AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060352


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060352.t1 MTR_4g118720 95.593 295 13 0 2 296 2988 3282 6.49e-175 536
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACCGATGCTGAGGAATT+AGG 0.208543 8.4:-12812407 None:intergenic
AGAAGTTAATTCCTTGCTTT+TGG 0.214754 8.4:-12812073 None:intergenic
GTTGCAGAGGACATCCCTTT+TGG 0.274645 8.4:-12812133 None:intergenic
CGCTCTGCCTTCTTGCTCGC+TGG 0.348500 8.4:+12811781 MS.gene060352:CDS
AGATAATGATGTGTTAATTC+AGG 0.379885 8.4:+12811811 MS.gene060352:CDS
TGAGATGGTTCATCAAATAA+AGG 0.400520 8.4:+12812014 MS.gene060352:CDS
TCTGATTTCTCCTGTGGTAA+AGG 0.430463 8.4:-12811979 None:intergenic
ACAATTTCTCTGATCTGCAT+TGG 0.432368 8.4:-12811591 None:intergenic
ATCAGAGAAATTGTTCTGAT+AGG 0.442703 8.4:+12811600 MS.gene060352:CDS
TTGAGATACATTTGCAATCT+CGG 0.447612 8.4:-12812381 None:intergenic
CTAGAGGGAAGTTGTATAGA+TGG 0.450093 8.4:+12811857 MS.gene060352:CDS
TGACCAGTCTCGACTTCTAA+AGG 0.453149 8.4:-12812317 None:intergenic
TCACCCTCAGGTTCAGAAAC+GGG 0.458247 8.4:-12811728 None:intergenic
GCCTTCTTGCTCGCTGGTGA+AGG 0.462218 8.4:+12811787 MS.gene060352:CDS
AGAAAATGAAGAGAATCAAA+TGG 0.463269 8.4:+12812170 MS.gene060352:CDS
TTGTCTGAGAAGGATTCAAT+TGG 0.469260 8.4:+12812554 MS.gene060352:CDS
AGCCTTTCTGGATCACCCTC+AGG 0.472209 8.4:-12811740 None:intergenic
GCTAAAATGCAGGCTGATGT+AGG 0.478865 8.4:+12812473 MS.gene060352:CDS
TTCCTCAGCATCGGTCGAAA+AGG 0.486549 8.4:+12812412 MS.gene060352:CDS
AAGTTGATGCATCACCCAAA+AGG 0.488992 8.4:+12812118 MS.gene060352:CDS
GTTAATTCAGGACGAACAAA+AGG 0.496497 8.4:+12811823 MS.gene060352:CDS
ATTAACTTCTTCGTCAGTGA+TGG 0.504966 8.4:+12812086 MS.gene060352:CDS
CATTGCCAGTCCTTTACCAC+AGG 0.507595 8.4:+12811969 MS.gene060352:CDS
ATCACCCTCAGGTTCAGAAA+CGG 0.512036 8.4:-12811729 None:intergenic
AGTTGATGCATCACCCAAAA+GGG 0.515241 8.4:+12812119 MS.gene060352:CDS
GACTGTGACATGGTTGATCC+AGG 0.516379 8.4:+12812042 MS.gene060352:CDS
CAACTTCCCTCTAGAATCAA+AGG 0.523856 8.4:-12811848 None:intergenic
TGTGGCCGACAGTCACTAGA+TGG 0.525915 8.4:-12811634 None:intergenic
AAAGGATCCTTTGATTCTAG+AGG 0.526482 8.4:+12811841 MS.gene060352:CDS
AGAATGCTCTGAAGCTGAGA+TGG 0.526821 8.4:+12811999 MS.gene060352:CDS
GAGCATTCTGATTTCTCCTG+TGG 0.536023 8.4:-12811985 None:intergenic
CTGTGGTAAAGGACTGGCAA+TGG 0.548693 8.4:-12811968 None:intergenic
AACCTGAGGGTGATCCAGAA+AGG 0.553280 8.4:+12811738 MS.gene060352:CDS
AGCTCCTAATTCCTCAGCAT+CGG 0.556937 8.4:+12812403 MS.gene060352:CDS
GAATCAAATGGATGATAATG+AGG 0.561262 8.4:+12812182 MS.gene060352:CDS
