AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85770.t1 MTR_1g049120 93.424 958 43 1 1 958 1 938 0.0 1819
MS.gene85770.t1 MTR_4g036445 59.492 590 177 8 73 604 33 618 0.0 668
MS.gene85770.t1 MTR_1g072780 54.626 681 243 15 303 958 355 994 0.0 655
MS.gene85770.t1 MTR_2g047912 62.611 452 105 10 494 932 272 672 2.54e-164 497
MS.gene85770.t1 MTR_2g047912 38.158 304 119 7 1 266 1 273 5.03e-35 143
MS.gene85770.t1 MTR_2g104120 30.673 639 354 16 310 929 258 826 2.00e-83 288
MS.gene85770.t1 MTR_1g066890 31.934 667 345 19 301 929 307 902 5.52e-83 288
MS.gene85770.t1 MTR_2g104980 54.369 103 46 1 310 412 233 334 1.55e-34 138
MS.gene85770.t1 MTR_6g005840 27.556 450 268 15 92 493 14 453 8.08e-27 118
MS.gene85770.t1 MTR_6g005840 27.924 419 243 16 114 493 13 411 2.43e-26 116
MS.gene85770.t1 MTR_6g005960 35.233 193 121 4 306 497 208 397 2.43e-25 112
MS.gene85770.t1 MTR_7g113180 32.432 185 109 4 310 485 87 264 6.61e-24 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85770.t1 AT3G09670 37.371 388 191 12 309 646 196 581 4.82e-62 225
MS.gene85770.t1 AT3G09670 37.371 388 191 12 309 646 196 581 4.82e-62 225
MS.gene85770.t1 AT5G02950 40.000 280 137 8 313 584 98 354 4.55e-55 202
MS.gene85770.t1 AT5G02950 53.846 78 36 0 850 927 546 623 7.44e-18 89.0
MS.gene85770.t1 AT5G02950 40.000 280 137 8 313 584 98 354 4.55e-55 202
MS.gene85770.t1 AT5G02950 53.846 78 36 0 850 927 546 623 7.44e-18 89.0
MS.gene85770.t1 AT3G54760 37.500 344 137 8 598 934 470 742 2.89e-53 200
MS.gene85770.t1 AT3G54760 37.500 344 137 8 598 934 501 773 5.04e-53 199
MS.gene85770.t1 AT5G40340 36.041 197 117 6 310 501 125 317 1.20e-25 114
MS.gene85770.t1 AT5G40340 36.041 197 117 6 310 501 125 317 1.44e-25 114
MS.gene85770.t1 AT5G27650 33.702 181 112 5 310 485 171 348 9.80e-24 108
MS.gene85770.t1 AT3G05430 33.520 179 115 4 310 485 133 310 3.51e-22 103
MS.gene85770.t1 AT3G05430 33.520 179 115 4 310 485 133 310 3.67e-22 103
MS.gene85770.t1 AT3G27860 28.333 300 178 14 304 593 55 327 2.51e-15 80.9
MS.gene85770.t1 AT3G27860 28.333 300 178 14 304 593 55 327 2.51e-15 80.9

Find 208 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATTTCGTTGCATATTT+TGG 0.018484 1.2:+32693603 MS.gene85770:CDS
CCTCCACAATGTTGTATTTC+TGG 0.135555 1.2:-32694038 None:intergenic
GCTAAGAAGTTCAACATATT+TGG 0.166046 1.2:+32695289 MS.gene85770:CDS
AACAAAATGTTTAAGCGTTT+TGG 0.179710 1.2:+32695169 MS.gene85770:CDS
GACCATATTAACTTCAAAAT+TGG 0.197232 1.2:-32695413 None:intergenic
TTCTCTCTCAGATATGGTTT+GGG 0.239508 1.2:+32693491 MS.gene85770:CDS
TCTTCAAATTCAAATGTTTC+AGG 0.243949 1.2:-32694788 None:intergenic
TTTGTTAAGGTTTGCTTCTA+AGG 0.244285 1.2:-32695152 None:intergenic
GTCTCAAGACGAGTCGAATA+TGG 0.258530 1.2:+32693753 MS.gene85770:CDS
GGTGATTCTGTTGGGGTAGT+TGG 0.264248 1.2:+32692554 MS.gene85770:CDS
AACAATCGGCGACTTCAATT+TGG 0.266628 1.2:+32692724 MS.gene85770:CDS
TACGGCTGGCTTTGATCGTT+TGG 0.305489 1.2:+32693953 MS.gene85770:CDS
TTTCTCTCTCAGATATGGTT+TGG 0.312975 1.2:+32693490 MS.gene85770:CDS
TGAGTTGGGTAACGTTGTTT+TGG 0.316781 1.2:+32692652 MS.gene85770:CDS
AACAATCGGTGACTTTAATA+TGG 0.317580 1.2:+32692793 MS.gene85770:CDS
AATCACACCGCGGAGATTAA+AGG 0.319150 1.2:+32693064 MS.gene85770:CDS
GTGACCCAGTTCCAACTATT+GGG 0.320240 1.2:-32694766 None:intergenic
GGTGACCCAGTTCCAACTAT+TGG 0.324538 1.2:-32694767 None:intergenic
GACACATCTGATTCATTATC+AGG 0.324654 1.2:-32694521 None:intergenic
CGATTAACTTGGGCTGGCTT+TGG 0.335599 1.2:-32695001 None:intergenic
CCCAAAATATGCAACGAAAT+AGG 0.337444 1.2:-32693604 None:intergenic
TCAGGTGAAACCAGATTATC+TGG 0.347288 1.2:-32694269 None:intergenic
GGTGACTTGGTAGCTGGCTT+TGG 0.349758 1.2:-32694980 None:intergenic
ATCATGTCATAGGTGTCTTT+AGG 0.355766 1.2:-32693795 None:intergenic
AGTATCCGGTTGAGGAATCT+AGG 0.358039 1.2:+32692585 MS.gene85770:CDS
TGGTTTGTGGTGATTCTGTT+GGG 0.360124 1.2:+32692546 MS.gene85770:CDS
GGCTTTGGTGACTTGGTAGC+TGG 0.361549 1.2:-32694986 None:intergenic
AAGATATGAGCGACACATAT+TGG 0.375633 1.2:+32694807 MS.gene85770:CDS
CCATCACCATCAGGAGAATC+TGG 0.383648 1.2:-32694239 None:intergenic
AGAAGTCACCCATGGTGGCC+TGG 0.392403 1.2:+32693522 MS.gene85770:CDS
CGGATACATTTGGTATTAAG+AGG 0.392876 1.2:+32694735 MS.gene85770:CDS
