Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76677.t1 | KEH29169.1 | 94.2 | 173 | 10 | 0 | 13 | 185 | 60 | 232 | 5.20E-84 | 320.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76677.t1 | A0A072UHD2 | 94.2 | 173 | 10 | 0 | 13 | 185 | 60 | 232 | 3.8e-84 | 320.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene76677 | 0.855148 | 7.66E-62 | -1.69E-46 |
| MS.gene051131 | MS.gene76677 | 0.814077 | 1.85E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene76677 | 0.82082 | 5.60E-53 | -1.69E-46 |
| MS.gene054146 | MS.gene76677 | 0.824236 | 9.01E-54 | -1.69E-46 |
| MS.gene059265 | MS.gene76677 | 0.900584 | 6.33E-78 | -1.69E-46 |
| MS.gene059955 | MS.gene76677 | 0.829728 | 4.38E-55 | -1.69E-46 |
| MS.gene061092 | MS.gene76677 | 0.800739 | 1.25E-48 | -1.69E-46 |
| MS.gene061094 | MS.gene76677 | 0.807887 | 4.06E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76677.t1 | MTR_4g027195 | 94.220 | 173 | 10 | 0 | 13 | 185 | 60 | 232 | 2.49e-110 | 314 |
| MS.gene76677.t1 | MTR_4g027105 | 68.421 | 171 | 48 | 3 | 13 | 181 | 25 | 191 | 1.44e-70 | 212 |
| MS.gene76677.t1 | MTR_4g027225 | 66.667 | 171 | 51 | 3 | 13 | 181 | 25 | 191 | 6.16e-59 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGCCCTTCTTCTCTT+TGG | 0.204053 | 4.1:-71903795 | MS.gene76677:CDS |
| TCAACTAGGTCAGCTGGATT+TGG | 0.237066 | 4.1:+71903555 | None:intergenic |
| TGGGTTGTGCTCTTCGTATT+GGG | 0.288688 | 4.1:-71903720 | MS.gene76677:CDS |
| TTGGGTTGTGCTCTTCGTAT+TGG | 0.299686 | 4.1:-71903721 | MS.gene76677:CDS |
| TCGTCACTCGCTTAAAGTTT+TGG | 0.323243 | 4.1:-71903676 | MS.gene76677:CDS |
| TAGATTACCAACTACTTTGA+AGG | 0.357772 | 4.1:-71904042 | MS.gene76677:CDS |
| TCTCTTTGGAATACTTTCTT+CGG | 0.374925 | 4.1:-71903781 | MS.gene76677:CDS |
| CAACTAGGTCAGCTGGATTT+GGG | 0.375194 | 4.1:+71903556 | None:intergenic |
| TGCATCTACTGCTCGCTAAT+CGG | 0.377217 | 4.1:-71903867 | MS.gene76677:CDS |
| AAAGTATTCCAAAGAGAAGA+AGG | 0.428632 | 4.1:+71903787 | None:intergenic |
| GGTGTTGATATTCAATACTA+AGG | 0.429220 | 4.1:-71903610 | MS.gene76677:CDS |
| TCTTGATCCTTCAAAGTAGT+TGG | 0.429294 | 4.1:+71904035 | None:intergenic |
| GAGTGACGAGCAAGTATAAC+TGG | 0.443034 | 4.1:+71903690 | None:intergenic |
| ATGGCATGGCATGGGATTGA+AGG | 0.461512 | 4.1:+71904005 | None:intergenic |
| CTACCTTCAACTAGGTCAGC+TGG | 0.475286 | 4.1:+71903549 | None:intergenic |
| AATCCAGCTGACCTAGTTGA+AGG | 0.483463 | 4.1:-71903552 | MS.gene76677:CDS |
| TTTCTTGACACAATACTCAT+TGG | 0.492166 | 4.1:-71903975 | MS.gene76677:CDS |
| ATGAACTTTGTGATCATCAA+AGG | 0.497786 | 4.1:+71903914 | None:intergenic |
| AACACAAAGATGGCATGGCA+TGG | 0.520422 | 4.1:+71903996 | None:intergenic |
| ACACAAAGATGGCATGGCAT+GGG | 0.550895 | 4.1:+71903997 | None:intergenic |
| CGGCAGCAAGATGGAGAGAA+GGG | 0.553496 | 4.1:+71903755 | None:intergenic |
