Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85033.t1 | RHN38641.1 | 78.1 | 105 | 22 | 1 | 10 | 114 | 2 | 105 | 4.30E-35 | 157.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85033.t1 | A0A396GE86 | 78.1 | 105 | 22 | 1 | 10 | 114 | 2 | 105 | 3.1e-35 | 157.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054236 | MS.gene85033 | 0.801534 | 8.61E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene85033 | 0.803764 | 2.98E-49 | -1.69E-46 |
| MS.gene05690 | MS.gene85033 | 0.813398 | 2.61E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene85033 | 0.806156 | 9.43E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene85033 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene85033 | 0.814287 | 1.66E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85033.t1 | MTR_4g115160 | 43.137 | 102 | 52 | 3 | 28 | 128 | 17 | 113 | 9.08e-18 | 78.2 |
| MS.gene85033.t1 | MTR_4g115160 | 43.137 | 102 | 52 | 3 | 28 | 128 | 17 | 113 | 1.07e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCCGATCCCCATAAAA+AGG | 0.218446 | 4.2:+67964421 | None:intergenic |
| TCTGCTTCGGCTCCGCCAAT+TGG | 0.225972 | 4.2:+67964459 | None:intergenic |
| CTTCATGAAAACGCTTATTA+AGG | 0.271400 | 4.2:-67964318 | MS.gene85033:CDS |
| CCTCGATTCTCCAACCCTTT+TGG | 0.315116 | 4.2:+67964164 | None:intergenic |
| ATTCAAATGGTTTGGATGTA+AGG | 0.320112 | 4.2:-67964277 | MS.gene85033:CDS |
| GATCGGGAATTATTAATATT+AGG | 0.323459 | 4.2:-67964409 | MS.gene85033:CDS |
| TTTGGATGTAAGGGAGATTA+TGG | 0.327543 | 4.2:-67964267 | MS.gene85033:CDS |
| CCATGTTGATTCAAATGGTT+TGG | 0.329157 | 4.2:-67964285 | MS.gene85033:CDS |
| CTGCTTCGGCTCCGCCAATT+GGG | 0.365388 | 4.2:+67964460 | None:intergenic |
| TCAAGTCTTTCCTGAGCAAT+TGG | 0.385721 | 4.2:-67964234 | MS.gene85033:CDS |
| ACAAACCATGTTGATTCAAA+TGG | 0.398939 | 4.2:-67964290 | MS.gene85033:CDS |
| CCTAACTGCATTTCCAAGTC+AGG | 0.437189 | 4.2:+67964374 | None:intergenic |
| GTTAATCAAACATTACCAAA+AGG | 0.440596 | 4.2:-67964179 | MS.gene85033:CDS |
| GAAGCAGGTGTGCCCTGACT+TGG | 0.455794 | 4.2:-67964387 | MS.gene85033:CDS |
| GTCTCATTTGTCTCGGAAGG+TGG | 0.457839 | 4.2:+67964482 | None:intergenic |
| TGAAGAGGTCACTAGAATCT+TGG | 0.477582 | 4.2:-67963537 | MS.gene85033:CDS |
| CCTGACTTGGAAATGCAGTT+AGG | 0.479556 | 4.2:-67964374 | MS.gene85033:CDS |
| AATTATTAATATTAGGAAGC+AGG | 0.496658 | 4.2:-67964402 | MS.gene85033:CDS |
| TTCAAATGGTTTGGATGTAA+GGG | 0.502250 | 4.2:-67964276 | MS.gene85033:CDS |
| CGAGACAAATGAGACCCAAT+TGG | 0.502821 | 4.2:-67964474 | MS.gene85033:CDS |
