AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058435


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058435.t1 MTR_1g014930 98.658 149 2 0 1 149 1 149 8.26e-108 303
MS.gene058435.t1 MTR_3g105340 54.839 124 45 4 13 135 11 124 2.44e-37 124
MS.gene058435.t1 MTR_8g021190 46.400 125 54 5 20 136 34 153 4.21e-30 107
MS.gene058435.t1 MTR_4g125030 52.727 110 44 3 6 113 15 118 5.45e-30 105
MS.gene058435.t1 MTR_4g125010 48.649 111 48 3 6 113 17 121 2.14e-28 102
MS.gene058435.t1 MTR_3g109860 37.374 99 58 2 13 110 33 128 1.63e-17 74.3
MS.gene058435.t1 MTR_1g103550 45.070 71 37 1 19 89 5 73 2.66e-17 73.2
MS.gene058435.t1 MTR_5g006430 39.560 91 49 2 17 107 3 87 3.55e-17 72.8
MS.gene058435.t1 MTR_8g013990 31.724 145 82 5 1 136 47 183 6.58e-16 71.2
MS.gene058435.t1 MTR_2g101140 45.455 77 31 2 17 86 63 135 1.76e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058435.t1 AT1G72510 43.939 132 57 5 19 136 36 164 7.97e-30 106
MS.gene058435.t1 AT1G72510 43.939 132 57 5 19 136 36 164 7.97e-30 106
MS.gene058435.t1 AT5G20670 43.478 138 63 3 11 136 17 151 1.26e-28 103
MS.gene058435.t1 AT2G09970 37.931 145 71 6 6 136 26 165 4.19e-24 92.0
MS.gene058435.t1 AT1G54095 44.037 109 59 2 1 109 1 107 1.63e-20 82.4
MS.gene058435.t1 AT3G22540 37.500 120 59 5 13 131 2 106 6.24e-19 77.0
MS.gene058435.t1 AT4G14819 36.134 119 59 4 13 131 2 103 1.21e-17 73.6
MS.gene058435.t1 AT2G25780 35.537 121 70 4 20 138 39 153 3.12e-14 66.2
MS.gene058435.t1 AT1G79770 70.000 40 12 0 17 56 52 91 1.15e-13 65.1
MS.gene058435.t1 AT5G25840 37.008 127 60 6 20 136 46 162 3.06e-13 63.9

