AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85228.t1 MTR_2g099780 94.390 410 15 2 1 408 1 404 0.0 682
MS.gene85228.t1 MTR_8g017570 53.273 443 160 14 1 407 1 432 9.01e-108 325
MS.gene85228.t1 MTR_3g104570 46.154 442 185 13 1 399 1 432 4.16e-84 264
MS.gene85228.t1 MTR_4g050480 41.697 271 134 6 1 257 1 261 7.86e-38 142
MS.gene85228.t1 MTR_4g050480 41.544 272 134 6 1 257 1 262 5.54e-37 140
MS.gene85228.t1 MTR_6g033415 35.974 303 155 7 80 353 105 397 5.60e-29 117
MS.gene85228.t1 MTR_8g024540 41.451 193 85 7 80 257 125 304 1.40e-28 117
MS.gene85228.t1 MTR_1g095740 37.989 179 99 5 81 256 140 309 4.65e-27 112
MS.gene85228.t1 MTR_1g066800 39.896 193 98 7 59 249 104 280 1.29e-26 111
MS.gene85228.t1 MTR_1g066800 39.896 193 98 7 59 249 99 275 1.37e-26 111
MS.gene85228.t1 MTR_7g100790 31.287 342 164 10 56 346 88 409 1.44e-25 108
MS.gene85228.t1 MTR_1g103070 30.522 249 106 6 1 182 1 249 7.53e-22 97.4
MS.gene85228.t1 MTR_0047s0040 38.060 134 74 3 81 208 100 230 2.77e-21 95.5
MS.gene85228.t1 MTR_2g078210 34.659 176 99 4 84 256 16 178 3.12e-21 92.8
MS.gene85228.t1 MTR_2g078210 34.659 176 99 4 84 256 76 238 3.15e-21 94.0
MS.gene85228.t1 MTR_2g015370 29.885 261 152 6 1 261 1 230 4.54e-21 93.2
MS.gene85228.t1 MTR_3g020990 41.593 113 58 2 95 206 103 208 1.89e-19 87.4
MS.gene85228.t1 MTR_4g108140 34.659 176 92 4 1 156 1 173 8.72e-19 89.0
MS.gene85228.t1 MTR_4g108140 35.593 177 90 5 1 156 1 174 8.74e-19 89.0
MS.gene85228.t1 MTR_1g041430 33.772 228 100 6 1 180 1 225 1.42e-18 87.4
MS.gene85228.t1 MTR_3g091560 31.206 282 144 9 82 346 112 360 3.32e-18 86.7
MS.gene85228.t1 MTR_8g014580 55.072 69 31 0 80 148 124 192 5.44e-18 85.9
MS.gene85228.t1 MTR_1g007920 39.583 192 97 5 80 252 120 311 6.43e-18 85.9
MS.gene85228.t1 MTR_1g007920 39.583 192 97 5 80 252 95 286 6.90e-18 85.5
MS.gene85228.t1 MTR_4g102840 37.333 150 84 3 1 142 1 148 9.12e-18 85.1
MS.gene85228.t1 MTR_8g074730 53.623 69 32 0 77 145 66 134 2.90e-17 78.6
MS.gene85228.t1 MTR_8g102400 39.205 176 77 6 1 146 1 176 5.96e-17 82.8
MS.gene85228.t1 MTR_4g087420 31.724 145 83 2 78 210 142 282 6.81e-17 82.8
MS.gene85228.t1 MTR_0019s0070 62.903 62 23 0 84 145 119 180 1.00e-16 82.0
MS.gene85228.t1 MTR_2g100740 33.511 188 100 5 1 163 1 188 2.18e-16 80.5
MS.gene85228.t1 MTR_7g114870 31.148 183 84 5 1 150 1 174 2.63e-16 80.5
MS.gene85228.t1 MTR_1g051810 26.403 303 170 10 84 373 107 369 2.89e-16 80.1
MS.gene85228.t1 MTR_1g101330 56.061 66 29 0 77 142 110 175 5.19e-16 79.7
MS.gene85228.t1 MTR_7g114700 50.000 66 33 0 81 146 134 199 3.21e-15 77.4
MS.gene85228.t1 MTR_5g038620 38.614 101 62 0 60 160 97 197 4.01e-13 71.6
MS.gene85228.t1 MTR_1g023760 50.746 67 33 0 82 148 124 190 9.02e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85228.t1 AT1G72670 46.698 424 165 18 1 392 1 395 4.52e-88 273
MS.gene85228.t1 AT2G26180 48.095 420 168 13 1 389 1 401 2.20e-85 266
MS.gene85228.t1 AT1G17480 41.758 364 161 12 1 343 1 334 1.02e-62 206
MS.gene85228.t1 AT5G03040 39.904 208 115 5 48 253 80 279 1.12e-32 128
MS.gene85228.t1 AT5G03040 39.904 208 115 5 48 253 80 279 1.12e-32 128
MS.gene85228.t1 AT5G03040 39.904 208 115 5 48 253 80 279 1.12e-32 128
MS.gene85228.t1 AT3G22190 48.901 182 77 4 80 257 86 255 7.32e-32 125
MS.gene85228.t1 AT3G22190 48.901 182 77 4 80 257 86 255 7.32e-32 125
MS.gene85228.t1 AT3G22190 48.901 182 77 4 80 257 86 255 7.32e-32 125
MS.gene85228.t1 AT3G22190 48.901 182 77 4 80 257 86 255 7.32e-32 125
MS.gene85228.t1 AT1G01110 36.232 207 109 5 72 264 111 308 7.83e-27 112
