AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77103

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77103.t1 MTR_2g060565 63.946 147 31 4 99 245 13 137 2.75e-52 169
MS.gene77103.t1 MTR_5g046240 56.436 101 27 3 1 84 730 830 1.89e-24 104
MS.gene77103.t1 MTR_5g046240 71.429 70 12 1 249 310 1405 1474 1.03e-22 99.0
MS.gene77103.t1 MTR_1g031840 71.429 70 12 1 249 310 688 757 1.04e-22 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGGATAATGAAGATATTT+TGG 0.185566 2.4:-32607449 MS.gene77103:intron
GAAGCTTTACAGGATCTTTA+TGG 0.205527 2.4:-32608258 MS.gene77103:intron
CTGATCTTTGGAGAAAATTC+TGG 0.215165 2.4:+32609010 None:intergenic
CACATGCTTCTCAGAGTTCT+TGG 0.231355 2.4:-32609076 MS.gene77103:CDS
ATCAACTCAAGTCTGATCTT+TGG 0.258724 2.4:+32608998 None:intergenic
TGAAGTATTTGTACAAAATC+AGG 0.279723 2.4:-32608294 MS.gene77103:CDS
TTATCTATCGATTTCCTTGT+TGG 0.306983 2.4:-32608320 MS.gene77103:CDS
AGGATCATTTGCATATACTA+TGG 0.316298 2.4:-32607374 MS.gene77103:CDS
TGATGGAAATTCCTCTCTTC+TGG 0.319956 2.4:-32607024 MS.gene77103:CDS
TGATTGACTTTGGCATAAAC+TGG 0.324590 2.4:-32609189 MS.gene77103:CDS
AACGGCAGGACCTGGGGATA+TGG 0.347297 2.4:-32608571 MS.gene77103:intron
TCTTGGAAGGGTGACATTGC+AGG 0.350974 2.4:-32609059 MS.gene77103:CDS
GACTTGAGTTGATCAAATAT+TGG 0.363434 2.4:-32608988 MS.gene77103:CDS
ATAAGTTATTGTTGCGCAAC+AGG 0.376055 2.4:-32607306 MS.gene77103:CDS
CTTGACAGGGCCGCGATTTG+AGG 0.381522 2.4:-32607394 MS.gene77103:CDS
TGTTGCGCAACAGGCTTGTT+GGG 0.389869 2.4:-32607297 MS.gene77103:CDS
GGAGGTTTATGTCAAGTCAT+AGG 0.391450 2.4:-32607428 MS.gene77103:CDS
CCAACTCCCACACCTGGAAT+TGG 0.406541 2.4:+32608750 None:intergenic
AGGTTTCTGATGCCCTTGAC+AGG 0.426561 2.4:-32607408 MS.gene77103:CDS
TGCAGAGGAGGACCAATTCC+AGG 0.427136 2.4:-32608762 MS.gene77103:intron
TTGTTGCGCAACAGGCTTGT+TGG 0.433583 2.4:-32607298 MS.gene77103:CDS
GTGACATTGCAGGAAGCTAA+TGG 0.452398 2.4:-32609049 MS.gene77103:CDS
CCTTGCAATTTAGATGATTG+TGG 0.474589 2.4:+32609148 None:intergenic
TTCTGCGACTTCTTTGTCGT+GGG 0.475663 2.4:-32609116 MS.gene77103:CDS
GTTGTTATAGAGAGACCATT+GGG 0.482895 2.4:-32608549 MS.gene77103:CDS
CTCAAATCGCGGCCCTGTCA+AGG 0.484116 2.4:+32607395 None:intergenic
TCTGCATAACATCAATTCAA+TGG 0.493057 2.4:+32608779 None:intergenic
AAAAGAGTTGGTTGGAGTTG+AGG 0.497118 2.4:-32607070 MS.gene77103:CDS
TTTGGTTCGATGATTGACTT+TGG 0.500665 2.4:-32609199 None:intergenic
CAGGTATGAAATATGAATGA+TGG 0.502337 2.4:+32607337 None:intergenic
GAGGATGAAAGAATTGATTG+AGG 0.520698 2.4:-32607051 MS.gene77103:CDS
CTCTCTTTGAGAGATTATGC+TGG 0.521446 2.4:-32607273 MS.gene77103:CDS
TTTCTGCGACTTCTTTGTCG+TGG 0.523486 2.4:-32609117 MS.gene77103:CDS
TGCTTCTCAGAGTTCTTGGA+AGG 0.525323 2.4:-32609072 MS.gene77103:CDS
GAAGTATTTGTACAAAATCA+GGG 0.529854 2.4:-32608293 MS.gene77103:CDS
AAATTCCTCTCTTCTGGCAC+AGG 0.533778 2.4:-32607018 MS.gene77103:CDS
CCACAATCATCTAAATTGCA+AGG 0.538743 2.4:-32609148 MS.gene77103:CDS