| AAGTTGTTGCGGCAGCAAGA+TGG | 0.558737 | 4.1:+71903746 | None:intergenic |
| TGAACTTTGTGATCATCAAA+GGG | 0.562970 | 4.1:+71903915 | None:intergenic |
| GATGCAGATGGATGTCAAGA+AGG | 0.564412 | 4.1:+71903884 | None:intergenic |
| ATTAAGATCTACCTTCAACT+AGG | 0.573127 | 4.1:+71903541 | None:intergenic |
| TGGCATGGCATGGGATTGAA+GGG | 0.575309 | 4.1:+71904006 | None:intergenic |
| TAGGTCAGCTGGATTTGGGT+AGG | 0.578541 | 4.1:+71903560 | None:intergenic |
| TGTGTCAAGAAACACAAAGA+TGG | 0.584873 | 4.1:+71903986 | None:intergenic |
| GCGGCAGCAAGATGGAGAGA+AGG | 0.589371 | 4.1:+71903754 | None:intergenic |
| CAAGAAACACAAAGATGGCA+TGG | 0.592259 | 4.1:+71903991 | None:intergenic |
| AAGTATTCCAAAGAGAAGAA+GGG | 0.599617 | 4.1:+71903788 | None:intergenic |
| GTGTCGGATAATAGAAGCGA+TGG | 0.602232 | 4.1:-71903652 | MS.gene76677:CDS |
| TAGCGAGCAGTAGATGCAGA+TGG | 0.605364 | 4.1:+71903872 | None:intergenic |
| GGTAGTAAAGATTACTACCA+TGG | 0.620964 | 4.1:-71903631 | MS.gene76677:CDS |
| AATACTAAGGATTCAGCCAG+TGG | 0.626440 | 4.1:-71903597 | MS.gene76677:CDS |
| AAGATCTACACGAAGACTCG+TGG | 0.646014 | 4.1:-71903840 | MS.gene76677:CDS |
| AGTATTGAATATCAACACCA+TGG | 0.687774 | 4.1:+71903614 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATGTTTTATATATCTAAT+CGG | - | chr4.1:71903548-71903567 | MS.gene76677:CDS | 10.0% |
| !!! | CAAAGCTAAGAATTTTTTCT+TGG | + | chr4.1:71903811-71903830 | None:intergenic | 25.0% |
| AAGTATTCCAAAGAGAAGAA+GGG | + | chr4.1:71903834-71903853 | None:intergenic | 30.0% | |
| ATGAACTTTGTGATCATCAA+AGG | + | chr4.1:71903708-71903727 | None:intergenic | 30.0% | |
| TAGATTACCAACTACTTTGA+AGG | - | chr4.1:71903577-71903596 | MS.gene76677:CDS | 30.0% | |
| TGAACTTTGTGATCATCAAA+GGG | + | chr4.1:71903707-71903726 | None:intergenic | 30.0% | |
| TTTCTTGACACAATACTCAT+TGG | - | chr4.1:71903644-71903663 | MS.gene76677:CDS | 30.0% | |
| ! | AAAGTATTCCAAAGAGAAGA+AGG | + | chr4.1:71903835-71903854 | None:intergenic | 30.0% |
| ! | AGTATTGAATATCAACACCA+TGG | + | chr4.1:71904008-71904027 | None:intergenic | 30.0% |
| !! | GGTGTTGATATTCAATACTA+AGG | - | chr4.1:71904009-71904028 | MS.gene76677:CDS | 30.0% |
| !! | TCTCTTTGGAATACTTTCTT+CGG | - | chr4.1:71903838-71903857 | MS.gene76677:CDS | 30.0% |
| !!! | AGCTAAGAATTTTTTCTTGG+TGG | + | chr4.1:71903808-71903827 | None:intergenic | 30.0% |
| ACAACCCAAAAAAGTTGTTG+CGG | + | chr4.1:71903887-71903906 | None:intergenic | 35.0% | |
| GGTAGTAAAGATTACTACCA+TGG | - | chr4.1:71903988-71904007 | MS.gene76677:CDS | 35.0% | |
| TCTTGATCCTTCAAAGTAGT+TGG | + | chr4.1:71903587-71903606 | None:intergenic | 35.0% | |
| TGTGTCAAGAAACACAAAGA+TGG | + | chr4.1:71903636-71903655 | None:intergenic | 35.0% | |
| AATACTAAGGATTCAGCCAG+TGG | - | chr4.1:71904022-71904041 | MS.gene76677:CDS | 40.0% | |
