Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77825.t1 | XP_013456093.1 | 97.3 | 262 | 7 | 0 | 1 | 262 | 1 | 262 | 2.60E-143 | 518.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77825.t1 | Q8L727 | 35.7 | 196 | 112 | 5 | 46 | 228 | 267 | 461 | 1.6e-21 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77825.t1 | A0A072UK42 | 97.3 | 262 | 7 | 0 | 1 | 262 | 1 | 262 | 1.9e-143 | 518.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77825 | MS.gene90825 | PPI |
| MS.gene77825 | MS.gene97346 | PPI |
| MS.gene77825 | MS.gene029093 | PPI |
| MS.gene77825 | MS.gene066439 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77825.t1 | MTR_4g063670 | 97.509 | 281 | 7 | 0 | 1 | 281 | 1 | 281 | 0.0 | 575 |
| MS.gene77825.t1 | MTR_1g051185 | 85.409 | 281 | 16 | 1 | 1 | 281 | 1 | 256 | 3.20e-165 | 459 |
| MS.gene77825.t1 | MTR_0867s0010 | 69.395 | 281 | 86 | 0 | 1 | 281 | 1 | 281 | 1.88e-128 | 368 |
| MS.gene77825.t1 | MTR_4g083510 | 65.836 | 281 | 95 | 1 | 1 | 280 | 1 | 281 | 4.09e-126 | 362 |
| MS.gene77825.t1 | MTR_3g069460 | 83.908 | 174 | 28 | 0 | 1 | 174 | 1 | 174 | 8.20e-109 | 312 |
| MS.gene77825.t1 | MTR_8g015750 | 62.805 | 164 | 45 | 2 | 118 | 281 | 1 | 148 | 1.34e-53 | 172 |
| MS.gene77825.t1 | MTR_7g088160 | 44.907 | 216 | 110 | 5 | 21 | 231 | 76 | 287 | 5.35e-49 | 167 |
| MS.gene77825.t1 | MTR_7g107340 | 41.121 | 214 | 100 | 7 | 6 | 214 | 54 | 246 | 7.13e-35 | 128 |
| MS.gene77825.t1 | MTR_8g015740 | 62.195 | 82 | 31 | 0 | 1 | 82 | 49 | 130 | 2.21e-31 | 114 |
| MS.gene77825.t1 | MTR_7g114170 | 38.150 | 173 | 71 | 6 | 18 | 190 | 61 | 197 | 3.37e-21 | 90.9 |
| MS.gene77825.t1 | MTR_6g081150 | 33.333 | 204 | 72 | 7 | 19 | 222 | 16 | 155 | 1.15e-20 | 87.0 |
| MS.gene77825.t1 | MTR_2g023560 | 61.905 | 63 | 24 | 0 | 45 | 107 | 56 | 118 | 1.06e-19 | 82.8 |
| MS.gene77825.t1 | MTR_4g013220 | 27.907 | 172 | 113 | 4 | 36 | 198 | 69 | 238 | 1.33e-16 | 79.0 |
| MS.gene77825.t1 | MTR_6g453050 | 26.667 | 195 | 126 | 5 | 9 | 191 | 45 | 234 | 2.33e-16 | 78.2 |
| MS.gene77825.t1 | MTR_3g067785 | 26.344 | 186 | 126 | 4 | 22 | 198 | 55 | 238 | 9.09e-16 | 76.6 |
| MS.gene77825.t1 | MTR_4g026120 | 27.326 | 172 | 114 | 4 | 36 | 198 | 69 | 238 | 9.63e-16 | 76.3 |
| MS.gene77825.t1 | MTR_4g036435 | 27.326 | 172 | 114 | 4 | 36 | 198 | 69 | 238 | 9.63e-16 | 76.3 |
| MS.gene77825.t1 | MTR_5g066280 | 26.344 | 186 | 126 | 4 | 22 | 198 | 55 | 238 | 1.03e-15 | 76.3 |
| MS.gene77825.t1 | MTR_5g077245 | 28.488 | 172 | 112 | 5 | 36 | 198 | 122 | 291 | 2.10e-15 | 75.9 |
| MS.gene77825.t1 | MTR_2g022120 | 26.829 | 205 | 126 | 6 | 22 | 217 | 119 | 308 | 2.45e-15 | 75.9 |
| MS.gene77825.t1 | MTR_1g106885 | 26.829 | 205 | 126 | 6 | 22 | 217 | 116 | 305 | 2.66e-15 | 75.5 |
