Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75208.t1 | RHN82399.1 | 93.9 | 198 | 12 | 0 | 1 | 198 | 50 | 247 | 7.00E-103 | 383.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75208.t1 | G7IC90 | 93.9 | 198 | 12 | 0 | 1 | 198 | 50 | 247 | 5.1e-103 | 383.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052493 | MS.gene75208 | 0.800267 | 1.56E-48 | -1.69E-46 |
| MS.gene052674 | MS.gene75208 | 0.80204 | 6.77E-49 | -1.69E-46 |
| MS.gene052675 | MS.gene75208 | 0.808165 | 3.54E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene75208 | 0.806463 | 8.13E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene75208 | 0.80116 | 1.03E-48 | -1.69E-46 |
| MS.gene057155 | MS.gene75208 | 0.814468 | 1.52E-51 | -1.69E-46 |
| MS.gene060520 | MS.gene75208 | 0.822361 | 2.47E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75208.t1 | MTR_1g110270 | 93.939 | 198 | 12 | 0 | 1 | 198 | 50 | 247 | 3.72e-132 | 385 |
| MS.gene75208.t1 | MTR_1g110120 | 28.788 | 198 | 121 | 5 | 2 | 196 | 58 | 238 | 7.29e-15 | 72.8 |
| MS.gene75208.t1 | MTR_1g108890 | 24.880 | 209 | 109 | 7 | 1 | 196 | 61 | 234 | 4.83e-14 | 68.9 |
| MS.gene75208.t1 | MTR_1g110220 | 27.751 | 209 | 117 | 8 | 1 | 196 | 45 | 232 | 1.26e-13 | 68.9 |
| MS.gene75208.t1 | MTR_1g110110 | 27.273 | 209 | 118 | 8 | 1 | 196 | 45 | 232 | 4.40e-13 | 67.4 |
| MS.gene75208.t1 | MTR_1g110130 | 27.228 | 202 | 125 | 5 | 1 | 198 | 51 | 234 | 3.76e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATTCTAATCATCTTAA+AGG | 0.110803 | 1.4:-55358130 | MS.gene75208:CDS |
| TCTAGCTGCCATGATCTTTA+TGG | 0.182708 | 1.4:-55357992 | MS.gene75208:CDS |
| TACTACTTTGTATGGTGTTT+CGG | 0.278631 | 1.4:-55358203 | MS.gene75208:CDS |
| GCCTTTGGTTTCATCTCGTT+TGG | 0.303933 | 1.4:-55358326 | MS.gene75208:CDS |
| TACCTTGTTTACAATAAAAC+TGG | 0.311088 | 1.4:+55357942 | None:intergenic |
| TCCTTTAAGATGATTAGAAT+TGG | 0.355034 | 1.4:+55358129 | None:intergenic |
| TACCTATGATTCCATGTTCA+CGG | 0.399707 | 1.4:-55358356 | MS.gene75208:CDS |
| GAAGATACTACTACTTTGTA+TGG | 0.411932 | 1.4:-55358211 | MS.gene75208:CDS |
| AGTAGTATCTTCTCTACAAA+TGG | 0.419233 | 1.4:+55358222 | None:intergenic |
| TCTTTGATTGTGATTGTGAA+TGG | 0.428531 | 1.4:+55358424 | None:intergenic |
| GTTCACGGTCAACACGCCTT+TGG | 0.432660 | 1.4:-55358341 | MS.gene75208:CDS |
| GACCGTGAACATGGAATCAT+AGG | 0.463207 | 1.4:+55358354 | None:intergenic |
| AAAGAGTAATAAAGCTATTG+TGG | 0.490569 | 1.4:-55358170 | MS.gene75208:CDS |
| CGGTCAACCTTTGAAATCGT+TGG | 0.507527 | 1.4:+55358088 | None:intergenic |
| AGGCGTGTTGACCGTGAACA+TGG | 0.520595 | 1.4:+55358345 | None:intergenic |
| GTAGCAAAAGATCGTGGAGT+TGG | 0.529694 | 1.4:+55358398 | None:intergenic |
| CCATACAAAACAACAAAAGA+TGG | 0.531064 | 1.4:-55358468 | MS.gene75208:CDS |
| AGCTGCCATGATCTTTATGG+TGG | 0.557488 | 1.4:-55357989 | MS.gene75208:CDS |
| ATTGACCACCATAAAGATCA+TGG | 0.567413 | 1.4:+55357984 | None:intergenic |
| TGAGCTTCCAACGATTTCAA+AGG | 0.575738 | 1.4:-55358095 | MS.gene75208:CDS |
| CAATGTCTACTTGATAACAT+TGG | 0.613597 | 1.4:-55357965 | MS.gene75208:CDS |
| TGTGGCATGTTAGTAATACA+TGG | 0.621263 | 1.4:-55358523 | None:intergenic |
| AAAGATCAGCAGGATCAAGA+CGG | 0.654516 | 1.4:+55358068 | None:intergenic |
