Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75194.t1 | AFK48706.1 | 93.6 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 1.20E-78 | 302.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75194.t1 | G8A288 | 93.6 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 8.6e-79 | 302.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene75194 | 0.804561 | 2.04E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene75194 | -0.819351 | 1.22E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene75194 | 0.801275 | 9.72E-49 | -1.69E-46 |
| MS.gene058435 | MS.gene75194 | 0.80642 | 8.30E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene75194 | 0.811598 | 6.46E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75194.t1 | MTR_1g109370 | 93.642 | 173 | 7 | 1 | 23 | 195 | 48 | 216 | 1.40e-112 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75194.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 62 | 215 | 4.32e-39 | 133 |
| MS.gene75194.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 62 | 215 | 4.32e-39 | 133 |
| MS.gene75194.t1 | AT1G68185 | 46.452 | 155 | 81 | 2 | 42 | 195 | 108 | 261 | 1.87e-38 | 133 |
Find 45 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTCATCAGTCTTCTTA+TGG | 0.228908 | 1.3:+74367227 | None:intergenic |
| ACCATCGAGGCACACGATAT+TGG | 0.263756 | 1.3:+74367623 | None:intergenic |
| AAAGCAGATTCGGATGTTTA+TGG | 0.264643 | 1.3:-74366541 | MS.gene75194:intron |
| CAAATTGTGTTGTCATTTGA+TGG | 0.307014 | 1.3:-74366214 | MS.gene75194:CDS |
| TATCATTATCTTCCATTTCA+AGG | 0.332434 | 1.3:+74366158 | None:intergenic |
| CACGGAAGGTTGCAATGAAT+TGG | 0.349044 | 1.3:+74366641 | None:intergenic |
| CCATCGAGGCACACGATATT+GGG | 0.373094 | 1.3:+74367624 | None:intergenic |
| AGTCTTCTTATGGGCCTTAC+CGG | 0.377421 | 1.3:+74367237 | None:intergenic |
| CTCCTCATCAGTCTTCTTAT+GGG | 0.387413 | 1.3:+74367228 | None:intergenic |
| AAACAAGGGTTCAAGTTCTT+CGG | 0.392297 | 1.3:+74367665 | None:intergenic |
| AACCCCTGCCAGCCTTGAAA+TGG | 0.394451 | 1.3:-74366170 | MS.gene75194:CDS |
| ATTCAACTTTGAAGAATTTG+AGG | 0.439470 | 1.3:-74367204 | MS.gene75194:intron |
| CCCAATATCGTGTGCCTCGA+TGG | 0.441844 | 1.3:-74367624 | MS.gene75194:intron |
| TTTGGGGTTTAGGTTGAGAA+TGG | 0.451301 | 1.3:-74367337 | MS.gene75194:intron |
| TCTTCCATTTCAAGGCTGGC+AGG | 0.456162 | 1.3:+74366166 | None:intergenic |
| AGGATGAAACAAAGCAGATT+CGG | 0.459454 | 1.3:-74366551 | MS.gene75194:CDS |
| TTCCATTTCAAGGCTGGCAG+GGG | 0.465493 | 1.3:+74366168 | None:intergenic |
| GCCTTACCGGTGACCCTTGG+TGG | 0.466237 | 1.3:+74367250 | None:intergenic |
| TGGGCCTTACCGGTGACCCT+TGG | 0.476360 | 1.3:+74367247 | None:intergenic |
| CAATTCATTGCAACCTTCCG+TGG | 0.476932 | 1.3:-74366640 | MS.gene75194:CDS |
| ATTATCTTCCATTTCAAGGC+TGG | 0.485099 | 1.3:+74366162 | None:intergenic |
| GCCACCAAGGGTCACCGGTA+AGG | 0.490738 | 1.3:-74367251 | MS.gene75194:CDS |
| CTTCCATTTCAAGGCTGGCA+GGG | 0.494291 | 1.3:+74366167 | None:intergenic |
