AlfalfaGEDB | MS.gene80929

M. sativa cultivar XinJiangDaYe / MS.gene80929

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80929.t1	RHN44098.1	94.3	123	7	0	1	123	619	741	2.30E-56	228

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80929.t1	Q8VZG8	44.0	91	49	2	1	90	955	1044	1.5e-14	80.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80929.t1	A0A396GUK9	94.3	123	7	0	1	123	619	741	1.7e-56	228.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051980	MS.gene80929	0.808375	3.19E-50	-1.69E-46
MS.gene052011	MS.gene80929	-0.812919	3.33E-51	-1.69E-46
MS.gene052589	MS.gene80929	0.813529	2.44E-51	-1.69E-46
MS.gene053873	MS.gene80929	0.81431	1.64E-51	-1.69E-46
MS.gene054102	MS.gene80929	0.816213	6.21E-52	-1.69E-46
MS.gene054183	MS.gene80929	0.806045	9.95E-50	-1.69E-46
MS.gene056514	MS.gene80929	0.816582	5.14E-52	-1.69E-46
MS.gene056981	MS.gene80929	0.818793	1.63E-52	-1.69E-46
MS.gene057476	MS.gene80929	0.806906	6.55E-50	-1.69E-46
MS.gene057833	MS.gene80929	0.809872	1.53E-50	-1.69E-46
MS.gene057843	MS.gene80929	0.82835	9.44E-55	-1.69E-46
MS.gene057888	MS.gene80929	0.804144	2.49E-49	-1.69E-46
MS.gene059011	MS.gene80929	0.813136	2.98E-51	-1.69E-46
MS.gene059420	MS.gene80929	0.806844	6.75E-50	-1.69E-46
MS.gene060403	MS.gene80929	0.805896	1.07E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80929.t1	MTR_7g007540	90.984	122	11	0	1	122	1	122	1.07e-78	228
MS.gene80929.t1	MTR_7g007630	91.870	123	10	0	1	123	666	788	1.40e-73	233
MS.gene80929.t1	MTR_7g407130	90.244	123	12	0	1	123	696	818	1.00e-71	229
MS.gene80929.t1	MTR_7g007620	88.618	123	14	0	1	123	620	742	1.01e-63	206
MS.gene80929.t1	MTR_7g007560	80.328	122	24	0	1	122	653	774	1.30e-62	203
MS.gene80929.t1	MTR_0491s0030	89.431	123	13	0	1	123	704	826	2.16e-62	203
MS.gene80929.t1	MTR_8g047230	89.431	123	13	0	1	123	875	997	6.42e-62	203
MS.gene80929.t1	MTR_8g047210	89.431	123	13	0	1	123	768	890	2.47e-61	201
MS.gene80929.t1	MTR_8g047220	89.431	123	13	0	1	123	831	953	3.10e-61	201
MS.gene80929.t1	MTR_7g009970	78.689	122	26	0	1	122	669	790	9.51e-60	196
MS.gene80929.t1	MTR_7g407090	82.927	123	21	0	1	123	651	773	2.37e-59	194
MS.gene80929.t1	MTR_7g010000	77.236	123	28	0	1	123	746	868	3.53e-59	195
MS.gene80929.t1	MTR_7g007580	84.211	114	18	0	10	123	3	116	4.24e-58	175
MS.gene80929.t1	MTR_7g013680	74.797	123	31	0	1	123	619	741	2.34e-55	184
MS.gene80929.t1	MTR_7g013350	65.833	120	41	0	1	120	295	414	1.20e-47	157