GGTGACTTAACAGCTGGCTT+TGG 0.393998 1.2:-32694959 None:intergenic
TTCCGTGATGGTTGTTGCAC+TGG 0.394785 1.2:-32694896 None:intergenic
AATCTCCGCGGTGTGATTCA+CGG 0.396914 1.2:-32693059 None:intergenic
AGAGATCGAAAACCATTTCC+TGG 0.398045 1.2:-32695390 None:intergenic
CCCAACTGATGAGGATGATC+AGG 0.400132 1.2:+32694547 MS.gene85770:CDS
CACATACCACAGACCCATCT+AGG 0.400940 1.2:-32692508 None:intergenic
TCACTGCGTCACCTTCTATA+AGG 0.403119 1.2:+32694447 MS.gene85770:CDS
TAGATGGGTCTGTGGTATGT+GGG 0.406634 1.2:+32692510 MS.gene85770:CDS
TTGGGCTGGCTTTGGTGACT+TGG 0.409137 1.2:-32694993 None:intergenic
TGTGAGCGGTGTTTCTGCTT+TGG 0.414830 1.2:+32692961 MS.gene85770:CDS
TCTGTTTCAGATTCCTTAAG+TGG 0.417444 1.2:-32695193 None:intergenic
CTGTAGTTGTGGCTGCTGAT+TGG 0.424377 1.2:+32694696 MS.gene85770:CDS
GGCTTTGGTGACTTAACAGC+TGG 0.426756 1.2:-32694965 None:intergenic
GAAGTCACCCATGGTGGCCT+GGG 0.429264 1.2:+32693523 MS.gene85770:CDS
ATTCTGTTGGGGTAGTTGGT+GGG 0.439002 1.2:+32692558 MS.gene85770:CDS
TCAAAGTCTCCATCTGCCTT+TGG 0.439286 1.2:-32694491 None:intergenic
ACAAAGTCATCGTTTAAAAT+CGG 0.439705 1.2:+32694395 MS.gene85770:CDS
GCAACGGTGCATGGCTCCCT+TGG 0.441735 1.2:-32693281 None:intergenic
GTGGTTTGTGGTGATTCTGT+TGG 0.443493 1.2:+32692545 MS.gene85770:CDS
CTAGATGGGTCTGTGGTATG+TGG 0.448465 1.2:+32692509 MS.gene85770:CDS
TTCCATCGCGCAACGGTGCA+TGG 0.449140 1.2:-32693290 None:intergenic
TGGTCGGACTCCAGATAATC+TGG 0.450902 1.2:+32694259 MS.gene85770:CDS
CAAGGAGTCAAATGGTGGTC+AGG 0.451976 1.2:+32693326 MS.gene85770:CDS
GTGAGAGATGAAGCAGTAGT+TGG 0.452544 1.2:+32692683 MS.gene85770:CDS
CTAGAACACTTCCTTATAGA+AGG 0.452692 1.2:-32694458 None:intergenic
GTTGGTGGTGAAACCCTAGA+TGG 0.452873 1.2:+32692494 MS.gene85770:CDS
GCTTTAGCAGGTAGTTCTGT+AGG 0.454456 1.2:+32692869 MS.gene85770:CDS
CACCTGAACATTCCAAGAAA+AGG 0.455137 1.2:+32694285 MS.gene85770:CDS
AGTTGGTGGGACCGAGTATC+CGG 0.455528 1.2:+32692571 MS.gene85770:CDS
GATTCTGTTGGGGTAGTTGG+TGG 0.455837 1.2:+32692557 MS.gene85770:CDS
TAGTTCAGAAATAGTTCATC+AGG 0.456384 1.2:+32693428 MS.gene85770:CDS
TTCTGTTCGTGCAGGTTCGT+CGG 0.457233 1.2:+32693191 MS.gene85770:intron
TGCACTGGTTTCTGTTGCGC+TGG 0.460959 1.2:-32694866 None:intergenic
GGCTTACGATTAACTTGGGC+TGG 0.461800 1.2:-32695007 None:intergenic
AATCTGGAGTCCCATTCATT+AGG 0.464940 1.2:-32694223 None:intergenic
TATAGGGCTTACGATTAACT+TGG 0.466075 1.2:-32695012 None:intergenic
TATCTTGTGATCTGCGGCCT+CGG 0.467878 1.2:-32692825 None:intergenic
AAAGCGTAAATATAAGTTCA+AGG 0.468189 1.2:+32694160 MS.gene85770:CDS
TATTGAGACTGAGGAAAACT+TGG 0.469804 1.2:+32693218 MS.gene85770:CDS
TTTGGTGACTTAGCAGAGAC+TGG 0.474173 1.2:-32694941 None:intergenic
TCTTAAAGACCACTCTTGCC+CGG 0.475118 1.2:-32695234 None:intergenic
CTCCATCTGCCTTTGGAGAT+CGG 0.475922 1.2:-32694484 None:intergenic
AATCGGTGATTGTATTCGTC+GGG 0.480709 1.2:+32694412 MS.gene85770:CDS
GAGTGCTATTAATTGTGCTT+TGG 0.482515 1.2:+32693725 MS.gene85770:CDS
ACAACCCTAGATTCCTCAAC+CGG 0.485275 1.2:-32692590 None:intergenic
TCTCAAGACGAGTCGAATAT+GGG 0.485338 1.2:+32693754 MS.gene85770:CDS
GCTGCCGAGGCCGCAGTAGT+TGG 0.486116 1.2:+32692752 MS.gene85770:CDS
TCCTGATCATCCTCATCAGT+TGG 0.488623 1.2:-32694548 None:intergenic
GTTGTAGAATTTGAAGAAGC+CGG 0.488694 1.2:+32692608 MS.gene85770:CDS
GCTATCTTCACTTCAGCTTG+TGG 0.491218 1.2:+32694601 MS.gene85770:CDS
GAAGCTTTGAACAGTGCACT+TGG 0.493568 1.2:-32695346 None:intergenic
ACCACCAACTTTCACACCAT+GGG 0.493614 1.2:-32692480 None:intergenic
CATTTCGGAGTCATCGGCGT+AGG 0.493727 1.2:-32694327 None:intergenic
TTGTTAAGGTTTGCTTCTAA+GGG 0.496576 1.2:-32695151 None:intergenic
AGGATGTCAGACCTAATGAA+TGG 0.497931 1.2:+32694212 MS.gene85770:CDS
TGCAACACAAGACCAGGAAA+TGG 0.498131 1.2:+32695378 MS.gene85770:CDS
GGAGTCCGACCATCACCATC+AGG 0.498959 1.2:-32694248 None:intergenic
AATCGGCGACTTCAATTTGG+TGG 0.502290 1.2:+32692727 MS.gene85770:CDS
GAATGCGGCGGCAGTTGAGT+TGG 0.502816 1.2:+32692637 MS.gene85770:CDS
CTTTAGCAGGTAGTTCTGTA+GGG 0.504598 1.2:+32692870 MS.gene85770:CDS
AATGCGGCGGCAGTTGAGTT+GGG 0.505664 1.2:+32692638 MS.gene85770:CDS
ATCTTGCATTTCGGAGTCAT+CGG 0.509912 1.2:-32694333 None:intergenic
TGCACCGTTGCGCGATGGAA+AGG 0.511662 1.2:+32693293 MS.gene85770:CDS