| TTGGGGTTTAGGTTGAGAAT+GGG | 0.505216 | 1.3:-74367336 | MS.gene75194:intron |
| AGAATCTTTGCTCGTTCAGA+AGG | 0.520871 | 1.3:+74366591 | None:intergenic |
| TTTGTATAGATTGAAGAAGC+AGG | 0.529052 | 1.3:-74366736 | MS.gene75194:intron |
| TCACTTACGTCGTCCACGGA+AGG | 0.541348 | 1.3:+74366627 | None:intergenic |
| CCGGTGACCCTTGGTGGCGA+AGG | 0.548861 | 1.3:+74367256 | None:intergenic |
| TGTAGGATGACCAGTTTGAA+AGG | 0.550107 | 1.3:-74366279 | MS.gene75194:intron |
| CCTTCGCCACCAAGGGTCAC+CGG | 0.569098 | 1.3:-74367256 | MS.gene75194:CDS |
| TGAGAATGGGAAGAATACTA+AGG | 0.569816 | 1.3:-74367323 | MS.gene75194:CDS |
| TTGGTTACCTTCGCCACCAA+GGG | 0.579619 | 1.3:-74367263 | MS.gene75194:CDS |
| AACGCGACTGTAATCAAACA+AGG | 0.602179 | 1.3:+74367650 | None:intergenic |
| GAATGGGAAGAATACTAAGG+TGG | 0.613980 | 1.3:-74367320 | MS.gene75194:CDS |
| TGGACCTCGAGGATGATGAT+TGG | 0.623297 | 1.3:-74367282 | MS.gene75194:CDS |
| TCTCATCTCTGTTCAAGACA+AGG | 0.626807 | 1.3:-74366571 | MS.gene75194:CDS |
| CCATAAGAAGACTGATGAGG+AGG | 0.627181 | 1.3:-74367227 | MS.gene75194:CDS |
| ATTGGTTACCTTCGCCACCA+AGG | 0.629138 | 1.3:-74367264 | MS.gene75194:CDS |
| TAAGGTGGAAACAGTGAATG+TGG | 0.687527 | 1.3:-74367305 | MS.gene75194:CDS |
| GGCCCATAAGAAGACTGATG+AGG | 0.692280 | 1.3:-74367230 | MS.gene75194:CDS |
| AGTGAATGTGGTGGACCTCG+AGG | 0.707436 | 1.3:-74367293 | MS.gene75194:CDS |
| GGTGGAAACAGTGAATGTGG+TGG | 0.711849 | 1.3:-74367302 | MS.gene75194:CDS |
| TTAAGGAGTGCTAACCATCG+AGG | 0.734889 | 1.3:+74367610 | None:intergenic |
| ACGCGACTGTAATCAAACAA+GGG | 0.742183 | 1.3:+74367651 | None:intergenic |
| GTAACCAATCATCATCCTCG+AGG | 0.759157 | 1.3:+74367278 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAAAAATTAAACATT+TGG | + | chr1.3:74366249-74366268 | None:intergenic | 10.0% |
| !! | AAACAAAAAATTAAACATTT+GGG | + | chr1.3:74366248-74366267 | None:intergenic | 10.0% |
| !! | TTAATTTCAATTTGAATAAT+CGG | + | chr1.3:74366221-74366240 | None:intergenic | 10.0% |
| !! | AACAAAAAATTAAACATTTG+GGG | + | chr1.3:74366247-74366266 | None:intergenic | 15.0% |
| !! | CTGATAAAGAAAATAAAAAA+AGG | + | chr1.3:74367418-74367437 | None:intergenic | 15.0% |
| !!! | GTAAATTTTGATAATGTTTT+GGG | - | chr1.3:74366446-74366465 | MS.gene75194:intron | 15.0% |
| !!! | TAAATTTTGATAATGTTTTG+GGG | - | chr1.3:74366447-74366466 | MS.gene75194:intron | 15.0% |
| !!! | TGTAAATTTTGATAATGTTT+TGG | - | chr1.3:74366445-74366464 | MS.gene75194:intron | 15.0% |
| !! | AATGGCAAAAAACAAAAATT+TGG | + | chr1.3:74366659-74366678 | None:intergenic | 20.0% |
| !! | ACAAAAAATTAAACATTTGG+GGG | + | chr1.3:74366246-74366265 | None:intergenic | 20.0% |
| !! | ATGAGTAGAATAACTTATAT+TGG | + | chr1.3:74366973-74366992 | None:intergenic | 20.0% |
| !! | CAAAACAAAAAACACAAATT+TGG | + | chr1.3:74366398-74366417 | None:intergenic | 20.0% |
| !! | TAAAATAACATGCTCATAAA+GGG | - | chr1.3:74366840-74366859 | MS.gene75194:intron | 20.0% |