MS.gene80929.t1	MTR_7g010010	64.228	123	25	1	1	123	616	719	2.25e-44	153
MS.gene80929.t1	MTR_8g047160	89.286	84	9	0	1	84	531	614	2.33e-38	136
MS.gene80929.t1	MTR_7g481630	55.446	101	40	2	1	97	80	179	1.15e-27	100
MS.gene80929.t1	MTR_7g481590	55.446	101	40	2	1	97	203	302	4.18e-26	99.8
MS.gene80929.t1	MTR_5g085700	49.107	112	52	2	3	110	922	1032	4.26e-26	102
MS.gene80929.t1	MTR_7g081570	52.632	114	48	3	1	109	939	1051	1.33e-25	100
MS.gene80929.t1	MTR_1g039080	52.809	89	36	3	3	86	194	281	3.28e-25	96.7
MS.gene80929.t1	MTR_7g009940	44.037	109	61	0	3	111	700	808	4.20e-25	99.0
MS.gene80929.t1	MTR_7g081480	47.788	113	54	2	1	109	949	1060	1.33e-23	94.7
MS.gene80929.t1	MTR_1g039310	49.451	91	40	2	3	88	1051	1140	1.79e-23	94.4
MS.gene80929.t1	MTR_7g081410	47.863	117	55	3	1	112	826	941	2.08e-23	94.4
MS.gene80929.t1	MTR_7g081780	47.368	114	55	2	1	110	947	1059	2.84e-23	94.0
MS.gene80929.t1	MTR_4g037720	46.237	93	49	1	1	93	848	939	3.59e-23	93.6
MS.gene80929.t1	MTR_7g081410	48.246	114	53	3	1	109	946	1058	3.99e-23	93.6
MS.gene80929.t1	MTR_1g039240	47.475	99	43	2	1	91	1058	1155	4.96e-23	93.2
MS.gene80929.t1	MTR_1g038890	51.648	91	38	3	1	86	1033	1122	7.24e-23	92.8
MS.gene80929.t1	MTR_4g037015	44.211	95	52	1	1	95	777	870	7.98e-23	92.4
MS.gene80929.t1	MTR_7g028432	44.211	95	52	1	1	95	853	946	1.24e-22	92.0
MS.gene80929.t1	MTR_7g039330	53.333	90	39	3	3	90	1190	1278	1.28e-22	92.0
MS.gene80929.t1	MTR_7g045510	53.333	90	39	3	3	90	1092	1180	1.38e-22	92.0
MS.gene80929.t1	MTR_7g045710	51.136	88	41	2	3	90	973	1058	1.80e-22	91.7
MS.gene80929.t1	MTR_7g081720	51.579	95	41	2	1	91	964	1057	2.12e-22	91.3
MS.gene80929.t1	MTR_8g445960	43.011	93	47	2	1	88	63	154	2.69e-22	86.3
MS.gene80929.t1	MTR_8g044940	43.011	93	47	2	1	88	63	154	2.69e-22	86.3
MS.gene80929.t1	MTR_1g039220	48.485	99	42	2	1	91	1032	1129	3.38e-22	90.9
MS.gene80929.t1	MTR_0400s0040	46.465	99	44	2	1	91	1058	1155	3.65e-21	87.8
MS.gene80929.t1	MTR_0365s0030	46.465	99	44	2	1	91	1058	1155	3.65e-21	87.8
MS.gene80929.t1	MTR_7g446160	51.546	97	41	3	1	92	887	982	3.87e-21	87.8
MS.gene80929.t1	MTR_7g045860	46.667	90	45	2	3	90	985	1073	1.84e-20	85.9
MS.gene80929.t1	MTR_1g040615	45.455	99	45	2	1	91	2024	2121	4.37e-20	84.7
MS.gene80929.t1	MTR_1g040615	44.330	97	45	2	1	89	1025	1120	1.95e-19	82.8
MS.gene80929.t1	MTR_1g040525	42.424	99	48	2	1	91	767	864	4.91e-20	84.7
MS.gene80929.t1	MTR_7g096980	42.478	113	60	3	1	109	1108	1219	5.08e-20	84.7
