Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84729.t1 | XP_024629354.1 | 94.8 | 425 | 20 | 1 | 1 | 423 | 59 | 483 | 3.90E-228 | 800.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84729.t1 | F4IJ77 | 57.7 | 428 | 160 | 7 | 1 | 413 | 60 | 481 | 3.8e-131 | 469.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84729.t1 | A0A396GJ98 | 94.8 | 425 | 20 | 1 | 1 | 423 | 59 | 483 | 2.8e-228 | 800.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene84729 | 0.831789 | 1.37E-55 | -1.69E-46 |
| MS.gene054892 | MS.gene84729 | 0.818484 | 1.91E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene84729 | 0.82231 | 2.54E-53 | -1.69E-46 |
| MS.gene057622 | MS.gene84729 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene84729 | 0.832311 | 1.02E-55 | -1.69E-46 |
| MS.gene060518 | MS.gene84729 | 0.936279 | 2.24E-97 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84729.t1 | MTR_8g027200 | 89.176 | 425 | 18 | 2 | 1 | 423 | 59 | 457 | 0.0 | 757 |
| MS.gene84729.t1 | MTR_7g117950 | 63.855 | 415 | 126 | 9 | 1 | 414 | 61 | 452 | 0.0 | 524 |
| MS.gene84729.t1 | MTR_3g092060 | 52.506 | 419 | 175 | 9 | 4 | 412 | 66 | 470 | 3.93e-153 | 443 |
| MS.gene84729.t1 | MTR_2g061820 | 36.667 | 390 | 211 | 5 | 1 | 356 | 57 | 444 | 7.74e-87 | 274 |
| MS.gene84729.t1 | MTR_4g128260 | 34.048 | 420 | 224 | 8 | 1 | 371 | 59 | 474 | 1.68e-72 | 236 |
| MS.gene84729.t1 | MTR_8g076920 | 35.635 | 362 | 200 | 9 | 4 | 338 | 81 | 436 | 1.39e-67 | 223 |
| MS.gene84729.t1 | MTR_1g022250 | 34.264 | 394 | 206 | 10 | 7 | 357 | 69 | 452 | 2.75e-66 | 219 |
| MS.gene84729.t1 | MTR_2g030420 | 34.015 | 391 | 206 | 9 | 7 | 357 | 72 | 450 | 4.30e-66 | 218 |
| MS.gene84729.t1 | MTR_5g020980 | 35.393 | 356 | 199 | 5 | 34 | 360 | 100 | 453 | 1.10e-65 | 218 |
| MS.gene84729.t1 | MTR_2g076690 | 36.257 | 342 | 182 | 10 | 41 | 356 | 104 | 435 | 3.68e-65 | 216 |
| MS.gene84729.t1 | MTR_6g009720 | 34.211 | 380 | 195 | 8 | 7 | 335 | 71 | 446 | 5.45e-65 | 217 |
| MS.gene84729.t1 | MTR_2g076840 | 32.199 | 382 | 220 | 10 | 1 | 356 | 58 | 426 | 2.65e-64 | 219 |
| MS.gene84729.t1 | MTR_2g076840 | 41.532 | 248 | 130 | 6 | 122 | 357 | 460 | 704 | 1.85e-57 | 201 |
| MS.gene84729.t1 | MTR_6g009520 | 32.405 | 395 | 220 | 10 | 7 | 357 | 71 | 462 | 3.30e-64 | 215 |
| MS.gene84729.t1 | MTR_7g008040 | 34.400 | 375 | 196 | 9 | 29 | 356 | 89 | 460 | 3.59e-64 | 215 |
| MS.gene84729.t1 | MTR_4g107370 | 33.509 | 379 | 195 | 8 | 31 | 355 | 95 | 470 | 4.20e-64 | 214 |
| MS.gene84729.t1 | MTR_5g020990 | 33.420 | 386 | 201 | 10 | 17 | 356 | 95 | 470 | 2.02e-63 | 212 |
| MS.gene84729.t1 | MTR_6g009540 | 31.920 | 401 | 226 | 9 | 1 | 357 | 63 | 460 | 2.35e-63 | 213 |
| MS.gene84729.t1 | MTR_2g076970 | 36.913 | 298 | 167 | 6 | 70 | 356 | 141 | 428 | 2.47e-63 | 211 |
| MS.gene84729.t1 | MTR_6g009553 | 32.187 | 407 | 216 | 9 | 7 | 357 | 47 | 449 | 4.47e-63 | 211 |
| MS.gene84729.t1 | MTR_6g009770 | 32.099 | 405 | 217 | 9 | 7 | 357 | 48 | 448 | 4.52e-63 | 211 |
| MS.gene84729.t1 | MTR_2g076710 | 34.311 | 341 | 195 | 8 | 41 | 356 | 102 | 438 | 5.69e-63 | 209 |
| MS.gene84729.t1 | MTR_1g022245 | 33.618 | 351 | 181 | 7 | 34 | 335 | 91 | 438 | 1.62e-62 | 210 |
| MS.gene84729.t1 | MTR_2g076920 | 34.188 | 351 | 192 | 9 | 33 | 356 | 90 | 428 | 2.19e-62 | 209 |
