Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83077.t1 | XP_013466570.1 | 98.8 | 165 | 2 | 0 | 1 | 165 | 1 | 165 | 1.40E-91 | 345.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83077.t1 | Q9ZU95 | 69.1 | 162 | 49 | 1 | 1 | 162 | 6 | 166 | 1.3e-63 | 243.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83077.t1 | G8A033 | 98.8 | 165 | 2 | 0 | 1 | 165 | 1 | 165 | 9.8e-92 | 345.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene83077 | 0.831168 | 1.94E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene83077 | 0.806115 | 9.62E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene83077 | 0.808778 | 2.62E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene83077 | 0.811129 | 8.17E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83077.t1 | MTR_1g032290 | 98.788 | 165 | 2 | 0 | 1 | 165 | 1 | 165 | 3.54e-121 | 338 |
| MS.gene83077.t1 | MTR_8g105140 | 81.646 | 158 | 29 | 0 | 1 | 158 | 1 | 158 | 8.33e-97 | 276 |
| MS.gene83077.t1 | MTR_3g065330 | 67.485 | 163 | 50 | 2 | 1 | 161 | 10 | 171 | 5.35e-75 | 223 |
| MS.gene83077.t1 | MTR_5g078020 | 61.963 | 163 | 57 | 3 | 1 | 161 | 10 | 169 | 9.64e-65 | 196 |
| MS.gene83077.t1 | MTR_6g070920 | 59.748 | 159 | 60 | 3 | 1 | 158 | 72 | 227 | 3.44e-63 | 194 |
| MS.gene83077.t1 | MTR_3g065330 | 61.972 | 142 | 51 | 2 | 1 | 140 | 32 | 172 | 1.00e-57 | 178 |
| MS.gene83077.t1 | MTR_6g070920 | 59.420 | 138 | 52 | 3 | 1 | 137 | 72 | 206 | 1.15e-52 | 167 |
| MS.gene83077.t1 | MTR_8g039690 | 46.835 | 158 | 76 | 3 | 1 | 151 | 1 | 157 | 2.04e-41 | 137 |
| MS.gene83077.t1 | MTR_7g084380 | 46.875 | 160 | 74 | 4 | 1 | 150 | 1 | 159 | 5.14e-36 | 124 |
| MS.gene83077.t1 | MTR_6g070920 | 64.045 | 89 | 28 | 3 | 1 | 88 | 72 | 157 | 3.78e-32 | 113 |
| MS.gene83077.t1 | MTR_7g084380 | 46.667 | 135 | 62 | 3 | 26 | 150 | 21 | 155 | 8.76e-30 | 108 |
| MS.gene83077.t1 | MTR_1g078390 | 46.154 | 65 | 33 | 2 | 1 | 64 | 1 | 64 | 8.83e-13 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83077.t1 | AT2G01670 | 69.136 | 162 | 49 | 1 | 1 | 162 | 6 | 166 | 8.58e-82 | 239 |
| MS.gene83077.t1 | AT1G14860 | 71.698 | 159 | 44 | 1 | 1 | 159 | 1 | 158 | 9.97e-82 | 239 |
| MS.gene83077.t1 | AT1G18300 | 54.487 | 156 | 70 | 1 | 1 | 155 | 40 | 195 | 7.36e-62 | 190 |
| MS.gene83077.t1 | AT1G73540 | 54.777 | 157 | 66 | 2 | 1 | 156 | 41 | 193 | 5.27e-60 | 185 |
| MS.gene83077.t1 | AT1G12880 | 46.951 | 164 | 73 | 4 | 1 | 151 | 1 | 163 | 7.58e-38 | 129 |
| MS.gene83077.t1 | AT3G26690 | 45.455 | 165 | 75 | 4 | 1 | 151 | 1 | 164 | 1.52e-37 | 128 |
| MS.gene83077.t1 | AT3G26690 | 45.455 | 165 | 75 | 4 | 1 | 151 | 1 | 164 | 1.52e-37 | 128 |
