AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84543


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84543.t1 MTR_3g061590 98.425 254 4 0 17 270 6 259 0.0 513
MS.gene84543.t1 MTR_8g073580 50.980 255 113 4 23 270 40 289 6.15e-74 228
MS.gene84543.t1 MTR_8g073580 53.110 209 87 3 69 270 38 242 4.81e-71 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84543.t1 AT3G20890 60.274 292 78 4 15 270 3 292 1.41e-109 318
MS.gene84543.t1 AT3G20890 58.128 203 52 1 101 270 27 229 1.83e-78 237
MS.gene84543.t1 AT5G66010 49.587 242 109 5 21 258 10 242 1.22e-73 226
MS.gene84543.t1 AT5G66010 48.630 146 70 2 117 258 1 145 2.03e-43 145

Find 75 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTTATTGACTTCAATTT+TGG 0.156518 3.1:-46261152 MS.gene84543:CDS
CGGTGAAGGCTTCTGTGTTT+TGG 0.182250 3.1:-46260840 MS.gene84543:CDS
ATCCACATTGTAATGAATTC+AGG 0.251651 3.1:-46260524 MS.gene84543:CDS
TCATCCTTATTAGCAGAAAA+TGG 0.345506 3.1:+46260590 None:intergenic
AGATGGATTGTACATATAAC+TGG 0.382208 3.1:+46261018 None:intergenic
ACTTTGCTCTTCAAAGAAAC+AGG 0.406141 3.1:-46260799 MS.gene84543:CDS
TGCTAATAAGGATGACATAA+TGG 0.410596 3.1:-46260582 MS.gene84543:CDS
TCCATCTCACCATGCGTTGA+AGG 0.422533 3.1:+46260392 None:intergenic
CTACAAGGCAGTTGCAAATG+AGG 0.438613 3.1:-46260723 MS.gene84543:CDS
CGCAGTCGAACAACAGGAAA+TGG 0.442842 3.1:+46260962 None:intergenic
AGACCGCGCAGTCGAACAAC+AGG 0.447152 3.1:+46260956 None:intergenic
CTTCATCATATGATTTAGCT+CGG 0.461470 3.1:+46260660 None:intergenic
TCTTCATTCTATGATTCCCC+TGG 0.465549 3.1:-46261043 MS.gene84543:CDS
ACCATCTGGGGAGGCTTATG+TGG 0.470749 3.1:-46260495 MS.gene84543:CDS
ATATGATGAAGGAAAAGATT+CGG 0.489693 3.1:-46260648 MS.gene84543:CDS
CTTCAAGGAGTATGTGTTGT+CGG 0.500765 3.1:-46260555 MS.gene84543:CDS
CACAGGAGTGTTGCGACTGA+GGG 0.505697 3.1:-46260618 MS.gene84543:CDS
ATGTACAATCCATCTCCATA+TGG 0.508179 3.1:-46261010 MS.gene84543:CDS
CTCGGGGAGCACTACGACGA+GGG 0.508597 3.1:+46260678 None:intergenic
ACAGGAAATGGTGGAGGAGG+AGG 0.509420 3.1:+46260974 None:intergenic
TCCACATTGTAATGAATTCA+GGG 0.513953 3.1:-46260523 MS.gene84543:CDS
TTCATCATATGATTTAGCTC+GGG 0.517226 3.1:+46260661 None:intergenic
CGAGCTAAATCATATGATGA+AGG 0.518042 3.1:-46260659 MS.gene84543:CDS
ATCAGTTTCAGTGCAATCAA+AGG 0.523371 3.1:+46260931 None:intergenic
