Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77896.t1 | XP_003599380.1 | 99.1 | 340 | 3 | 0 | 1 | 340 | 1 | 340 | 1.30E-197 | 698.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77896.t1 | Q9LM02 | 86.0 | 335 | 47 | 0 | 1 | 335 | 1 | 335 | 4.1e-176 | 618.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77896.t1 | B7FHU0 | 99.1 | 340 | 3 | 0 | 1 | 340 | 1 | 340 | 9.4e-198 | 698.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77896 | MS.gene064797 | PPI |
| MS.gene014381 | MS.gene77896 | PPI |
| MS.gene69360 | MS.gene77896 | PPI |
| MS.gene77896 | MS.gene52804 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77896.t1 | MTR_3g032530 | 99.118 | 340 | 3 | 0 | 1 | 340 | 1 | 340 | 0.0 | 706 |
| MS.gene77896.t1 | MTR_3g032530 | 99.327 | 297 | 2 | 0 | 44 | 340 | 1 | 297 | 0.0 | 622 |
| MS.gene77896.t1 | MTR_3g114780 | 41.369 | 336 | 179 | 4 | 8 | 340 | 39 | 359 | 1.69e-87 | 267 |
| MS.gene77896.t1 | MTR_7g012060 | 53.933 | 89 | 12 | 2 | 148 | 207 | 65 | 153 | 5.28e-23 | 94.0 |
| MS.gene77896.t1 | MTR_1g014690 | 26.882 | 186 | 125 | 3 | 45 | 220 | 69 | 253 | 1.28e-13 | 71.2 |
| MS.gene77896.t1 | MTR_6g069600 | 30.178 | 169 | 105 | 4 | 33 | 201 | 238 | 393 | 1.38e-13 | 72.0 |
| MS.gene77896.t1 | MTR_1g014690 | 30.400 | 125 | 86 | 1 | 96 | 220 | 75 | 198 | 4.11e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77896.t1 | AT5G13710 | 85.970 | 335 | 47 | 0 | 1 | 335 | 1 | 335 | 0.0 | 624 |
| MS.gene77896.t1 | AT5G13710 | 85.970 | 335 | 47 | 0 | 1 | 335 | 1 | 335 | 0.0 | 624 |
| MS.gene77896.t1 | AT1G20330 | 42.216 | 334 | 173 | 5 | 8 | 337 | 39 | 356 | 1.33e-89 | 273 |
| MS.gene77896.t1 | AT1G76090 | 40.882 | 340 | 182 | 5 | 4 | 339 | 34 | 358 | 1.18e-86 | 265 |
| MS.gene77896.t1 | AT1G48600 | 31.361 | 169 | 106 | 5 | 83 | 251 | 256 | 414 | 1.13e-13 | 72.0 |
| MS.gene77896.t1 | AT1G48600 | 31.361 | 169 | 106 | 5 | 83 | 251 | 272 | 430 | 1.15e-13 | 72.0 |
| MS.gene77896.t1 | AT1G73600 | 35.088 | 114 | 72 | 1 | 90 | 203 | 277 | 388 | 1.49e-13 | 71.6 |
| MS.gene77896.t1 | AT1G73600 | 35.088 | 114 | 72 | 1 | 90 | 203 | 291 | 402 | 1.56e-13 | 71.6 |
| MS.gene77896.t1 | AT3G18000 | 31.288 | 163 | 103 | 4 | 89 | 251 | 277 | 430 | 1.76e-13 | 71.6 |
| MS.gene77896.t1 | AT1G64970 | 34.615 | 104 | 68 | 0 | 98 | 201 | 130 | 233 | 5.60e-13 | 69.3 |
Find 77 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATGTTGGTTGTGGTATT+GGG | 0.139062 | 3.4:-24793092 | MS.gene77896:CDS |
| AATCGAGAAATTTCTCTTAA+TGG | 0.184007 | 3.4:+24793066 | None:intergenic |
| TTGGATGTTGGTTGTGGTAT+TGG | 0.186951 | 3.4:-24793093 | MS.gene77896:CDS |
| CAGAAGCAATACCATATTTC+TGG | 0.200578 | 3.4:+24789747 | None:intergenic |
| TAAGAGAAATTTCTCGATTC+AGG | 0.202299 | 3.4:-24793062 | MS.gene77896:intron |