AGATGGGTCTGTGGTATGTG+GGG 0.514040 1.2:+32692511 MS.gene85770:CDS
CACTGTGCATCCATCAAAGA+AGG 0.517034 1.2:+32694184 MS.gene85770:CDS
GGTCATAATGAAGTGGTTTG+TGG 0.517248 1.2:+32692533 MS.gene85770:CDS
ATTCCTATGCTCTTCAATTG+TGG 0.519415 1.2:-32693685 None:intergenic
TTGTAGAATTTGAAGAAGCC+GGG 0.519547 1.2:+32692609 MS.gene85770:CDS
CACTTGGAGTGTAATTAAGC+TGG 0.521123 1.2:-32695330 None:intergenic
GCCGCAGTAGTTGGTAGTAC+AGG 0.522140 1.2:+32692761 MS.gene85770:CDS
TCAGAAAAGTATACTCTCCA+AGG 0.523646 1.2:-32694634 None:intergenic
GCGACACATATTGGACTGAC+AGG 0.524575 1.2:+32694816 MS.gene85770:CDS
CTATCCGAGTTTCCTACGGC+TGG 0.525255 1.2:+32693939 MS.gene85770:CDS
CCTGATCATCCTCATCAGTT+GGG 0.525465 1.2:-32694547 None:intergenic
CAATTTGGTGGATGCTGCCG+AGG 0.525640 1.2:+32692739 MS.gene85770:CDS
ACCAGTGATGATGCTTTAGC+AGG 0.525871 1.2:+32692857 MS.gene85770:CDS
AAGTTGATAGAGTAAGCAGC+CGG 0.526372 1.2:+32695215 MS.gene85770:CDS
AAAGTCAACAAGGAGTCAAA+TGG 0.528610 1.2:+32693318 MS.gene85770:CDS
ATTTCGGAGTCATCGGCGTA+GGG 0.529828 1.2:-32694326 None:intergenic
TTTCAGAAATTCTGTAGTTG+TGG 0.530415 1.2:+32694685 MS.gene85770:CDS
ACATATTCTATAAGATTCAA+AGG 0.532546 1.2:-32693909 None:intergenic
GGATGTCAGACCTAATGAAT+GGG 0.533475 1.2:+32694213 MS.gene85770:CDS
AGTAAGCAGCCGGGCAAGAG+TGG 0.535181 1.2:+32695225 MS.gene85770:CDS
AAACTAAGAGATAGAAAAGC+CGG 0.536173 1.2:+32693381 MS.gene85770:CDS
AGATTCCTCAACCGGATACT+CGG 0.539506 1.2:-32692582 None:intergenic
GACGGATCAAATATCTGCCC+AGG 0.544596 1.2:-32693540 None:intergenic
TTTGGAGCTTGTGACTGCTA+AGG 0.547748 1.2:+32693971 MS.gene85770:CDS
TTGGGGACAAAACATTTGCA+TGG 0.547879 1.2:+32693622 MS.gene85770:CDS
ATTTATTATCTTGTGATCTG+CGG 0.548054 1.2:-32692831 None:intergenic
GTCAGACAGTAGAGAACACC+GGG 0.548651 1.2:+32693823 MS.gene85770:CDS
AAGTTCATAACAAGTTCAGC+AGG 0.549434 1.2:-32695109 None:intergenic
AAACATGCACTTGTGAAGCC+TGG 0.550664 1.2:+32693405 MS.gene85770:CDS
TGCCGCCGCATTCTCACTCC+CGG 0.551666 1.2:-32692627 None:intergenic
TCTCTCTCAGATATGGTTTG+GGG 0.553140 1.2:+32693492 MS.gene85770:CDS
AGCCATGCACCGTTGCGCGA+TGG 0.555384 1.2:+32693288 MS.gene85770:CDS
AACACTATCCGAGTTTCCTA+CGG 0.556285 1.2:+32693935 MS.gene85770:CDS
CACCACCAACTTTCACACCA+TGG 0.558331 1.2:-32692481 None:intergenic
GCCCTCTCAGATGCATCTGA+CGG 0.559014 1.2:-32693558 None:intergenic
CCAGATTCTCCTGATGGTGA+TGG 0.559245 1.2:+32694239 MS.gene85770:CDS
AAATCGGTGATTGTATTCGT+CGG 0.559399 1.2:+32694411 MS.gene85770:CDS
ATGACTCCGAAATGCAAGAT+GGG 0.560146 1.2:+32694336 MS.gene85770:CDS
GACACTCAAGTTACTGACCA+AGG 0.560989 1.2:+32693264 MS.gene85770:CDS
TGGGACCGAGTATCCGGTTG+AGG 0.563199 1.2:+32692577 MS.gene85770:CDS
TGAACTATTTCTGAACTACC+AGG 0.564289 1.2:-32693423 None:intergenic
TGACCCAGTTCCAACTATTG+GGG 0.565841 1.2:-32694765 None:intergenic
ATATCTGCCCAGGCCACCAT+GGG 0.565877 1.2:-32693530 None:intergenic
GATGACTCCGAAATGCAAGA+TGG 0.571916 1.2:+32694335 MS.gene85770:CDS
CAGAAAAGTATACTCTCCAA+GGG 0.576757 1.2:-32694633 None:intergenic
ACTTATTTCTTGTTGAACCC+CGG 0.576846 1.2:-32693841 None:intergenic
GACCGATCTCCAAAGGCAGA+TGG 0.577314 1.2:+32694482 MS.gene85770:CDS
AAAGCCAGCCGTAGGAAACT+CGG 0.577817 1.2:-32693943 None:intergenic
AATAAGTATCACACATGGGG+TGG 0.580958 1.2:+32693857 MS.gene85770:CDS
CCTTGAAAAGTGCAACTCAA+AGG 0.582951 1.2:+32692916 MS.gene85770:CDS
ACAGTTATCGAAACAACAAT+CGG 0.583839 1.2:+32692710 MS.gene85770:CDS
TAATATGGTGGATGCTGCCG+AGG 0.584046 1.2:+32692808 MS.gene85770:CDS
GGGACTCCAGATTCTCCTGA+TGG 0.585166 1.2:+32694233 MS.gene85770:CDS
TTCTAGTGACCGATCTCCAA+AGG 0.585712 1.2:+32694475 MS.gene85770:CDS
GGTTTGTGGTGATTCTGTTG+GGG 0.587388 1.2:+32692547 MS.gene85770:CDS
ACAGGTGTCGAAACAACAAT+CGG 0.587845 1.2:+32692779 MS.gene85770:CDS
TGTTGATGCAACACAAGACC+AGG 0.588104 1.2:+32695372 MS.gene85770:CDS
AATATCTGCCCAGGCCACCA+TGG 0.588370 1.2:-32693531 None:intergenic
ACCTGCTAAAGCATCATCAC+TGG 0.593003 1.2:-32692858 None:intergenic
TTCTGAAAGCAATCCTGCCG+AGG 0.595525 1.2:+32695048 MS.gene85770:CDS
TCTCCACAATTGAAGAGCAT+AGG 0.596184 1.2:+32693682 MS.gene85770:CDS
ACTACCAACTACTGCGGCCT+CGG 0.596716 1.2:-32692756 None:intergenic
GTGACCCATGGTGTGAAAGT+TGG 0.597666 1.2:+32692476 MS.gene85770:CDS
