Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77074.t1 | RHN44990.1 | 98.8 | 330 | 4 | 0 | 1 | 330 | 1 | 330 | 1.90E-190 | 674.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77074.t1 | P49730 | 85.2 | 330 | 48 | 1 | 1 | 330 | 1 | 329 | 3.9e-163 | 575.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77074.t1 | A0A396GVD1 | 98.8 | 330 | 4 | 0 | 1 | 330 | 1 | 330 | 1.4e-190 | 674.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052367 | MS.gene77074 | 0.820405 | 6.98E-53 | -1.69E-46 |
| MS.gene060565 | MS.gene77074 | 0.800706 | 1.27E-48 | -1.69E-46 |
| MS.gene061260 | MS.gene77074 | 0.805256 | 1.46E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77074 | MS.gene034046 | PPI |
| MS.gene026793 | MS.gene77074 | PPI |
| MS.gene79484 | MS.gene77074 | PPI |
| MS.gene77074 | MS.gene034044 | PPI |
| MS.gene37310 | MS.gene77074 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77074.t1 | MTR_0268s0080 | 98.485 | 330 | 5 | 0 | 1 | 330 | 1 | 330 | 0.0 | 674 |
| MS.gene77074.t1 | MTR_1g050472 | 97.879 | 330 | 7 | 0 | 1 | 330 | 1 | 330 | 0.0 | 669 |
| MS.gene77074.t1 | MTR_7g029110 | 97.576 | 330 | 8 | 0 | 1 | 330 | 1 | 330 | 0.0 | 667 |
| MS.gene77074.t1 | MTR_8g469010 | 92.424 | 330 | 20 | 1 | 1 | 330 | 1 | 325 | 0.0 | 633 |
| MS.gene77074.t1 | MTR_4g117370 | 76.308 | 325 | 76 | 1 | 6 | 330 | 18 | 341 | 0.0 | 528 |
| MS.gene77074.t1 | MTR_6g005270 | 67.442 | 43 | 14 | 0 | 265 | 307 | 25 | 67 | 1.52e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77074.t1 | AT3G27060 | 83.434 | 332 | 53 | 2 | 1 | 330 | 1 | 332 | 0.0 | 555 |
| MS.gene77074.t1 | AT3G23580 | 76.308 | 325 | 76 | 1 | 6 | 330 | 18 | 341 | 0.0 | 515 |
Find 56 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTACATTTCCCAGATTT+TGG | 0.151339 | 7.3:-71125109 | None:intergenic |
| CATTCCATGTATCGCGAAAA+AGG | 0.263160 | 7.3:+71125443 | MS.gene77074:CDS |
| CAATCCAATACCCCAAAATC+TGG | 0.320986 | 7.3:+71125097 | MS.gene77074:CDS |
| TTTGTTGCTGATAGATTATT+GGG | 0.346573 | 7.3:+71125819 | MS.gene77074:CDS |
| GTTTGTTGCTGATAGATTAT+TGG | 0.356307 | 7.3:+71125818 | MS.gene77074:CDS |
| AGGCTGATTGGGCGATGAAA+TGG | 0.358474 | 7.3:+71125463 | MS.gene77074:CDS |
| GGTGCGAGAAGTGGTTCTTC+TGG | 0.368731 | 7.3:-71125048 | None:intergenic |
| GTTGCATTTGCTTGTGTAGA+AGG | 0.373024 | 7.3:+71125519 | MS.gene77074:CDS |
| ATGTTCAGAATCCGTTTGAT+TGG | 0.390491 | 7.3:+71125868 | MS.gene77074:CDS |
| GCGCTTCCTTGCGCGTTGGT+TGG | 0.401864 | 7.3:+71125762 | MS.gene77074:CDS |
| GAAAATCGCGCAGAAGCTTC+CGG | 0.409737 | 7.3:-71125553 | None:intergenic |
| GGGAACATGCAGAATCGGTC+TGG | 0.418807 | 7.3:-71125075 | None:intergenic |