| MS.gene77825.t1 | MTR_7g062380 | 26.744 | 172 | 115 | 5 | 36 | 198 | 4 | 173 | 4.40e-15 | 73.6 |
| MS.gene77825.t1 | MTR_7g461350 | 27.011 | 174 | 116 | 5 | 7 | 174 | 85 | 253 | 7.84e-15 | 73.9 |
| MS.gene77825.t1 | MTR_6g016670 | 27.011 | 174 | 116 | 5 | 7 | 174 | 85 | 253 | 7.84e-15 | 73.9 |
| MS.gene77825.t1 | MTR_3g464310 | 27.011 | 174 | 116 | 5 | 7 | 174 | 85 | 253 | 7.84e-15 | 73.9 |
| MS.gene77825.t1 | MTR_2g072860 | 26.437 | 174 | 117 | 5 | 7 | 174 | 17 | 185 | 8.61e-15 | 73.6 |
| MS.gene77825.t1 | MTR_6g092533 | 28.302 | 159 | 106 | 4 | 22 | 174 | 119 | 275 | 1.50e-14 | 73.2 |
| MS.gene77825.t1 | MTR_3g464170 | 26.437 | 174 | 117 | 5 | 7 | 174 | 85 | 253 | 1.69e-14 | 73.2 |
| MS.gene77825.t1 | MTR_1g100375 | 26.437 | 174 | 117 | 5 | 7 | 174 | 85 | 253 | 1.69e-14 | 73.2 |
| MS.gene77825.t1 | MTR_3g450670 | 25.926 | 162 | 110 | 4 | 19 | 174 | 92 | 249 | 1.82e-14 | 73.2 |
| MS.gene77825.t1 | MTR_1g471070 | 25.926 | 162 | 110 | 4 | 19 | 174 | 92 | 249 | 1.82e-14 | 73.2 |
| MS.gene77825.t1 | MTR_6g051405 | 28.448 | 116 | 81 | 2 | 59 | 174 | 140 | 253 | 2.87e-14 | 72.4 |
| MS.gene77825.t1 | MTR_7g083520 | 28.000 | 125 | 88 | 2 | 46 | 170 | 59 | 181 | 3.99e-14 | 71.2 |
| MS.gene77825.t1 | MTR_1g086750 | 29.286 | 140 | 91 | 3 | 38 | 171 | 6 | 143 | 3.98e-13 | 67.4 |
| MS.gene77825.t1 | MTR_4g046090 | 27.273 | 132 | 91 | 3 | 68 | 196 | 67 | 196 | 1.41e-11 | 63.5 |
| MS.gene77825.t1 | MTR_5g044920 | 75.000 | 36 | 9 | 0 | 47 | 82 | 21 | 56 | 1.52e-11 | 60.1 |
| MS.gene77825.t1 | MTR_6g069240 | 25.658 | 152 | 101 | 4 | 71 | 222 | 19 | 158 | 4.63e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77825.t1 | AT3G47680 | 36.667 | 210 | 119 | 7 | 30 | 231 | 23 | 226 | 2.16e-27 | 108 |
| MS.gene77825.t1 | AT5G41240 | 36.842 | 190 | 106 | 6 | 46 | 222 | 267 | 455 | 3.35e-27 | 110 |
| MS.gene77825.t1 | AT5G41220 | 39.227 | 181 | 93 | 7 | 56 | 222 | 219 | 396 | 2.18e-26 | 108 |
| MS.gene77825.t1 | AT5G41220 | 39.227 | 181 | 93 | 7 | 56 | 222 | 227 | 404 | 2.37e-26 | 108 |
| MS.gene77825.t1 | AT5G41220 | 39.227 | 181 | 93 | 7 | 56 | 222 | 277 | 454 | 2.45e-26 | 108 |
| MS.gene77825.t1 | AT1G66235 | 25.185 | 270 | 152 | 7 | 56 | 276 | 11 | 279 | 4.80e-13 | 68.2 |
Find 0 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTCAAAGTATCAAATT+TGG | - | 32553:8871-8890 | MS.gene77825:intergenic | 15.0% |
| !! | ATAAATCTTGATGAAGAAAT+TGG | + | 32553:8273-8292 | MS.gene77825:CDS | 20.0% |
| !! | ATCAAAAAGGAGATTGTTTT+TGG | + | 32553:8403-8422 | MS.gene77825:CDS | 25.0% |
| !! | CTTCTGTTAATAAGTTTGTT+GGG | + | 32553:8523-8542 | MS.gene77825:CDS | 25.0% |
| !!! | TTCTTTGTTCTTTTTGAAGA+AGG | - | 32553:8714-8733 | MS.gene77825:intergenic | 25.0% |