GTATCCGGTTGAGGAATCTA+GGG 0.602732 1.2:+32692586 MS.gene85770:CDS
ACATACCACAGACCCATCTA+GGG 0.603316 1.2:-32692507 None:intergenic
AAGCACAATTAATAGCACTC+TGG 0.605736 1.2:-32693722 None:intergenic
CAAGAAATAAGTATCACACA+TGG 0.606499 1.2:+32693852 MS.gene85770:CDS
AAGAAATAAGTATCACACAT+GGG 0.606954 1.2:+32693853 MS.gene85770:CDS
ATAGGGCTTACGATTAACTT+GGG 0.608401 1.2:-32695011 None:intergenic
ACCCATGGTGTGAAAGTTGG+TGG 0.609319 1.2:+32692479 MS.gene85770:CDS
ACATTGTGGAGGTGTTGACC+CGG 0.610706 1.2:+32694049 MS.gene85770:CDS
ATTCTCCTGATGGTGATGGT+CGG 0.611694 1.2:+32694243 MS.gene85770:CDS
AATCGGTGACTTTAATATGG+TGG 0.613810 1.2:+32692796 MS.gene85770:CDS
TAAGGGAACAGAATTGAGCT+CGG 0.619176 1.2:-32695134 None:intergenic
TAAGAAATGTGTAGATGCAG+AGG 0.619624 1.2:+32695252 MS.gene85770:CDS
CTTCTTACCTTTAATCTCCG+CGG 0.620007 1.2:-32693071 None:intergenic
TCCGTCAGATGCATCTGAGA+GGG 0.624122 1.2:+32693557 MS.gene85770:CDS
AGTTGATAGAGTAAGCAGCC+GGG 0.624544 1.2:+32695216 MS.gene85770:CDS
AACCAGTGCAACAACCATCA+CGG 0.625880 1.2:+32694894 MS.gene85770:CDS
TGTGTCTCTCCCAACTGATG+AGG 0.631262 1.2:+32694538 MS.gene85770:CDS
AAACGATCAAAGCCAGCCGT+AGG 0.635310 1.2:-32693951 None:intergenic
TTGGTGGTGAAACCCTAGAT+GGG 0.636238 1.2:+32692495 MS.gene85770:CDS
GATGGGTCTGTGGTATGTGG+GGG 0.641914 1.2:+32692512 MS.gene85770:CDS
GCATTGTCATCTATATAACC+AGG 0.644206 1.2:-32695085 None:intergenic
CTTGTGGCACAAGAACCCCT+TGG 0.646167 1.2:+32694617 MS.gene85770:CDS
GTCGGTGTCTATTGAGACTG+AGG 0.648701 1.2:+32693209 MS.gene85770:CDS
ATCCGTCAGATGCATCTGAG+AGG 0.653593 1.2:+32693556 MS.gene85770:CDS
GACCCAGTTCCAACTATTGG+GGG 0.653992 1.2:-32694764 None:intergenic
GTCAACAAGGAGTCAAATGG+TGG 0.655524 1.2:+32693321 MS.gene85770:CDS
TTTAGCAGGTAGTTCTGTAG+GGG 0.655647 1.2:+32692871 MS.gene85770:CDS
CGTGAGGAGATGCAAATAGA+CGG 0.660655 1.2:+32693004 MS.gene85770:CDS
AGAAATAAGTATCACACATG+GGG 0.660741 1.2:+32693854 MS.gene85770:CDS
GCAAAGTGAGAAGTCACCCA+TGG 0.661126 1.2:+32693514 MS.gene85770:CDS
AGAAGCCGGGAGTGAGAATG+CGG 0.661274 1.2:+32692622 MS.gene85770:CDS
ACACTCAAGTTACTGACCAA+GGG 0.665022 1.2:+32693265 MS.gene85770:CDS
AGTCAGACAGTAGAGAACAC+CGG 0.667248 1.2:+32693822 MS.gene85770:CDS
CGACACATATTGGACTGACA+GGG 0.669789 1.2:+32694817 MS.gene85770:CDS
AGCCGGGAGTGAGAATGCGG+CGG 0.674067 1.2:+32692625 MS.gene85770:CDS
ACTGACAGGGTCATTGACAA+TGG 0.674175 1.2:+32694830 MS.gene85770:CDS
TCAGAAACCACTGTGACCCA+TGG 0.675916 1.2:+32692464 MS.gene85770:CDS
AAGTGAGAAGTCACCCATGG+TGG 0.677906 1.2:+32693517 MS.gene85770:CDS
TGAAACCCTAGATGGGTCTG+TGG 0.684006 1.2:+32692502 MS.gene85770:CDS
TCAACAATCTGATGTACGTG+AGG 0.685189 1.2:+32692988 MS.gene85770:CDS
TTTCACACCATGGGTCACAG+TGG 0.691236 1.2:-32692471 None:intergenic
TTGCCAGAAATACAACATTG+TGG 0.702327 1.2:+32694035 MS.gene85770:CDS
TTGTCATCTATATAACCAGG+AGG 0.713037 1.2:-32695082 None:intergenic
CCAGAAATACAACATTGTGG+AGG 0.718025 1.2:+32694038 MS.gene85770:CDS
TAGACGGTGAAGATCAACAA+GGG 0.719756 1.2:+32693020 MS.gene85770:CDS
AGAAAAGTATACTCTCCAAG+GGG 0.750501 1.2:-32694632 None:intergenic
ATAGCTGACAACGATGTGAG+CGG 0.753803 1.2:+32692947 MS.gene85770:CDS
ACCTGTACTACCAACTACTG+CGG 0.755652 1.2:-32692762 None:intergenic
ATAGACGGTGAAGATCAACA+AGG 0.790484 1.2:+32693019 MS.gene85770:CDS
TGAAACCGTGAATCACACCG+CGG 0.851006 1.2:+32693054 MS.gene85770:CDS
TCAGACAGTAGAGAACACCG+GGG 0.900900 1.2:+32693824 MS.gene85770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATTCTATAAGATTCAA+AGG - chr1.2:32693912-32693931 None:intergenic 20.0%
!! ATTGAAAAAGTTAATGAAGT+TGG + chr1.2:32693351-32693370 MS.gene85770:CDS 20.0%
!! TTGAAAAAGTTAATGAAGTT+GGG + chr1.2:32693352-32693371 MS.gene85770:CDS 20.0%
! AAAGCGTAAATATAAGTTCA+AGG + chr1.2:32694160-32694179 MS.gene85770:CDS 25.0%
! AAGAAATAAGTATCACACAT+GGG + chr1.2:32693853-32693872 MS.gene85770:CDS 25.0%
! ATTTATTATCTTGTGATCTG+CGG - chr1.2:32692834-32692853 None:intergenic 25.0%
! GACCATATTAACTTCAAAAT+TGG - chr1.2:32695416-32695435 None:intergenic 25.0%
! TCTTCAAATTCAAATGTTTC+AGG - chr1.2:32694791-32694810 None:intergenic 25.0%
!! AACAAAATGTTTAAGCGTTT+TGG + chr1.2:32695169-32695188 MS.gene85770:CDS 25.0%