| MS.gene84729.t1 | MTR_2g076900 | 31.332 | 383 | 229 | 9 | 1 | 356 | 58 | 433 | 3.04e-61 | 205 |
| MS.gene84729.t1 | MTR_6g042290 | 34.375 | 352 | 204 | 8 | 7 | 335 | 81 | 428 | 4.52e-60 | 203 |
| MS.gene84729.t1 | MTR_2g076990 | 29.684 | 411 | 236 | 11 | 1 | 382 | 1 | 387 | 7.97e-60 | 201 |
| MS.gene84729.t1 | MTR_3g103150 | 36.237 | 287 | 158 | 8 | 84 | 356 | 1 | 276 | 6.07e-58 | 191 |
| MS.gene84729.t1 | MTR_6g009510 | 31.989 | 372 | 197 | 9 | 1 | 356 | 75 | 406 | 3.43e-55 | 189 |
| MS.gene84729.t1 | MTR_6g009660 | 28.686 | 373 | 192 | 10 | 29 | 357 | 70 | 412 | 3.25e-44 | 160 |
| MS.gene84729.t1 | MTR_5g021020 | 32.177 | 317 | 170 | 13 | 56 | 356 | 118 | 405 | 3.83e-40 | 148 |
| MS.gene84729.t1 | MTR_5g020720 | 36.986 | 146 | 79 | 3 | 191 | 324 | 25 | 169 | 7.13e-21 | 90.1 |
| MS.gene84729.t1 | MTR_5g021000 | 38.889 | 108 | 61 | 4 | 251 | 356 | 1 | 105 | 7.62e-18 | 80.9 |
| MS.gene84729.t1 | MTR_1g011680 | 35.417 | 96 | 60 | 2 | 240 | 335 | 18 | 111 | 1.45e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84729.t1 | AT2G46620 | 57.666 | 437 | 164 | 7 | 1 | 423 | 60 | 489 | 1.93e-165 | 474 |
| MS.gene84729.t1 | AT4G25835 | 36.066 | 427 | 217 | 9 | 4 | 384 | 62 | 478 | 4.48e-77 | 248 |
| MS.gene84729.t1 | AT5G57480 | 35.426 | 446 | 217 | 10 | 4 | 399 | 62 | 486 | 8.88e-76 | 245 |
| MS.gene84729.t1 | AT5G17760 | 41.776 | 304 | 147 | 8 | 71 | 356 | 163 | 454 | 2.64e-69 | 228 |
| MS.gene84729.t1 | AT3G28580 | 36.182 | 351 | 180 | 7 | 30 | 338 | 91 | 439 | 4.82e-69 | 227 |
| MS.gene84729.t1 | AT5G40010 | 35.501 | 369 | 188 | 9 | 26 | 348 | 90 | 454 | 6.92e-68 | 224 |
| MS.gene84729.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 62 | 459 | 2.56e-67 | 223 |
| MS.gene84729.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 64 | 461 | 2.73e-67 | 223 |
| MS.gene84729.t1 | AT3G28580 | 36.585 | 328 | 188 | 6 | 30 | 338 | 91 | 417 | 1.98e-66 | 219 |
| MS.gene84729.t1 | AT2G18193 | 38.554 | 332 | 177 | 8 | 34 | 343 | 101 | 427 | 2.50e-66 | 220 |
| MS.gene84729.t1 | AT3G50930 | 35.933 | 359 | 189 | 10 | 34 | 357 | 148 | 500 | 2.84e-66 | 221 |
| MS.gene84729.t1 | AT3G28610 | 37.425 | 334 | 175 | 10 | 37 | 343 | 106 | 432 | 1.59e-65 | 217 |
| MS.gene84729.t1 | AT3G28600 | 36.888 | 347 | 185 | 11 | 37 | 356 | 107 | 446 | 7.35e-65 | 215 |
| MS.gene84729.t1 | AT5G17750 | 36.364 | 341 | 179 | 7 | 13 | 322 | 57 | 390 | 1.80e-64 | 212 |
| MS.gene84729.t1 | AT3G50940 | 35.455 | 330 | 181 | 7 | 34 | 338 | 102 | 424 | 2.35e-64 | 213 |
| MS.gene84729.t1 | AT2G18190 | 36.474 | 329 | 181 | 7 | 34 | 338 | 102 | 426 | 1.49e-63 | 213 |
| MS.gene84729.t1 | AT5G17730 | 37.374 | 297 | 165 | 7 | 73 | 356 | 155 | 443 | 5.49e-63 | 210 |
| MS.gene84729.t1 | AT5G17740 | 31.765 | 425 | 230 | 13 | 3 | 390 | 71 | 472 | 5.63e-63 | 212 |
| MS.gene84729.t1 | AT3G28510 | 33.333 | 369 | 196 | 8 | 33 | 356 | 95 | 458 | 3.35e-62 | 210 |
| MS.gene84729.t1 | AT3G28540 | 34.302 | 344 | 180 | 8 | 33 | 335 | 96 | 434 | 4.35e-62 | 209 |
| MS.gene84729.t1 | AT3G28540 | 34.302 | 344 | 180 | 8 | 33 | 335 | 96 | 434 | 4.95e-62 | 209 |
| MS.gene84729.t1 | AT5G40000 | 35.652 | 345 | 191 | 11 | 37 | 356 | 111 | 449 | 3.19e-61 | 206 |
