Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76801.t1 | XP_004490115.1 | 85.5 | 110 | 15 | 1 | 1 | 110 | 1 | 109 | 1.30E-43 | 185.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76801.t1 | A0A1S2XJ41 | 85.5 | 110 | 15 | 1 | 1 | 110 | 1 | 109 | 9.7e-44 | 185.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050170 | MS.gene76801 | 0.823229 | 1.55E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene76801 | 0.814138 | 1.79E-51 | -1.69E-46 |
| MS.gene051963 | MS.gene76801 | -0.800497 | 1.40E-48 | -1.69E-46 |
| MS.gene052494 | MS.gene76801 | -0.817465 | 3.25E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene76801 | 0.826948 | 2.05E-54 | -1.69E-46 |
| MS.gene056266 | MS.gene76801 | 0.844545 | 7.07E-59 | -1.69E-46 |
| MS.gene056508 | MS.gene76801 | 0.805736 | 1.16E-49 | -1.69E-46 |
| MS.gene059545 | MS.gene76801 | -0.805245 | 1.46E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene76801 | 0.814241 | 1.70E-51 | -1.69E-46 |
| MS.gene06042 | MS.gene76801 | -0.816305 | 5.92E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76801.t1 | MTR_5g042520 | 84.211 | 114 | 16 | 1 | 1 | 112 | 1 | 114 | 1.48e-60 | 187 |
| MS.gene76801.t1 | MTR_5g042520 | 85.714 | 112 | 14 | 1 | 1 | 110 | 1 | 112 | 2.11e-60 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76801.t1 | AT1G13990 | 46.617 | 133 | 50 | 5 | 1 | 114 | 1 | 131 | 2.67e-19 | 80.9 |
| MS.gene76801.t1 | AT1G13990 | 47.287 | 129 | 47 | 5 | 1 | 110 | 1 | 127 | 5.76e-19 | 80.5 |
| MS.gene76801.t1 | AT1G13990 | 47.287 | 129 | 47 | 5 | 1 | 110 | 1 | 127 | 7.29e-19 | 80.1 |
Find 48 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACTCATCCGAGCTTCC+AGG | 0.336242 | 5.2:+31790780 | None:intergenic |
| CGACGGAAGGGGTTGGTGGG+AGG | 0.341218 | 5.2:+31790975 | None:intergenic |
| GTGGCGACGGAAGGGGTTGG+TGG | 0.346987 | 5.2:+31790971 | None:intergenic |
| CATATGGATGGTAAAATTCC+TGG | 0.374107 | 5.2:-31790798 | MS.gene76801:CDS |
| AAGAAGCATAAAATTAAAGT+TGG | 0.391094 | 5.2:+31790649 | None:intergenic |
| GTTGGTGGGAGGGAGGACTA+TGG | 0.393546 | 5.2:+31790986 | None:intergenic |
| GGCGGGTGTGGCGACGGAAG+GGG | 0.401705 | 5.2:+31790964 | None:intergenic |
| AGAGAGAGAGGCGGCGGGTG+TGG | 0.404308 | 5.2:+31790952 | None:intergenic |
| GCGGCGGGTGTGGCGACGGA+AGG | 0.409468 | 5.2:+31790962 | None:intergenic |
| CTTCGTTCCTCCTCCTGGTT+CGG | 0.428630 | 5.2:+31790886 | None:intergenic |
| CGATACTTCGTTCCTCCTCC+TGG | 0.435769 | 5.2:+31790881 | None:intergenic |