GGTTGTTATAGAGAGACCAT+TGG 0.546544 2.4:-32608550 MS.gene77103:CDS
GGCACAGGTGAACAAGTTGA+AGG 0.548409 2.4:-32607003 MS.gene77103:CDS
TATGTTTAACGGCAGGACCT+GGG 0.549650 2.4:-32608578 MS.gene77103:intron
CGCAACAATAACTTATCCAC+AGG 0.553250 2.4:+32607313 None:intergenic
CACAATCATCTAAATTGCAA+GGG 0.561016 2.4:-32609147 MS.gene77103:CDS
TCTCTTTGAGAGATTATGCT+GGG 0.563044 2.4:-32607272 MS.gene77103:CDS
CAAGCAATCAACATCTGACA+TGG 0.564410 2.4:-32607096 MS.gene77103:CDS
TCAAATCGCGGCCCTGTCAA+GGG 0.565264 2.4:+32607396 None:intergenic
CATGGATGAAAAGAGTTGGT+TGG 0.567021 2.4:-32607078 MS.gene77103:CDS
ATCATCTAAATTGCAAGGGG+GGG 0.574244 2.4:-32609143 MS.gene77103:CDS
GGTTTCTGATGCCCTTGACA+GGG 0.580132 2.4:-32607407 MS.gene77103:CDS
CAATAACTTATCCACAGGAC+AGG 0.581405 2.4:+32607318 None:intergenic
GGAGAAAATTCTGGAAGACA+TGG 0.586981 2.4:+32609019 None:intergenic
TCTATAACAACCATATCCCC+AGG 0.591618 2.4:+32608561 None:intergenic
GTTGATCAAATATTGGCACC+TGG 0.594014 2.4:-32608981 MS.gene77103:CDS
GAATTGATTGAGGCATGTGA+TGG 0.598135 2.4:-32607041 MS.gene77103:CDS
CAGATGTTGATTGCTTGCTA+TGG 0.601126 2.4:+32607102 None:intergenic
GAATTGATGTTATGCAGAGG+AGG 0.602718 2.4:-32608774 MS.gene77103:CDS
ATTCAGCATTATCATTGACA+TGG 0.604110 2.4:-32608355 MS.gene77103:intron
AATCATCTAAATTGCAAGGG+GGG 0.609804 2.4:-32609144 MS.gene77103:CDS
ATATGAATGATGGAAGGTGA+TGG 0.610473 2.4:+32607347 None:intergenic
CATATTTCATACCTGTCCTG+TGG 0.618580 2.4:-32607329 MS.gene77103:CDS
ATGTTTAACGGCAGGACCTG+GGG 0.629545 2.4:-32608577 MS.gene77103:intron
GTTCACCTGTGCCAGAAGAG+AGG 0.629884 2.4:+32607013 None:intergenic
AGGATACCTTGAACCTGCAG+AGG 0.638345 2.4:-32607971 MS.gene77103:intron
GCTTCTCAGAGTTCTTGGAA+GGG 0.641030 2.4:-32609071 MS.gene77103:CDS
CAAATACTTCAATTCCAACA+AGG 0.642031 2.4:+32608306 None:intergenic
TGCAAATGATCCTCAAATCG+CGG 0.643658 2.4:+32607384 None:intergenic
CTGACATGGATGAAAAGAGT+TGG 0.644103 2.4:-32607082 MS.gene77103:CDS
TGCTGGGAAACAGCAACATG+AGG 0.645299 2.4:-32607256 MS.gene77103:intron
ACATGGAACAATAAACCCAA+TGG 0.645346 2.4:+32608534 None:intergenic
ATTGAATTGATGTTATGCAG+AGG 0.645749 2.4:-32608777 MS.gene77103:CDS
TATTTGTACAAAATCAGGGT+GGG 0.645769 2.4:-32608289 MS.gene77103:CDS
ACAATCATCTAAATTGCAAG+GGG 0.648757 2.4:-32609146 MS.gene77103:CDS
AGTACTTCAAAACAGAGACA+TGG 0.650829 2.4:+32608517 None:intergenic
TATGAAATATGAATGATGGA+AGG 0.668826 2.4:+32607341 None:intergenic
CAATCATCTAAATTGCAAGG+GGG 0.693581 2.4:-32609145 MS.gene77103:CDS
TGAGAAAATTCTGTGTACAG+AGG 0.700326 2.4:+32608490 None:intergenic
GTATTTGTACAAAATCAGGG+TGG 0.744705 2.4:-32608290 MS.gene77103:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGGTAAAAGTTAT+TGG - chr2.4:32608452-32608471 MS.gene77103:intron 15.0%
!! ATATACTTAATTGAGAATAT+GGG - chr2.4:32608354-32608373 MS.gene77103:intron 15.0%
!! TATATACTTAATTGAGAATA+TGG - chr2.4:32608353-32608372 MS.gene77103:intron 15.0%