| TGCAGCTCGACATCTGTTAC+AGG | 0.301259 | 3.4:-24792596 | MS.gene77896:intron |
| ATGGACACCTTGTTGCAAAA+TGG | 0.313547 | 3.4:+24793387 | None:intergenic |
| TCTTCCTTGGTACTTGCCTT+TGG | 0.316688 | 3.4:-24789827 | MS.gene77896:CDS |
| CAGGCACTCAATCGCAAAAC+TGG | 0.331030 | 3.4:-24792176 | MS.gene77896:CDS |
| CACTTCCTTGCTTTACAACT+TGG | 0.357247 | 3.4:-24793245 | MS.gene77896:CDS |
| GAAAGCTAATTATAGTGATA+TGG | 0.367025 | 3.4:-24794128 | MS.gene77896:intron |
| GGATGTTGGTTGTGGTATTG+GGG | 0.388964 | 3.4:-24793091 | MS.gene77896:CDS |
| GTGAAGGCTCTGGAGTTTGT+TGG | 0.407221 | 3.4:-24789424 | MS.gene77896:CDS |
| TAGAGCTTCAAGACACTTTG+TGG | 0.411789 | 3.4:+24790474 | None:intergenic |
| ATTCAGTTGCTTTCTGAAGC+AGG | 0.422845 | 3.4:+24788482 | None:intergenic |
| ATACCTTTCCCCGGGTGATC+TGG | 0.426976 | 3.4:+24792555 | None:intergenic |
| AATATTTCACATACAGCATC+TGG | 0.437219 | 3.4:+24791007 | None:intergenic |
| ATGGATTTGGCTTCGAATCT+CGG | 0.442435 | 3.4:-24796353 | MS.gene77896:CDS |
| TGAACCCTTTGACTCCCCTT+CGG | 0.452704 | 3.4:+24789394 | None:intergenic |
| AAAGATGAAAACCTTCTGTC+CGG | 0.459180 | 3.4:+24793214 | None:intergenic |
| CATCGGACTCTCCTCTTCCT+TGG | 0.478580 | 3.4:-24789840 | MS.gene77896:CDS |
| TCTACCGATGCAGATGGGTA+GGG | 0.478854 | 3.4:-24793301 | MS.gene77896:intron |
| ATACCATATTTCTGGTGAAA+AGG | 0.482576 | 3.4:+24789755 | None:intergenic |
| CTGCAGGCAGAAATTGAGAT+TGG | 0.490804 | 3.4:-24790523 | MS.gene77896:intron |
| GCTGCAGAGGGTCTAGTTGA+AGG | 0.505379 | 3.4:-24789355 | MS.gene77896:CDS |
| ACATTTGTAGGTGAAGGCTC+TGG | 0.507945 | 3.4:-24789434 | MS.gene77896:intron |
| GAGAAGTATCATGCATCCTA+TGG | 0.508363 | 3.4:-24794169 | MS.gene77896:CDS |
| TTGCACCGAAGGGGAGTCAA+AGG | 0.512975 | 3.4:-24789399 | MS.gene77896:CDS |
| TTTGTATGTGAAGGTCATAT+GGG | 0.514447 | 3.4:-24789875 | MS.gene77896:intron |
| AGCTTCCGTCTAACGGCTGT+TGG | 0.514924 | 3.4:-24789781 | MS.gene77896:CDS |
| AAAGGCAAGTACCAAGGAAG+AGG | 0.518855 | 3.4:+24789829 | None:intergenic |
| TACAGCATCTGGTGCATGGC+AGG | 0.521675 | 3.4:+24791018 | None:intergenic |
| TAAGCAGCAAAACATTGGCC+AGG | 0.524306 | 3.4:+24790683 | None:intergenic |
| GCAGAAATTGAGATTGGTGA+TGG | 0.534551 | 3.4:-24790517 | MS.gene77896:CDS |
| CTACCGATGCAGATGGGTAG+GGG | 0.535056 | 3.4:-24793300 | MS.gene77896:intron |
| GGTTCAAGATTTCCTTGAGA+AGG | 0.535966 | 3.4:-24789377 | MS.gene77896:CDS |
| GACCCTCTGCAGCCTTCTCA+AGG | 0.541641 | 3.4:+24789365 | None:intergenic |
| TTTGTGGTCAATCTAATGTC+AGG | 0.541951 | 3.4:+24790490 | None:intergenic |
| ACTTAGCAGCTTCCGTCTAA+CGG | 0.542702 | 3.4:-24789788 | MS.gene77896:CDS |
| TTCATCCTCTTGACCTCCAT+AGG | 0.544369 | 3.4:+24794153 | None:intergenic |
| GGCGTCCAACAGCCGTTAGA+CGG | 0.545051 | 3.4:+24789776 | None:intergenic |