| TCAAACATTACCAAAAGGGT+TGG | 0.509562 | 4.2:-67964174 | MS.gene85033:CDS |
| TCTCATTTGTCTCGGAAGGT+GGG | 0.523843 | 4.2:+67964483 | None:intergenic |
| TGGGTCTCATTTGTCTCGGA+AGG | 0.527483 | 4.2:+67964479 | None:intergenic |
| TTAATCAAACATTACCAAAA+GGG | 0.530087 | 4.2:-67964178 | MS.gene85033:CDS |
| CAATTGGGTCTCATTTGTCT+CGG | 0.535680 | 4.2:+67964475 | None:intergenic |
| CCAAACCATTTGAATCAACA+TGG | 0.536609 | 4.2:+67964285 | None:intergenic |
| GTTAGGTAGCGTGAAGATGA+TGG | 0.538967 | 4.2:-67964357 | MS.gene85033:CDS |
| CGTGAAGATGATGGATGCTA+TGG | 0.564885 | 4.2:-67964348 | MS.gene85033:CDS |
| GACAAATGAGACCCAATTGG+CGG | 0.565179 | 4.2:-67964471 | MS.gene85033:CDS |
| AATTGGCGGAGCCGAAGCAG+AGG | 0.600793 | 4.2:-67964457 | MS.gene85033:CDS |
| TGCAGAGGTAGGTGATGAAG+AGG | 0.604398 | 4.2:-67963552 | MS.gene85033:intron |
| CCAAAAGGGTTGGAGAATCG+AGG | 0.640999 | 4.2:-67964164 | MS.gene85033:intron |
| CTAACTGCATTTCCAAGTCA+GGG | 0.648927 | 4.2:+67964375 | None:intergenic |
| AGGAAAGACTTGATTAACTG+CGG | 0.683094 | 4.2:+67964244 | None:intergenic |
| TAACAGCTTCTAAACCACGT+TGG | 0.692441 | 4.2:+67964198 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATATTTTTGTTTCTGTT+CGG | - | chr4.2:67963928-67963947 | MS.gene85033:intron | 15.0% |
| !!! | TATATTAAATCATGTCATTT+AGG | + | chr4.2:67963894-67963913 | None:intergenic | 15.0% |
| !! | AAGGTAGTTTATGAAAAAAA+CGG | + | chr4.2:67964193-67964212 | None:intergenic | 20.0% |
| !! | AATTATTAATATTAGGAAGC+AGG | - | chr4.2:67963618-67963637 | MS.gene85033:intron | 20.0% |
| !! | AGAAAATCATTGAAAACTAT+TGG | + | chr4.2:67964373-67964392 | None:intergenic | 20.0% |
| !! | TATAAGAATAATGAGTATCT+CGG | - | chr4.2:67964069-67964088 | MS.gene85033:intron | 20.0% |
| !! | TTAATCAAACATTACCAAAA+GGG | - | chr4.2:67963842-67963861 | MS.gene85033:intron | 20.0% |
| ! | GATCGGGAATTATTAATATT+AGG | - | chr4.2:67963611-67963630 | MS.gene85033:intron | 25.0% |
| ! | GTGTCTAATAGTTTGATAAA+AGG | + | chr4.2:67964096-67964115 | None:intergenic | 25.0% |
| ! | GTTAATCAAACATTACCAAA+AGG | - | chr4.2:67963841-67963860 | MS.gene85033:intron | 25.0% |
| ! | TATTATTCATAACCTTGTCA+AGG | + | chr4.2:67964212-67964231 | None:intergenic | 25.0% |
| ! | TCTGCTAAATACAACAAATT+TGG | + | chr4.2:67964118-67964137 | None:intergenic | 25.0% |
| !! | ACTCAAAAAACCCTTTTTAT+GGG | - | chr4.2:67963588-67963607 | MS.gene85033:intron | 25.0% |
| !! | TACTCAAAAAACCCTTTTTA+TGG | - | chr4.2:67963587-67963606 | MS.gene85033:intron | 25.0% |