| ATGCAGAATCGGTCTGGGTT+TGG | 0.432958 | 7.3:-71125069 | None:intergenic |
| TCAGAATCCGTTTGATTGGA+TGG | 0.443597 | 7.3:+71125872 | MS.gene77074:CDS |
| CTGTTATGAATAGCTTGAAT+GGG | 0.453833 | 7.3:+71125961 | MS.gene77074:CDS |
| TCTGTTATGAATAGCTTGAA+TGG | 0.458759 | 7.3:+71125960 | MS.gene77074:CDS |
| CGACGCGCTTCCTTGCGCGT+TGG | 0.462960 | 7.3:+71125758 | MS.gene77074:CDS |
| GGCGGTCCAGAATGAGGCTT+CGG | 0.476778 | 7.3:-71125136 | None:intergenic |
| ATGGAATTGATTTCGCTTCA+AGG | 0.479094 | 7.3:+71125891 | MS.gene77074:CDS |
| ATCAATTCCATCCAATCAAA+CGG | 0.480364 | 7.3:-71125879 | None:intergenic |
| GAGCTTATTTCACGCGATGA+AGG | 0.482614 | 7.3:+71125621 | MS.gene77074:CDS |
| GGAACATGCAGAATCGGTCT+GGG | 0.489835 | 7.3:-71125074 | None:intergenic |
| GCAATGCAGTTGAAATTGAA+AGG | 0.492366 | 7.3:+71125724 | MS.gene77074:CDS |
| GGAGGAAAGATCGACTTCTT+CGG | 0.499480 | 7.3:-71125157 | None:intergenic |
| TCTGAGGCACGTGCTTTCTA+CGG | 0.503147 | 7.3:+71125312 | MS.gene77074:CDS |
| AGTGGTTCTTCTGGAAAAGC+AGG | 0.510370 | 7.3:-71125039 | None:intergenic |
| GGGTGAACTTGGTTGTGAAA+AGG | 0.510958 | 7.3:+71125839 | MS.gene77074:CDS |
| GATAGATTATTGGGTGAACT+TGG | 0.513434 | 7.3:+71125828 | MS.gene77074:CDS |
| GTTGTTCCAGTGCTTGAGAT+CGG | 0.515525 | 7.3:-71125178 | None:intergenic |
| GCATTCTTCGCTGCCTCAGA+CGG | 0.515579 | 7.3:+71125240 | MS.gene77074:CDS |
| AATTTCTTTGAGAAGCGCGT+TGG | 0.518105 | 7.3:+71125921 | MS.gene77074:CDS |
| GGATTGGGAACATGCAGAAT+CGG | 0.521690 | 7.3:-71125080 | None:intergenic |
| TTCTTCGGCGGTCCAGAATG+AGG | 0.524296 | 7.3:-71125142 | None:intergenic |
| TTTCCTCCGATCTCAAGCAC+TGG | 0.537267 | 7.3:+71125172 | MS.gene77074:CDS |
| TCTGGGTTTGGTGCGAGAAG+TGG | 0.546005 | 7.3:-71125057 | None:intergenic |
| CAATGCAGTTGAAATTGAAA+GGG | 0.547072 | 7.3:+71125725 | MS.gene77074:CDS |
| TGCGCGTTGGTTGGAATGAA+CGG | 0.550794 | 7.3:+71125771 | MS.gene77074:CDS |
| TGGAATTGATTTCGCTTCAA+GGG | 0.551257 | 7.3:+71125892 | MS.gene77074:CDS |
| AATCCAATACCCCAAAATCT+GGG | 0.556684 | 7.3:+71125098 | MS.gene77074:CDS |
| TGAAATAAGCTCAATTGAGA+AGG | 0.556967 | 7.3:-71125610 | None:intergenic |
| TTCGAGGACAATTCCGTCTG+AGG | 0.567105 | 7.3:-71125253 | None:intergenic |
| ATTGTCCTCGAAAATCTCGC+TGG | 0.568595 | 7.3:+71125264 | MS.gene77074:CDS |
| TCCTCGAAAATCTCGCTGGA+AGG | 0.571616 | 7.3:+71125268 | MS.gene77074:CDS |
| CACTGGAACAACCTCACAGA+CGG | 0.586656 | 7.3:+71125189 | MS.gene77074:CDS |
| TTCATTCCAACCAACGCGCA+AGG | 0.588514 | 7.3:-71125768 | None:intergenic |
| ATGAATAGCTTGAATGGGAA+TGG | 0.603815 | 7.3:+71125966 | MS.gene77074:CDS |
| CGAGGACGTGTGAGATGAAG+TGG | 0.610997 | 7.3:-71125218 | None:intergenic |
