Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75382.t1 | XP_024638013.1 | 93.5 | 108 | 5 | 1 | 1 | 108 | 1 | 106 | 1.90E-46 | 194.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75382.t1 | Q9SSC1 | 43.3 | 141 | 44 | 7 | 1 | 108 | 1 | 138 | 5.7e-15 | 81.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75382.t1 | A0A396HYZ6 | 93.5 | 108 | 5 | 1 | 1 | 108 | 1 | 106 | 1.4e-46 | 194.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75382 | MS.gene86369 | PPI |
| MS.gene060879 | MS.gene75382 | PPI |
| MS.gene36231 | MS.gene75382 | PPI |
| MS.gene36229 | MS.gene75382 | PPI |
| MS.gene89049 | MS.gene75382 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75382.t1 | MTR_4g011060 | 93.458 | 107 | 5 | 1 | 1 | 107 | 1 | 105 | 6.92e-67 | 197 |
| MS.gene75382.t1 | MTR_2g103380 | 58.475 | 118 | 39 | 1 | 1 | 108 | 1 | 118 | 1.45e-40 | 130 |
| MS.gene75382.t1 | MTR_4g114050 | 53.125 | 96 | 31 | 4 | 1 | 95 | 1 | 83 | 8.23e-24 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75382.t1 | AT1G80180 | 43.262 | 141 | 44 | 7 | 1 | 108 | 1 | 138 | 1.79e-23 | 88.2 |
| MS.gene75382.t1 | AT5G20100 | 49.495 | 99 | 46 | 3 | 3 | 98 | 2 | 99 | 6.12e-21 | 80.9 |
| MS.gene75382.t1 | AT1G15400 | 40.909 | 132 | 46 | 7 | 1 | 105 | 1 | 127 | 8.58e-20 | 78.6 |
| MS.gene75382.t1 | AT1G15400 | 40.909 | 132 | 46 | 7 | 1 | 105 | 1 | 127 | 9.75e-20 | 79.0 |
| MS.gene75382.t1 | AT1G15400 | 40.909 | 132 | 46 | 7 | 1 | 105 | 1 | 127 | 9.90e-20 | 78.6 |
Find 29 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTCATCTGGTCTTGTT+TGG | 0.224185 | 4.2:+89290348 | MS.gene75382:CDS |
| TGTGGATTCTGTACTCCTTT+TGG | 0.270248 | 4.2:+89290566 | MS.gene75382:CDS |
| AGGTTCATCTGGTCTTGTTT+GGG | 0.315565 | 4.2:+89290349 | MS.gene75382:CDS |
| CTACCTCGACCGACGTTTAC+CGG | 0.339840 | 4.2:-89290612 | None:intergenic |
| TGGGATGACAGATTCTTATC+AGG | 0.348639 | 4.2:+89290368 | MS.gene75382:CDS |
| CTGAATGATACTTCAGATCT+TGG | 0.351403 | 4.2:-89290320 | None:intergenic |
| AGAAGCCGCTCCACCGGTGC+TGG | 0.377778 | 4.2:+89290479 | MS.gene75382:CDS |
| CGCTCCACCGGTGCTGGAAG+AGG | 0.415568 | 4.2:+89290485 | MS.gene75382:CDS |
| TTCAGAAGATCAGGTTCATC+TGG | 0.432719 | 4.2:+89290338 | MS.gene75382:CDS |
| GAAGTATCATTCAGAAGATC+AGG | 0.445781 | 4.2:+89290329 | MS.gene75382:CDS |
| GGTGGAGCGGCTTCTTTGTA+CGG | 0.474965 | 4.2:-89290471 | None:intergenic |
| TACCTCGACCGACGTTTACC+GGG | 0.526089 | 4.2:-89290611 | None:intergenic |