MS.gene85228.t1 AT4G00820 38.095 189 89 5 80 252 130 306 4.40e-26 110
MS.gene85228.t1 AT3G52290 37.572 173 97 4 81 252 107 269 6.55e-25 106
MS.gene85228.t1 AT3G09710 37.017 181 99 6 80 257 106 274 2.05e-24 105
MS.gene85228.t1 AT3G49260 35.519 183 97 5 84 252 122 297 3.60e-24 104
MS.gene85228.t1 AT3G49260 35.519 183 96 6 84 252 122 296 5.58e-24 103
MS.gene85228.t1 AT3G49260 35.519 183 96 6 84 252 122 296 5.58e-24 103
MS.gene85228.t1 AT3G49260 35.519 183 96 6 84 252 122 296 5.58e-24 103
MS.gene85228.t1 AT3G09710 34.896 192 102 5 80 257 106 288 7.06e-24 103
MS.gene85228.t1 AT4G10640 28.796 382 190 12 7 346 18 359 3.97e-23 100
MS.gene85228.t1 AT4G14750 28.819 288 150 10 1 250 1 271 1.01e-22 99.4
MS.gene85228.t1 AT4G14750 28.819 288 150 10 1 250 60 330 1.73e-22 99.4
MS.gene85228.t1 AT4G14750 28.819 288 150 10 1 250 60 330 1.93e-22 99.4
MS.gene85228.t1 AT3G59690 37.324 142 75 3 73 210 161 292 1.01e-20 94.7
MS.gene85228.t1 AT3G59690 37.324 142 75 3 73 210 161 292 1.01e-20 94.7
MS.gene85228.t1 AT3G15050 31.429 210 119 5 84 284 52 245 1.61e-20 90.9
MS.gene85228.t1 AT2G26410 34.054 185 103 5 80 257 146 318 6.20e-20 92.4
MS.gene85228.t1 AT2G26410 34.054 185 103 5 80 257 146 318 6.20e-20 92.4
MS.gene85228.t1 AT2G33990 31.818 176 109 3 81 256 46 210 8.53e-19 85.9
MS.gene85228.t1 AT1G18840 45.745 94 47 1 79 168 106 199 4.55e-18 86.7
MS.gene85228.t1 AT1G18840 45.745 94 47 1 79 168 106 199 4.55e-18 86.7
MS.gene85228.t1 AT1G18840 45.745 94 47 1 79 168 106 199 4.55e-18 86.7
MS.gene85228.t1 AT4G23060 58.108 74 31 0 82 155 167 240 5.41e-18 86.3
MS.gene85228.t1 AT4G29150 42.500 120 61 2 70 181 119 238 1.73e-17 84.0
MS.gene85228.t1 AT5G13460 36.552 145 71 4 76 208 103 238 5.30e-17 82.8
MS.gene85228.t1 AT1G51960 50.000 80 37 2 70 148 89 166 7.40e-17 81.6
MS.gene85228.t1 AT3G51380 57.576 66 28 0 80 145 35 100 1.03e-16 75.9
MS.gene85228.t1 AT3G16490 44.565 92 49 1 81 170 106 197 1.10e-16 81.6
MS.gene85228.t1 AT5G07240 28.819 288 147 9 81 364 104 337 2.71e-16 80.5
MS.gene85228.t1 AT5G07240 28.819 288 147 9 81 364 150 383 4.81e-16 80.1
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.33e-15 79.3
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.33e-15 79.3
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.33e-15 79.3
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.80e-15 79.0
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.80e-15 79.0
MS.gene85228.t1 AT1G14380 34.356 163 89 4 1 148 1 160 1.80e-15 79.0
MS.gene85228.t1 AT1G14380 33.750 160 94 3 1 148 1 160 2.17e-15 78.6
MS.gene85228.t1 AT1G74690 51.429 70 34 0 79 148 110 179 2.17e-15 78.6
MS.gene85228.t1 AT5G03960 38.060 134 80 2 84 214 110 243 2.12e-14 74.7
MS.gene85228.t1 AT5G03960 38.060 134 80 2 84 214 110 243 2.12e-14 74.7
MS.gene85228.t1 AT5G03960 38.060 134 80 2 84 214 109 242 2.17e-14 74.7
MS.gene85228.t1 AT2G43680 33.099 142 88 4 67 208 311 445 2.49e-14 75.5
MS.gene85228.t1 AT2G43680 33.099 142 88 4 67 208 310 444 2.51e-14 75.5
MS.gene85228.t1 AT2G43680 33.099 142 88 4 67 208 310 444 2.51e-14 75.5
MS.gene85228.t1 AT2G43680 33.099 142 88 4 67 208 310 444 2.51e-14 75.5
MS.gene85228.t1 AT2G43680 33.099 142 88 4 67 208 310 444 2.51e-14 75.5
MS.gene85228.t1 AT1G19870 35.338 133 68 3 60 176 177 307 1.06e-13 73.6
MS.gene85228.t1 AT1G19870 38.938 113 56 2 75 176 14 124 1.17e-13 73.2
MS.gene85228.t1 AT3G49380 36.134 119 65 2 27 138 52 166 2.04e-13 71.6
MS.gene85228.t1 AT5G62070 64.151 53 19 0 84 136 119 171 5.87e-13 70.5

Find 78 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGACCCTTCAGTTAATTTA+TGG 0.180664 2.4:+4517957 MS.gene85228:CDS
