Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78309.t1 | XP_003609685.2 | 96.7 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 6.10E-41 | 176.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78309.t1 | G7JLI7 | 96.7 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 4.4e-41 | 176.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050864 | MS.gene78309 | 0.80366 | 3.13E-49 | -1.69E-46 |
| MS.gene052494 | MS.gene78309 | 0.807425 | 5.09E-50 | -1.69E-46 |
| MS.gene053083 | MS.gene78309 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene061044 | MS.gene78309 | 0.81094 | 8.98E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78309.t1 | MTR_4g120020 | 96.694 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 8.48e-78 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78309.t1 | AT3G06890 | 75.591 | 127 | 25 | 1 | 1 | 121 | 1 | 127 | 1.50e-31 | 108 |
Find 33 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCACCTTGTTGTCTTTC+TGG | 0.014264 | 8.4:+11941884 | None:intergenic |
| GTAATAGTTCCTTTCCTTCT+CGG | 0.355214 | 8.4:-11941815 | MS.gene78309:CDS |
| AGGTTCACCGGTGGAGAAAA+TGG | 0.355541 | 8.4:+11941664 | None:intergenic |
| TGGAACCGTGTTTCGATCTT+CGG | 0.370599 | 8.4:-11941569 | MS.gene78309:CDS |
| GATACTATTCATCGTGTTAG+TGG | 0.389930 | 8.4:-11941641 | MS.gene78309:CDS |
| CCTTTCCTTCTCGGAGATCA+AGG | 0.391728 | 8.4:-11941806 | MS.gene78309:CDS |
| AGCTATCACAGAAGCTATAT+CGG | 0.433454 | 8.4:-11941780 | MS.gene78309:CDS |
| CTAACACGATGAATAGTATC+AGG | 0.440753 | 8.4:+11941644 | None:intergenic |
| AGCAGTGGTGGTAGCTATAG+TGG | 0.468105 | 8.4:-11941837 | MS.gene78309:CDS |
| ATCGTGAGAAGAAGGATGAT+TGG | 0.488963 | 8.4:+11941725 | None:intergenic |
| GGAACTGAATCGTGAGAAGA+AGG | 0.507982 | 8.4:+11941717 | None:intergenic |
| GAAAGACAACAAGGTGGTGG+AGG | 0.540517 | 8.4:-11941881 | MS.gene78309:CDS |
| CGTGTTAGTGGATCACCGTT+CGG | 0.558827 | 8.4:-11941629 | MS.gene78309:CDS |
| CCTTGATCTCCGAGAAGGAA+AGG | 0.562958 | 8.4:+11941806 | None:intergenic |
| CCAGAAAGACAACAAGGTGG+TGG | 0.583317 | 8.4:-11941884 | MS.gene78309:CDS |
| ATGAATAGTATCAGGTTCAC+CGG | 0.583799 | 8.4:+11941652 | None:intergenic |
| TCGGAGCTGCTAAGTCCACT+TGG | 0.597655 | 8.4:-11941761 | MS.gene78309:CDS |
| AACCGTGTTTCGATCTTCGG+TGG | 0.599415 | 8.4:-11941566 | MS.gene78309:CDS |
| CGGTATTCTTCTAGCAGTGG+TGG | 0.606734 | 8.4:-11941849 | MS.gene78309:CDS |
| CTTCGCCTTGATCTCCGAGA+AGG | 0.615486 | 8.4:+11941801 | None:intergenic |
| AGGAAAAGAGCAACACCGAA+CGG | 0.621281 | 8.4:+11941614 | None:intergenic |
| CAGTTCCTCTCATCTGAACG+TGG | 0.622156 | 8.4:-11941701 | MS.gene78309:CDS |
| AAAGCACAAGAATGAGAACA+AGG | 0.624793 | 8.4:+11941594 | None:intergenic |
| ACGATCCACGTTCAGATGAG+AGG | 0.629269 | 8.4:+11941696 | None:intergenic |
| ACACGGTATTCTTCTAGCAG+TGG | 0.660684 | 8.4:-11941852 | MS.gene78309:CDS |
| GGTAGAAGAAGAAGACCAAG+TGG | 0.662368 | 8.4:+11941746 | None:intergenic |
| AATAGTATCAGGTTCACCGG+TGG | 0.662862 | 8.4:+11941655 | None:intergenic |
| ATGTATCCAGAAAGACAACA+AGG | 0.678373 | 8.4:-11941890 | MS.gene78309:CDS |
| CACCACCGAAGATCGAAACA+CGG | 0.699903 | 8.4:+11941564 | None:intergenic |
| GGTGGTGGAGGAGCACCACA+CGG | 0.704308 | 8.4:-11941869 | MS.gene78309:CDS |
| TTCACCGGTGGAGAAAATGG+CGG | 0.726368 | 8.4:+11941667 | None:intergenic |
| TATCCAGAAAGACAACAAGG+TGG | 0.796650 | 8.4:-11941887 | MS.gene78309:CDS |
| ACTGCTAGAAGAATACCGTG+TGG | 0.809004 | 8.4:+11941854 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCACAAGAATGAGAACA+AGG | + | chr8.4:11941846-11941865 | None:intergenic | 35.0% | |