| AATCCACACGCAGAGACCTT+CGG | 0.527997 | 4.2:-89290551 | None:intergenic |
| GGAGACGGTGGTTCGATCGC+CGG | 0.536833 | 4.2:-89290530 | None:intergenic |
| TCTCCGAAGGTCTCTGCGTG+TGG | 0.540911 | 4.2:+89290548 | MS.gene75382:CDS |
| CTCTTCCAGCACCGGTGGAG+CGG | 0.560632 | 4.2:-89290484 | None:intergenic |
| GCTGGAAGAGGATACCGTAC+TGG | 0.563508 | 4.2:+89290497 | MS.gene75382:CDS |
| GTACAAAGAAGCCGCTCCAC+CGG | 0.567417 | 4.2:+89290473 | MS.gene75382:CDS |
| AGCCCGGTAAACGTCGGTCG+AGG | 0.570758 | 4.2:+89290609 | MS.gene75382:CDS |
| ACGGTATCCTCTTCCAGCAC+CGG | 0.571388 | 4.2:-89290492 | None:intergenic |
| CACGCAGAGACCTTCGGAGA+CGG | 0.586598 | 4.2:-89290545 | None:intergenic |
| GATCGAACCACCGTCTCCGA+AGG | 0.590024 | 4.2:+89290535 | MS.gene75382:CDS |
| GTATCCTCTTCCAGCACCGG+TGG | 0.591894 | 4.2:-89290489 | None:intergenic |
| AAGGGTCAACGATCAAAGCC+CGG | 0.598386 | 4.2:+89290593 | MS.gene75382:CDS |
| CCGTACTGGAAAAGTTTCAC+CGG | 0.598922 | 4.2:+89290511 | MS.gene75382:CDS |
| CGGTAAACGTCGGTCGAGGT+AGG | 0.609038 | 4.2:+89290613 | MS.gene75382:CDS |
| GGAGCGGCTTCTTTGTACGG+TGG | 0.640187 | 4.2:-89290468 | None:intergenic |
| GCAGAGACCTTCGGAGACGG+TGG | 0.676367 | 4.2:-89290542 | None:intergenic |
| GATCAAAGCCCGGTAAACGT+CGG | 0.721418 | 4.2:+89290603 | MS.gene75382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGAATGATACTTCAGATCT+TGG | - | chr4.2:89290323-89290342 | None:intergenic | 35.0% | |
| ! | GAAGTATCATTCAGAAGATC+AGG | + | chr4.2:89290329-89290348 | MS.gene75382:CDS | 35.0% |
| !! | TGTACTCCTTTTGGGAAAAA+GGG | + | chr4.2:89290575-89290594 | MS.gene75382:CDS | 35.0% |
| TGGGATGACAGATTCTTATC+AGG | + | chr4.2:89290368-89290387 | MS.gene75382:CDS | 40.0% | |
| TTCAGAAGATCAGGTTCATC+TGG | + | chr4.2:89290338-89290357 | MS.gene75382:CDS | 40.0% | |
| ! | GGTTTTCACATGAAGCTCTT+TGG | - | chr4.2:89290450-89290469 | None:intergenic | 40.0% |
| ! | GTGGATTCTGTACTCCTTTT+GGG | + | chr4.2:89290567-89290586 | MS.gene75382:CDS | 40.0% |
| ! | TGTGGATTCTGTACTCCTTT+TGG | + | chr4.2:89290566-89290585 | MS.gene75382:CDS | 40.0% |
| !! | AGGTTCATCTGGTCTTGTTT+GGG | + | chr4.2:89290349-89290368 | MS.gene75382:CDS | 40.0% |
| !! | CTGTACTCCTTTTGGGAAAA+AGG | + | chr4.2:89290574-89290593 | MS.gene75382:CDS | 40.0% |
| CCGTACTGGAAAAGTTTCAC+CGG | + | chr4.2:89290511-89290530 | MS.gene75382:CDS | 45.0% | |
| ! | CCGGTGAAACTTTTCCAGTA+CGG | - | chr4.2:89290514-89290533 | None:intergenic | 45.0% |