ATGCAGGCTGATGTAGGAGA+TGG 0.563305 8.4:+12812479 MS.gene060352:CDS
TACTCCCGTTTCTGAACCTG+AGG 0.565621 8.4:+12811724 MS.gene060352:CDS
GAGGAAGGCTTATCTGAGAA+AGG 0.566536 8.4:+12812434 MS.gene060352:CDS
TGAAAGCACAGCTAAAATGC+AGG 0.568502 8.4:+12812463 MS.gene060352:CDS
AAGGATCCTTTGATTCTAGA+GGG 0.568780 8.4:+12811842 MS.gene060352:CDS
CTCAGCATCGGTCGAAAAGG+AGG 0.569242 8.4:+12812415 MS.gene060352:CDS
AACTTCTTCGTCAGTGATGG+AGG 0.569897 8.4:+12812089 MS.gene060352:CDS
GGCTGATGTAGGAGATGGAA+TGG 0.571153 8.4:+12812484 MS.gene060352:CDS
GCTTGTTGAGTTGTCTGAGA+AGG 0.575729 8.4:+12812544 MS.gene060352:CDS
AAGCCTTTAGAAGTCGAGAC+TGG 0.586788 8.4:+12812314 MS.gene060352:CDS
GAGGATGCATTGTCGATGAA+AGG 0.587975 8.4:+12811662 MS.gene060352:CDS
TAGCAGCAAGTGGGTTGCAG+AGG 0.593160 8.4:-12812146 None:intergenic
TTTCTCCTGTGGTAAAGGAC+TGG 0.595886 8.4:-12811974 None:intergenic
GCATCGGTCGAAAAGGAGGA+AGG 0.611644 8.4:+12812419 MS.gene060352:CDS
GCCGACAGTCACTAGATGGT+CGG 0.612454 8.4:-12811630 None:intergenic
AGGATTGACATCCAAAAGCA+AGG 0.614984 8.4:+12812062 MS.gene060352:CDS
TCCTTCACCAGCGAGCAAGA+AGG 0.623740 8.4:-12811788 None:intergenic
GACTGTCGGCCACACTAGTG+AGG 0.645688 8.4:+12811643 MS.gene060352:CDS
TCCGACCATCTAGTGACTGT+CGG 0.650244 8.4:+12811629 MS.gene060352:CDS
ACAATGCATCCTCACTAGTG+TGG 0.657647 8.4:-12811652 None:intergenic
CGAGACTGGTCATCAAATCG+AGG 0.673178 8.4:+12812328 MS.gene060352:CDS
ACTCCCGTTTCTGAACCTGA+GGG 0.686794 8.4:+12811725 MS.gene060352:CDS
AAAGGTATCTGACTGTGACA+TGG 0.689682 8.4:+12812032 MS.gene060352:CDS
AAGTTGTATAGATGGCACCA+AGG 0.735776 8.4:+12811865 MS.gene060352:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAATAGATTTTATTTAAT+TGG - chr8.4:12812281-12812300 None:intergenic 10.0%
!! ATAAAATCTATTAACATTAC+AGG + chr8.4:12812286-12812305 MS.gene060352:intron 15.0%
!! AATGAATTTGTGAAATTGAA+TGG - chr8.4:12812248-12812267 None:intergenic 20.0%
!!! TTCACAAATTCATTTCTTTT+GGG + chr8.4:12812254-12812273 MS.gene060352:intron 20.0%
!!! TTTCACAAATTCATTTCTTT+TGG + chr8.4:12812253-12812272 MS.gene060352:intron 20.0%
! AGAAAATGAAGAGAATCAAA+TGG + chr8.4:12812170-12812189 MS.gene060352:CDS 25.0%
! AGATAATGATGTGTTAATTC+AGG + chr8.4:12811811-12811830 MS.gene060352:CDS 25.0%
! GAAATTGAATGGAAAATACA+AGG - chr8.4:12812237-12812256 None:intergenic 25.0%
ATCAGAGAAATTGTTCTGAT+AGG + chr8.4:12811600-12811619 MS.gene060352:CDS 30.0%
TGAGATGGTTCATCAAATAA+AGG + chr8.4:12812014-12812033 MS.gene060352:CDS 30.0%
TTGAGATACATTTGCAATCT+CGG - chr8.4:12812384-12812403 None:intergenic 30.0%
