Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75631.t1 | XP_003601750.1 | 97.7 | 131 | 2 | 1 | 1 | 131 | 1 | 130 | 4.30E-56 | 227.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75631.t1 | G7JBE6 | 97.7 | 131 | 2 | 1 | 1 | 131 | 1 | 130 | 3.1e-56 | 227.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050380 | MS.gene75631 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene053562 | MS.gene75631 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene055202 | MS.gene75631 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene05601 | MS.gene75631 | -0.80687 | 6.67E-50 | -1.69E-46 |
| MS.gene057173 | MS.gene75631 | 0.830731 | 2.49E-55 | -1.69E-46 |
| MS.gene057473 | MS.gene75631 | 0.808345 | 3.24E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene75631 | -0.82408 | 9.80E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene75631 | -0.804184 | 2.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75631.t1 | MTR_3g084990 | 98.473 | 131 | 1 | 1 | 1 | 131 | 1 | 130 | 4.41e-89 | 254 |
| MS.gene75631.t1 | MTR_8g097040 | 54.348 | 138 | 48 | 5 | 2 | 131 | 1 | 131 | 6.49e-42 | 135 |
| MS.gene75631.t1 | MTR_5g078400 | 88.235 | 68 | 8 | 0 | 15 | 82 | 4 | 71 | 8.39e-37 | 120 |
| MS.gene75631.t1 | MTR_7g028805 | 37.500 | 128 | 61 | 3 | 1 | 109 | 1 | 128 | 6.34e-17 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75631.t1 | AT1G27290 | 56.944 | 144 | 47 | 2 | 1 | 131 | 1 | 142 | 1.29e-50 | 157 |
| MS.gene75631.t1 | AT1G27290 | 56.944 | 144 | 47 | 2 | 1 | 131 | 1 | 142 | 1.29e-50 | 157 |
Find 38 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCGGTCACGTTCTTTAT+TGG | 0.225023 | 3.3:+71028570 | MS.gene75631:CDS |
| GTGGGTTATTGGATTGATTA+TGG | 0.317283 | 3.3:+71028608 | MS.gene75631:CDS |
| ATTATGGTTCTCTATGTTGC+TGG | 0.354926 | 3.3:+71028624 | MS.gene75631:CDS |
| CCGCGTAAAGATTCTATTCC+AGG | 0.361280 | 3.3:+71028717 | MS.gene75631:CDS |
| TCTTTACGCGGCGGTGGCTC+CGG | 0.404881 | 3.3:-71028705 | None:intergenic |
| CGGTTGCTCCGGTGGATGAT+TGG | 0.416801 | 3.3:-71028463 | None:intergenic |
| GTTACCACTCTCTCTGATAT+AGG | 0.444657 | 3.3:+71028651 | MS.gene75631:CDS |
| CGGCGGTGGCTCCGGCGCCG+CGG | 0.448343 | 3.3:-71028697 | None:intergenic |
| GAGAAATTTAACGGCGTTGG+TGG | 0.453775 | 3.3:-71028441 | None:intergenic |
| CGCCGTAACGGTTGCTCCGG+TGG | 0.458989 | 3.3:-71028471 | None:intergenic |
| AGCAGAGAGGCAAAACCCGC+AGG | 0.469362 | 3.3:-71028516 | None:intergenic |
| GGTTGAGAAGGATTGCAGAT+AGG | 0.472984 | 3.3:-71028407 | None:intergenic |
| GGATGATTGGAGAAATTTAA+CGG | 0.478874 | 3.3:-71028450 | None:intergenic |
| TCTGCTTCTGGGAATTTCGA+TGG | 0.499850 | 3.3:+71028533 | MS.gene75631:CDS |
| CTGTTATATCACCGCGGCGC+CGG | 0.499951 | 3.3:+71028686 | MS.gene75631:CDS |
