Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83306.t1 | XP_003619776.1 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 2.60E-76 | 294.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83306.t1 | Q6L4D2 | 45.5 | 143 | 64 | 3 | 8 | 149 | 12 | 141 | 4.1e-22 | 105.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83306.t1 | G7KPB9 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 1.9e-76 | 294.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050444 | MS.gene83306 | -0.810554 | 1.09E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene83306 | -0.807607 | 4.66E-50 | -1.69E-46 |
| MS.gene056387 | MS.gene83306 | -0.810713 | 1.01E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene83306 | -0.807527 | 4.84E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene83306 | -0.819479 | 1.14E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83306.t1 | MTR_6g068860 | 97.452 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 3.73e-110 | 310 |
| MS.gene83306.t1 | MTR_2g049340 | 74.825 | 143 | 36 | 0 | 1 | 143 | 6 | 148 | 3.37e-70 | 209 |
| MS.gene83306.t1 | MTR_4g117600 | 45.985 | 137 | 74 | 0 | 16 | 152 | 21 | 157 | 4.12e-35 | 120 |
| MS.gene83306.t1 | MTR_1g094035 | 43.506 | 154 | 72 | 5 | 2 | 154 | 6 | 145 | 8.74e-28 | 102 |
| MS.gene83306.t1 | MTR_4g117600 | 46.939 | 98 | 52 | 0 | 16 | 113 | 21 | 118 | 3.73e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83306.t1 | AT5G46530 | 71.812 | 149 | 42 | 0 | 1 | 149 | 6 | 154 | 1.75e-76 | 225 |
| MS.gene83306.t1 | AT1G29520 | 78.523 | 149 | 32 | 0 | 1 | 149 | 6 | 154 | 8.97e-76 | 223 |
| MS.gene83306.t1 | AT5G18970 | 43.506 | 154 | 87 | 0 | 2 | 155 | 7 | 160 | 8.39e-38 | 127 |
| MS.gene83306.t1 | AT1G04560 | 44.231 | 156 | 73 | 4 | 2 | 156 | 7 | 149 | 2.01e-31 | 111 |
Find 29 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCCCATTGGGAAGAAAA+TGG | 0.238328 | 6.1:-62979549 | None:intergenic |
| TGCATGTATGTCATTGTTCT+AGG | 0.248721 | 6.1:+62979276 | MS.gene83306:CDS |
| CTCTACATAGTTGCAATTCA+TGG | 0.294119 | 6.1:+62980392 | MS.gene83306:CDS |
| GGTGCTGCATCGACAATTTC+AGG | 0.318250 | 6.1:+62979620 | MS.gene83306:CDS |
| TGCTGCTTCAATTGCTACCT+TGG | 0.341425 | 6.1:+62979685 | MS.gene83306:CDS |
| CCAATGGGAAATGCTTCAAC+TGG | 0.374176 | 6.1:+62979560 | MS.gene83306:CDS |
| CTTCAATTGCTACCTTGGCT+TGG | 0.378873 | 6.1:+62979690 | MS.gene83306:CDS |
| GACTCTTACACTGCTTGCCA+TGG | 0.388463 | 6.1:+62979712 | MS.gene83306:CDS |
| GTTACGATTTCTTTGTTAGC+TGG | 0.398205 | 6.1:+62979590 | MS.gene83306:CDS |
| TCTTTGTTAGCTGGAGTAGT+TGG | 0.415192 | 6.1:+62979599 | MS.gene83306:CDS |
| TTGTTCTAGGCATTGGTGCA+TGG | 0.424644 | 6.1:+62979289 | MS.gene83306:CDS |
