Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75913.t1 | XP_003603592.1 | 93.5 | 170 | 5 | 1 | 1 | 164 | 1 | 170 | 2.20E-86 | 328.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75913.t1 | Q67XG0 | 69.6 | 171 | 39 | 2 | 1 | 158 | 1 | 171 | 2.3e-60 | 233.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75913.t1 | G7J2X4 | 93.5 | 170 | 5 | 1 | 1 | 164 | 1 | 170 | 1.6e-86 | 328.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene75913 | 0.805486 | 1.30E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene75913 | 0.818911 | 1.53E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene75913 | 0.805437 | 1.34E-49 | -1.69E-46 |
| MS.gene053900 | MS.gene75913 | 0.804149 | 2.48E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene75913 | 0.804532 | 2.06E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene75913 | 0.817612 | 3.01E-52 | -1.69E-46 |
| MS.gene060352 | MS.gene75913 | 0.814266 | 1.68E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75913.t1 | MTR_3g109420 | 93.529 | 170 | 5 | 1 | 1 | 164 | 1 | 170 | 5.72e-118 | 330 |
| MS.gene75913.t1 | MTR_1g008700 | 75.301 | 166 | 33 | 3 | 1 | 158 | 1 | 166 | 1.91e-85 | 248 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75913.t1 | AT4G32690 | 69.591 | 171 | 39 | 2 | 1 | 158 | 1 | 171 | 6.17e-81 | 237 |
Find 37 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAACCAGTACGAATTCT+TGG | 0.289012 | 3.3:-90035039 | MS.gene75913:CDS |
| ATAGAACCTGTTGTAGAAAT+TGG | 0.299176 | 3.3:+90035208 | None:intergenic |
| AAAGTTAATCAACATTATCT+TGG | 0.353946 | 3.3:+90034433 | None:intergenic |
| GCTTCATGTGTCACAGGAAA+TGG | 0.391868 | 3.3:+90034521 | None:intergenic |
| GAGGAGGACCTCCCATTCTC+TGG | 0.415467 | 3.3:+90035015 | None:intergenic |
| TGCTTTGTCTTTATCAGACT+TGG | 0.418611 | 3.3:+90035064 | None:intergenic |
| CGAATTCTTGGTCCAGAGAA+TGG | 0.418706 | 3.3:-90035027 | MS.gene75913:CDS |
| TTTCCACCAATTTCTACAAC+AGG | 0.420675 | 3.3:-90035214 | MS.gene75913:intron |
| CTTCCAGCTGCATCCTTGCA+AGG | 0.423236 | 3.3:+90034267 | None:intergenic |
| AAACAAGTTGGTGTCATCAA+TGG | 0.436122 | 3.3:+90035268 | None:intergenic |
| AAAGGTTAATGAAAGTTTGA+AGG | 0.487596 | 3.3:+90035234 | None:intergenic |
| ACTTCATATGCAACAAGCAT+TGG | 0.490000 | 3.3:-90034483 | MS.gene75913:CDS |
| TTCCAGCTGCATCCTTGCAA+GGG | 0.503260 | 3.3:+90034268 | None:intergenic |
| GAATTCTTGGTCCAGAGAAT+GGG | 0.505694 | 3.3:-90035026 | MS.gene75913:CDS |
| CACCCTTGCAAGGATGCAGC+TGG | 0.514180 | 3.3:-90034270 | MS.gene75913:CDS |
| TCTGGACCAAGAATTCGTAC+TGG | 0.524772 | 3.3:+90035033 | None:intergenic |