| TTGGAGAAATTTAACGGCGT+TGG | 0.502757 | 3.3:-71028444 | None:intergenic |
| ATAGAATCTTTACGCGGCGG+TGG | 0.505827 | 3.3:-71028711 | None:intergenic |
| GGCTCTGATGCTCTGTGGAT+CGG | 0.521250 | 3.3:+71028554 | MS.gene75631:CDS |
| TTAACGGCGTTGGTGGTGAA+CGG | 0.531777 | 3.3:-71028434 | None:intergenic |
| GTGAACGGAGGAGGTTGAGA+AGG | 0.548099 | 3.3:-71028419 | None:intergenic |
| GCGTTGGTGGTGAACGGAGG+AGG | 0.549273 | 3.3:-71028428 | None:intergenic |
| CCACCTCAAATCTCACCTGC+GGG | 0.549426 | 3.3:+71028501 | MS.gene75631:CDS |
| AAACGCCGTAACGGTTGCTC+CGG | 0.554974 | 3.3:-71028474 | None:intergenic |
| AAACCCGCAGGTGAGATTTG+AGG | 0.566480 | 3.3:-71028504 | None:intergenic |
| ATCCACCGGAGCAACCGTTA+CGG | 0.568881 | 3.3:+71028469 | MS.gene75631:CDS |
| AAATTTCTCCAATCATCCAC+CGG | 0.571678 | 3.3:+71028455 | MS.gene75631:CDS |
| GGTGGAGGTAAACGCCGTAA+CGG | 0.594940 | 3.3:-71028483 | None:intergenic |
| GCAGGTGAGATTTGAGGTGG+AGG | 0.603112 | 3.3:-71028498 | None:intergenic |
| CCCGCAGGTGAGATTTGAGG+TGG | 0.619368 | 3.3:-71028501 | None:intergenic |
| TCCACCTCAAATCTCACCTG+CGG | 0.630396 | 3.3:+71028500 | MS.gene75631:CDS |
| ACGACCTATATCAGAGAGAG+TGG | 0.640005 | 3.3:-71028655 | None:intergenic |
| TCGATGGCTCTGATGCTCTG+TGG | 0.642765 | 3.3:+71028549 | MS.gene75631:CDS |
| ACGGCGTTGGTGGTGAACGG+AGG | 0.653070 | 3.3:-71028431 | None:intergenic |
| ATAATCAATCCAATAACCCA+CGG | 0.673735 | 3.3:-71028606 | None:intergenic |
| CGAAATTCCCAGAAGCAGAG+AGG | 0.678634 | 3.3:-71028529 | None:intergenic |
| CCTGGAATAGAATCTTTACG+CGG | 0.704020 | 3.3:-71028717 | None:intergenic |
| GGAATAGAATCTTTACGCGG+CGG | 0.733807 | 3.3:-71028714 | None:intergenic |
| TGTTCTCTGTTATATCACCG+CGG | 0.748178 | 3.3:+71028680 | MS.gene75631:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTATATACATAATGTATTA+AGG | - | chr3.3:71029223-71029242 | None:intergenic | 15.0% |
| !!! | TCAATGATTTTATTTAACAA+AGG | + | chr3.3:71028838-71028857 | MS.gene75631:intron | 15.0% |
| !! | AATGCATACATATATCTTAA+TGG | - | chr3.3:71029184-71029203 | None:intergenic | 20.0% |
| !! | TAGTAATGAACTAAATGTTT+AGG | + | chr3.3:71029157-71029176 | MS.gene75631:intron | 20.0% |
| !!! | AAAATTTTTGTTATCTTACC+TGG | - | chr3.3:71028738-71028757 | None:intergenic | 20.0% |
| !!! | TACATAATGTATTAAGGTTT+TGG | - | chr3.3:71029217-71029236 | None:intergenic | 20.0% |
| !!! | TTCAAATTTTCCAAAGAAAT+GGG | + | chr3.3:71028965-71028984 | MS.gene75631:intron | 20.0% |
| !!! | TTTCAAATTTTCCAAAGAAA+TGG | + | chr3.3:71028964-71028983 | MS.gene75631:intron | 20.0% |
| ! | AAACAAGTATGAGACAAAAA+AGG | - | chr3.3:71028813-71028832 | None:intergenic | 25.0% |
| ! | ACTACTCAAAATTTGATGTT+TGG | + | chr3.3:71029322-71029341 | MS.gene75631:intron | 25.0% |
