Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052361.t1 | RHN61694.1 | 99.8 | 434 | 1 | 0 | 1 | 434 | 28 | 461 | 7.30E-254 | 885.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052361.t1 | Q9LTR9 | 79.0 | 434 | 91 | 0 | 1 | 434 | 1 | 434 | 1.7e-206 | 719.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052361.t1 | A0A072UMM5 | 99.8 | 434 | 1 | 0 | 1 | 434 | 1 | 434 | 5.3e-254 | 885.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050169 | MS.gene052361 | 0.842017 | 3.34E-58 | -1.69E-46 |
| MS.gene050330 | MS.gene052361 | 0.843718 | 1.18E-58 | -1.69E-46 |
| MS.gene050787 | MS.gene052361 | -0.804988 | 1.66E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene052361 | 0.815196 | 1.05E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene052361 | 0.944954 | 7.52E-104 | -1.69E-46 |
| MS.gene052361 | MS.gene053054 | 0.838064 | 3.59E-57 | -1.69E-46 |
| MS.gene052361 | MS.gene054017 | 0.825801 | 3.85E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene054291 | 0.807444 | 5.04E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene056969 | 0.807603 | 4.66E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene056971 | 0.83019 | 3.38E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene060137 | 0.807944 | 3.95E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene060634 | 0.811129 | 8.17E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene061040 | 0.856244 | 3.67E-62 | -1.69E-46 |
| MS.gene052361 | MS.gene061114 | 0.819914 | 9.04E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene061523 | 0.819981 | 8.73E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene061658 | 0.814189 | 1.75E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene061900 | -0.801852 | 7.40E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene064489 | 0.83147 | 1.64E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene064675 | 0.813769 | 2.16E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene064797 | 0.819172 | 1.34E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene065390 | 0.832476 | 9.25E-56 | -1.69E-46 |
| MS.gene052361 | MS.gene06895 | 0.830869 | 2.30E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene069868 | 0.808948 | 2.41E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene071579 | 0.927912 | 6.05E-92 | -1.69E-46 |
| MS.gene052361 | MS.gene071605 | 0.841232 | 5.38E-58 | -1.69E-46 |
| MS.gene052361 | MS.gene071608 | 0.817806 | 2.72E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene071693 | 0.845971 | 2.91E-59 | -1.69E-46 |
| MS.gene052361 | MS.gene071790 | 0.803227 | 3.85E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene072397 | 0.831651 | 1.48E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene072584 | -0.810838 | 9.44E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene07424 | 0.807894 | 4.04E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene09417 | -0.82358 | 1.28E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene09796 | 0.827762 | 1.31E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene21312 | 0.803777 | 2.97E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene21597 | 0.844734 | 6.29E-59 | -1.69E-46 |
| MS.gene052361 | MS.gene22311 | 0.830992 | 2.15E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene22476 | 0.819908 | 9.07E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene23509 | 0.830618 | 2.65E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene23644 | -0.80349 | 3.40E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene23681 | -0.805068 | 1.60E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene23795 | 0.860132 | 2.56E-63 | -1.69E-46 |