| TGTTGTAGAAATTGGTGGAA+AGG | 0.527682 | 3.3:+90035216 | None:intergenic |
| CTGCATCCTTGCAAGGGTGA+AGG | 0.528329 | 3.3:+90034274 | None:intergenic |
| TGCAAGAGAAGGCTTCTGAA+TGG | 0.530466 | 3.3:-90035319 | MS.gene75913:CDS |
| TCGGCAGCTTCATGTGTCAC+AGG | 0.536702 | 3.3:+90034515 | None:intergenic |
| GAACCTGTTGTAGAAATTGG+TGG | 0.539038 | 3.3:+90035211 | None:intergenic |
| GATGACGATGAGGAGTGGTT+TGG | 0.563976 | 3.3:-90035098 | MS.gene75913:CDS |
| CGATGTCTAGTAATTAGAGC+AGG | 0.568423 | 3.3:+90034545 | None:intergenic |
| CACATGAAGCTGCCGAGAGG+TGG | 0.582503 | 3.3:-90034508 | MS.gene75913:CDS |
| CCTCTGTATTCTCAGAGAAG+AGG | 0.592545 | 3.3:-90034996 | MS.gene75913:intron |
| TCAAAGCCTTCACCCTTGCA+AGG | 0.595706 | 3.3:-90034280 | MS.gene75913:CDS |
| ACCAACTTGTTTCAAAAGCT+AGG | 0.598000 | 3.3:-90035257 | MS.gene75913:CDS |
| CTTGCAAGGATGCAGCTGGA+AGG | 0.604226 | 3.3:-90034266 | MS.gene75913:CDS |
| GATGCAGAATCTGCAAGAGA+AGG | 0.605698 | 3.3:-90035330 | MS.gene75913:CDS |
| TTCTTGGTCCAGAGAATGGG+AGG | 0.632605 | 3.3:-90035023 | MS.gene75913:CDS |
| CATATGAAGTAACCACCTCT+CGG | 0.635321 | 3.3:+90034496 | None:intergenic |
| TAGAGTGTATGATGACGATG+AGG | 0.661998 | 3.3:-90035108 | MS.gene75913:CDS |
| GAGAAGGCTTCTGAATGGAG+TGG | 0.664521 | 3.3:-90035314 | MS.gene75913:CDS |
| TGTATGATGACGATGAGGAG+TGG | 0.684110 | 3.3:-90035103 | MS.gene75913:CDS |
| CCTCTTCTCTGAGAATACAG+AGG | 0.698670 | 3.3:+90034996 | None:intergenic |
| CTTCTCTGAGAATACAGAGG+AGG | 0.701310 | 3.3:+90034999 | None:intergenic |
| TGACACATGAAGCTGCCGAG+AGG | 0.786257 | 3.3:-90034511 | MS.gene75913:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTCAATAATATTCTTTATT+TGG | - | chr3.3:90034848-90034867 | MS.gene75913:intron | 15.0% |
| !! | TATATGACCTAAATATATAT+AGG | + | chr3.3:90034950-90034969 | None:intergenic | 15.0% |
| !!! | CTTTTAGATTTTTAACTTTT+CGG | - | chr3.3:90034645-90034664 | MS.gene75913:intron | 15.0% |
| !!! | GTCAATAATATTCTTTATTT+GGG | - | chr3.3:90034849-90034868 | MS.gene75913:intron | 15.0% |
| !!! | TCAATAATATTCTTTATTTG+GGG | - | chr3.3:90034850-90034869 | MS.gene75913:intron | 15.0% |
| !!! | TTTGAAACCTATATATATTT+AGG | - | chr3.3:90034940-90034959 | MS.gene75913:intron | 15.0% |
| !! | AAAGTTAATCAACATTATCT+TGG | + | chr3.3:90035144-90035163 | None:intergenic | 20.0% |
| !! | ATATATCTCTAATAAATGTG+TGG | - | chr3.3:90034967-90034986 | MS.gene75913:intron | 20.0% |
| ! | AAAGGTTAATGAAAGTTTGA+AGG | + | chr3.3:90034343-90034362 | None:intergenic | 25.0% |
| ! | ACTAAGTTCATGACAATTAA+TGG | - | chr3.3:90034432-90034451 | MS.gene75913:intron | 25.0% |
