AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81017


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81017.t1 MTR_3g037390 86.957 299 31 3 7 302 268 561 0.0 538
MS.gene81017.t1 MTR_3g037390 86.957 299 31 3 7 302 164 457 0.0 537
MS.gene81017.t1 MTR_4g108880 57.288 295 95 4 7 298 298 564 1.80e-111 335
MS.gene81017.t1 MTR_4g061360 68.750 48 15 0 235 282 717 764 4.79e-16 79.0
MS.gene81017.t1 MTR_7g118260 60.000 50 20 0 235 284 367 416 3.36e-14 72.8
MS.gene81017.t1 MTR_7g118260 60.000 50 20 0 235 284 451 500 3.77e-14 73.2
MS.gene81017.t1 MTR_7g118260 60.000 50 20 0 235 284 537 586 6.20e-14 72.4
MS.gene81017.t1 MTR_7g118260 60.000 50 20 0 235 284 500 549 7.68e-14 72.0
MS.gene81017.t1 MTR_1g067110 61.404 57 19 1 226 279 513 569 3.27e-13 70.1
MS.gene81017.t1 MTR_3g092780 68.889 45 14 0 239 283 631 675 3.62e-13 70.1
MS.gene81017.t1 MTR_3g092780 68.889 45 14 0 239 283 630 674 3.68e-13 70.1
MS.gene81017.t1 MTR_3g092780 68.889 45 14 0 239 283 590 634 3.76e-13 70.1
MS.gene81017.t1 MTR_3g092780 68.889 45 14 0 239 283 589 633 3.86e-13 70.1
MS.gene81017.t1 MTR_1g067110 61.404 57 19 1 226 279 678 734 4.62e-13 70.1
MS.gene81017.t1 MTR_4g093730 55.357 56 25 0 225 280 94 149 7.76e-13 68.6
MS.gene81017.t1 MTR_8g024260 66.667 45 15 0 238 282 583 627 1.95e-12 68.2
MS.gene81017.t1 MTR_3g100040 57.143 49 21 0 231 279 19 67 4.56e-12 65.5
MS.gene81017.t1 MTR_3g100040 53.704 54 25 0 226 279 118 171 5.84e-12 65.9
MS.gene81017.t1 MTR_5g066510 56.000 50 20 1 239 286 135 184 2.28e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81017.t1 AT5G61380 43.320 247 80 10 68 300 394 594 1.77e-44 160
MS.gene81017.t1 AT5G02810 63.793 58 21 0 231 288 517 574 8.69e-17 80.9
MS.gene81017.t1 AT5G02810 63.793 58 21 0 231 288 661 718 1.30e-16 80.5
MS.gene81017.t1 AT2G46670 71.111 45 13 0 239 283 132 176 2.55e-16 75.9
MS.gene81017.t1 AT2G46790 69.565 46 14 0 238 283 259 304 1.17e-15 76.3
MS.gene81017.t1 AT2G46790 69.565 46 14 0 238 283 259 304 1.17e-15 76.3
MS.gene81017.t1 AT2G46790 69.565 46 14 0 238 283 299 344 2.33e-15 75.9
MS.gene81017.t1 AT2G46790 69.565 46 14 0 238 283 416 461 3.29e-15 76.3
MS.gene81017.t1 AT5G24470 55.357 56 25 0 227 282 497 552 2.38e-13 70.9
MS.gene81017.t1 AT5G60100 65.217 46 16 0 238 283 441 486 2.40e-13 70.5
MS.gene81017.t1 AT5G60100 65.217 46 16 0 238 283 441 486 2.40e-13 70.5
MS.gene81017.t1 AT5G60100 65.217 46 16 0 238 283 441 486 2.40e-13 70.5
