AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGTCTTCATTTCAAATTT+TGG 0.167361 1.4:-53420543 None:intergenic
TTGATGATCTCTTCTGCTTT+TGG 0.188067 1.4:-53420025 None:intergenic
CAACTACTACAAGAACATTT+TGG 0.233199 1.4:+53420990 MS.gene82532:CDS
TTCACATAAGGTGATGTTCT+TGG 0.257639 1.4:-53421057 None:intergenic
AAGGGTACATTGCATGAATT+TGG 0.274410 1.4:+53420822 MS.gene82532:CDS
CAAATAATCATATAACTAAT+TGG 0.281961 1.4:-53421205 None:intergenic
TTTCTCAACAGCAGCTTTGA+TGG 0.284452 1.4:-53420319 None:intergenic
GCATATGTGGGGTCTAGCTT+TGG 0.306524 1.4:-53420871 None:intergenic
CTAAGGATATAGAGTCATTA+TGG 0.325868 1.4:-53420620 None:intergenic
CAGACATCAGAGAGAAGTTC+CGG 0.336911 1.4:+53420270 MS.gene82532:CDS
ACAGCATTTGGATCTGGATT+TGG 0.338871 1.4:-53420922 None:intergenic
CTTGCATATAGAACAGCATT+TGG 0.340382 1.4:-53420934 None:intergenic
TTGGATCTGGATTTGGTGTT+GGG 0.357857 1.4:-53420915 None:intergenic
TCAGAGAATAATCCTCTTAC+CGG 0.371092 1.4:+53421141 MS.gene82532:CDS
TCTAGCTTTGGGTCCACTGT+GGG 0.394817 1.4:-53420859 None:intergenic
CATATGTGGGGTCTAGCTTT+GGG 0.402844 1.4:-53420870 None:intergenic
GAAGCCACTGTTGCTCTGTT+AGG 0.406342 1.4:+53420678 MS.gene82532:CDS
TCACCTTGATCTCCGGTAAG+AGG 0.408149 1.4:-53421153 None:intergenic
GATATAGAGTCATTATGGTT+TGG 0.411553 1.4:-53420615 None:intergenic
CCACTGTGGGGTATAGCCTC+TGG 0.422051 1.4:-53420846 None:intergenic
TCTAATTTCACCTTGATCTC+CGG 0.439413 1.4:-53421160 None:intergenic
TATAGAACAGCATTTGGATC+TGG 0.452583 1.4:-53420928 None:intergenic
TTTGGATCTGGATTTGGTGT+TGG 0.455653 1.4:-53420916 None:intergenic
AGTAGTAGTTCATTTGTAGC+TGG 0.459722 1.4:-53419991 None:intergenic
ATGGAAACACGGCGGTTTCG+TGG 0.470766 1.4:+53420077 MS.gene82532:CDS
AGACATCAGAGAGAAGTTCC+GGG 0.476089 1.4:+53420271 MS.gene82532:CDS
TAGTTGTTGTCGATTATCAT+TGG 0.485138 1.4:-53420973 None:intergenic
AATCCTCTTACCGGAGATCA+AGG 0.499067 1.4:+53421150 MS.gene82532:CDS
GTCGGTAGGCGATGTAGTAT+CGG 0.505099 1.4:+53420245 MS.gene82532:CDS
GTCTAGCTTTGGGTCCACTG+TGG 0.510662 1.4:-53420860 None:intergenic
TCACCTTGACTATGCAATCA+TGG 0.511245 1.4:-53420114 None:intergenic
TTTAGATAGGCAGCATATGT+GGG 0.512711 1.4:-53420883 None:intergenic
CGCCTACCGACTCTAAAAGC+AGG 0.513021 1.4:-53420233 None:intergenic
CCAAAATTTGAAATGAAGAC+AGG 0.514773 1.4:+53420543 MS.gene82532:CDS
ATTCTTCTACCATTGTTACA+TGG 0.531531 1.4:-53420587 None:intergenic
CGCTAGAGATGGAATTGCCA+TGG 0.534187 1.4:+53420386 MS.gene82532:CDS