!!! CCAAAATAAACAAATTATTT+TGG - chr2.4:32607971-32607990 MS.gene77103:intron 15.0%
!!! CCAAAATAATTTGTTTATTT+TGG + chr2.4:32607974-32607993 None:intergenic 15.0%
!!! CTTATTTTATAGATAATGTT+AGG - chr2.4:32608643-32608662 MS.gene77103:intron 15.0%
!!! TTCAAAAATTTAAAGTAAGT+AGG + chr2.4:32607526-32607545 None:intergenic 15.0%
!! TCAACATAATCAATATTGTT+TGG + chr2.4:32608702-32608721 None:intergenic 20.0%
!!! AAATTCTTTAAAGCGTTAAA+AGG - chr2.4:32608477-32608496 MS.gene77103:intron 20.0%
!!! ATTTTATAGATAATGTTAGG+TGG - chr2.4:32608646-32608665 MS.gene77103:intron 20.0%
!!! CTTTTATAAAAGACATTTCA+GGG + chr2.4:32607291-32607310 None:intergenic 20.0%
!!! TCTTTTATAAAAGACATTTC+AGG + chr2.4:32607292-32607311 None:intergenic 20.0%
!!! TGTATCTTATTTGTTCTTAT+TGG - chr2.4:32609002-32609021 MS.gene77103:CDS 20.0%
!!! TTATAAAAGAAAAGTGTTTG+TGG - chr2.4:32607302-32607321 MS.gene77103:CDS 20.0%
!!! TTTTATAGATAATGTTAGGT+GGG - chr2.4:32608647-32608666 MS.gene77103:intron 20.0%
! ATCTAACTAAGAGATACTAA+AGG + chr2.4:32608527-32608546 None:intergenic 25.0%
! ATGAATGACTAATTCATTTG+AGG - chr2.4:32607244-32607263 MS.gene77103:intron 25.0%
! CTTGTATCAAAGCAAAAAAT+TGG - chr2.4:32608439-32608458 MS.gene77103:intron 25.0%
! GCATAAGAAATATTCTATGT+AGG + chr2.4:32608276-32608295 None:intergenic 25.0%
! TAAATATAATGAAACTTGCC+AGG + chr2.4:32608083-32608102 None:intergenic 25.0%
! TTTATAGATAATGTTAGGTG+GGG - chr2.4:32608648-32608667 MS.gene77103:intron 25.0%
!! GAAGTATTTGTACAAAATCA+GGG - chr2.4:32607886-32607905 MS.gene77103:intron 25.0%
!! TATGAAATATGAATGATGGA+AGG + chr2.4:32608841-32608860 None:intergenic 25.0%
!! TGAAGTATTTGTACAAAATC+AGG - chr2.4:32607885-32607904 MS.gene77103:intron 25.0%
ACAATCATCTAAATTGCAAG+GGG - chr2.4:32607033-32607052 MS.gene77103:CDS 30.0%
AGGATCATTTGCATATACTA+TGG - chr2.4:32608805-32608824 MS.gene77103:intron 30.0%
ATTCAGCATTATCATTGACA+TGG - chr2.4:32607824-32607843 MS.gene77103:intron 30.0%
ATTGAATTGATGTTATGCAG+AGG - chr2.4:32607402-32607421 MS.gene77103:CDS 30.0%
CAAATACTTCAATTCCAACA+AGG + chr2.4:32607876-32607895 None:intergenic 30.0%
CACAATCATCTAAATTGCAA+GGG - chr2.4:32607032-32607051 MS.gene77103:CDS 30.0%
CAGGTATGAAATATGAATGA+TGG + chr2.4:32608845-32608864 None:intergenic 30.0%
CTTAGTTAGATTTAGCTGTT+GGG - chr2.4:32608536-32608555 MS.gene77103:CDS 30.0%
TATTTGTACAAAATCAGGGT+GGG - chr2.4:32607890-32607909 MS.gene77103:intron 30.0%
TCTGCATAACATCAATTCAA+TGG + chr2.4:32607403-32607422 None:intergenic 30.0%
TCTTAGTTAGATTTAGCTGT+TGG - chr2.4:32608535-32608554 MS.gene77103:CDS 30.0%
! GAAACAATGTCCAATTTTCA+AGG - chr2.4:32607779-32607798 MS.gene77103:intron 30.0%
! GCAGGATAATGAAGATATTT+TGG - chr2.4:32608730-32608749 MS.gene77103:intron 30.0%
! TAATTATGTTTTACCTCTGC+AGG + chr2.4:32608224-32608243 None:intergenic 30.0%
! TGATAATGCTGAATAGGAAA+AGG + chr2.4:32607818-32607837 None:intergenic 30.0%