| !! | TATTAGTTTAGCTTATGTGA+TGG | - | chr4.2:67964018-67964037 | MS.gene85033:intron | 25.0% |
| !!! | CTAATACATAAAAGCATCAA+TGG | + | chr4.2:67964004-67964023 | None:intergenic | 25.0% |
| ATAGTAAGGTGTAGTAACAA+AGG | + | chr4.2:67963977-67963996 | None:intergenic | 30.0% | |
| CTTCATGAAAACGCTTATTA+AGG | - | chr4.2:67963702-67963721 | MS.gene85033:intron | 30.0% | |
| TTTCATAAACTACCTTGACA+AGG | - | chr4.2:67964197-67964216 | MS.gene85033:CDS | 30.0% | |
| ! | ATTCAAATGGTTTGGATGTA+AGG | - | chr4.2:67963743-67963762 | MS.gene85033:intron | 30.0% |
| ! | CTCAAAAAACCCTTTTTATG+GGG | - | chr4.2:67963589-67963608 | MS.gene85033:intron | 30.0% |
| ! | TTCAAATGGTTTGGATGTAA+GGG | - | chr4.2:67963744-67963763 | MS.gene85033:intron | 30.0% |
| !! | ACAAACCATGTTGATTCAAA+TGG | - | chr4.2:67963730-67963749 | MS.gene85033:intron | 30.0% |
| AGGAAAGACTTGATTAACTG+CGG | + | chr4.2:67963779-67963798 | None:intergenic | 35.0% | |
| CCAAACCATTTGAATCAACA+TGG | + | chr4.2:67963738-67963757 | None:intergenic | 35.0% | |
| TCAAACATTACCAAAAGGGT+TGG | - | chr4.2:67963846-67963865 | MS.gene85033:intron | 35.0% | |
| TTATCCATAAGCTTGTGTCA+AGG | + | chr4.2:67964272-67964291 | None:intergenic | 35.0% | |
| TTTGGATGTAAGGGAGATTA+TGG | - | chr4.2:67963753-67963772 | MS.gene85033:intron | 35.0% | |
| ! | AAAACCCTTTTTATGGGGAT+CGG | - | chr4.2:67963594-67963613 | MS.gene85033:intron | 35.0% |
| !! | CCATGTTGATTCAAATGGTT+TGG | - | chr4.2:67963735-67963754 | MS.gene85033:intron | 35.0% |
| AATTCCCGATCCCCATAAAA+AGG | + | chr4.2:67963602-67963621 | None:intergenic | 40.0% | |
| ACTTCAGTTGTGATATGCAG+AGG | - | chr4.2:67964453-67964472 | MS.gene85033:CDS | 40.0% | |
| ATTCCCGATCCCCATAAAAA+GGG | + | chr4.2:67963601-67963620 | None:intergenic | 40.0% | |
| CAATTGGGTCTCATTTGTCT+CGG | + | chr4.2:67963548-67963567 | None:intergenic | 40.0% | |
| CTAACTGCATTTCCAAGTCA+GGG | + | chr4.2:67963648-67963667 | None:intergenic | 40.0% | |
| GCATCAATGGTGTGATAGTA+AGG | + | chr4.2:67963991-67964010 | None:intergenic | 40.0% | |
| TAACAGCTTCTAAACCACGT+TGG | + | chr4.2:67963825-67963844 | None:intergenic | 40.0% | |
| TCAAGTCTTTCCTGAGCAAT+TGG | - | chr4.2:67963786-67963805 | MS.gene85033:intron | 40.0% | |
| TCTACCTTGACACAAGCTTA+TGG | - | chr4.2:67964265-67964284 | MS.gene85033:CDS | 40.0% | |
| TGAAGAGGTCACTAGAATCT+TGG | - | chr4.2:67964483-67964502 | MS.gene85033:CDS | 40.0% | |
| ! | AAACCCTTTTTATGGGGATC+GGG | - | chr4.2:67963595-67963614 | MS.gene85033:intron | 40.0% |
