AlfalfaGEDB | MS.gene77298

M. sativa cultivar XinJiangDaYe / MS.gene77298

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016757 GO:0016758
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77298.t1	XP_003623218.2	91.8	437	33	1	1	437	1	434	4.50E-235	823.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77298.t1	Q66PF2	50.9	470	185	8	4	431	8	473	7.5e-130	465.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77298.t1	G7L6J2	91.8	437	33	1	1	437	1	434	3.2e-235	823.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene77298	0.801782	7.65E-49	-1.69E-46
MS.gene049378	MS.gene77298	0.820946	5.24E-53	-1.69E-46
MS.gene050226	MS.gene77298	0.810228	1.28E-50	-1.69E-46
MS.gene050227	MS.gene77298	0.822628	2.14E-53	-1.69E-46
MS.gene050228	MS.gene77298	0.826188	3.11E-54	-1.69E-46
MS.gene050330	MS.gene77298	-0.808946	2.41E-50	-1.69E-46
MS.gene050642	MS.gene77298	0.808814	2.57E-50	-1.69E-46
MS.gene052176	MS.gene77298	0.857787	1.29E-62	-1.69E-46
MS.gene052376	MS.gene77298	0.800799	1.22E-48	-1.69E-46
MS.gene052389	MS.gene77298	0.810757	9.84E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77298.t1	MTR_7g067330	91.762	437	33	1	1	437	1	434	0.0	825
MS.gene77298.t1	MTR_7g067120	87.414	437	52	1	1	437	1	434	0.0	783
MS.gene77298.t1	MTR_8g068330	42.857	462	227	7	3	429	2	461	1.26e-129	382
MS.gene77298.t1	MTR_8g068340	42.612	467	227	6	1	430	4	466	1.58e-128	380
MS.gene77298.t1	MTR_8g068360	42.241	464	228	7	1	428	4	463	1.53e-127	377
MS.gene77298.t1	MTR_6g036650	43.077	455	225	6	3	425	12	464	6.54e-122	363
MS.gene77298.t1	MTR_2g008220	41.189	454	235	6	2	425	7	458	1.54e-120	359
MS.gene77298.t1	MTR_2g008225	40.749	454	237	6	2	425	8	459	4.69e-120	358
MS.gene77298.t1	MTR_4g123553	41.667	456	229	9	6	431	12	460	2.14e-117	351
MS.gene77298.t1	MTR_2g046620	40.708	452	239	7	6	431	13	461	5.40e-115	345

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77298.t1	AT2G22590	44.880	459	211	9	7	428	13	466	1.67e-127	377
MS.gene77298.t1	AT5G65550	44.397	464	216	12	2	429	3	460	9.80e-126	372
MS.gene77298.t1	AT5G49690	43.516	455	227	8	1	429	2	452	2.77e-122	363
MS.gene77298.t1	AT5G54010	32.301	452	266	12	7	429	4	444	1.15e-66	220
MS.gene77298.t1	AT3G29630	32.151	451	271	13	7	432	4	444	3.02e-64	213
MS.gene77298.t1	AT1G64910	31.126	453	266	13	6	429	3	438	3.08e-63	211
MS.gene77298.t1	AT5G53990	29.581	453	273	14	6	429	3	438	2.74e-62	208
MS.gene77298.t1	AT1G64920	31.264	451	272	14	6	429	3	442	4.16e-62	208
MS.gene77298.t1	AT4G27570	30.501	459	280	13	7	437	5	452	2.67e-61	206
MS.gene77298.t1	AT2G22930	30.512	449	270	13	8	429	5	438	2.27e-60	203

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Find 90 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTTGCTCATTATGAAA+AGG	0.487328	7.2:+36278422	None:intergenic
AAAGAAATGATGACAATGAT+GGG	0.540780	7.2:-36278355	MS.gene77298:CDS
AAGGATTGATTTGGAGTAAT+TGG	0.116836	7.2:-36278556	MS.gene77298:CDS
AAGTCATATATAATACAATC+AGG	0.420016	7.2:+36279130	None:intergenic
AAGTGCTAATGCTTAGGAGC+AGG	0.488421	7.2:+36278167	None:intergenic
AATATTGGTGGTAACCAATA+AGG	0.329461	7.2:+36279103	None:intergenic
AATGCTTAGGAGCAGGGATA+TGG	0.332507	7.2:+36278174	None:intergenic
AATTGGTATGTCTTATGGAG+TGG	0.685913	7.2:-36279035	MS.gene77298:CDS
ACAATGATGGGAAATTCACA+AGG	0.639422	7.2:-36278343	MS.gene77298:CDS
ACAGCAACGTGAAGTTTCTT+AGG	0.355812	7.2:+36279451	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACCTTGCTCATTATGAAA+AGG	+	chr7.2:36279216-36279235	None:intergenic	30.0%
AAGAAAAATGTGATTCCAGT+TGG	-	chr7.2:36278808-36278827	MS.gene77298:CDS	30.0%
AGTTTAGAAAGCTCAAAGAA+TGG	+	chr7.2:36278241-36278260	None:intergenic	30.0%
ATTGGGATACAATCCTTAAA+TGG	-	chr7.2:36278878-36278897	MS.gene77298:CDS	30.0%
CAAAGAAATGATGACAATGA+TGG	-	chr7.2:36279279-36279298	MS.gene77298:CDS	30.0%
CAATAAAATCAGCACCAAAA+AGG	+	chr7.2:36278729-36278748	None:intergenic	30.0%
CATGTTTATCCAACCATTTA+AGG	+	chr7.2:36278894-36278913	None:intergenic	30.0%
GAGAACCAATCAAATAAGAA+AGG	-	chr7.2:36279060-36279079	MS.gene77298:CDS	30.0%
GGTTGGATAAACATGAAAAA+AGG	-	chr7.2:36278899-36278918	MS.gene77298:CDS	30.0%
GTAAATTTGGAGGTAGTTTA+GGG	+	chr7.2:36278324-36278343	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	36278172	36279485	36278172	ID=MS.gene77298
chr7.2	mRNA	36278172	36279485	36278172	ID=MS.gene77298.t1;Parent=MS.gene77298
chr7.2	exon	36278172	36279485	36278172	ID=MS.gene77298.t1.exon1;Parent=MS.gene77298.t1
chr7.2	CDS	36278172	36279485	36278172	ID=cds.MS.gene77298.t1;Parent=MS.gene77298.t1