| CAGTTGAAGCATTTCCCATT+GGG | 0.433142 | 6.1:-62979559 | None:intergenic |
| TGAGCTGGAAGATTTAATTC+CGG | 0.439380 | 6.1:-62979518 | None:intergenic |
| TGTTCTAGGCATTGGTGCAT+GGG | 0.449144 | 6.1:+62979290 | MS.gene83306:CDS |
| ACTCTTACACTGCTTGCCAT+GGG | 0.453490 | 6.1:+62979713 | MS.gene83306:CDS |
| TTTCCCATTGGGAAGAAAAT+GGG | 0.461124 | 6.1:-62979548 | None:intergenic |
| GAGTCAAACATATCTGTTCA+TGG | 0.465531 | 6.1:+62979642 | MS.gene83306:CDS |
| TCAAATTAGAAATGCACGTC+TGG | 0.465623 | 6.1:+62980239 | MS.gene83306:CDS |
| CCAGTTGAAGCATTTCCCAT+TGG | 0.492597 | 6.1:-62979560 | None:intergenic |
| GATAATTATGAAAGCCTCCA+TGG | 0.495765 | 6.1:-62980351 | None:intergenic |
| TATGTCATTGTTCTAGGCAT+TGG | 0.506725 | 6.1:+62979282 | MS.gene83306:CDS |
| GTCCATAGCAACACTTCTCT+TGG | 0.510981 | 6.1:+62979242 | MS.gene83306:CDS |
| CACCAAGAGAAGTGTTGCTA+TGG | 0.525202 | 6.1:-62979244 | None:intergenic |
| TTAACTTCTGCAGATTTGCA+TGG | 0.530112 | 6.1:+62980202 | MS.gene83306:intron |
| ATGAATAGAGCAATAGATCA+TGG | 0.550315 | 6.1:+62979315 | MS.gene83306:CDS |
| AGAGAAGTTGTGTAACTGAA+AGG | 0.568727 | 6.1:-62980373 | None:intergenic |
| CTCTTACACTGCTTGCCATG+GGG | 0.615745 | 6.1:+62979714 | MS.gene83306:CDS |
| GTAGCAATTGAAGCAGCAGA+TGG | 0.618253 | 6.1:-62979680 | None:intergenic |
| CAGTGTAAGAGTCCAAGCCA+AGG | 0.634224 | 6.1:-62979702 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAACTATAAAAATCAA+AGG | + | chr6.1:62979913-62979932 | MS.gene83306:intron | 10.0% |
| !!! | TTCTTTTTATTACTATTATT+TGG | - | chr6.1:62979783-62979802 | None:intergenic | 10.0% |
| !!! | GAATCATTTAATAATTTTCT+TGG | + | chr6.1:62980275-62980294 | MS.gene83306:intron | 15.0% |
| !!! | GTTTTAATTGAAAACAAATT+TGG | - | chr6.1:62980135-62980154 | None:intergenic | 15.0% |
| !!! | TTTATTTTATCCATTTCTAT+AGG | + | chr6.1:62979844-62979863 | MS.gene83306:intron | 15.0% |
| !!! | TTTTTATTACTATTATTTGG+AGG | - | chr6.1:62979780-62979799 | None:intergenic | 15.0% |
| !!! | TTCTGACATTCTAAAATATT+TGG | - | chr6.1:62979383-62979402 | None:intergenic | 20.0% |
| ! | ATTACTATTATTTGGAGGTA+TGG | - | chr6.1:62979775-62979794 | None:intergenic | 25.0% |
| ! | CGATGAATTTGGTAAAATTA+TGG | + | chr6.1:62980048-62980067 | MS.gene83306:intron | 25.0% |
| ! | TTAGAATGTCAGAAAAACAA+TGG | + | chr6.1:62979389-62979408 | MS.gene83306:intron | 25.0% |
| !!! | ATAGATCATGGTTTTATCAT+AGG | + | chr6.1:62979327-62979346 | MS.gene83306:CDS | 25.0% |
| !!! | ATTTTGTTATGAAATTACGC+AGG | + | chr6.1:62979494-62979513 | MS.gene83306:intron | 25.0% |
| ATGAATAGAGCAATAGATCA+TGG | + | chr6.1:62979315-62979334 | MS.gene83306:CDS | 30.0% | |
| ATTTGGCAAATGTGAAAGAT+AGG | - | chr6.1:62979366-62979385 | None:intergenic | 30.0% | |