! TGTCAATGATAATGCTGAAT+AGG + chr2.4:32607824-32607843 None:intergenic 30.0%
!! AAAAGAAAAGTGTTTGTGGA+AGG - chr2.4:32607306-32607325 MS.gene77103:CDS 30.0%
!! ATGAACTTGAAGAGTATTCA+AGG - chr2.4:32608572-32608591 MS.gene77103:intron 30.0%
!! GACTTGAGTTGATCAAATAT+TGG - chr2.4:32607191-32607210 MS.gene77103:intron 30.0%
!! GGATAATGAAGATATTTTGG+AGG - chr2.4:32608733-32608752 MS.gene77103:intron 30.0%
!! TTATCTATCGATTTCCTTGT+TGG - chr2.4:32607859-32607878 MS.gene77103:intron 30.0%
!!! TGTGGACTTTTCTTTGTTTT+AGG - chr2.4:32607752-32607771 MS.gene77103:intron 30.0%
AATCATCTAAATTGCAAGGG+GGG - chr2.4:32607035-32607054 MS.gene77103:CDS 35.0%
ACATGGAACAATAAACCCAA+TGG + chr2.4:32607648-32607667 None:intergenic 35.0%
AGTACTTCAAAACAGAGACA+TGG + chr2.4:32607665-32607684 None:intergenic 35.0%
ATAAGTTATTGTTGCGCAAC+AGG - chr2.4:32608873-32608892 MS.gene77103:intron 35.0%
ATATCTTCATTATCCTGCAG+TGG + chr2.4:32608728-32608747 None:intergenic 35.0%
ATCAACTCAAGTCTGATCTT+TGG + chr2.4:32607184-32607203 None:intergenic 35.0%
ATCTTCCATCTTAATCCATC+AGG + chr2.4:32608972-32608991 None:intergenic 35.0%
CAATCATCTAAATTGCAAGG+GGG - chr2.4:32607034-32607053 MS.gene77103:CDS 35.0%
CCACAATCATCTAAATTGCA+AGG - chr2.4:32607031-32607050 MS.gene77103:CDS 35.0%
CCTTGCAATTTAGATGATTG+TGG + chr2.4:32607034-32607053 None:intergenic 35.0%
GAAGCTTTACAGGATCTTTA+TGG - chr2.4:32607921-32607940 MS.gene77103:intron 35.0%
GAGGATGAAAGAATTGATTG+AGG - chr2.4:32609128-32609147 MS.gene77103:CDS 35.0%
GTAGGTGTTTGGTTGAAATA+AGG - chr2.4:32607330-32607349 MS.gene77103:CDS 35.0%
GTATTTGTACAAAATCAGGG+TGG - chr2.4:32607889-32607908 MS.gene77103:intron 35.0%
GTTGTCAGTTCCTTGAAAAT+TGG + chr2.4:32607792-32607811 None:intergenic 35.0%
GTTGTTATAGAGAGACCATT+GGG - chr2.4:32607630-32607649 MS.gene77103:intron 35.0%
TCAAATGGAATTCAACACAG+TGG + chr2.4:32608108-32608127 None:intergenic 35.0%
TCTGAAGGTAACAAAAGTTC+AGG + chr2.4:32608167-32608186 None:intergenic 35.0%
TCTTCCATCTTAATCCATCA+GGG + chr2.4:32608971-32608990 None:intergenic 35.0%
TGAGAAAATTCTGTGTACAG+AGG + chr2.4:32607692-32607711 None:intergenic 35.0%
TGCCTTGGAACTATGTTTAA+CGG - chr2.4:32607590-32607609 MS.gene77103:intron 35.0%
TGTTTGGTACAACTACGTTA+GGG + chr2.4:32608686-32608705 None:intergenic 35.0%
TTGTTTGGTACAACTACGTT+AGG + chr2.4:32608687-32608706 None:intergenic 35.0%
! ACTAATTCATTTGAGGCAGA+AGG - chr2.4:32607251-32607270 MS.gene77103:intron 35.0%
! ATATGAATGATGGAAGGTGA+TGG + chr2.4:32608835-32608854 None:intergenic 35.0%
! CTAATTCATTTGAGGCAGAA+GGG - chr2.4:32607252-32607271 MS.gene77103:intron 35.0%
! CTGATCTTTGGAGAAAATTC+TGG + chr2.4:32607172-32607191 None:intergenic 35.0%
! TCTCTTTGAGAGATTATGCT+GGG - chr2.4:32608907-32608926 MS.gene77103:intron 35.0%
! TGATTGACTTTGGCATAAAC+TGG - chr2.4:32606990-32607009 MS.gene77103:CDS 35.0%
! TTTTGAATGCTTTTCCCTGA+TGG - chr2.4:32608954-32608973 MS.gene77103:intron 35.0%