| !!! | TTTGGTTGGAATCTTATTTT+CGG | - | 32553:8321-8340 | MS.gene77825:intergenic | 25.0% |
| AGAACAAAGAATTCAACTTC+TGG | + | 32553:8723-8742 | MS.gene77825:CDS | 30.0% | |
| ATCATGGTTCAACATTTCAA+AGG | + | 32553:8362-8381 | MS.gene77825:CDS | 30.0% | |
| ATGAACTTCTCATTCAATCA+TGG | + | 32553:8346-8365 | MS.gene77825:CDS | 30.0% | |
| CCAACAAACTTATTAACAGA+AGG | - | 32553:8525-8544 | MS.gene77825:intergenic | 30.0% | |
| CCTTCTGTTAATAAGTTTGT+TGG | + | 32553:8522-8541 | MS.gene77825:CDS | 30.0% | |
| CGTAGAAAATTCACATTTGA+GGG | + | 32553:8636-8655 | MS.gene77825:CDS | 30.0% | |
| ! | ATTGTTGGAGTTGATCAAAA+AGG | + | 32553:8390-8409 | MS.gene77825:CDS | 30.0% |
| !!! | ACCACTTTTTTTCAAACCAA+CGG | - | 32553:8562-8581 | MS.gene77825:intergenic | 30.0% |
| !!! | TCTCTGGACAATTTTTTTCA+CGG | - | 32553:8458-8477 | MS.gene77825:intergenic | 30.0% |
| AGGAATTTCATGGCACTAAA+AGG | + | 32553:8477-8496 | MS.gene77825:CDS | 35.0% | |
| ATGAGAAGTTCATCATCGTT+TGG | - | 32553:8339-8358 | MS.gene77825:intergenic | 35.0% | |
| ATGTCTCATGAGGTTCATAT+TGG | + | 32553:8177-8196 | MS.gene77825:CDS | 35.0% | |
| ATTTCAAAGGACTCGATTGT+TGG | + | 32553:8375-8394 | MS.gene77825:CDS | 35.0% | |
| CATGAGGTTCATATTGGTAA+TGG | + | 32553:8183-8202 | MS.gene77825:CDS | 35.0% | |
| GCAAAGAACCGAAAATGATA+GGG | + | 32553:8995-9014 | MS.gene77825:CDS | 35.0% | |
| GCGTAGAAAATTCACATTTG+AGG | + | 32553:8635-8654 | MS.gene77825:CDS | 35.0% | |
| GTGAAAAAAATTGTCCAGAG+AGG | + | 32553:8457-8476 | MS.gene77825:CDS | 35.0% | |
| TAATGAAGGATGAACCCAAA+TGG | + | 32553:8670-8689 | MS.gene77825:CDS | 35.0% | |
| TGCAAAGAACCGAAAATGAT+AGG | + | 32553:8994-9013 | MS.gene77825:CDS | 35.0% | |
| !!! | ATTTTCGGTGTTTTTACAGC+CGG | - | 32553:8306-8325 | MS.gene77825:intergenic | 35.0% |
| AATGAACCTGCACTCCATTT+GGG | - | 32553:8687-8706 | None:intergenic | 40.0% | |
| ACACCAAAATCTTGCACTCA+AGG | + | 32553:8237-8256 | MS.gene77825:CDS | 40.0% | |
| AGCCAAAATAATGTCGCTCT+CGG | - | 32553:8589-8608 | MS.gene77825:intergenic | 40.0% | |
| GAAGTTCATCATCGTTTGGT+TGG | - | 32553:8335-8354 | MS.gene77825:intergenic | 40.0% | |
| TAATGAACCTGCACTCCATT+TGG | - | 32553:8688-8707 | MS.gene77825:intergenic | 40.0% | |
| TGAAGAAATTGGATCTGCAC+CGG | + | 32553:8284-8303 | MS.gene77825:CDS | 40.0% | |
| TGCACGTATTGAGAGTGAAA+AGG | + | 32553:8938-8957 | MS.gene77825:CDS | 40.0% | |
| TGCATAATCACGTGCAAACA+CGG | - | 32553:8922-8941 | MS.gene77825:intergenic | 40.0% | |
| TTGTCCAGAGAGGAATTTCA+TGG | + | 32553:8467-8486 | MS.gene77825:CDS | 40.0% | |
| ! | AATTCACATTTGAGGGTGTG+TGG | + | 32553:8643-8662 | MS.gene77825:CDS | 40.0% |
| ! | TGAATTTTCTACGCATGTCG+TGG | - | 32553:8629-8648 | MS.gene77825:intergenic | 40.0% |