| AGCAGCGGTGGAGGAGAGGA+CGG | 0.456583 | 5.2:-31790849 | MS.gene76801:CDS |
| GGAAGGGGTTGGTGGGAGGG+AGG | 0.458182 | 5.2:+31790979 | None:intergenic |
| GACGGAAGGGGTTGGTGGGA+GGG | 0.458747 | 5.2:+31790976 | None:intergenic |
| GGTGGAGAAAGCGCTTCATA+TGG | 0.468825 | 5.2:-31790814 | MS.gene76801:CDS |
| TTTGATTCTAGCAGCTATGA+TGG | 0.481586 | 5.2:-31790705 | MS.gene76801:CDS |
| GGAGAAGAGAGAGAGGCGGC+GGG | 0.484370 | 5.2:+31790947 | None:intergenic |
| CGGCGGGTGTGGCGACGGAA+GGG | 0.485102 | 5.2:+31790963 | None:intergenic |
| TGCAAAGTCAGCAGCGGTGG+AGG | 0.488867 | 5.2:-31790858 | MS.gene76801:CDS |
| GGGAGAAGAGAGAGAGGCGG+CGG | 0.493916 | 5.2:+31790946 | None:intergenic |
| AGAGGCGGCGGGTGTGGCGA+CGG | 0.499779 | 5.2:+31790958 | None:intergenic |
| AGAAGAGTCTCGGAGGAAGA+TGG | 0.501382 | 5.2:+31790922 | None:intergenic |
| GCAGCGGTGGAGGAGAGGAC+GGG | 0.502707 | 5.2:-31790848 | MS.gene76801:CDS |
| AGAGGACGGGAGAGATGAAG+AGG | 0.520462 | 5.2:-31790835 | MS.gene76801:CDS |
| CTACACATCGCGTCCGAACC+AGG | 0.520972 | 5.2:-31790899 | MS.gene76801:CDS |
| AGTCAGCAGCGGTGGAGGAG+AGG | 0.526025 | 5.2:-31790853 | MS.gene76801:CDS |
| GGTGTGGCGACGGAAGGGGT+TGG | 0.531215 | 5.2:+31790968 | None:intergenic |
| GGACGGGAGAGATGAAGAGG+TGG | 0.531364 | 5.2:-31790832 | MS.gene76801:CDS |
| TGGCGACGGAAGGGGTTGGT+GGG | 0.533423 | 5.2:+31790972 | None:intergenic |
| GAGTCTCGGAGGAAGATGGC+GGG | 0.543744 | 5.2:+31790926 | None:intergenic |
| ACTATGGAAGGTGATATTGA+AGG | 0.552945 | 5.2:+31791002 | None:intergenic |
| ATCATAGCTGCTAGAATCAA+AGG | 0.555592 | 5.2:+31790707 | None:intergenic |
| TGGTAAAATTCCTGGAAGCT+CGG | 0.563150 | 5.2:-31790790 | MS.gene76801:CDS |
| TCGTGCAAAGTCAGCAGCGG+TGG | 0.577748 | 5.2:-31790861 | MS.gene76801:CDS |
| GATGGCGGGAGAAGAGAGAG+AGG | 0.579533 | 5.2:+31790940 | None:intergenic |
| ATCGCGTCCGAACCAGGAGG+AGG | 0.582438 | 5.2:-31790893 | MS.gene76801:CDS |
| AGAGTCTCGGAGGAAGATGG+CGG | 0.608815 | 5.2:+31790925 | None:intergenic |
| TCCTACGCATATCATAAGCA+CGG | 0.615988 | 5.2:+31790742 | None:intergenic |
| GGCGGGAGAAGAGAGAGAGG+CGG | 0.617937 | 5.2:+31790943 | None:intergenic |
| GAGAAAGCGCTTCATATGGA+TGG | 0.619677 | 5.2:-31790810 | MS.gene76801:CDS |
| CCTACGCATATCATAAGCAC+GGG | 0.621176 | 5.2:+31790743 | None:intergenic |
| CGATGTGTAGAGAAGAGTCT+CGG | 0.639811 | 5.2:+31790912 | None:intergenic |
| CCCGTGCTTATGATATGCGT+AGG | 0.646813 | 5.2:-31790743 | MS.gene76801:CDS |
| ACGCATATCATAAGCACGGG+AGG | 0.665509 | 5.2:+31790746 | None:intergenic |