| !! | TTTCTTTCTTTTTCTGTGTT+GGG | + | chr3.3:71028917-71028936 | MS.gene75631:intron | 25.0% |
| !! | TTTTCTTTCTTTTTCTGTGT+TGG | + | chr3.3:71028916-71028935 | MS.gene75631:intron | 25.0% |
| !!! | CTAGGACAACATTTTTTTAA+GGG | - | chr3.3:71029358-71029377 | None:intergenic | 25.0% |
| !!! | TTTGTCTTTTGTTTCGTATT+TGG | + | chr3.3:71028778-71028797 | MS.gene75631:intron | 25.0% |
| AGCGAAAATCGAACATATTT+GGG | - | chr3.3:71029019-71029038 | None:intergenic | 30.0% | |
| ATAATCAATCCAATAACCCA+CGG | - | chr3.3:71028609-71028628 | None:intergenic | 30.0% | |
| ATTAAGCCACACATGATAAA+AGG | - | chr3.3:71029402-71029421 | None:intergenic | 30.0% | |
| GGATGATTGGAGAAATTTAA+CGG | - | chr3.3:71028453-71028472 | None:intergenic | 30.0% | |
| TGGTTTGATCATAAAAGACA+AGG | + | chr3.3:71029112-71029131 | MS.gene75631:intron | 30.0% | |
| TTAAGCCACACATGATAAAA+GGG | - | chr3.3:71029401-71029420 | None:intergenic | 30.0% | |
| ! | GGATGCAAATTGATATGTTT+TGG | + | chr3.3:71028986-71029005 | MS.gene75631:intron | 30.0% |
| !!! | GCTAGGACAACATTTTTTTA+AGG | - | chr3.3:71029359-71029378 | None:intergenic | 30.0% |
| AAAAAATGTTGTCCTAGCTG+CGG | + | chr3.3:71029361-71029380 | MS.gene75631:intron | 35.0% | |
| AAATTTCTCCAATCATCCAC+CGG | + | chr3.3:71028455-71028474 | MS.gene75631:CDS | 35.0% | |
| AAGGTGTATGTGAATTTCGT+GGG | + | chr3.3:71029131-71029150 | MS.gene75631:intron | 35.0% | |
| ATTATGGTTCTCTATGTTGC+TGG | + | chr3.3:71028624-71028643 | MS.gene75631:CDS | 35.0% | |
| CAATTTGCATCCCATTTCTT+TGG | - | chr3.3:71028978-71028997 | None:intergenic | 35.0% | |
| CAGCGAAAATCGAACATATT+TGG | - | chr3.3:71029020-71029039 | None:intergenic | 35.0% | |
| GCGAAAATCGAACATATTTG+GGG | - | chr3.3:71029018-71029037 | None:intergenic | 35.0% | |
| GTGCAATAGGCAAAATTTGT+TGG | + | chr3.3:71029092-71029111 | MS.gene75631:intron | 35.0% | |
| TAAGCCACACATGATAAAAG+GGG | - | chr3.3:71029400-71029419 | None:intergenic | 35.0% | |
| TCTTCATCAAATGGAACCTA+CGG | + | chr3.3:71029053-71029072 | MS.gene75631:intron | 35.0% | |
| ! | GTGGGTTATTGGATTGATTA+TGG | + | chr3.3:71028608-71028627 | MS.gene75631:CDS | 35.0% |
| ! | TATGTTCGATTTTCGCTGAT+TGG | + | chr3.3:71029022-71029041 | MS.gene75631:intron | 35.0% |
| ! | TTTCTTTTTCTGTGTTGGGT+TGG | + | chr3.3:71028921-71028940 | MS.gene75631:intron | 35.0% |
| !! | ATGTTCGATTTTCGCTGATT+GGG | + | chr3.3:71029023-71029042 | MS.gene75631:intron | 35.0% |
| !! | GGTCTTGTTTCTTCATCAAA+TGG | + | chr3.3:71029044-71029063 | MS.gene75631:intron | 35.0% |
| !!! | AGCTGCTTTGCTTTTTAAGA+AGG | - | chr3.3:71029299-71029318 | None:intergenic | 35.0% |
| AGGTGTATGTGAATTTCGTG+GGG | + | chr3.3:71029132-71029151 | MS.gene75631:intron | 40.0% | |
