Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81768.t1 | PNY07356.1 | 97.7 | 351 | 8 | 0 | 91 | 441 | 87 | 437 | 7.30E-185 | 656.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81768.t1 | Q9FG38 | 81.7 | 350 | 62 | 1 | 93 | 440 | 52 | 401 | 6.8e-155 | 548.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81768.t1 | A0A2K3NWE0 | 97.7 | 351 | 8 | 0 | 91 | 441 | 87 | 437 | 5.3e-185 | 656.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050439 | MS.gene81768 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene81768 | 0.801335 | 9.45E-49 | -1.69E-46 |
| MS.gene056638 | MS.gene81768 | 0.814571 | 1.44E-51 | -1.69E-46 |
| MS.gene057360 | MS.gene81768 | 0.828998 | 6.58E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81768.t1 | MTR_1g059650 | 97.721 | 351 | 6 | 1 | 93 | 441 | 54 | 404 | 0.0 | 697 |
| MS.gene81768.t1 | MTR_5g073280 | 25.320 | 391 | 236 | 9 | 93 | 432 | 169 | 554 | 4.75e-22 | 99.0 |
| MS.gene81768.t1 | MTR_5g083370 | 25.714 | 385 | 236 | 8 | 105 | 440 | 130 | 513 | 7.13e-18 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81768.t1 | AT5G06140 | 81.714 | 350 | 62 | 1 | 93 | 440 | 52 | 401 | 0.0 | 585 |
| MS.gene81768.t1 | AT5G07120 | 25.195 | 385 | 237 | 7 | 93 | 428 | 175 | 557 | 4.82e-24 | 105 |
| MS.gene81768.t1 | AT5G07120 | 25.195 | 385 | 237 | 7 | 93 | 428 | 175 | 557 | 4.82e-24 | 105 |
| MS.gene81768.t1 | AT5G58440 | 24.552 | 391 | 235 | 9 | 93 | 428 | 185 | 570 | 9.03e-24 | 104 |
| MS.gene81768.t1 | AT5G07120 | 31.401 | 207 | 114 | 3 | 93 | 271 | 175 | 381 | 6.08e-23 | 102 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACAATCACTGTCAGATTT+TGG | 0.169494 | 1.3:+43299594 | MS.gene81768:CDS |
| GGAAAAGCTGTGAAACTTCT+AGG | 0.218254 | 1.3:+43299615 | MS.gene81768:CDS |
| GATTTCATTCAGGGTACTAT+TGG | 0.274770 | 1.3:+43285967 | MS.gene81768:CDS |
| AAATTAATGAATGAAGAAAT+AGG | 0.281475 | 1.3:+43300708 | MS.gene81768:CDS |
| GAGGAACATGCTTCTAGTTT+AGG | 0.311716 | 1.3:-43300840 | None:intergenic |
| CCTGACCTTTGGCAAATTCA+TGG | 0.318819 | 1.3:-43300773 | None:intergenic |
| CCGTGCTGTACAATCTATTA+AGG | 0.320249 | 1.3:+43299893 | MS.gene81768:CDS |
| CTTCTCGGGTCCCTGGTATT+CGG | 0.328449 | 1.3:-43295604 | None:intergenic |
| GGAACAGAAAACCTTAGATA+TGG | 0.331291 | 1.3:+43300740 | MS.gene81768:CDS |
| TTCTCGGGTCCCTGGTATTC+GGG | 0.332236 | 1.3:-43295603 | None:intergenic |
| TGCTAGGCGTGCCTGACCTT+TGG | 0.367036 | 1.3:-43300784 | None:intergenic |
| CCGAGAGGGCCAATGCCTTC+AGG | 0.367909 | 1.3:+43300156 | MS.gene81768:CDS |
| AGGTATTCAGGATTTCATTC+AGG | 0.370929 | 1.3:+43285957 | MS.gene81768:intron |
| GGGAAAGCATTCTCTGAACT+TGG | 0.378095 | 1.3:+43299660 | MS.gene81768:CDS |
| GACGTTACAGTGACTTTGTT+TGG | 0.388154 | 1.3:+43295637 | MS.gene81768:CDS |
| GAAAGCAATCCCCATATCTA+AGG | 0.390368 | 1.3:-43300751 | None:intergenic |
| TGCAGACAATGGAGAGGTTA+AGG | 0.409471 | 1.3:+43298854 | MS.gene81768:intron |
| CCATGAATTTGCCAAAGGTC+AGG | 0.409942 | 1.3:+43300773 | MS.gene81768:CDS |
| ACTTCTAATAATGTAATACT+TGG | 0.421130 | 1.3:+43285994 | MS.gene81768:CDS |
| CTTCAGGAGGCAATGTGAAC+TGG | 0.425411 | 1.3:+43300172 | MS.gene81768:CDS |
| AACTCACAGTTTCAAAGAAA+AGG | 0.427424 | 1.3:-43286598 | None:intergenic |
| TTCCCGCGAGGGTAAAGAAT+TGG | 0.431003 | 1.3:+43286559 | MS.gene81768:CDS |
| TGAGATGAGGCGACAGGCAT+TGG | 0.431023 | 1.3:+43298231 | MS.gene81768:CDS |
| CGATGTTGATGCTGAACAAA+AGG | 0.438424 | 1.3:+43286029 | MS.gene81768:CDS |
| GAACAGAAAACCTTAGATAT+GGG | 0.442187 | 1.3:+43300741 | MS.gene81768:CDS |