| TCCAAACGAGATGAAACCAA+AGG | 0.670240 | 1.4:+55358325 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAGTAATAAAGCTATTG+TGG | - | chr1.4:55358292-55358311 | MS.gene75208:CDS | 25.0% |
| ! | TCCAATTCTAATCATCTTAA+AGG | - | chr1.4:55358332-55358351 | MS.gene75208:CDS | 25.0% |
| !! | GAATATTACGTAACTGTTTT+CGG | - | chr1.4:55358158-55358177 | MS.gene75208:CDS | 25.0% |
| !!! | GTTGTTTTGTATGGTTTTTT+TGG | + | chr1.4:55357988-55358007 | None:intergenic | 25.0% |
| !!! | TCCTTTAAGATGATTAGAAT+TGG | + | chr1.4:55358336-55358355 | None:intergenic | 25.0% |
| AGTAGTATCTTCTCTACAAA+TGG | + | chr1.4:55358243-55358262 | None:intergenic | 30.0% | |
| CCATACAAAACAACAAAAGA+TGG | - | chr1.4:55357994-55358013 | MS.gene75208:CDS | 30.0% | |
| GAAGATACTACTACTTTGTA+TGG | - | chr1.4:55358251-55358270 | MS.gene75208:CDS | 30.0% | |
| TACTACTTTGTATGGTGTTT+CGG | - | chr1.4:55358259-55358278 | MS.gene75208:CDS | 30.0% | |
| TCTTTGATTGTGATTGTGAA+TGG | + | chr1.4:55358041-55358060 | None:intergenic | 30.0% | |
| ! | CAATGTCTACTTGATAACAT+TGG | - | chr1.4:55358497-55358516 | MS.gene75208:CDS | 30.0% |
| ! | GATCTTTTTAACGTGTTAAG+CGG | - | chr1.4:55358410-55358429 | MS.gene75208:CDS | 30.0% |
| !!! | CCATCTTTTGTTGTTTTGTA+TGG | + | chr1.4:55357997-55358016 | None:intergenic | 30.0% |
| !!! | TGTATGGTTTTTTTGGATTG+TGG | + | chr1.4:55357981-55358000 | None:intergenic | 30.0% |
| !!! | TTTTTTGTAGCAAAAGATCG+TGG | + | chr1.4:55358073-55358092 | None:intergenic | 30.0% |
| AACACGTTAAAAAGATCAGC+AGG | + | chr1.4:55358407-55358426 | None:intergenic | 35.0% | |
| ATTGACCACCATAAAGATCA+TGG | + | chr1.4:55358481-55358500 | None:intergenic | 35.0% | |
| TACCTATGATTCCATGTTCA+CGG | - | chr1.4:55358106-55358125 | MS.gene75208:CDS | 35.0% | |
| AAAGATCAGCAGGATCAAGA+CGG | + | chr1.4:55358397-55358416 | None:intergenic | 40.0% | |
| TCCAAACGAGATGAAACCAA+AGG | + | chr1.4:55358140-55358159 | None:intergenic | 40.0% | |
| TCTAGCTGCCATGATCTTTA+TGG | - | chr1.4:55358470-55358489 | MS.gene75208:CDS | 40.0% | |
| TGAGCTTCCAACGATTTCAA+AGG | - | chr1.4:55358367-55358386 | MS.gene75208:CDS | 40.0% | |
| AGCTGCCATGATCTTTATGG+TGG | - | chr1.4:55358473-55358492 | MS.gene75208:CDS | 45.0% | |
| GACCGTGAACATGGAATCAT+AGG | + | chr1.4:55358111-55358130 | None:intergenic | 45.0% | |
| GTAGCAAAAGATCGTGGAGT+TGG | + | chr1.4:55358067-55358086 | None:intergenic | 45.0% | |
| ! | GCCTTTGGTTTCATCTCGTT+TGG | - | chr1.4:55358136-55358155 | MS.gene75208:CDS | 45.0% |
| !! | CGGTCAACCTTTGAAATCGT+TGG | + | chr1.4:55358377-55358396 | None:intergenic | 45.0% |
| GTTCACGGTCAACACGCCTT+TGG | - | chr1.4:55358121-55358140 | MS.gene75208:CDS | 55.0% | |
| ! | AGGCGTGTTGACCGTGAACA+TGG | + | chr1.4:55358120-55358139 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 55357945 | 55358539 | 55357945 | ID=MS.gene75208 |
| chr1.4 | mRNA | 55357945 | 55358539 | 55357945 | ID=MS.gene75208.t1;Parent=MS.gene75208 |
| chr1.4 | exon | 55357945 | 55358539 | 55357945 | ID=MS.gene75208.t1.exon1;Parent=MS.gene75208.t1 |
| chr1.4 | CDS | 55357945 | 55358539 | 55357945 | ID=cds.MS.gene75208.t1;Parent=MS.gene75208.t1 |
| Gene Sequence |
| Protein sequence |