| MS.gene83077.t1 | AT3G12600 | 48.428 | 159 | 72 | 4 | 1 | 150 | 1 | 158 | 6.44e-35 | 120 |
| MS.gene83077.t1 | AT3G12600 | 48.299 | 147 | 66 | 4 | 13 | 150 | 4 | 149 | 7.21e-31 | 110 |
Find 37 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCTACGTACAAGAATA+TGG | 0.220819 | 1.2:+16783431 | MS.gene83077:CDS |
| CATATTATTGCAGCATGAAT+TGG | 0.282486 | 1.2:+16783312 | MS.gene83077:intron |
| CTTGAAGAAGCAGGAGTTAT+AGG | 0.288124 | 1.2:+16783178 | MS.gene83077:CDS |
| GCAAGACATTGATGGCAATA+TGG | 0.292101 | 1.2:+16782942 | MS.gene83077:CDS |
| CTTTAGATATACTTGTTGAT+AGG | 0.363474 | 1.2:+16783950 | MS.gene83077:CDS |
| GTTTGTTTGGTATCTCGTTC+TGG | 0.365455 | 1.2:+16782507 | MS.gene83077:CDS |
| ATGTCTTGCTTATATCTGTA+TGG | 0.367359 | 1.2:-16782928 | None:intergenic |
| AAGAGAGAAGATGGTTTGTT+TGG | 0.367598 | 1.2:+16782494 | None:intergenic |
| CTATAACTCCTGCTTCTTCA+AGG | 0.424048 | 1.2:-16783177 | None:intergenic |
| ATATTATTGCAGCATGAATT+GGG | 0.424242 | 1.2:+16783313 | MS.gene83077:intron |
| ACTTGTTGTTAGTTCACAAA+AGG | 0.426291 | 1.2:+16782981 | MS.gene83077:CDS |
| ATGCAAAGATACAATGAAAC+TGG | 0.427691 | 1.2:+16782537 | MS.gene83077:CDS |
| CAAGACATTGATGGCAATAT+GGG | 0.436846 | 1.2:+16782943 | MS.gene83077:CDS |
| AGTTTCATTAGCAAAAGATA+TGG | 0.452059 | 1.2:+16783343 | MS.gene83077:CDS |
| AGATATAAGCAAGACATTGA+TGG | 0.458567 | 1.2:+16782934 | MS.gene83077:CDS |
| TGGCAATATGGGTAATGAAC+TGG | 0.465445 | 1.2:+16782954 | MS.gene83077:CDS |
| AGTTGTTCCTTGACAAGTAA+GGG | 0.479458 | 1.2:-16783391 | None:intergenic |
| ACTGGTGGTCGTCAAGTTGT+AGG | 0.480361 | 1.2:+16782555 | MS.gene83077:CDS |
| CCAGAGATGTTTGTCAGCAT+TGG | 0.482593 | 1.2:+16783914 | MS.gene83077:CDS |
| GAGATGTTTGTCAGCATTGG+TGG | 0.494181 | 1.2:+16783917 | MS.gene83077:CDS |
| AAGTTGTTCCTTGACAAGTA+AGG | 0.495158 | 1.2:-16783392 | None:intergenic |
| TATAACTCCTGCTTCTTCAA+GGG | 0.500603 | 1.2:-16783176 | None:intergenic |
| CCAATGCTGACAAACATCTC+TGG | 0.500836 | 1.2:-16783914 | None:intergenic |
| AAATCTACGTACAAGAATAT+GGG | 0.505617 | 1.2:+16783432 | MS.gene83077:CDS |
| CATGTTTCCCTTACTTGTCA+AGG | 0.509328 | 1.2:+16783384 | MS.gene83077:CDS |
| CTTGTTGTTAGTTCACAAAA+GGG | 0.509904 | 1.2:+16782982 | MS.gene83077:CDS |
| TTGTCAGCATTGGTGGATGA+AGG | 0.547295 | 1.2:+16783924 | MS.gene83077:CDS |
| TCAAGGAACAACTTGAACAC+TGG | 0.547860 | 1.2:+16783401 | MS.gene83077:CDS |
| AGAGAATCCCTTGAAGAAGC+AGG | 0.547892 | 1.2:+16783169 | MS.gene83077:CDS |
| CTGGTGGTCGTCAAGTTGTA+GGG | 0.551311 | 1.2:+16782556 | MS.gene83077:CDS |