TGGTGGTGATGGGCGTGAAA+TGG 0.525727 3.1:-46261098 MS.gene84543:CDS
AAATTTGCTGATGGTGGTGA+TGG 0.527499 3.1:-46261109 MS.gene84543:CDS
CCATCTCACCATGCGTTGAA+GGG 0.532489 3.1:+46260393 None:intergenic
TGACATAATGGAGTTCTTCA+AGG 0.535855 3.1:-46260570 MS.gene84543:CDS
GATGTGGCCGAGTTCTTCCA+TGG 0.538399 3.1:-46260911 MS.gene84543:CDS
CCCTTCAACGCATGGTGAGA+TGG 0.538750 3.1:-46260393 MS.gene84543:CDS
GCAAAGGATAGAATGACGCT+TGG 0.544787 3.1:-46260437 MS.gene84543:CDS
CGTCATTCTATCCTTTGCCA+TGG 0.545218 3.1:+46260442 None:intergenic
TCAGTTTCAGTGCAATCAAA+GGG 0.547953 3.1:+46260932 None:intergenic
CAAAGGATAGAATGACGCTT+GGG 0.551160 3.1:-46260436 MS.gene84543:CDS
GGTTGGCTGACATATCCATA+TGG 0.556296 3.1:+46260995 None:intergenic
CAAAGAAACAGGCAGAACAT+AGG 0.560049 3.1:-46260788 MS.gene84543:CDS
TTCAGTAAATTTGCTGATGG+TGG 0.563548 3.1:-46261115 MS.gene84543:CDS
CTGACATATCCATATGGAGA+TGG 0.564910 3.1:+46261001 None:intergenic
TGTACATATAACTGGAACCA+GGG 0.571227 3.1:+46261026 None:intergenic
AGAGCAAAGTCAACTTGAAG+AGG 0.573046 3.1:+46260812 None:intergenic
ACAACAGGAAATGGTGGAGG+AGG 0.573282 3.1:+46260971 None:intergenic
TCCACATAAGCCTCCCCAGA+TGG 0.579302 3.1:+46260494 None:intergenic
GTCTAGACCATGGAAGAACT+CGG 0.581671 3.1:+46260904 None:intergenic
GAGCTATTCCCTTCAACGCA+TGG 0.586026 3.1:-46260401 MS.gene84543:CDS
CAAAACACAGAAGCCTTCAC+CGG 0.588242 3.1:+46260841 None:intergenic
TGATGATTCAAAAGCAGCCA+TGG 0.593984 3.1:-46260459 MS.gene84543:CDS
AATTTCAGTAAATTTGCTGA+TGG 0.594224 3.1:-46261118 MS.gene84543:CDS
ACACAGGAGTGTTGCGACTG+AGG 0.596079 3.1:-46260619 MS.gene84543:CDS
TCATCATATGATTTAGCTCG+GGG 0.596516 3.1:+46260662 None:intergenic
TTCAAAAGCAGCCATGGCAA+AGG 0.597698 3.1:-46260453 MS.gene84543:CDS
CGAACAACAGGAAATGGTGG+AGG 0.604157 3.1:+46260968 None:intergenic
GAAATGGTGGAGGAGGAGGT+TGG 0.608351 3.1:+46260978 None:intergenic
ATGGAAGATGCAATTTCAAG+AGG 0.609038 3.1:-46260374 MS.gene84543:CDS
TGATTGCACTGAAACTGATG+TGG 0.609327 3.1:-46260927 MS.gene84543:CDS
AAAGATTCGGCTGAGCACAC+AGG 0.623512 3.1:-46260635 MS.gene84543:CDS
ACGTCAGCGGACAGTTGACG+CGG 0.625189 3.1:-46261068 MS.gene84543:CDS
TCGGGGAGCACTACGACGAG+GGG 0.629479 3.1:+46260679 None:intergenic
AATTTGCTGATGGTGGTGAT+GGG 0.635503 3.1:-46261108 MS.gene84543:CDS
AAGATGCAATTTCAAGAGGA+CGG 0.635809 3.1:-46260370 MS.gene84543:CDS
TTTCCTGTTGTTCGACTGCG+CGG 0.636494 3.1:-46260959 MS.gene84543:CDS