| ACTAACTTCAGAGCGTGTGA+AGG | 0.618034 | 7.3:+71125692 | MS.gene77074:CDS |
| GTTCCAGTGCTTGAGATCGG+AGG | 0.623496 | 7.3:-71125175 | None:intergenic |
| CTGAGGCAGCGAAGAATGCG+AGG | 0.634916 | 7.3:-71125236 | None:intergenic |
| TCAATTGAGAAGGTTAAACC+CGG | 0.661224 | 7.3:-71125600 | None:intergenic |
| GAAAGAAGTTCAAGTCTCTG+AGG | 0.686151 | 7.3:+71125296 | MS.gene77074:CDS |
| GGAAAGATCGACTTCTTCGG+CGG | 0.687608 | 7.3:-71125154 | None:intergenic |
| AGTGGCGTTCGCCGTCTGTG+AGG | 0.694180 | 7.3:-71125200 | None:intergenic |
| GAAGAGAATACATCTCAGAG+TGG | 0.703975 | 7.3:-71125359 | None:intergenic |
| GCGTTGGTTGGAATGAACGG+TGG | 0.708152 | 7.3:+71125774 | MS.gene77074:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTTGCTGATAGATTATT+GGG | + | chr7.3:71125819-71125838 | MS.gene77074:CDS | 25.0% |
| ATCAATTCCATCCAATCAAA+CGG | - | chr7.3:71125882-71125901 | None:intergenic | 30.0% | |
| CAATGCAGTTGAAATTGAAA+GGG | + | chr7.3:71125725-71125744 | MS.gene77074:CDS | 30.0% | |
| CTGTTATGAATAGCTTGAAT+GGG | + | chr7.3:71125961-71125980 | MS.gene77074:CDS | 30.0% | |
| TCTGTTATGAATAGCTTGAA+TGG | + | chr7.3:71125960-71125979 | MS.gene77074:CDS | 30.0% | |
| TGAAATAAGCTCAATTGAGA+AGG | - | chr7.3:71125613-71125632 | None:intergenic | 30.0% | |
| ! | GTTTGTTGCTGATAGATTAT+TGG | + | chr7.3:71125818-71125837 | MS.gene77074:CDS | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+GGG | - | chr7.3:71125111-71125130 | None:intergenic | 30.0% |
| ! | TTTGTACATTTCCCAGATTT+TGG | - | chr7.3:71125112-71125131 | None:intergenic | 30.0% |
| !!! | ATTTTCTGGTTGAAAAAACG+CGG | + | chr7.3:71125570-71125589 | MS.gene77074:CDS | 30.0% |
| !!! | TGCTGATTTTTGCTTTAGTT+TGG | + | chr7.3:71125992-71126011 | MS.gene77074:CDS | 30.0% |
| AATCCAATACCCCAAAATCT+GGG | + | chr7.3:71125098-71125117 | MS.gene77074:CDS | 35.0% | |
| ATGAATAGCTTGAATGGGAA+TGG | + | chr7.3:71125966-71125985 | MS.gene77074:CDS | 35.0% | |
| ATGTTCAGAATCCGTTTGAT+TGG | + | chr7.3:71125868-71125887 | MS.gene77074:CDS | 35.0% | |
| GATAGATTATTGGGTGAACT+TGG | + | chr7.3:71125828-71125847 | MS.gene77074:CDS | 35.0% | |
| GCAATGCAGTTGAAATTGAA+AGG | + | chr7.3:71125724-71125743 | MS.gene77074:CDS | 35.0% | |
| TCAATTGAGAAGGTTAAACC+CGG | - | chr7.3:71125603-71125622 | None:intergenic | 35.0% | |
| ! | ATGGAATTGATTTCGCTTCA+AGG | + | chr7.3:71125891-71125910 | MS.gene77074:CDS | 35.0% |
| ! | GGATGTTTTCAATTGCAATC+TGG | - | chr7.3:71125341-71125360 | None:intergenic | 35.0% |
| ! | GTAGAAGGAATTTTCTTCTC+CGG | + | chr7.3:71125534-71125553 | MS.gene77074:CDS | 35.0% |
| ! | TGGAATTGATTTCGCTTCAA+GGG | + | chr7.3:71125892-71125911 | MS.gene77074:CDS | 35.0% |
