Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85767.t1 | XP_013467320.1 | 99.6 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 9.20E-140 | 506.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85767.t1 | Q03441 | 22.5 | 222 | 163 | 2 | 31 | 247 | 205 | 422 | 6.9e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85767.t1 | A0A072VHW2 | 99.6 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 6.6e-140 | 506.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050642 | MS.gene85767 | 0.833567 | 4.95E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene85767 | 0.836374 | 9.72E-57 | -1.69E-46 |
| MS.gene054930 | MS.gene85767 | 0.805432 | 1.34E-49 | -1.69E-46 |
| MS.gene055367 | MS.gene85767 | 0.81585 | 7.48E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene85767 | 0.805424 | 1.34E-49 | -1.69E-46 |
| MS.gene056087 | MS.gene85767 | 0.816876 | 4.41E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene85767 | 0.809597 | 1.75E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene85767 | 0.834772 | 2.47E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene85767 | 0.830034 | 3.69E-55 | -1.69E-46 |
| MS.gene061114 | MS.gene85767 | -0.834872 | 2.33E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85767.t1 | MTR_1g049180 | 99.606 | 254 | 1 | 0 | 1 | 254 | 153 | 406 | 0.0 | 525 |
| MS.gene85767.t1 | MTR_5g043180 | 66.800 | 250 | 81 | 2 | 2 | 250 | 160 | 408 | 4.96e-116 | 338 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85767.t1 | AT5G13610 | 75.600 | 250 | 61 | 0 | 1 | 250 | 152 | 401 | 1.10e-137 | 393 |
| MS.gene85767.t1 | AT1G69380 | 61.753 | 251 | 95 | 1 | 2 | 251 | 119 | 369 | 1.16e-108 | 318 |
Find 49 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCATCCTTTCTCATTTC+AGG | 0.181892 | 1.2:-32723647 | None:intergenic |
| CTCCCATATCTGACCATATT+TGG | 0.221053 | 1.2:-32730737 | None:intergenic |
| CCTGGTTCGTCTTTCTTGAA+TGG | 0.298986 | 1.2:+32723506 | MS.gene85767:CDS |
| AATTATGTAGTTCTCAAGTT+TGG | 0.333870 | 1.2:+32722563 | MS.gene85767:CDS |
| GTTGATGGAATTCGAACTAT+TGG | 0.339072 | 1.2:+32728761 | MS.gene85767:CDS |
| TACAAGAAATTCTTCAAAAC+AGG | 0.343403 | 1.2:+32730956 | MS.gene85767:CDS |
| ATGCCAAATATGGTCAGATA+TGG | 0.351572 | 1.2:+32730734 | MS.gene85767:CDS |
| AGTATTGCTCTTGATTACTA+TGG | 0.353838 | 1.2:+32728800 | MS.gene85767:CDS |
| CCACCTGTATTCTAGGTTGA+TGG | 0.358362 | 1.2:+32728910 | MS.gene85767:intron |
| ATGGCTATTTGAAAATTGTT+AGG | 0.362493 | 1.2:+32723603 | MS.gene85767:CDS |
| GTTCTCAAGTTTGGCAATCT+TGG | 0.366939 | 1.2:+32722572 | MS.gene85767:CDS |
| CGAACTATTGGTAGTGTACT+AGG | 0.382786 | 1.2:+32728773 | MS.gene85767:CDS |
| GTCAGATATGGGAGTATCTC+AGG | 0.391860 | 1.2:+32730746 | MS.gene85767:CDS |
| TGCATCCATGTGCTCAAAGC+TGG | 0.404184 | 1.2:-32728701 | None:intergenic |
| TTACATAATGTTACAATACT+TGG | 0.404564 | 1.2:+32728736 | MS.gene85767:CDS |
| GCTTGGAAAGATGCCAAATA+TGG | 0.412109 | 1.2:+32730724 | MS.gene85767:CDS |
| TGGCTCATTATTGCATTGAT+TGG | 0.416590 | 1.2:+32730997 | MS.gene85767:CDS |
| TTACAATACTTGGATGTTGA+TGG | 0.418643 | 1.2:+32728746 | MS.gene85767:CDS |
| CACATGGATGCAAGGAGGAC+TGG | 0.427102 | 1.2:+32728712 | MS.gene85767:CDS |
| AATTTGCCTTGCCTACCAAC+TGG | 0.429171 | 1.2:-32729011 | None:intergenic |
| TGATTTCAAGTTGAAATTTG+TGG | 0.429980 | 1.2:+32730804 | MS.gene85767:CDS |
| ATTGTTAGGAAGCATGCATC+TGG | 0.438001 | 1.2:+32723617 | MS.gene85767:CDS |
| TCTTTCTTGAATGGAACAAA+TGG | 0.446687 | 1.2:+32723515 | MS.gene85767:CDS |