| MS.gene052361 | MS.gene24760 | 0.829713 | 4.41E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene27239 | 0.850208 | 1.97E-60 | -1.69E-46 |
| MS.gene052361 | MS.gene28875 | 0.801357 | 9.35E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene28922 | 0.846689 | 1.86E-59 | -1.69E-46 |
| MS.gene052361 | MS.gene32189 | -0.810131 | 1.34E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene33127 | 0.811102 | 8.28E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene35437 | 0.842514 | 2.47E-58 | -1.69E-46 |
| MS.gene052361 | MS.gene35816 | 0.828356 | 9.41E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene35817 | 0.82619 | 3.11E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene35819 | 0.82239 | 2.43E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene35820 | 0.816768 | 4.66E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene37722 | 0.856738 | 2.63E-62 | -1.69E-46 |
| MS.gene052361 | MS.gene40837 | 0.811681 | 6.20E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene41707 | 0.820376 | 7.09E-53 | -1.69E-46 |
| MS.gene052361 | MS.gene43816 | -0.803865 | 2.84E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene44213 | 0.824527 | 7.69E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene44214 | 0.802036 | 6.79E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene44474 | 0.81208 | 5.07E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene44841 | 0.817158 | 3.81E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene45735 | -0.843316 | 1.51E-58 | -1.69E-46 |
| MS.gene052361 | MS.gene50093 | 0.817171 | 3.79E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene51549 | 0.869898 | 2.19E-66 | -1.69E-46 |
| MS.gene052361 | MS.gene51893 | 0.818447 | 1.95E-52 | -1.69E-46 |
| MS.gene052361 | MS.gene53875 | 0.840165 | 1.02E-57 | -1.69E-46 |
| MS.gene052361 | MS.gene54446 | 0.873297 | 1.64E-67 | -1.69E-46 |
| MS.gene052361 | MS.gene56530 | -0.803811 | 2.92E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene57577 | 0.801205 | 1.01E-48 | -1.69E-46 |
| MS.gene052361 | MS.gene57835 | 0.807714 | 4.42E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene61547 | 0.832708 | 8.10E-56 | -1.69E-46 |
| MS.gene052361 | MS.gene62687 | 0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene64000 | 0.848592 | 5.56E-60 | -1.69E-46 |
| MS.gene052361 | MS.gene65084 | 0.828958 | 6.73E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene66082 | 0.829713 | 4.41E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene68333 | -0.803762 | 2.99E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene74958 | 0.82645 | 2.70E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene75619 | 0.884979 | 1.21E-71 | -1.69E-46 |
| MS.gene052361 | MS.gene76533 | 0.809508 | 1.83E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene76773 | 0.803843 | 2.87E-49 | -1.69E-46 |
| MS.gene052361 | MS.gene77452 | 0.872112 | 4.08E-67 | -1.69E-46 |
| MS.gene052361 | MS.gene77584 | 0.807205 | 5.66E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene77682 | 0.800018 | 1.75E-48 | -1.69E-46 |
| MS.gene052361 | MS.gene81861 | 0.828957 | 6.73E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene85149 | 0.815034 | 1.14E-51 | -1.69E-46 |
| MS.gene052361 | MS.gene88672 | 0.825938 | 3.57E-54 | -1.69E-46 |
