Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83141.t1 | XP_003607769.1 | 95.4 | 130 | 6 | 0 | 1 | 130 | 210 | 339 | 5.50E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83141.t1 | G7JGB7 | 95.4 | 130 | 6 | 0 | 1 | 130 | 210 | 339 | 4.0e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene83141 | -0.814119 | 1.81E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene83141 | -0.810944 | 8.96E-51 | -1.69E-46 |
| MS.gene050763 | MS.gene83141 | -0.812893 | 3.37E-51 | -1.69E-46 |
| MS.gene052673 | MS.gene83141 | 0.820069 | 8.33E-53 | -1.69E-46 |
| MS.gene053054 | MS.gene83141 | 0.807632 | 4.60E-50 | -1.69E-46 |
| MS.gene05468 | MS.gene83141 | -0.802411 | 5.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAGAGTTGTTTCTGATAG+TGG | 0.354480 | 4.2:-27083131 | MS.gene83141:CDS |
| TAGTGGTTCATGGAGGGATT+GGG | 0.370675 | 4.2:-27083114 | MS.gene83141:CDS |
| TTGTTCTTTGCTTCTTTGGA+AGG | 0.386772 | 4.2:-27083182 | MS.gene83141:CDS |
| TTGTTTCTGATAGTGGTTCA+TGG | 0.410409 | 4.2:-27083124 | MS.gene83141:CDS |
| ATAGTGGTTCATGGAGGGAT+TGG | 0.411097 | 4.2:-27083115 | MS.gene83141:CDS |
| GAACAAAAGGCAAGAAGCTC+CGG | 0.446232 | 4.2:+27083199 | None:intergenic |
| CCAAAGAAGCAAAGAACAAA+AGG | 0.464801 | 4.2:+27083186 | None:intergenic |
| TTCTGATAGTGGTTCATGGA+GGG | 0.509452 | 4.2:-27083120 | MS.gene83141:CDS |
| TAAGGCTGTACATGGTGAAC+TGG | 0.533194 | 4.2:-27083000 | MS.gene83141:CDS |
| GGAACTTTGCCATCATCAAA+AGG | 0.535133 | 4.2:+27082933 | None:intergenic |
| TGAACTGGCGGAGGAGATTG+TGG | 0.538035 | 4.2:-27082985 | MS.gene83141:CDS |
| GAACACTCCGGCGAAGTGGT+TGG | 0.543138 | 4.2:+27083246 | None:intergenic |
| CTCCTCAGTCATCGCCGTAG+TGG | 0.558626 | 4.2:-27083285 | None:intergenic |
| CTTTGGAAGGAATTATGTTG+TGG | 0.567519 | 4.2:-27083169 | MS.gene83141:CDS |
| ACGGAATCCAACCACTTCGC+CGG | 0.587915 | 4.2:-27083253 | MS.gene83141:CDS |
| CGTGCTGTATATGTACTGTA+AGG | 0.602621 | 4.2:-27083018 | MS.gene83141:CDS |
| TGTACATGGTGAACTGGCGG+AGG | 0.604228 | 4.2:-27082994 | MS.gene83141:CDS |
| AAGCATCAACACTGTCATCA+CGG | 0.615136 | 4.2:+27083061 | None:intergenic |
| TTTCTGATAGTGGTTCATGG+AGG | 0.616797 | 4.2:-27083121 | MS.gene83141:CDS |
| ATGTACTGTAAGGCTGTACA+TGG | 0.618850 | 4.2:-27083008 | MS.gene83141:CDS |
| GGCTGTACATGGTGAACTGG+CGG | 0.620056 | 4.2:-27082997 | MS.gene83141:CDS |
| AGATTGTGGAGGAGTTTGCA+TGG | 0.621100 | 4.2:-27082971 | MS.gene83141:CDS |
| GAAGGAATTATGTTGTGGAG+TGG | 0.623006 | 4.2:-27083164 | MS.gene83141:CDS |
| AGATGAACACTCCGGCGAAG+TGG | 0.626387 | 4.2:+27083242 | None:intergenic |