MS.gene81017.t1 AT5G60100 68.182 44 14 0 238 281 468 511 3.25e-13 70.1

Find 68 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATATTCATTGGGTAATA+GGG 0.232593 3.4:-29962039 None:intergenic
GCACATATTCATTGGGTAAT+AGG 0.254453 3.4:-29962040 None:intergenic
GAACAATAGGTCCCATTTCT+TGG 0.266361 3.4:-29961730 None:intergenic
GGACAATGAACCCCATTTCT+TGG 0.284413 3.4:-29961811 None:intergenic
TTGTACTCCACCTGCATCTT+TGG 0.287125 3.4:+29961648 MS.gene81017:CDS
ACTCCCGATGACAAATAATA+TGG 0.320119 3.4:-29961874 None:intergenic
GGTTGTAAGCACATATTCAT+TGG 0.352891 3.4:-29962048 None:intergenic
AGTCTTATACATCCAAGAAA+TGG 0.355720 3.4:+29961718 MS.gene81017:CDS
CAACCAGGTCAGATTCCTTC+AGG 0.363330 3.4:+29962066 MS.gene81017:CDS
CAAAATTAAGTAAAGTTGAT+AGG 0.368608 3.4:+29962124 MS.gene81017:CDS
TGTCTGATGCATCCAAGAAA+TGG 0.381220 3.4:+29961799 MS.gene81017:CDS
GTCTGATGCATCCAAGAAAT+GGG 0.388638 3.4:+29961800 MS.gene81017:CDS
GGTGTTAATGTGGATCTTAA+TGG 0.393979 3.4:+29962282 MS.gene81017:CDS
CAGCATGAAACAATCCTTCA+AGG 0.394565 3.4:-29961459 None:intergenic
CTTATCGAGCTCAGAACAAT+AGG 0.397465 3.4:-29961743 None:intergenic
TATCCATATTATTTGTCATC+GGG 0.404835 3.4:+29961871 MS.gene81017:CDS
ACCAGGTATACTATTGCTGC+TGG 0.455033 3.4:-29961605 None:intergenic
GTTGTAAGCACATATTCATT+GGG 0.463517 3.4:-29962047 None:intergenic
AACTTCCATCCTCACATGTA+AGG 0.465807 3.4:-29961491 None:intergenic
CATGCTAAAAGAAACCTTGA+AGG 0.470407 3.4:+29961445 MS.gene81017:CDS
TGATACATTTGCGCCGATGA+AGG 0.476546 3.4:-29961913 None:intergenic
CCCAGCAGCAATAGTATACC+TGG 0.477013 3.4:+29961604 MS.gene81017:CDS
ATTGAATATGATGAAAATGA+AGG 0.483324 3.4:+29962318 MS.gene81017:CDS
GTCTTATACATCCAAGAAAT+GGG 0.483992 3.4:+29961719 MS.gene81017:CDS
ATGTTAGATCTTTCACGCTC+AGG 0.497470 3.4:-29961832 None:intergenic
CTCTCAATAGATAAAGAACC+AGG 0.507067 3.4:-29961622 None:intergenic
GGTCAGTCATGGCCATCCTT+CGG 0.511134 3.4:+29962087 MS.gene81017:CDS
TGTGAGGCTTCCAAAGATGC+AGG 0.515634 3.4:-29961658 None:intergenic
TCATGTTATGATGCCTTCAT+CGG 0.517737 3.4:+29961900 MS.gene81017:CDS
AGAAAATTAGGTATGTCAAT+CGG 0.521151 3.4:+29962202 MS.gene81017:CDS
CAGCATGATTCTGCAGGTCG+TGG 0.521808 3.4:-29961945 None:intergenic
GACATCATTCCGGTGGTATG+AGG 0.533530 3.4:-29962012 None:intergenic
CATGAAGCATGTGCTCAACA+AGG 0.536975 3.4:+29961517 MS.gene81017:CDS
TTATCCATATTATTTGTCAT+CGG 0.538736 3.4:+29961870 MS.gene81017:CDS
ATAGGGGTAGGACATCATTC+CGG 0.544576 3.4:-29962022 None:intergenic