| AGCTATCACAGAAGCTATAT+CGG | - | chr8.4:11941657-11941676 | MS.gene78309:CDS | 35.0% | |
| ATGAATAGTATCAGGTTCAC+CGG | + | chr8.4:11941788-11941807 | None:intergenic | 35.0% | |
| ATGTATCCAGAAAGACAACA+AGG | - | chr8.4:11941547-11941566 | MS.gene78309:CDS | 35.0% | |
| CTAACACGATGAATAGTATC+AGG | + | chr8.4:11941796-11941815 | None:intergenic | 35.0% | |
| GATACTATTCATCGTGTTAG+TGG | - | chr8.4:11941796-11941815 | MS.gene78309:CDS | 35.0% | |
| GTAATAGTTCCTTTCCTTCT+CGG | - | chr8.4:11941622-11941641 | MS.gene78309:CDS | 35.0% | |
| ! | TTCTCATTCTTGTGCTTTTG+TGG | - | chr8.4:11941848-11941867 | MS.gene78309:CDS | 35.0% |
| ATCGTGAGAAGAAGGATGAT+TGG | + | chr8.4:11941715-11941734 | None:intergenic | 40.0% | |
| TATCCAGAAAGACAACAAGG+TGG | - | chr8.4:11941550-11941569 | MS.gene78309:CDS | 40.0% | |
| AATAGTATCAGGTTCACCGG+TGG | + | chr8.4:11941785-11941804 | None:intergenic | 45.0% | |
| ACACGGTATTCTTCTAGCAG+TGG | - | chr8.4:11941585-11941604 | MS.gene78309:CDS | 45.0% | |
| ACTGCTAGAAGAATACCGTG+TGG | + | chr8.4:11941586-11941605 | None:intergenic | 45.0% | |
| AGGAAAAGAGCAACACCGAA+CGG | + | chr8.4:11941826-11941845 | None:intergenic | 45.0% | |
| GGAACTGAATCGTGAGAAGA+AGG | + | chr8.4:11941723-11941742 | None:intergenic | 45.0% | |
| GGTAGAAGAAGAAGACCAAG+TGG | + | chr8.4:11941694-11941713 | None:intergenic | 45.0% | |
| TGGAACCGTGTTTCGATCTT+CGG | - | chr8.4:11941868-11941887 | MS.gene78309:CDS | 45.0% | |
| AACCGTGTTTCGATCTTCGG+TGG | - | chr8.4:11941871-11941890 | MS.gene78309:CDS | 50.0% | |
| ACGATCCACGTTCAGATGAG+AGG | + | chr8.4:11941744-11941763 | None:intergenic | 50.0% | |
| AGCAGTGGTGGTAGCTATAG+TGG | - | chr8.4:11941600-11941619 | MS.gene78309:CDS | 50.0% | |
| AGGTTCACCGGTGGAGAAAA+TGG | + | chr8.4:11941776-11941795 | None:intergenic | 50.0% | |
| CACCACCGAAGATCGAAACA+CGG | + | chr8.4:11941876-11941895 | None:intergenic | 50.0% | |
| CAGTTCCTCTCATCTGAACG+TGG | - | chr8.4:11941736-11941755 | MS.gene78309:CDS | 50.0% | |
| CCAGAAAGACAACAAGGTGG+TGG | - | chr8.4:11941553-11941572 | MS.gene78309:CDS | 50.0% | |
| CCTTGATCTCCGAGAAGGAA+AGG | + | chr8.4:11941634-11941653 | None:intergenic | 50.0% | |
| CCTTTCCTTCTCGGAGATCA+AGG | - | chr8.4:11941631-11941650 | MS.gene78309:CDS | 50.0% | |
| CGGTATTCTTCTAGCAGTGG+TGG | - | chr8.4:11941588-11941607 | MS.gene78309:CDS | 50.0% | |
| TTCACCGGTGGAGAAAATGG+CGG | + | chr8.4:11941773-11941792 | None:intergenic | 50.0% | |
| ! | CCACCACCTTGTTGTCTTTC+TGG | + | chr8.4:11941556-11941575 | None:intergenic | 50.0% |
| ! | CGTGTTAGTGGATCACCGTT+CGG | - | chr8.4:11941808-11941827 | MS.gene78309:CDS | 50.0% |
| ! | GAAAGACAACAAGGTGGTGG+AGG | - | chr8.4:11941556-11941575 | MS.gene78309:CDS | 50.0% |
| !! | ATCTCCGCCATTTTCTCCAC+CGG | - | chr8.4:11941766-11941785 | MS.gene78309:CDS | 50.0% |
| CTTCGCCTTGATCTCCGAGA+AGG | + | chr8.4:11941639-11941658 | None:intergenic | 55.0% | |
| TCGGAGCTGCTAAGTCCACT+TGG | - | chr8.4:11941676-11941695 | MS.gene78309:CDS | 55.0% | |
| !! | GGTGGTGGAGGAGCACCACA+CGG | - | chr8.4:11941568-11941587 | MS.gene78309:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 11941547 | 11941912 | 11941547 | ID=MS.gene78309 |
| chr8.4 | mRNA | 11941547 | 11941912 | 11941547 | ID=MS.gene78309.t1;Parent=MS.gene78309 |
| chr8.4 | exon | 11941547 | 11941912 | 11941547 | ID=MS.gene78309.t1.exon1;Parent=MS.gene78309.t1 |
| chr8.4 | CDS | 11941547 | 11941912 | 11941547 | ID=cds.MS.gene78309.t1;Parent=MS.gene78309.t1 |
| Gene Sequence |
| Protein sequence |