| CTATCAATCACCTATAGAAA+TGG | - | chr6.1:62979857-62979876 | None:intergenic | 30.0% | |
| ! | AAATCAAAGGAGCGTATTAA+AGG | + | chr6.1:62979926-62979945 | MS.gene83306:intron | 30.0% |
| ! | AATCAAAGGAGCGTATTAAA+GGG | + | chr6.1:62979927-62979946 | MS.gene83306:intron | 30.0% |
| !!! | TCTATTTTTGCAGAAAACCA+TGG | + | chr6.1:62980334-62980353 | MS.gene83306:intron | 30.0% |
| AAAATGGGTGAAAAATGAGC+TGG | - | chr6.1:62979536-62979555 | None:intergenic | 35.0% | |
| AGAGAAGTTGTGTAACTGAA+AGG | - | chr6.1:62980376-62980395 | None:intergenic | 35.0% | |
| ATTTCCCATTGGGAAGAAAA+TGG | - | chr6.1:62979552-62979571 | None:intergenic | 35.0% | |
| CTCTACATAGTTGCAATTCA+TGG | + | chr6.1:62980392-62980411 | MS.gene83306:CDS | 35.0% | |
| GAGTCAAACATATCTGTTCA+TGG | + | chr6.1:62979642-62979661 | MS.gene83306:CDS | 35.0% | |
| GATAATTATGAAAGCCTCCA+TGG | - | chr6.1:62980354-62980373 | None:intergenic | 35.0% | |
| GTTACGATTTCTTTGTTAGC+TGG | + | chr6.1:62979590-62979609 | MS.gene83306:CDS | 35.0% | |
| GTTTGGATTGACGATGAATT+TGG | + | chr6.1:62980037-62980056 | MS.gene83306:intron | 35.0% | |
| TAAGAGAGAAATTTACCCCA+TGG | - | chr6.1:62979732-62979751 | None:intergenic | 35.0% | |
| TCAAATTAGAAATGCACGTC+TGG | + | chr6.1:62980239-62980258 | MS.gene83306:CDS | 35.0% | |
| TGAGCTGGAAGATTTAATTC+CGG | - | chr6.1:62979521-62979540 | None:intergenic | 35.0% | |
| TGCATGTATGTCATTGTTCT+AGG | + | chr6.1:62979276-62979295 | MS.gene83306:CDS | 35.0% | |
| TTAACTTCTGCAGATTTGCA+TGG | + | chr6.1:62980202-62980221 | MS.gene83306:intron | 35.0% | |
| TTACCGGACTTGAATTACTT+CGG | + | chr6.1:62979464-62979483 | MS.gene83306:intron | 35.0% | |
| TTTCCCATTGGGAAGAAAAT+GGG | - | chr6.1:62979551-62979570 | None:intergenic | 35.0% | |
| ! | ATTTTTGCAGAAAACCATGG+AGG | + | chr6.1:62980337-62980356 | MS.gene83306:intron | 35.0% |
| ! | TATGTCATTGTTCTAGGCAT+TGG | + | chr6.1:62979282-62979301 | MS.gene83306:CDS | 35.0% |
| ! | TGCTCTTCTTTTATCTTACC+AGG | + | chr6.1:62980009-62980028 | MS.gene83306:intron | 35.0% |
| AACGTGTGAGTTAGGTTTAC+CGG | + | chr6.1:62979448-62979467 | MS.gene83306:intron | 40.0% | |
| ATGCCGAAGTAATTCAAGTC+CGG | - | chr6.1:62979470-62979489 | None:intergenic | 40.0% | |
| GTTATGAAATTACGCAGGTC+CGG | + | chr6.1:62979499-62979518 | MS.gene83306:intron | 40.0% | |
| TATCTTACCAGGTGCATGTT+TGG | + | chr6.1:62980020-62980039 | MS.gene83306:intron | 40.0% | |
| TCTTTGTTAGCTGGAGTAGT+TGG | + | chr6.1:62979599-62979618 | MS.gene83306:CDS | 40.0% | |
| ! | ATTTTTCGCAGCTATTTGCG+TGG | + | chr6.1:62979424-62979443 | MS.gene83306:intron | 40.0% |
| ! | CAGTTGAAGCATTTCCCATT+GGG | - | chr6.1:62979562-62979581 | None:intergenic | 40.0% |
