AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76516

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76516.t1 MTR_2g101520 98.480 329 5 0 1 329 1 329 0.0 671
MS.gene76516.t1 MTR_6g488100 47.399 346 128 11 1 329 14 322 7.03e-88 266
MS.gene76516.t1 MTR_6g488100 47.009 351 127 12 1 329 14 327 1.46e-86 263
MS.gene76516.t1 MTR_8g103065 47.578 351 132 12 1 329 15 335 1.09e-76 238
MS.gene76516.t1 MTR_6g488100 43.902 287 110 10 1 270 14 266 2.77e-59 192
MS.gene76516.t1 MTR_6g488100 44.061 261 97 9 1 244 14 242 1.38e-52 174
MS.gene76516.t1 MTR_8g103065 43.728 279 105 12 1 257 15 263 4.16e-43 150
MS.gene76516.t1 MTR_2g081820 47.368 152 49 3 175 326 312 432 3.21e-34 130
MS.gene76516.t1 MTR_2g081820 47.368 152 49 3 175 326 313 433 3.25e-34 130
MS.gene76516.t1 MTR_2g011070 45.625 160 55 3 167 326 283 410 5.37e-32 124
MS.gene76516.t1 MTR_1g107185 45.695 151 60 3 175 324 138 267 7.05e-32 120
MS.gene76516.t1 MTR_8g466800 46.452 155 52 3 175 329 320 443 4.15e-31 122
MS.gene76516.t1 MTR_3g112170 45.390 141 47 4 188 326 339 451 1.00e-29 118
MS.gene76516.t1 MTR_4g087300 44.444 135 35 2 189 323 33 127 1.21e-26 102
MS.gene76516.t1 MTR_4g087300 44.444 135 35 2 189 323 56 150 1.39e-26 103
MS.gene76516.t1 MTR_1g094085 42.208 154 55 4 175 325 405 527 5.75e-26 108
MS.gene76516.t1 MTR_4g087300 44.444 135 33 3 189 323 56 148 1.16e-24 98.2
MS.gene76516.t1 MTR_8g466800 64.286 70 25 0 175 244 320 389 6.37e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76516.t1 AT4G29100 41.895 401 152 15 1 329 15 406 5.59e-70 223
MS.gene76516.t1 AT2G20100 59.322 177 58 4 163 329 189 361 6.54e-60 196
MS.gene76516.t1 AT2G20100 47.085 223 58 5 163 329 189 407 8.78e-51 174
MS.gene76516.t1 AT4G29100 38.462 338 131 13 1 270 15 343 1.15e-42 151
MS.gene76516.t1 AT2G20100 64.035 114 31 3 163 270 189 298 2.10e-36 134
MS.gene76516.t1 AT2G20100 64.035 114 31 3 163 270 189 298 2.10e-36 134
MS.gene76516.t1 AT1G27660 51.773 141 36 4 173 311 316 426 1.18e-33 129
MS.gene76516.t1 AT3G19500 48.000 150 45 3 175 324 140 256 1.01e-32 122
MS.gene76516.t1 AT3G20640 43.871 155 55 3 175 329 269 391 9.52e-31 120
MS.gene76516.t1 AT3G20640 43.871 155 55 3 175 329 331 453 2.38e-30 120
MS.gene76516.t1 AT1G61660 44.079 152 56 2 175 326 263 385 4.93e-28 112
MS.gene76516.t1 AT1G61660 44.079 152 56 2 175 326 266 388 5.03e-28 112
MS.gene76516.t1 AT1G61660 44.079 152 56 2 175 326 266 388 5.03e-28 112
MS.gene76516.t1 AT2G31730 40.441 136 41 1 189 324 39 134 2.97e-26 102
MS.gene76516.t1 AT2G31730 40.441 136 41 1 189 324 39 134 2.97e-26 102
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 29 124 2.93e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT1G05710 39.706 136 42 1 189 324 37 132 3.50e-25 99.4
MS.gene76516.t1 AT4G05170 44.030 134 51 3 175 306 41 152 6.73e-25 99.4
MS.gene76516.t1 AT1G31050 41.358 162 64 5 166 325 300 432 2.09e-24 103
MS.gene76516.t1 AT1G31050 41.358 162 64 5 166 325 296 428 2.14e-24 103
MS.gene76516.t1 AT1G31050 41.358 162 64 5 166 325 296 428 2.55e-24 102
MS.gene76516.t1 AT4G05170 44.030 134 51 3 175 306 134 245 2.97e-24 100
MS.gene76516.t1 AT4G05170 44.030 134 51 3 175 306 159 270 3.61e-24 100
MS.gene76516.t1 AT4G05170 44.030 134 51 3 175 306 177 288 3.92e-24 100
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 29 146 3.55e-23 94.4
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 29 146 3.55e-23 94.4