| ! | TGTACATTTCCCAGATTTTG+GGG | - | chr7.3:71125110-71125129 | None:intergenic | 35.0% |
| !!! | AGATTTTGGGGTATTGGATT+GGG | - | chr7.3:71125098-71125117 | None:intergenic | 35.0% |
| AAAAAACGCGGTTTAATGCC+GGG | + | chr7.3:71125582-71125601 | MS.gene77074:CDS | 40.0% | |
| CAATCCAATACCCCAAAATC+TGG | + | chr7.3:71125097-71125116 | MS.gene77074:CDS | 40.0% | |
| CATTCCATGTATCGCGAAAA+AGG | + | chr7.3:71125443-71125462 | MS.gene77074:CDS | 40.0% | |
| GAAAAAACGCGGTTTAATGC+CGG | + | chr7.3:71125581-71125600 | MS.gene77074:CDS | 40.0% | |
| GAAAGAAGTTCAAGTCTCTG+AGG | + | chr7.3:71125296-71125315 | MS.gene77074:CDS | 40.0% | |
| GAAGAGAATACATCTCAGAG+TGG | - | chr7.3:71125362-71125381 | None:intergenic | 40.0% | |
| GTTGCATTTGCTTGTGTAGA+AGG | + | chr7.3:71125519-71125538 | MS.gene77074:CDS | 40.0% | |
| TATCGCGAAAAAGGCTGATT+GGG | + | chr7.3:71125452-71125471 | MS.gene77074:CDS | 40.0% | |
| TCAGAATCCGTTTGATTGGA+TGG | + | chr7.3:71125872-71125891 | MS.gene77074:CDS | 40.0% | |
| ! | AATTTCTTTGAGAAGCGCGT+TGG | + | chr7.3:71125921-71125940 | MS.gene77074:CDS | 40.0% |
| !! | CTTTTTCGCGATACATGGAA+TGG | - | chr7.3:71125445-71125464 | None:intergenic | 40.0% |
| !!! | CAGATTTTGGGGTATTGGAT+TGG | - | chr7.3:71125099-71125118 | None:intergenic | 40.0% |
| !!! | TTTCCCAGATTTTGGGGTAT+TGG | - | chr7.3:71125104-71125123 | None:intergenic | 40.0% |
| AAAAAGCCGAAGCCTCATTC+TGG | + | chr7.3:71125130-71125149 | MS.gene77074:CDS | 45.0% | |
| ACTAACTTCAGAGCGTGTGA+AGG | + | chr7.3:71125692-71125711 | MS.gene77074:CDS | 45.0% | |
| ATTGTCCTCGAAAATCTCGC+TGG | + | chr7.3:71125264-71125283 | MS.gene77074:CDS | 45.0% | |
| GAGCTTATTTCACGCGATGA+AGG | + | chr7.3:71125621-71125640 | MS.gene77074:CDS | 45.0% | |
| GGATTGGGAACATGCAGAAT+CGG | - | chr7.3:71125083-71125102 | None:intergenic | 45.0% | |
| GGGTGAACTTGGTTGTGAAA+AGG | + | chr7.3:71125839-71125858 | MS.gene77074:CDS | 45.0% | |
| GTATCGCGAAAAAGGCTGAT+TGG | + | chr7.3:71125451-71125470 | MS.gene77074:CDS | 45.0% | |
| GTTGTTCCAGTGCTTGAGAT+CGG | - | chr7.3:71125181-71125200 | None:intergenic | 45.0% | |
| ! | AGTGGTTCTTCTGGAAAAGC+AGG | - | chr7.3:71125042-71125061 | None:intergenic | 45.0% |
| ! | GGAGGAAAGATCGACTTCTT+CGG | - | chr7.3:71125160-71125179 | None:intergenic | 45.0% |
| !! | TCAGCCTTTTTCGCGATACA+TGG | - | chr7.3:71125450-71125469 | None:intergenic | 45.0% |
| AGGCTGATTGGGCGATGAAA+TGG | + | chr7.3:71125463-71125482 | MS.gene77074:CDS | 50.0% | |
| ATGCAGAATCGGTCTGGGTT+TGG | - | chr7.3:71125072-71125091 | None:intergenic | 50.0% | |
| CACTGGAACAACCTCACAGA+CGG | + | chr7.3:71125189-71125208 | MS.gene77074:CDS | 50.0% | |