| MS.gene052361 | MS.gene94818 | 0.806657 | 7.40E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene95865 | 0.831888 | 1.29E-55 | -1.69E-46 |
| MS.gene052361 | MS.gene97579 | 0.808706 | 2.71E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene97883 | 0.810723 | 1.00E-50 | -1.69E-46 |
| MS.gene052361 | MS.gene98121 | 0.840767 | 7.13E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052361.t1 | MTR_4g077300 | 99.770 | 434 | 1 | 0 | 1 | 434 | 1 | 434 | 0.0 | 905 |
| MS.gene052361.t1 | MTR_4g077300 | 99.770 | 434 | 1 | 0 | 1 | 434 | 6 | 439 | 0.0 | 905 |
| MS.gene052361.t1 | MTR_2g040010 | 80.907 | 419 | 76 | 1 | 20 | 434 | 4 | 422 | 0.0 | 722 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052361.t1 | AT5G57020 | 79.032 | 434 | 91 | 0 | 1 | 434 | 1 | 434 | 0.0 | 738 |
| MS.gene052361.t1 | AT2G44175 | 42.391 | 92 | 48 | 1 | 28 | 119 | 10 | 96 | 2.51e-18 | 80.9 |
Find 110 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGACCAAAGACATAAATTT+TGG | 0.100981 | 4.4:+33313630 | MS.gene052361:CDS |
| GCTTTCAAAGTTGAGTAATT+TGG | 0.173591 | 4.4:-33314541 | None:intergenic |
| TCAGTGACCTCGTGAGTTTC+AGG | 0.204412 | 4.4:-33314475 | None:intergenic |
| TTTCCCAAAATTTATGTCTT+TGG | 0.247451 | 4.4:-33313634 | None:intergenic |
| ACCGACATCAATTAGTTTCT+TGG | 0.257794 | 4.4:-33314215 | None:intergenic |
| CTGCGGTTACAAGGCTAATT+AGG | 0.267951 | 4.4:+33314347 | MS.gene052361:CDS |
| CTTTCAAAGTTGAGTAATTT+GGG | 0.299469 | 4.4:-33314540 | None:intergenic |
| TCAGCAGGTGCATTTCCATC+TGG | 0.302312 | 4.4:-33313554 | None:intergenic |
| TATAAAGGCAACCATCTTCT+TGG | 0.307468 | 4.4:-33313963 | None:intergenic |
| TGAAGTAGCCCGGAGGTTGC+AGG | 0.313936 | 4.4:-33313907 | None:intergenic |
| CCGTGATGAGGTTGTTAATA+TGG | 0.329122 | 4.4:+33314012 | MS.gene052361:CDS |
| CCTCATCACGGACTCGAATT+CGG | 0.339678 | 4.4:-33314000 | None:intergenic |
| TTCTTGAAGGAACTGAAGTT+TGG | 0.345695 | 4.4:+33314691 | MS.gene052361:CDS |
| GCGCTGGGCCCTGCAACCTC+CGG | 0.353029 | 4.4:+33313898 | MS.gene052361:CDS |
| AAGATGGTTGCCTTTATAAC+AGG | 0.354888 | 4.4:+33313968 | MS.gene052361:CDS |
| CCTGGTGTGACTGTTGATTC+TGG | 0.363701 | 4.4:-33314295 | None:intergenic |
| GAATGTTGTCGATAGTTATC+TGG | 0.365092 | 4.4:+33314444 | MS.gene052361:CDS |
| ATTCTCCAAGTGCACCCTTC+TGG | 0.370742 | 4.4:-33314110 | None:intergenic |
| TGGAGCATTGGCTTCTTCCT+AGG | 0.375688 | 4.4:+33314419 | MS.gene052361:CDS |
| CTCATCACGGACTCGAATTC+GGG | 0.375743 | 4.4:-33313999 | None:intergenic |
| TACTTCACAGCTTGGCATAT+TGG | 0.377050 | 4.4:+33313923 | MS.gene052361:CDS |
| CAGCAGGTGCATTTCCATCT+GGG | 0.382043 | 4.4:-33313553 | None:intergenic |
| CCGACATCAATTAGTTTCTT+GGG | 0.382092 | 4.4:-33314214 | None:intergenic |
| GGGTGCACTTGGAGAATATA+TGG | 0.383844 | 4.4:+33314116 | MS.gene052361:CDS |
| AAGATGATGAGAACATGTTT+AGG | 0.387407 | 4.4:+33313852 | MS.gene052361:CDS |
| CAGTAGCAACTTGCCAATAC+TGG | 0.390437 | 4.4:+33314176 | MS.gene052361:CDS |
| GGTGCATTTCCATCTGGGTT+GGG | 0.393200 | 4.4:-33313548 | None:intergenic |
| GGCAGGGATACCTGTTATAA+AGG | 0.401328 | 4.4:-33313978 | None:intergenic |
| GTTTCTCTCGGCTCGGTGCT+CGG | 0.405888 | 4.4:+33314236 | MS.gene052361:CDS |
| GTTGGGCAGTTCAAGGATAT+AGG | 0.408153 | 4.4:+33313665 | MS.gene052361:CDS |
| CCCCTTATCTGAGGTAAAAC+AGG | 0.409550 | 4.4:+33313727 | MS.gene052361:CDS |
| CGCTGGGCCCTGCAACCTCC+GGG | 0.413953 | 4.4:+33313899 | MS.gene052361:CDS |
| GCAAAATGAAAGCTTCTTGA+AGG | 0.425222 | 4.4:+33314678 | MS.gene052361:CDS |
| TGTTAAAACTTCCAAGAAGA+TGG | 0.425379 | 4.4:+33313952 | MS.gene052361:CDS |