| CAAGAAACACAAAGATGGCA+TGG | + | chr4.1:71903631-71903650 | None:intergenic | 40.0% | |
| ! | TCGTCACTCGCTTAAAGTTT+TGG | - | chr4.1:71903943-71903962 | MS.gene76677:CDS | 40.0% |
| !! | CGCTTAAAGTTTTGGTGTGT+CGG | - | chr4.1:71903951-71903970 | MS.gene76677:CDS | 40.0% |
| AACACAAAGATGGCATGGCA+TGG | + | chr4.1:71903626-71903645 | None:intergenic | 45.0% | |
| AAGATCTACACGAAGACTCG+TGG | - | chr4.1:71903779-71903798 | MS.gene76677:CDS | 45.0% | |
| AATCCAGCTGACCTAGTTGA+AGG | - | chr4.1:71904067-71904086 | MS.gene76677:CDS | 45.0% | |
| ACACAAAGATGGCATGGCAT+GGG | + | chr4.1:71903625-71903644 | None:intergenic | 45.0% | |
| AGCTTTGCCCTTCTTCTCTT+TGG | - | chr4.1:71903824-71903843 | MS.gene76677:CDS | 45.0% | |
| CAACTAGGTCAGCTGGATTT+GGG | + | chr4.1:71904066-71904085 | None:intergenic | 45.0% | |
| GAGTGACGAGCAAGTATAAC+TGG | + | chr4.1:71903932-71903951 | None:intergenic | 45.0% | |
| GATGCAGATGGATGTCAAGA+AGG | + | chr4.1:71903738-71903757 | None:intergenic | 45.0% | |
| GTGTCGGATAATAGAAGCGA+TGG | - | chr4.1:71903967-71903986 | MS.gene76677:CDS | 45.0% | |
| TCAACTAGGTCAGCTGGATT+TGG | + | chr4.1:71904067-71904086 | None:intergenic | 45.0% | |
| TGCATCTACTGCTCGCTAAT+CGG | - | chr4.1:71903752-71903771 | MS.gene76677:CDS | 45.0% | |
| TGGGTTGTGCTCTTCGTATT+GGG | - | chr4.1:71903899-71903918 | MS.gene76677:CDS | 45.0% | |
| ! | TTGGGTTGTGCTCTTCGTAT+TGG | - | chr4.1:71903898-71903917 | MS.gene76677:CDS | 45.0% |
| !! | GGTTGTTTTGTTGCTTCCAC+TGG | + | chr4.1:71904041-71904060 | None:intergenic | 45.0% |
| !!! | GCTGCCGCAACAACTTTTTT+GGG | - | chr4.1:71903880-71903899 | MS.gene76677:CDS | 45.0% |
| !!! | TGCTGCCGCAACAACTTTTT+TGG | - | chr4.1:71903879-71903898 | MS.gene76677:CDS | 45.0% |
| AAGTTGTTGCGGCAGCAAGA+TGG | + | chr4.1:71903876-71903895 | None:intergenic | 50.0% | |
| ATGGCATGGCATGGGATTGA+AGG | + | chr4.1:71903617-71903636 | None:intergenic | 50.0% | |
| CTACCTTCAACTAGGTCAGC+TGG | + | chr4.1:71904073-71904092 | None:intergenic | 50.0% | |
| TAGCGAGCAGTAGATGCAGA+TGG | + | chr4.1:71903750-71903769 | None:intergenic | 50.0% | |
| TAGGTCAGCTGGATTTGGGT+AGG | + | chr4.1:71904062-71904081 | None:intergenic | 50.0% | |
| TGGCATGGCATGGGATTGAA+GGG | + | chr4.1:71903616-71903635 | None:intergenic | 50.0% | |
| CGGCAGCAAGATGGAGAGAA+GGG | + | chr4.1:71903867-71903886 | None:intergenic | 55.0% | |
| GCGGCAGCAAGATGGAGAGA+AGG | + | chr4.1:71903868-71903887 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 71903542 | 71904099 | 71903542 | ID=MS.gene76677 |
| chr4.1 | mRNA | 71903542 | 71904099 | 71903542 | ID=MS.gene76677.t1;Parent=MS.gene76677 |
| chr4.1 | exon | 71903542 | 71904099 | 71903542 | ID=MS.gene76677.t1.exon1;Parent=MS.gene76677.t1 |
| chr4.1 | CDS | 71903542 | 71904099 | 71903542 | ID=cds.MS.gene76677.t1;Parent=MS.gene76677.t1 |
| Gene Sequence |
| Protein sequence |