| ! | CGTTGACCCTTTTTCCCAAA+AGG | - | chr4.2:89290584-89290603 | None:intergenic | 45.0% |
| !! | CAGGTTCATCTGGTCTTGTT+TGG | + | chr4.2:89290348-89290367 | MS.gene75382:CDS | 45.0% |
| AAGGGTCAACGATCAAAGCC+CGG | + | chr4.2:89290593-89290612 | MS.gene75382:CDS | 50.0% | |
| AATCCACACGCAGAGACCTT+CGG | - | chr4.2:89290554-89290573 | None:intergenic | 50.0% | |
| GATCAAAGCCCGGTAAACGT+CGG | + | chr4.2:89290603-89290622 | MS.gene75382:CDS | 50.0% | |
| ACGGTATCCTCTTCCAGCAC+CGG | - | chr4.2:89290495-89290514 | None:intergenic | 55.0% | |
| CTACCTCGACCGACGTTTAC+CGG | - | chr4.2:89290615-89290634 | None:intergenic | 55.0% | |
| GCTGGAAGAGGATACCGTAC+TGG | + | chr4.2:89290497-89290516 | MS.gene75382:CDS | 55.0% | |
| GGTGGAGCGGCTTCTTTGTA+CGG | - | chr4.2:89290474-89290493 | None:intergenic | 55.0% | |
| GTACAAAGAAGCCGCTCCAC+CGG | + | chr4.2:89290473-89290492 | MS.gene75382:CDS | 55.0% | |
| TACCTCGACCGACGTTTACC+GGG | - | chr4.2:89290614-89290633 | None:intergenic | 55.0% | |
| CACGCAGAGACCTTCGGAGA+CGG | - | chr4.2:89290548-89290567 | None:intergenic | 60.0% | |
| GATCGAACCACCGTCTCCGA+AGG | + | chr4.2:89290535-89290554 | MS.gene75382:CDS | 60.0% | |
| GGAGCGGCTTCTTTGTACGG+TGG | - | chr4.2:89290471-89290490 | None:intergenic | 60.0% | |
| TCTCCGAAGGTCTCTGCGTG+TGG | + | chr4.2:89290548-89290567 | MS.gene75382:CDS | 60.0% | |
| ! | GTATCCTCTTCCAGCACCGG+TGG | - | chr4.2:89290492-89290511 | None:intergenic | 60.0% |
| AGCCCGGTAAACGTCGGTCG+AGG | + | chr4.2:89290609-89290628 | MS.gene75382:CDS | 65.0% | |
| GCAGAGACCTTCGGAGACGG+TGG | - | chr4.2:89290545-89290564 | None:intergenic | 65.0% | |
| GGAGACGGTGGTTCGATCGC+CGG | - | chr4.2:89290533-89290552 | None:intergenic | 65.0% | |
| !! | CTCTTCCAGCACCGGTGGAG+CGG | - | chr4.2:89290487-89290506 | None:intergenic | 65.0% |
| AGAAGCCGCTCCACCGGTGC+TGG | + | chr4.2:89290479-89290498 | MS.gene75382:CDS | 70.0% | |
| ! | CGCTCCACCGGTGCTGGAAG+AGG | + | chr4.2:89290485-89290504 | MS.gene75382:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 89290308 | 89290634 | 89290308 | ID=MS.gene75382 |
| chr4.2 | mRNA | 89290308 | 89290634 | 89290308 | ID=MS.gene75382.t1;Parent=MS.gene75382 |
| chr4.2 | exon | 89290308 | 89290634 | 89290308 | ID=MS.gene75382.t1.exon1;Parent=MS.gene75382.t1 |
| chr4.2 | CDS | 89290308 | 89290634 | 89290308 | ID=cds.MS.gene75382.t1;Parent=MS.gene75382.t1 |
| Gene Sequence |
| Protein sequence |