!! AAAAAGCATCTACTGTACTC+TGG + chr2.4:32608417-32608436 None:intergenic 35.0%
!! ACTTGAAGAGTATTCAAGGA+TGG - chr2.4:32608576-32608595 MS.gene77103:intron 35.0%
!! CTTGAAGAGTATTCAAGGAT+GGG - chr2.4:32608577-32608596 MS.gene77103:intron 35.0%
!!! GGATACTTTTGAAGCTTTAC+AGG - chr2.4:32607911-32607930 MS.gene77103:intron 35.0%
!!! TTTTCCCTGATGGATTAAGA+TGG - chr2.4:32608964-32608983 MS.gene77103:CDS 35.0%
ACATTCCCTGTAACGCAAAT+TGG + chr2.4:32609041-32609060 None:intergenic 40.0%
ACTGATCCAATTTGCGTTAC+AGG - chr2.4:32609032-32609051 MS.gene77103:CDS 40.0%
AGACGACCTATACCATTCAA+CGG - chr2.4:32607559-32607578 MS.gene77103:intron 40.0%
ATCATCTAAATTGCAAGGGG+GGG - chr2.4:32607036-32607055 MS.gene77103:CDS 40.0%
CAAGCAATCAACATCTGACA+TGG - chr2.4:32609083-32609102 MS.gene77103:CDS 40.0%
CAATAACTTATCCACAGGAC+AGG + chr2.4:32608864-32608883 None:intergenic 40.0%
CATATTTCATACCTGTCCTG+TGG - chr2.4:32608850-32608869 MS.gene77103:intron 40.0%
CGCAACAATAACTTATCCAC+AGG + chr2.4:32608869-32608888 None:intergenic 40.0%
CTGACATGGATGAAAAGAGT+TGG - chr2.4:32609097-32609116 MS.gene77103:CDS 40.0%
CTGATCCAATTTGCGTTACA+GGG - chr2.4:32609033-32609052 MS.gene77103:CDS 40.0%
GAATTGATGTTATGCAGAGG+AGG - chr2.4:32607405-32607424 MS.gene77103:CDS 40.0%
GAATTGATTGAGGCATGTGA+TGG - chr2.4:32609138-32609157 MS.gene77103:CDS 40.0%
GGAGGTTTATGTCAAGTCAT+AGG - chr2.4:32608751-32608770 MS.gene77103:intron 40.0%
GGTTGTTATAGAGAGACCAT+TGG - chr2.4:32607629-32607648 MS.gene77103:intron 40.0%
GTTGATCAAATATTGGCACC+TGG - chr2.4:32607198-32607217 MS.gene77103:intron 40.0%
GTTGTTCCGTTGAATGGTAT+AGG + chr2.4:32607568-32607587 None:intergenic 40.0%
TCTATAACAACCATATCCCC+AGG + chr2.4:32607621-32607640 None:intergenic 40.0%
TGATGGAAATTCCTCTCTTC+TGG - chr2.4:32609155-32609174 MS.gene77103:CDS 40.0%
TGCAAATGATCCTCAAATCG+CGG + chr2.4:32608798-32608817 None:intergenic 40.0%
TGCCGTTAAACATAGTTCCA+AGG + chr2.4:32607595-32607614 None:intergenic 40.0%
TGTCTTCAACTTCTTCAGTG+TGG - chr2.4:32607364-32607383 MS.gene77103:CDS 40.0%
TTATGGTGAGCTTCTCGATA+GGG - chr2.4:32607938-32607957 MS.gene77103:intron 40.0%
TTGGAACTATGTTTAACGGC+AGG - chr2.4:32607594-32607613 MS.gene77103:intron 40.0%
TTTATGGTGAGCTTCTCGAT+AGG - chr2.4:32607937-32607956 MS.gene77103:intron 40.0%
! AAAAGAGTTGGTTGGAGTTG+AGG - chr2.4:32609109-32609128 MS.gene77103:CDS 40.0%
! AATTTTCTCAGTAGCTGGAG+GGG - chr2.4:32607702-32607721 MS.gene77103:intron 40.0%
! AGAATTTTCTCAGTAGCTGG+AGG - chr2.4:32607700-32607719 MS.gene77103:intron 40.0%
! CACAGAATTTTCTCAGTAGC+TGG - chr2.4:32607697-32607716 MS.gene77103:intron 40.0%
! CAGATGTTGATTGCTTGCTA+TGG + chr2.4:32609080-32609099 None:intergenic 40.0%
! CATGGATGAAAAGAGTTGGT+TGG - chr2.4:32609101-32609120 MS.gene77103:CDS 40.0%
! CATTTTGTCCCTTAAAAGCC+AGG + chr2.4:32607219-32607238 None:intergenic 40.0%
! CTCTCTTTGAGAGATTATGC+TGG - chr2.4:32608906-32608925 MS.gene77103:intron 40.0%