| MS.gene84729.t1 | AT4G05380 | 36.905 | 336 | 178 | 7 | 47 | 356 | 120 | 447 | 3.04e-60 | 203 |
| MS.gene84729.t1 | AT3G28600 | 39.785 | 279 | 141 | 7 | 103 | 356 | 131 | 407 | 9.91e-60 | 201 |
| MS.gene84729.t1 | AT3G28570 | 33.239 | 352 | 212 | 9 | 22 | 356 | 88 | 433 | 1.85e-58 | 198 |
| MS.gene84729.t1 | AT3G28570 | 33.239 | 352 | 212 | 9 | 22 | 356 | 95 | 440 | 2.39e-58 | 198 |
| MS.gene84729.t1 | AT1G43910 | 34.259 | 324 | 179 | 7 | 47 | 343 | 119 | 435 | 4.31e-53 | 184 |
| MS.gene84729.t1 | AT3G28520 | 33.236 | 343 | 187 | 8 | 33 | 338 | 85 | 422 | 8.04e-53 | 184 |
| MS.gene84729.t1 | AT5G17760 | 48.428 | 159 | 75 | 2 | 71 | 229 | 163 | 314 | 1.90e-40 | 147 |
| MS.gene84729.t1 | AT3G29800 | 27.964 | 329 | 208 | 10 | 27 | 342 | 55 | 367 | 2.40e-36 | 138 |
| MS.gene84729.t1 | AT4G05340 | 47.826 | 69 | 35 | 1 | 242 | 310 | 27 | 94 | 2.10e-13 | 66.2 |
Find 97 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATGGTTTGTTTAGATTA+AGG | 0.215457 | 8.3:-67220544 | MS.gene84729:CDS |
| ACGGATTTGAGGAAGATTTA+TGG | 0.228137 | 8.3:-67220560 | MS.gene84729:CDS |
| TCGGTTTATAACATCGGATT+TGG | 0.271342 | 8.3:-67221053 | MS.gene84729:CDS |
| TTCTCGGCGCCACGGTTTAT+TGG | 0.279842 | 8.3:-67221588 | MS.gene84729:CDS |
| GAATAGGTCGGGTAATTTAT+TGG | 0.293961 | 8.3:-67220510 | MS.gene84729:CDS |
| GCTGAGTATTCTGCGTTTAT+CGG | 0.300204 | 8.3:+67221507 | None:intergenic |
| GGGGTGGTGGAAGGAGGTGG+AGG | 0.315568 | 8.3:-67221393 | MS.gene84729:CDS |
| ACAAATGCACCTGTTTGATT+TGG | 0.324681 | 8.3:+67221547 | None:intergenic |
| AACATTTGAAACGATGTTGA+TGG | 0.338402 | 8.3:-67221347 | MS.gene84729:CDS |
| AATGTTGATGGATGATTAAA+CGG | 0.346660 | 8.3:+67221364 | None:intergenic |
| TAAGGAATGTTAAGAGGAAT+AGG | 0.348823 | 8.3:-67220526 | MS.gene84729:CDS |
| CCGGCAACGGTTACGGCGGC+GGG | 0.352366 | 8.3:-67221025 | MS.gene84729:CDS |
| AAACGGAGCTTCTTGTTATA+CGG | 0.355478 | 8.3:-67221238 | MS.gene84729:CDS |
| ATAAAAGAATCGGTCGTGTT+TGG | 0.378794 | 8.3:-67221261 | MS.gene84729:CDS |
| TGCACCTGTTTGATTTGGTT+CGG | 0.381073 | 8.3:+67221552 | None:intergenic |
| GACTTCACCAATCTCATTAC+CGG | 0.382732 | 8.3:-67221670 | MS.gene84729:CDS |
| GAAAATCATTATTCTTTCTT+CGG | 0.384048 | 8.3:+67220961 | None:intergenic |
| ATTATTTCCGGTAATGAGAT+TGG | 0.397632 | 8.3:+67221663 | None:intergenic |
| CGGGCTATTAAATCGGTAAT+CGG | 0.398376 | 8.3:-67220704 | MS.gene84729:CDS |
| CCCGCCGCCGTAACCGTTGC+CGG | 0.398549 | 8.3:+67221025 | None:intergenic |
| TCCGGCAACGGTTACGGCGG+CGG | 0.415110 | 8.3:-67221026 | MS.gene84729:CDS |
| CGACCAGGTCGGAGAAAATT+CGG | 0.415681 | 8.3:+67220911 | None:intergenic |
| AACATCGTTTCAAATGTTGA+TGG | 0.416178 | 8.3:+67221352 | None:intergenic |
| TTCGTTAATATCACCGGCGG+TGG | 0.419095 | 8.3:-67221415 | MS.gene84729:CDS |
| GGTAATCGGGGCATTGCAAA+TGG | 0.420117 | 8.3:-67220690 | MS.gene84729:CDS |
| GGAATCTCCGGCAACGGTTA+CGG | 0.421241 | 8.3:-67221032 | MS.gene84729:CDS |
| GTTAATATCACCGGCGGTGG+GGG | 0.422663 | 8.3:-67221412 | MS.gene84729:CDS |
| TCAACAACGATAATAGATTT+CGG | 0.429950 | 8.3:+67221085 | None:intergenic |
| TTCTTGTTATACGGTGAATC+CGG | 0.433520 | 8.3:-67221229 | MS.gene84729:CDS |
| GGGCTATTAAATCGGTAATC+GGG | 0.437429 | 8.3:-67220703 | MS.gene84729:CDS |
| AACATCGGATTTGGAATCTC+CGG | 0.444407 | 8.3:-67221044 | MS.gene84729:CDS |