| !!! | TTTTTGAGTAGCCTCTGCTT+CGG | + | chr4.2:67963577-67963596 | None:intergenic | 40.0% |
| CAGTTGTGATATGCAGAGGT+AGG | - | chr4.2:67964457-67964476 | MS.gene85033:CDS | 45.0% | |
| CCTAACTGCATTTCCAAGTC+AGG | + | chr4.2:67963649-67963668 | None:intergenic | 45.0% | |
| CGAGACAAATGAGACCCAAT+TGG | - | chr4.2:67963546-67963565 | MS.gene85033:CDS | 45.0% | |
| GACAAATGAGACCCAATTGG+CGG | - | chr4.2:67963549-67963568 | MS.gene85033:CDS | 45.0% | |
| GTTAGGTAGCGTGAAGATGA+TGG | - | chr4.2:67963663-67963682 | MS.gene85033:intron | 45.0% | |
| TCTCATTTGTCTCGGAAGGT+GGG | + | chr4.2:67963540-67963559 | None:intergenic | 45.0% | |
| !! | ACCACGTTGGTGAGCATTTT+TGG | + | chr4.2:67963812-67963831 | None:intergenic | 45.0% |
| !! | CCTGACTTGGAAATGCAGTT+AGG | - | chr4.2:67963646-67963665 | MS.gene85033:intron | 45.0% |
| !! | CGTGAAGATGATGGATGCTA+TGG | - | chr4.2:67963672-67963691 | MS.gene85033:intron | 45.0% |
| !!! | GCATTTTTGGCCAATTGCTC+AGG | + | chr4.2:67963799-67963818 | None:intergenic | 45.0% |
| CCAAAAGGGTTGGAGAATCG+AGG | - | chr4.2:67963856-67963875 | MS.gene85033:intron | 50.0% | |
| GCCAAAAATGCTCACCAACG+TGG | - | chr4.2:67963808-67963827 | MS.gene85033:intron | 50.0% | |
| GTCTCATTTGTCTCGGAAGG+TGG | + | chr4.2:67963541-67963560 | None:intergenic | 50.0% | |
| TGCAGAGGTAGGTGATGAAG+AGG | - | chr4.2:67964468-67964487 | MS.gene85033:CDS | 50.0% | |
| TGGGTCTCATTTGTCTCGGA+AGG | + | chr4.2:67963544-67963563 | None:intergenic | 50.0% | |
| ! | CCTCGATTCTCCAACCCTTT+TGG | + | chr4.2:67963859-67963878 | None:intergenic | 50.0% |
| AATTGGCGGAGCCGAAGCAG+AGG | - | chr4.2:67963563-67963582 | MS.gene85033:intron | 60.0% | |
| CTGCTTCGGCTCCGCCAATT+GGG | + | chr4.2:67963563-67963582 | None:intergenic | 60.0% | |
| GAAGCAGGTGTGCCCTGACT+TGG | - | chr4.2:67963633-67963652 | MS.gene85033:intron | 60.0% | |
| TCTGCTTCGGCTCCGCCAAT+TGG | + | chr4.2:67963564-67963583 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 67963520 | 67964522 | 67963520 | ID=MS.gene85033 |
| chr4.2 | mRNA | 67963520 | 67964522 | 67963520 | ID=MS.gene85033.t1;Parent=MS.gene85033 |
| chr4.2 | exon | 67964165 | 67964522 | 67964165 | ID=MS.gene85033.t1.exon1;Parent=MS.gene85033.t1 |
| chr4.2 | CDS | 67964165 | 67964522 | 67964165 | ID=cds.MS.gene85033.t1;Parent=MS.gene85033.t1 |
| chr4.2 | exon | 67963520 | 67963569 | 67963520 | ID=MS.gene85033.t1.exon2;Parent=MS.gene85033.t1 |
| chr4.2 | CDS | 67963520 | 67963569 | 67963520 | ID=cds.MS.gene85033.t1;Parent=MS.gene85033.t1 |
| Gene Sequence |
| Protein sequence |