! GAATTTTCTCAGTAGCTGGA+GGG - chr2.4:32607701-32607720 MS.gene77103:intron 40.0%
! GGAGAAAATTCTGGAAGACA+TGG + chr2.4:32607163-32607182 None:intergenic 40.0%
!! AAATGCTTTTCGAGAGCTGA+AGG - chr2.4:32608188-32608207 MS.gene77103:intron 40.0%
!! ATATTGGCACCTGGCTTTTA+AGG - chr2.4:32607207-32607226 MS.gene77103:intron 40.0%
!! ATTATGTTGACTTCCACTGC+AGG - chr2.4:32608712-32608731 MS.gene77103:intron 40.0%
!! CTCGAAAAGCATTTCTCTGA+AGG + chr2.4:32608182-32608201 None:intergenic 40.0%
!! GGCTTTTAAGGGACAAAATG+TGG - chr2.4:32607219-32607238 MS.gene77103:intron 40.0%
!! TATTGGCACCTGGCTTTTAA+GGG - chr2.4:32607208-32607227 MS.gene77103:intron 40.0%
AAATTCCTCTCTTCTGGCAC+AGG - chr2.4:32609161-32609180 MS.gene77103:CDS 45.0%
ATTCCAGGTGGCAAGTCAAA+TGG + chr2.4:32608123-32608142 None:intergenic 45.0%
ATTCGACAGTGTATTCCAGG+TGG + chr2.4:32608135-32608154 None:intergenic 45.0%
CACATGCTTCTCAGAGTTCT+TGG - chr2.4:32607103-32607122 MS.gene77103:CDS 45.0%
GCTTCTCAGAGTTCTTGGAA+GGG - chr2.4:32607108-32607127 MS.gene77103:CDS 45.0%
GTGACATTGCAGGAAGCTAA+TGG - chr2.4:32607130-32607149 MS.gene77103:intron 45.0%
TATGTTTAACGGCAGGACCT+GGG - chr2.4:32607601-32607620 MS.gene77103:intron 45.0%
TGCATTCGACAGTGTATTCC+AGG + chr2.4:32608138-32608157 None:intergenic 45.0%
TGCTTCTCAGAGTTCTTGGA+AGG - chr2.4:32607107-32607126 MS.gene77103:CDS 45.0%
TTCTGCGACTTCTTTGTCGT+GGG - chr2.4:32607063-32607082 MS.gene77103:CDS 45.0%
TTGACCAAAATCCTTCGCCA+GGG + chr2.4:32607464-32607483 None:intergenic 45.0%
TTTCTGCGACTTCTTTGTCG+TGG - chr2.4:32607062-32607081 MS.gene77103:CDS 45.0%
TTTGACCAAAATCCTTCGCC+AGG + chr2.4:32607465-32607484 None:intergenic 45.0%
! AAAGTGTTTGTGGAAGGCGT+AGG - chr2.4:32607312-32607331 MS.gene77103:CDS 45.0%
! ATTCATTTGAGGCAGAAGGG+TGG - chr2.4:32607255-32607274 MS.gene77103:intron 45.0%
! CGATGTCAACTTCTTTTGCC+TGG - chr2.4:32608062-32608081 MS.gene77103:intron 45.0%
! GTTTTACCTCTGCAGGTTCA+AGG + chr2.4:32608217-32608236 None:intergenic 45.0%
!! ATTCCATTTGACTTGCCACC+TGG - chr2.4:32608117-32608136 MS.gene77103:intron 45.0%
AGGATACCTTGAACCTGCAG+AGG - chr2.4:32608208-32608227 MS.gene77103:intron 50.0%
AGGTTTCTGATGCCCTTGAC+AGG - chr2.4:32608771-32608790 MS.gene77103:CDS 50.0%
ATGTTTAACGGCAGGACCTG+GGG - chr2.4:32607602-32607621 MS.gene77103:intron 50.0%
CACACATTGCTGCCACCTAA+AGG + chr2.4:32607736-32607755 None:intergenic 50.0%
CTATGTTTAACGGCAGGACC+TGG - chr2.4:32607600-32607619 MS.gene77103:intron 50.0%
CTTTAGGTGGCAGCAATGTG+TGG - chr2.4:32607734-32607753 MS.gene77103:intron 50.0%
GGAGGGGTAAAGAAGCCTTT+AGG - chr2.4:32607718-32607737 MS.gene77103:intron 50.0%
GGGGTAAAGAAGCCTTTAGG+TGG - chr2.4:32607721-32607740 MS.gene77103:intron 50.0%
GGTTTCTGATGCCCTTGACA+GGG - chr2.4:32608772-32608791 MS.gene77103:CDS 50.0%
TCAACGGAACAACACTGCCT+TGG - chr2.4:32607575-32607594 MS.gene77103:intron 50.0%
TCTTGGAAGGGTGACATTGC+AGG - chr2.4:32607120-32607139 MS.gene77103:CDS 50.0%