| ! | ATATATAGGTTTCAAAGCTT+AGG | + | chr3.3:90034936-90034955 | None:intergenic | 25.0% |
| !! | TAATATTCTTTATTTGGGGA+TGG | - | chr3.3:90034854-90034873 | MS.gene75913:intron | 25.0% |
| !!! | AACCTGTGTTTTTACTTTTT+AGG | - | chr3.3:90035230-90035249 | MS.gene75913:CDS | 25.0% |
| ATAGAACCTGTTGTAGAAAT+TGG | + | chr3.3:90034369-90034388 | None:intergenic | 30.0% | |
| GTCATGAACTTAGTAAAGAA+AGG | + | chr3.3:90034426-90034445 | None:intergenic | 30.0% | |
| TGCCTAAAAAGTAAAAACAC+AGG | + | chr3.3:90035235-90035254 | None:intergenic | 30.0% | |
| ! | TTGATTCTTCTTTTCAACTC+CGG | + | chr3.3:90035278-90035297 | None:intergenic | 30.0% |
| ACTTCATATGCAACAAGCAT+TGG | - | chr3.3:90035091-90035110 | MS.gene75913:CDS | 35.0% | |
| ATGTCCTGTAATGTGAAGTA+CGG | + | chr3.3:90035009-90035028 | None:intergenic | 35.0% | |
| TCAAAACCAGTACGAATTCT+TGG | - | chr3.3:90034535-90034554 | MS.gene75913:CDS | 35.0% | |
| TGCTTTGTCTTTATCAGACT+TGG | + | chr3.3:90034513-90034532 | None:intergenic | 35.0% | |
| TGTTGTAGAAATTGGTGGAA+AGG | + | chr3.3:90034361-90034380 | None:intergenic | 35.0% | |
| TTTCCACCAATTTCTACAAC+AGG | - | chr3.3:90034360-90034379 | MS.gene75913:intron | 35.0% | |
| ! | ACCAACTTGTTTCAAAAGCT+AGG | - | chr3.3:90034317-90034336 | MS.gene75913:CDS | 35.0% |
| ! | ACTGCATATTTTCTTGTTGC+CGG | - | chr3.3:90035256-90035275 | MS.gene75913:CDS | 35.0% |
| !! | AAACAAGTTGGTGTCATCAA+TGG | + | chr3.3:90034309-90034328 | None:intergenic | 35.0% |
| CATATGAAGTAACCACCTCT+CGG | + | chr3.3:90035081-90035100 | None:intergenic | 40.0% | |
| CGATGTCTAGTAATTAGAGC+AGG | + | chr3.3:90035032-90035051 | None:intergenic | 40.0% | |
| GAACCTGTTGTAGAAATTGG+TGG | + | chr3.3:90034366-90034385 | None:intergenic | 40.0% | |
| GAATTCTTGGTCCAGAGAAT+GGG | - | chr3.3:90034548-90034567 | MS.gene75913:CDS | 40.0% | |
| GGGATGGTTAGTAAAATGAC+AGG | - | chr3.3:90034870-90034889 | MS.gene75913:intron | 40.0% | |
| ! | TAGAGTGTATGATGACGATG+AGG | - | chr3.3:90034466-90034485 | MS.gene75913:CDS | 40.0% |
| !!! | GCCTAGCTTTTGAAACAAGT+TGG | + | chr3.3:90034321-90034340 | None:intergenic | 40.0% |
| CCTCTGTATTCTCAGAGAAG+AGG | - | chr3.3:90034578-90034597 | MS.gene75913:intron | 45.0% | |
| CGAATTCTTGGTCCAGAGAA+TGG | - | chr3.3:90034547-90034566 | MS.gene75913:CDS | 45.0% | |
| GCTACCGTACTTCACATTAC+AGG | - | chr3.3:90035002-90035021 | MS.gene75913:CDS | 45.0% | |
| GCTTCATGTGTCACAGGAAA+TGG | + | chr3.3:90035056-90035075 | None:intergenic | 45.0% | |
| TCTGGACCAAGAATTCGTAC+TGG | + | chr3.3:90034544-90034563 | None:intergenic | 45.0% | |
