Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85694.t1 | XP_028762943.1 | 63.8 | 94 | 28 | 1 | 12 | 99 | 255 | 348 | 2.70E-26 | 127.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85694.t1 | A0A392P9H0 | 64.4 | 90 | 31 | 1 | 2 | 91 | 107 | 195 | 4.7e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene85694 | 0.817888 | 2.61E-52 | -1.69E-46 |
| MS.gene050026 | MS.gene85694 | 0.81246 | 4.19E-51 | -1.69E-46 |
| MS.gene050221 | MS.gene85694 | 0.805896 | 1.07E-49 | -1.69E-46 |
| MS.gene053512 | MS.gene85694 | 0.829614 | 4.67E-55 | -1.69E-46 |
| MS.gene056174 | MS.gene85694 | 0.801896 | 7.25E-49 | -1.69E-46 |
| MS.gene056854 | MS.gene85694 | 0.827007 | 1.98E-54 | -1.69E-46 |
| MS.gene058515 | MS.gene85694 | 0.817479 | 3.23E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene85694 | 0.862348 | 5.40E-64 | -1.69E-46 |
| MS.gene059276 | MS.gene85694 | 0.838929 | 2.15E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85694.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 19 | 99 | 5 | 80 | 2.34e-25 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.1:+17459452 | MS.gene85694:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.1:+17459444 | MS.gene85694:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.1:-17459606 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 6.1:-17459421 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 6.1:+17459504 | MS.gene85694:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 6.1:+17459496 | MS.gene85694:CDS |
| AATGCTGTAAAGAATGATAT+TGG | 0.409907 | 6.1:+17459322 | MS.gene85694:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 6.1:+17459373 | MS.gene85694:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 6.1:-17459534 | None:intergenic |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.1:+17459453 | MS.gene85694:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 6.1:+17459380 | MS.gene85694:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.1:+17459588 | MS.gene85694:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.1:+17459364 | MS.gene85694:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 6.1:+17459367 | MS.gene85694:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 6.1:-17459533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTCAAAATAAGGATAAAA+GGG | + | chr6.1:17459453-17459472 | MS.gene85694:CDS | 20.0% |
| ! | AATGCTGTAAAGAATGATAT+TGG | + | chr6.1:17459322-17459341 | MS.gene85694:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | + | chr6.1:17459452-17459471 | MS.gene85694:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | + | chr6.1:17459496-17459515 | MS.gene85694:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | + | chr6.1:17459504-17459523 | MS.gene85694:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | - | chr6.1:17459424-17459443 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | - | chr6.1:17459585-17459604 | None:intergenic | 25.0% |
| CATTAGAGATGAAAATATCC+AGG | + | chr6.1:17459588-17459607 | MS.gene85694:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | + | chr6.1:17459444-17459463 | MS.gene85694:CDS | 30.0% | |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | - | chr6.1:17459447-17459466 | None:intergenic | 30.0% |
| ! | AGATGGTCAATCTTTTCATC+AGG | - | chr6.1:17459568-17459587 | None:intergenic | 35.0% |
| AGAAATCAGCAGCATCTAAG+TGG | - | chr6.1:17459537-17459556 | None:intergenic | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | - | chr6.1:17459536-17459555 | None:intergenic | 40.0% | |
| !! | AGTATTTGCTATCGTTGTGG+AGG | + | chr6.1:17459367-17459386 | MS.gene85694:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | + | chr6.1:17459364-17459383 | MS.gene85694:CDS | 40.0% |
| GTTGTGGAGGAAAAGGTCAT+TGG | + | chr6.1:17459380-17459399 | MS.gene85694:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | + | chr6.1:17459373-17459392 | MS.gene85694:CDS | 45.0% | |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | - | chr6.1:17459413-17459432 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 17459316 | 17459615 | 17459316 | ID=MS.gene85694 |
| chr6.1 | mRNA | 17459316 | 17459615 | 17459316 | ID=MS.gene85694.t1;Parent=MS.gene85694 |
| chr6.1 | exon | 17459316 | 17459615 | 17459316 | ID=MS.gene85694.t1.exon1;Parent=MS.gene85694.t1 |
| chr6.1 | CDS | 17459316 | 17459615 | 17459316 | ID=cds.MS.gene85694.t1;Parent=MS.gene85694.t1 |
| Gene Sequence |
| Protein sequence |