| ACCTTCCTAACCTCTATGAA+TGG | 0.426427 | 4.4:+33313759 | MS.gene052361:CDS |
| ATATCCTTGAACTGCCCAAC+AGG | 0.427798 | 4.4:-33313662 | None:intergenic |
| TTGGGCAGTTCAAGGATATA+GGG | 0.438419 | 4.4:+33313666 | MS.gene052361:CDS |
| ATCTCTACAACTACCGAGTA+AGG | 0.445048 | 4.4:+33314740 | MS.gene052361:CDS |
| ACACTTCCTTCTAGTATCCT+TGG | 0.449112 | 4.4:+33314514 | MS.gene052361:CDS |
| CAACATAATTATTAGCAAGA+AGG | 0.456558 | 4.4:-33313829 | None:intergenic |
| GGTGGTTCGATAGGACCTTC+AGG | 0.457527 | 4.4:-33313701 | None:intergenic |
| TAGAGGTTAGGAAGGTTGTA+TGG | 0.462978 | 4.4:-33313752 | None:intergenic |
| CATTGAAGTCATCAGAGCTT+GGG | 0.467422 | 4.4:+33314767 | MS.gene052361:CDS |
| GTTGATAGCAATCCTTCCTC+TGG | 0.471116 | 4.4:+33313503 | MS.gene052361:CDS |
| GGGTTGCCAAGGATACTAGA+AGG | 0.472607 | 4.4:-33314520 | None:intergenic |
| TCAACCTGTTGGGCAGTTCA+AGG | 0.473546 | 4.4:+33313658 | MS.gene052361:CDS |
| TTCGAAGGAATTTCTGCGCT+GGG | 0.475576 | 4.4:+33313883 | MS.gene052361:CDS |
| AACATAATTATTAGCAAGAA+GGG | 0.476139 | 4.4:-33313828 | None:intergenic |
| CACACCAGGGTTTAGAAAGA+TGG | 0.478975 | 4.4:+33314309 | MS.gene052361:CDS |
| AGGTGCATTTCCATCTGGGT+TGG | 0.483616 | 4.4:-33313549 | None:intergenic |
| TTTGGGAAACTCAACCTGTT+GGG | 0.485400 | 4.4:+33313648 | MS.gene052361:CDS |
| CAGTATTGGCAAGTTGCTAC+TGG | 0.490516 | 4.4:-33314175 | None:intergenic |
| TCAAAGATCTGTGCCAGTAT+TGG | 0.491976 | 4.4:-33314189 | None:intergenic |
| GTCACCTCTTTGATCATGAC+AGG | 0.495174 | 4.4:-33314088 | None:intergenic |
| GTAACCCATTCATAGAGGTT+AGG | 0.503092 | 4.4:-33313764 | None:intergenic |
| CATTCATCAGCTGAAGCAAA+GGG | 0.503326 | 4.4:-33314597 | None:intergenic |
| GGTGACCAGAAGGGTGCACT+TGG | 0.504257 | 4.4:+33314105 | MS.gene052361:CDS |
| TTGAGTAATTTGGGTTGCCA+AGG | 0.505565 | 4.4:-33314531 | None:intergenic |
| ATGATGTTCCTGCGGTTACA+AGG | 0.508314 | 4.4:+33314338 | MS.gene052361:CDS |
| CCTTCCTAACCTCTATGAAT+GGG | 0.513056 | 4.4:+33313760 | MS.gene052361:CDS |
| GGAGCATTGGCTTCTTCCTA+GGG | 0.513324 | 4.4:+33314420 | MS.gene052361:CDS |
| GCATTGAAGTCATCAGAGCT+TGG | 0.516250 | 4.4:+33314766 | MS.gene052361:CDS |
| ATTCGAAGGAATTTCTGCGC+TGG | 0.519044 | 4.4:+33313882 | MS.gene052361:CDS |
| CTCCGGGCTACTTCACAGCT+TGG | 0.520105 | 4.4:+33313915 | MS.gene052361:CDS |
| TCACGGACTCGAATTCGGGC+AGG | 0.527541 | 4.4:-33313995 | None:intergenic |
| CCCATTCATAGAGGTTAGGA+AGG | 0.528278 | 4.4:-33313760 | None:intergenic |
| TGCTGAAAGTGATCTTGCAT+TGG | 0.528701 | 4.4:+33313571 | MS.gene052361:CDS |
| ACGAAAATGATGTGGAGCAT+TGG | 0.530628 | 4.4:+33314407 | MS.gene052361:CDS |
| TGGCAAGTTGCTACTGGTGT+AGG | 0.531802 | 4.4:-33314169 | None:intergenic |
| CATGACAGGAGCAAGCCTCT+TGG | 0.536419 | 4.4:-33314074 | None:intergenic |
| TAATTGATGTCGGTTTCTCT+CGG | 0.541266 | 4.4:+33314224 | MS.gene052361:CDS |
| ACTTCAATGCATGCCTTACT+CGG | 0.542030 | 4.4:-33314753 | None:intergenic |
| GAAGTAGCCCGGAGGTTGCA+GGG | 0.543684 | 4.4:-33313906 | None:intergenic |
| TCATTCATCAGCTGAAGCAA+AGG | 0.547013 | 4.4:-33314598 | None:intergenic |
| ATTTCCATCTTTCTAAACCC+TGG | 0.548222 | 4.4:-33314313 | None:intergenic |
| CACGGACTCGAATTCGGGCA+GGG | 0.550332 | 4.4:-33313994 | None:intergenic |
| AAGAATCACTCCTGCTGTGT+AGG | 0.552278 | 4.4:-33314146 | None:intergenic |
| GATGTCGGTTTCTCTCGGCT+CGG | 0.552576 | 4.4:+33314229 | MS.gene052361:CDS |
| TGCCAAGCTGTGAAGTAGCC+CGG | 0.554188 | 4.4:-33313917 | None:intergenic |
| CTAATTAGCCTTGTAACCGC+AGG | 0.559185 | 4.4:-33314346 | None:intergenic |