GTTGCTCTTTCCGCTAGAGA+TGG 0.543064 1.4:+53420375 MS.gene82532:CDS
AGATCAAGGTGAAATTAGAA+AGG 0.559276 1.4:+53421164 MS.gene82532:CDS
CAGGAGGACACTCAGTGGGA+AGG 0.566579 1.4:+53420803 MS.gene82532:intron
ACTTCAATGAACAATTCTCA+AGG 0.567274 1.4:+53421103 MS.gene82532:CDS
ATACTTGAAGTTTCGCATCC+CGG 0.570050 1.4:-53420289 None:intergenic
CTAGCTTTGGGTCCACTGTG+GGG 0.577225 1.4:-53420858 None:intergenic
AGGAGGACACTCAGTGGGAA+GGG 0.577554 1.4:+53420804 MS.gene82532:intron
TGGAAACACGGCGGTTTCGT+GGG 0.579986 1.4:+53420078 MS.gene82532:CDS
ATTGAACTGTATAACGAGCA+TGG 0.584782 1.4:+53420058 MS.gene82532:CDS
CAAAGAAAGCCATGTAACAA+TGG 0.587531 1.4:+53420578 MS.gene82532:CDS
TTGAAATGAAGACAGGACGA+AGG 0.588626 1.4:+53420550 MS.gene82532:CDS
ATGAAGACAGGACGAAGGGA+TGG 0.591567 1.4:+53420555 MS.gene82532:CDS
TGAAATGAAGACAGGACGAA+GGG 0.593661 1.4:+53420551 MS.gene82532:CDS
TTGAAAACGAGAAAGCACTA+AGG 0.594148 1.4:-53420637 None:intergenic
CTTCCATGATTGCATAGTCA+AGG 0.595237 1.4:+53420111 MS.gene82532:CDS
GTCGGCACATGATACTGTCA+AGG 0.601258 1.4:-53420349 None:intergenic
GTATAACGAGCATGGAAACA+CGG 0.609604 1.4:+53420066 MS.gene82532:CDS
GGTGCATTTGTATAGTTGGG+AGG 0.613238 1.4:+53420519 MS.gene82532:intron
GCCTACCGACTCTAAAAGCA+GGG 0.614896 1.4:-53420232 None:intergenic
CTTCAATGAACAATTCTCAA+GGG 0.615864 1.4:+53421104 MS.gene82532:CDS
GTTCTTCATCCACAGTGAGT+AGG 0.617420 1.4:-53421023 None:intergenic
CAATCAGGAGGACACTCAGT+GGG 0.618173 1.4:+53420799 MS.gene82532:intron
ATTGCATGAATTTGGTCCAG+AGG 0.619021 1.4:+53420830 MS.gene82532:CDS
AGCGGAAAGAGCAACAATGT+CGG 0.649811 1.4:-53420367 None:intergenic
CATGGCAATTCCATCTCTAG+CGG 0.653048 1.4:-53420385 None:intergenic
TTAGATAGGCAGCATATGTG+GGG 0.658332 1.4:-53420882 None:intergenic
AGTACCTAACAGAGCAACAG+TGG 0.665213 1.4:-53420682 None:intergenic
TCAATCAGGAGGACACTCAG+TGG 0.675558 1.4:+53420798 MS.gene82532:intron
TCGGCACATGATACTGTCAA+GGG 0.683470 1.4:-53420348 None:intergenic
ACACAAGGGCCTACTCACTG+TGG 0.693288 1.4:+53421014 MS.gene82532:CDS
CCAGAGGCTATACCCCACAG+TGG 0.772742 1.4:+53420846 MS.gene82532:CDS
TAACGAGCATGGAAACACGG+CGG 0.832552 1.4:+53420069 MS.gene82532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAACATAAACATAATTAAT+CGG + chr1.4:53420765-53420784 MS.gene82532:intron 15.0%
!! TATAGAACATTGTAAATATT+GGG + chr1.4:53420480-53420499 MS.gene82532:intron 15.0%
!! TTATAGAACATTGTAAATAT+TGG + chr1.4:53420479-53420498 MS.gene82532:intron 15.0%