| TGCAGCATGAATTGGGCGAA+TGG | 0.558114 | 1.2:+16783320 | MS.gene83077:intron |
| TGTCTTGCTTATATCTGTAT+GGG | 0.559350 | 1.2:-16782927 | None:intergenic |
| AGATATGGTACATATTATGA+AGG | 0.566362 | 1.2:+16783358 | MS.gene83077:CDS |
| TCAATCATTTATGTTTCCAA+AGG | 0.579468 | 1.2:+16783005 | MS.gene83077:CDS |
| GTTTGGTATCTCGTTCTGGA+AGG | 0.579600 | 1.2:+16782511 | MS.gene83077:CDS |
| AGGAGTTATAGGAATTGTTG+AGG | 0.587265 | 1.2:+16783189 | MS.gene83077:CDS |
| CAAAGATACAATGAAACTGG+TGG | 0.665224 | 1.2:+16782540 | MS.gene83077:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAAAACAAATTAATAAA+AGG | - | chr1.2:16783104-16783123 | None:intergenic | 10.0% |
| !! | TAATTAATGAATAATAATGA+TGG | - | chr1.2:16782849-16782868 | None:intergenic | 10.0% |
| !!! | AAATGAAAATTTTTTATTGA+CGG | - | chr1.2:16783671-16783690 | None:intergenic | 10.0% |
| !!! | AGCATTATTTTTATAAATTG+TGG | - | chr1.2:16783765-16783784 | None:intergenic | 15.0% |
| !!! | TATTAATTTGTTTTGATTCA+GGG | + | chr1.2:16783106-16783125 | MS.gene83077:intron | 15.0% |
| !!! | TGCTTAATTTTTTTTAATCT+TGG | + | chr1.2:16782807-16782826 | MS.gene83077:intron | 15.0% |
| !!! | TTATTAATTTGTTTTGATTC+AGG | + | chr1.2:16783105-16783124 | MS.gene83077:intron | 15.0% |
| !! | TCTAATCTAGATATATCAAA+AGG | - | chr1.2:16782749-16782768 | None:intergenic | 20.0% |
| !! | TTAGAAATATAATCTACGAT+CGG | + | chr1.2:16783538-16783557 | MS.gene83077:intron | 20.0% |
| !!! | ATTGAAATTGTTTCTGAATT+AGG | + | chr1.2:16783475-16783494 | MS.gene83077:intron | 20.0% |
| !!! | TGTTGAGGTATTTAATTAAT+TGG | + | chr1.2:16783204-16783223 | MS.gene83077:intron | 20.0% |
| ! | AAAATCTACGTACAAGAATA+TGG | + | chr1.2:16783431-16783450 | MS.gene83077:CDS | 25.0% |
| ! | AAATCTACGTACAAGAATAT+GGG | + | chr1.2:16783432-16783451 | MS.gene83077:CDS | 25.0% |
| ! | AGATATGGTACATATTATGA+AGG | + | chr1.2:16783358-16783377 | MS.gene83077:CDS | 25.0% |
| ! | AGTTTCATTAGCAAAAGATA+TGG | + | chr1.2:16783343-16783362 | MS.gene83077:CDS | 25.0% |
| ! | ATATTATTGCAGCATGAATT+GGG | + | chr1.2:16783313-16783332 | MS.gene83077:intron | 25.0% |
| ! | ATCTAGATATATCAAAAGGA+TGG | - | chr1.2:16782745-16782764 | None:intergenic | 25.0% |
| ! | ATTAATATGTCCAATTGTGT+AGG | + | chr1.2:16782899-16782918 | MS.gene83077:intron | 25.0% |
| ! | CATAAAGAGATTGCTTAATT+AGG | + | chr1.2:16783047-16783066 | MS.gene83077:intron | 25.0% |
| ! | TCAATCATTTATGTTTCCAA+AGG | + | chr1.2:16783005-16783024 | MS.gene83077:CDS | 25.0% |
| !! | CTTTAGATATACTTGTTGAT+AGG | + | chr1.2:16783950-16783969 | MS.gene83077:CDS | 25.0% |
| !!! | GTGAAGACATGTTTTTTATA+TGG | + | chr1.2:16783790-16783809 | MS.gene83077:intron | 25.0% |