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 37 154 5.02e-23 94.4
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 37 154 5.02e-23 94.4
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 37 154 5.02e-23 94.4
MS.gene76516.t1 AT1G05710 42.647 136 60 4 189 324 37 154 5.02e-23 94.4
MS.gene76516.t1 AT4G21340 47.445 137 44 5 175 309 176 286 9.35e-23 96.7
MS.gene76516.t1 AT4G05170 64.789 71 25 0 175 245 159 229 9.80e-22 92.4
MS.gene76516.t1 AT3G19500 65.152 66 23 0 175 240 140 205 7.47e-21 89.7
MS.gene76516.t1 AT1G61660 64.789 71 25 0 175 245 266 336 1.40e-20 91.3
MS.gene76516.t1 AT1G61660 64.789 71 25 0 175 245 266 336 1.40e-20 91.3
MS.gene76516.t1 AT1G49830 40.945 127 41 3 199 325 134 226 3.96e-20 88.6
MS.gene76516.t1 AT1G49830 40.945 127 41 3 199 325 133 225 4.01e-20 88.2
MS.gene76516.t1 AT3G20640 61.972 71 26 1 175 245 331 400 5.51e-20 90.5
MS.gene76516.t1 AT4G05170 70.690 58 17 0 175 232 159 216 1.65e-19 85.9
MS.gene76516.t1 AT4G05170 70.690 58 17 0 175 232 159 216 1.65e-19 85.9
MS.gene76516.t1 AT3G20640 63.793 58 20 1 175 232 331 387 2.70e-15 76.6
MS.gene76516.t1 AT1G61660 34.641 153 69 3 174 326 245 366 6.94e-14 72.0
MS.gene76516.t1 AT1G31050 52.857 70 32 1 166 234 296 365 2.06e-13 70.9
MS.gene76516.t1 AT1G31050 52.857 70 32 1 166 234 300 369 2.28e-13 70.5

Find 82 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCAAGAGTTCCATTTA+TGG 0.161942 2.3:-2579942 MS.gene76516:CDS
TGCTTTATTATATCCATAAA+TGG 0.195130 2.3:+2579929 None:intergenic
CAACATGTTGGAAGTGAAAA+TGG 0.269929 2.3:-2577556 MS.gene76516:CDS
GTGAAAATGGAGCTGATTAC+TGG 0.289834 2.3:-2577543 MS.gene76516:CDS
TGTGAGTAATTAACCTTTAG+AGG 0.294265 2.3:+2579453 None:intergenic
CTTAAGTAGTGATAATATAT+TGG 0.313482 2.3:-2579771 MS.gene76516:CDS
CATACCTGACCGGGGTCTTC+AGG 0.315826 2.3:+2578394 None:intergenic
AAGAATACCTGGTTTGGAAT+TGG 0.331532 2.3:+2578049 None:intergenic
CACCAGCTAGTTTCTCCATT+TGG 0.331535 2.3:-2579135 MS.gene76516:CDS
ACAGAATGCAGAAGGTAATA+AGG 0.336565 2.3:-2577629 MS.gene76516:intron
GATTACTGGGCACCAGCATT+TGG 0.361562 2.3:-2577529 MS.gene76516:CDS
AAAGAAAAGAATACCTGGTT+TGG 0.370691 2.3:+2578043 None:intergenic
TGGTTCATCTTGGTCACATA+TGG 0.375811 2.3:-2579828 MS.gene76516:CDS
GGATGCATGCTCCACCAGTT+AGG 0.382855 2.3:+2580437 None:intergenic
AGCTAGTGATATGAGAGCTT+GGG 0.388897 2.3:+2579792 None:intergenic
TGATGGCTGGGAACCCTAAC+TGG 0.390562 2.3:-2580451 MS.gene76516:CDS
GGCACTCAGCACCCCTTACT+TGG 0.395598 2.3:-2578648 MS.gene76516:CDS
CTGGATCACTTGGTTCATCT+TGG 0.397211 2.3:-2579838 MS.gene76516:CDS
GATGCTGCAGTGTCCAAGTA+AGG 0.413970 2.3:+2578635 None:intergenic
TGAGAGCTTGGGGAAGAAAC+TGG 0.431844 2.3:+2579803 None:intergenic
TAGAAGCCATTGGATACATC+AGG 0.435008 2.3:-2578863 MS.gene76516:CDS
TTTACCTCAGATATTTGATC+TGG 0.452488 2.3:+2579707 None:intergenic
GCAAAGAACTTGAAGAGTAG+AGG 0.454514 2.3:-2577607 MS.gene76516:CDS
CGTGCTAACTCTGGATCACT+TGG 0.461704 2.3:-2579848 MS.gene76516:CDS
CCAATAAAGCATACCTGACC+GGG 0.469066 2.3:+2578385 None:intergenic
AAGCTAGTGATATGAGAGCT+TGG 0.469144 2.3:+2579791 None:intergenic
ATAAATGGAACTCTTGAAGA+TGG 0.469340 2.3:+2579944 None:intergenic
TAGTTTGTTACCTTTCCAAA+TGG 0.469992 2.3:+2579120 None:intergenic
CTGAAGTACCTCCTCAATCA+TGG 0.473133 2.3:-2580337 MS.gene76516:CDS
TCTGTATTATTAGAAGCCAT+TGG 0.473376 2.3:-2578873 MS.gene76516:CDS
CTGGAGTTAATAAGAAGGCT+AGG 0.474420 2.3:-2579500 MS.gene76516:CDS
AGAGGTGGTTGGCTTGAAGA+TGG 0.480437 2.3:+2579471 None:intergenic