CAATGGGTGTTTCTGGAAAA+TGG 0.187083 2.4:+4515580 None:intergenic
TGCTGCACTGCTTTGCCTTC+TGG 0.231347 2.4:-4517059 None:intergenic
GGCTCTCTTTCGAGCCTTCT+TGG 0.249229 2.4:+4515934 MS.gene85228:CDS
TGATAATAACTTGCCCTTAG+AGG 0.285753 2.4:-4517998 None:intergenic
CTAAAGATTTCATCCTTATC+AGG 0.290356 2.4:+4515885 MS.gene85228:CDS
TCACTCTTCCTGCTTGAGAT+TGG 0.302121 2.4:-4517581 None:intergenic
TTAACTTCATCTGCAGTTCC+AGG 0.344133 2.4:-4517269 None:intergenic
GCAAGCTCTGGTTAGGGTTC+AGG 0.355190 2.4:+4517004 MS.gene85228:CDS
ATGGGCTGCTATTCGCATTC+AGG 0.358781 2.4:+4515913 MS.gene85228:CDS
CTGATAAGGATGAAATCTTT+AGG 0.373735 2.4:-4515884 None:intergenic
AACAAGCAGCTGTTACTCTA+AGG 0.378838 2.4:+4516976 MS.gene85228:CDS
GTAATAGTACTAAGAAGAAA+TGG 0.387683 2.4:+4515735 MS.gene85228:CDS
AGGCTAAGCTCAAAACTTAC+AGG 0.390491 2.4:+4517831 MS.gene85228:CDS
GGTGTATGCAAGCTCTGGTT+AGG 0.390529 2.4:+4516997 MS.gene85228:CDS
TTATCAACTTCATGTACATC+TGG 0.395939 2.4:+4517731 MS.gene85228:CDS
ATCGTTGTGCAACACCTCTA+AGG 0.396953 2.4:+4517984 MS.gene85228:CDS
AGCAGCACCAGTCTTCTTCA+TGG 0.398235 2.4:-4515792 None:intergenic
CAAGGATGGTGTGAAATTCC+TGG 0.403362 2.4:+4517251 MS.gene85228:CDS
GATAATAGTATAAACAGTTC+TGG 0.410910 2.4:+4517932 MS.gene85228:CDS
TTCCTTCTTGATTGAAAAGT+AGG 0.412386 2.4:-4517614 None:intergenic
CATTGCTCTATCCCTCTTGA+TGG 0.414300 2.4:-4517321 None:intergenic
TTCCTAAGCTCTTGTTGTCA+TGG 0.417603 2.4:-4517508 None:intergenic
AGTTATTATCAAAATCGATA+TGG 0.428695 2.4:+4518010 MS.gene85228:CDS
CAGCCCATTCCTGCCTGATA+AGG 0.431812 2.4:-4515898 None:intergenic
CATCCTTATCAGGCAGGAAT+GGG 0.450099 2.4:+4515895 MS.gene85228:CDS
GTTGCTGTCGTTGTTCGAGC+TGG 0.450476 2.4:+4515860 MS.gene85228:CDS
AAGAGCTTTGAGAGCATTGA+GGG 0.454446 2.4:+4516908 MS.gene85228:CDS
CTAAGAAGAAATGGAAGCTA+TGG 0.454548 2.4:+4515744 MS.gene85228:CDS
TCATCCTTATCAGGCAGGAA+TGG 0.473411 2.4:+4515894 MS.gene85228:CDS
AGACTGCAAGCAATATTTAG+AGG 0.495590 2.4:+4516939 MS.gene85228:CDS
GAAATAGCTTGTTAGAGCGC+TGG 0.496586 2.4:+4517528 MS.gene85228:CDS
TGCAAGCAATATTTAGAGGC+AGG 0.511357 2.4:+4516943 MS.gene85228:CDS
TCTAAGGTGTATGCAAGCTC+TGG 0.513287 2.4:+4516992 MS.gene85228:CDS
GAAGGAATGGTGTGACAACT+AGG 0.517271 2.4:+4517630 MS.gene85228:CDS
AGATTTCATCCTTATCAGGC+AGG 0.519223 2.4:+4515889 MS.gene85228:CDS
ATCATCTTCGACTTTAAGCT+CGG 0.520897 2.4:+4517685 MS.gene85228:CDS
GTGTATGCAAGCTCTGGTTA+GGG 0.521748 2.4:+4516998 MS.gene85228:CDS
GTTATTATTATCAGCAAGGA+TGG 0.522365 2.4:+4517237 MS.gene85228:intron
GGCAAGAAGGTGCCATCAAG+AGG 0.522656 2.4:+4517309 MS.gene85228:CDS
TCTTCAACCATGAAGAAGAC+TGG 0.527272 2.4:+4515785 MS.gene85228:CDS
TGGCAAACAAACCATGTCCA+TGG 0.529332 2.4:+4517552 MS.gene85228:CDS
TGATTGTTAATTTACCTGAT+CGG 0.529601 2.4:-4515631 None:intergenic
GGCAGCAGCAGTATCGGTAA+TGG 0.530108 2.4:-4515837 None:intergenic
CCCTTCAGTTAATTTATGGA+AGG 0.533849 2.4:+4517961 MS.gene85228:CDS
TTCAGGCACGTGTGAGAGCT+AGG 0.537833 2.4:+4517021 MS.gene85228:CDS
AACCATGACAACAAGAGCTT+AGG 0.537945 2.4:+4517506 MS.gene85228:CDS
AGCAACGGCAGCAGCAGTAT+CGG 0.538131 2.4:-4515843 None:intergenic
AAGCTAAGAATGAGGCAAGA+AGG 0.538231 2.4:+4517296 MS.gene85228:CDS
TCAAATTCATGTAGCTAGGT+AGG 0.539947 2.4:-4517796 None:intergenic
GAGCTTAAAGTCGAAGATGA+TGG 0.542387 2.4:-4517683 None:intergenic
TCCTTCCATAAATTAACTGA+AGG 0.545088 2.4:-4517962 None:intergenic
ATTGGACTCTCCCATGGACA+TGG 0.548204 2.4:-4517563 None:intergenic
CAGAATCAGCATGGTTGCGA+TGG 0.549893 2.4:-4517094 None:intergenic
ATTATCAAAATCGATATGGC+AGG 0.555854 2.4:+4518014 MS.gene85228:CDS