CCAGGTATACTATTGCTGCT+GGG 0.549234 3.4:-29961604 None:intergenic
ATGAATATGTGCTTACAACC+AGG 0.553880 3.4:+29962051 MS.gene81017:CDS
TTGAATATGATGAAAATGAA+GGG 0.559631 3.4:+29962319 MS.gene81017:CDS
ATGTACCAGCATGATTCTGC+AGG 0.560138 3.4:-29961951 None:intergenic
CACGACCTGCAGAATCATGC+TGG 0.560739 3.4:+29961946 MS.gene81017:CDS
TCGGATGAACTTCCGAAGGA+TGG 0.565075 3.4:-29962099 None:intergenic
TGAAAGACGGCCTCGTGTGA+GGG 0.569464 3.4:+29962236 MS.gene81017:CDS
TGCTTCGGATGAACTTCCGA+AGG 0.577350 3.4:-29962103 None:intergenic
ATTCATTGGGTAATAGGGGT+AGG 0.578636 3.4:-29962034 None:intergenic
AGATTCCTTCAGGTCAGTCA+TGG 0.579147 3.4:+29962076 MS.gene81017:CDS
CAGTTTGTCAGTAAGATGAA+TGG 0.579522 3.4:+29962261 MS.gene81017:CDS
CAGGTATACTATTGCTGCTG+GGG 0.581806 3.4:-29961603 None:intergenic
GGAAACAACTTGCTGAAAGA+CGG 0.584344 3.4:+29962223 MS.gene81017:CDS
CTGAAAGACGGCCTCGTGTG+AGG 0.586756 3.4:+29962235 MS.gene81017:CDS
GAAAGACGGCCTCGTGTGAG+GGG 0.590286 3.4:+29962237 MS.gene81017:CDS
TGCCGCCTTACATGTGAGGA+TGG 0.593042 3.4:+29961486 MS.gene81017:CDS
GATGGCCATGACTGACCTGA+AGG 0.597115 3.4:-29962081 None:intergenic
ACATATTCATTGGGTAATAG+GGG 0.603859 3.4:-29962038 None:intergenic
ATGAAGCATGTGCTCAACAA+GGG 0.604325 3.4:+29961518 MS.gene81017:CDS
TCATCATATTCAATGGAAGG+AGG 0.605016 3.4:-29962309 None:intergenic
TAAGATGAATGGTGTTAATG+TGG 0.608624 3.4:+29962272 MS.gene81017:CDS
CAAGGCGGTCCTCATACCAC+CGG 0.623009 3.4:+29962003 MS.gene81017:CDS
CACTACAATCATGTTCCACA+AGG 0.630620 3.4:+29961985 MS.gene81017:CDS
GTGGTATGAGGACCGCCTTG+TGG 0.638168 3.4:-29962000 None:intergenic
TGACCTGAAGGAATCTGACC+TGG 0.642802 3.4:-29962069 None:intergenic
GAGGCTTCCAAAGATGCAGG+TGG 0.655405 3.4:-29961655 None:intergenic
TTCCATCCTCACATGTAAGG+CGG 0.658020 3.4:-29961488 None:intergenic
ACAAACTGTCCCCTCACACG+AGG 0.662346 3.4:-29962246 None:intergenic
TGAAGCATGTGCTCAACAAG+GGG 0.671878 3.4:+29961519 MS.gene81017:CDS
TACAATCATGTTCCACAAGG+CGG 0.678482 3.4:+29961988 MS.gene81017:CDS
GGGGTAGGACATCATTCCGG+TGG 0.679136 3.4:-29962019 None:intergenic
TCTGATGCATCCAAGAAATG+GGG 0.684504 3.4:+29961801 MS.gene81017:CDS
ACAATGCCGCCTTACATGTG+AGG 0.719839 3.4:+29961482 MS.gene81017:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAATATGATGAAAATGA+AGG + chr3.4:29962318-29962337 MS.gene81017:CDS 20.0%
!! TTATCCATATTATTTGTCAT+CGG + chr3.4:29961870-29961889 MS.gene81017:CDS 20.0%