| !! | TAAAGAAAATAAAAAAAGGC+AGG | + | chr1.3:74367414-74367433 | None:intergenic | 20.0% |
| !! | TTAAAATAACATGCTCATAA+AGG | - | chr1.3:74366839-74366858 | MS.gene75194:intron | 20.0% |
| !!! | TTGTATAAAAAAAGTGTGTT+GGG | - | chr1.3:74366888-74366907 | MS.gene75194:intron | 20.0% |
| ! | ACATCTTATAAGAAACTGTA+TGG | + | chr1.3:74367038-74367057 | None:intergenic | 25.0% |
| ! | TAACTAATTAAGCCTATTCT+TGG | - | chr1.3:74366935-74366954 | MS.gene75194:intron | 25.0% |
| ! | TAAGTAAACAAACAATGCAA+TGG | + | chr1.3:74366677-74366696 | None:intergenic | 25.0% |
| ! | TATCATTATCTTCCATTTCA+AGG | + | chr1.3:74367645-74367664 | None:intergenic | 25.0% |
| ! | TGTTGATGTTCTTAATATTG+TGG | - | chr1.3:74367313-74367332 | MS.gene75194:CDS | 25.0% |
| ! | TTGAATAATCGGAATAGTTA+AGG | + | chr1.3:74366210-74366229 | None:intergenic | 25.0% |
| !! | AGTTTGTTTGAAATGGTATA+AGG | - | chr1.3:74367371-74367390 | MS.gene75194:intron | 25.0% |
| !! | ATTCAACTTTGAAGAATTTG+AGG | - | chr1.3:74366596-74366615 | MS.gene75194:CDS | 25.0% |
| !! | CTTGTATAAAAAAAGTGTGT+TGG | - | chr1.3:74366887-74366906 | MS.gene75194:intron | 25.0% |
| !! | GTTTGTTTGAAATGGTATAA+GGG | - | chr1.3:74367372-74367391 | MS.gene75194:intron | 25.0% |
| !!! | TAGTTGTAGTTTGTTTGAAA+TGG | - | chr1.3:74367364-74367383 | MS.gene75194:intron | 25.0% |
| !!! | TGTAGCAATGTTTTAAAAAC+CGG | + | chr1.3:74366805-74366824 | None:intergenic | 25.0% |
| !!! | TGTGTTTTTTGTTTTGTACA+AGG | - | chr1.3:74366402-74366421 | MS.gene75194:intron | 25.0% |
| !!! | TTTTCCGATTTGTTTGATTT+CGG | + | chr1.3:74366776-74366795 | None:intergenic | 25.0% |
| !!! | TTTTGATAATCCTTTCAAAC+TGG | + | chr1.3:74367534-74367553 | None:intergenic | 25.0% |
| AATTAAGCCTATTCTTGGTT+TGG | - | chr1.3:74366940-74366959 | MS.gene75194:intron | 30.0% | |
| ATCCTACAGAGAATTGAAAA+AGG | + | chr1.3:74367509-74367528 | None:intergenic | 30.0% | |
| GAATTCTTCTTTCTCTTCTT+AGG | + | chr1.3:74366344-74366363 | None:intergenic | 30.0% | |
| GTAACCGAAATCAAACAAAT+CGG | - | chr1.3:74366769-74366788 | MS.gene75194:intron | 30.0% | |
| TAAAGTTCCAAACCAAGAAT+AGG | + | chr1.3:74366950-74366969 | None:intergenic | 30.0% | |
| TTTGTATAGATTGAAGAAGC+AGG | - | chr1.3:74367064-74367083 | MS.gene75194:intron | 30.0% | |
| TTTGTTTACTTAACACTCCT+AGG | - | chr1.3:74366685-74366704 | MS.gene75194:CDS | 30.0% | |
| ! | AACTTTGAAGAATTTGAGGT+TGG | - | chr1.3:74366600-74366619 | MS.gene75194:CDS | 30.0% |
| ! | CAAATTGTGTTGTCATTTGA+TGG | - | chr1.3:74367586-74367605 | MS.gene75194:intron | 30.0% |
| ! | TCAACATGTAGTGAGTATTT+TGG | - | chr1.3:74366370-74366389 | MS.gene75194:intron | 30.0% |
| !! | TATTTGTCACTTTATCTAGC+TGG | - | chr1.3:74367471-74367490 | MS.gene75194:intron | 30.0% |
| !! | TTGTTTTGTACAAGGATGTT+TGG | - | chr1.3:74366410-74366429 | MS.gene75194:intron | 30.0% |
| !!! | GTAGCAATGTTTTAAAAACC+GGG | + | chr1.3:74366804-74366823 | None:intergenic | 30.0% |
| !!! | TTGATAATGTTTTGGGGTTT+AGG | - | chr1.3:74366453-74366472 | MS.gene75194:intron | 30.0% |