TCGTTGTGCAACACCTCTAA+GGG 0.556401 2.4:+4517985 MS.gene85228:CDS
CTTGAGATTGGACTCTCCCA+TGG 0.558321 2.4:-4517569 None:intergenic
AAAAGAAATAGTTACCAAGA+AGG 0.563052 2.4:-4515948 None:intergenic
TTTGAGAGCATTGAGGGCAG+TGG 0.564489 2.4:+4516914 MS.gene85228:CDS
GAAGAGCTTTGAGAGCATTG+AGG 0.569321 2.4:+4516907 MS.gene85228:CDS
GAGTTCAAAGAGACTCGTAA+TGG 0.575348 2.4:+4517865 MS.gene85228:CDS
CCTTCCATAAATTAACTGAA+GGG 0.587260 2.4:-4517961 None:intergenic
TCTGTCAAATTCATGTAGCT+AGG 0.592487 2.4:-4517800 None:intergenic
GAATTTGACAGAATCAACTA+AGG 0.594832 2.4:+4517811 MS.gene85228:CDS
ATCTATGCCAGAAGCAACAG+AGG 0.595241 2.4:+4517760 MS.gene85228:CDS
GGGAGAGTCCAATCTCAAGC+AGG 0.595864 2.4:+4517573 MS.gene85228:CDS
GGCAAACAAACCATGTCCAT+GGG 0.598464 2.4:+4517553 MS.gene85228:CDS
TTAAAGCAAAGCTAAGAATG+AGG 0.599503 2.4:+4517288 MS.gene85228:CDS
TGATTCTGTTAAGCTTGTAG+AGG 0.611493 2.4:+4517109 MS.gene85228:CDS
AGCTCGAACAACGACAGCAA+CGG 0.615911 2.4:-4515858 None:intergenic
CATCAAGAGGGATAGAGCAA+TGG 0.627816 2.4:+4517322 MS.gene85228:CDS
TAGCTTGTTAGAGCGCTGGA+TGG 0.632949 2.4:+4517532 MS.gene85228:CDS
TCAACACTTAATTACCTGTG+TGG 0.634403 2.4:-4517357 None:intergenic
GCAAGAAGGTGCCATCAAGA+GGG 0.646053 2.4:+4517310 MS.gene85228:CDS
GTGTGAGAGCTAGGAATGTG+AGG 0.652883 2.4:+4517030 MS.gene85228:CDS
CAAGCTTAACAGAATCAGCA+TGG 0.655634 2.4:-4517103 None:intergenic
GGCATACTCTCTATCCACAC+AGG 0.672618 2.4:+4517343 MS.gene85228:CDS
AATGTGAGGAAATCTCCAGA+AGG 0.698682 2.4:+4517044 MS.gene85228:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAGAAAGAAAAAATAA+AGG - chr2.4:4516610-4516629 None:intergenic 15.0%
!! AAAGAGAAAGAAAAAATAAA+GGG - chr2.4:4516609-4516628 None:intergenic 15.0%
!! ATTACATAATATTCTCTAAA+TGG + chr2.4:4517160-4517179 MS.gene85228:intron 15.0%
!! TAAAAAGTTGAATTTCAATT+AGG - chr2.4:4516557-4516576 None:intergenic 15.0%
!!! ATTCAAATTCAATTTTTTTG+AGG - chr2.4:4516170-4516189 None:intergenic 15.0%
!!! TTATTTTTTCTTTCTCTTTT+TGG + chr2.4:4516610-4516629 MS.gene85228:intron 15.0%
!!! TTCTTTTTAAAATACATTGA+AGG + chr2.4:4516881-4516900 MS.gene85228:intron 15.0%
!! AAGAGAAAGAAAAAATAAAG+GGG - chr2.4:4516608-4516627 None:intergenic 20.0%
!! AGTTATTATCAAAATCGATA+TGG + chr2.4:4518010-4518029 MS.gene85228:CDS 20.0%
!! ATATTCTCTAAATGGTTATT+TGG + chr2.4:4517168-4517187 MS.gene85228:intron 20.0%
!! ATTAACACAACAACAAAAAA+AGG - chr2.4:4515678-4515697 None:intergenic 20.0%
!! GCAATAAATCCTAAAAAAAT+GGG - chr2.4:4516760-4516779 None:intergenic 20.0%
!! TATAGTATATGCTAACATAA+GGG + chr2.4:4517202-4517221 MS.gene85228:intron 20.0%
!! TGCAATAAATCCTAAAAAAA+TGG - chr2.4:4516761-4516780 None:intergenic 20.0%
!! TTATAGTATATGCTAACATA+AGG + chr2.4:4517201-4517220 MS.gene85228:intron 20.0%
!!! AAATTCAATTTTTTTGAGGA+AGG - chr2.4:4516166-4516185 None:intergenic 20.0%
! AAAAAAAAAGTAACCAAGAG+TGG - chr2.4:4516794-4516813 None:intergenic 25.0%
! AAAAGAAATAGTTACCAAGA+AGG - chr2.4:4515951-4515970 None:intergenic 25.0%
! AAAAGCAAGCAAAGATATAT+TGG - chr2.4:4517414-4517433 None:intergenic 25.0%
! AAGTTTACATGACTTAAAAG+AGG - chr2.4:4516208-4516227 None:intergenic 25.0%
! GATAATAGTATAAACAGTTC+TGG + chr2.4:4517932-4517951 MS.gene85228:CDS 25.0%
! GTAATAGTACTAAGAAGAAA+TGG + chr2.4:4515735-4515754 MS.gene85228:CDS 25.0%
! GTTGAATTTCAATTAGGTAA+AGG - chr2.4:4516551-4516570 None:intergenic 25.0%
! TAAAACAATGTGAAAAAGAG+TGG - chr2.4:4516414-4516433 None:intergenic 25.0%
! TACAAGATCTACTAATAGTT+GGG + chr2.4:4516083-4516102 MS.gene85228:intron 25.0%
! TGATTGTTAATTTACCTGAT+CGG - chr2.4:4515634-4515653 None:intergenic 25.0%