! AGAAGTTAATTCCTTGCTTT+TGG - chr8.4:12812076-12812095 None:intergenic 30.0%
! GAATCAAATGGATGATAATG+AGG + chr8.4:12812182-12812201 MS.gene060352:CDS 30.0%
! GATGATAATGAGGTATTTCA+TGG + chr8.4:12812192-12812211 MS.gene060352:intron 30.0%
! TTTTTAGATGTAAGCCTTTC+TGG - chr8.4:12811755-12811774 None:intergenic 30.0%
AAAGGATCCTTTGATTCTAG+AGG + chr8.4:12811841-12811860 MS.gene060352:CDS 35.0%
AAGGATCCTTTGATTCTAGA+GGG + chr8.4:12811842-12811861 MS.gene060352:CDS 35.0%
ACAATTTCTCTGATCTGCAT+TGG - chr8.4:12811594-12811613 None:intergenic 35.0%
ATTAACTTCTTCGTCAGTGA+TGG + chr8.4:12812086-12812105 MS.gene060352:CDS 35.0%
GTTAATTCAGGACGAACAAA+AGG + chr8.4:12811823-12811842 MS.gene060352:CDS 35.0%
TTGTCTGAGAAGGATTCAAT+TGG + chr8.4:12812554-12812573 MS.gene060352:CDS 35.0%
AAGTTGTATAGATGGCACCA+AGG + chr8.4:12811865-12811884 MS.gene060352:CDS 40.0%
AGGATTGACATCCAAAAGCA+AGG + chr8.4:12812062-12812081 MS.gene060352:CDS 40.0%
CTAGAGGGAAGTTGTATAGA+TGG + chr8.4:12811857-12811876 MS.gene060352:CDS 40.0%
TCTGATTTCTCCTGTGGTAA+AGG - chr8.4:12811982-12812001 None:intergenic 40.0%
TGACTTTATCCCGACTTCAT+AGG + chr8.4:12811936-12811955 MS.gene060352:intron 40.0%
! AAGGTTCTAACATGTGCCAA+TGG - chr8.4:12812218-12812237 None:intergenic 40.0%
! AGTTGATGCATCACCCAAAA+GGG + chr8.4:12812119-12812138 MS.gene060352:CDS 40.0%
! ATGAGGTATTTCATGGCCAT+TGG + chr8.4:12812199-12812218 MS.gene060352:intron 40.0%
! CATTTTCTGTAGCAGCAAGT+GGG - chr8.4:12812158-12812177 None:intergenic 40.0%
! TCATTTTCTGTAGCAGCAAG+TGG - chr8.4:12812159-12812178 None:intergenic 40.0%
! TGAAAGCACAGCTAAAATGC+AGG + chr8.4:12812463-12812482 MS.gene060352:CDS 40.0%
!! AAAGGTATCTGACTGTGACA+TGG + chr8.4:12812032-12812051 MS.gene060352:CDS 40.0%
!! AAGTTGATGCATCACCCAAA+AGG + chr8.4:12812118-12812137 MS.gene060352:CDS 40.0%
!! CAACTTCCCTCTAGAATCAA+AGG - chr8.4:12811851-12811870 None:intergenic 40.0%
!! TTGCTTTTGGATGTCAATCC+TGG - chr8.4:12812063-12812082 None:intergenic 40.0%
AACTTCTTCGTCAGTGATGG+AGG + chr8.4:12812089-12812108 MS.gene060352:CDS 45.0%
AAGTCGGGATAAAGTCAGAG+AGG - chr8.4:12811933-12811952 None:intergenic 45.0%
AAGTGCAACGTGACATACCT+TGG - chr8.4:12811885-12811904 None:intergenic 45.0%
AATCCTGCACCTATGAAGTC+GGG - chr8.4:12811948-12811967 None:intergenic 45.0%
ACAATGCATCCTCACTAGTG+TGG - chr8.4:12811655-12811674 None:intergenic 45.0%
AGCTCCTAATTCCTCAGCAT+CGG + chr8.4:12812403-12812422 MS.gene060352:CDS 45.0%
AGTCGGGATAAAGTCAGAGA+GGG - chr8.4:12811932-12811951 None:intergenic 45.0%