!!! AATTGATAATGATATATATG+TGG + chr1.4:53420184-53420203 MS.gene82532:intron 15.0%
!!! ATTCTAACATTTACTTTTTT+TGG + chr1.4:53420712-53420731 MS.gene82532:intron 15.0%
!!! ATTGATAATGATATATATGT+GGG + chr1.4:53420185-53420204 MS.gene82532:intron 15.0%
ACTTCAATGAACAATTCTCA+AGG + chr1.4:53421103-53421122 MS.gene82532:CDS 30.0%
AGATCAAGGTGAAATTAGAA+AGG + chr1.4:53421164-53421183 MS.gene82532:CDS 30.0%
ATCACCTTATGTGAAAAAGA+TGG + chr1.4:53421065-53421084 MS.gene82532:CDS 30.0%
ATTCTTCTACCATTGTTACA+TGG - chr1.4:53420590-53420609 None:intergenic 30.0%
CTAAGGATATAGAGTCATTA+TGG - chr1.4:53420623-53420642 None:intergenic 30.0%
CTTCAATGAACAATTCTCAA+GGG + chr1.4:53421104-53421123 MS.gene82532:CDS 30.0%
GATATAGAGTCATTATGGTT+TGG - chr1.4:53420618-53420637 None:intergenic 30.0%
! CAACTACTACAAGAACATTT+TGG + chr1.4:53420990-53421009 MS.gene82532:CDS 30.0%
! CCAAAATTTGAAATGAAGAC+AGG + chr1.4:53420543-53420562 MS.gene82532:CDS 30.0%
! CCTGTCTTCATTTCAAATTT+TGG - chr1.4:53420546-53420565 None:intergenic 30.0%
! TAGTTGTTGTCGATTATCAT+TGG - chr1.4:53420976-53420995 None:intergenic 30.0%
! TTGGGTGTGAATTTTTATGT+TGG + chr1.4:53420498-53420517 MS.gene82532:intron 30.0%
!!! AAGAACATTTTGGAACACAA+GGG + chr1.4:53421000-53421019 MS.gene82532:CDS 30.0%
AAAAAGATGGCAGCTGATAA+TGG + chr1.4:53421078-53421097 MS.gene82532:CDS 35.0%
AGTAGTAGTTCATTTGTAGC+TGG - chr1.4:53419994-53420013 None:intergenic 35.0%
ATTGAACTGTATAACGAGCA+TGG + chr1.4:53420058-53420077 MS.gene82532:CDS 35.0%
CAAAGAAAGCCATGTAACAA+TGG + chr1.4:53420578-53420597 MS.gene82532:CDS 35.0%
CTTGCATATAGAACAGCATT+TGG - chr1.4:53420937-53420956 None:intergenic 35.0%
GTTGGTGCATTTGTATAGTT+GGG + chr1.4:53420516-53420535 MS.gene82532:intron 35.0%
TATAGAACAGCATTTGGATC+TGG - chr1.4:53420931-53420950 None:intergenic 35.0%
TCAGAGAATAATCCTCTTAC+CGG + chr1.4:53421141-53421160 MS.gene82532:CDS 35.0%
TCGTTACGTTAATGCCAATT+AGG + chr1.4:53421191-53421210 MS.gene82532:CDS 35.0%
TCTAATTTCACCTTGATCTC+CGG - chr1.4:53421163-53421182 None:intergenic 35.0%
TGTTGGTGCATTTGTATAGT+TGG + chr1.4:53420515-53420534 MS.gene82532:intron 35.0%
TTCACATAAGGTGATGTTCT+TGG - chr1.4:53421060-53421079 None:intergenic 35.0%
TTTAGATAGGCAGCATATGT+GGG - chr1.4:53420886-53420905 None:intergenic 35.0%
! AAGGGTACATTGCATGAATT+TGG + chr1.4:53420822-53420841 MS.gene82532:CDS 35.0%