| CGGAGTGTTCATCTGATGAA+CGG | 0.631386 | 4.2:-27083233 | MS.gene83141:CDS |
| GCCGTAGTGGAAAACACCTA+CGG | 0.636102 | 4.2:-27083272 | MS.gene83141:CDS |
| GCGAAGTGGTTGGATTCCGT+AGG | 0.638817 | 4.2:+27083256 | None:intergenic |
| CATATACAGCACGGTGTCCG+CGG | 0.662637 | 4.2:+27083028 | None:intergenic |
| TGATGGCAAAGTTCCTCATG+TGG | 0.667427 | 4.2:-27082925 | MS.gene83141:CDS |
| ATGAACGGAATGAAATACAC+CGG | 0.680070 | 4.2:-27083218 | MS.gene83141:CDS |
| CGTTCATCAGATGAACACTC+CGG | 0.693640 | 4.2:+27083234 | None:intergenic |
| GATGCTTTACAACGCCGCCG+CGG | 0.710091 | 4.2:-27083045 | MS.gene83141:CDS |
| ACTGGCGGAGGAGATTGTGG+AGG | 0.713390 | 4.2:-27082982 | MS.gene83141:CDS |
| ATACAGCACGGTGTCCGCGG+CGG | 0.729974 | 4.2:+27083031 | None:intergenic |
| CTTACAGTACATATACAGCA+CGG | 0.737948 | 4.2:+27083019 | None:intergenic |
| TGTACAACAGAAACCACATG+AGG | 0.740386 | 4.2:+27082912 | None:intergenic |
| CATCAACACTGTCATCACGG+CGG | 0.807246 | 4.2:+27083064 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAACGGAATGAAATACAC+CGG | - | chr4.2:27082964-27082983 | MS.gene83141:CDS | 35.0% | |
| CCAAAGAAGCAAAGAACAAA+AGG | + | chr4.2:27082999-27083018 | None:intergenic | 35.0% | |
| CTTACAGTACATATACAGCA+CGG | + | chr4.2:27083166-27083185 | None:intergenic | 35.0% | |
| TTGTTCTTTGCTTCTTTGGA+AGG | - | chr4.2:27083000-27083019 | MS.gene83141:CDS | 35.0% | |
| ! | CCTTTTGTTCTTTGCTTCTT+TGG | - | chr4.2:27082996-27083015 | MS.gene83141:CDS | 35.0% |
| ! | CTTTGGAAGGAATTATGTTG+TGG | - | chr4.2:27083013-27083032 | MS.gene83141:CDS | 35.0% |
| !! | TTGTTTCTGATAGTGGTTCA+TGG | - | chr4.2:27083058-27083077 | MS.gene83141:CDS | 35.0% |
| AAGCATCAACACTGTCATCA+CGG | + | chr4.2:27083124-27083143 | None:intergenic | 40.0% | |
| ATGTACTGTAAGGCTGTACA+TGG | - | chr4.2:27083174-27083193 | MS.gene83141:CDS | 40.0% | |
| CGTGCTGTATATGTACTGTA+AGG | - | chr4.2:27083164-27083183 | MS.gene83141:CDS | 40.0% | |
| GAAGGAATTATGTTGTGGAG+TGG | - | chr4.2:27083018-27083037 | MS.gene83141:CDS | 40.0% | |
| GGAACTTTGCCATCATCAAA+AGG | + | chr4.2:27083252-27083271 | None:intergenic | 40.0% | |
| TGTACAACAGAAACCACATG+AGG | + | chr4.2:27083273-27083292 | None:intergenic | 40.0% | |
| ! | GTGAGTTTGCCTTTTGATGA+TGG | - | chr4.2:27083240-27083259 | MS.gene83141:CDS | 40.0% |
| !! | GCTAGAGTTGTTTCTGATAG+TGG | - | chr4.2:27083051-27083070 | MS.gene83141:CDS | 40.0% |
| !! | TTCTGATAGTGGTTCATGGA+GGG | - | chr4.2:27083062-27083081 | MS.gene83141:CDS | 40.0% |