| ! | TCACCCATTTTCTTCCCAAT+GGG | + | chr6.1:62979545-62979564 | MS.gene83306:CDS | 40.0% |
| ! | TTCACCCATTTTCTTCCCAA+TGG | + | chr6.1:62979544-62979563 | MS.gene83306:CDS | 40.0% |
| ACTCTTACACTGCTTGCCAT+GGG | + | chr6.1:62979713-62979732 | MS.gene83306:CDS | 45.0% | |
| CCAATGGGAAATGCTTCAAC+TGG | + | chr6.1:62979560-62979579 | MS.gene83306:CDS | 45.0% | |
| CTTCAATTGCTACCTTGGCT+TGG | + | chr6.1:62979690-62979709 | MS.gene83306:CDS | 45.0% | |
| GTAGCAATTGAAGCAGCAGA+TGG | - | chr6.1:62979683-62979702 | None:intergenic | 45.0% | |
| GTCCATAGCAACACTTCTCT+TGG | + | chr6.1:62979242-62979261 | MS.gene83306:CDS | 45.0% | |
| ! | CCAGTTGAAGCATTTCCCAT+TGG | - | chr6.1:62979563-62979582 | None:intergenic | 45.0% |
| ! | TGCTGCTTCAATTGCTACCT+TGG | + | chr6.1:62979685-62979704 | MS.gene83306:CDS | 45.0% |
| !! | CACCAAGAGAAGTGTTGCTA+TGG | - | chr6.1:62979247-62979266 | None:intergenic | 45.0% |
| !! | TGTTCTAGGCATTGGTGCAT+GGG | + | chr6.1:62979290-62979309 | MS.gene83306:CDS | 45.0% |
| !! | TTGTTCTAGGCATTGGTGCA+TGG | + | chr6.1:62979289-62979308 | MS.gene83306:CDS | 45.0% |
| CAGTGTAAGAGTCCAAGCCA+AGG | - | chr6.1:62979705-62979724 | None:intergenic | 50.0% | |
| CGTCAATCCAAACATGCACC+TGG | - | chr6.1:62980030-62980049 | None:intergenic | 50.0% | |
| CTCTTACACTGCTTGCCATG+GGG | + | chr6.1:62979714-62979733 | MS.gene83306:CDS | 50.0% | |
| GACTCTTACACTGCTTGCCA+TGG | + | chr6.1:62979712-62979731 | MS.gene83306:CDS | 50.0% | |
| ! | GGTGCTGCATCGACAATTTC+AGG | + | chr6.1:62979620-62979639 | MS.gene83306:CDS | 50.0% |
| ! | TGCGTGGTAACGTGTGAGTT+AGG | + | chr6.1:62979440-62979459 | MS.gene83306:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 62979237 | 62980442 | 62979237 | ID=MS.gene83306 |
| chr6.1 | mRNA | 62979237 | 62980442 | 62979237 | ID=MS.gene83306.t1;Parent=MS.gene83306 |
| chr6.1 | exon | 62979237 | 62979348 | 62979237 | ID=MS.gene83306.t1.exon1;Parent=MS.gene83306.t1 |
| chr6.1 | CDS | 62979237 | 62979348 | 62979237 | ID=cds.MS.gene83306.t1;Parent=MS.gene83306.t1 |
| chr6.1 | exon | 62979516 | 62979735 | 62979516 | ID=MS.gene83306.t1.exon2;Parent=MS.gene83306.t1 |
| chr6.1 | CDS | 62979516 | 62979735 | 62979516 | ID=cds.MS.gene83306.t1;Parent=MS.gene83306.t1 |
| chr6.1 | exon | 62980215 | 62980260 | 62980215 | ID=MS.gene83306.t1.exon3;Parent=MS.gene83306.t1 |
| chr6.1 | CDS | 62980215 | 62980260 | 62980215 | ID=cds.MS.gene83306.t1;Parent=MS.gene83306.t1 |
| chr6.1 | exon | 62980347 | 62980442 | 62980347 | ID=MS.gene83306.t1.exon4;Parent=MS.gene83306.t1 |
| chr6.1 | CDS | 62980347 | 62980442 | 62980347 | ID=cds.MS.gene83306.t1;Parent=MS.gene83306.t1 |
| Gene Sequence |
| Protein sequence |