!! TTGAATATGATGAAAATGAA+GGG + chr3.4:29962319-29962338 MS.gene81017:CDS 20.0%
!!! AACTTTACTTAATTTTGCTT+CGG - chr3.4:29962121-29962140 None:intergenic 20.0%
!!! CAAAATTAAGTAAAGTTGAT+AGG + chr3.4:29962124-29962143 MS.gene81017:CDS 20.0%
!!! TTCATTTTCATCATATTCAA+TGG - chr3.4:29962319-29962338 None:intergenic 20.0%
! AGAAAATTAGGTATGTCAAT+CGG + chr3.4:29962202-29962221 MS.gene81017:CDS 25.0%
! TATCCATATTATTTGTCATC+GGG + chr3.4:29961871-29961890 MS.gene81017:CDS 25.0%
!! TTTTCATCATATTCAATGGA+AGG - chr3.4:29962315-29962334 None:intergenic 25.0%
!!! GCTGTTTTGATAAGAAAATT+AGG + chr3.4:29962190-29962209 MS.gene81017:CDS 25.0%
ACATATTCATTGGGTAATAG+GGG - chr3.4:29962041-29962060 None:intergenic 30.0%
CACATATTCATTGGGTAATA+GGG - chr3.4:29962042-29962061 None:intergenic 30.0%
GTCTTATACATCCAAGAAAT+GGG + chr3.4:29961719-29961738 MS.gene81017:CDS 30.0%
GTTGTAAGCACATATTCATT+GGG - chr3.4:29962050-29962069 None:intergenic 30.0%
! AGTCTTATACATCCAAGAAA+TGG + chr3.4:29961718-29961737 MS.gene81017:CDS 30.0%
! GCAAATTTTCTTCTTCGTAA+CGG - chr3.4:29961690-29961709 None:intergenic 30.0%
! TAAGATGAATGGTGTTAATG+TGG + chr3.4:29962272-29962291 MS.gene81017:CDS 30.0%
ACTCCCGATGACAAATAATA+TGG - chr3.4:29961877-29961896 None:intergenic 35.0%
ATGAATATGTGCTTACAACC+AGG + chr3.4:29962051-29962070 MS.gene81017:CDS 35.0%
CAGTTTGTCAGTAAGATGAA+TGG + chr3.4:29962261-29962280 MS.gene81017:CDS 35.0%
CATGCTAAAAGAAACCTTGA+AGG + chr3.4:29961445-29961464 MS.gene81017:CDS 35.0%
CTCTCAATAGATAAAGAACC+AGG - chr3.4:29961625-29961644 None:intergenic 35.0%
GATCTCGAAGTATGCAAAAA+TGG + chr3.4:29961547-29961566 MS.gene81017:CDS 35.0%
GCACATATTCATTGGGTAAT+AGG - chr3.4:29962043-29962062 None:intergenic 35.0%
GGTGTTAATGTGGATCTTAA+TGG + chr3.4:29962282-29962301 MS.gene81017:CDS 35.0%
GGTTGTAAGCACATATTCAT+TGG - chr3.4:29962051-29962070 None:intergenic 35.0%
TCATCATATTCAATGGAAGG+AGG - chr3.4:29962312-29962331 None:intergenic 35.0%
TCATGTTATGATGCCTTCAT+CGG + chr3.4:29961900-29961919 MS.gene81017:CDS 35.0%
AACTTCCATCCTCACATGTA+AGG - chr3.4:29961494-29961513 None:intergenic 40.0%
ATGAAGCATGTGCTCAACAA+GGG + chr3.4:29961518-29961537 MS.gene81017:CDS 40.0%
ATGTTAGATCTTTCACGCTC+AGG - chr3.4:29961835-29961854 None:intergenic 40.0%
ATTCATTGGGTAATAGGGGT+AGG - chr3.4:29962037-29962056 None:intergenic 40.0%
CACTACAATCATGTTCCACA+AGG + chr3.4:29961985-29962004 MS.gene81017:CDS 40.0%
CAGCATGAAACAATCCTTCA+AGG - chr3.4:29961462-29961481 None:intergenic 40.0%