| CAAGGTGTATGTGAATTTCG+TGG | + | chr3.3:71029130-71029149 | MS.gene75631:intron | 40.0% | |
| CCTGGAATAGAATCTTTACG+CGG | - | chr3.3:71028720-71028739 | None:intergenic | 40.0% | |
| GTTACCACTCTCTCTGATAT+AGG | + | chr3.3:71028651-71028670 | MS.gene75631:CDS | 40.0% | |
| TGTTCTCTGTTATATCACCG+CGG | + | chr3.3:71028680-71028699 | MS.gene75631:CDS | 40.0% | |
| TTGGAGAAATTTAACGGCGT+TGG | - | chr3.3:71028447-71028466 | None:intergenic | 40.0% | |
| ! | AAATTTGTGTTGTTGCAGCG+TGG | + | chr3.3:71029426-71029445 | MS.gene75631:intron | 40.0% |
| ! | GCTTTTTGTTGCGTTGCATT+AGG | + | chr3.3:71029260-71029279 | MS.gene75631:intron | 40.0% |
| !!! | TGGATTTGTTTTGATGCCGT+GGG | + | chr3.3:71028590-71028609 | MS.gene75631:CDS | 40.0% |
| !!! | TTGGATTTGTTTTGATGCCG+TGG | + | chr3.3:71028589-71028608 | MS.gene75631:CDS | 40.0% |
| ACGACCTATATCAGAGAGAG+TGG | - | chr3.3:71028658-71028677 | None:intergenic | 45.0% | |
| CCACACATGATAAAAGGGGT+GGG | - | chr3.3:71029396-71029415 | None:intergenic | 45.0% | |
| CCGCGTAAAGATTCTATTCC+AGG | + | chr3.3:71028717-71028736 | MS.gene75631:CDS | 45.0% | |
| CTACGGTTTCGCTGAATTAG+AGG | + | chr3.3:71029070-71029089 | MS.gene75631:intron | 45.0% | |
| CTCTAATTCAGCGAAACCGT+AGG | - | chr3.3:71029072-71029091 | None:intergenic | 45.0% | |
| GGAATAGAATCTTTACGCGG+CGG | - | chr3.3:71028717-71028736 | None:intergenic | 45.0% | |
| GGATCGGTCACGTTCTTTAT+TGG | + | chr3.3:71028570-71028589 | MS.gene75631:CDS | 45.0% | |
| GGTTGAGAAGGATTGCAGAT+AGG | - | chr3.3:71028410-71028429 | None:intergenic | 45.0% | |
| TGGTGAGAGAACATAGGGAA+AGG | + | chr3.3:71028941-71028960 | MS.gene75631:intron | 45.0% | |
| ! | CGCTGAATTAGAGGTGCAAT+AGG | + | chr3.3:71029079-71029098 | MS.gene75631:intron | 45.0% |
| ! | GAGAAATTTAACGGCGTTGG+TGG | - | chr3.3:71028444-71028463 | None:intergenic | 45.0% |
| ! | TCTGCTTCTGGGAATTTCGA+TGG | + | chr3.3:71028533-71028552 | MS.gene75631:CDS | 45.0% |
| !! | GTTTTGATGCCGTGGGTTAT+TGG | + | chr3.3:71028597-71028616 | MS.gene75631:CDS | 45.0% |
| !! | TGGGTTGGTGAGAGAACATA+GGG | + | chr3.3:71028936-71028955 | MS.gene75631:intron | 45.0% |
| !! | TTGGGTTGGTGAGAGAACAT+AGG | + | chr3.3:71028935-71028954 | MS.gene75631:intron | 45.0% |
| AAACCCGCAGGTGAGATTTG+AGG | - | chr3.3:71028507-71028526 | None:intergenic | 50.0% | |
| ATAGAATCTTTACGCGGCGG+TGG | - | chr3.3:71028714-71028733 | None:intergenic | 50.0% | |
| CGAAATTCCCAGAAGCAGAG+AGG | - | chr3.3:71028532-71028551 | None:intergenic | 50.0% | |
| GATAAAAGGGGTGGGCAAGA+GGG | - | chr3.3:71029388-71029407 | None:intergenic | 50.0% | |
| GCCACACATGATAAAAGGGG+TGG | - | chr3.3:71029397-71029416 | None:intergenic | 50.0% | |
| TCCACCTCAAATCTCACCTG+CGG | + | chr3.3:71028500-71028519 | MS.gene75631:CDS | 50.0% | |