| CTTGAAGCTCTAAAGCAAGC+AGG | 0.547652 | 3.4:-24790463 | MS.gene77896:CDS |
| TGGTCTTAAGCCCGGACAGA+AGG | 0.551005 | 3.4:-24793225 | MS.gene77896:intron |
| TTACAACTTGGTCTTAAGCC+CGG | 0.554828 | 3.4:-24793233 | MS.gene77896:CDS |
| TTCCTTGAGAAGGCTGCAGA+GGG | 0.556231 | 3.4:-24789367 | MS.gene77896:CDS |
| AGATTCGAAGCCAAATCCAT+TGG | 0.556251 | 3.4:+24796356 | None:intergenic |
| AAGATGAAAACCTTCTGTCC+GGG | 0.556269 | 3.4:+24793215 | None:intergenic |
| TTCTACCGATGCAGATGGGT+AGG | 0.559636 | 3.4:-24793302 | MS.gene77896:intron |
| ATTCATAAGCAGCAAAACAT+TGG | 0.559750 | 3.4:+24790678 | None:intergenic |
| GTTTGTTGGACTTGCACCGA+AGG | 0.560690 | 3.4:-24789410 | MS.gene77896:CDS |
| TCAAAACACATACCTTTCCC+CGG | 0.560769 | 3.4:+24792546 | None:intergenic |
| ATGGAGGTCAAGAGGATGAA+AGG | 0.562038 | 3.4:-24794150 | MS.gene77896:CDS |
| GTACCAGATCACCCGGGGAA+AGG | 0.569425 | 3.4:-24792558 | MS.gene77896:intron |
| CACATACAGCATCTGGTGCA+TGG | 0.575106 | 3.4:+24791014 | None:intergenic |
| TCAAAACTATTGTCTTCGAA+TGG | 0.577172 | 3.4:+24791064 | None:intergenic |
| CAGAAATTGAGATTGGTGAT+GGG | 0.583752 | 3.4:-24790516 | MS.gene77896:CDS |
| TTTCCTTGAGAAGGCTGCAG+AGG | 0.588645 | 3.4:-24789368 | MS.gene77896:CDS |
| ATAATGAGTACCAGATCACC+CGG | 0.593613 | 3.4:-24792565 | MS.gene77896:CDS |
| GATGTTGGTTGTGGTATTGG+GGG | 0.596492 | 3.4:-24793090 | MS.gene77896:CDS |
| TAAGACCAAGTTGTAAAGCA+AGG | 0.600321 | 3.4:+24793240 | None:intergenic |
| TTTGTTGGACTTGCACCGAA+GGG | 0.601946 | 3.4:-24789409 | MS.gene77896:CDS |
| TGCATCCTATGGAGGTCAAG+AGG | 0.610680 | 3.4:-24794158 | MS.gene77896:CDS |
| TGCACCGAAGGGGAGTCAAA+GGG | 0.614024 | 3.4:-24789398 | MS.gene77896:CDS |
| CAAAACACATACCTTTCCCC+GGG | 0.617984 | 3.4:+24792547 | None:intergenic |
| AGCATCTGGTGCATGGCAGG+TGG | 0.619604 | 3.4:+24791021 | None:intergenic |
| GAGAAGTGACTCTTGTCCAA+AGG | 0.629432 | 3.4:+24789811 | None:intergenic |
| GCAGAGGGTCTAGTTGAAGG+TGG | 0.639355 | 3.4:-24789352 | MS.gene77896:CDS |
| ATGGGAGAAAGATCTAGCAT+CGG | 0.642273 | 3.4:-24789857 | MS.gene77896:CDS |
| TTGTTGGACTTGCACCGAAG+GGG | 0.642356 | 3.4:-24789408 | MS.gene77896:CDS |
| AAGTATCATGCATCCTATGG+AGG | 0.651186 | 3.4:-24794166 | MS.gene77896:CDS |
| CAAGACTTGCAACTTCGTCA+AGG | 0.656952 | 3.4:-24792147 | MS.gene77896:intron |
| TTGTCCAAAGGCAAGTACCA+AGG | 0.664739 | 3.4:+24789823 | None:intergenic |
| ACTCAATCGCAAAACTGGAG+TGG | 0.680106 | 3.4:-24792171 | MS.gene77896:CDS |
| TAATGAGTACCAGATCACCC+GGG | 0.687827 | 3.4:-24792564 | MS.gene77896:CDS |
| GATTTGGCTTCGAATCTCGG+TGG | 0.689368 | 3.4:-24796350 | MS.gene77896:CDS |
| AATGAGTACCAGATCACCCG+GGG | 0.722625 | 3.4:-24792563 | MS.gene77896:CDS |
| AGAGACGCACTTATCGACAG+CGG | 0.752289 | 3.4:+24796301 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 24788484 | 24796375 | 24788484 | ID=MS.gene77896 |