ATCCCTCCTCAATATATGCT+TGG 0.481586 2.3:-2580401 MS.gene76516:CDS
TCCAATAAAGCATACCTGAC+CGG 0.484473 2.3:+2578384 None:intergenic
TTCAAGCCAACCACCTCTAA+AGG 0.487455 2.3:-2579466 MS.gene76516:intron
GATGCATGCTCCACCAGTTA+GGG 0.504766 2.3:+2580438 None:intergenic
CCATTGTAGCAAATGATGGC+TGG 0.505228 2.3:-2580464 None:intergenic
GAGGAGTTTCGTGCTAACTC+TGG 0.506033 2.3:-2579857 MS.gene76516:CDS
TGGGCACCAGCATTTGGAAG+TGG 0.511663 2.3:-2577523 MS.gene76516:CDS
GGAGGGATATTCAAAGATGA+TGG 0.512004 2.3:+2580416 None:intergenic
TAGTGTAATAGCTCAACTAC+TGG 0.514825 2.3:-2579519 MS.gene76516:CDS
CCAAGTCTCTCTTCTTCACC+AGG 0.522523 2.3:+2580022 None:intergenic
ATTTCCTGAAGACCCCGGTC+AGG 0.529122 2.3:-2578398 MS.gene76516:intron
TAAAACCCACTTCCAAATGC+TGG 0.531421 2.3:+2577517 None:intergenic
AATTAACCTTTAGAGGTGGT+TGG 0.534058 2.3:+2579460 None:intergenic
TTTCTGTCAAGGAATTGAAA+GGG 0.538293 2.3:+2580366 None:intergenic
GTTTCTACAGAGTCAAATTG+AGG 0.544344 2.3:-2578841 MS.gene76516:intron
GAGTTAGCACGAAACTCCTC+AGG 0.546788 2.3:+2579860 None:intergenic
TTCCAAATGGAGAAACTAGC+TGG 0.547701 2.3:+2579133 None:intergenic
CATTGTAGCAAATGATGGCT+GGG 0.551321 2.3:-2580463 None:intergenic
GCTAGTTTCTCCATTTGGAA+AGG 0.554411 2.3:-2579130 MS.gene76516:intron
CCTGGTGAAGAAGAGAGACT+TGG 0.557044 2.3:-2580022 MS.gene76516:CDS
GGGCACCAGCATTTGGAAGT+GGG 0.558978 2.3:-2577522 MS.gene76516:CDS
TGAAAATGGAGCTGATTACT+GGG 0.559536 2.3:-2577542 MS.gene76516:CDS
TATAATAAACAGAATGCAGA+AGG 0.559856 2.3:-2577637 MS.gene76516:intron
AGTAGTTGACTCCATGATTG+AGG 0.561864 2.3:+2580326 None:intergenic
TGGAGTTAATAAGAAGGCTA+GGG 0.563568 2.3:-2579499 MS.gene76516:CDS
GAAGAGTAGAGGGTTGTGCT+TGG 0.563799 2.3:-2577596 MS.gene76516:CDS
CAAAGAACTTGAAGAGTAGA+GGG 0.566692 2.3:-2577606 MS.gene76516:CDS
ACATGTTGTGTGCATGACAC+TGG 0.568881 2.3:+2577571 None:intergenic
AACTACTGGAGTTAATAAGA+AGG 0.572553 2.3:-2579505 MS.gene76516:CDS
ATCTAGCTGTTGGATGACAC+TGG 0.583620 2.3:-2578091 MS.gene76516:intron
GAATCTGAATCAGGTGAGGA+AGG 0.588844 2.3:-2579187 MS.gene76516:intron
GAAGATCCAAGCATATATTG+AGG 0.591487 2.3:+2580395 None:intergenic
TAGAAACCTGATGTATCCAA+TGG 0.592817 2.3:+2578857 None:intergenic
ATGCTGCAGTGTCCAAGTAA+GGG 0.597172 2.3:+2578636 None:intergenic
ATAAAGCATGAAGTTTCTCA+AGG 0.600376 2.3:-2579914 MS.gene76516:CDS
GAGCGCTCCAATTCCAAACC+AGG 0.604751 2.3:-2578056 MS.gene76516:intron
GCTAGTGATATGAGAGCTTG+GGG 0.616346 2.3:+2579793 None:intergenic
GATCCAAGCATATATTGAGG+AGG 0.624438 2.3:+2580398 None:intergenic
ATCCAAGCATATATTGAGGA+GGG 0.625532 2.3:+2580399 None:intergenic
CAGGTGAGGAAGGAGAAGCT+AGG 0.634871 2.3:-2579177 MS.gene76516:CDS
TCTGTATTTCCTGAAGACCC+CGG 0.639899 2.3:-2578403 MS.gene76516:CDS
GAGTAATTAACCTTTAGAGG+TGG 0.639994 2.3:+2579456 None:intergenic
GTGTCATGCACACAACATGT+TGG 0.662430 2.3:-2577568 MS.gene76516:CDS
TGCTGCAGTGTCCAAGTAAG+GGG 0.663325 2.3:+2578637 None:intergenic
ACTACCAGATCAAATATCTG+AGG 0.665989 2.3:-2579711 MS.gene76516:intron
AGTTGACTCCATGATTGAGG+AGG 0.689840 2.3:+2580329 None:intergenic
TCAACAAGGGCATCATCCTG+AGG 0.692914 2.3:-2579876 MS.gene76516:CDS
ATTGGATTTCACTTACAACA+AGG 0.702031 2.3:-2579753 MS.gene76516:CDS
TGGCTGGGAACCCTAACTGG+TGG 0.716766 2.3:-2580448 MS.gene76516:CDS
CAATAAAGCATACCTGACCG+GGG 0.742945 2.3:+2578386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAATAAAATTAAAAA+AGG + chr2.3:2579513-2579532 None:intergenic 0.0%
!! AAAATAAAATTAAAAAAGGA+AGG + chr2.3:2579509-2579528 None:intergenic 10.0%