| !! | TTTCTGATAGTGGTTCATGG+AGG | - | chr4.2:27083061-27083080 | MS.gene83141:CDS | 40.0% |
| AGATTGTGGAGGAGTTTGCA+TGG | - | chr4.2:27083211-27083230 | MS.gene83141:CDS | 45.0% | |
| ATAGTGGTTCATGGAGGGAT+TGG | - | chr4.2:27083067-27083086 | MS.gene83141:CDS | 45.0% | |
| CGTTCATCAGATGAACACTC+CGG | + | chr4.2:27082951-27082970 | None:intergenic | 45.0% | |
| GAACAAAAGGCAAGAAGCTC+CGG | + | chr4.2:27082986-27083005 | None:intergenic | 45.0% | |
| TAAGGCTGTACATGGTGAAC+TGG | - | chr4.2:27083182-27083201 | MS.gene83141:CDS | 45.0% | |
| TAGTGGTTCATGGAGGGATT+GGG | - | chr4.2:27083068-27083087 | MS.gene83141:CDS | 45.0% | |
| TGATGGCAAAGTTCCTCATG+TGG | - | chr4.2:27083257-27083276 | MS.gene83141:CDS | 45.0% | |
| ! | ATGGAGGGATTGGGCTTTTA+TGG | - | chr4.2:27083077-27083096 | MS.gene83141:CDS | 45.0% |
| ! | CGGAGTGTTCATCTGATGAA+CGG | - | chr4.2:27082949-27082968 | MS.gene83141:CDS | 45.0% |
| ! | TCCGTAGGTGTTTTCCACTA+CGG | + | chr4.2:27082914-27082933 | None:intergenic | 45.0% |
| CATCAACACTGTCATCACGG+CGG | + | chr4.2:27083121-27083140 | None:intergenic | 50.0% | |
| ! | GCCGTAGTGGAAAACACCTA+CGG | - | chr4.2:27082910-27082929 | MS.gene83141:CDS | 50.0% |
| ACGGAATCCAACCACTTCGC+CGG | - | chr4.2:27082929-27082948 | MS.gene83141:CDS | 55.0% | |
| AGATGAACACTCCGGCGAAG+TGG | + | chr4.2:27082943-27082962 | None:intergenic | 55.0% | |
| CATATACAGCACGGTGTCCG+CGG | + | chr4.2:27083157-27083176 | None:intergenic | 55.0% | |
| GCGAAGTGGTTGGATTCCGT+AGG | + | chr4.2:27082929-27082948 | None:intergenic | 55.0% | |
| GGCTGTACATGGTGAACTGG+CGG | - | chr4.2:27083185-27083204 | MS.gene83141:CDS | 55.0% | |
| TGAACTGGCGGAGGAGATTG+TGG | - | chr4.2:27083197-27083216 | MS.gene83141:CDS | 55.0% | |
| TGTACATGGTGAACTGGCGG+AGG | - | chr4.2:27083188-27083207 | MS.gene83141:CDS | 55.0% | |
| ACTGGCGGAGGAGATTGTGG+AGG | - | chr4.2:27083200-27083219 | MS.gene83141:CDS | 60.0% | |
| GAACACTCCGGCGAAGTGGT+TGG | + | chr4.2:27082939-27082958 | None:intergenic | 60.0% | |
| GATGCTTTACAACGCCGCCG+CGG | - | chr4.2:27083137-27083156 | MS.gene83141:CDS | 60.0% | |
| ATACAGCACGGTGTCCGCGG+CGG | + | chr4.2:27083154-27083173 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 27082905 | 27083299 | 27082905 | ID=MS.gene83141 |
| chr4.2 | mRNA | 27082905 | 27083299 | 27082905 | ID=MS.gene83141.t1;Parent=MS.gene83141 |
| chr4.2 | exon | 27082905 | 27083299 | 27082905 | ID=MS.gene83141.t1.exon1;Parent=MS.gene83141.t1 |
| chr4.2 | CDS | 27082905 | 27083299 | 27082905 | ID=cds.MS.gene83141.t1;Parent=MS.gene83141.t1 |
| Gene Sequence |
| Protein sequence |