ATCACCCTCAGGTTCAGAAA+CGG - chr8.4:12811732-12811751 None:intergenic 45.0%
GAATCCTGCACCTATGAAGT+CGG - chr8.4:12811949-12811968 None:intergenic 45.0%
GAGCATTCTGATTTCTCCTG+TGG - chr8.4:12811988-12812007 None:intergenic 45.0%
GAGGAAGGCTTATCTGAGAA+AGG + chr8.4:12812434-12812453 MS.gene060352:CDS 45.0%
GAGGATGCATTGTCGATGAA+AGG + chr8.4:12811662-12811681 MS.gene060352:CDS 45.0%
GCTAAAATGCAGGCTGATGT+AGG + chr8.4:12812473-12812492 MS.gene060352:CDS 45.0%
TGACCAGTCTCGACTTCTAA+AGG - chr8.4:12812320-12812339 None:intergenic 45.0%
TTTCTCCTGTGGTAAAGGAC+TGG - chr8.4:12811977-12811996 None:intergenic 45.0%
! AAGCCTTTAGAAGTCGAGAC+TGG + chr8.4:12812314-12812333 MS.gene060352:CDS 45.0%
! GCTTGTTGAGTTGTCTGAGA+AGG + chr8.4:12812544-12812563 MS.gene060352:CDS 45.0%
! TTGCAGAGGACATCCCTTTT+GGG - chr8.4:12812135-12812154 None:intergenic 45.0%
!! AGAATGCTCTGAAGCTGAGA+TGG + chr8.4:12811999-12812018 MS.gene060352:CDS 45.0%
AACCTGAGGGTGATCCAGAA+AGG + chr8.4:12811738-12811757 MS.gene060352:CDS 50.0%
ACTCCCGTTTCTGAACCTGA+GGG + chr8.4:12811725-12811744 MS.gene060352:CDS 50.0%
ATGCAGGCTGATGTAGGAGA+TGG + chr8.4:12812479-12812498 MS.gene060352:CDS 50.0%
CATTGCCAGTCCTTTACCAC+AGG + chr8.4:12811969-12811988 MS.gene060352:CDS 50.0%
GACTGTGACATGGTTGATCC+AGG + chr8.4:12812042-12812061 MS.gene060352:CDS 50.0%
GGCTGATGTAGGAGATGGAA+TGG + chr8.4:12812484-12812503 MS.gene060352:CDS 50.0%
TACTCCCGTTTCTGAACCTG+AGG + chr8.4:12811724-12811743 MS.gene060352:CDS 50.0%
TATCCCGACTTCATAGGTGC+AGG + chr8.4:12811942-12811961 MS.gene060352:intron 50.0%
TCACCCTCAGGTTCAGAAAC+GGG - chr8.4:12811731-12811750 None:intergenic 50.0%
TCCGACCATCTAGTGACTGT+CGG + chr8.4:12811629-12811648 MS.gene060352:CDS 50.0%
TCGACCGATGCTGAGGAATT+AGG - chr8.4:12812410-12812429 None:intergenic 50.0%
! CGAGACTGGTCATCAAATCG+AGG + chr8.4:12812328-12812347 MS.gene060352:CDS 50.0%
! CTGTGGTAAAGGACTGGCAA+TGG - chr8.4:12811971-12811990 None:intergenic 50.0%
! GTTGCAGAGGACATCCCTTT+TGG - chr8.4:12812136-12812155 None:intergenic 50.0%
!! TTCCTCAGCATCGGTCGAAA+AGG + chr8.4:12812412-12812431 MS.gene060352:CDS 50.0%
AGCCTTTCTGGATCACCCTC+AGG - chr8.4:12811743-12811762 None:intergenic 55.0%
GCCGACAGTCACTAGATGGT+CGG - chr8.4:12811633-12811652 None:intergenic 55.0%
TCCTTCACCAGCGAGCAAGA+AGG - chr8.4:12811791-12811810 None:intergenic 55.0%
TGTGGCCGACAGTCACTAGA+TGG - chr8.4:12811637-12811656 None:intergenic 55.0%
! CTCCTTTTCGACCGATGCTG+AGG - chr8.4:12812417-12812436 None:intergenic 55.0%
! GCATCGGTCGAAAAGGAGGA+AGG + chr8.4:12812419-12812438 MS.gene060352:CDS 55.0%