!! AATATAGTATTATTATTGAA+GGG - chr2.3:2579473-2579492 MS.gene76516:CDS 10.0%
!! ATATATATATATATATAGGA+GGG + chr2.3:2580009-2580028 None:intergenic 10.0%
!! TATATATATATATATATAGG+AGG + chr2.3:2580010-2580029 None:intergenic 10.0%
!!! TTTTATAAAAAACTATTAAC+AGG - chr2.3:2579525-2579544 MS.gene76516:intron 10.0%
!!! TTTTTTTGTTTTTTTTTTGA+AGG - chr2.3:2578307-2578326 MS.gene76516:intron 10.0%
!! AATGAATTCTAATTAATTGA+TGG + chr2.3:2579611-2579630 None:intergenic 15.0%
!! AATTCATTAGAATCAAATTA+AGG - chr2.3:2579623-2579642 MS.gene76516:intron 15.0%
!! ATTCATTAGAATCAAATTAA+GGG - chr2.3:2579624-2579643 MS.gene76516:intron 15.0%
!! GAATATAGTATTATTATTGA+AGG - chr2.3:2579472-2579491 MS.gene76516:CDS 15.0%
!! TAATTTCTATCTTAATTACT+TGG - chr2.3:2578572-2578591 MS.gene76516:intron 15.0%
!! TATATATATATATATAGGAG+GGG + chr2.3:2580008-2580027 None:intergenic 15.0%
!!! AATGTCACTTTAATAAATAA+TGG - chr2.3:2580306-2580325 MS.gene76516:intron 15.0%
!!! TTTTCTTCATTATAATTACA+TGG - chr2.3:2577879-2577898 MS.gene76516:intron 15.0%
!!! TTTTTTTTTGAAGGATATAT+AGG - chr2.3:2578316-2578335 MS.gene76516:intron 15.0%
!! AAACATTCTTTCTTTAGTTA+TGG - chr2.3:2579263-2579282 MS.gene76516:intron 20.0%
!! AAATTCTTATCCTTAAGTAT+AGG - chr2.3:2578734-2578753 MS.gene76516:intron 20.0%
!! AACATTCTTTCTTTAGTTAT+GGG - chr2.3:2579264-2579283 MS.gene76516:intron 20.0%
!! ACAATGGTTTATGATAATTA+TGG + chr2.3:2578884-2578903 None:intergenic 20.0%
!! CTTAAGTAGTGATAATATAT+TGG - chr2.3:2578197-2578216 MS.gene76516:intron 20.0%
!! GAAATTATAGAAAAGAAAAG+AGG + chr2.3:2578559-2578578 None:intergenic 20.0%
!! GAAGAAAAAAAACATGAATA+AGG + chr2.3:2577867-2577886 None:intergenic 20.0%
!! GTAAATTATAGGTCATATAT+AGG - chr2.3:2578279-2578298 MS.gene76516:intron 20.0%
!!! AAATATCTGAGGTAAATTAT+AGG - chr2.3:2578268-2578287 MS.gene76516:intron 20.0%
!!! CATTGAAATTTGAAATAACA+AGG - chr2.3:2579730-2579749 MS.gene76516:CDS 20.0%
!!! CTTGTTATTTCAAATTTCAA+TGG + chr2.3:2579732-2579751 None:intergenic 20.0%
!!! TAATTTTGAACCTATACTTA+AGG + chr2.3:2578747-2578766 None:intergenic 20.0%
!!! TGCTTTATTATATCCATAAA+TGG + chr2.3:2578042-2578061 None:intergenic 20.0%
! AAAAGAAAGAAAAGAATACC+TGG + chr2.3:2579933-2579952 None:intergenic 25.0%
! AACCAAATTAACACAAACAA+TGG + chr2.3:2578900-2578919 None:intergenic 25.0%
! AACCATTGTTTGTGTTAATT+TGG - chr2.3:2578895-2578914 MS.gene76516:intron 25.0%
! ATACTAAAATCATGATTCCA+TGG + chr2.3:2579971-2579990 None:intergenic 25.0%
! ATTATATGTGAATCTGAATC+AGG - chr2.3:2578772-2578791 MS.gene76516:intron 25.0%
! CAAAAAGAATATTACAGTGT+TGG + chr2.3:2578663-2578682 None:intergenic 25.0%
! GAAATTGAATAGGACAAAAA+TGG - chr2.3:2579419-2579438 MS.gene76516:intron 25.0%
! TACTAAAATCATGATTCCAT+GGG + chr2.3:2579970-2579989 None:intergenic 25.0%
! TATAATAAACAGAATGCAGA+AGG - chr2.3:2580331-2580350 MS.gene76516:CDS 25.0%
! TATCATCAAGTGCATAAATT+GGG - chr2.3:2580141-2580160 MS.gene76516:intron 25.0%
! TTCAATCAATGACAAAAGAT+AGG + chr2.3:2578998-2579017 None:intergenic 25.0%
!! AAGAAAGAAAGATGAGTTTT+TGG - chr2.3:2579044-2579063 MS.gene76516:intron 25.0%
!! AGAATTTTTATGGTCAACAA+GGG - chr2.3:2578079-2578098 MS.gene76516:CDS 25.0%
!!! ACTTTTCTCTGAAATTGAAT+AGG - chr2.3:2579409-2579428 MS.gene76516:intron 25.0%
AAAGAAAAGAATACCTGGTT+TGG + chr2.3:2579928-2579947 None:intergenic 30.0%
AAAGATAGGTACACAGAAAT+TGG + chr2.3:2578984-2579003 None:intergenic 30.0%
AACAACTACTATTTCATGTG+TGG + chr2.3:2578935-2578954 None:intergenic 30.0%