!! AACGCTTAAACATTTTGTTA+AGG - chr1.2:32695168-32695187 None:intergenic 25.0%
!! ACAAAGTCATCGTTTAAAAT+CGG + chr1.2:32694395-32694414 MS.gene85770:CDS 25.0%
!! ACTTATATTTACGCTTTTGA+CGG - chr1.2:32694157-32694176 None:intergenic 25.0%
!!! AGAATTGTTTTTCTTGGTAT+AGG - chr1.2:32695032-32695051 None:intergenic 25.0%
!!! ATCTAAGCTAAAGCATTTTA+GGG + chr1.2:32693653-32693672 MS.gene85770:CDS 25.0%
!!! CTCCAATTTTGAAGTTAATA+TGG + chr1.2:32695411-32695430 MS.gene85770:CDS 25.0%
!!! CTTGCTTTTAATCGTTTTAA+GGG + chr1.2:32694002-32694021 MS.gene85770:CDS 25.0%
!!! GAATTGTTTTTCTTGGTATA+GGG - chr1.2:32695031-32695050 None:intergenic 25.0%
AAACTAAGAGATAGAAAAGC+CGG + chr1.2:32693381-32693400 MS.gene85770:CDS 30.0%
AACAATCGGTGACTTTAATA+TGG + chr1.2:32692793-32692812 MS.gene85770:CDS 30.0%
ACAGTTATCGAAACAACAAT+CGG + chr1.2:32692710-32692729 MS.gene85770:CDS 30.0%
AGAAATAAGTATCACACATG+GGG + chr1.2:32693854-32693873 MS.gene85770:CDS 30.0%
ATCCATCAAAGAAGGAAAAA+AGG + chr1.2:32694192-32694211 MS.gene85770:CDS 30.0%
CAAGAAATAAGTATCACACA+TGG + chr1.2:32693852-32693871 MS.gene85770:CDS 30.0%
CATCGTCAACAAAAAATGAA+TGG - chr1.2:32694076-32694095 None:intergenic 30.0%
GCTAAGAAGTTCAACATATT+TGG + chr1.2:32695289-32695308 MS.gene85770:CDS 30.0%
GGAAAAAACACCAATGAAAT+CGG - chr1.2:32693104-32693123 None:intergenic 30.0%
TAGTTCAGAAATAGTTCATC+AGG + chr1.2:32693428-32693447 MS.gene85770:CDS 30.0%
TCAACAAAAAATGAATGGTC+CGG - chr1.2:32694071-32694090 None:intergenic 30.0%
TTTCAGAAATTCTGTAGTTG+TGG + chr1.2:32694685-32694704 MS.gene85770:CDS 30.0%
! ATTTTACGTACGGATACATT+TGG + chr1.2:32694725-32694744 MS.gene85770:CDS 30.0%
! CCTATTTCGTTGCATATTTT+GGG + chr1.2:32693604-32693623 MS.gene85770:CDS 30.0%
! CTATTTCGTTGCATATTTTG+GGG + chr1.2:32693605-32693624 MS.gene85770:CDS 30.0%
! CTGACTTTTAATCATGTCAT+AGG - chr1.2:32693808-32693827 None:intergenic 30.0%
! CTTATATTTACGCTTTTGAC+GGG - chr1.2:32694156-32694175 None:intergenic 30.0%
! GATTTTAAACGATGACTTTG+TGG - chr1.2:32694396-32694415 None:intergenic 30.0%
! TATATAACCAGGAGGTTTTT+CGG - chr1.2:32695077-32695096 None:intergenic 30.0%
! TCCTATTTCGTTGCATATTT+TGG + chr1.2:32693603-32693622 MS.gene85770:CDS 30.0%
! TTGGAAAGAGATTTTACGTA+CGG + chr1.2:32694715-32694734 MS.gene85770:CDS 30.0%
! TTGTTAAGGTTTGCTTCTAA+GGG - chr1.2:32695154-32695173 None:intergenic 30.0%
! TTTGTTAAGGTTTGCTTCTA+AGG - chr1.2:32695155-32695174 None:intergenic 30.0%
!! CATCTAAGCTAAAGCATTTT+AGG + chr1.2:32693652-32693671 MS.gene85770:CDS 30.0%
!! GCTTGCTTTTAATCGTTTTA+AGG + chr1.2:32694001-32694020 MS.gene85770:CDS 30.0%
!! GCTTTCAGAATTGTTTTTCT+TGG - chr1.2:32695038-32695057 None:intergenic 30.0%
!!! ATCCTTTTTTCCTTCTTTGA+TGG - chr1.2:32694197-32694216 None:intergenic 30.0%
AAAGTCAACAAGGAGTCAAA+TGG + chr1.2:32693318-32693337 MS.gene85770:CDS 35.0%
AAATCGGTGATTGTATTCGT+CGG + chr1.2:32694411-32694430 MS.gene85770:CDS 35.0%
AAGATATGAGCGACACATAT+TGG + chr1.2:32694807-32694826 MS.gene85770:CDS 35.0%
AAGCACAATTAATAGCACTC+TGG - chr1.2:32693725-32693744 None:intergenic 35.0%
AAGTTCATAACAAGTTCAGC+AGG - chr1.2:32695112-32695131 None:intergenic 35.0%
AATCGGTGACTTTAATATGG+TGG + chr1.2:32692796-32692815 MS.gene85770:CDS 35.0%
ATAGGGCTTACGATTAACTT+GGG - chr1.2:32695014-32695033 None:intergenic 35.0%
ATCATGTCATAGGTGTCTTT+AGG - chr1.2:32693798-32693817 None:intergenic 35.0%
ATTCCTATGCTCTTCAATTG+TGG - chr1.2:32693688-32693707 None:intergenic 35.0%
CAACAAAAAATGAATGGTCC+GGG - chr1.2:32694070-32694089 None:intergenic 35.0%
CCCAAAATATGCAACGAAAT+AGG - chr1.2:32693607-32693626 None:intergenic 35.0%
CGGATACATTTGGTATTAAG+AGG + chr1.2:32694735-32694754 MS.gene85770:CDS 35.0%
CTAGAACACTTCCTTATAGA+AGG - chr1.2:32694461-32694480 None:intergenic 35.0%
CTGCCAAAAGAAAAAGAAGA+AGG + chr1.2:32693462-32693481 MS.gene85770:CDS 35.0%
GACACATCTGATTCATTATC+AGG - chr1.2:32694524-32694543 None:intergenic 35.0%
GCAACTCCTGTAGTTAAAAA+AGG + chr1.2:32694125-32694144 MS.gene85770:CDS 35.0%
GCATTGTCATCTATATAACC+AGG - chr1.2:32695088-32695107 None:intergenic 35.0%
GTTGTAGAATTTGAAGAAGC+CGG + chr1.2:32692608-32692627 MS.gene85770:CDS 35.0%
TAAGAAATGTGTAGATGCAG+AGG + chr1.2:32695252-32695271 MS.gene85770:CDS 35.0%