!! CTCAGCATCGGTCGAAAAGG+AGG + chr8.4:12812415-12812434 MS.gene060352:CDS 55.0%
!! TAGCAGCAAGTGGGTTGCAG+AGG - chr8.4:12812149-12812168 None:intergenic 55.0%
GACTGTCGGCCACACTAGTG+AGG + chr8.4:12811643-12811662 MS.gene060352:CDS 60.0%
GCCTTCTTGCTCGCTGGTGA+AGG + chr8.4:12811787-12811806 MS.gene060352:CDS 60.0%
CGCTCTGCCTTCTTGCTCGC+TGG + chr8.4:12811781-12811800 MS.gene060352:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 12811581 12812646 12811581 ID=MS.gene060352
chr8.4 mRNA 12811581 12812646 12811581 ID=MS.gene060352.t1;Parent=MS.gene060352
chr8.4 exon 12811581 12811886 12811581 ID=MS.gene060352.t1.exon1;Parent=MS.gene060352.t1
chr8.4 CDS 12811581 12811886 12811581 ID=cds.MS.gene060352.t1;Parent=MS.gene060352.t1
chr8.4 exon 12811958 12812203 12811958 ID=MS.gene060352.t1.exon2;Parent=MS.gene060352.t1
chr8.4 CDS 12811958 12812203 12811958 ID=cds.MS.gene060352.t1;Parent=MS.gene060352.t1
chr8.4 exon 12812308 12812646 12812308 ID=MS.gene060352.t1.exon3;Parent=MS.gene060352.t1
chr8.4 CDS 12812308 12812646 12812308 ID=cds.MS.gene060352.t1;Parent=MS.gene060352.t1
Gene Sequence

>MS.gene060352

ATGGAGGAATCCAATGCAGATCAGAGAAATTGTTCTGATAGGTTGCAATCCGACCATCTAGTGACTGTCGGCCACACTAGTGAGGATGCATTGTCGATGAAAGGCACAAAATTAGAAGTTGAAATTAGCGATAAAATAAATGCTACTCCCGTTTCTGAACCTGAGGGTGATCCAGAAAGGCTTACATCTAAAAACATTGACGCTCTGCCTTCTTGCTCGCTGGTGAAGGAAGATAATGATGTGTTAATTCAGGACGAACAAAAGGATCCTTTGATTCTAGAGGGAAGTTGTATAGATGGCACCAAGGTATGTCACGTTGCACTTTTTAGCACTTCTGACTCTGTAATTCCCTCTCTGACTTTATCCCGACTTCATAGGTGCAGGATTCCATTGCCAGTCCTTTACCACAGGAGAAATCAGAATGCTCTGAAGCTGAGATGGTTCATCAAATAAAGGTATCTGACTGTGACATGGTTGATCCAGGATTGACATCCAAAAGCAAGGAATTAACTTCTTCGTCAGTGATGGAGGAAGATAAAGTTGATGCATCACCCAAAAGGGATGTCCTCTGCAACCCACTTGCTGCTACAGAAAATGAAGAGAATCAAATGGATGATAATGAGGTATTTCATGGCCATTGGCACATGTTAGAACCTTGTATTTTCCATTCAATTTCACAAATTCATTTCTTTTGGGACCAATTAAATAAAATCTATTAACATTACAGGAATCAAAGCCTTTAGAAGTCGAGACTGGTCATCAAATCGAGGCATCTACAGATATTTCTGAAAGCAGTGCTGCCGAGATTGCAAATGTATCTCAAGCTCCTAATTCCTCAGCATCGGTCGAAAAGGAGGAAGGCTTATCTGAGAAAGGTGTAGATGAAAGCACAGCTAAAATGCAGGCTGATGTAGGAGATGGAATGGATATTTCATCTGTATGCTCTTCAGCAGCAGTGAGTGAGCTTGTTGAGTTGTCTGAGAAGGATTCAATTGGAAACTCTGAAACTGATGTAACTAAAGAGAAAGATGACGTCGCACAAGAAGAAAATGCATCAAAAGACTGA

Protein sequence

>MS.gene060352.t1

MEESNADQRNCSDRLQSDHLVTVGHTSEDALSMKGTKLEVEISDKINATPVSEPEGDPERLTSKNIDALPSCSLVKEDNDVLIQDEQKDPLILEGSCIDGTKVQDSIASPLPQEKSECSEAEMVHQIKVSDCDMVDPGLTSKSKELTSSSVMEEDKVDASPKRDVLCNPLAATENEENQMDDNEESKPLEVETGHQIEASTDISESSAAEIANVSQAPNSSASVEKEEGLSEKGVDESTAKMQADVGDGMDISSVCSSAAVSELVELSEKDSIGNSETDVTKEKDDVAQEENASKD