TAAGAGGCAGGAATACTACA+AGG 0.645116 3.1:-46260738 MS.gene84543:CDS
CAAGGAGTATGTGTTGTCGG+AGG 0.654683 3.1:-46260552 MS.gene84543:CDS
CCCCTGAATTCATTACAATG+TGG 0.658546 3.1:+46260522 None:intergenic
CGTCAGCGGACAGTTGACGC+GGG 0.660169 3.1:-46261067 MS.gene84543:CDS
TTGTACATATAACTGGAACC+AGG 0.660817 3.1:+46261025 None:intergenic
CATCAACTATGTCTAGACCA+TGG 0.660852 3.1:+46260894 None:intergenic
CACATTGTAATGAATTCAGG+GGG 0.663594 3.1:-46260521 MS.gene84543:CDS
GCTCGGGGAGCACTACGACG+AGG 0.665801 3.1:+46260677 None:intergenic
AGTCGAACAACAGGAAATGG+TGG 0.675557 3.1:+46260965 None:intergenic
CCACATTGTAATGAATTCAG+GGG 0.677967 3.1:-46260522 MS.gene84543:CDS
ACAGGAGTGTTGCGACTGAG+GGG 0.684610 3.1:-46260617 MS.gene84543:CDS
AATGAGGTTTCAGATGCTCG+AGG 0.692222 3.1:-46260707 MS.gene84543:CDS
AAATGGCTTCAAAACGTCAG+CGG 0.695070 3.1:-46261081 MS.gene84543:CDS
GAGGTTTCAGATGCTCGAGG+CGG 0.695093 3.1:-46260704 MS.gene84543:CDS
GTACATATAACTGGAACCAG+GGG 0.705619 3.1:+46261027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATGATGAAGGAAAAGATT+CGG - chr3.1:46260876-46260895 MS.gene84543:CDS 25.0%
!! ATTACCATTTTCTGCTAATA+AGG - chr3.1:46260930-46260949 MS.gene84543:CDS 25.0%
!!! AAGCTTATTGACTTCAATTT+TGG - chr3.1:46260372-46260391 MS.gene84543:CDS 25.0%
!!! AATTTCAGTAAATTTGCTGA+TGG - chr3.1:46260406-46260425 MS.gene84543:CDS 25.0%
ATCCACATTGTAATGAATTC+AGG - chr3.1:46261000-46261019 MS.gene84543:CDS 30.0%
CTTCATCATATGATTTAGCT+CGG + chr3.1:46260867-46260886 None:intergenic 30.0%
TCATCCTTATTAGCAGAAAA+TGG + chr3.1:46260937-46260956 None:intergenic 30.0%
TCCACATTGTAATGAATTCA+GGG - chr3.1:46261001-46261020 MS.gene84543:CDS 30.0%
TGCTAATAAGGATGACATAA+TGG - chr3.1:46260942-46260961 MS.gene84543:CDS 30.0%
! GATGTTCTTTTTGTTCACAA+AGG - chr3.1:46260649-46260668 MS.gene84543:CDS 30.0%
! TTCATCATATGATTTAGCTC+GGG + chr3.1:46260866-46260885 None:intergenic 30.0%
! TTGAAGTTTTCAGAAGTAAG+AGG - chr3.1:46260770-46260789 MS.gene84543:CDS 30.0%
!! AGATGGATTGTACATATAAC+TGG + chr3.1:46260509-46260528 None:intergenic 30.0%
AAGATGCAATTTCAAGAGGA+CGG - chr3.1:46261154-46261173 MS.gene84543:CDS 35.0%
ACTTTGCTCTTCAAAGAAAC+AGG - chr3.1:46260725-46260744 MS.gene84543:CDS 35.0%
ATCAGTTTCAGTGCAATCAA+AGG + chr3.1:46260596-46260615 None:intergenic 35.0%