! TTACAAGATCTACTAATAGT+TGG + chr2.4:4516082-4516101 MS.gene85228:intron 25.0%
! TTATAACTCAGTTATGCATT+TGG - chr2.4:4516353-4516372 None:intergenic 25.0%
! TTCAAATCATGAAAACACAA+AGG - chr2.4:4516131-4516150 None:intergenic 25.0%
!! TTTCTTTCTCTTTTTGGATT+GGG + chr2.4:4516616-4516635 MS.gene85228:intron 25.0%
!! TTTTCTTTCTCTTTTTGGAT+TGG + chr2.4:4516615-4516634 MS.gene85228:intron 25.0%
!!! GCTAATATTTCTGCAATTTT+TGG - chr2.4:4516392-4516411 None:intergenic 25.0%
AAATCATTCAACCCCAATAA+AGG - chr2.4:4516245-4516264 None:intergenic 30.0%
AACCCCAATAAAGGAAAAAA+GGG - chr2.4:4516236-4516255 None:intergenic 30.0%
AATTATGGATCAGAATTCAG+GGG + chr2.4:4516041-4516060 MS.gene85228:intron 30.0%
AGAAAGAAAAAATAAAGGGG+AGG - chr2.4:4516605-4516624 None:intergenic 30.0%
ATCTACTAATAGTTGGGATT+TGG + chr2.4:4516089-4516108 MS.gene85228:intron 30.0%
ATTATCAAAATCGATATGGC+AGG + chr2.4:4518014-4518033 MS.gene85228:CDS 30.0%
CAATTATGGATCAGAATTCA+GGG + chr2.4:4516040-4516059 MS.gene85228:intron 30.0%
CCATGTTATTATTATCAGCA+AGG + chr2.4:4517233-4517252 MS.gene85228:intron 30.0%
CCTTCCATAAATTAACTGAA+GGG - chr2.4:4517964-4517983 None:intergenic 30.0%
CCTTGCTGATAATAATAACA+TGG - chr2.4:4517236-4517255 None:intergenic 30.0%
CTAAAGATTTCATCCTTATC+AGG + chr2.4:4515885-4515904 MS.gene85228:CDS 30.0%
CTGATAAGGATGAAATCTTT+AGG - chr2.4:4515887-4515906 None:intergenic 30.0%
GAATTTGACAGAATCAACTA+AGG + chr2.4:4517811-4517830 MS.gene85228:CDS 30.0%
GGATTTGGGAGTTTAAAAAA+TGG + chr2.4:4516104-4516123 MS.gene85228:intron 30.0%
GTTATTATTATCAGCAAGGA+TGG + chr2.4:4517237-4517256 MS.gene85228:intron 30.0%
GTTGAATGATTTAGGTAGTT+AGG + chr2.4:4516253-4516272 MS.gene85228:intron 30.0%
TCCTTCCATAAATTAACTGA+AGG - chr2.4:4517965-4517984 None:intergenic 30.0%
TCTACTAATAGTTGGGATTT+GGG + chr2.4:4516090-4516109 MS.gene85228:intron 30.0%
TGAAATTCCAAGTCCAATTA+TGG + chr2.4:4516026-4516045 MS.gene85228:intron 30.0%
TTAAAGCAAAGCTAAGAATG+AGG + chr2.4:4517288-4517307 MS.gene85228:CDS 30.0%
TTATCAACTTCATGTACATC+TGG + chr2.4:4517731-4517750 MS.gene85228:CDS 30.0%
TTATTGGGGTTGAATGATTT+AGG + chr2.4:4516245-4516264 MS.gene85228:intron 30.0%
TTCCAATAAAAAAACCGATC+AGG + chr2.4:4515617-4515636 MS.gene85228:CDS 30.0%
TTCCTTCTTGATTGAAAAGT+AGG - chr2.4:4517617-4517636 None:intergenic 30.0%
! AATGGTTATTTGGAGTTACA+AGG + chr2.4:4517178-4517197 MS.gene85228:intron 30.0%
! ACAAGTACAACTTTTCTGAA+TGG + chr2.4:4517908-4517927 MS.gene85228:CDS 30.0%
! ACTTTTCAATCAAGAAGGAA+TGG + chr2.4:4517617-4517636 MS.gene85228:CDS 30.0%
! AGGATTTTACAGTCATCTTA+AGG - chr2.4:4516585-4516604 None:intergenic 30.0%
! AGTGTTAATGTTGTTTGTTC+AGG + chr2.4:4515703-4515722 MS.gene85228:intron 30.0%
! ATTTTACAGTCATCTTAAGG+AGG - chr2.4:4516582-4516601 None:intergenic 30.0%
! GTAACTTAGGTTCTTTTGTA+TGG + chr2.4:4516666-4516685 MS.gene85228:intron 30.0%
! TAGGTTCTTTTGTATGGTTA+TGG + chr2.4:4516272-4516291 MS.gene85228:intron 30.0%
! TTCTTTCTCTTTTTGGATTG+GGG + chr2.4:4516617-4516636 MS.gene85228:intron 30.0%
!! TTCCTACTTTTCAATCAAGA+AGG + chr2.4:4517612-4517631 MS.gene85228:CDS 30.0%
!!! GATTTTGTGGATTTTGAGTT+TGG - chr2.4:4517461-4517480 None:intergenic 30.0%
!!! TAAACACTGCCCATTTTTTT+AGG + chr2.4:4516748-4516767 MS.gene85228:intron 30.0%
!!! TACCTGATCGGTTTTTTTAT+TGG - chr2.4:4515622-4515641 None:intergenic 30.0%
ACCCCAATAAAGGAAAAAAG+GGG - chr2.4:4516235-4516254 None:intergenic 35.0%
AGACTGCAAGCAATATTTAG+AGG + chr2.4:4516939-4516958 MS.gene85228:CDS 35.0%
ATCATCTTCGACTTTAAGCT+CGG + chr2.4:4517685-4517704 MS.gene85228:CDS 35.0%