AACCTAAAAACTCAGAAAAC+TGG - chr2.3:2577983-2578002 MS.gene76516:intron 30.0%
AACTACTGGAGTTAATAAGA+AGG - chr2.3:2578463-2578482 MS.gene76516:intron 30.0%
ACCTAAAAACTCAGAAAACT+GGG - chr2.3:2577984-2578003 MS.gene76516:intron 30.0%
ACTAAAATCATGATTCCATG+GGG + chr2.3:2579969-2579988 None:intergenic 30.0%
ATAAAGCATGAAGTTTCTCA+AGG - chr2.3:2578054-2578073 MS.gene76516:intron 30.0%
ATAAATGGAACTCTTGAAGA+TGG + chr2.3:2578027-2578046 None:intergenic 30.0%
ATCTTCAAGAGTTCCATTTA+TGG - chr2.3:2578026-2578045 MS.gene76516:intron 30.0%
ATTGGATTTCACTTACAACA+AGG - chr2.3:2578215-2578234 MS.gene76516:intron 30.0%
GTAGTAATAACTGTAAAGAG+AGG + chr2.3:2579657-2579676 None:intergenic 30.0%
GTATCATCAAGTGCATAAAT+TGG - chr2.3:2580140-2580159 MS.gene76516:intron 30.0%
TCAGAGAAAAGTGAAACATT+GGG + chr2.3:2579401-2579420 None:intergenic 30.0%
TCTGTATTATTAGAAGCCAT+TGG - chr2.3:2579095-2579114 MS.gene76516:intron 30.0%
TGTGAGTAATTAACCTTTAG+AGG + chr2.3:2578518-2578537 None:intergenic 30.0%
TTCAGAGAAAAGTGAAACAT+TGG + chr2.3:2579402-2579421 None:intergenic 30.0%
TTTACCTCAGATATTTGATC+TGG + chr2.3:2578264-2578283 None:intergenic 30.0%
TTTCTGTCAAGGAATTGAAA+GGG + chr2.3:2577605-2577624 None:intergenic 30.0%
! ACATGGTGTGAAAATTTTGA+GGG - chr2.3:2577896-2577915 MS.gene76516:intron 30.0%
! ATGCACTTGATGATACTATA+TGG + chr2.3:2580136-2580155 None:intergenic 30.0%
! CTACAGTATAGAAGATTTTG+AGG + chr2.3:2577796-2577815 None:intergenic 30.0%
! GAGAATTTTTATGGTCAACA+AGG - chr2.3:2578078-2578097 MS.gene76516:CDS 30.0%
! GCAACTGTGATTTAAAATTG+TGG + chr2.3:2577751-2577770 None:intergenic 30.0%
! GTGATTTAAAATTGTGGTGA+TGG + chr2.3:2577745-2577764 None:intergenic 30.0%
! TACATGGTGTGAAAATTTTG+AGG - chr2.3:2577895-2577914 MS.gene76516:intron 30.0%
! TCTCAAGGTGAGAATTTTTA+TGG - chr2.3:2578069-2578088 MS.gene76516:CDS 30.0%
! TGAATTTTCTGTGAGTTGTT+AGG - chr2.3:2580232-2580251 MS.gene76516:intron 30.0%
! TTCAGCATTATTTTCTGTCA+AGG + chr2.3:2577616-2577635 None:intergenic 30.0%
! TTTTCTGTCAAGGAATTGAA+AGG + chr2.3:2577606-2577625 None:intergenic 30.0%
! TTTTGTATGTTTTGCAGTAC+TGG - chr2.3:2577919-2577938 MS.gene76516:intron 30.0%
!! TAGTTTGTTACCTTTCCAAA+TGG + chr2.3:2578851-2578870 None:intergenic 30.0%
ACAGAATGCAGAAGGTAATA+AGG - chr2.3:2580339-2580358 MS.gene76516:CDS 35.0%
ACTACCAGATCAAATATCTG+AGG - chr2.3:2578257-2578276 MS.gene76516:intron 35.0%
AGCATTTGAACACATGTAGT+AGG + chr2.3:2580280-2580299 None:intergenic 35.0%
ATCCAAGCATATATTGAGGA+GGG + chr2.3:2577572-2577591 None:intergenic 35.0%
CAAAGAACTTGAAGAGTAGA+GGG - chr2.3:2580362-2580381 MS.gene76516:CDS 35.0%
CAACATGTTGGAAGTGAAAA+TGG - chr2.3:2580412-2580431 MS.gene76516:CDS 35.0%
CAGCATCAAAAAACATGATG+AGG - chr2.3:2579349-2579368 MS.gene76516:intron 35.0%
GAAGATCCAAGCATATATTG+AGG + chr2.3:2577576-2577595 None:intergenic 35.0%
GTTTCTACAGAGTCAAATTG+AGG - chr2.3:2579127-2579146 MS.gene76516:intron 35.0%
TAGAAACCTGATGTATCCAA+TGG + chr2.3:2579114-2579133 None:intergenic 35.0%
TAGTGTAATAGCTCAACTAC+TGG - chr2.3:2578449-2578468 MS.gene76516:intron 35.0%
TGAAAATGGAGCTGATTACT+GGG - chr2.3:2580426-2580445 MS.gene76516:CDS 35.0%
TGGAGTTAATAAGAAGGCTA+GGG - chr2.3:2578469-2578488 MS.gene76516:intron 35.0%
! AATTAACCTTTAGAGGTGGT+TGG + chr2.3:2578511-2578530 None:intergenic 35.0%
! GAGTAATTAACCTTTAGAGG+TGG + chr2.3:2578515-2578534 None:intergenic 35.0%
! TCCCAGTTTTCTGAGTTTTT+AGG + chr2.3:2577988-2578007 None:intergenic 35.0%