! TTTTAGATAGGCAGCATATG+TGG - chr1.4:53420887-53420906 None:intergenic 35.0%
!! CTTTCTCGTTTTCAAGCTAT+TGG + chr1.4:53420645-53420664 MS.gene82532:CDS 35.0%
!! TGATGATCTCTTCTGCTTTT+GGG - chr1.4:53420027-53420046 None:intergenic 35.0%
!! TTGAAAACGAGAAAGCACTA+AGG - chr1.4:53420640-53420659 None:intergenic 35.0%
!! TTGATGATCTCTTCTGCTTT+TGG - chr1.4:53420028-53420047 None:intergenic 35.0%
!!! CAAGAACATTTTGGAACACA+AGG + chr1.4:53420999-53421018 MS.gene82532:CDS 35.0%
!!! TGGGCATCTAAGTTTTAGAT+AGG - chr1.4:53420899-53420918 None:intergenic 35.0%
ACAGCATTTGGATCTGGATT+TGG - chr1.4:53420925-53420944 None:intergenic 40.0%
ATACTTGAAGTTTCGCATCC+CGG - chr1.4:53420292-53420311 None:intergenic 40.0%
ATTGCATGAATTTGGTCCAG+AGG + chr1.4:53420830-53420849 MS.gene82532:CDS 40.0%
CTTCCATGATTGCATAGTCA+AGG + chr1.4:53420111-53420130 MS.gene82532:CDS 40.0%
GTATAACGAGCATGGAAACA+CGG + chr1.4:53420066-53420085 MS.gene82532:CDS 40.0%
TCACCTTGACTATGCAATCA+TGG - chr1.4:53420117-53420136 None:intergenic 40.0%
TGAAATGAAGACAGGACGAA+GGG + chr1.4:53420551-53420570 MS.gene82532:CDS 40.0%
TTAGATAGGCAGCATATGTG+GGG - chr1.4:53420885-53420904 None:intergenic 40.0%
TTGAAATGAAGACAGGACGA+AGG + chr1.4:53420550-53420569 MS.gene82532:CDS 40.0%
! CGCAGCAGAGTTATCATTTT+TGG + chr1.4:53420150-53420169 MS.gene82532:intron 40.0%
! GTCACTTTGATTCAATCAGG+AGG + chr1.4:53420787-53420806 MS.gene82532:intron 40.0%
! TCGGTCACTTTGATTCAATC+AGG + chr1.4:53420784-53420803 MS.gene82532:intron 40.0%
! TTTCTCAACAGCAGCTTTGA+TGG - chr1.4:53420322-53420341 None:intergenic 40.0%
!! GCTGCCATCTTTTTCACATA+AGG - chr1.4:53421072-53421091 None:intergenic 40.0%
!! TTGGATCTGGATTTGGTGTT+GGG - chr1.4:53420918-53420937 None:intergenic 40.0%
!! TTTGGATCTGGATTTGGTGT+TGG - chr1.4:53420919-53420938 None:intergenic 40.0%
AATCCTCTTACCGGAGATCA+AGG + chr1.4:53421150-53421169 MS.gene82532:CDS 45.0%
AGACATCAGAGAGAAGTTCC+GGG + chr1.4:53420271-53420290 MS.gene82532:CDS 45.0%
AGCGGAAAGAGCAACAATGT+CGG - chr1.4:53420370-53420389 None:intergenic 45.0%
AGTACCTAACAGAGCAACAG+TGG - chr1.4:53420685-53420704 None:intergenic 45.0%
CAGACATCAGAGAGAAGTTC+CGG + chr1.4:53420270-53420289 MS.gene82532:CDS 45.0%
CATGGCAATTCCATCTCTAG+CGG - chr1.4:53420388-53420407 None:intergenic 45.0%
GGTGCATTTGTATAGTTGGG+AGG + chr1.4:53420519-53420538 MS.gene82532:intron 45.0%
GTGCGACTCTTAACATACCA+TGG - chr1.4:53420406-53420425 None:intergenic 45.0%