CTTATCGAGCTCAGAACAAT+AGG - chr3.4:29961746-29961765 None:intergenic 40.0%
GAACAATAGGTCCCATTTCT+TGG - chr3.4:29961733-29961752 None:intergenic 40.0%
GTCTGATGCATCCAAGAAAT+GGG + chr3.4:29961800-29961819 MS.gene81017:CDS 40.0%
TACAATCATGTTCCACAAGG+CGG + chr3.4:29961988-29962007 MS.gene81017:CDS 40.0%
TCTGATGCATCCAAGAAATG+GGG + chr3.4:29961801-29961820 MS.gene81017:CDS 40.0%
TGTCTGATGCATCCAAGAAA+TGG + chr3.4:29961799-29961818 MS.gene81017:CDS 40.0%
! TTCGTAACGGTTTTTCTGTG+AGG - chr3.4:29961677-29961696 None:intergenic 40.0%
! TTGTGATGCAAATTTTGCGC+AGG - chr3.4:29961778-29961797 None:intergenic 40.0%
!! GGAAACAACTTGCTGAAAGA+CGG + chr3.4:29962223-29962242 MS.gene81017:CDS 40.0%
ACCAGGTATACTATTGCTGC+TGG - chr3.4:29961608-29961627 None:intergenic 45.0%
AGATTCCTTCAGGTCAGTCA+TGG + chr3.4:29962076-29962095 MS.gene81017:CDS 45.0%
ATAGGGGTAGGACATCATTC+CGG - chr3.4:29962025-29962044 None:intergenic 45.0%
ATGTACCAGCATGATTCTGC+AGG - chr3.4:29961954-29961973 None:intergenic 45.0%
CAGGTATACTATTGCTGCTG+GGG - chr3.4:29961606-29961625 None:intergenic 45.0%
CATGAAGCATGTGCTCAACA+AGG + chr3.4:29961517-29961536 MS.gene81017:CDS 45.0%
CCAGGTATACTATTGCTGCT+GGG - chr3.4:29961607-29961626 None:intergenic 45.0%
GGACAATGAACCCCATTTCT+TGG - chr3.4:29961814-29961833 None:intergenic 45.0%
TGAAGCATGTGCTCAACAAG+GGG + chr3.4:29961519-29961538 MS.gene81017:CDS 45.0%
TTCCATCCTCACATGTAAGG+CGG - chr3.4:29961491-29961510 None:intergenic 45.0%
TTGTACTCCACCTGCATCTT+TGG + chr3.4:29961648-29961667 MS.gene81017:CDS 45.0%
! TGATACATTTGCGCCGATGA+AGG - chr3.4:29961916-29961935 None:intergenic 45.0%
ACAATGCCGCCTTACATGTG+AGG + chr3.4:29961482-29961501 MS.gene81017:CDS 50.0%
CAACCAGGTCAGATTCCTTC+AGG + chr3.4:29962066-29962085 MS.gene81017:CDS 50.0%
CCCAGCAGCAATAGTATACC+TGG + chr3.4:29961604-29961623 MS.gene81017:CDS 50.0%
GACATCATTCCGGTGGTATG+AGG - chr3.4:29962015-29962034 None:intergenic 50.0%
TCGGATGAACTTCCGAAGGA+TGG - chr3.4:29962102-29962121 None:intergenic 50.0%
TGACCTGAAGGAATCTGACC+TGG - chr3.4:29962072-29962091 None:intergenic 50.0%
TGCTTCGGATGAACTTCCGA+AGG - chr3.4:29962106-29962125 None:intergenic 50.0%
TGTGAGGCTTCCAAAGATGC+AGG - chr3.4:29961661-29961680 None:intergenic 50.0%
ACAAACTGTCCCCTCACACG+AGG - chr3.4:29962249-29962268 None:intergenic 55.0%
CACGACCTGCAGAATCATGC+TGG + chr3.4:29961946-29961965 MS.gene81017:CDS 55.0%
CAGCATGATTCTGCAGGTCG+TGG - chr3.4:29961948-29961967 None:intergenic 55.0%