ATGGAAGATGCAATTTCAAG+AGG - chr3.1:46261150-46261169 MS.gene84543:CDS 35.0%
ATGTACAATCCATCTCCATA+TGG - chr3.1:46260514-46260533 MS.gene84543:CDS 35.0%
CACATTGTAATGAATTCAGG+GGG - chr3.1:46261003-46261022 MS.gene84543:CDS 35.0%
CCACATTGTAATGAATTCAG+GGG - chr3.1:46261002-46261021 MS.gene84543:CDS 35.0%
CGAGCTAAATCATATGATGA+AGG - chr3.1:46260865-46260884 MS.gene84543:CDS 35.0%
TCAGTTTCAGTGCAATCAAA+GGG + chr3.1:46260595-46260614 None:intergenic 35.0%
TGTACATATAACTGGAACCA+GGG + chr3.1:46260501-46260520 None:intergenic 35.0%
TTGTACATATAACTGGAACC+AGG + chr3.1:46260502-46260521 None:intergenic 35.0%
! GTTCTTTTTGTTCACAAAGG+TGG - chr3.1:46260652-46260671 MS.gene84543:CDS 35.0%
! TCATCATATGATTTAGCTCG+GGG + chr3.1:46260865-46260884 None:intergenic 35.0%
! TGACATAATGGAGTTCTTCA+AGG - chr3.1:46260954-46260973 MS.gene84543:CDS 35.0%
!! TTCAGTAAATTTGCTGATGG+TGG - chr3.1:46260409-46260428 MS.gene84543:CDS 35.0%
AAATGGCTTCAAAACGTCAG+CGG - chr3.1:46260443-46260462 MS.gene84543:CDS 40.0%
AGAGCAAAGTCAACTTGAAG+AGG + chr3.1:46260715-46260734 None:intergenic 40.0%
CAAAGAAACAGGCAGAACAT+AGG - chr3.1:46260736-46260755 MS.gene84543:CDS 40.0%
CAAAGGATAGAATGACGCTT+GGG - chr3.1:46261088-46261107 MS.gene84543:CDS 40.0%
CATCAACTATGTCTAGACCA+TGG + chr3.1:46260633-46260652 None:intergenic 40.0%
CCCCTGAATTCATTACAATG+TGG + chr3.1:46261005-46261024 None:intergenic 40.0%
CTGACATATCCATATGGAGA+TGG + chr3.1:46260526-46260545 None:intergenic 40.0%
GTACATATAACTGGAACCAG+GGG + chr3.1:46260500-46260519 None:intergenic 40.0%
TAAGAGGCAGGAATACTACA+AGG - chr3.1:46260786-46260805 MS.gene84543:CDS 40.0%
TCTTCATTCTATGATTCCCC+TGG - chr3.1:46260481-46260500 MS.gene84543:CDS 40.0%
TGATGATTCAAAAGCAGCCA+TGG - chr3.1:46261065-46261084 MS.gene84543:CDS 40.0%
TGATTGCACTGAAACTGATG+TGG - chr3.1:46260597-46260616 MS.gene84543:CDS 40.0%
! AGTTTTCAGAAGTAAGAGGC+AGG - chr3.1:46260774-46260793 MS.gene84543:CDS 40.0%
! CTTCAAGGAGTATGTGTTGT+CGG - chr3.1:46260969-46260988 MS.gene84543:CDS 40.0%
!! AAATTTGCTGATGGTGGTGA+TGG - chr3.1:46260415-46260434 MS.gene84543:CDS 40.0%
!! AATTTGCTGATGGTGGTGAT+GGG - chr3.1:46260416-46260435 MS.gene84543:CDS 40.0%
AATGAGGTTTCAGATGCTCG+AGG - chr3.1:46260817-46260836 MS.gene84543:CDS 45.0%
AGTCGAACAACAGGAAATGG+TGG + chr3.1:46260562-46260581 None:intergenic 45.0%