ATGGTTATGGTAATATGCCA+GGG + chr2.4:4516285-4516304 MS.gene85228:intron 35.0%
CAACCCCAATAAAGGAAAAA+AGG - chr2.4:4516237-4516256 None:intergenic 35.0%
CCAATTATGGATCAGAATTC+AGG + chr2.4:4516039-4516058 MS.gene85228:intron 35.0%
CCACAGCAAAAATCATAGAA+AGG - chr2.4:4515974-4515993 None:intergenic 35.0%
CCCTTCAGTTAATTTATGGA+AGG + chr2.4:4517961-4517980 MS.gene85228:CDS 35.0%
CCTGAATTCTGATCCATAAT+TGG - chr2.4:4516042-4516061 None:intergenic 35.0%
CTAAGAAGAAATGGAAGCTA+TGG + chr2.4:4515744-4515763 MS.gene85228:CDS 35.0%
CTGACCCTTCAGTTAATTTA+TGG + chr2.4:4517957-4517976 MS.gene85228:CDS 35.0%
CTTCAGTAGTTAGGTAACTT+AGG + chr2.4:4516653-4516672 MS.gene85228:intron 35.0%
TATGGTTATGGTAATATGCC+AGG + chr2.4:4516284-4516303 MS.gene85228:intron 35.0%
TCAAATTCATGTAGCTAGGT+AGG - chr2.4:4517799-4517818 None:intergenic 35.0%
TCAACACTTAATTACCTGTG+TGG - chr2.4:4517360-4517379 None:intergenic 35.0%
TCTGTCAAATTCATGTAGCT+AGG - chr2.4:4517803-4517822 None:intergenic 35.0%
TGATTCTGTTAAGCTTGTAG+AGG + chr2.4:4517109-4517128 MS.gene85228:CDS 35.0%
TTCTGATCCATAATTGGACT+TGG - chr2.4:4516036-4516055 None:intergenic 35.0%
! CCTTTCTATGATTTTTGCTG+TGG + chr2.4:4515971-4515990 MS.gene85228:intron 35.0%
! GGTAGTTAGGTTCTTTTGTA+TGG + chr2.4:4516266-4516285 MS.gene85228:intron 35.0%
! TGATAATAACTTGCCCTTAG+AGG - chr2.4:4518001-4518020 None:intergenic 35.0%
!! TCTTTCTCTTTTTGGATTGG+GGG + chr2.4:4516618-4516637 MS.gene85228:intron 35.0%
!!! GAAAAGAGACACTGATTTTG+TGG - chr2.4:4517474-4517493 None:intergenic 35.0%
!!! TCCCCCTTTTTTCCTTTATT+GGG + chr2.4:4516230-4516249 MS.gene85228:intron 35.0%
!!! TTCCCCCTTTTTTCCTTTAT+TGG + chr2.4:4516229-4516248 MS.gene85228:intron 35.0%
AACAAGCAGCTGTTACTCTA+AGG + chr2.4:4516976-4516995 MS.gene85228:CDS 40.0%
AACCATGACAACAAGAGCTT+AGG + chr2.4:4517506-4517525 MS.gene85228:CDS 40.0%
AAGCTAAGAATGAGGCAAGA+AGG + chr2.4:4517296-4517315 MS.gene85228:CDS 40.0%
AATGTGAGGAAATCTCCAGA+AGG + chr2.4:4517044-4517063 MS.gene85228:CDS 40.0%
AGATTTCATCCTTATCAGGC+AGG + chr2.4:4515889-4515908 MS.gene85228:CDS 40.0%
AGGCTAAGCTCAAAACTTAC+AGG + chr2.4:4517831-4517850 MS.gene85228:CDS 40.0%
AGTTATGCACTATATTCCCC+TGG - chr2.4:4516305-4516324 None:intergenic 40.0%
CAAGCTTAACAGAATCAGCA+TGG - chr2.4:4517106-4517125 None:intergenic 40.0%
CCCCAATAAAGGAAAAAAGG+GGG - chr2.4:4516234-4516253 None:intergenic 40.0%
GAGCTTAAAGTCGAAGATGA+TGG - chr2.4:4517686-4517705 None:intergenic 40.0%
GAGTTCAAAGAGACTCGTAA+TGG + chr2.4:4517865-4517884 MS.gene85228:CDS 40.0%
GGTCAATGACTTCAGTAGTT+AGG + chr2.4:4516644-4516663 MS.gene85228:intron 40.0%
TCTTCAACCATGAAGAAGAC+TGG + chr2.4:4515785-4515804 MS.gene85228:CDS 40.0%
TGCAAGCAATATTTAGAGGC+AGG + chr2.4:4516943-4516962 MS.gene85228:CDS 40.0%
TGGTTATGGTAATATGCCAG+GGG + chr2.4:4516286-4516305 MS.gene85228:intron 40.0%
TTAACTTCATCTGCAGTTCC+AGG - chr2.4:4517272-4517291 None:intergenic 40.0%
! TAGGTTCTTTTGTATGGCTG+TGG + chr2.4:4516672-4516691 MS.gene85228:intron 40.0%
! TTCCTAAGCTCTTGTTGTCA+TGG - chr2.4:4517511-4517530 None:intergenic 40.0%
!! AAGAGCTTTGAGAGCATTGA+GGG + chr2.4:4516908-4516927 MS.gene85228:CDS 40.0%
!! GTGGTGTTACTAATCTGTCA+GGG + chr2.4:4516691-4516710 MS.gene85228:intron 40.0%
!! TCTTTTTCCTCTGTTGCTTC+TGG - chr2.4:4517770-4517789 None:intergenic 40.0%
!! TGGTGTTACTAATCTGTCAG+GGG + chr2.4:4516692-4516711 MS.gene85228:intron 40.0%
!! TGTGGTGTTACTAATCTGTC+AGG + chr2.4:4516690-4516709 MS.gene85228:intron 40.0%
!!! CCCCCTTTTTTCCTTTATTG+GGG + chr2.4:4516231-4516250 MS.gene85228:intron 40.0%
ATCGTTGTGCAACACCTCTA+AGG + chr2.4:4517984-4518003 MS.gene85228:CDS 45.0%