TATAGGGCTTACGATTAACT+TGG - chr1.2:32695015-32695034 None:intergenic 35.0%
TATTGAGACTGAGGAAAACT+TGG + chr1.2:32693218-32693237 MS.gene85770:CDS 35.0%
TCTGTTTCAGATTCCTTAAG+TGG - chr1.2:32695196-32695215 None:intergenic 35.0%
TGGAAAGGAAAAAGTCAACA+AGG + chr1.2:32693308-32693327 MS.gene85770:CDS 35.0%
TTCTCTCTCAGATATGGTTT+GGG + chr1.2:32693491-32693510 MS.gene85770:CDS 35.0%
TTGCCAGAAATACAACATTG+TGG + chr1.2:32694035-32694054 MS.gene85770:CDS 35.0%
TTGTAGAATTTGAAGAAGCC+GGG + chr1.2:32692609-32692628 MS.gene85770:CDS 35.0%
TTGTCATCTATATAACCAGG+AGG - chr1.2:32695085-32695104 None:intergenic 35.0%
TTTCTCTCTCAGATATGGTT+TGG + chr1.2:32693490-32693509 MS.gene85770:CDS 35.0%
! ACTTATTTCTTGTTGAACCC+CGG - chr1.2:32693844-32693863 None:intergenic 35.0%
! AGAAAAGTATACTCTCCAAG+GGG - chr1.2:32694635-32694654 None:intergenic 35.0%
! ATATAACCAGGAGGTTTTTC+GGG - chr1.2:32695076-32695095 None:intergenic 35.0%
! CAGAAAAGTATACTCTCCAA+GGG - chr1.2:32694636-32694655 None:intergenic 35.0%
! GAGTGCTATTAATTGTGCTT+TGG + chr1.2:32693725-32693744 MS.gene85770:CDS 35.0%
! TCAGAAAAGTATACTCTCCA+AGG - chr1.2:32694637-32694656 None:intergenic 35.0%
! TCGCCTTCTTCTTTTTCTTT+TGG - chr1.2:32693468-32693487 None:intergenic 35.0%
! TTTTCTCCCATCTTGCATTT+CGG - chr1.2:32694345-32694364 None:intergenic 35.0%
!! AAGATATCTGCCGATTTCAT+TGG + chr1.2:32693091-32693110 MS.gene85770:intron 35.0%
!! GGAGAGTATACTTTTCTGAA+TGG + chr1.2:32694638-32694657 MS.gene85770:CDS 35.0%
!! TGAACTATTTCTGAACTACC+AGG - chr1.2:32693426-32693445 None:intergenic 35.0%
!!! GTGGTTTCTGAAACATTTTC+TGG - chr1.2:32692455-32692474 None:intergenic 35.0%
!!! GTTTTTTCCAGCTTAACTAG+TGG + chr1.2:32693115-32693134 MS.gene85770:intron 35.0%
!!! TTTTTCTGGTTTTTCCGTGA+TGG - chr1.2:32694911-32694930 None:intergenic 35.0%
AACAATCGGCGACTTCAATT+TGG + chr1.2:32692724-32692743 MS.gene85770:CDS 40.0%
AACACTATCCGAGTTTCCTA+CGG + chr1.2:32693935-32693954 MS.gene85770:CDS 40.0%
AATAAGTATCACACATGGGG+TGG + chr1.2:32693857-32693876 MS.gene85770:CDS 40.0%
AATCGGTGATTGTATTCGTC+GGG + chr1.2:32694412-32694431 MS.gene85770:CDS 40.0%
ACACTCAAGTTACTGACCAA+GGG + chr1.2:32693265-32693284 MS.gene85770:CDS 40.0%
AGAGATCGAAAACCATTTCC+TGG - chr1.2:32695393-32695412 None:intergenic 40.0%
AGGATGTCAGACCTAATGAA+TGG + chr1.2:32694212-32694231 MS.gene85770:CDS 40.0%
ATAGACGGTGAAGATCAACA+AGG + chr1.2:32693019-32693038 MS.gene85770:CDS 40.0%
ATCTTGCATTTCGGAGTCAT+CGG - chr1.2:32694336-32694355 None:intergenic 40.0%
ATGACTCCGAAATGCAAGAT+GGG + chr1.2:32694336-32694355 MS.gene85770:CDS 40.0%
CAAGTAACCACTAGTTAAGC+TGG - chr1.2:32693125-32693144 None:intergenic 40.0%
CACCTGAACATTCCAAGAAA+AGG + chr1.2:32694285-32694304 MS.gene85770:CDS 40.0%
CACTTGGAGTGTAATTAAGC+TGG - chr1.2:32695333-32695352 None:intergenic 40.0%
CCAGAAATACAACATTGTGG+AGG + chr1.2:32694038-32694057 MS.gene85770:CDS 40.0%
CCTCCACAATGTTGTATTTC+TGG - chr1.2:32694041-32694060 None:intergenic 40.0%
CTTCTTACCTTTAATCTCCG+CGG - chr1.2:32693074-32693093 None:intergenic 40.0%
GGATGTCAGACCTAATGAAT+GGG + chr1.2:32694213-32694232 MS.gene85770:CDS 40.0%
GGTCATAATGAAGTGGTTTG+TGG + chr1.2:32692533-32692552 MS.gene85770:CDS 40.0%
TAAGGGAACAGAATTGAGCT+CGG - chr1.2:32695137-32695156 None:intergenic 40.0%
TAGACGGTGAAGATCAACAA+GGG + chr1.2:32693020-32693039 MS.gene85770:CDS 40.0%
TCAACAATCTGATGTACGTG+AGG + chr1.2:32692988-32693007 MS.gene85770:CDS 40.0%
TCAGGTGAAACCAGATTATC+TGG - chr1.2:32694272-32694291 None:intergenic 40.0%
TCTCAAGACGAGTCGAATAT+GGG + chr1.2:32693754-32693773 MS.gene85770:CDS 40.0%
TCTCTCTCAGATATGGTTTG+GGG + chr1.2:32693492-32693511 MS.gene85770:CDS 40.0%
TTGGGGACAAAACATTTGCA+TGG + chr1.2:32693622-32693641 MS.gene85770:CDS 40.0%
! AATCTGGAGTCCCATTCATT+AGG - chr1.2:32694226-32694245 None:intergenic 40.0%
! CCTTGAAAAGTGCAACTCAA+AGG + chr1.2:32692916-32692935 MS.gene85770:CDS 40.0%
! CGAGTTTTCTCTCTCAGATA+TGG + chr1.2:32693485-32693504 MS.gene85770:CDS 40.0%
! CTCCTTTTCTTGGAATGTTC+AGG - chr1.2:32694290-32694309 None:intergenic 40.0%
! CTTCACAAGTGCATGTTTTC+CGG - chr1.2:32693403-32693422 None:intergenic 40.0%
! CTTTAGCAGGTAGTTCTGTA+GGG + chr1.2:32692870-32692889 MS.gene85770:CDS 40.0%