| TTTACAGATCTGATATCGCT+TGG | 0.475775 | 1.2:+32730707 | MS.gene85767:intron |
| AGCCATCAACCTCGTGTTCG+AGG | 0.494923 | 1.2:-32723586 | None:intergenic |
| GTCCTCGAACACGAGGTTGA+TGG | 0.497425 | 1.2:+32723584 | MS.gene85767:CDS |
| TTTGCTTCCTGAAATGAGAA+AGG | 0.519357 | 1.2:+32723640 | MS.gene85767:CDS |
| TGCCAAATATGGTCAGATAT+GGG | 0.539187 | 1.2:+32730735 | MS.gene85767:CDS |
| TTGCTCTTGATTACTATGGC+CGG | 0.547854 | 1.2:+32728804 | MS.gene85767:CDS |
| TGGCAATCTTGGCGATTCCA+AGG | 0.548774 | 1.2:+32722583 | MS.gene85767:CDS |
| CAGGAAAATTTAAGATGCAA+AGG | 0.558742 | 1.2:+32728977 | MS.gene85767:CDS |
| CTCTATATGACATTGTTCAA+AGG | 0.569742 | 1.2:+32731037 | MS.gene85767:CDS |
| GCTTTGAGCACATGGATGCA+AGG | 0.583404 | 1.2:+32728704 | MS.gene85767:CDS |
| TCTTGATTACTATGGCCGGC+AGG | 0.586842 | 1.2:+32728808 | MS.gene85767:CDS |
| AATCGTGAGATGGAAGCGAC+AGG | 0.591664 | 1.2:+32728958 | MS.gene85767:CDS |
| GGAAATCTGATTTCCTTGAG+TGG | 0.592677 | 1.2:+32730977 | MS.gene85767:CDS |
| ATGCAATAATGAGCCACTCA+AGG | 0.594797 | 1.2:-32730990 | None:intergenic |
| GCTGATGTGATTCTAAAGCT+CGG | 0.595619 | 1.2:+32729042 | MS.gene85767:CDS |
| TGTATTCTAGGTTGATGGAA+TGG | 0.596030 | 1.2:+32728915 | MS.gene85767:intron |
| TTTCAAGTTGAAATTTGTGG+AGG | 0.601208 | 1.2:+32730807 | MS.gene85767:CDS |
| CCATTCAAGAAAGACGAACC+AGG | 0.605038 | 1.2:-32723506 | None:intergenic |
| TACAGACATAAATCGTGAGA+TGG | 0.607409 | 1.2:+32728948 | MS.gene85767:CDS |
| TGGAACAAATGGCTGTTACA+TGG | 0.615782 | 1.2:+32723526 | MS.gene85767:CDS |
| CTTCCTGAAATGAGAAAGGA+TGG | 0.617434 | 1.2:+32723644 | MS.gene85767:CDS |
| TAAAGCTCGGACTATTTGAG+AGG | 0.622265 | 1.2:+32729055 | MS.gene85767:CDS |
| GAGGACATTAAACAATACAA+TGG | 0.634285 | 1.2:-32723567 | None:intergenic |
| GTTTAATGTCCTCGAACACG+AGG | 0.636672 | 1.2:+32723577 | MS.gene85767:CDS |
| TCAGATATGGGAGTATCTCA+GGG | 0.654645 | 1.2:+32730747 | MS.gene85767:CDS |
| TTGAGCACATGGATGCAAGG+AGG | 0.721239 | 1.2:+32728707 | MS.gene85767:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 32722557 | 32731077 | 32722557 | ID=MS.gene85767 |
| chr1.2 | mRNA | 32722557 | 32731077 | 32722557 | ID=MS.gene85767.t1;Parent=MS.gene85767 |
| chr1.2 | exon | 32722557 | 32722604 | 32722557 | ID=MS.gene85767.t1.exon1;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32722557 | 32722604 | 32722557 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| chr1.2 | exon | 32723503 | 32723665 | 32723503 | ID=MS.gene85767.t1.exon2;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32723503 | 32723665 | 32723503 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| chr1.2 | exon | 32728681 | 32728829 | 32728681 | ID=MS.gene85767.t1.exon3;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32728681 | 32728829 | 32728681 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| chr1.2 | exon | 32728925 | 32729076 | 32728925 | ID=MS.gene85767.t1.exon4;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32728925 | 32729076 | 32728925 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| chr1.2 | exon | 32730714 | 32730828 | 32730714 | ID=MS.gene85767.t1.exon5;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32730714 | 32730828 | 32730714 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| chr1.2 | exon | 32730940 | 32731077 | 32730940 | ID=MS.gene85767.t1.exon6;Parent=MS.gene85767.t1 |
| chr1.2 | CDS | 32730940 | 32731077 | 32730940 | ID=cds.MS.gene85767.t1;Parent=MS.gene85767.t1 |
| Gene Sequence |
| Protein sequence |