TGACCAAAATCCTTCGCCAG+GGG + chr2.4:32607463-32607482 None:intergenic 50.0%
TGCTGGGAAACAGCAACATG+AGG - chr2.4:32608923-32608942 MS.gene77103:intron 50.0%
TTGTTGCGCAACAGGCTTGT+TGG - chr2.4:32608881-32608900 MS.gene77103:intron 50.0%
! GGCACAGGTGAACAAGTTGA+AGG - chr2.4:32609176-32609195 MS.gene77103:CDS 50.0%
! GGCAGTGTTGTTCCGTTGAA+TGG + chr2.4:32607574-32607593 None:intergenic 50.0%
! TGTTGCGCAACAGGCTTGTT+GGG - chr2.4:32608882-32608901 MS.gene77103:intron 50.0%
! TTGTGGAAGGCGTAGGTGTT+TGG - chr2.4:32607319-32607338 MS.gene77103:CDS 50.0%
AGGAGGACCAATTCCAGGTG+TGG - chr2.4:32607422-32607441 MS.gene77103:CDS 55.0%
CCAACTCCCACACCTGGAAT+TGG + chr2.4:32607432-32607451 None:intergenic 55.0%
CCAATTCCAGGTGTGGGAGT+TGG - chr2.4:32607429-32607448 MS.gene77103:CDS 55.0%
GGAGGACCAATTCCAGGTGT+GGG - chr2.4:32607423-32607442 MS.gene77103:CDS 55.0%
GTTCACCTGTGCCAGAAGAG+AGG + chr2.4:32609169-32609188 None:intergenic 55.0%
TCAAATCGCGGCCCTGTCAA+GGG + chr2.4:32608786-32608805 None:intergenic 55.0%
TGCAGAGGAGGACCAATTCC+AGG - chr2.4:32607417-32607436 MS.gene77103:CDS 55.0%
AACGGCAGGACCTGGGGATA+TGG - chr2.4:32607608-32607627 MS.gene77103:intron 60.0%
CCAGGTGTGGGAGTTGGATG+AGG - chr2.4:32607435-32607454 MS.gene77103:CDS 60.0%
CCTCATCCAACTCCCACACC+TGG + chr2.4:32607438-32607457 None:intergenic 60.0%
CTCAAATCGCGGCCCTGTCA+AGG + chr2.4:32608787-32608806 None:intergenic 60.0%
CTTGACAGGGCCGCGATTTG+AGG - chr2.4:32608785-32608804 MS.gene77103:CDS 60.0%
TGAGGCAGAAGGGTGGTGAG+TGG - chr2.4:32607262-32607281 MS.gene77103:CDS 60.0%
! GTGCCCCTGGCGAAGGATTT+TGG - chr2.4:32607457-32607476 MS.gene77103:intron 60.0%
! GGAGTTGGATGAGGTGCCCC+TGG - chr2.4:32607444-32607463 MS.gene77103:CDS 65.0%
! GGATGAGGTGCCCCTGGCGA+AGG - chr2.4:32607450-32607469 MS.gene77103:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32606989 32609212 32606989 ID=MS.gene77103
chr2.4 mRNA 32606989 32609212 32606989 ID=MS.gene77103.t1;Parent=MS.gene77103
chr2.4 exon 32608961 32609212 32608961 ID=MS.gene77103.t1.exon1;Parent=MS.gene77103.t1
chr2.4 CDS 32608961 32609212 32608961 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32608763 32608811 32608763 ID=MS.gene77103.t1.exon2;Parent=MS.gene77103.t1
chr2.4 CDS 32608763 32608811 32608763 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32608478 32608585 32608478 ID=MS.gene77103.t1.exon3;Parent=MS.gene77103.t1
chr2.4 CDS 32608478 32608585 32608478 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32608259 32608371 32608259 ID=MS.gene77103.t1.exon4;Parent=MS.gene77103.t1
chr2.4 CDS 32608259 32608371 32608259 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32607972 32608015 32607972 ID=MS.gene77103.t1.exon5;Parent=MS.gene77103.t1
chr2.4 CDS 32607972 32608015 32607972 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32607257 32607467 32607257 ID=MS.gene77103.t1.exon6;Parent=MS.gene77103.t1