| GAAAATCGCGCAGAAGCTTC+CGG | - | chr7.3:71125556-71125575 | None:intergenic | 50.0% | |
| GGAACATGCAGAATCGGTCT+GGG | - | chr7.3:71125077-71125096 | None:intergenic | 50.0% | |
| TCCTCGAAAATCTCGCTGGA+AGG | + | chr7.3:71125268-71125287 | MS.gene77074:CDS | 50.0% | |
| TTCATTCCAACCAACGCGCA+AGG | - | chr7.3:71125771-71125790 | None:intergenic | 50.0% | |
| TTCGAGGACAATTCCGTCTG+AGG | - | chr7.3:71125256-71125275 | None:intergenic | 50.0% | |
| TTTCCTCCGATCTCAAGCAC+TGG | + | chr7.3:71125172-71125191 | MS.gene77074:CDS | 50.0% | |
| ! | ACCTTCCAGCGAGATTTTCG+AGG | - | chr7.3:71125272-71125291 | None:intergenic | 50.0% |
| ! | GAAGCTTCTGCGCGATTTTC+TGG | + | chr7.3:71125556-71125575 | MS.gene77074:CDS | 50.0% |
| ! | GGAAAGATCGACTTCTTCGG+CGG | - | chr7.3:71125157-71125176 | None:intergenic | 50.0% |
| ! | TCTGAGGCACGTGCTTTCTA+CGG | + | chr7.3:71125312-71125331 | MS.gene77074:CDS | 50.0% |
| !! | TGCGCGTTGGTTGGAATGAA+CGG | + | chr7.3:71125771-71125790 | MS.gene77074:CDS | 50.0% |
| CGAGGACGTGTGAGATGAAG+TGG | - | chr7.3:71125221-71125240 | None:intergenic | 55.0% | |
| GCATTCTTCGCTGCCTCAGA+CGG | + | chr7.3:71125240-71125259 | MS.gene77074:CDS | 55.0% | |
| GGGAACATGCAGAATCGGTC+TGG | - | chr7.3:71125078-71125097 | None:intergenic | 55.0% | |
| GGTGCGAGAAGTGGTTCTTC+TGG | - | chr7.3:71125051-71125070 | None:intergenic | 55.0% | |
| GTTCCAGTGCTTGAGATCGG+AGG | - | chr7.3:71125178-71125197 | None:intergenic | 55.0% | |
| TTCTTCGGCGGTCCAGAATG+AGG | - | chr7.3:71125145-71125164 | None:intergenic | 55.0% | |
| !! | GCGTTGGTTGGAATGAACGG+TGG | + | chr7.3:71125774-71125793 | MS.gene77074:CDS | 55.0% |
| !! | TCTGGGTTTGGTGCGAGAAG+TGG | - | chr7.3:71125060-71125079 | None:intergenic | 55.0% |
| CTGAGGCAGCGAAGAATGCG+AGG | - | chr7.3:71125239-71125258 | None:intergenic | 60.0% | |
| GGCGGTCCAGAATGAGGCTT+CGG | - | chr7.3:71125139-71125158 | None:intergenic | 60.0% | |
| AGTGGCGTTCGCCGTCTGTG+AGG | - | chr7.3:71125203-71125222 | None:intergenic | 65.0% | |
| ! | GCGCTTCCTTGCGCGTTGGT+TGG | + | chr7.3:71125762-71125781 | MS.gene77074:CDS | 65.0% |
| CGACGCGCTTCCTTGCGCGT+TGG | + | chr7.3:71125758-71125777 | MS.gene77074:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 71125036 | 71126028 | 71125036 | ID=MS.gene77074 |
| chr7.3 | mRNA | 71125036 | 71126028 | 71125036 | ID=MS.gene77074.t1;Parent=MS.gene77074 |
| chr7.3 | exon | 71125036 | 71126028 | 71125036 | ID=MS.gene77074.t1.exon1;Parent=MS.gene77074.t1 |
| chr7.3 | CDS | 71125036 | 71126028 | 71125036 | ID=cds.MS.gene77074.t1;Parent=MS.gene77074.t1 |
| Gene Sequence |
| Protein sequence |