| GTGGGAGGGAGGACTATGGA+AGG | 0.675207 | 5.2:+31790990 | None:intergenic |
| TGTGTAGAGAAGAGTCTCGG+AGG | 0.689941 | 5.2:+31790915 | None:intergenic |
| GTATCGTGCAAAGTCAGCAG+CGG | 0.717527 | 5.2:-31790864 | MS.gene76801:CDS |
| CACATCGCGTCCGAACCAGG+AGG | 0.764506 | 5.2:-31790896 | MS.gene76801:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTATGGAAGGTGATATTGA+AGG | + | chr5.2:31790677-31790696 | None:intergenic | 35.0% | |
| ATCATAGCTGCTAGAATCAA+AGG | + | chr5.2:31790972-31790991 | None:intergenic | 35.0% | |
| CATATGGATGGTAAAATTCC+TGG | - | chr5.2:31790878-31790897 | MS.gene76801:CDS | 35.0% | |
| TTTGATTCTAGCAGCTATGA+TGG | - | chr5.2:31790971-31790990 | MS.gene76801:CDS | 35.0% | |
| TCCTACGCATATCATAAGCA+CGG | + | chr5.2:31790937-31790956 | None:intergenic | 40.0% | |
| TGGTAAAATTCCTGGAAGCT+CGG | - | chr5.2:31790886-31790905 | MS.gene76801:CDS | 40.0% | |
| CCTACGCATATCATAAGCAC+GGG | + | chr5.2:31790936-31790955 | None:intergenic | 45.0% | |
| CGATGTGTAGAGAAGAGTCT+CGG | + | chr5.2:31790767-31790786 | None:intergenic | 45.0% | |
| !! | GAGAAAGCGCTTCATATGGA+TGG | - | chr5.2:31790866-31790885 | MS.gene76801:CDS | 45.0% |
| AAGAACTCATCCGAGCTTCC+AGG | + | chr5.2:31790899-31790918 | None:intergenic | 50.0% | |
| ACGCATATCATAAGCACGGG+AGG | + | chr5.2:31790933-31790952 | None:intergenic | 50.0% | |
| AGAAGAGTCTCGGAGGAAGA+TGG | + | chr5.2:31790757-31790776 | None:intergenic | 50.0% | |
| CCCGTGCTTATGATATGCGT+AGG | - | chr5.2:31790933-31790952 | MS.gene76801:CDS | 50.0% | |
| GTATCGTGCAAAGTCAGCAG+CGG | - | chr5.2:31790812-31790831 | MS.gene76801:CDS | 50.0% | |
| TGTGTAGAGAAGAGTCTCGG+AGG | + | chr5.2:31790764-31790783 | None:intergenic | 50.0% | |
| !! | GGTGGAGAAAGCGCTTCATA+TGG | - | chr5.2:31790862-31790881 | MS.gene76801:CDS | 50.0% |
| AGAGGACGGGAGAGATGAAG+AGG | - | chr5.2:31790841-31790860 | MS.gene76801:CDS | 55.0% | |
| AGAGTCTCGGAGGAAGATGG+CGG | + | chr5.2:31790754-31790773 | None:intergenic | 55.0% | |
| CGATACTTCGTTCCTCCTCC+TGG | + | chr5.2:31790798-31790817 | None:intergenic | 55.0% | |
| CTTCGTTCCTCCTCCTGGTT+CGG | + | chr5.2:31790793-31790812 | None:intergenic | 55.0% | |
| CTACACATCGCGTCCGAACC+AGG | - | chr5.2:31790777-31790796 | MS.gene76801:CDS | 60.0% | |
| GAGTCTCGGAGGAAGATGGC+GGG | + | chr5.2:31790753-31790772 | None:intergenic | 60.0% | |
| GGACGGGAGAGATGAAGAGG+TGG | - | chr5.2:31790844-31790863 | MS.gene76801:CDS | 60.0% | |
| GTGGGAGGGAGGACTATGGA+AGG | + | chr5.2:31790689-31790708 | None:intergenic | 60.0% | |