! TCTCCACAATTGAAGAGCAT+AGG + chr1.2:32693682-32693701 MS.gene85770:CDS 40.0%
! TGGTTTGTGGTGATTCTGTT+GGG + chr1.2:32692546-32692565 MS.gene85770:CDS 40.0%
! TTTAGCAGGTAGTTCTGTAG+GGG + chr1.2:32692871-32692890 MS.gene85770:CDS 40.0%
!! AAGTTGATAGAGTAAGCAGC+CGG + chr1.2:32695215-32695234 MS.gene85770:CDS 40.0%
!! ACAGGTGTCGAAACAACAAT+CGG + chr1.2:32692779-32692798 MS.gene85770:CDS 40.0%
!! GAGTTGGGTAACGTTGTTTT+GGG + chr1.2:32692653-32692672 MS.gene85770:CDS 40.0%
!! TAAGCGTTTTGGACCACTTA+AGG + chr1.2:32695180-32695199 MS.gene85770:CDS 40.0%
!! TGAGTTGGGTAACGTTGTTT+TGG + chr1.2:32692652-32692671 MS.gene85770:CDS 40.0%
!!! CCTTTGAGTTGCACTTTTCA+AGG - chr1.2:32692919-32692938 None:intergenic 40.0%
!!! CTGGTTGTTCGTCTTTTTTC+TGG - chr1.2:32694925-32694944 None:intergenic 40.0%
!!! GTGCTGCCTTTTTTAACTAC+AGG - chr1.2:32694134-32694153 None:intergenic 40.0%
AAACATGCACTTGTGAAGCC+TGG + chr1.2:32693405-32693424 MS.gene85770:CDS 45.0%
AAACCCCCAATAGTTGGAAC+TGG + chr1.2:32694761-32694780 MS.gene85770:CDS 45.0%
AACCAGTGCAACAACCATCA+CGG + chr1.2:32694894-32694913 MS.gene85770:CDS 45.0%
AACCCCCAATAGTTGGAACT+GGG + chr1.2:32694762-32694781 MS.gene85770:CDS 45.0%
AATCACACCGCGGAGATTAA+AGG + chr1.2:32693064-32693083 MS.gene85770:CDS 45.0%
AATCGGCGACTTCAATTTGG+TGG + chr1.2:32692727-32692746 MS.gene85770:CDS 45.0%
ACAACCCTAGATTCCTCAAC+CGG - chr1.2:32692593-32692612 None:intergenic 45.0%
ACATACCACAGACCCATCTA+GGG - chr1.2:32692510-32692529 None:intergenic 45.0%
ACCACCAACTTTCACACCAT+GGG - chr1.2:32692483-32692502 None:intergenic 45.0%
ACCTGTACTACCAACTACTG+CGG - chr1.2:32692765-32692784 None:intergenic 45.0%
ACTGACAGGGTCATTGACAA+TGG + chr1.2:32694830-32694849 MS.gene85770:CDS 45.0%
AGATTCCTCAACCGGATACT+CGG - chr1.2:32692585-32692604 None:intergenic 45.0%
AGGAAGAAACCCCCAATAGT+TGG + chr1.2:32694755-32694774 MS.gene85770:CDS 45.0%
AGTATCCGGTTGAGGAATCT+AGG + chr1.2:32692585-32692604 MS.gene85770:CDS 45.0%
AGTCAGACAGTAGAGAACAC+CGG + chr1.2:32693822-32693841 MS.gene85770:CDS 45.0%
ATAGCTGACAACGATGTGAG+CGG + chr1.2:32692947-32692966 MS.gene85770:CDS 45.0%
ATGTGGGGGTCATAATGAAG+TGG + chr1.2:32692526-32692545 MS.gene85770:CDS 45.0%
ATTCTGTTGGGGTAGTTGGT+GGG + chr1.2:32692558-32692577 MS.gene85770:CDS 45.0%
CACTGTGCATCCATCAAAGA+AGG + chr1.2:32694184-32694203 MS.gene85770:CDS 45.0%
CCTGATCATCCTCATCAGTT+GGG - chr1.2:32694550-32694569 None:intergenic 45.0%
CGACACATATTGGACTGACA+GGG + chr1.2:32694817-32694836 MS.gene85770:CDS 45.0%
CGTGAGGAGATGCAAATAGA+CGG + chr1.2:32693004-32693023 MS.gene85770:CDS 45.0%
GACACTCAAGTTACTGACCA+AGG + chr1.2:32693264-32693283 MS.gene85770:CDS 45.0%
GATGACTCCGAAATGCAAGA+TGG + chr1.2:32694335-32694354 MS.gene85770:CDS 45.0%
GCTATCTTCACTTCAGCTTG+TGG + chr1.2:32694601-32694620 MS.gene85770:CDS 45.0%
GGTTTGTGGTGATTCTGTTG+GGG + chr1.2:32692547-32692566 MS.gene85770:CDS 45.0%
GTATCCGGTTGAGGAATCTA+GGG + chr1.2:32692586-32692605 MS.gene85770:CDS 45.0%
GTCAACAAGGAGTCAAATGG+TGG + chr1.2:32693321-32693340 MS.gene85770:CDS 45.0%
GTCTCAAGACGAGTCGAATA+TGG + chr1.2:32693753-32693772 MS.gene85770:CDS 45.0%
GTGACCCAGTTCCAACTATT+GGG - chr1.2:32694769-32694788 None:intergenic 45.0%
GTGAGAGATGAAGCAGTAGT+TGG + chr1.2:32692683-32692702 MS.gene85770:CDS 45.0%
TAGATGGGTCTGTGGTATGT+GGG + chr1.2:32692510-32692529 MS.gene85770:CDS 45.0%
TCAAAGTCTCCATCTGCCTT+TGG - chr1.2:32694494-32694513 None:intergenic 45.0%
TCACTGCGTCACCTTCTATA+AGG + chr1.2:32694447-32694466 MS.gene85770:CDS 45.0%
TCCTGATCATCCTCATCAGT+TGG - chr1.2:32694551-32694570 None:intergenic 45.0%
TCTTAAAGACCACTCTTGCC+CGG - chr1.2:32695237-32695256 None:intergenic 45.0%
TGACCCAGTTCCAACTATTG+GGG - chr1.2:32694768-32694787 None:intergenic 45.0%
TGCAACACAAGACCAGGAAA+TGG + chr1.2:32695378-32695397 MS.gene85770:CDS 45.0%
TGTTGATGCAACACAAGACC+AGG + chr1.2:32695372-32695391 MS.gene85770:CDS 45.0%
TTCTAGTGACCGATCTCCAA+AGG + chr1.2:32694475-32694494 MS.gene85770:CDS 45.0%
! ACCAGTGATGATGCTTTAGC+AGG + chr1.2:32692857-32692876 MS.gene85770:CDS 45.0%
! ACCTGCTAAAGCATCATCAC+TGG - chr1.2:32692861-32692880 None:intergenic 45.0%
! GCTTTAGCAGGTAGTTCTGT+AGG + chr1.2:32692869-32692888 MS.gene85770:CDS 45.0%