MS.gene80929.t1	MTR_1g040545	43.434	99	47	2	1	91	907	1004	5.22e-20	84.7
MS.gene80929.t1	MTR_7g050990	47.917	96	41	3	1	88	819	913	2.09e-18	80.1
MS.gene80929.t1	MTR_0400s0030	41.667	96	50	2	1	91	791	885	5.89e-18	78.6
MS.gene80929.t1	MTR_0365s0020	41.667	96	50	2	1	91	791	885	5.89e-18	78.6
MS.gene80929.t1	MTR_7g051040	44.444	99	45	2	1	90	843	940	5.30e-17	75.9
MS.gene80929.t1	MTR_7g446190	41.463	82	42	2	1	77	815	895	8.08e-14	67.0
MS.gene80929.t1	MTR_1g040625	35.870	92	50	2	8	91	478	568	4.64e-13	64.7
MS.gene80929.t1	MTR_1g079520	36.842	95	50	3	1	86	946	1039	4.23e-12	62.0
MS.gene80929.t1	MTR_2g070020	37.634	93	50	2	1	86	987	1078	5.20e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80929.t1	AT4G08850	43.956	91	49	2	1	90	955	1044	1.14e-18	80.9
MS.gene80929.t1	AT1G35710	44.444	90	49	1	1	90	1031	1119	1.72e-18	80.5
MS.gene80929.t1	AT2G33170	37.634	93	51	1	1	86	1003	1095	4.16e-12	62.0
MS.gene80929.t1	AT2G33170	37.634	93	51	1	1	86	1003	1095	4.16e-12	62.0
MS.gene80929.t1	AT5G63930	35.484	93	52	2	1	86	919	1010	7.95e-11	58.5
MS.gene80929.t1	AT5G63930	35.484	93	52	2	1	86	983	1074	8.14e-11	58.5
MS.gene80929.t1	AT1G17230	35.484	93	52	2	1	86	977	1068	9.23e-11	58.2

Find 17 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTATAACTACGTCGTTATT+TGG	0.146856	7.3:-90086369	None:intergenic
TCACTCAAAGGAATGCTTAA+TGG	0.247563	7.3:-90086498	None:intergenic
TGTGACACACGTTTCATTGT+TGG	0.385025	7.3:-90086453	None:intergenic
AAGTGTGATGTATATAGTTT+TGG	0.441933	7.3:+90086228	MS.gene80929:CDS
CTTTAGTTGGAAGGCACCCT+GGG	0.466628	7.3:+90086268	MS.gene80929:CDS
ACTTTAGTTGGAAGGCACCC+TGG	0.486816	7.3:+90086267	MS.gene80929:CDS
GTGGCACTTGAAACTTTAGT+TGG	0.497856	7.3:+90086255	MS.gene80929:CDS
CACTTGAAACTTTAGTTGGA+AGG	0.502085	7.3:+90086259	MS.gene80929:CDS
TGCACTGAAATTTCACTCAA+AGG	0.510871	7.3:-90086510	None:intergenic
ACTACGTCGTTATTTGGAAG+TGG	0.516663	7.3:-90086363	None:intergenic
TTCATTGTTGGTCGAGAACG+AGG	0.528233	7.3:-90086441	None:intergenic
AGTGATGACAATAAATCCCC+AGG	0.580045	7.3:-90086285	None:intergenic
TGACATAATTCATGTTGCCG+TGG	0.662248	7.3:+90086392	MS.gene80929:CDS
GTGATGACAATAAATCCCCA+GGG	0.663800	7.3:-90086284	None:intergenic
TAAGCATGCAAATGCAACCA+CGG	0.665139	7.3:-90086409	None:intergenic
TATTTGGAAGTGGAAGACGC+TGG	0.701433	7.3:-90086353	None:intergenic