ATCTATGCCAGAAGCAACAG+AGG + chr2.4:4517760-4517779 MS.gene85228:CDS 45.0%
CAAGGATGGTGTGAAATTCC+TGG + chr2.4:4517251-4517270 MS.gene85228:CDS 45.0%
CATCAAGAGGGATAGAGCAA+TGG + chr2.4:4517322-4517341 MS.gene85228:CDS 45.0%
CATCCTTATCAGGCAGGAAT+GGG + chr2.4:4515895-4515914 MS.gene85228:CDS 45.0%
CATTGCTCTATCCCTCTTGA+TGG - chr2.4:4517324-4517343 None:intergenic 45.0%
GAAGGAATGGTGTGACAACT+AGG + chr2.4:4517630-4517649 MS.gene85228:CDS 45.0%
GCAGAAATATCAGCCACTCT+TGG + chr2.4:4516778-4516797 MS.gene85228:intron 45.0%
GGCAAACAAACCATGTCCAT+GGG + chr2.4:4517553-4517572 MS.gene85228:CDS 45.0%
TCACTCTTCCTGCTTGAGAT+TGG - chr2.4:4517584-4517603 None:intergenic 45.0%
TCATCCTTATCAGGCAGGAA+TGG + chr2.4:4515894-4515913 MS.gene85228:CDS 45.0%
TCGTTGTGCAACACCTCTAA+GGG + chr2.4:4517985-4518004 MS.gene85228:CDS 45.0%
TGGCAAACAAACCATGTCCA+TGG + chr2.4:4517552-4517571 MS.gene85228:CDS 45.0%
! GTGTATGCAAGCTCTGGTTA+GGG + chr2.4:4516998-4517017 MS.gene85228:CDS 45.0%
! TCTAAGGTGTATGCAAGCTC+TGG + chr2.4:4516992-4517011 MS.gene85228:CDS 45.0%
!! GAAATAGCTTGTTAGAGCGC+TGG + chr2.4:4517528-4517547 MS.gene85228:CDS 45.0%
!! GAAGAGCTTTGAGAGCATTG+AGG + chr2.4:4516907-4516926 MS.gene85228:CDS 45.0%
!! TCTCTTTTTGGATTGGGGGA+GGG + chr2.4:4516622-4516641 MS.gene85228:intron 45.0%
!! TTCTCTTTTTGGATTGGGGG+AGG + chr2.4:4516621-4516640 MS.gene85228:intron 45.0%
AGCAGCACCAGTCTTCTTCA+TGG - chr2.4:4515795-4515814 None:intergenic 50.0%
AGCTCGAACAACGACAGCAA+CGG - chr2.4:4515861-4515880 None:intergenic 50.0%
ATTGGACTCTCCCATGGACA+TGG - chr2.4:4517566-4517585 None:intergenic 50.0%
CAGAATCAGCATGGTTGCGA+TGG - chr2.4:4517097-4517116 None:intergenic 50.0%
CTTGAGATTGGACTCTCCCA+TGG - chr2.4:4517572-4517591 None:intergenic 50.0%
GGCATACTCTCTATCCACAC+AGG + chr2.4:4517343-4517362 MS.gene85228:CDS 50.0%
GTGTGAGAGCTAGGAATGTG+AGG + chr2.4:4517030-4517049 MS.gene85228:CDS 50.0%
! ATGGGCTGCTATTCGCATTC+AGG + chr2.4:4515913-4515932 MS.gene85228:CDS 50.0%
! GGTGTATGCAAGCTCTGGTT+AGG + chr2.4:4516997-4517016 MS.gene85228:CDS 50.0%
!! CTCTTTTTGGATTGGGGGAG+GGG + chr2.4:4516623-4516642 MS.gene85228:intron 50.0%
!! GCAAGAAGGTGCCATCAAGA+GGG + chr2.4:4517310-4517329 MS.gene85228:CDS 50.0%
!! TAGCTTGTTAGAGCGCTGGA+TGG + chr2.4:4517532-4517551 MS.gene85228:CDS 50.0%
!! TTTGAGAGCATTGAGGGCAG+TGG + chr2.4:4516914-4516933 MS.gene85228:CDS 50.0%
AGCAACGGCAGCAGCAGTAT+CGG - chr2.4:4515846-4515865 None:intergenic 55.0%
CAGCCCATTCCTGCCTGATA+AGG - chr2.4:4515901-4515920 None:intergenic 55.0%
GGCTCTCTTTCGAGCCTTCT+TGG + chr2.4:4515934-4515953 MS.gene85228:CDS 55.0%
GGGAGAGTCCAATCTCAAGC+AGG + chr2.4:4517573-4517592 MS.gene85228:CDS 55.0%
GTTGCTGTCGTTGTTCGAGC+TGG + chr2.4:4515860-4515879 MS.gene85228:CDS 55.0%
TGCTGCACTGCTTTGCCTTC+TGG - chr2.4:4517062-4517081 None:intergenic 55.0%
TTCAGGCACGTGTGAGAGCT+AGG + chr2.4:4517021-4517040 MS.gene85228:CDS 55.0%
! GCAAGCTCTGGTTAGGGTTC+AGG + chr2.4:4517004-4517023 MS.gene85228:CDS 55.0%
!! GGCAAGAAGGTGCCATCAAG+AGG + chr2.4:4517309-4517328 MS.gene85228:CDS 55.0%
!! GGCAGCAGCAGTATCGGTAA+TGG - chr2.4:4515840-4515859 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4515582 4518042 4515582 ID=MS.gene85228
chr2.4 mRNA 4515582 4518042 4515582 ID=MS.gene85228.t1;Parent=MS.gene85228
chr2.4 exon 4515582 4515638 4515582 ID=MS.gene85228.t1.exon1;Parent=MS.gene85228.t1
chr2.4 CDS 4515582 4515638 4515582 ID=cds.MS.gene85228.t1;Parent=MS.gene85228.t1
chr2.4 exon 4515725 4515955 4515725 ID=MS.gene85228.t1.exon2;Parent=MS.gene85228.t1