| TTAGATTAAGGAATGTTAAG+AGG | 0.444604 | 8.3:-67220532 | MS.gene84729:CDS |
| GGTGTTCTTTGTGGTGGTGA+TGG | 0.454004 | 8.3:-67220590 | MS.gene84729:CDS |
| AGTTTGAGTCCGGCGGAGAT+AGG | 0.458254 | 8.3:-67220761 | MS.gene84729:CDS |
| CGGAAAATCTCCTCCACCGC+CGG | 0.462392 | 8.3:+67220794 | None:intergenic |
| GAATCGGTCGTGTTTGGAAA+CGG | 0.462731 | 8.3:-67221255 | MS.gene84729:CDS |
| ATCATCGAAGACAGGTTTCT+CGG | 0.463592 | 8.3:-67221604 | MS.gene84729:CDS |
| GACGGTGATGGAAAAGGGTG+TGG | 0.465085 | 8.3:-67220668 | MS.gene84729:CDS |
| AAGAATTATTATAAAAGAAT+CGG | 0.466313 | 8.3:-67221271 | MS.gene84729:CDS |
| GCCGAGTCGGGCTATTAAAT+CGG | 0.467127 | 8.3:-67220711 | MS.gene84729:CDS |
| GACTCAAACTCGCTCCCTGC+CGG | 0.469388 | 8.3:+67220774 | None:intergenic |
| TCGTTAATATCACCGGCGGT+GGG | 0.469999 | 8.3:-67221414 | MS.gene84729:CDS |
| TATGATCGTACGGCGGATAG+AGG | 0.472611 | 8.3:-67220642 | MS.gene84729:CDS |
| ACTTTACGATAAAACATGTT+CGG | 0.478150 | 8.3:+67221733 | None:intergenic |
| ATCTAGCTTTGTTGCAGCCA+TGG | 0.479264 | 8.3:-67221197 | MS.gene84729:CDS |
| GAATCGGAACTCGCCGAGTC+GGG | 0.479389 | 8.3:-67220723 | MS.gene84729:CDS |
| AATTTATTGGTCAATGATGA+AGG | 0.489531 | 8.3:-67220497 | MS.gene84729:CDS |
| GCTTTGGCGAATAGTTACTT+AGG | 0.495433 | 8.3:-67220839 | MS.gene84729:CDS |
| ATAAAACATGTTCGGTTGCA+TGG | 0.503776 | 8.3:+67221741 | None:intergenic |
| CGTTAATATCACCGGCGGTG+GGG | 0.505377 | 8.3:-67221413 | MS.gene84729:CDS |
| AAAACCGAACCAAATCAAAC+AGG | 0.507238 | 8.3:-67221556 | MS.gene84729:CDS |
| ACGGTGATGGAAAAGGGTGT+GGG | 0.516936 | 8.3:-67220667 | MS.gene84729:CDS |
| GGCGGGGATTCAGAATTTCA+TGG | 0.525375 | 8.3:-67221008 | MS.gene84729:CDS |
| ATATGAACGTCCACACGACC+AGG | 0.525421 | 8.3:+67220896 | None:intergenic |
| GCAGGGAGCGAGTTTGAGTC+CGG | 0.529877 | 8.3:-67220771 | MS.gene84729:CDS |
| CGTTTGTTCGTTAATATCAC+CGG | 0.539498 | 8.3:-67221421 | MS.gene84729:CDS |
| GAAATCTATTATCGTTGTTG+AGG | 0.540126 | 8.3:-67221083 | MS.gene84729:CDS |
| GAATGTTAAGAGGAATAGGT+CGG | 0.544625 | 8.3:-67220522 | MS.gene84729:CDS |
| GTGAGTTGATGATTGCGAAT+CGG | 0.552462 | 8.3:-67220739 | MS.gene84729:CDS |
| CGAATCGGAACTCGCCGAGT+CGG | 0.559326 | 8.3:-67220724 | MS.gene84729:CDS |
| CCGATCAAATCATCGAAGAC+AGG | 0.562107 | 8.3:-67221612 | MS.gene84729:CDS |
| GTATTCTATTTCAGCGGACA+TGG | 0.562971 | 8.3:+67221462 | None:intergenic |
| CGGTGATGGAAAAGGGTGTG+GGG | 0.563898 | 8.3:-67220666 | MS.gene84729:CDS |
| AATGTTAAGAGGAATAGGTC+GGG | 0.568612 | 8.3:-67220521 | MS.gene84729:CDS |
| CCTGTCTTCGATGATTTGAT+CGG | 0.569361 | 8.3:+67221612 | None:intergenic |
| CAAATGGACGGTGATGGAAA+AGG | 0.573028 | 8.3:-67220674 | MS.gene84729:CDS |
| GCCGTTGTATTCTATTTCAG+CGG | 0.577178 | 8.3:+67221456 | None:intergenic |
| ATCAACTCACCTATCTCCGC+CGG | 0.583450 | 8.3:+67220752 | None:intergenic |
| TTATACGGTGAATCCGGTAC+CGG | 0.585271 | 8.3:-67221223 | MS.gene84729:CDS |
| AGACAGGTTTCTCGGCGCCA+CGG | 0.586250 | 8.3:-67221596 | MS.gene84729:CDS |
| TCTCGATCGGTTTATAACAT+CGG | 0.589482 | 8.3:-67221059 | MS.gene84729:CDS |
| AGTAACCAAGGTGTTCTTTG+TGG | 0.595481 | 8.3:-67220599 | MS.gene84729:CDS |
| GGATTTGGAATCTCCGGCAA+CGG | 0.595822 | 8.3:-67221038 | MS.gene84729:CDS |
| GCATTGCAAATGGACGGTGA+TGG | 0.605343 | 8.3:-67220680 | MS.gene84729:CDS |