| AAAGCAGATTCGGATGTTTA+TGG | - | chr1.3:74367259-74367278 | MS.gene75194:CDS | 35.0% | |
| ACAATGTAGTCTGATTGAAC+TGG | + | chr1.3:74366751-74366770 | None:intergenic | 35.0% | |
| AGGATGAAACAAAGCAGATT+CGG | - | chr1.3:74367249-74367268 | MS.gene75194:CDS | 35.0% | |
| ATTATCTTCCATTTCAAGGC+TGG | + | chr1.3:74367641-74367660 | None:intergenic | 35.0% | |
| TATCAGAGCTGCATTTCTTT+AGG | - | chr1.3:74367432-74367451 | MS.gene75194:intron | 35.0% | |
| TGAGAATGGGAAGAATACTA+AGG | - | chr1.3:74366477-74366496 | MS.gene75194:intron | 35.0% | |
| ! | AAACAAGGGTTCAAGTTCTT+CGG | + | chr1.3:74366138-74366157 | None:intergenic | 35.0% |
| !! | CGGTTTAACTTGTTAAACCT+AGG | + | chr1.3:74366705-74366724 | None:intergenic | 35.0% |
| !!! | CTCCTTTTTCAATTCTCTGT+AGG | - | chr1.3:74367504-74367523 | MS.gene75194:intron | 35.0% |
| !!! | GGTTGTTTTTATTAGTGCTC+TGG | - | chr1.3:74367334-74367353 | MS.gene75194:intron | 35.0% |
| !!! | TTTTGCTGATTCAGCAAAAG+CGG | + | chr1.3:74367096-74367115 | None:intergenic | 35.0% |
| AACGCGACTGTAATCAAACA+AGG | + | chr1.3:74366153-74366172 | None:intergenic | 40.0% | |
| ACAAATCGGAAAACTGAACC+CGG | - | chr1.3:74366783-74366802 | MS.gene75194:intron | 40.0% | |
| ACGCGACTGTAATCAAACAA+GGG | + | chr1.3:74366152-74366171 | None:intergenic | 40.0% | |
| AGAATCTTTGCTCGTTCAGA+AGG | + | chr1.3:74367212-74367231 | None:intergenic | 40.0% | |
| CTCCTCATCAGTCTTCTTAT+GGG | + | chr1.3:74366575-74366594 | None:intergenic | 40.0% | |
| GAATGGGAAGAATACTAAGG+TGG | - | chr1.3:74366480-74366499 | MS.gene75194:intron | 40.0% | |
| GAGTTGAATCAGTTGAATCG+CGG | + | chr1.3:74366729-74366748 | None:intergenic | 40.0% | |
| TAAGGTGGAAACAGTGAATG+TGG | - | chr1.3:74366495-74366514 | MS.gene75194:intron | 40.0% | |
| TCTCATCTCTGTTCAAGACA+AGG | - | chr1.3:74367229-74367248 | MS.gene75194:CDS | 40.0% | |
| TGTAGGATGACCAGTTTGAA+AGG | - | chr1.3:74367521-74367540 | MS.gene75194:intron | 40.0% | |
| ! | TTTTTCACTTACGTCGTCCA+CGG | + | chr1.3:74367180-74367199 | None:intergenic | 40.0% |
| !! | TTGGGGTTTAGGTTGAGAAT+GGG | - | chr1.3:74366464-74366483 | MS.gene75194:intron | 40.0% |
| !! | TTTGGGGTTTAGGTTGAGAA+TGG | - | chr1.3:74366463-74366482 | MS.gene75194:intron | 40.0% |
| AGTCTTCTTATGGGCCTTAC+CGG | + | chr1.3:74366566-74366585 | None:intergenic | 45.0% | |
| CAATTCATTGCAACCTTCCG+TGG | - | chr1.3:74367160-74367179 | MS.gene75194:intron | 45.0% | |
| CACGGAAGGTTGCAATGAAT+TGG | + | chr1.3:74367162-74367181 | None:intergenic | 45.0% | |
| CCATAAGAAGACTGATGAGG+AGG | - | chr1.3:74366573-74366592 | MS.gene75194:CDS | 45.0% | |
| CCTCCTCATCAGTCTTCTTA+TGG | + | chr1.3:74366576-74366595 | None:intergenic | 45.0% | |
| GTAACCAATCATCATCCTCG+AGG | + | chr1.3:74366525-74366544 | None:intergenic | 45.0% | |
| TGAATCAGTTGAATCGCGGT+CGG | + | chr1.3:74366725-74366744 | None:intergenic | 45.0% | |
| !! | TTAAGGAGTGCTAACCATCG+AGG | + | chr1.3:74366193-74366212 | None:intergenic | 45.0% |