Find 30 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAACTGCTGGCGAAATT+TGG 0.269881 1.4:-6951861 None:intergenic
CCGCAAAATGCAGCTGTTGC+GGG 0.402544 1.4:+6951678 MS.gene058435:CDS
CACGCAGAATGTTCCTTACT+CGG 0.438740 1.4:-6951968 None:intergenic
TTAGGACTTTGGATTCTCCT+AGG 0.457929 1.4:+6951939 MS.gene058435:CDS
TCCTAGGAGCAAGCCGAGTA+AGG 0.469773 1.4:+6951955 MS.gene058435:CDS
GGGAGAGATTCGGTGGAAGA+TGG 0.469879 1.4:+6951741 MS.gene058435:CDS
GTATGTGTCTCTGTCGTCCT+TGG 0.478341 1.4:-6952043 None:intergenic
ACCGCAAAATGCAGCTGTTG+CGG 0.482069 1.4:+6951677 MS.gene058435:CDS
CTGAGGCAGTGAAGGAAGAA+AGG 0.486207 1.4:+6951780 MS.gene058435:CDS
CATTCTGCGTGCAGACCTCT+TGG 0.504691 1.4:+6951980 MS.gene058435:CDS
AAGCAGAAGATCTGAACTGC+TGG 0.512505 1.4:-6951871 None:intergenic
GAAGGAAGAAAGGGCGAGGT+CGG 0.514158 1.4:+6951790 MS.gene058435:CDS
ATGCAGCTGTTGCGGGTTGG+TGG 0.517846 1.4:+6951685 MS.gene058435:CDS
AGAAGCTGAAGCGACAGAGA+TGG 0.523368 1.4:+6951643 MS.gene058435:CDS
TCCTTACTCGGCTTGCTCCT+AGG 0.526193 1.4:-6951956 None:intergenic
TGTGTCTCTGTCGTCCTTGG+CGG 0.528871 1.4:-6952040 None:intergenic
GCGCGCGTGAGGGAGAGATT+CGG 0.542153 1.4:+6951731 MS.gene058435:CDS
ACGCGTACATTGCGCGCGTG+AGG 0.548939 1.4:+6951720 MS.gene058435:CDS
CGCGTACATTGCGCGCGTGA+GGG 0.561079 1.4:+6951721 MS.gene058435:CDS
TCATAGTTGCTTCTCAACAA+TGG 0.575206 1.4:+6952009 MS.gene058435:CDS
TGAGGCAGTGAAGGAAGAAA+GGG 0.590842 1.4:+6951781 MS.gene058435:CDS
CAGTGAAGGAAGAAAGGGCG+AGG 0.593016 1.4:+6951786 MS.gene058435:CDS
TTTATGTGCTGAGGCAGTGA+AGG 0.603888 1.4:+6951772 MS.gene058435:CDS
CGCGTGAGGGAGAGATTCGG+TGG 0.607365 1.4:+6951734 MS.gene058435:CDS
AAAATGCAGCTGTTGCGGGT+TGG 0.633011 1.4:+6951682 MS.gene058435:CDS
CAATGGAAACAACTCCGCCA+AGG 0.641855 1.4:+6952026 MS.gene058435:CDS
TTCGGTGGAAGATGGATATG+CGG 0.649354 1.4:+6951749 MS.gene058435:CDS
CAACTATGAGATCTACCAAG+AGG 0.689504 1.4:-6951995 None:intergenic
CAATTGATGAAGCTTTAATG+CGG 0.691551 1.4:+6951834 MS.gene058435:CDS
GATATGCGGTTTATGTGCTG+AGG 0.715926 1.4:+6951763 MS.gene058435:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAATTGATGAAGCTTTAATG+CGG + chr1.4:6951834-6951853 MS.gene058435:CDS 30.0%
! CTGTGAAACAGATTCTTTTT+AGG + chr1.4:6951921-6951940 MS.gene058435:CDS 30.0%
!!! ACAGATTCTTTTTAGGACTT+TGG + chr1.4:6951928-6951947 MS.gene058435:CDS 30.0%
TCATAGTTGCTTCTCAACAA+TGG + chr1.4:6952009-6952028 MS.gene058435:CDS 35.0%
CAACTATGAGATCTACCAAG+AGG - chr1.4:6951998-6952017 None:intergenic 40.0%
TTAGGACTTTGGATTCTCCT+AGG + chr1.4:6951939-6951958 MS.gene058435:CDS 40.0%
AAGCAGAAGATCTGAACTGC+TGG - chr1.4:6951874-6951893 None:intergenic 45.0%
CACGCAGAATGTTCCTTACT+CGG - chr1.4:6951971-6951990 None:intergenic 45.0%
GATATGCGGTTTATGTGCTG+AGG + chr1.4:6951763-6951782 MS.gene058435:CDS 45.0%
TCTGAACTGCTGGCGAAATT+TGG - chr1.4:6951864-6951883 None:intergenic 45.0%
TGAGGCAGTGAAGGAAGAAA+GGG + chr1.4:6951781-6951800 MS.gene058435:CDS 45.0%
TTCGGTGGAAGATGGATATG+CGG + chr1.4:6951749-6951768 MS.gene058435:CDS 45.0%
TTTATGTGCTGAGGCAGTGA+AGG + chr1.4:6951772-6951791 MS.gene058435:CDS 45.0%
AAAATGCAGCTGTTGCGGGT+TGG + chr1.4:6951682-6951701 MS.gene058435:CDS 50.0%
ACCGCAAAATGCAGCTGTTG+CGG + chr1.4:6951677-6951696 MS.gene058435:CDS 50.0%
AGAAGCTGAAGCGACAGAGA+TGG + chr1.4:6951643-6951662 MS.gene058435:CDS 50.0%
CAATGGAAACAACTCCGCCA+AGG + chr1.4:6952026-6952045 MS.gene058435:CDS 50.0%
CTGAGGCAGTGAAGGAAGAA+AGG + chr1.4:6951780-6951799 MS.gene058435:CDS 50.0%
GTATGTGTCTCTGTCGTCCT+TGG - chr1.4:6952046-6952065 None:intergenic 50.0%
CAGTGAAGGAAGAAAGGGCG+AGG + chr1.4:6951786-6951805 MS.gene058435:CDS 55.0%
CATTCTGCGTGCAGACCTCT+TGG + chr1.4:6951980-6951999 MS.gene058435:CDS 55.0%
CCGCAAAATGCAGCTGTTGC+GGG + chr1.4:6951678-6951697 MS.gene058435:CDS 55.0%
GAAGGAAGAAAGGGCGAGGT+CGG + chr1.4:6951790-6951809 MS.gene058435:CDS 55.0%
TCCTAGGAGCAAGCCGAGTA+AGG + chr1.4:6951955-6951974 MS.gene058435:CDS 55.0%
TGTGTCTCTGTCGTCCTTGG+CGG - chr1.4:6952043-6952062 None:intergenic 55.0%
! CCCGCAACAGCTGCATTTTG+CGG - chr1.4:6951681-6951700 None:intergenic 55.0%
! TCCTTACTCGGCTTGCTCCT+AGG - chr1.4:6951959-6951978 None:intergenic 55.0%
!! GGGAGAGATTCGGTGGAAGA+TGG + chr1.4:6951741-6951760 MS.gene058435:CDS 55.0%
!! ATGCAGCTGTTGCGGGTTGG+TGG + chr1.4:6951685-6951704 MS.gene058435:CDS 60.0%
ACGCGTACATTGCGCGCGTG+AGG + chr1.4:6951720-6951739 MS.gene058435:CDS 65.0%
CGCGTACATTGCGCGCGTGA+GGG + chr1.4:6951721-6951740 MS.gene058435:CDS 65.0%
CGCGTGAGGGAGAGATTCGG+TGG + chr1.4:6951734-6951753 MS.gene058435:CDS 65.0%
GCGCGCGTGAGGGAGAGATT+CGG + chr1.4:6951731-6951750 MS.gene058435:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 6951623 6952072 6951623 ID=MS.gene058435
chr1.4 mRNA 6951623 6952072 6951623 ID=MS.gene058435.t1;Parent=MS.gene058435
chr1.4 exon 6951623 6952072 6951623 ID=MS.gene058435.t1.exon1;Parent=MS.gene058435.t1
chr1.4 CDS 6951623 6952072 6951623 ID=cds.MS.gene058435.t1;Parent=MS.gene058435.t1
Gene Sequence