| GGCTATTAAATCGGTAATCG+GGG | 0.605421 | 8.3:-67220702 | MS.gene84729:CDS |
| ATCGGGGCATTGCAAATGGA+CGG | 0.606342 | 8.3:-67220686 | MS.gene84729:CDS |
| AAGTCGGAATCTTCAAGAGA+AGG | 0.608105 | 8.3:+67221688 | None:intergenic |
| AAATGGACGGTGATGGAAAA+GGG | 0.611545 | 8.3:-67220673 | MS.gene84729:CDS |
| ATCTCCGGCAACGGTTACGG+CGG | 0.614797 | 8.3:-67221029 | MS.gene84729:CDS |
| GGTAATGAGATTGGTGAAGT+CGG | 0.621262 | 8.3:+67221672 | None:intergenic |
| GGGATTCAGAATTTCATGGA+CGG | 0.622282 | 8.3:-67221004 | MS.gene84729:CDS |
| TTTATAGAACCAATAAACCG+TGG | 0.631666 | 8.3:+67221579 | None:intergenic |
| ACCGATTTAATAGCCCGACT+CGG | 0.633107 | 8.3:+67220710 | None:intergenic |
| GAACGTCCACACGACCAGGT+CGG | 0.633799 | 8.3:+67220900 | None:intergenic |
| AACCAAGGTGTTCTTTGTGG+TGG | 0.636999 | 8.3:-67220596 | MS.gene84729:CDS |
| CGGCAACGGTTACGGCGGCG+GGG | 0.642529 | 8.3:-67221024 | MS.gene84729:CDS |
| GGTGTGGGGATATGATCGTA+CGG | 0.644792 | 8.3:-67220652 | MS.gene84729:CDS |
| TCGTTGTTGAGGATCTCGAT+CGG | 0.655691 | 8.3:-67221072 | MS.gene84729:CDS |
| ATAACAAAGAAAATTCGCCA+TGG | 0.656962 | 8.3:+67221180 | None:intergenic |
| GAATAGTTACTTAGGTGTGA+AGG | 0.666896 | 8.3:-67220831 | MS.gene84729:CDS |
| TTGTTCGTTAATATCACCGG+CGG | 0.670649 | 8.3:-67221418 | MS.gene84729:CDS |
| CACCACCACAAAGAACACCT+TGG | 0.671779 | 8.3:+67220594 | None:intergenic |
| GCGGATAGAGGATGATGACG+TGG | 0.692126 | 8.3:-67220630 | MS.gene84729:CDS |
| GTGGAAGATGAAAGTAACCA+AGG | 0.702124 | 8.3:-67220611 | MS.gene84729:CDS |
| GGGAGCGAGTTTGAGTCCGG+CGG | 0.703907 | 8.3:-67220768 | MS.gene84729:CDS |
| TCCGCTGAAATAGAATACAA+CGG | 0.706310 | 8.3:-67221457 | MS.gene84729:CDS |
| GTGGGGATATGATCGTACGG+CGG | 0.779328 | 8.3:-67220649 | MS.gene84729:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAATTATTATAAAAGAAT+CGG | - | chr8.3:67220958-67220977 | MS.gene84729:CDS | 10.0% |
| !! | GAAAATCATTATTCTTTCTT+CGG | + | chr8.3:67221271-67221290 | None:intergenic | 20.0% |
| !!! | TTTATGGTTTGTTTAGATTA+AGG | - | chr8.3:67221685-67221704 | MS.gene84729:CDS | 20.0% |
| !!! | TTTTTCATCTTTTAAAGCTT+TGG | - | chr8.3:67221374-67221393 | MS.gene84729:CDS | 20.0% |
| ! | AATTTATTGGTCAATGATGA+AGG | - | chr8.3:67221732-67221751 | MS.gene84729:CDS | 25.0% |
| ! | ACTTTACGATAAAACATGTT+CGG | + | chr8.3:67220499-67220518 | None:intergenic | 25.0% |
| ! | TCAACAACGATAATAGATTT+CGG | + | chr8.3:67221147-67221166 | None:intergenic | 25.0% |
| ! | TTAGATTAAGGAATGTTAAG+AGG | - | chr8.3:67221697-67221716 | MS.gene84729:CDS | 25.0% |
| !! | AATGTTGATGGATGATTAAA+CGG | + | chr8.3:67220868-67220887 | None:intergenic | 25.0% |
| AAAGATGAAAAATCACACAC+CGG | + | chr8.3:67221366-67221385 | None:intergenic | 30.0% | |
| AACATCGTTTCAAATGTTGA+TGG | + | chr8.3:67220880-67220899 | None:intergenic | 30.0% | |
| ATAACAAAGAAAATTCGCCA+TGG | + | chr8.3:67221052-67221071 | None:intergenic | 30.0% | |
| ATTATTTCCGGTAATGAGAT+TGG | + | chr8.3:67220569-67220588 | None:intergenic | 30.0% | |
| GAAATCTATTATCGTTGTTG+AGG | - | chr8.3:67221146-67221165 | MS.gene84729:CDS | 30.0% | |
| TAAGGAATGTTAAGAGGAAT+AGG | - | chr8.3:67221703-67221722 | MS.gene84729:CDS | 30.0% | |
| TTTATAGAACCAATAAACCG+TGG | + | chr8.3:67220653-67220672 | None:intergenic | 30.0% | |