! TTATTCCTCTCTTTTGTTGC+AGG - chr2.3:2579299-2579318 MS.gene76516:intron 35.0%
! TTTTATTCCCATCTAGCTGT+TGG - chr2.3:2579867-2579886 MS.gene76516:CDS 35.0%
!! AAGAATACCTGGTTTGGAAT+TGG + chr2.3:2579922-2579941 None:intergenic 35.0%
!!! CAAGCTACGGTTTTAAATTG+CGG + chr2.3:2577826-2577845 None:intergenic 35.0%
AAGCTAGTGATATGAGAGCT+TGG + chr2.3:2578180-2578199 None:intergenic 40.0%
AGCTAGTGATATGAGAGCTT+GGG + chr2.3:2578179-2578198 None:intergenic 40.0%
ATCCCTCCTCAATATATGCT+TGG - chr2.3:2577567-2577586 MS.gene76516:CDS 40.0%
ATGTGAATCTGAATCAGGTG+AGG - chr2.3:2578777-2578796 MS.gene76516:intron 40.0%
CTGGAGTTAATAAGAAGGCT+AGG - chr2.3:2578468-2578487 MS.gene76516:intron 40.0%
GATCCAAGCATATATTGAGG+AGG + chr2.3:2577573-2577592 None:intergenic 40.0%
GATGCTGTAACTTCAAGCTA+CGG + chr2.3:2577839-2577858 None:intergenic 40.0%
GCAAAGAACTTGAAGAGTAG+AGG - chr2.3:2580361-2580380 MS.gene76516:CDS 40.0%
GGAGGGATATTCAAAGATGA+TGG + chr2.3:2577555-2577574 None:intergenic 40.0%
GTGAAAATGGAGCTGATTAC+TGG - chr2.3:2580425-2580444 MS.gene76516:CDS 40.0%
TAAAACCCACTTCCAAATGC+TGG + chr2.3:2580454-2580473 None:intergenic 40.0%
TAGAAGCCATTGGATACATC+AGG - chr2.3:2579105-2579124 MS.gene76516:intron 40.0%
TCCAATAAAGCATACCTGAC+CGG + chr2.3:2579587-2579606 None:intergenic 40.0%
TCTGTGAGTTGTTAGGTCAT+TGG - chr2.3:2580239-2580258 MS.gene76516:intron 40.0%
TGGTTCATCTTGGTCACATA+TGG - chr2.3:2578140-2578159 MS.gene76516:intron 40.0%
TTCCAAATGGAGAAACTAGC+TGG + chr2.3:2578838-2578857 None:intergenic 40.0%
! AGTAGTTGACTCCATGATTG+AGG + chr2.3:2577645-2577664 None:intergenic 40.0%
! GCTAGTTTCTCCATTTGGAA+AGG - chr2.3:2578838-2578857 MS.gene76516:intron 40.0%
!! TTGGAGCGCTCTTTCTTTTT+AGG + chr2.3:2579903-2579922 None:intergenic 40.0%
ACATGTTGTGTGCATGACAC+TGG + chr2.3:2580400-2580419 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGTA+TGG + chr2.3:2577681-2577700 None:intergenic 45.0%
AGTGTCATCCAACAGCTAGA+TGG + chr2.3:2579878-2579897 None:intergenic 45.0%
ATCTAGCTGTTGGATGACAC+TGG - chr2.3:2579877-2579896 MS.gene76516:CDS 45.0%
ATGCTGCAGTGTCCAAGTAA+GGG + chr2.3:2579335-2579354 None:intergenic 45.0%
CAATAAAGCATACCTGACCG+GGG + chr2.3:2579585-2579604 None:intergenic 45.0%
CCAATAAAGCATACCTGACC+GGG + chr2.3:2579586-2579605 None:intergenic 45.0%
CTGGATCACTTGGTTCATCT+TGG - chr2.3:2578130-2578149 MS.gene76516:intron 45.0%
GAATCTGAATCAGGTGAGGA+AGG - chr2.3:2578781-2578800 MS.gene76516:intron 45.0%
GCTAGTGATATGAGAGCTTG+GGG + chr2.3:2578178-2578197 None:intergenic 45.0%
GTGTCATCCAACAGCTAGAT+GGG + chr2.3:2579877-2579896 None:intergenic 45.0%
GTGTCATGCACACAACATGT+TGG - chr2.3:2580400-2580419 MS.gene76516:CDS 45.0%
TCTGTATTTCCTGAAGACCC+CGG - chr2.3:2579565-2579584 MS.gene76516:intron 45.0%
TTCAAGCCAACCACCTCTAA+AGG - chr2.3:2578502-2578521 MS.gene76516:intron 45.0%
TTTCTACAAAAGCTGCCCCA+TGG - chr2.3:2579951-2579970 MS.gene76516:CDS 45.0%
! AGTTGACTCCATGATTGAGG+AGG + chr2.3:2577642-2577661 None:intergenic 45.0%
! CACCAGCTAGTTTCTCCATT+TGG - chr2.3:2578833-2578852 MS.gene76516:intron 45.0%
! CTGAAGTACCTCCTCAATCA+TGG - chr2.3:2577631-2577650 MS.gene76516:intron 45.0%
!! TTTTGCAGTACTGGACTACC+TGG - chr2.3:2577928-2577947 MS.gene76516:intron 45.0%
!! ATAAATAAATAAGAATAATA+TGG + chr2.3:2579161-2579180 None:intergenic 5.0%
!! CAATATATATATATATATAT+AGG + chr2.3:2580013-2580032 None:intergenic 5.0%
CCAAGTCTCTCTTCTTCACC+AGG + chr2.3:2577949-2577968 None:intergenic 50.0%