| !!! | TATGTATAGTTTTATCAGTC+TGG | + | chr1.2:16782623-16782642 | MS.gene83077:intron | 25.0% |
| AAAGAAACTTCGTTATGATG+AGG | + | chr1.2:16783512-16783531 | MS.gene83077:intron | 30.0% | |
| AACTGAATTTGATGTCCAAA+AGG | - | chr1.2:16782697-16782716 | None:intergenic | 30.0% | |
| AGATATAAGCAAGACATTGA+TGG | + | chr1.2:16782934-16782953 | MS.gene83077:CDS | 30.0% | |
| ATGCAAAGATACAATGAAAC+TGG | + | chr1.2:16782537-16782556 | MS.gene83077:CDS | 30.0% | |
| ATGTCTTGCTTATATCTGTA+TGG | - | chr1.2:16782931-16782950 | None:intergenic | 30.0% | |
| CATATTATTGCAGCATGAAT+TGG | + | chr1.2:16783312-16783331 | MS.gene83077:intron | 30.0% | |
| CATTTATGTTTCCAAAGGTA+GGG | + | chr1.2:16783010-16783029 | MS.gene83077:intron | 30.0% | |
| TCATTTATGTTTCCAAAGGT+AGG | + | chr1.2:16783009-16783028 | MS.gene83077:intron | 30.0% | |
| TGTCTTGCTTATATCTGTAT+GGG | - | chr1.2:16782930-16782949 | None:intergenic | 30.0% | |
| TTAGTCAAACATGACTGATA+TGG | - | chr1.2:16783571-16783590 | None:intergenic | 30.0% | |
| !! | ACTTGTTGTTAGTTCACAAA+AGG | + | chr1.2:16782981-16783000 | MS.gene83077:CDS | 30.0% |
| !! | CTTGTTGTTAGTTCACAAAA+GGG | + | chr1.2:16782982-16783001 | MS.gene83077:CDS | 30.0% |
| !!! | TAATTTGTTTTGATTCAGGG+TGG | + | chr1.2:16783109-16783128 | MS.gene83077:intron | 30.0% |
| AAGTTGTTCCTTGACAAGTA+AGG | - | chr1.2:16783395-16783414 | None:intergenic | 35.0% | |
| AGGAGTTATAGGAATTGTTG+AGG | + | chr1.2:16783189-16783208 | MS.gene83077:CDS | 35.0% | |
| AGTTGTTCCTTGACAAGTAA+GGG | - | chr1.2:16783394-16783413 | None:intergenic | 35.0% | |
| ATGAAAGAAATCCCTACCTT+TGG | - | chr1.2:16783024-16783043 | None:intergenic | 35.0% | |
| CAAAGATACAATGAAACTGG+TGG | + | chr1.2:16782540-16782559 | MS.gene83077:CDS | 35.0% | |
| TATAACTCCTGCTTCTTCAA+GGG | - | chr1.2:16783179-16783198 | None:intergenic | 35.0% | |
| TTAGTCTGATCTCGATCTAA+CGG | - | chr1.2:16782652-16782671 | None:intergenic | 35.0% | |
| ! | CAAGACATTGATGGCAATAT+GGG | + | chr1.2:16782943-16782962 | MS.gene83077:CDS | 35.0% |
| ! | CTTGTACGTAGATTTTTCTC+AGG | - | chr1.2:16783427-16783446 | None:intergenic | 35.0% |
| ! | GAATACGCTTTCTTTCCTTT+TGG | + | chr1.2:16782679-16782698 | MS.gene83077:intron | 35.0% |
| CATGTTTCCCTTACTTGTCA+AGG | + | chr1.2:16783384-16783403 | MS.gene83077:CDS | 40.0% | |
| CTATAACTCCTGCTTCTTCA+AGG | - | chr1.2:16783180-16783199 | None:intergenic | 40.0% | |
| CTTGAAGAAGCAGGAGTTAT+AGG | + | chr1.2:16783178-16783197 | MS.gene83077:CDS | 40.0% | |
| GTTTGTTTGGTATCTCGTTC+TGG | + | chr1.2:16782507-16782526 | MS.gene83077:CDS | 40.0% | |