| TGCCTTTGAACTTCTCGAAA+AGG | 0.443527 | 1.3:-43295671 | None:intergenic |
| GGTATTCAGGATTTCATTCA+GGG | 0.447155 | 1.3:+43285958 | MS.gene81768:intron |
| TCAGCATCTGATTCTTCCAC+TGG | 0.455392 | 1.3:-43299313 | None:intergenic |
| CTTCTAATAATGTAATACTT+GGG | 0.475809 | 1.3:+43285995 | MS.gene81768:CDS |
| ACGTCGGATGACAATCTTCT+CGG | 0.479062 | 1.3:-43295619 | None:intergenic |
| AAAGTCAGCGATGTTGTGCT+TGG | 0.479697 | 1.3:+43299280 | MS.gene81768:CDS |
| AATCGTATAATTCAAACAAA+AGG | 0.490807 | 1.3:-43286640 | None:intergenic |
| AGGTTAAGGTCCCACGAGAC+TGG | 0.491430 | 1.3:+43298868 | MS.gene81768:CDS |
| AATTAATGAATGAAGAAATA+GGG | 0.493810 | 1.3:+43300709 | MS.gene81768:CDS |
| CAATAAACTCAGCACTGAAA+CGG | 0.502998 | 1.3:-43298210 | None:intergenic |
| CCTGAAGGCATTGGCCCTCT+CGG | 0.506187 | 1.3:-43300156 | None:intergenic |
| GCTTCTGAAGGAAATGCACT+TGG | 0.512853 | 1.3:+43299639 | MS.gene81768:CDS |
| AGCCAATTCTTTACCCTCGC+GGG | 0.514565 | 1.3:-43286561 | None:intergenic |
| ACATTGCCTCCTGAAGGCAT+TGG | 0.515353 | 1.3:-43300165 | None:intergenic |
| AAGTCAGCGATGTTGTGCTT+GGG | 0.516187 | 1.3:+43299281 | MS.gene81768:CDS |
| TGACAATCTTCTCGGGTCCC+TGG | 0.519332 | 1.3:-43295611 | None:intergenic |
| TTCTAATAATGTAATACTTG+GGG | 0.524156 | 1.3:+43285996 | MS.gene81768:CDS |
| GTTTATTGAGATGAGGCGAC+AGG | 0.531375 | 1.3:+43298225 | MS.gene81768:CDS |
| CATCTGCAATACCGTTTGCT+AGG | 0.531559 | 1.3:-43300800 | None:intergenic |
| GGAAAGCATTCTCTGAACTT+GGG | 0.540094 | 1.3:+43299661 | MS.gene81768:CDS |
| CTGATAAACTCGCTATGAAG+AGG | 0.557238 | 1.3:-43300859 | None:intergenic |
| GGTCAGGCACGCCTAGCAAA+CGG | 0.559431 | 1.3:+43300789 | MS.gene81768:CDS |
| AAAATCTGCATCAAATCAGA+TGG | 0.560547 | 1.3:-43298904 | None:intergenic |
| GCACGGACATAGTCTTTCAA+AGG | 0.563184 | 1.3:-43299876 | None:intergenic |
| CGTCGGATGACAATCTTCTC+GGG | 0.568219 | 1.3:-43295618 | None:intergenic |
| GAGGGTAAAGAATTGGCTGA+TGG | 0.569473 | 1.3:+43286566 | MS.gene81768:CDS |
| AGGGTAAAGAATTGGCTGAT+GGG | 0.570266 | 1.3:+43286567 | MS.gene81768:CDS |
| GGCTGAAACAATGAAGCTGA+AGG | 0.574052 | 1.3:+43300193 | MS.gene81768:CDS |
| GTGCTGAGTTTATTGAGATG+AGG | 0.574260 | 1.3:+43298218 | MS.gene81768:CDS |
| ATGCATATCGTCTTGTGAAG+AGG | 0.578268 | 1.3:+43299398 | MS.gene81768:CDS |
| CAGCGATGTTGTGCTTGGGA+AGG | 0.585198 | 1.3:+43299285 | MS.gene81768:CDS |
| CTCCAGGTAACTTCCCGCGA+GGG | 0.586190 | 1.3:+43286548 | MS.gene81768:intron |
| CTTCCAGAGAAAAGTGCTGT+AGG | 0.587040 | 1.3:+43295708 | MS.gene81768:CDS |
| CAGTTCACATTGCCTCCTGA+AGG | 0.587473 | 1.3:-43300171 | None:intergenic |
| GCTTTCCATGAATTTGCCAA+AGG | 0.588195 | 1.3:+43300768 | MS.gene81768:CDS |
| CACATGCAGAGTGCAATCAC+AGG | 0.593272 | 1.3:+43286686 | MS.gene81768:CDS |
| TTCGACAAAGTGAGGATCTG+AGG | 0.602775 | 1.3:+43298290 | MS.gene81768:CDS |
| CAGCCAATTCTTTACCCTCG+CGG | 0.604562 | 1.3:-43286562 | None:intergenic |
| TCTAATAATGTAATACTTGG+GGG | 0.607884 | 1.3:+43285997 | MS.gene81768:CDS |
| TCTTGAAGATACCAGTCTCG+TGG | 0.615242 | 1.3:-43298879 | None:intergenic |
| AAACAAAGTCACTGTAACGT+CGG | 0.615739 | 1.3:-43295635 | None:intergenic |
| CTTCTGAAGGAAATGCACTT+GGG | 0.616924 | 1.3:+43299640 | MS.gene81768:CDS |
| ACAGGCAACAATAGCCGAGA+GGG | 0.619770 | 1.3:+43300142 | MS.gene81768:intron |
| AAACTTCTAGGAGCTTCTGA+AGG | 0.620311 | 1.3:+43299627 | MS.gene81768:CDS |
| AACAGAAAACCTTAGATATG+GGG | 0.624575 | 1.3:+43300742 | MS.gene81768:CDS |
| ATCAGTCAAGCTGCAAAACG+AGG | 0.625253 | 1.3:+43299701 | MS.gene81768:CDS |