| TCGTGCAAAGTCAGCAGCGG+TGG | - | chr5.2:31790815-31790834 | MS.gene76801:CDS | 60.0% | |
| TGCAAAGTCAGCAGCGGTGG+AGG | - | chr5.2:31790818-31790837 | MS.gene76801:CDS | 60.0% | |
| ! | GTTGGTGGGAGGGAGGACTA+TGG | + | chr5.2:31790693-31790712 | None:intergenic | 60.0% |
| !! | GATGGCGGGAGAAGAGAGAG+AGG | + | chr5.2:31790739-31790758 | None:intergenic | 60.0% |
| AGCAGCGGTGGAGGAGAGGA+CGG | - | chr5.2:31790827-31790846 | MS.gene76801:CDS | 65.0% | |
| AGTCAGCAGCGGTGGAGGAG+AGG | - | chr5.2:31790823-31790842 | MS.gene76801:CDS | 65.0% | |
| ATCGCGTCCGAACCAGGAGG+AGG | - | chr5.2:31790783-31790802 | MS.gene76801:CDS | 65.0% | |
| CACATCGCGTCCGAACCAGG+AGG | - | chr5.2:31790780-31790799 | MS.gene76801:CDS | 65.0% | |
| GGAGAAGAGAGAGAGGCGGC+GGG | + | chr5.2:31790732-31790751 | None:intergenic | 65.0% | |
| GGCGGGAGAAGAGAGAGAGG+CGG | + | chr5.2:31790736-31790755 | None:intergenic | 65.0% | |
| GGGAGAAGAGAGAGAGGCGG+CGG | + | chr5.2:31790733-31790752 | None:intergenic | 65.0% | |
| ! | TGGCGACGGAAGGGGTTGGT+GGG | + | chr5.2:31790707-31790726 | None:intergenic | 65.0% |
| !! | GACGGAAGGGGTTGGTGGGA+GGG | + | chr5.2:31790703-31790722 | None:intergenic | 65.0% |
| AGAGAGAGAGGCGGCGGGTG+TGG | + | chr5.2:31790727-31790746 | None:intergenic | 70.0% | |
| GCAGCGGTGGAGGAGAGGAC+GGG | - | chr5.2:31790828-31790847 | MS.gene76801:CDS | 70.0% | |
| GGTGTGGCGACGGAAGGGGT+TGG | + | chr5.2:31790711-31790730 | None:intergenic | 70.0% | |
| GTGGCGACGGAAGGGGTTGG+TGG | + | chr5.2:31790708-31790727 | None:intergenic | 70.0% | |
| !! | CGACGGAAGGGGTTGGTGGG+AGG | + | chr5.2:31790704-31790723 | None:intergenic | 70.0% |
| !! | GGAAGGGGTTGGTGGGAGGG+AGG | + | chr5.2:31790700-31790719 | None:intergenic | 70.0% |
| AGAGGCGGCGGGTGTGGCGA+CGG | + | chr5.2:31790721-31790740 | None:intergenic | 75.0% | |
| CGGCGGGTGTGGCGACGGAA+GGG | + | chr5.2:31790716-31790735 | None:intergenic | 75.0% | |
| GGCGGGTGTGGCGACGGAAG+GGG | + | chr5.2:31790715-31790734 | None:intergenic | 75.0% | |
| ! | GCGGCGGGTGTGGCGACGGA+AGG | + | chr5.2:31790717-31790736 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 31790662 | 31791036 | 31790662 | ID=MS.gene76801 |
| chr5.2 | mRNA | 31790662 | 31791036 | 31790662 | ID=MS.gene76801.t1;Parent=MS.gene76801 |
| chr5.2 | exon | 31790662 | 31791036 | 31790662 | ID=MS.gene76801.t1.exon1;Parent=MS.gene76801.t1 |
| chr5.2 | CDS | 31790662 | 31791036 | 31790662 | ID=cds.MS.gene76801.t1;Parent=MS.gene76801.t1 |
| Gene Sequence |
| Protein sequence |