| TGCAAGAGAAGGCTTCTGAA+TGG | - | chr3.3:90034255-90034274 | MS.gene75913:CDS | 45.0% | |
| ! | CCTCTTCTCTGAGAATACAG+AGG | + | chr3.3:90034581-90034600 | None:intergenic | 45.0% |
| ! | CTTCTCTGAGAATACAGAGG+AGG | + | chr3.3:90034578-90034597 | None:intergenic | 45.0% |
| ! | TGTATGATGACGATGAGGAG+TGG | - | chr3.3:90034471-90034490 | MS.gene75913:CDS | 45.0% |
| GAGAAGGCTTCTGAATGGAG+TGG | - | chr3.3:90034260-90034279 | MS.gene75913:CDS | 50.0% | |
| GATGACGATGAGGAGTGGTT+TGG | - | chr3.3:90034476-90034495 | MS.gene75913:CDS | 50.0% | |
| TCAAAGCCTTCACCCTTGCA+AGG | - | chr3.3:90035294-90035313 | MS.gene75913:CDS | 50.0% | |
| TTCCAGCTGCATCCTTGCAA+GGG | + | chr3.3:90035309-90035328 | None:intergenic | 50.0% | |
| TTCTTGGTCCAGAGAATGGG+AGG | - | chr3.3:90034551-90034570 | MS.gene75913:CDS | 50.0% | |
| CTGCATCCTTGCAAGGGTGA+AGG | + | chr3.3:90035303-90035322 | None:intergenic | 55.0% | |
| CTTCCAGCTGCATCCTTGCA+AGG | + | chr3.3:90035310-90035329 | None:intergenic | 55.0% | |
| CTTGCAAGGATGCAGCTGGA+AGG | - | chr3.3:90035308-90035327 | MS.gene75913:CDS | 55.0% | |
| TCGGCAGCTTCATGTGTCAC+AGG | + | chr3.3:90035062-90035081 | None:intergenic | 55.0% | |
| TGACACATGAAGCTGCCGAG+AGG | - | chr3.3:90035063-90035082 | MS.gene75913:CDS | 55.0% | |
| CACATGAAGCTGCCGAGAGG+TGG | - | chr3.3:90035066-90035085 | MS.gene75913:CDS | 60.0% | |
| CACCCTTGCAAGGATGCAGC+TGG | - | chr3.3:90035304-90035323 | MS.gene75913:CDS | 60.0% | |
| GAGGAGGACCTCCCATTCTC+TGG | + | chr3.3:90034562-90034581 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90034245 | 90035351 | 90034245 | ID=MS.gene75913 |
| chr3.3 | mRNA | 90034245 | 90035351 | 90034245 | ID=MS.gene75913.t1;Parent=MS.gene75913 |
| chr3.3 | exon | 90035215 | 90035351 | 90035215 | ID=MS.gene75913.t1.exon1;Parent=MS.gene75913.t1 |
| chr3.3 | CDS | 90035215 | 90035351 | 90035215 | ID=cds.MS.gene75913.t1;Parent=MS.gene75913.t1 |
| chr3.3 | exon | 90034997 | 90035127 | 90034997 | ID=MS.gene75913.t1.exon2;Parent=MS.gene75913.t1 |
| chr3.3 | CDS | 90034997 | 90035127 | 90034997 | ID=cds.MS.gene75913.t1;Parent=MS.gene75913.t1 |
| chr3.3 | exon | 90034446 | 90034572 | 90034446 | ID=MS.gene75913.t1.exon3;Parent=MS.gene75913.t1 |
| chr3.3 | CDS | 90034446 | 90034572 | 90034446 | ID=cds.MS.gene75913.t1;Parent=MS.gene75913.t1 |
| chr3.3 | exon | 90034245 | 90034344 | 90034245 | ID=MS.gene75913.t1.exon4;Parent=MS.gene75913.t1 |
| chr3.3 | CDS | 90034245 | 90034344 | 90034245 | ID=cds.MS.gene75913.t1;Parent=MS.gene75913.t1 |
| Gene Sequence |
| Protein sequence |