| CCAGAATCAACAGTCACACC+AGG | 0.560206 | 4.4:+33314295 | MS.gene052361:CDS |
| TTGTAACCGCAGGAACATCA+TGG | 0.561294 | 4.4:-33314336 | None:intergenic |
| CCCAAGAAACTAATTGATGT+CGG | 0.561835 | 4.4:+33314214 | MS.gene052361:CDS |
| ATAGGACCTTCAGGCAAAGT+GGG | 0.564234 | 4.4:-33313692 | None:intergenic |
| TGGCAGGCTGCCTACACAGC+AGG | 0.566798 | 4.4:+33314136 | MS.gene052361:CDS |
| CTATCGACAACATTCTCCCT+AGG | 0.573804 | 4.4:-33314436 | None:intergenic |
| TGATCAAAGAGGTGACCAGA+AGG | 0.575360 | 4.4:+33314095 | MS.gene052361:CDS |
| AATGCAAGATCACTTTCAGC+AGG | 0.575390 | 4.4:-33313569 | None:intergenic |
| CACAAGTAACCCATTCATAG+AGG | 0.576099 | 4.4:-33313769 | None:intergenic |
| ACTCAAAATCCCAACCCAGA+TGG | 0.582100 | 4.4:+33313539 | MS.gene052361:CDS |
| CTGAAGTTTGGACCAGGTGA+TGG | 0.583002 | 4.4:+33314703 | MS.gene052361:CDS |
| GTTTAGGTTTAACTATTCGA+AGG | 0.584434 | 4.4:+33313868 | MS.gene052361:CDS |
| GATAGGACCTTCAGGCAAAG+TGG | 0.591235 | 4.4:-33313693 | None:intergenic |
| GCACTTGGAGAATATATGGC+AGG | 0.591925 | 4.4:+33314120 | MS.gene052361:CDS |
| GGGAACCCCACTTTGCCTGA+AGG | 0.595352 | 4.4:+33313686 | MS.gene052361:CDS |
| AAGGAACTGAAGTTTGGACC+AGG | 0.609351 | 4.4:+33314697 | MS.gene052361:CDS |
| TCTTCAGGTGATCCAGAGGA+AGG | 0.613470 | 4.4:-33313515 | None:intergenic |
| TAATGAAGTTTACCATCACC+TGG | 0.622265 | 4.4:-33314715 | None:intergenic |
| CCATATTAACAACCTCATCA+CGG | 0.625479 | 4.4:-33314012 | None:intergenic |
| ACAAGAAGCTTCGAACCAAG+AGG | 0.628890 | 4.4:+33314059 | MS.gene052361:CDS |
| GATCAAAGAGGTGACCAGAA+GGG | 0.633175 | 4.4:+33314096 | MS.gene052361:CDS |
| CCGAATTCGAGTCCGTGATG+AGG | 0.640464 | 4.4:+33314000 | MS.gene052361:CDS |
| AGTCTCTTCAGGTGATCCAG+AGG | 0.644950 | 4.4:-33313519 | None:intergenic |
| TGCTCCTGTCATGATCAAAG+AGG | 0.676350 | 4.4:+33314084 | MS.gene052361:CDS |
| GGAAATCCATGATGTTCCTG+CGG | 0.677320 | 4.4:+33314330 | MS.gene052361:CDS |
| TAGGACCTTCAGGCAAAGTG+GGG | 0.681811 | 4.4:-33313691 | None:intergenic |
| CAGAATCAACAGTCACACCA+GGG | 0.712272 | 4.4:+33314296 | MS.gene052361:CDS |
| ATTCATCAGCTGAAGCAAAG+GGG | 0.718075 | 4.4:-33314596 | None:intergenic |
| TGAAAGTCCTGAAACTCACG+AGG | 0.726533 | 4.4:+33314468 | MS.gene052361:CDS |
| CAAGCTGTGAAGTAGCCCGG+AGG | 0.761817 | 4.4:-33313914 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATAATTATTAGCAAGAA+GGG | - | chr4.4:33313831-33313850 | None:intergenic | 20.0% |
| ! | CAACATAATTATTAGCAAGA+AGG | - | chr4.4:33313832-33313851 | None:intergenic | 25.0% |
| ! | CTTTCAAAGTTGAGTAATTT+GGG | - | chr4.4:33314543-33314562 | None:intergenic | 25.0% |
| ! | TTTCCCAAAATTTATGTCTT+TGG | - | chr4.4:33313637-33313656 | None:intergenic | 25.0% |
| AAGATGATGAGAACATGTTT+AGG | + | chr4.4:33313852-33313871 | MS.gene052361:CDS | 30.0% | |
| GCTTTCAAAGTTGAGTAATT+TGG | - | chr4.4:33314544-33314563 | None:intergenic | 30.0% | |
| TGTTAAAACTTCCAAGAAGA+TGG | + | chr4.4:33313952-33313971 | MS.gene052361:CDS | 30.0% | |
| ! | ACATCATTTTCGTCAAAATC+AGG | - | chr4.4:33314400-33314419 | None:intergenic | 30.0% |
| ! | CGACCAAAGACATAAATTTT+GGG | + | chr4.4:33313631-33313650 | MS.gene052361:CDS | 30.0% |
| ! | GTTTAGGTTTAACTATTCGA+AGG | + | chr4.4:33313868-33313887 | MS.gene052361:CDS | 30.0% |
| ! | TCGACCAAAGACATAAATTT+TGG | + | chr4.4:33313630-33313649 | MS.gene052361:CDS | 30.0% |
| ! | TTATGATGTTTTCAACGCTT+TGG | + | chr4.4:33314648-33314667 | MS.gene052361:CDS | 30.0% |
| !! | TGATTTTGACGAAAATGATG+TGG | + | chr4.4:33314399-33314418 | MS.gene052361:CDS | 30.0% |