| AGAGGGCCAATGCCTTCAGG+AGG | 0.633470 | 1.3:+43300159 | MS.gene81768:CDS |
| TCATGAACTTCGACAAAGTG+AGG | 0.634279 | 1.3:+43298282 | MS.gene81768:CDS |
| CAAACGGTATTGCAGATGCA+TGG | 0.635267 | 1.3:+43300805 | MS.gene81768:CDS |
| CTTGAAGATACCAGTCTCGT+GGG | 0.648516 | 1.3:-43298878 | None:intergenic |
| ATACTTTGCAGACAATGGAG+AGG | 0.673981 | 1.3:+43298848 | MS.gene81768:intron |
| CCTTAATAGATTGTACAGCA+CGG | 0.685821 | 1.3:-43299893 | None:intergenic |
| TCTCCAGGTAACTTCCCGCG+AGG | 0.687110 | 1.3:+43286547 | MS.gene81768:intron |
| GCTTGGGAAGGAGAAGCCAG+TGG | 0.688575 | 1.3:+43299297 | MS.gene81768:CDS |
| AGCTGAGCGTGAATATCACG+AGG | 0.701432 | 1.3:+43300551 | MS.gene81768:CDS |
| CGTCTTGTGAAGAGGCACAG+AGG | 0.718716 | 1.3:+43299406 | MS.gene81768:CDS |
| AACAGGCAACAATAGCCGAG+AGG | 0.740560 | 1.3:+43300141 | MS.gene81768:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 43285759 | 43300869 | 43285759 | ID=MS.gene81768 |
| chr1.3 | mRNA | 43285759 | 43300869 | 43285759 | ID=MS.gene81768.t1;Parent=MS.gene81768 |
| chr1.3 | exon | 43285759 | 43285788 | 43285759 | ID=MS.gene81768.t1.exon1;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43285759 | 43285788 | 43285759 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43285967 | 43286050 | 43285967 | ID=MS.gene81768.t1.exon2;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43285967 | 43286050 | 43285967 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43286554 | 43286715 | 43286554 | ID=MS.gene81768.t1.exon3;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43286554 | 43286715 | 43286554 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43295594 | 43295729 | 43295594 | ID=MS.gene81768.t1.exon4;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43295594 | 43295729 | 43295594 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43298203 | 43298336 | 43298203 | ID=MS.gene81768.t1.exon5;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43298203 | 43298336 | 43298203 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43298859 | 43298930 | 43298859 | ID=MS.gene81768.t1.exon6;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43298859 | 43298930 | 43298859 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43299268 | 43299427 | 43299268 | ID=MS.gene81768.t1.exon7;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43299268 | 43299427 | 43299268 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43299589 | 43299722 | 43299589 | ID=MS.gene81768.t1.exon8;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43299589 | 43299722 | 43299589 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43299846 | 43299914 | 43299846 | ID=MS.gene81768.t1.exon9;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43299846 | 43299914 | 43299846 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43300146 | 43300225 | 43300146 | ID=MS.gene81768.t1.exon10;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43300146 | 43300225 | 43300146 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43300512 | 43300576 | 43300512 | ID=MS.gene81768.t1.exon11;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43300512 | 43300576 | 43300512 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| chr1.3 | exon | 43300670 | 43300869 | 43300670 | ID=MS.gene81768.t1.exon12;Parent=MS.gene81768.t1 |
| chr1.3 | CDS | 43300670 | 43300869 | 43300670 | ID=cds.MS.gene81768.t1;Parent=MS.gene81768.t1 |
| Gene Sequence |
| Protein sequence |