chr2.4 CDS 32607257 32607467 32607257 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
chr2.4 exon 32606989 32607147 32606989 ID=MS.gene77103.t1.exon7;Parent=MS.gene77103.t1
chr2.4 CDS 32606989 32607147 32606989 ID=cds.MS.gene77103.t1;Parent=MS.gene77103.t1
Gene Sequence

>MS.gene77103

ATGATTGACTTTGGCATAAACTGGATTGCAACAAGAAGTGATCCACAATCATCTAAATTGCAAGGGGGGGATATTTCTGCGACTTCTTTGTCGTGGGTGTATGCTGCTATGACTCACATGCTTCTCAGAGTTCTTGGAAGGGTGACATTGCAGGAAGCTAATGGACATGTGCCATGTCTTCCAGAATTTTCTCCAAAGATCAGACTTGAGTTGATCAAATATTGGCACCTGGCTTTTAAGGGACAAAATGTGGTGATGAATGACTAATTCATTTGAGGCAGAAGGGTGGTGAGTGGTGACCCTGAAATGTCTTTTATAAAAGAAAAGTGTTTGTGGAAGGCGTAGGTGTTTGGTTGAAATAAGGTCTAAGCTTCATGTCTTCAACTTCTTCAGTGTGGCAGCACATGCAGTCCATTGAATTGATGTTATGCAGAGGAGGACCAATTCCAGGTGTGGGAGTTGGATGAGGTGCCCCTGGCGAAGGATTTTGGTCAAAAACAATTTTATCGTTTCAAACAGATGCAAGATTTCTCACCTACTTACTTTAAATTTTTGAAAATGCAACTGAAGAGACGACCTATACCATTCAACGGAACAACACTGCCTTGGAACTATGTTTAACGGCAGGACCTGGGGATATGGTTGTTATAGAGAGACCATTGGGTTTATTGTTCCATGTCTCTGTTTTGAAGTACTTTGACCTCTGTACACAGAATTTTCTCAGTAGCTGGAGGGGTAAAGAAGCCTTTAGGTGGCAGCAATGTGTGGACTTTTCTTTGTTTTAGGCATTGAAACAATGTCCAATTTTCAAGGAACTGACAACATTCCTTTTCCTATTCAGCATTATCATTGACATGGAAGTTACATTCATTATCTATCGATTTCCTTGTTGGAATTGAAGTATTTGTACAAAATCAGGGTGGGATACTTTTGAAGCTTTACAGGATCTTTATGGTGAGCTTCTCGATAGGGCAAAGTTTAACCAAAATAAACAAATTATTTTGGATGATAAAAAGCATATTGAGTTATTGAGATTCAAATTCGAGATTCACGAGAGTTGCTCGATATTTACTCGATGTCAACTTCTTTTGCCTGGCAAGTTTCATTATATTTACACCACTGTGTTGAATTCCATTTGACTTGCCACCTGGAATACACTGTCGAATGCATGTGTTCCTGAACTTTTGTTACCTTCAGAGAAATGCTTTTCGAGAGCTGAAGGATACCTTGAACCTGCAGAGGTAAAACATAATTAATTATCAGTTGTATTATAGCAGAGCACTACCTACATAGAATATTTCTTATGCTTTTGATACTTTTTTTTTAAGTTGAAGATATAAACGTCACTTGTATGTTGTAATAGTTATATACTTAATTGAGAATATGGGTGTTTAAAGTGTGTTCTAAAACATAGAAGCATCAGCTCCAGAGTACAGTAGATGCTTTTTGTCTTGTATCAAAGCAAAAAATTGGTAAAAGTTATTGGACAAATTCTTTAAAGCGTTAAAAGGAAAATTTCATTAATCTTTTATGCACCTTTAGTATCTCTTAGTTAGATTTAGCTGTTGGGAAAGTTTTTAATAATGAACTTGAAGAGTATTCAAGGATGGGAAGAATAATGAAGATGTGTCTGACGAAACTATCATTTCAAACGCTTATTTTATAGATAATGTTAGGTGGGGCAATTTTATTTTCCCTAACGTAGTTGTACCAAACAATATTGATTATGTTGACTTCCACTGCAGGATAATGAAGATATTTTGGAGGTTTATGTCAAGTCATAGGTTTCTGATGCCCTTGACAGGGCCGCGATTTGAGGATCATTTGCATATACTATGGTTGTCCATCACCTTCCATCATTCATATTTCATACCTGTCCTGTGGATAAGTTATTGTTGCGCAACAGGCTTGTTGGGTCTCTCTTTGAGAGATTATGCTGGGAAACAGCAACATGAGGTGCACGCATTTTGAATGCTTTTCCCTGATGGATTAAGATGGAAGATAAGATTTTATTGTATCTTATTTGTTCTTATTGGAAATGTTACTGATCCAATTTGCGTTACAGGGAATGTTAATGAATATCATTTGCCATAGCAAGCAATCAACATCTGACATGGATGAAAAGAGTTGGTTGGAGTTGAGGATGAAAGAATTGATTGAGGCATGTGATGGAAATTCCTCTCTTCTGGCACAGGTGAACAAGTTGAAGGATGTTGCTGCTTAG
Protein sequence

>MS.gene77103.t1

MIDFGINWIATRSDPQSSKLQGGDISATSLSWVYAAMTHMLLRVLGRVTLQEANGHVPCLPEFSPKIRLELIKYWHLAFKGQNVHMQSIELMLCRGGPIPGPGDMVVIERPLGLLFHVSVLKYFDLCTQNFLSSWRALSLTWKLHSLSIDFLVGIEVFVQNQGGILLKLYRIFMRNAFRELKDTLNLQRIMKIFWRFMSSHRFLMPLTGPRFEDHLHILWLSITFHHSYFIPVLWISYCCATGLLGLSLRDYAGKQQHEGMLMNIICHSKQSTSDMDEKSWLELRMKELIEACDGNSSLLAQVNKLKDVAA