chr2.4 CDS 4515725 4515955 4515725 ID=cds.MS.gene85228.t1;Parent=MS.gene85228.t1
chr2.4 exon 4516903 4517130 4516903 ID=MS.gene85228.t1.exon3;Parent=MS.gene85228.t1
chr2.4 CDS 4516903 4517130 4516903 ID=cds.MS.gene85228.t1;Parent=MS.gene85228.t1
chr2.4 exon 4517251 4517364 4517251 ID=MS.gene85228.t1.exon4;Parent=MS.gene85228.t1
chr2.4 CDS 4517251 4517364 4517251 ID=cds.MS.gene85228.t1;Parent=MS.gene85228.t1
chr2.4 exon 4517440 4518042 4517440 ID=MS.gene85228.t1.exon5;Parent=MS.gene85228.t1
chr2.4 CDS 4517440 4518042 4517440 ID=cds.MS.gene85228.t1;Parent=MS.gene85228.t1
Gene Sequence

>MS.gene85228

ATGGGTGTTTCTGGAAAATGGTTTAAATCACTTCTTTCCAATAAAAAAACCGATCAGGTAAATTAACAATCACTTCTTTTATTTATTTTTTTTCCTTTTTTTGTTGTTGTGTTAATAATTTAGTGTTAATGTTGTTTGTTCAGGAGAAGAAGTGTAATAGTACTAAGAAGAAATGGAAGCTATGGAAAACTTCTTCTTCTTCTTCTTCAACCATGAAGAAGACTGGTGCTGCTGCTGCTGCTGTGTCTGATTCTTCCATTACCGATACTGCTGCTGCCGTTGCTGTCGTTGTTCGAGCTGGACCTAAAGATTTCATCCTTATCAGGCAGGAATGGGCTGCTATTCGCATTCAGGCTCTCTTTCGAGCCTTCTTGGTAACTATTTCTTTTCCTTTCTATGATTTTTGCTGTGGCTTTTAATTGTTCATGTCATAGTGTGCTAGTTTGAAATTCCAAGTCCAATTATGGATCAGAATTCAGGGGTTACGATCTATTAAGTTTTTACAAGATCTACTAATAGTTGGGATTTGGGAGTTTAAAAAATGGACCTTTGTGTTTTCATGATTTGAATTTTGAGATCAACCTTCCTCAAAAAAATTGAATTTGAATTTTCAATTTTTTAGTCCTCTTTTAAGTCATGTAAACTTTTTCCCCCTTTTTTCCTTTATTGGGGTTGAATGATTTAGGTAGTTAGGTTCTTTTGTATGGTTATGGTAATATGCCAGGGGAATATAGTGCATAACTGAGTTATAAACACTAGCAAATGTTACCAAATGCATAACTGAGTTATAAACACTAGCAAATGTTACCAAAAATTGCAGAAATATTAGCCACTCTTTTTCACATTGTTTTAAGAGAATTTGATCATTATATTTTGCATATGTCACTTGTGCTTAGATTTTTCTATGCTAATGTTCTTCATGTTAACACTATCATATTTTGAATTGTGAAATCAGCTCGCAGCTCGCCTTTACCTAATTGAAATTCAACTTTTTAGTCCTCCTTAAGATGACTGTAAAATCCTCCCCTTTATTTTTTCTTTCTCTTTTTGGATTGGGGGAGGGGTCAATGACTTCAGTAGTTAGGTAACTTAGGTTCTTTTGTATGGCTGTGGTGTTACTAATCTGTCAGGGGAATATGATGCATAACTGAGTCTATCATTCAGCATAAACACTGCCCATTTTTTTAGGATTTATTGCAGAAATATCAGCCACTCTTGGTTACTTTTTTTTTTCTTCTTTTTCTATGTTGATACTCTTCTTTCATTGATTTAGTTATTTTATATTGATCTTACGTCTATTTCTTTTTAAAATACATTGAAGGCTAGAAGAGCTTTGAGAGCATTGAGGGCAGTGGTGAGACTGCAAGCAATATTTAGAGGCAGGCAAGTTCGAAAACAAGCAGCTGTTACTCTAAGGTGTATGCAAGCTCTGGTTAGGGTTCAGGCACGTGTGAGAGCTAGGAATGTGAGGAAATCTCCAGAAGGCAAAGCAGTGCAGCAGCTATTAGATGACCATCGCAACCATGCTGATTCTGTTAAGCTTGTAGAGGTAGCAAATTAATTTGTTTCAAGATAATAATTACATAATATTCTCTAAATGGTTATTTGGAGTTACAAGGTTATAGTATATGCTAACATAAGGGATAATTTGCCATGTTATTATTATCAGCAAGGATGGTGTGAAATTCCTGGAACTGCAGATGAAGTTAAAGCAAAGCTAAGAATGAGGCAAGAAGGTGCCATCAAGAGGGATAGAGCAATGGCATACTCTCTATCCACACAGGTAATTAAGTGTTGACAATATCTATTCATTTAGTTTAGTTTCAATTCCAATATATCTTTGCTTGCTTTTTAACAGTCAAGAATAAGTGCTAGTCCAAACTCAAAATCCACAAAATCAGTGTCTCTTTTCAAGCATCATCACAACCATGACAACAAGAGCTTAGGAAATAGCTTGTTAGAGCGCTGGATGGCAAACAAACCATGTCCATGGGAGAGTCCAATCTCAAGCAGGAAGAGTGAAGAACTTGTTCCTACTTTTCAATCAAGAAGGAATGGTGTGACAACTAGGATTTCAGCTCTTAAAATCTGTCAGCAAACTCCATCATCTTCGACTTTAAGCTCGGAATACATGAATGATGATAGCATTTTATCAACTTCATGTACATCTGGATTAACATCTATGCCAGAAGCAACAGAGGAAAAAGATGCTCACCTACCTAGCTACATGAATTTGACAGAATCAACTAAGGCTAAGCTCAAAACTTACAGGTCTTCTTCGCAGAGTTCAAAGAGACTCGTAATGGATGATTGTATGTCTCATAACACAAGTACAACTTTTCTGAATGGAGATAATAGTATAAACAGTTCTGGTTCTGACCCTTCAGTTAATTTATGGAAGGATCGTTGTGCAACACCTCTAAGGGCAAGTTATTATCAAAATCGATATGGCAGGAGATAG
Protein sequence

>MS.gene85228.t1

MGVSGKWFKSLLSNKKTDQEKKCNSTKKKWKLWKTSSSSSSTMKKTGAAAAAVSDSSITDTAAAVAVVVRAGPKDFILIRQEWAAIRIQALFRAFLARRALRALRAVVRLQAIFRGRQVRKQAAVTLRCMQALVRVQARVRARNVRKSPEGKAVQQLLDDHRNHADSVKLVEQGWCEIPGTADEVKAKLRMRQEGAIKRDRAMAYSLSTQSRISASPNSKSTKSVSLFKHHHNHDNKSLGNSLLERWMANKPCPWESPISSRKSEELVPTFQSRRNGVTTRISALKICQQTPSSSTLSSEYMNDDSILSTSCTSGLTSMPEATEEKDAHLPSYMNLTESTKAKLKTYRSSSQSSKRLVMDDCMSHNTSTTFLNGDNSINSSGSDPSVNLWKDRCATPLRASYYQNRYGRR