| ! | AACATTTGAAACGATGTTGA+TGG | - | chr8.3:67220882-67220901 | MS.gene84729:CDS | 30.0% |
| !!! | GATGTCGTTTTGATTATTTC+CGG | + | chr8.3:67220581-67220600 | None:intergenic | 30.0% |
| AAAACCGAACCAAATCAAAC+AGG | - | chr8.3:67220673-67220692 | MS.gene84729:CDS | 35.0% | |
| AATGTTAAGAGGAATAGGTC+GGG | - | chr8.3:67221708-67221727 | MS.gene84729:CDS | 35.0% | |
| ACAAATGCACCTGTTTGATT+TGG | + | chr8.3:67220685-67220704 | None:intergenic | 35.0% | |
| ACGGATTTGAGGAAGATTTA+TGG | - | chr8.3:67221669-67221688 | MS.gene84729:CDS | 35.0% | |
| ATAAAAGAATCGGTCGTGTT+TGG | - | chr8.3:67220968-67220987 | MS.gene84729:CDS | 35.0% | |
| CGTTTGTTCGTTAATATCAC+CGG | - | chr8.3:67220808-67220827 | MS.gene84729:CDS | 35.0% | |
| GAATAGGTCGGGTAATTTAT+TGG | - | chr8.3:67221719-67221738 | MS.gene84729:CDS | 35.0% | |
| GAATAGTTACTTAGGTGTGA+AGG | - | chr8.3:67221398-67221417 | MS.gene84729:CDS | 35.0% | |
| GAATGTTAAGAGGAATAGGT+CGG | - | chr8.3:67221707-67221726 | MS.gene84729:CDS | 35.0% | |
| TCCGCTGAAATAGAATACAA+CGG | - | chr8.3:67220772-67220791 | MS.gene84729:CDS | 35.0% | |
| TCGGTTTATAACATCGGATT+TGG | - | chr8.3:67221176-67221195 | MS.gene84729:CDS | 35.0% | |
| ! | AAACGGAGCTTCTTGTTATA+CGG | - | chr8.3:67220991-67221010 | MS.gene84729:CDS | 35.0% |
| ! | ATGGTTTTTCTACGGATTTG+AGG | - | chr8.3:67221658-67221677 | MS.gene84729:CDS | 35.0% |
| ! | GGACGTTCATATTCATTTTC+CGG | - | chr8.3:67221344-67221363 | MS.gene84729:CDS | 35.0% |
| ! | TCTCGATCGGTTTATAACAT+CGG | - | chr8.3:67221170-67221189 | MS.gene84729:CDS | 35.0% |
| ! | TGCAACAAAGCTAGATTTTC+CGG | + | chr8.3:67221028-67221047 | None:intergenic | 35.0% |
| ! | TTCTTGTTATACGGTGAATC+CGG | - | chr8.3:67221000-67221019 | MS.gene84729:CDS | 35.0% |
| AAGTCGGAATCTTCAAGAGA+AGG | + | chr8.3:67220544-67220563 | None:intergenic | 40.0% | |
| ATCATCGAAGACAGGTTTCT+CGG | - | chr8.3:67220625-67220644 | MS.gene84729:CDS | 40.0% | |
| CCTGTCTTCGATGATTTGAT+CGG | + | chr8.3:67220620-67220639 | None:intergenic | 40.0% | |
| CGGGCTATTAAATCGGTAAT+CGG | - | chr8.3:67221525-67221544 | MS.gene84729:CDS | 40.0% | |
| GACTTCACCAATCTCATTAC+CGG | - | chr8.3:67220559-67220578 | MS.gene84729:CDS | 40.0% | |
| GCCGTTGTATTCTATTTCAG+CGG | + | chr8.3:67220776-67220795 | None:intergenic | 40.0% | |
| GCTGAGTATTCTGCGTTTAT+CGG | + | chr8.3:67220725-67220744 | None:intergenic | 40.0% | |
| GCTTTGGCGAATAGTTACTT+AGG | - | chr8.3:67221390-67221409 | MS.gene84729:CDS | 40.0% | |
| GGCTATTAAATCGGTAATCG+GGG | - | chr8.3:67221527-67221546 | MS.gene84729:CDS | 40.0% | |
| GGGATTCAGAATTTCATGGA+CGG | - | chr8.3:67221225-67221244 | MS.gene84729:CDS | 40.0% | |
| GGGCTATTAAATCGGTAATC+GGG | - | chr8.3:67221526-67221545 | MS.gene84729:CDS | 40.0% | |
| GGTAATGAGATTGGTGAAGT+CGG | + | chr8.3:67220560-67220579 | None:intergenic | 40.0% | |
| GTATTCTATTTCAGCGGACA+TGG | + | chr8.3:67220770-67220789 | None:intergenic | 40.0% | |
| GTGGAAGATGAAAGTAACCA+AGG | - | chr8.3:67221618-67221637 | MS.gene84729:CDS | 40.0% | |
| TTGTTCGTTAATATCACCGG+CGG | - | chr8.3:67220811-67220830 | MS.gene84729:CDS | 40.0% | |
| ! | AAAGCTAGATTTTCCGGTAC+CGG | + | chr8.3:67221022-67221041 | None:intergenic | 40.0% |
| ! | AACATCGGATTTGGAATCTC+CGG | - | chr8.3:67221185-67221204 | MS.gene84729:CDS | 40.0% |