CAAAACACAGAAGCCTTCAC+CGG + chr3.1:46260686-46260705 None:intergenic 45.0%
CGTCATTCTATCCTTTGCCA+TGG + chr3.1:46261085-46261104 None:intergenic 45.0%
CTACAAGGCAGTTGCAAATG+AGG - chr3.1:46260801-46260820 MS.gene84543:CDS 45.0%
GCAAAGGATAGAATGACGCT+TGG - chr3.1:46261087-46261106 MS.gene84543:CDS 45.0%
GTCTAGACCATGGAAGAACT+CGG + chr3.1:46260623-46260642 None:intergenic 45.0%
TTCAAAAGCAGCCATGGCAA+AGG - chr3.1:46261071-46261090 MS.gene84543:CDS 45.0%
! CACAAAGGTGGCAAGTTTTC+CGG - chr3.1:46260664-46260683 MS.gene84543:CDS 45.0%
! GGTGAAGGCTTCTGTGTTTT+GGG - chr3.1:46260685-46260704 MS.gene84543:CDS 45.0%
! GGTTGGCTGACATATCCATA+TGG + chr3.1:46260532-46260551 None:intergenic 45.0%
AAAGATTCGGCTGAGCACAC+AGG - chr3.1:46260889-46260908 MS.gene84543:CDS 50.0%
AATTCAGGGGGAAGACCATC+TGG - chr3.1:46261015-46261034 MS.gene84543:CDS 50.0%
ATTCAGGGGGAAGACCATCT+GGG - chr3.1:46261016-46261035 MS.gene84543:CDS 50.0%
CCATCTCACCATGCGTTGAA+GGG + chr3.1:46261134-46261153 None:intergenic 50.0%
CGAACAACAGGAAATGGTGG+AGG + chr3.1:46260559-46260578 None:intergenic 50.0%
CGCAGTCGAACAACAGGAAA+TGG + chr3.1:46260565-46260584 None:intergenic 50.0%
GAGCTATTCCCTTCAACGCA+TGG - chr3.1:46261123-46261142 MS.gene84543:CDS 50.0%
TCCATCTCACCATGCGTTGA+AGG + chr3.1:46261135-46261154 None:intergenic 50.0%
TTTCCTGTTGTTCGACTGCG+CGG - chr3.1:46260565-46260584 MS.gene84543:CDS 50.0%
! ACAACAGGAAATGGTGGAGG+AGG + chr3.1:46260556-46260575 None:intergenic 50.0%
! CAAGGAGTATGTGTTGTCGG+AGG - chr3.1:46260972-46260991 MS.gene84543:CDS 50.0%
! CGGTGAAGGCTTCTGTGTTT+TGG - chr3.1:46260684-46260703 MS.gene84543:CDS 50.0%
ACCATCTGGGGAGGCTTATG+TGG - chr3.1:46261029-46261048 MS.gene84543:CDS 55.0%
CCCTTCAACGCATGGTGAGA+TGG - chr3.1:46261131-46261150 MS.gene84543:CDS 55.0%
GAGGTTTCAGATGCTCGAGG+CGG - chr3.1:46260820-46260839 MS.gene84543:CDS 55.0%
GATGTGGCCGAGTTCTTCCA+TGG - chr3.1:46260613-46260632 MS.gene84543:CDS 55.0%
TCCACATAAGCCTCCCCAGA+TGG + chr3.1:46261033-46261052 None:intergenic 55.0%
TTCAGGGGGAAGACCATCTG+GGG - chr3.1:46261017-46261036 MS.gene84543:CDS 55.0%
! ACAGGAAATGGTGGAGGAGG+AGG + chr3.1:46260553-46260572 None:intergenic 55.0%
! GAAATGGTGGAGGAGGAGGT+TGG + chr3.1:46260549-46260568 None:intergenic 55.0%
! GGTGGCAAGTTTTCCGGTGA+AGG - chr3.1:46260670-46260689 MS.gene84543:CDS 55.0%
!! ACACAGGAGTGTTGCGACTG+AGG - chr3.1:46260905-46260924 MS.gene84543:CDS 55.0%