TTTAGTTGGAAGGCACCCTG+GGG	0.725104	7.3:+90086269	MS.gene80929:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTGTGATGTATATAGTTT+TGG	+	chr7.3:90086228-90086247	MS.gene80929:CDS	25.0%
!!!	TGTATATAGTTTTGGTGTAG+TGG	+	chr7.3:90086236-90086255	MS.gene80929:CDS	30.0%
	CACTTGAAACTTTAGTTGGA+AGG	+	chr7.3:90086259-90086278	MS.gene80929:CDS	35.0%
	CGTATAACTACGTCGTTATT+TGG	-	chr7.3:90086372-90086391	None:intergenic	35.0%
	TCACTCAAAGGAATGCTTAA+TGG	-	chr7.3:90086501-90086520	None:intergenic	35.0%
	TGCACTGAAATTTCACTCAA+AGG	-	chr7.3:90086513-90086532	None:intergenic	35.0%
!!	TGTGTCACAAAGTTTTGTCA+CGG	+	chr7.3:90086467-90086486	MS.gene80929:CDS	35.0%
	ACTACGTCGTTATTTGGAAG+TGG	-	chr7.3:90086366-90086385	None:intergenic	40.0%
	AGTGATGACAATAAATCCCC+AGG	-	chr7.3:90086288-90086307	None:intergenic	40.0%
	GTGATGACAATAAATCCCCA+GGG	-	chr7.3:90086287-90086306	None:intergenic	40.0%
	GTGGCACTTGAAACTTTAGT+TGG	+	chr7.3:90086255-90086274	MS.gene80929:CDS	40.0%
	TAAGCATGCAAATGCAACCA+CGG	-	chr7.3:90086412-90086431	None:intergenic	40.0%
	TGACATAATTCATGTTGCCG+TGG	+	chr7.3:90086392-90086411	MS.gene80929:CDS	40.0%
	TGTGACACACGTTTCATTGT+TGG	-	chr7.3:90086456-90086475	None:intergenic	40.0%
	TATTTGGAAGTGGAAGACGC+TGG	-	chr7.3:90086356-90086375	None:intergenic	45.0%
	TTCATTGTTGGTCGAGAACG+AGG	-	chr7.3:90086444-90086463	None:intergenic	45.0%
!!	ACTTTAGTTGGAAGGCACCC+TGG	+	chr7.3:90086267-90086286	MS.gene80929:CDS	50.0%
!!	CTTTAGTTGGAAGGCACCCT+GGG	+	chr7.3:90086268-90086287	MS.gene80929:CDS	50.0%
!!	TTTAGTTGGAAGGCACCCTG+GGG	+	chr7.3:90086269-90086288	MS.gene80929:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	90086213	90086584	90086213	ID=MS.gene80929
chr7.3	mRNA	90086213	90086584	90086213	ID=MS.gene80929.t1;Parent=MS.gene80929
chr7.3	exon	90086213	90086584	90086213	ID=MS.gene80929.t1.exon1;Parent=MS.gene80929.t1
chr7.3	CDS	90086213	90086584	90086213	ID=cds.MS.gene80929.t1;Parent=MS.gene80929.t1

Gene Sequence	>MS.gene80929 ATGGTTGTGAATGAAAAGTGTGATGTATATAGTTTTGGTGTAGTGGCACTTGAAACTTTAGTTGGAAGGCACCCTGGGGATTTATTGTCATCACTTCAATCAACATCTACAAGAAGTGTCAAACTATGTCAAGTATTAGACCAGCGTCTTCCACTTCCAAATAACGACGTAGTTATACGTGACATAATTCATGTTGCCGTGGTTGCATTTGCATGCTTAAATATCAATCCTCGTTCTCGACCAACAATGAAACGTGTGTCACAAAGTTTTGTCACGGAGCTCACACCATTAAGCATTCCTTTGAGTGAAATTTCAGTGCAGCAACTAATTAGTGAAGAACTCAAAGCATTATTTTATATTGCGAACCCTTGA
Protein sequence	>MS.gene80929.t1 MVVNEKCDVYSFGVVALETLVGRHPGDLLSSLQSTSTRSVKLCQVLDQRLPLPNNDVVIRDIIHVAVVAFACLNINPRSRPTMKRVSQSFVTELTPLSIPLSEISVQQLISEELKALFYIANP