GTTCTTCATCCACAGTGAGT+AGG - chr1.4:53421026-53421045 None:intergenic 45.0%
TCGGCACATGATACTGTCAA+GGG - chr1.4:53420351-53420370 None:intergenic 45.0%
!! CATATGTGGGGTCTAGCTTT+GGG - chr1.4:53420873-53420892 None:intergenic 45.0%
!! TTCTGCTTTTGGGCAGCTTT+TGG - chr1.4:53420017-53420036 None:intergenic 45.0%
ATGAAGACAGGACGAAGGGA+TGG + chr1.4:53420555-53420574 MS.gene82532:CDS 50.0%
CAATCAGGAGGACACTCAGT+GGG + chr1.4:53420799-53420818 MS.gene82532:intron 50.0%
CGCTAGAGATGGAATTGCCA+TGG + chr1.4:53420386-53420405 MS.gene82532:CDS 50.0%
GAAGCCACTGTTGCTCTGTT+AGG + chr1.4:53420678-53420697 MS.gene82532:CDS 50.0%
GTCGGCACATGATACTGTCA+AGG - chr1.4:53420352-53420371 None:intergenic 50.0%
GTCGGTAGGCGATGTAGTAT+CGG + chr1.4:53420245-53420264 MS.gene82532:CDS 50.0%
GTTGCTCTTTCCGCTAGAGA+TGG + chr1.4:53420375-53420394 MS.gene82532:CDS 50.0%
TAACGAGCATGGAAACACGG+CGG + chr1.4:53420069-53420088 MS.gene82532:CDS 50.0%
TCAATCAGGAGGACACTCAG+TGG + chr1.4:53420798-53420817 MS.gene82532:intron 50.0%
TCACCTTGATCTCCGGTAAG+AGG - chr1.4:53421156-53421175 None:intergenic 50.0%
! GCATATGTGGGGTCTAGCTT+TGG - chr1.4:53420874-53420893 None:intergenic 50.0%
!! GCCTACCGACTCTAAAAGCA+GGG - chr1.4:53420235-53420254 None:intergenic 50.0%
!! TCTAGCTTTGGGTCCACTGT+GGG - chr1.4:53420862-53420881 None:intergenic 50.0%
!!! CGCATCCCTGCTTTTAGAGT+CGG + chr1.4:53420227-53420246 MS.gene82532:CDS 50.0%
!!! TCCCTGCTTTTAGAGTCGGT+AGG + chr1.4:53420231-53420250 MS.gene82532:CDS 50.0%
ACACAAGGGCCTACTCACTG+TGG + chr1.4:53421014-53421033 MS.gene82532:CDS 55.0%
AGGAGGACACTCAGTGGGAA+GGG + chr1.4:53420804-53420823 MS.gene82532:intron 55.0%
ATGGAAACACGGCGGTTTCG+TGG + chr1.4:53420077-53420096 MS.gene82532:CDS 55.0%
TGGAAACACGGCGGTTTCGT+GGG + chr1.4:53420078-53420097 MS.gene82532:CDS 55.0%
!! CGCCTACCGACTCTAAAAGC+AGG - chr1.4:53420236-53420255 None:intergenic 55.0%
!! CTAGCTTTGGGTCCACTGTG+GGG - chr1.4:53420861-53420880 None:intergenic 55.0%
!! GTCTAGCTTTGGGTCCACTG+TGG - chr1.4:53420863-53420882 None:intergenic 55.0%
CAGGAGGACACTCAGTGGGA+AGG + chr1.4:53420803-53420822 MS.gene82532:intron 60.0%
CCACTGTGGGGTATAGCCTC+TGG - chr1.4:53420849-53420868 None:intergenic 60.0%
CCAGAGGCTATACCCCACAG+TGG + chr1.4:53420846-53420865 MS.gene82532:CDS 60.0%
GGATGCGTCGCATGACTGCG+TGG - chr1.4:53420214-53420233 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 53419984 53421212 53419984 ID=MS.gene82532