!! ACAGGAGTGTTGCGACTGAG+GGG - chr3.1:46260907-46260926 MS.gene84543:CDS 55.0%
!! CACAGGAGTGTTGCGACTGA+GGG - chr3.1:46260906-46260925 MS.gene84543:CDS 55.0%
!! TGGTGGTGATGGGCGTGAAA+TGG - chr3.1:46260426-46260445 MS.gene84543:CDS 55.0%
ACGTCAGCGGACAGTTGACG+CGG - chr3.1:46260456-46260475 MS.gene84543:CDS 60.0%
AGACCGCGCAGTCGAACAAC+AGG + chr3.1:46260571-46260590 None:intergenic 60.0%
AGGGGGAAGACCATCTGGGG+AGG - chr3.1:46261020-46261039 MS.gene84543:CDS 65.0%
CGTCAGCGGACAGTTGACGC+GGG - chr3.1:46260457-46260476 MS.gene84543:CDS 65.0%
!! CTCGGGGAGCACTACGACGA+GGG + chr3.1:46260849-46260868 None:intergenic 65.0%
!! TCGGGGAGCACTACGACGAG+GGG + chr3.1:46260848-46260867 None:intergenic 65.0%
!! GCTCGGGGAGCACTACGACG+AGG + chr3.1:46260850-46260869 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 46260367 46261179 46260367 ID=MS.gene84543
chr3.1 mRNA 46260367 46261179 46260367 ID=MS.gene84543.t1;Parent=MS.gene84543
chr3.1 exon 46260367 46261179 46260367 ID=MS.gene84543.t1.exon1;Parent=MS.gene84543.t1
chr3.1 CDS 46260367 46261179 46260367 ID=cds.MS.gene84543.t1;Parent=MS.gene84543.t1
Gene Sequence

>MS.gene84543

ATGATAAGCTTATTGACTTCAATTTTGGTTATTGATGAAAATTTCAGTAAATTTGCTGATGGTGGTGATGGGCGTGAAATGGCTTCAAAACGTCAGCGGACAGTTGACGCGGGATCTTCATTCTATGATTCCCCTGGTTCCAGTTATATGTACAATCCATCTCCATATGGATATGTCAGCCAACCTCCTCCTCCACCATTTCCTGTTGTTCGACTGCGCGGTCTTCCCTTTGATTGCACTGAAACTGATGTGGCCGAGTTCTTCCATGGTCTAGACATAGTTGATGTTCTTTTTGTTCACAAAGGTGGCAAGTTTTCCGGTGAAGGCTTCTGTGTTTTGGGATATCCTCTTCAAGTTGACTTTGCTCTTCAAAGAAACAGGCAGAACATAGGTAGAAGATATGTTGAAGTTTTCAGAAGTAAGAGGCAGGAATACTACAAGGCAGTTGCAAATGAGGTTTCAGATGCTCGAGGCGGTTCCCCTCGTCGTAGTGCTCCCCGAGCTAAATCATATGATGAAGGAAAAGATTCGGCTGAGCACACAGGAGTGTTGCGACTGAGGGGATTACCATTTTCTGCTAATAAGGATGACATAATGGAGTTCTTCAAGGAGTATGTGTTGTCGGAGGATTCGATCCACATTGTAATGAATTCAGGGGGAAGACCATCTGGGGAGGCTTATGTGGAATTTGAAAATGCTGATGATTCAAAAGCAGCCATGGCAAAGGATAGAATGACGCTTGGGAGTCGCTATATAGAGCTATTCCCTTCAACGCATGGTGAGATGGAAGATGCAATTTCAAGAGGACGGTGA

Protein sequence

>MS.gene84543.t1

MISLLTSILVIDENFSKFADGGDGREMASKRQRTVDAGSSFYDSPGSSYMYNPSPYGYVSQPPPPPFPVVRLRGLPFDCTETDVAEFFHGLDIVDVLFVHKGGKFSGEGFCVLGYPLQVDFALQRNRQNIGRRYVEVFRSKRQEYYKAVANEVSDARGGSPRRSAPRAKSYDEGKDSAEHTGVLRLRGLPFSANKDDIMEFFKEYVLSEDSIHIVMNSGGRPSGEAYVEFENADDSKAAMAKDRMTLGSRYIELFPSTHGEMEDAISRGR