| TCAAGGAACAACTTGAACAC+TGG | + | chr1.2:16783401-16783420 | MS.gene83077:CDS | 40.0% | |
| TGGCAATATGGGTAATGAAC+TGG | + | chr1.2:16782954-16782973 | MS.gene83077:CDS | 40.0% | |
| ! | GCAAGACATTGATGGCAATA+TGG | + | chr1.2:16782942-16782961 | MS.gene83077:CDS | 40.0% |
| !!! | TGTTTTGATTCAGGGTGGAT+GGG | + | chr1.2:16783114-16783133 | MS.gene83077:intron | 40.0% |
| !!! | TTGTTTTGATTCAGGGTGGA+TGG | + | chr1.2:16783113-16783132 | MS.gene83077:intron | 40.0% |
| AGAGAATCCCTTGAAGAAGC+AGG | + | chr1.2:16783169-16783188 | MS.gene83077:CDS | 45.0% | |
| ATGGGATGCACCTACACAAT+TGG | - | chr1.2:16782912-16782931 | None:intergenic | 45.0% | |
| CCAATGCTGACAAACATCTC+TGG | - | chr1.2:16783917-16783936 | None:intergenic | 45.0% | |
| CCAGAGATGTTTGTCAGCAT+TGG | + | chr1.2:16783914-16783933 | MS.gene83077:CDS | 45.0% | |
| ! | GAGATGTTTGTCAGCATTGG+TGG | + | chr1.2:16783917-16783936 | MS.gene83077:CDS | 45.0% |
| ! | GTTTGGTATCTCGTTCTGGA+AGG | + | chr1.2:16782511-16782530 | MS.gene83077:CDS | 45.0% |
| !! | TTGTCAGCATTGGTGGATGA+AGG | + | chr1.2:16783924-16783943 | MS.gene83077:CDS | 45.0% |
| TGCAGCATGAATTGGGCGAA+TGG | + | chr1.2:16783320-16783339 | MS.gene83077:intron | 50.0% | |
| ! | ACTGGTGGTCGTCAAGTTGT+AGG | + | chr1.2:16782555-16782574 | MS.gene83077:CDS | 50.0% |
| ! | CTGGTGGTCGTCAAGTTGTA+GGG | + | chr1.2:16782556-16782575 | MS.gene83077:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 16782504 | 16784002 | 16782504 | ID=MS.gene83077 |
| chr1.2 | mRNA | 16782504 | 16784002 | 16782504 | ID=MS.gene83077.t1;Parent=MS.gene83077 |
| chr1.2 | exon | 16782504 | 16782577 | 16782504 | ID=MS.gene83077.t1.exon1;Parent=MS.gene83077.t1 |
| chr1.2 | CDS | 16782504 | 16782577 | 16782504 | ID=cds.MS.gene83077.t1;Parent=MS.gene83077.t1 |
| chr1.2 | exon | 16782921 | 16783026 | 16782921 | ID=MS.gene83077.t1.exon2;Parent=MS.gene83077.t1 |
| chr1.2 | CDS | 16782921 | 16783026 | 16782921 | ID=cds.MS.gene83077.t1;Parent=MS.gene83077.t1 |
| chr1.2 | exon | 16783127 | 16783210 | 16783127 | ID=MS.gene83077.t1.exon3;Parent=MS.gene83077.t1 |
| chr1.2 | CDS | 16783127 | 16783210 | 16783127 | ID=cds.MS.gene83077.t1;Parent=MS.gene83077.t1 |
| chr1.2 | exon | 16783325 | 16783453 | 16783325 | ID=MS.gene83077.t1.exon4;Parent=MS.gene83077.t1 |
| chr1.2 | CDS | 16783325 | 16783453 | 16783325 | ID=cds.MS.gene83077.t1;Parent=MS.gene83077.t1 |
| chr1.2 | exon | 16783898 | 16784002 | 16783898 | ID=MS.gene83077.t1.exon5;Parent=MS.gene83077.t1 |
| chr1.2 | CDS | 16783898 | 16784002 | 16783898 | ID=cds.MS.gene83077.t1;Parent=MS.gene83077.t1 |
| Gene Sequence |
| Protein sequence |