chr1.4 mRNA 53419984 53421212 53419984 ID=MS.gene82532.t1;Parent=MS.gene82532
chr1.4 exon 53419984 53420132 53419984 ID=MS.gene82532.t1.exon1;Parent=MS.gene82532.t1
chr1.4 CDS 53419984 53420132 53419984 ID=cds.MS.gene82532.t1;Parent=MS.gene82532.t1
chr1.4 exon 53420219 53420407 53420219 ID=MS.gene82532.t1.exon2;Parent=MS.gene82532.t1
chr1.4 CDS 53420219 53420407 53420219 ID=cds.MS.gene82532.t1;Parent=MS.gene82532.t1
chr1.4 exon 53420534 53420699 53420534 ID=MS.gene82532.t1.exon3;Parent=MS.gene82532.t1
chr1.4 CDS 53420534 53420699 53420534 ID=cds.MS.gene82532.t1;Parent=MS.gene82532.t1
chr1.4 exon 53420806 53421212 53420806 ID=MS.gene82532.t1.exon4;Parent=MS.gene82532.t1
chr1.4 CDS 53420806 53421212 53420806 ID=cds.MS.gene82532.t1;Parent=MS.gene82532.t1
Gene Sequence

>MS.gene82532

GAAAAAGCCAGCTACAAATGAACTACTACTCCAAAAGCTGCCCAAAAGCAGAAGAGATCATCAAACAAAAAGTCATTGAACTGTATAACGAGCATGGAAACACGGCGGTTTCGTGGGTTAGAAATCTCTTCCATGATTGCATAGTCAAGGTGAGTGTAAATGTAATCGCAGCAGAGTTATCATTTTTGGATTAAAGAAGCAATTGATAATGATATATATGTGGGTTTCCACGCAGTCATGCGACGCATCCCTGCTTTTAGAGTCGGTAGGCGATGTAGTATCGGAGCAGACATCAGAGAGAAGTTCCGGGATGCGAAACTTCAAGTATGTGAAAACCATCAAAGCTGCTGTTGAGAAAGAATGTCCCTTGACAGTATCATGTGCCGACATTGTTGCTCTTTCCGCTAGAGATGGAATTGCCATGGTATGTTAAGAGTCGCACATTAGAAATCTCACATTAAAAGTGAGACTTAAAATTCTAAAGATTCTTCTATCTTATAGAACATTGTAAATATTGGGTGTGAATTTTTATGTTGGTGCATTTGTATAGTTGGGAGGTCCAAAATTTGAAATGAAGACAGGACGAAGGGATGGCAAAGAAAGCCATGTAACAATGGTAGAAGAATTCATTCCAAACCATAATGACTCTATATCCTTAGTGCTTTCTCGTTTTCAAGCTATTGGTGTTGACGTTGAAGCCACTGTTGCTCTGTTAGGTACTACTGTGCATTCTAACATTTACTTTTTTTGGTCAATACATTCTAACATTGACTTCAAAAGTCAAACATAAACATAATTAATCGGTCACTTTGATTCAATCAGGAGGACACTCAGTGGGAAGGGTACATTGCATGAATTTGGTCCAGAGGCTATACCCCACAGTGGACCCAAAGCTAGACCCCACATATGCTGCCTATCTAAAACTTAGATGCCCAACACCAAATCCAGATCCAAATGCTGTTCTATATGCAAGAAATGATAGAAAAACGCCAATGATAATCGACAACAACTACTACAAGAACATTTTGGAACACAAGGGCCTACTCACTGTGGATGAAGAACTAGCTACTGATCCAAGAACATCACCTTATGTGAAAAAGATGGCAGCTGATAATGGATACTTCAATGAACAATTCTCAAGGGCTGTTCAATTGTTATCAGAGAATAATCCTCTTACCGGAGATCAAGGTGAAATTAGAAAGGATTGTCGTTACGTTAATGCCAATTAG

Protein sequence

>MS.gene82532.t1

EKASYKUTTTPKAAQKQKRSSNKKSLNCITSMETRRFRGLEISSMIAUSSHATHPCFUSRUAMUYRSRHQREVPGCETSSMUKPSKLLLRKNVPUQYHVPTLLLFPLEMELPCWEVQNLKURQDEGMAKKAMUQWUKNSFQTIMTLYPUCFLVFKLLVLTLKPLLLCUEDTQWEGYIAUIWSRGYTPQWTQSUTPHMLPIUNLDAQHQIQIQMLFYMQEMIEKRQUUSTTTTTRTFWNTRAYSLWMKNULLIQEHHLMUKRWQLIMDTSMNNSQGLFNCYQRIILLPEIKVKLERIVVTLMPI