>MS.gene058435

ATGGCTGCGAATGTTGTGAAAGAAGCTGAAGCGACAGAGATGGAAGCTGTTTTGACCGCAAAATGCAGCTGTTGCGGGTTGGTGGAAGAGTGCACACACGCGTACATTGCGCGCGTGAGGGAGAGATTCGGTGGAAGATGGATATGCGGTTTATGTGCTGAGGCAGTGAAGGAAGAAAGGGCGAGGTCGGAGAGTGATGAGAAGATGATCACAATTGATGAAGCTTTAATGCGGCACACCAAATTTCGCCAGCAGTTCAGATCTTCTGCTTCTGATCATAACAAAGATTTCATAGTTGCTGTGAAACAGATTCTTTTTAGGACTTTGGATTCTCCTAGGAGCAAGCCGAGTAAGGAACATTCTGCGTGCAGACCTCTTGGTAGATCTCATAGTTGCTTCTCAACAATGGAAACAACTCCGCCAAGGACGACAGAGACACATACAGAGTGA

Protein sequence

>MS.gene058435.t1

MAANVVKEAEATEMEAVLTAKCSCCGLVEECTHAYIARVRERFGGRWICGLCAEAVKEERARSESDEKMITIDEALMRHTKFRQQFRSSASDHNKDFIVAVKQILFRTLDSPRSKPSKEHSACRPLGRSHSCFSTMETTPPRTTETHTE