| ! | TTGTATTCGCTTGTTGGTGT+TGG | - | 32553:8774-8793 | MS.gene77825:intergenic | 40.0% |
| !! | GAGGTTCATATTGGTAATGG+TGG | + | 32553:8186-8205 | MS.gene77825:CDS | 40.0% |
| !! | GCCGTTGGTTTGAAAAAAAG+TGG | + | 32553:8558-8577 | MS.gene77825:CDS | 40.0% |
| AGGTCGAGATAGAGAGGAAA+AGG | + | 32553:8958-8977 | MS.gene77825:CDS | 45.0% | |
| AGTGCCATGAAATTCCTCTC+TGG | - | 32553:8474-8493 | MS.gene77825:intergenic | 45.0% | |
| CCAAAATCTTGCACTCAAGG+TGG | + | 32553:8240-8259 | MS.gene77825:CDS | 45.0% | |
| GGGTGTGTGGAGATTAATGA+AGG | + | 32553:8656-8675 | MS.gene77825:CDS | 45.0% | |
| GGTCGAGATAGAGAGGAAAA+GGG | + | 32553:8959-8978 | MS.gene77825:CDS | 45.0% | |
| GTGAAAAGGTCGAGATAGAG+AGG | + | 32553:8952-8971 | MS.gene77825:CDS | 45.0% | |
| TCAAAAGGCGGCTAAAAGGA+AGG | + | 32553:8815-8834 | MS.gene77825:CDS | 45.0% | |
| TCATGGCACTAAAAGGTCGA+TGG | + | 32553:8484-8503 | MS.gene77825:CDS | 45.0% | |
| ! | CCACCTTGAGTGCAAGATTT+TGG | - | 32553:8243-8262 | MS.gene77825:intergenic | 45.0% |
| ! | CTCCGAGAGCGACATTATTT+TGG | + | 32553:8584-8603 | MS.gene77825:CDS | 45.0% |
| ! | TTTTGACCGATTGGACGCTT+CGG | - | 32553:8801-8820 | MS.gene77825:intergenic | 45.0% |
| !! | AGAGAGGAAAAGGGTTGATG+CGG | + | 32553:8968-8987 | MS.gene77825:CDS | 45.0% |
| !! | TATTCGCTTGTTGGTGTTGG+TGG | - | 32553:8771-8790 | MS.gene77825:intergenic | 45.0% |
| !! | TGTTGGTGTTGGTGGATTAG+AGG | - | 32553:8763-8782 | MS.gene77825:intergenic | 45.0% |
| ATCTTGCACTCAAGGTGGCT+TGG | + | 32553:8245-8264 | MS.gene77825:CDS | 50.0% | |
| GATGAACCCAAATGGAGTGC+AGG | + | 32553:8678-8697 | MS.gene77825:CDS | 50.0% | |
| TCGGTCAAAAGGCGGCTAAA+AGG | + | 32553:8811-8830 | MS.gene77825:CDS | 50.0% | |
| ! | GAAGCGTCCAATCGGTCAAA+AGG | + | 32553:8800-8819 | MS.gene77825:CDS | 50.0% |
| ! | TTAGCCGCCTTTTGACCGAT+TGG | - | 32553:8810-8829 | MS.gene77825:intergenic | 50.0% |
| !! | GGCGGATTGTATTCGCTTGT+TGG | - | 32553:8780-8799 | MS.gene77825:intergenic | 50.0% |
| !! | GGTGTTGGTGGATTAGAGGA+CGG | - | 32553:8759-8778 | MS.gene77825:intergenic | 50.0% |
| GCGTCCAATCGGTCAAAAGG+CGG | + | 32553:8803-8822 | MS.gene77825:CDS | 55.0% | |
| ! | AATCCGCCGAAGCGTCCAAT+CGG | + | 32553:8792-8811 | MS.gene77825:CDS | 55.0% |
| ! | GGGTGCTACAAACAAGCCGT+TGG | + | 32553:8543-8562 | MS.gene77825:CDS | 55.0% |
| TGACCGATTGGACGCTTCGG+CGG | - | 32553:8798-8817 | MS.gene77825:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32553 | gene | 8177 | 9021 | 8177 | ID=MS.gene77825 |
| 32553 | mRNA | 8177 | 9021 | 8177 | ID=MS.gene77825.t1;Parent=MS.gene77825 |
| 32553 | exon | 8177 | 9021 | 8177 | ID=MS.gene77825.t1.exon1;Parent=MS.gene77825.t1 |
| 32553 | CDS | 8177 | 9021 | 8177 | ID=cds.MS.gene77825.t1;Parent=MS.gene77825.t1 |
| Gene Sequence |
| Protein sequence |