| !!! | TATTTTAGAATAAAAATAAA+AGG | + | chr1.3:74366272-74366291 | None:intergenic | 5.0% |
| ACCATCGAGGCACACGATAT+TGG | + | chr1.3:74366180-74366199 | None:intergenic | 50.0% | |
| AGGCAGGCAGATCAGAAACA+TGG | + | chr1.3:74367398-74367417 | None:intergenic | 50.0% | |
| ATTGGTTACCTTCGCCACCA+AGG | - | chr1.3:74366536-74366555 | MS.gene75194:intron | 50.0% | |
| CCATCGAGGCACACGATATT+GGG | + | chr1.3:74366179-74366198 | None:intergenic | 50.0% | |
| CTTCCATTTCAAGGCTGGCA+GGG | + | chr1.3:74367636-74367655 | None:intergenic | 50.0% | |
| GGCCCATAAGAAGACTGATG+AGG | - | chr1.3:74366570-74366589 | MS.gene75194:CDS | 50.0% | |
| GGTGGAAACAGTGAATGTGG+TGG | - | chr1.3:74366498-74366517 | MS.gene75194:intron | 50.0% | |
| TCTTCCATTTCAAGGCTGGC+AGG | + | chr1.3:74367637-74367656 | None:intergenic | 50.0% | |
| TGGACCTCGAGGATGATGAT+TGG | - | chr1.3:74366518-74366537 | MS.gene75194:intron | 50.0% | |
| TTCCATTTCAAGGCTGGCAG+GGG | + | chr1.3:74367635-74367654 | None:intergenic | 50.0% | |
| TTGGTTACCTTCGCCACCAA+GGG | - | chr1.3:74366537-74366556 | MS.gene75194:intron | 50.0% | |
| AACCCCTGCCAGCCTTGAAA+TGG | - | chr1.3:74367630-74367649 | MS.gene75194:CDS | 55.0% | |
| CCCAATATCGTGTGCCTCGA+TGG | - | chr1.3:74366176-74366195 | MS.gene75194:CDS | 55.0% | |
| TCACTTACGTCGTCCACGGA+AGG | + | chr1.3:74367176-74367195 | None:intergenic | 55.0% | |
| !! | AGTGAATGTGGTGGACCTCG+AGG | - | chr1.3:74366507-74366526 | MS.gene75194:intron | 55.0% |
| CCTTCGCCACCAAGGGTCAC+CGG | - | chr1.3:74366544-74366563 | MS.gene75194:CDS | 65.0% | |
| GCCACCAAGGGTCACCGGTA+AGG | - | chr1.3:74366549-74366568 | MS.gene75194:CDS | 65.0% | |
| GCCTTACCGGTGACCCTTGG+TGG | + | chr1.3:74366553-74366572 | None:intergenic | 65.0% | |
| TGGGCCTTACCGGTGACCCT+TGG | + | chr1.3:74366556-74366575 | None:intergenic | 65.0% | |
| !! | CCGGTGACCCTTGGTGGCGA+AGG | + | chr1.3:74366547-74366566 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 74366129 | 74367693 | 74366129 | ID=MS.gene75194 |
| chr1.3 | mRNA | 74366129 | 74367693 | 74366129 | ID=MS.gene75194.t1;Parent=MS.gene75194 |
| chr1.3 | exon | 74367625 | 74367693 | 74367625 | ID=MS.gene75194.t1.exon1;Parent=MS.gene75194.t1 |
| chr1.3 | CDS | 74367625 | 74367693 | 74367625 | ID=cds.MS.gene75194.t1;Parent=MS.gene75194.t1 |
| chr1.3 | exon | 74367205 | 74367347 | 74367205 | ID=MS.gene75194.t1.exon2;Parent=MS.gene75194.t1 |
| chr1.3 | CDS | 74367205 | 74367347 | 74367205 | ID=cds.MS.gene75194.t1;Parent=MS.gene75194.t1 |
| chr1.3 | exon | 74366542 | 74366749 | 74366542 | ID=MS.gene75194.t1.exon3;Parent=MS.gene75194.t1 |
| chr1.3 | CDS | 74366542 | 74366749 | 74366542 | ID=cds.MS.gene75194.t1;Parent=MS.gene75194.t1 |
| chr1.3 | exon | 74366129 | 74366296 | 74366129 | ID=MS.gene75194.t1.exon4;Parent=MS.gene75194.t1 |
| chr1.3 | CDS | 74366129 | 74366296 | 74366129 | ID=cds.MS.gene75194.t1;Parent=MS.gene75194.t1 |
| Gene Sequence |
| Protein sequence |