| ! | AGTAACCAAGGTGTTCTTTG+TGG | - | chr8.3:67221630-67221649 | MS.gene84729:CDS | 40.0% |
| !! | AAATGGACGGTGATGGAAAA+GGG | - | chr8.3:67221556-67221575 | MS.gene84729:CDS | 40.0% |
| !! | GAAGGAACACAAGCTTTTTC+CGG | - | chr8.3:67221416-67221435 | MS.gene84729:CDS | 40.0% |
| !! | GTGAGTTGATGATTGCGAAT+CGG | - | chr8.3:67221490-67221509 | MS.gene84729:CDS | 40.0% |
| !! | TGCACCTGTTTGATTTGGTT+CGG | + | chr8.3:67220680-67220699 | None:intergenic | 40.0% |
| !!! | TGGTGGTGATGGTTTTTCTA+CGG | - | chr8.3:67221650-67221669 | MS.gene84729:CDS | 40.0% |
| CCGATCAAATCATCGAAGAC+AGG | - | chr8.3:67220617-67220636 | MS.gene84729:CDS | 45.0% | |
| GAATCGGTCGTGTTTGGAAA+CGG | - | chr8.3:67220974-67220993 | MS.gene84729:CDS | 45.0% | |
| TCGTTGTTGAGGATCTCGAT+CGG | - | chr8.3:67221157-67221176 | MS.gene84729:CDS | 45.0% | |
| TTATACGGTGAATCCGGTAC+CGG | - | chr8.3:67221006-67221025 | MS.gene84729:CDS | 45.0% | |
| ! | AACCAAGGTGTTCTTTGTGG+TGG | - | chr8.3:67221633-67221652 | MS.gene84729:CDS | 45.0% |
| ! | ACCGATTTAATAGCCCGACT+CGG | + | chr8.3:67221522-67221541 | None:intergenic | 45.0% |
| !! | ATCTAGCTTTGTTGCAGCCA+TGG | - | chr8.3:67221032-67221051 | MS.gene84729:CDS | 45.0% |
| !! | CAAATGGACGGTGATGGAAA+AGG | - | chr8.3:67221555-67221574 | MS.gene84729:CDS | 45.0% |
| ATATGAACGTCCACACGACC+AGG | + | chr8.3:67221336-67221355 | None:intergenic | 50.0% | |
| ATCAACTCACCTATCTCCGC+CGG | + | chr8.3:67221480-67221499 | None:intergenic | 50.0% | |
| CACCACCACAAAGAACACCT+TGG | + | chr8.3:67221638-67221657 | None:intergenic | 50.0% | |
| CGACCAGGTCGGAGAAAATT+CGG | + | chr8.3:67221321-67221340 | None:intergenic | 50.0% | |
| GCATTGCAAATGGACGGTGA+TGG | - | chr8.3:67221549-67221568 | MS.gene84729:CDS | 50.0% | |
| GCCGAGTCGGGCTATTAAAT+CGG | - | chr8.3:67221518-67221537 | MS.gene84729:CDS | 50.0% | |
| GGCGGGGATTCAGAATTTCA+TGG | - | chr8.3:67221221-67221240 | MS.gene84729:CDS | 50.0% | |
| GGTGTGGGGATATGATCGTA+CGG | - | chr8.3:67221577-67221596 | MS.gene84729:CDS | 50.0% | |
| TATGATCGTACGGCGGATAG+AGG | - | chr8.3:67221587-67221606 | MS.gene84729:CDS | 50.0% | |
| TCGTTAATATCACCGGCGGT+GGG | - | chr8.3:67220815-67220834 | MS.gene84729:CDS | 50.0% | |
| TTCGTTAATATCACCGGCGG+TGG | - | chr8.3:67220814-67220833 | MS.gene84729:CDS | 50.0% | |
| ! | GATCCGAATTTTCTCCGACC+TGG | - | chr8.3:67221315-67221334 | MS.gene84729:CDS | 50.0% |
| ! | GGATTTGGAATCTCCGGCAA+CGG | - | chr8.3:67221191-67221210 | MS.gene84729:CDS | 50.0% |
| ! | GGTGTTCTTTGTGGTGGTGA+TGG | - | chr8.3:67221639-67221658 | MS.gene84729:CDS | 50.0% |
| !! | ACGGTGATGGAAAAGGGTGT+GGG | - | chr8.3:67221562-67221581 | MS.gene84729:CDS | 50.0% |
| !! | ATCGGGGCATTGCAAATGGA+CGG | - | chr8.3:67221543-67221562 | MS.gene84729:CDS | 50.0% |
| !! | GGAACACAAGCTTTTTCCGG+CGG | - | chr8.3:67221419-67221438 | MS.gene84729:CDS | 50.0% |
| !! | GGTAATCGGGGCATTGCAAA+TGG | - | chr8.3:67221539-67221558 | MS.gene84729:CDS | 50.0% |
| AGTTTGAGTCCGGCGGAGAT+AGG | - | chr8.3:67221468-67221487 | MS.gene84729:CDS | 55.0% | |
| CGTTAATATCACCGGCGGTG+GGG | - | chr8.3:67220816-67220835 | MS.gene84729:CDS | 55.0% | |
| GGAATCTCCGGCAACGGTTA+CGG | - | chr8.3:67221197-67221216 | MS.gene84729:CDS | 55.0% | |
| GTGGGGATATGATCGTACGG+CGG | - | chr8.3:67221580-67221599 | MS.gene84729:CDS | 55.0% | |