GAGGCTTCCAAAGATGCAGG+TGG - chr3.4:29961658-29961677 None:intergenic 55.0%
GATGGCCATGACTGACCTGA+AGG - chr3.4:29962084-29962103 None:intergenic 55.0%
GGTCAGTCATGGCCATCCTT+CGG + chr3.4:29962087-29962106 MS.gene81017:CDS 55.0%
TGAAAGACGGCCTCGTGTGA+GGG + chr3.4:29962236-29962255 MS.gene81017:CDS 55.0%
TGCCGCCTTACATGTGAGGA+TGG + chr3.4:29961486-29961505 MS.gene81017:CDS 55.0%
CAAGGCGGTCCTCATACCAC+CGG + chr3.4:29962003-29962022 MS.gene81017:CDS 60.0%
CTGAAAGACGGCCTCGTGTG+AGG + chr3.4:29962235-29962254 MS.gene81017:CDS 60.0%
GAAAGACGGCCTCGTGTGAG+GGG + chr3.4:29962237-29962256 MS.gene81017:CDS 60.0%
GGGGTAGGACATCATTCCGG+TGG - chr3.4:29962022-29962041 None:intergenic 60.0%
GTGGTATGAGGACCGCCTTG+TGG - chr3.4:29962003-29962022 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 29961433 29962347 29961433 ID=MS.gene81017
chr3.4 mRNA 29961433 29962347 29961433 ID=MS.gene81017.t1;Parent=MS.gene81017
chr3.4 exon 29961433 29962347 29961433 ID=MS.gene81017.t1.exon1;Parent=MS.gene81017.t1
chr3.4 CDS 29961433 29962347 29961433 ID=cds.MS.gene81017.t1;Parent=MS.gene81017.t1
Gene Sequence

>MS.gene81017

ATGTTAAAGCATCATGCTAAAAGAAACCTTGAAGGATTGTTTCATGCTGACAATGCCGCCTTACATGTGAGGATGGAAGTTTTGCATGAAGCATGTGCTCAACAAGGGGTTAGTGATCTCGAAGTATGCAAAAATGGAGAAACTTGCGATAGTCAATCGCAAGATGACTTCCCCAGCAGCAATAGTATACCTGGTTCTTTATCTATTGAGAGATCTTGTACTCCACCTGCATCTTTGGAAGCCTCACAGAAAAACCGTTACGAAGAAGAAAATTTGCATCACAACAGTCTTATACATCCAAGAAATGGGACCTATTGTTCTGAGCTCGATAAGTCTAACATGCCTGCGCAAAATTTGCATCACAACTGTCTGATGCATCCAAGAAATGGGGTTCATTGTCCTGAGCGTGAAAGATCTAACATGACTGCACAACATGCTTATCCATATTATTTGTCATCGGGAGTTAATCATGTTATGATGCCTTCATCGGCGCAAATGTATCAAAAGAATGTCCACGACCTGCAGAATCATGCTGGTACATCTATGATTGCTCACTACAATCATGTTCCACAAGGCGGTCCTCATACCACCGGAATGATGTCCTACCCCTATTACCCAATGAATATGTGCTTACAACCAGGTCAGATTCCTTCAGGTCAGTCATGGCCATCCTTCGGAAGTTCATCCGAAGCAAAATTAAGTAAAGTTGATAGGAGAGAAGCAGCATTGATTAAATTCAGACAGAAAAAGAAAGAGCGCTGTTTTGATAAGAAAATTAGGTATGTCAATCGGAAACAACTTGCTGAAAGACGGCCTCGTGTGAGGGGACAGTTTGTCAGTAAGATGAATGGTGTTAATGTGGATCTTAATGGACAGCCTCCTTCCATTGAATATGATGAAAATGAAGGGAGCTAG

Protein sequence

>MS.gene81017.t1

MLKHHAKRNLEGLFHADNAALHVRMEVLHEACAQQGVSDLEVCKNGETCDSQSQDDFPSSNSIPGSLSIERSCTPPASLEASQKNRYEEENLHHNSLIHPRNGTYCSELDKSNMPAQNLHHNCLMHPRNGVHCPERERSNMTAQHAYPYYLSSGVNHVMMPSSAQMYQKNVHDLQNHAGTSMIAHYNHVPQGGPHTTGMMSYPYYPMNMCLQPGQIPSGQSWPSFGSSSEAKLSKVDRREAALIKFRQKKKERCFDKKIRYVNRKQLAERRPRVRGQFVSKMNGVNVDLNGQPPSIEYDENEGS