| AAGATGGTTGCCTTTATAAC+AGG | + | chr4.4:33313968-33313987 | MS.gene052361:CDS | 35.0% | |
| ATTTCCATCTTTCTAAACCC+TGG | - | chr4.4:33314316-33314335 | None:intergenic | 35.0% | |
| CCATATTAACAACCTCATCA+CGG | - | chr4.4:33314015-33314034 | None:intergenic | 35.0% | |
| CCCAAGAAACTAATTGATGT+CGG | + | chr4.4:33314214-33314233 | MS.gene052361:CDS | 35.0% | |
| GAATGTTGTCGATAGTTATC+TGG | + | chr4.4:33314444-33314463 | MS.gene052361:CDS | 35.0% | |
| GCAAAATGAAAGCTTCTTGA+AGG | + | chr4.4:33314678-33314697 | MS.gene052361:CDS | 35.0% | |
| TAATGAAGTTTACCATCACC+TGG | - | chr4.4:33314718-33314737 | None:intergenic | 35.0% | |
| TATAAAGGCAACCATCTTCT+TGG | - | chr4.4:33313966-33313985 | None:intergenic | 35.0% | |
| TTCTTGAAGGAACTGAAGTT+TGG | + | chr4.4:33314691-33314710 | MS.gene052361:CDS | 35.0% | |
| ! | ACCGACATCAATTAGTTTCT+TGG | - | chr4.4:33314218-33314237 | None:intergenic | 35.0% |
| ! | CCGACATCAATTAGTTTCTT+GGG | - | chr4.4:33314217-33314236 | None:intergenic | 35.0% |
| ! | TCCTGTTTTACCTCAGATAA+GGG | - | chr4.4:33313731-33313750 | None:intergenic | 35.0% |
| ! | TTCCTGTTTTACCTCAGATA+AGG | - | chr4.4:33313732-33313751 | None:intergenic | 35.0% |
| !! | TAATTGATGTCGGTTTCTCT+CGG | + | chr4.4:33314224-33314243 | MS.gene052361:CDS | 35.0% |
| AATGCAAGATCACTTTCAGC+AGG | - | chr4.4:33313572-33313591 | None:intergenic | 40.0% | |
| ACACTTCCTTCTAGTATCCT+TGG | + | chr4.4:33314514-33314533 | MS.gene052361:CDS | 40.0% | |
| ACCTTCCTAACCTCTATGAA+TGG | + | chr4.4:33313759-33313778 | MS.gene052361:CDS | 40.0% | |
| ACGAAAATGATGTGGAGCAT+TGG | + | chr4.4:33314407-33314426 | MS.gene052361:CDS | 40.0% | |
| ACTTCAATGCATGCCTTACT+CGG | - | chr4.4:33314756-33314775 | None:intergenic | 40.0% | |
| ATCTCTACAACTACCGAGTA+AGG | + | chr4.4:33314740-33314759 | MS.gene052361:CDS | 40.0% | |
| ATTCATCAGCTGAAGCAAAG+GGG | - | chr4.4:33314599-33314618 | None:intergenic | 40.0% | |
| CACAAGTAACCCATTCATAG+AGG | - | chr4.4:33313772-33313791 | None:intergenic | 40.0% | |
| CATTCATCAGCTGAAGCAAA+GGG | - | chr4.4:33314600-33314619 | None:intergenic | 40.0% | |
| CATTGAAGTCATCAGAGCTT+GGG | + | chr4.4:33314767-33314786 | MS.gene052361:CDS | 40.0% | |
| CCGTGATGAGGTTGTTAATA+TGG | + | chr4.4:33314012-33314031 | MS.gene052361:CDS | 40.0% | |
| CCTTCCTAACCTCTATGAAT+GGG | + | chr4.4:33313760-33313779 | MS.gene052361:CDS | 40.0% | |
| GTAACCCATTCATAGAGGTT+AGG | - | chr4.4:33313767-33313786 | None:intergenic | 40.0% | |
| TACTTCACAGCTTGGCATAT+TGG | + | chr4.4:33313923-33313942 | MS.gene052361:CDS | 40.0% | |
| TAGAGGTTAGGAAGGTTGTA+TGG | - | chr4.4:33313755-33313774 | None:intergenic | 40.0% | |
| TCAAAGATCTGTGCCAGTAT+TGG | - | chr4.4:33314192-33314211 | None:intergenic | 40.0% | |
| TCATTCATCAGCTGAAGCAA+AGG | - | chr4.4:33314601-33314620 | None:intergenic | 40.0% | |
| TGCTGAAAGTGATCTTGCAT+TGG | + | chr4.4:33313571-33313590 | MS.gene052361:CDS | 40.0% | |
| TTGGGCAGTTCAAGGATATA+GGG | + | chr4.4:33313666-33313685 | MS.gene052361:CDS | 40.0% | |
| TTTGGGAAACTCAACCTGTT+GGG | + | chr4.4:33313648-33313667 | MS.gene052361:CDS | 40.0% | |
| ! | CCTGTTTTACCTCAGATAAG+GGG | - | chr4.4:33313730-33313749 | None:intergenic | 40.0% |
| ! | CTGTTTTACCTCAGATAAGG+GGG | - | chr4.4:33313729-33313748 | None:intergenic | 40.0% |
| !! | TTGAGTAATTTGGGTTGCCA+AGG | - | chr4.4:33314534-33314553 | None:intergenic | 40.0% |
| !! | TTGGGATTTTGAGTCTCTTC+AGG | - | chr4.4:33313533-33313552 | None:intergenic | 40.0% |
| !! | TTTTGGGAAACTCAACCTGT+TGG | + | chr4.4:33313647-33313666 | MS.gene052361:CDS | 40.0% |