CCCGGTCAGGTATGCTTTAT+TGG - chr2.3:2579583-2579602 MS.gene76516:intron 50.0%
CCTGGTGAAGAAGAGAGACT+TGG - chr2.3:2577946-2577965 MS.gene76516:intron 50.0%
CGTGCTAACTCTGGATCACT+TGG - chr2.3:2578120-2578139 MS.gene76516:intron 50.0%
GAAGAGTAGAGGGTTGTGCT+TGG - chr2.3:2580372-2580391 MS.gene76516:CDS 50.0%
GAGGAGTTTCGTGCTAACTC+TGG - chr2.3:2578111-2578130 MS.gene76516:intron 50.0%
GAGTGCCTGCAACAAAAGAG+AGG + chr2.3:2579307-2579326 None:intergenic 50.0%
GAGTTAGCACGAAACTCCTC+AGG + chr2.3:2578111-2578130 None:intergenic 50.0%
GATGCATGCTCCACCAGTTA+GGG + chr2.3:2577533-2577552 None:intergenic 50.0%
GATGCTGCAGTGTCCAAGTA+AGG + chr2.3:2579336-2579355 None:intergenic 50.0%
GATTACTGGGCACCAGCATT+TGG - chr2.3:2580439-2580458 MS.gene76516:CDS 50.0%
TGAGAGCTTGGGGAAGAAAC+TGG + chr2.3:2578168-2578187 None:intergenic 50.0%
TGCTGCAGTGTCCAAGTAAG+GGG + chr2.3:2579334-2579353 None:intergenic 50.0%
!! AGAGGTGGTTGGCTTGAAGA+TGG + chr2.3:2578500-2578519 None:intergenic 50.0%
!! TCAACAAGGGCATCATCCTG+AGG - chr2.3:2578092-2578111 MS.gene76516:intron 50.0%
ATTTCCTGAAGACCCCGGTC+AGG - chr2.3:2579570-2579589 MS.gene76516:intron 55.0%
CAGGTGAGGAAGGAGAAGCT+AGG - chr2.3:2578791-2578810 MS.gene76516:intron 55.0%
GAGCGCTCCAATTCCAAACC+AGG - chr2.3:2579912-2579931 MS.gene76516:CDS 55.0%
GGATGCATGCTCCACCAGTT+AGG + chr2.3:2577534-2577553 None:intergenic 55.0%
GGGCACCAGCATTTGGAAGT+GGG - chr2.3:2580446-2580465 MS.gene76516:CDS 55.0%
TGATGGCTGGGAACCCTAAC+TGG - chr2.3:2577517-2577536 MS.gene76516:CDS 55.0%
TGGGCACCAGCATTTGGAAG+TGG - chr2.3:2580445-2580464 MS.gene76516:CDS 55.0%
CATACCTGACCGGGGTCTTC+AGG + chr2.3:2579577-2579596 None:intergenic 60.0%
GGCACTCAGCACCCCTTACT+TGG - chr2.3:2579320-2579339 MS.gene76516:intron 60.0%
TGGCTGGGAACCCTAACTGG+TGG - chr2.3:2577520-2577539 MS.gene76516:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 2577516 2580474 2577516 ID=MS.gene76516
chr2.3 mRNA 2577516 2580474 2577516 ID=MS.gene76516.t1;Parent=MS.gene76516
chr2.3 exon 2580323 2580474 2580323 ID=MS.gene76516.t1.exon1;Parent=MS.gene76516.t1
chr2.3 CDS 2580323 2580474 2580323 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2579712 2580054 2579712 ID=MS.gene76516.t1.exon2;Parent=MS.gene76516.t1
chr2.3 CDS 2579712 2580054 2579712 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2579467 2579538 2579467 ID=MS.gene76516.t1.exon3;Parent=MS.gene76516.t1
chr2.3 CDS 2579467 2579538 2579467 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2579131 2579196 2579131 ID=MS.gene76516.t1.exon4;Parent=MS.gene76516.t1
chr2.3 CDS 2579131 2579196 2579131 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2578842 2578907 2578842 ID=MS.gene76516.t1.exon5;Parent=MS.gene76516.t1
chr2.3 CDS 2578842 2578907 2578842 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2578607 2578669 2578607 ID=MS.gene76516.t1.exon6;Parent=MS.gene76516.t1
chr2.3 CDS 2578607 2578669 2578607 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2578399 2578443 2578399 ID=MS.gene76516.t1.exon7;Parent=MS.gene76516.t1
chr2.3 CDS 2578399 2578443 2578399 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2578057 2578107 2578057 ID=MS.gene76516.t1.exon8;Parent=MS.gene76516.t1
chr2.3 CDS 2578057 2578107 2578057 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
chr2.3 exon 2577516 2577647 2577516 ID=MS.gene76516.t1.exon9;Parent=MS.gene76516.t1
chr2.3 CDS 2577516 2577647 2577516 ID=cds.MS.gene76516.t1;Parent=MS.gene76516.t1