Gene Sequence

>MS.gene77298

ATGGAAGATAAGCCTAAGAAACTTCACGTTGCTGTTTTTCCATGGCTAGCTTTTGGTCACATAAGTCCATTCTTTGAGCTTTCTAAACTCATAGCTCAAAAGGGTCACAAAATTTCATTCATTTCCACACCTAGAAACATCAAACGTCTCCCTAAACTACCTCCAAATTTACAACCTTTGGTAAATTTTGTAGAACTCTCACTTCCTCATATAGACCAACTACCTGAGCATGCAGAAGCCACTATGGACATTCCAACACACGTTGTTCCATATCTCAAGAAAGCTTTTGATGGTCTTCAACAACCTTTGATTGAATTTTTGGAAAAATCCAACCCTGATTGTATTATATATGACTTTGCACCTTATTGGTTACCACCAATATTACCAAAGTTTGGCATCTTAAGCATTTATTTCTCAATTTTTAGTGCAATTGGTATGTCTTATGGAGTGGAATTCTTAGTTGGAAAGTCAAATGATGAGGAGAATATTATTTCTGATGTCTACTTAGAACAAAATGAATCTGGAGTTACAGATATGTTTAGGGTCAAAGAAACCCTTTTTGGTGCTGATTTTATTGCTGCAAGAAGTTGCATGGAGGTTGAAGGTAAATATCTAGAATTAATTGAAAATCTATGCAAGAAAAATGTGATTCCAGTTGGATTATTGCCACCTTCACTACAATTCAATGAAGACAAAAACGATGAAAATTGGGATACAATCCTTAAATGGTTGGATAAACATGAAAAAAGGTCAGTGGTTTATGTTGCATTTGGAAGTGAAGTGACACTAAGTGATGAAGAATTCACTGAGATAGCTAAAGGATTAGAATTATCTAGTTTTCCCTTTCTTTGGATTCTGAAGAACCAAGTTAAAGATGGTTGGCTTGTTGAGAACCAATCAAATAAGAAAGGATTGATTTGGAGTAATTGGGCACCACAGTTGAGAATCTTGTCACATGAGTCCATTGGAGGATTTTTGACTCATTGTGGTTGGAGTTCAGTGATAGAAAGTCTTCAAGTTGGATGTCCACTCATTATGTTACCTTTTCATAATGAGCAAGGTTTAGTTGCTAGACATATGGAAGAAAGAATGGTGGGGGTAAAGGTACAAAGAAATGATGACAATGATGGGAAATTCACAAGGGATTCAGTGGCTAAGGCATTGAGATCAGTGATGGTAGGGGAGGAAGGAGAGAGTTATAGAAATAAAGCTGTAGAGATGAGCAAGATATTTGGAGACAAGGAGCTGCACCAAAAATATTTAGATGACTTTGTTGATTATGTGGAGCTCCATATCCCTGCTCCTAAGCATTAG

Protein sequence

>MS.gene77298.t1

MEDKPKKLHVAVFPWLAFGHISPFFELSKLIAQKGHKISFISTPRNIKRLPKLPPNLQPLVNFVELSLPHIDQLPEHAEATMDIPTHVVPYLKKAFDGLQQPLIEFLEKSNPDCIIYDFAPYWLPPILPKFGILSIYFSIFSAIGMSYGVEFLVGKSNDEENIISDVYLEQNESGVTDMFRVKETLFGADFIAARSCMEVEGKYLELIENLCKKNVIPVGLLPPSLQFNEDKNDENWDTILKWLDKHEKRSVVYVAFGSEVTLSDEEFTEIAKGLELSSFPFLWILKNQVKDGWLVENQSNKKGLIWSNWAPQLRILSHESIGGFLTHCGWSSVIESLQVGCPLIMLPFHNEQGLVARHMEERMVGVKVQRNDDNDGKFTRDSVAKALRSVMVGEEGESYRNKAVEMSKIFGDKELHQKYLDDFVDYVELHIPAPKH