| AAGAATCACTCCTGCTGTGT+AGG | - | chr4.4:33314149-33314168 | None:intergenic | 45.0% | |
| AAGGAACTGAAGTTTGGACC+AGG | + | chr4.4:33314697-33314716 | MS.gene052361:CDS | 45.0% | |
| ACAAGAAGCTTCGAACCAAG+AGG | + | chr4.4:33314059-33314078 | MS.gene052361:CDS | 45.0% | |
| ACTCAAAATCCCAACCCAGA+TGG | + | chr4.4:33313539-33313558 | MS.gene052361:CDS | 45.0% | |
| ATAGGACCTTCAGGCAAAGT+GGG | - | chr4.4:33313695-33313714 | None:intergenic | 45.0% | |
| ATATCCTTGAACTGCCCAAC+AGG | - | chr4.4:33313665-33313684 | None:intergenic | 45.0% | |
| ATGATGTTCCTGCGGTTACA+AGG | + | chr4.4:33314338-33314357 | MS.gene052361:CDS | 45.0% | |
| ATTCGAAGGAATTTCTGCGC+TGG | + | chr4.4:33313882-33313901 | MS.gene052361:CDS | 45.0% | |
| CACACCAGGGTTTAGAAAGA+TGG | + | chr4.4:33314309-33314328 | MS.gene052361:CDS | 45.0% | |
| CAGAATCAACAGTCACACCA+GGG | + | chr4.4:33314296-33314315 | MS.gene052361:CDS | 45.0% | |
| CAGTAGCAACTTGCCAATAC+TGG | + | chr4.4:33314176-33314195 | MS.gene052361:CDS | 45.0% | |
| CCCATTCATAGAGGTTAGGA+AGG | - | chr4.4:33313763-33313782 | None:intergenic | 45.0% | |
| CTATCGACAACATTCTCCCT+AGG | - | chr4.4:33314439-33314458 | None:intergenic | 45.0% | |
| CTGCGGTTACAAGGCTAATT+AGG | + | chr4.4:33314347-33314366 | MS.gene052361:CDS | 45.0% | |
| GATCAAAGAGGTGACCAGAA+GGG | + | chr4.4:33314096-33314115 | MS.gene052361:CDS | 45.0% | |
| GCACTTGGAGAATATATGGC+AGG | + | chr4.4:33314120-33314139 | MS.gene052361:CDS | 45.0% | |
| GCATTGAAGTCATCAGAGCT+TGG | + | chr4.4:33314766-33314785 | MS.gene052361:CDS | 45.0% | |
| GGAAATCCATGATGTTCCTG+CGG | + | chr4.4:33314330-33314349 | MS.gene052361:CDS | 45.0% | |
| GGCAGGGATACCTGTTATAA+AGG | - | chr4.4:33313981-33314000 | None:intergenic | 45.0% | |
| GGGTGCACTTGGAGAATATA+TGG | + | chr4.4:33314116-33314135 | MS.gene052361:CDS | 45.0% | |
| GTCACCTCTTTGATCATGAC+AGG | - | chr4.4:33314091-33314110 | None:intergenic | 45.0% | |
| GTTGGGCAGTTCAAGGATAT+AGG | + | chr4.4:33313665-33313684 | MS.gene052361:CDS | 45.0% | |
| TGAAAGTCCTGAAACTCACG+AGG | + | chr4.4:33314468-33314487 | MS.gene052361:CDS | 45.0% | |
| TGATCAAAGAGGTGACCAGA+AGG | + | chr4.4:33314095-33314114 | MS.gene052361:CDS | 45.0% | |
| TGCTCCTGTCATGATCAAAG+AGG | + | chr4.4:33314084-33314103 | MS.gene052361:CDS | 45.0% | |
| TTCGAAGGAATTTCTGCGCT+GGG | + | chr4.4:33313883-33313902 | MS.gene052361:CDS | 45.0% | |
| TTGTAACCGCAGGAACATCA+TGG | - | chr4.4:33314339-33314358 | None:intergenic | 45.0% | |
| TTTACCTCAGATAAGGGGGT+TGG | - | chr4.4:33313725-33313744 | None:intergenic | 45.0% | |
| ! | CCCCTTATCTGAGGTAAAAC+AGG | + | chr4.4:33313727-33313746 | MS.gene052361:CDS | 45.0% |
| !! | CAGTATTGGCAAGTTGCTAC+TGG | - | chr4.4:33314178-33314197 | None:intergenic | 45.0% |
| !! | CTAATTAGCCTTGTAACCGC+AGG | - | chr4.4:33314349-33314368 | None:intergenic | 45.0% |
| !! | GTTGATAGCAATCCTTCCTC+TGG | + | chr4.4:33313503-33313522 | MS.gene052361:CDS | 45.0% |
| AGGTGCATTTCCATCTGGGT+TGG | - | chr4.4:33313552-33313571 | None:intergenic | 50.0% | |
| AGTCTCTTCAGGTGATCCAG+AGG | - | chr4.4:33313522-33313541 | None:intergenic | 50.0% | |
| ATTCTCCAAGTGCACCCTTC+TGG | - | chr4.4:33314113-33314132 | None:intergenic | 50.0% | |
| CAGCAGGTGCATTTCCATCT+GGG | - | chr4.4:33313556-33313575 | None:intergenic | 50.0% | |
| CCAGAATCAACAGTCACACC+AGG | + | chr4.4:33314295-33314314 | MS.gene052361:CDS | 50.0% | |
| CCTCATCACGGACTCGAATT+CGG | - | chr4.4:33314003-33314022 | None:intergenic | 50.0% | |