Gene Sequence

>MS.gene76516

ATGATGGCTGGGAACCCTAACTGGTGGAGCATGCATCCATCATCTTTGAATATCCCTCCTCAATATATGCTTGGATCTTCTTCAATCCCTTTCAATTCCTTGACAGAAAATAATGCTGAAGTACCTCCTCAATCATGGAGTCAACTACTTTTGTTAGTAAAACCATACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGCTTCTTAATTTGTGTCCATCACCACAATTTTAAATCACAGTTGCAGTTAAGCTACAGTTACAGTGACCTCAAAATCTTCTATACTGTAGTTGCAGACCGCAATTTAAAACCGTAGCTTGAAGTTACAGCATCTTTGACCTTATTCATGTTTTTTTTCTTCATTATAATTACATGGTGTGAAAATTTTGAGGGTTTTGTATGTTTTGCAGTACTGGACTACCTGGTGAAGAAGAGAGACTTGGTTTTAATCATTTTCAACCTAAAAACTCAGAAAACTGGGATGTGCAAATTATGAATCCATCTTCAAGAGTTCCATTTATGGATATAATAAAGCATGAAGTTTCTCAAGGTGAGAATTTTTATGGTCAACAAGGGCATCATCCTGAGGAGTTTCGTGCTAACTCTGGATCACTTGGTTCATCTTGGTCACATATGGTGCCAGTTTCTTCCCCAAGCTCTCATATCACTAGCTTAAGTAGTGATAATATATTGGATTTCACTTACAACAAGGTAGATCATAGCAAGAATCAACTACCAGATCAAATATCTGAGGTAAATTATAGGTCATATATAGGTTCTTTTTTTTTGTTTTTTTTTTGAAGGATATATAGGTTCATGTTAATTATTATTTTTATCAACTTCTAATATTGTTATTTATTAATATAAGTTATGTATATATGATATTGAAATTAATTTCATCTATATCTTTCATATATATGCTATAGTGTAATAGCTCAACTACTGGAGTTAATAAGAAGGCTAGGGTTCAGCCATCTTCAAGCCAACCACCTCTAAAGGTTAATTACTCACAACAAATTAACACTTTTAACCTCTTTTCTTTTCTATAATTTCTATCTTAATTACTTGGTAATTAGTGAGCTTGTATATATGTGTATGTTTTGTTGTTATTGTTTTTCTTGATTGTCTTTCTCTCCAACACTGTAATATTCTTTTTGCTGATATTCATATATATACTTGTTGAATGAGAGAAATTATTTAAGCATATGAAATTCTTATCCTTAAGTATAGGTTCAAAATTATACTAATTATATGTGAATCTGAATCAGGTGAGGAAGGAGAAGCTAGGTGATAGAATAACAGCACTTCACCAGCTAGTTTCTCCATTTGGAAAGGTAACAAACTAATTAATGACTCCATAATTATCATAAACCATTGTTTGTGTTAATTTGGTTTTATAAAAAAGTCCACACATGAAATAGTAGTTGTTTTCAATTCAAAGTAGACTTTGATGATCCAATTTCTGTGTACCTATCTTTTGTCATTGATTGAAATTTCACTTTTAACATTAAAAGAAGAAAGAAAGAAAGATGAGTTTTTGGTGCTTCACTTTTGCAGACTGACACAGCTTCTGTATTATTAGAAGCCATTGGATACATCAGGTTTCTACAGAGTCAAATTGAGGTGATGTTTCCATATTATTCTTATTTATTTATTAATTATACTATGCAGCAAGTTAGTATTTCAGAACATGCACTTAAAGTTTGTTCAATTTTGATGTGATTTTTTTCACTCTAAAAACATTCTTTCTTTAGTTATGGGAGTTATAAAATTTTATTCCTCTCTTTTGTTGCAGGCACTCAGCACCCCTTACTTGGACACTGCAGCATCAAAAAACATGATGAGGAATCAACATTCTGTAAGTTCACATTTCCCAATGTTTCACTTTTCTCTGAAATTGAATAGGACAAAAATGGAGTATACTTAATGTATTTGTTAGAATTATAGAATATAGTATTATTATTGAAGGGAATGTTTGTTCCTTCCTTTTTTAATTTTATTTTATAAAAAACTATTAACAGGTGCATGTTGAAAGAAATTCTGTATTTCCTGAAGACCCCGGTCAGGTATGCTTTATTGGATCCATCAATTAATTAGAATTCATTAGAATCAAATTAAGGGAAATTAACCTCTCTTTACAGTTATTACTACACTATTTAATTAATTAATTAATTAATTAATATTAATATGCTCAAGAAAGAAGCCATTGAAATTTGAAATAACAAGGTTTCTCAGCTTTTTATATAACATCTTTTAGAAAAAGCATGATGAAGCTGAATTGTTCACTTGAAGTCAAATTTCATATAGAAGTGAAATTAGTAAATAATATATACTTTCTTCTTTTTATTCCCATCTAGCTGTTGGATGACACTGGCCTAAAAAGAAAGAGCGCTCCAATTCCAAACCAGGTATTCTTTTCTTTCTTTTTCTACAAAAGCTGCCCCATGGAATCATGATTTTAGTATTCTTCTTCATTTGACCCCTCCTATATATATATATATATATTGAAGAAATATATGTGTGTATCTTATATTTATATATAAGATTCTAATCACTCTAATAAATATTATTAAAAATAAATAATACTGTACTATATTATCATTATAACCATATAGTATCATCAAGTGCATAAATTGGGCAATGAAACTGTCAGACTTTGCATATGATTCTTATTGTTTAATTAATTAATAAAAAATAATAATGATGTGAATTTTCTGTGAGTTGTTAGGTCATTGGCAAGAGTTTCATGAACCTACTACATGTGTTCAAATGCTTAATGTAATGTCACTTTAATAAATAATGGTTTATAATAAACAGAATGCAGAAGGTAATAAGGCAAAGAACTTGAAGAGTAGAGGGTTGTGCTTGGTTCCAGTGTCATGCACACAACATGTTGGAAGTGAAAATGGAGCTGATTACTGGGCACCAGCATTTGGAAGTGGGTTTTAA
Protein sequence

>MS.gene76516.t1

MMAGNPNWWSMHPSSLNIPPQYMLGSSSIPFNSLTENNAEVPPQSWSQLLFTGLPGEEERLGFNHFQPKNSENWDVQIMNPSSRVPFMDIIKHEVSQGENFYGQQGHHPEEFRANSGSLGSSWSHMVPVSSPSSHITSLSSDNILDFTYNKVDHSKNQLPDQISECNSSTTGVNKKARVQPSSSQPPLKVRKEKLGDRITALHQLVSPFGKTDTASVLLEAIGYIRFLQSQIEALSTPYLDTAASKNMMRNQHSVHVERNSVFPEDPGQLLDDTGLKRKSAPIPNQNAEGNKAKNLKSRGLCLVPVSCTQHVGSENGADYWAPAFGSGF