| chr3.4 | mRNA | 24788484 | 24796375 | 24788484 | ID=MS.gene77896.t1;Parent=MS.gene77896 |
| chr3.4 | exon | 24796311 | 24796375 | 24796311 | ID=MS.gene77896.t1.exon1;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24796311 | 24796375 | 24796311 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24794129 | 24794195 | 24794129 | ID=MS.gene77896.t1.exon2;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24794129 | 24794195 | 24794129 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24793395 | 24793468 | 24793395 | ID=MS.gene77896.t1.exon3;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24793395 | 24793468 | 24793395 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24793226 | 24793310 | 24793226 | ID=MS.gene77896.t1.exon4;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24793226 | 24793310 | 24793226 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24793063 | 24793118 | 24793063 | ID=MS.gene77896.t1.exon5;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24793063 | 24793118 | 24793063 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24792559 | 24792613 | 24792559 | ID=MS.gene77896.t1.exon6;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24792559 | 24792613 | 24792559 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24792148 | 24792195 | 24792148 | ID=MS.gene77896.t1.exon7;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24792148 | 24792195 | 24792148 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24791021 | 24791104 | 24791021 | ID=MS.gene77896.t1.exon8;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24791021 | 24791104 | 24791021 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24790625 | 24790741 | 24790625 | ID=MS.gene77896.t1.exon9;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24790625 | 24790741 | 24790625 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24790456 | 24790539 | 24790456 | ID=MS.gene77896.t1.exon10;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24790456 | 24790539 | 24790456 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24789759 | 24789884 | 24789759 | ID=MS.gene77896.t1.exon11;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24789759 | 24789884 | 24789759 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24789349 | 24789446 | 24789349 | ID=MS.gene77896.t1.exon12;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24789349 | 24789446 | 24789349 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| chr3.4 | exon | 24788484 | 24788547 | 24788484 | ID=MS.gene77896.t1.exon13;Parent=MS.gene77896.t1 |
| chr3.4 | CDS | 24788484 | 24788547 | 24788484 | ID=cds.MS.gene77896.t1;Parent=MS.gene77896.t1 |
| Gene Sequence |
| Protein sequence |