| CTCATCACGGACTCGAATTC+GGG | - | chr4.4:33314002-33314021 | None:intergenic | 50.0% | |
| CTGAAGTTTGGACCAGGTGA+TGG | + | chr4.4:33314703-33314722 | MS.gene052361:CDS | 50.0% | |
| GATAGGACCTTCAGGCAAAG+TGG | - | chr4.4:33313696-33313715 | None:intergenic | 50.0% | |
| GGGTTGCCAAGGATACTAGA+AGG | - | chr4.4:33314523-33314542 | None:intergenic | 50.0% | |
| GGTGCATTTCCATCTGGGTT+GGG | - | chr4.4:33313551-33313570 | None:intergenic | 50.0% | |
| TAGGACCTTCAGGCAAAGTG+GGG | - | chr4.4:33313694-33313713 | None:intergenic | 50.0% | |
| TCAACCTGTTGGGCAGTTCA+AGG | + | chr4.4:33313658-33313677 | MS.gene052361:CDS | 50.0% | |
| TCAGCAGGTGCATTTCCATC+TGG | - | chr4.4:33313557-33313576 | None:intergenic | 50.0% | |
| TCAGTGACCTCGTGAGTTTC+AGG | - | chr4.4:33314478-33314497 | None:intergenic | 50.0% | |
| TCTTCAGGTGATCCAGAGGA+AGG | - | chr4.4:33313518-33313537 | None:intergenic | 50.0% | |
| TGGCAAGTTGCTACTGGTGT+AGG | - | chr4.4:33314172-33314191 | None:intergenic | 50.0% | |
| !! | CCTGGTGTGACTGTTGATTC+TGG | - | chr4.4:33314298-33314317 | None:intergenic | 50.0% |
| !! | GGAGCATTGGCTTCTTCCTA+GGG | + | chr4.4:33314420-33314439 | MS.gene052361:CDS | 50.0% |
| !! | TGGAGCATTGGCTTCTTCCT+AGG | + | chr4.4:33314419-33314438 | MS.gene052361:CDS | 50.0% |
| ACCACCAACCCCCTTATCTG+AGG | + | chr4.4:33313718-33313737 | MS.gene052361:CDS | 55.0% | |
| ACCTCAGATAAGGGGGTTGG+TGG | - | chr4.4:33313722-33313741 | None:intergenic | 55.0% | |
| CATGACAGGAGCAAGCCTCT+TGG | - | chr4.4:33314077-33314096 | None:intergenic | 55.0% | |
| CCGAATTCGAGTCCGTGATG+AGG | + | chr4.4:33314000-33314019 | MS.gene052361:CDS | 55.0% | |
| GGTGGTTCGATAGGACCTTC+AGG | - | chr4.4:33313704-33313723 | None:intergenic | 55.0% | |
| TGCCAAGCTGTGAAGTAGCC+CGG | - | chr4.4:33313920-33313939 | None:intergenic | 55.0% | |
| !! | AAGGGGGTTGGTGGTTCGAT+AGG | - | chr4.4:33313713-33313732 | None:intergenic | 55.0% |
| !! | GATGTCGGTTTCTCTCGGCT+CGG | + | chr4.4:33314229-33314248 | MS.gene052361:CDS | 55.0% |
| CAAGCTGTGAAGTAGCCCGG+AGG | - | chr4.4:33313917-33313936 | None:intergenic | 60.0% | |
| CACGGACTCGAATTCGGGCA+GGG | - | chr4.4:33313997-33314016 | None:intergenic | 60.0% | |
| CTCCGGGCTACTTCACAGCT+TGG | + | chr4.4:33313915-33313934 | MS.gene052361:CDS | 60.0% | |
| GAAGTAGCCCGGAGGTTGCA+GGG | - | chr4.4:33313909-33313928 | None:intergenic | 60.0% | |
| GGGAACCCCACTTTGCCTGA+AGG | + | chr4.4:33313686-33313705 | MS.gene052361:CDS | 60.0% | |
| TCACGGACTCGAATTCGGGC+AGG | - | chr4.4:33313998-33314017 | None:intergenic | 60.0% | |
| TGAAGTAGCCCGGAGGTTGC+AGG | - | chr4.4:33313910-33313929 | None:intergenic | 60.0% | |
| ! | GGTGACCAGAAGGGTGCACT+TGG | + | chr4.4:33314105-33314124 | MS.gene052361:CDS | 60.0% |
| !! | GTTTCTCTCGGCTCGGTGCT+CGG | + | chr4.4:33314236-33314255 | MS.gene052361:CDS | 60.0% |
| TGGCAGGCTGCCTACACAGC+AGG | + | chr4.4:33314136-33314155 | MS.gene052361:CDS | 65.0% | |
| CGCTGGGCCCTGCAACCTCC+GGG | + | chr4.4:33313899-33313918 | MS.gene052361:CDS | 75.0% | |
| GCGCTGGGCCCTGCAACCTC+CGG | + | chr4.4:33313898-33313917 | MS.gene052361:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 33313500 | 33314804 | 33313500 | ID=MS.gene052361 |
| chr4.4 | mRNA | 33313500 | 33314804 | 33313500 | ID=MS.gene052361.t1;Parent=MS.gene052361 |
| chr4.4 | exon | 33313500 | 33314804 | 33313500 | ID=MS.gene052361.t1.exon1;Parent=MS.gene052361.t1 |
| chr4.4 | CDS | 33313500 | 33314804 | 33313500 | ID=cds.MS.gene052361.t1;Parent=MS.gene052361.t1 |
| Gene Sequence |
| Protein sequence |