| GTTAATATCACCGGCGGTGG+GGG | - | chr8.3:67220817-67220836 | MS.gene84729:CDS | 55.0% | |
| TTCTCGGCGCCACGGTTTAT+TGG | - | chr8.3:67220641-67220660 | MS.gene84729:CDS | 55.0% | |
| ! | GCGGATAGAGGATGATGACG+TGG | - | chr8.3:67221599-67221618 | MS.gene84729:CDS | 55.0% |
| ! | GTGGAGGAGATTTTCCGGCA+GGG | - | chr8.3:67221441-67221460 | MS.gene84729:CDS | 55.0% |
| ! | TTTTCTCCGACCTGGTCGTG+TGG | - | chr8.3:67221323-67221342 | MS.gene84729:CDS | 55.0% |
| !! | ACACAAGCTTTTTCCGGCGG+TGG | - | chr8.3:67221422-67221441 | MS.gene84729:CDS | 55.0% |
| !! | CGGTGATGGAAAAGGGTGTG+GGG | - | chr8.3:67221563-67221582 | MS.gene84729:CDS | 55.0% |
| !! | GACGGTGATGGAAAAGGGTG+TGG | - | chr8.3:67221561-67221580 | MS.gene84729:CDS | 55.0% |
| AGACAGGTTTCTCGGCGCCA+CGG | - | chr8.3:67220633-67220652 | MS.gene84729:CDS | 60.0% | |
| ATCTCCGGCAACGGTTACGG+CGG | - | chr8.3:67221200-67221219 | MS.gene84729:CDS | 60.0% | |
| CGAATCGGAACTCGCCGAGT+CGG | - | chr8.3:67221505-67221524 | MS.gene84729:CDS | 60.0% | |
| CGGAAAATCTCCTCCACCGC+CGG | + | chr8.3:67221438-67221457 | None:intergenic | 60.0% | |
| GAACGTCCACACGACCAGGT+CGG | + | chr8.3:67221332-67221351 | None:intergenic | 60.0% | |
| GAATCGGAACTCGCCGAGTC+GGG | - | chr8.3:67221506-67221525 | MS.gene84729:CDS | 60.0% | |
| GACTCAAACTCGCTCCCTGC+CGG | + | chr8.3:67221458-67221477 | None:intergenic | 60.0% | |
| GCAGGGAGCGAGTTTGAGTC+CGG | - | chr8.3:67221458-67221477 | MS.gene84729:CDS | 60.0% | |
| ! | CGGCGGTGGAGGAGATTTTC+CGG | - | chr8.3:67221436-67221455 | MS.gene84729:CDS | 60.0% |
| ! | GGTGGAGGAGATTTTCCGGC+AGG | - | chr8.3:67221440-67221459 | MS.gene84729:CDS | 60.0% |
| !! | CAAGCTTTTTCCGGCGGTGG+AGG | - | chr8.3:67221425-67221444 | MS.gene84729:CDS | 60.0% |
| AATATCACCGGCGGTGGGGG+TGG | - | chr8.3:67220820-67220839 | MS.gene84729:CDS | 65.0% | |
| GGGAGCGAGTTTGAGTCCGG+CGG | - | chr8.3:67221461-67221480 | MS.gene84729:CDS | 65.0% | |
| TCCGGCAACGGTTACGGCGG+CGG | - | chr8.3:67221203-67221222 | MS.gene84729:CDS | 70.0% | |
| !! | GGGGTGGTGGAAGGAGGTGG+AGG | - | chr8.3:67220836-67220855 | MS.gene84729:CDS | 70.0% |
| !! | GTGGGGGTGGTGGAAGGAGG+TGG | - | chr8.3:67220833-67220852 | MS.gene84729:CDS | 70.0% |
| ATCACCGGCGGTGGGGGTGG+TGG | - | chr8.3:67220823-67220842 | MS.gene84729:CDS | 75.0% | |
| CCCGCCGCCGTAACCGTTGC+CGG | + | chr8.3:67221207-67221226 | None:intergenic | 75.0% | |
| CCGGCAACGGTTACGGCGGC+GGG | - | chr8.3:67221204-67221223 | MS.gene84729:CDS | 75.0% | |
| CCTTCCACCACCCCCACCGC+CGG | + | chr8.3:67220830-67220849 | None:intergenic | 75.0% | |
| CGGCAACGGTTACGGCGGCG+GGG | - | chr8.3:67221205-67221224 | MS.gene84729:CDS | 75.0% | |
| !! | GCGGTGGGGGTGGTGGAAGG+AGG | - | chr8.3:67220830-67220849 | MS.gene84729:CDS | 75.0% |
| !!! | CCGGCGGTGGGGGTGGTGGA+AGG | - | chr8.3:67220827-67220846 | MS.gene84729:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67220490 | 67221761 | 67220490 | ID=MS.gene84729 |
| chr8.3 | mRNA | 67220490 | 67221761 | 67220490 | ID=MS.gene84729.t1;Parent=MS.gene84729 |
| chr8.3 | exon | 67220490 | 67221761 | 67220490 | ID=MS.gene84729.t1.exon1;Parent=MS.gene84729.t1 |
| chr8.3 | CDS | 67220490 | 67221761 | 67220490 | ID=cds.MS.gene84729.t1;Parent=MS.gene84729.t1 |
| Gene Sequence |
| Protein sequence |
