTCTTCGTCCATCTGGGCCTCCTCCGCAGAATTTTGGTGCTCGTCCGAGTCTAAGTCCTTTTAATGCGCCACCGAGTCAAAGTCCTTTTAATGCGCCACTGAGTCAAAGTCCTTTTAATGCCCCGCCGACGAGTGCTCCACCTGGCATGCCACCTACCAGTGTGCCACCCAGTAGCTTGTTGAGTAACGGGCCACCAGCATTTTCTGGTGGGGCTATGCCAGGTCCTCCACGCTTTCCGGGTGGTGGTGTGCAACAACCACCACTTGGACCTCCAACAATGAGAACTCCTCTAGCTCCGTCTGCTCAGTCTCCGTTTAACATGGCACCACCTGCTCAGTCTCCGTTTAACATGGCACCACCTCAGGGAATAATGCAGCCTCCTAGTTCACCTTTTGGAGCACCTTCTTGGCAGACTCAACAACAACAACAGGTACCTACTCTGTTACATACTAGTATGCATGATTAGACTTTTGTCAGCATGTTTAAAAGAAAAAATATTTGGTGGCTGTAATATCTTAGCATAACTGGAGGTACGTTTGGTTAGACGAATGAAAGGTTGAGCTGTATTTGTTACTCTTATACGAAAAGCAGAAGATTAGTTTATGCATTTATATACAAGGGTGCCATGTGTACGTGTCAGCATTACTCAGTAAGGCTGTAGCCTTGTAGGTACTAAAGCAATGATGGTGCCTATTAATTTGACTGCAAGTGGCTACAGGCATAATACGGTATGTTGCCCTTGCGGCTGTGTGATGTGATGATGTAGCTTTTTAATTTATGCTTCAGGCTGAGGTTTGGTATAAAAAACAGTCTGTAAAATGTAAGCCTTAGCCTGAAAAATACGAGAAACTATGGTTAAGCTGTTATCTAAATTTTCAAAACTCATCTGTTCCCCGTCTACTGTGTATCACAGGTGCACCAATTAGCATATCATTACGTAATTAACAGACTATCATAGTGTGAGAGTTTGTGTGTTCTTAGCATGTAATACAAATAAATGGGTATTTGGCAACAGTAAAAATTCACATGCATAGTTGTGGGCATTAGTATTTATTTGTGTGTTGTACCCACATGCATTTATTCATCTTCTGAAAACCAGTCAATGAACATTTGTAATTCTGCAGTTAGTTCCACCTCCTACAGTTCCTGGTTCTGCACAGCCTCCCAGGATGTTTGGAATGCCACCACCACTGCCAAATCAATCTATGACTACAACTATATCACCTGCTGTTGGTCAAACTGGAGCCCCCATGGCAGGGCCTTCCAAAATTGATCCCAATCAAATTCCAAGGCCCACTCCAGGGACCTCAGTGATCTTGCACGAGACTCGTCAAGGCAATCAAGCAACCATCCCTCCGGTATTCATCTGAAACTTCTGGATAAATGATTTTCTCTTTTTAAATATAAAACAGATAGAGAATTGGCTTTATGTTGAAACTGAGGCTTGCTAAGCGTGTTTGCATATTCTTGGTTTTTGTATTATTTATTAGTTATTCATTTCCTTCTCTATTATTACATTTTTCCTTTTACATTATTTTATTTCAAATTTTTTTTTCAATATGTAGTTTCATAGCCGCTGCAATGTCTATTTTTGGGGAAAGTCCTATATACCACTACTGCTGTTTGATCCTATGATTTTTATTTTTTGTCCAGCCTGCTACAAGTGATTACATTGTTAGAGATACTGGCAATTGCAGTCCACGTTACATGAAGTGCACAGTCAATCAGGTAATGTTTAATATCTTCAAAATATTTTTAGCTATGAATAAGGTCATGAGAAATGCCAAGGTTTCTTGCCTGTGTCACTTATGTAGAGTTCTATTTGTGGCTTCTATGTTGTGGTTACTTAAAGGTTGCTTTTGTGGGTTGGTGATCACATATACAGAGGGGTTGAAGGGTTTTATTCTTTGATATATTTTTTATGCTTTGGATTCCGAAGAGGTTTTTTATTTTTATCATTTTTGCATAAGAAGACGCGTTATTCTCTTTCCCCGTATTTTTTTTCTTTCTAAAGATTAAATTCTGTTGTTCTAAGTGAATTTTGGTGTTAATTTTCTGAGATTAGTTATTTCAATATTGAAAATTTTGACATTTCTATCATTTCATATTAATTTTTTTTTTCATTGCTTTGTTCCTTTTGTTGATCGAAACAGATTCCTTTCACTGCTGAACTTTTGACAACATCAGGAATGCAGTTGGCTATGTTAGTCCAACCTTTGGCACTTCCACACCCATCTGAGGAGCCCATTCAAGTAACAATGATTATTCCTTTGTAGTTTTTGCATCTGTTTTTTATCCTTACAAAAATTAATTGATGTGTTAGCTGTAGAAGTTTCTATACAACAACCAAGCCTTACCCTACTAAGTGGGGTCGGCTACATGGATACTGTTCTATCACAAATCATAGCTGTAGAAGTTTTTATAGCCGTGTTTAATTTTTGTTGAAGTGCTATTGTTCGCTATTTTTTTGAAGTTATGGAGGATTCATTTAGTAGGGTGACTTACGTACATTTCACTTTCACGTGCTATGAACTTAATTATGCAAATAAAAAATTCAGATTTTTCTTTATCTGTCAAATGTTTGATGGCATGCTAGCTATTTGCAGCATTACTGCTTTTGGAAATCAAACATACATGCCATTTTAAGGATTCATGAAATGACATCTTTTGGGATTCTTTGTTTATGTTATAACTTGCTGTGGCTTTCTTATATTTGCTTGTATTTCCTCAGAGAATGCTAAAAATATACATCTTTCAAGTTTTGTTGTCTGTTTATGTTTGAACCAATAAGATTGGTGGTTGGTGTTGAGCCCATAAATACTGTATTCAAATAATTTATTGTATAGCTGTAGTAGTAGATATAAATTATTGTATGAAGTTTGTGAATTTCCCGCTCTGCTATTTCTAACATGTTTGTATAGGTTGTTGTAATAACTTCTAAATATATCTTTTGTCCAAATATCCAATGTTTATGTAGGTTGTTGATTTTGGAGAGAGTGGTCCTGTCCGTTGCTCACGCTGCAAGGCTTACGTTAATCCTTTTATGAAATTTATTGATCAGGGAAGACGCTTTATCTGCAATTTGTGTGGTAAGTATTATTACAATTGCACATCCTACTATTGTTTGTTGGTACTTGGTATATGCTATGGTAGGCTGTTTGGAGATGAATTTTACGTTAACATTTCCCTAGAATGAAGAGAGAAGAAATAGAAAGCTTTATTTTGTCATTTACTGGCAAAGTCAATCAATAGTTGTTGGTTGAGGACAATCAATCATATTACAAGTTTATGCGTATCTTGCAACTTATTCATTGCTCACTTTTAAATGTGATTATTGCTGCTAATTTTACTTTTTTTCTTTCTATTTTGATAAAAGAAATTCATTAAGACAGAAGTATGCACAAGAGATTGGAGGATAAGGGGTTTTCTTTTTGGTGTAAACGAAAAATAAAGCAACAAAGATAAATAGAAAATATTCATCAGATGGGTAGGTGTGACAGGATGACAGACATATTTCATTGCAGGGAGTCTTAACCAGTATCAATGTGATAATCAGAGGGCAAGGATAATTCCATAGAATTCTCTCTCCTCCTATCTCTGAGGCTATTTTTGTTGTGTGAAAAAAATTACTAGAGTGTGTGGAAGAGACAAAGGAGTGAAAGATTGAGTGGATTTGATTTATTGTTTGGTATACAAGAGAAAAAGGAGAGAAATATAAGAGGATGGGTCTATTCAATATTTTGCCTTTTGCGACATCGGTAACTATTTAACTGTGGATGTACATCTGTTTTACAGGATTTAGTGACGAAACTCCACGGGACTACCACTGCAATTTGGGTCCAGATGGTCGACGTAGAGATGCTGATGAGAAGCCTGAGCTATGTAGAGGAACGGTTGAGTTTGTTGCTACTAAAGAATTCATGGTTAGTTTATTTATAAAAAATACTTGTAGACTAAAATTATGAATTTATTAGTTAGCTCTTTACCAAGCCTAATAATTCTTGGGTAAATTTGTTTTCGTAATATACACTAAATCAAGTGTTGAGGCAATTTACCTTCAAGCCTATCCATCTTGATGTAATTGACGGCTGTTATAAATGGCTGTTTGTTCATAGAGTCATGTTTATTTGGTCTTTTTGTATACTGGATTGATAAAGTATAGTTAATTATACGATGGCCTAAATTTACGTTACGGTTCTAAGATGATATGCTAAGTAAGCAGTTTAGGCTTTTGTATGGAATACATAGTTTCCTTCTCTTTTCTTTGTTTTTTTTTTAATTTTATTTCTGTCATCCTTAAGTGTCCAATGATGAATAGTGTCCAATTCACTGTTTTGTGTAGGTCCGTGAGCCAATGCCTGCTGTGTACTTCTTTCTAATTGATGTATCAATGAATGCTGTTCAAACTGGTGCAACAGCCGCAGCTTGCAGTGCCATAAGTCAAGTTATTACAGACCTTCCTGTGAGTTCTAACTGTCACAACTTACCAATCATTTATTCATGATAATCTTTGTGAAGCTTAATGTTTTTCAATTTTTCTTTTGTTTGATTGTTTGAAACTTATTCCATTTATATTGGTGCTTTACTATCACAAGACAAAACCTTTAAGATTCTTCCTCCTCTCTGCTCCTGCATCCAAAACAAAAACAAAATAAACTGAACAAATTGTTTAAATTGAACAGGGTGATATGAAAAATCAGATTCCCACCAAGTTGGTCCCTTATAATAGCTTGACTATCATTTTTGTAATTTATTGACTATGCATTGTCTTGTGCGAGATTTTCGGTCTTTTGGTCCTATATTACGTTAATTTGTGAGGCACATCTTTGATTAAGATGTCACAAAACACCATCAATGCTAAGCTTGTGTGACCATTTACAAATCTGTTTAAAATTCTAGTCAGTTAAACTCCAAAGAGCACAATTGGCCATATTTTCCACAATGAATTATAACAACTTTCTTGCCTGCATTCAATGGATTGAGGATTCCAATGTTTGATATGAGTAGGACATGAGAACGGCGTGATTCAATATTTATAGGTACGATCACATTCATCATATGCTAAACATTTTCCACAATGTAGACTATAGATATTAAAATTAAATTCTTATTGTTAGGGCCAAACTCAGTGAAATAGTGGGGAGACTAGAAAAGGGAGAAGTGTATGCACATACATGAAATTACTCATTGGATGAAAACAGTAATGGCTTATAAGCCTGACCAATTACCCCAAAGGAAAACCAAGTTACCTGCTATCATATAATTTGGAGAGTCAAAACATTGAAACTTAAGTAATTTTTTTATATTAGACGGAATATTTATAGTCTGTTATTGTTATTTTTTCACTCCATTTGCCAGTCCTCTGTTTATTTTTTGTGGTTACTTATCTAACTCAATTTCTACCAGAACTCTTTTATTTATTTGAAATTTGAGTGGTGCGACCACAGATTCTGACAAATATCTTATTTTGTTTGACATTGAGTTCATGACTTGGTTTTTTCTTCTTATTACTACAGGAAGGCCCTAATACAAAGGTGGGAGTTGCAACGTTTGACTCGACAATTCATTTCTACAACTTGAAACGTGCATTGCAGCAGGTAGCACATAAGTACCCAACTAAACATACTGAGTTTACCAATTTTTTTTGTTTTTTTGTTTTTTTGTTATGGCATATTTACTGTTAAACCCATATTTTAATTCAGTTTAAATTAGTTGCAAAATTAAGGATATATAATATTTAGGTGAATTTGAAGGTGGAAAAATATTAGCTACAAGGGCCGAATATTGCCGCAGGCATTAGTTTCAATGTTTCTTCTTGTAGAGGGTGAATGAAATTTAGGATTTGTATACTATATATTTAATTGGTGTCCAGCAAACTGACCGGTCATATTGCCATTGTTTGAGTCTGTTTTTCCTGTCTCTCAGCAGTGAAAACAGCGGTTTTCTGTTATTATTTGTTTTCTTTGCTGTTTTTAACTTGAGACTGTATTGTGAAATTTAGAACACAATATTCCTTTGCGACTTGTCAAACACGGTGCCAATTAACTAATAGTTTAGAACTAGCCCTTTGCCCTTTGCAGTTTACAGTTTTAGGTAAACAAGCCTCATGCTGATCTTGAAATTTAAAATTTGTAGCCACTGATGCTCATTGTTCCTGATGTCCAAGATGTTTATACTCCTTTGCAAACTGATGTAATTGTTGGACTATCCGAGGTTAGTTTCAAATATTTTCGTGCTTGCAGAAGGTTACTAGATAAATTGATTACTTCGTTTTCTTTTCTTATTTTCGGTTTAGCCAATATGGTTAATTGGTTTGCTTCTTTTATGCTTAGTGCCGTCAACATTTAGAATTACTTCTCGAAAGCATTCCTACCATGTTCCAAAGTAATAGAACTTCTGAATCGGCCTTTGGTGCAGCAATCAAGGTATTGCGTTTTGCTTTAATGTTATAATAGTTGTCTGACTTTGCAATGTTTTTTATGCTGTTTGCTGAGATGGAATCAACTTTTTTTTTTTTTTTTTTATGAACGTTCATATAGTTTCTCGCTATAACTCCACTCTATAATTTCTTCATTTTCTTCTTTGCTTCCTTTCTGCATTTCTGAGATTTATTTTTCACGCAAGATTTTACTTTTTGTATGTAGAGTGTCTCTGTGATGTGTACTACTTACGTTTCATAGGGTGTCTTTTTAGTGTGTGCAGTTTTTTTTTTTTTTTTTTTTAAATTTAAATGATTTATGTTTTGGGTCAATGTGGATGCCTCATTGCTAGGAACCTACATTAGAAACAGAGGGAAAAAATAAATAAAAAATGATTTTATTGTTGGACAGTAAATAAATATATTAGGAAACAGAAATTTCCCAAGATTTTCCCGTCCGGAAAGGTTTCCCATTTTACAATTGAACACTTGCTTGGTTCTCTCGAAAGACATTGCCAAATGATCTCCCTACATACTTCCCTCTATCTAATAGGTTAGAGAGAAAAATGTTGATACTTTCTCCTCCCACAACTCATTAACCAACTAACTGCCTCCCTCTACCTTATCTGCCACTATTTGTGGCAGTCTAACTGAGCCACTGTAACAGCTTACATAGAAACATGGGCTGGCCCGCTTAATTTCTTGGACCCGTCCTTGTTTCTCCTGTCATTGCTTTGTTCTTTATTCCTCTACTGGTATTTATAATTCTTTCTGTTTGGGATTTCTATCTCGCAATTTTTGGCATTTGTTGTTACTTACCGTCTTCTCACTGAGGTTGACAAAAAAAGTATACATTTTGCCATTTAATAGATATAGTTTTTGACACCCATTCCTCTAACCAGCAACAATATTGATGCTGATACCTTTGTTTTCTTCTTTTAGGCGCTTTCCTTGCAATGA
Protein sequence

>MS.gene74958.t1

MAAPVPPGAPRPGSQPPPPNYVPNYRPNSDGLADNFNNLNLNRPPMTSNPVARPPPFAQQPPFPSSGPGIHASQPPFSRPGPPPGALVRPGGGPPSGGPPNAGPPGRPTGPPPGQPSPFGSRPTGPPGSFAAPVSGGVGVPPPGGSPPLRPSGPPPQNFGARPSLSPFNAPPSQSPFNAPLSQSPFNAPPTSAPPGMPPTSVPPSSLLSNGPPAFSGGAMPGPPRFPGGGVQQPPLGPPTMRTPLAPSAQSPFNMAPPAQSPFNMAPPQGIMQPPSSPFGAPSWQTQQQQQLVPPPTVPGSAQPPRMFGMPPPLPNQSMTTTISPAVGQTGAPMAGPSKIDPNQIPRPTPGTSVILHETRQGNQATIPPPATSDYIVRDTGNCSPRYMKCTVNQIPFTAELLTTSGMQLAMLVQPLALPHPSEEPIQVVDFGESGPVRCSRCKAYVNPFMKFIDQGRRFICNLCGFSDETPRDYHCNLGPDGRRRDADEKPELCRGTVEFVATKEFMVREPMPAVYFFLIDVSMNAVQTGATAAACSAISQVITDLPEGPNTKVGVATFDSTIHFYNLKRALQQPLMLIVPDVQDVYTPLQTDVIVGLSECRQHLELLLESIPTMFQSNRTSESAFGAAIKALSLQ