| TGATAAAAGGGGTGGGCAAG+AGG | - | chr3.3:71029389-71029408 | None:intergenic | 50.0% | |
| ! | CCCACCCCTTTTATCATGTG+TGG | + | chr3.3:71029393-71029412 | MS.gene75631:intron | 50.0% |
| ! | GGTTTTGCCTCTCTGCTTCT+GGG | + | chr3.3:71028522-71028541 | MS.gene75631:CDS | 50.0% |
| !! | TTAACGGCGTTGGTGGTGAA+CGG | - | chr3.3:71028437-71028456 | None:intergenic | 50.0% |
| AAACGCCGTAACGGTTGCTC+CGG | - | chr3.3:71028477-71028496 | None:intergenic | 55.0% | |
| ATCCACCGGAGCAACCGTTA+CGG | + | chr3.3:71028469-71028488 | MS.gene75631:CDS | 55.0% | |
| CCACCTCAAATCTCACCTGC+GGG | + | chr3.3:71028501-71028520 | MS.gene75631:CDS | 55.0% | |
| GGTGGAGGTAAACGCCGTAA+CGG | - | chr3.3:71028486-71028505 | None:intergenic | 55.0% | |
| GTGAACGGAGGAGGTTGAGA+AGG | - | chr3.3:71028422-71028441 | None:intergenic | 55.0% | |
| ! | GCAGGTGAGATTTGAGGTGG+AGG | - | chr3.3:71028501-71028520 | None:intergenic | 55.0% |
| ! | GGCTCTGATGCTCTGTGGAT+CGG | + | chr3.3:71028554-71028573 | MS.gene75631:CDS | 55.0% |
| ! | GGGTTTTGCCTCTCTGCTTC+TGG | + | chr3.3:71028521-71028540 | MS.gene75631:CDS | 55.0% |
| !! | TCGATGGCTCTGATGCTCTG+TGG | + | chr3.3:71028549-71028568 | MS.gene75631:CDS | 55.0% |
| AGCAGAGAGGCAAAACCCGC+AGG | - | chr3.3:71028519-71028538 | None:intergenic | 60.0% | |
| CCCGCAGGTGAGATTTGAGG+TGG | - | chr3.3:71028504-71028523 | None:intergenic | 60.0% | |
| CGGTTGCTCCGGTGGATGAT+TGG | - | chr3.3:71028466-71028485 | None:intergenic | 60.0% | |
| CTGTTATATCACCGCGGCGC+CGG | + | chr3.3:71028686-71028705 | MS.gene75631:CDS | 60.0% | |
| TCTTTACGCGGCGGTGGCTC+CGG | - | chr3.3:71028708-71028727 | None:intergenic | 65.0% | |
| !! | ACGGCGTTGGTGGTGAACGG+AGG | - | chr3.3:71028434-71028453 | None:intergenic | 65.0% |
| !! | GCGTTGGTGGTGAACGGAGG+AGG | - | chr3.3:71028431-71028450 | None:intergenic | 65.0% |
| CGCCGTAACGGTTGCTCCGG+TGG | - | chr3.3:71028474-71028493 | None:intergenic | 70.0% | |
| GGGCAAGAGGGACCGCAGCT+AGG | - | chr3.3:71029376-71029395 | None:intergenic | 70.0% | |
| ! | CGGCGGTGGCTCCGGCGCCG+CGG | - | chr3.3:71028700-71028719 | None:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 71028360 | 71029460 | 71028360 | ID=MS.gene75631 |
| chr3.3 | mRNA | 71028360 | 71029460 | 71028360 | ID=MS.gene75631.t1;Parent=MS.gene75631 |
| chr3.3 | exon | 71028360 | 71028738 | 71028360 | ID=MS.gene75631.t1.exon1;Parent=MS.gene75631.t1 |
| chr3.3 | CDS | 71028360 | 71028738 | 71028360 | ID=cds.MS.gene75631.t1;Parent=MS.gene75631.t1 |
| chr3.3 | exon | 71029444 | 71029460 | 71029444 | ID=MS.gene75631.t1.exon2;Parent=MS.gene75631.t1 |
| chr3.3 | CDS | 71029444 | 71029460 | 71029444 | ID=cds.MS.gene75631.t1;Parent=MS.gene75631.t1 |
| Gene Sequence |
| Protein sequence |