! GTGGTTTGTGGTGATTCTGT+TGG + chr1.2:32692545-32692564 MS.gene85770:CDS 45.0%
! TTTGGAGCTTGTGACTGCTA+AGG + chr1.2:32693971-32693990 MS.gene85770:CDS 45.0%
! TTTGGTGACTTAGCAGAGAC+TGG - chr1.2:32694944-32694963 None:intergenic 45.0%
! TTTTCCTTTCCATCGCGCAA+CGG - chr1.2:32693300-32693319 None:intergenic 45.0%
!! AGTTGATAGAGTAAGCAGCC+GGG + chr1.2:32695216-32695235 MS.gene85770:CDS 45.0%
!! ATTCTCCTGATGGTGATGGT+CGG + chr1.2:32694243-32694262 MS.gene85770:CDS 45.0%
!! GAAGCTTTGAACAGTGCACT+TGG - chr1.2:32695349-32695368 None:intergenic 45.0%
!! TTGGTGGTGAAACCCTAGAT+GGG + chr1.2:32692495-32692514 MS.gene85770:CDS 45.0%
!!! TGCACTGGTTTTTGTTGCAC+TGG - chr1.2:32694884-32694903 None:intergenic 45.0%
!!! TGGGTAACGTTGTTTTGGGA+GGG + chr1.2:32692657-32692676 MS.gene85770:CDS 45.0%
!!! TTGGGTAACGTTGTTTTGGG+AGG + chr1.2:32692656-32692675 MS.gene85770:CDS 45.0%
AAACGATCAAAGCCAGCCGT+AGG - chr1.2:32693954-32693973 None:intergenic 50.0%
AAAGCCAGCCGTAGGAAACT+CGG - chr1.2:32693946-32693965 None:intergenic 50.0%
AAGTGAGAAGTCACCCATGG+TGG + chr1.2:32693517-32693536 MS.gene85770:CDS 50.0%
AATCTCCGCGGTGTGATTCA+CGG - chr1.2:32693062-32693081 None:intergenic 50.0%
ACCCATGGTGTGAAAGTTGG+TGG + chr1.2:32692479-32692498 MS.gene85770:CDS 50.0%
AGATGGGTCTGTGGTATGTG+GGG + chr1.2:32692511-32692530 MS.gene85770:CDS 50.0%
ATCCGTCAGATGCATCTGAG+AGG + chr1.2:32693556-32693575 MS.gene85770:CDS 50.0%
ATTTCGGAGTCATCGGCGTA+GGG - chr1.2:32694329-32694348 None:intergenic 50.0%
CAAGGAGTCAAATGGTGGTC+AGG + chr1.2:32693326-32693345 MS.gene85770:CDS 50.0%
CACATACCACAGACCCATCT+AGG - chr1.2:32692511-32692530 None:intergenic 50.0%
CACCACCAACTTTCACACCA+TGG - chr1.2:32692484-32692503 None:intergenic 50.0%
CCATCACCATCAGGAGAATC+TGG - chr1.2:32694242-32694261 None:intergenic 50.0%
CCCAACTGATGAGGATGATC+AGG + chr1.2:32694547-32694566 MS.gene85770:CDS 50.0%
CTAGATGGGTCTGTGGTATG+TGG + chr1.2:32692509-32692528 MS.gene85770:CDS 50.0%
CTGTAGTTGTGGCTGCTGAT+TGG + chr1.2:32694696-32694715 MS.gene85770:CDS 50.0%
GACCCAGTTCCAACTATTGG+GGG - chr1.2:32694767-32694786 None:intergenic 50.0%
GACGGATCAAATATCTGCCC+AGG - chr1.2:32693543-32693562 None:intergenic 50.0%
GATTCTGTTGGGGTAGTTGG+TGG + chr1.2:32692557-32692576 MS.gene85770:CDS 50.0%
GCAAAGTGAGAAGTCACCCA+TGG + chr1.2:32693514-32693533 MS.gene85770:CDS 50.0%
GCGACACATATTGGACTGAC+AGG + chr1.2:32694816-32694835 MS.gene85770:CDS 50.0%
GGTGACCCAGTTCCAACTAT+TGG - chr1.2:32694770-32694789 None:intergenic 50.0%
GGTGATTCTGTTGGGGTAGT+TGG + chr1.2:32692554-32692573 MS.gene85770:CDS 50.0%
GTCAGACAGTAGAGAACACC+GGG + chr1.2:32693823-32693842 MS.gene85770:CDS 50.0%
GTGACCCATGGTGTGAAAGT+TGG + chr1.2:32692476-32692495 MS.gene85770:CDS 50.0%
TATCTTGTGATCTGCGGCCT+CGG - chr1.2:32692828-32692847 None:intergenic 50.0%
TCAGAAACCACTGTGACCCA+TGG + chr1.2:32692464-32692483 MS.gene85770:CDS 50.0%
TCAGACAGTAGAGAACACCG+GGG + chr1.2:32693824-32693843 MS.gene85770:CDS 50.0%
TCCGTCAGATGCATCTGAGA+GGG + chr1.2:32693557-32693576 MS.gene85770:CDS 50.0%
TGAAACCCTAGATGGGTCTG+TGG + chr1.2:32692502-32692521 MS.gene85770:CDS 50.0%
TGAAACCGTGAATCACACCG+CGG + chr1.2:32693054-32693073 MS.gene85770:CDS 50.0%
TGGTCGGACTCCAGATAATC+TGG + chr1.2:32694259-32694278 MS.gene85770:CDS 50.0%
TGTGTCTCTCCCAACTGATG+AGG + chr1.2:32694538-32694557 MS.gene85770:CDS 50.0%
TTCCGTGATGGTTGTTGCAC+TGG - chr1.2:32694899-32694918 None:intergenic 50.0%
TTCTGTTCGTGCAGGTTCGT+CGG + chr1.2:32693191-32693210 MS.gene85770:intron 50.0%
TTTCACACCATGGGTCACAG+TGG - chr1.2:32692474-32692493 None:intergenic 50.0%
! AGCGCTCTTGCTCCTTTTCT+TGG - chr1.2:32694300-32694319 None:intergenic 50.0%
! CCAGATTCTCCTGATGGTGA+TGG + chr1.2:32694239-32694258 MS.gene85770:CDS 50.0%
! CTCCATCTGCCTTTGGAGAT+CGG - chr1.2:32694487-32694506 None:intergenic 50.0%
! GCTTGTAGTTCTGTTCGTGC+AGG + chr1.2:32693183-32693202 MS.gene85770:intron 50.0%
! GGCTTACGATTAACTTGGGC+TGG - chr1.2:32695010-32695029 None:intergenic 50.0%
! GGCTTTGGTGACTTAACAGC+TGG - chr1.2:32694968-32694987 None:intergenic 50.0%
! GGTGACTTAACAGCTGGCTT+TGG - chr1.2:32694962-32694981 None:intergenic 50.0%
! GTCGGTGTCTATTGAGACTG+AGG + chr1.2:32693209-32693228 MS.gene85770:CDS 50.0%
! TGTGAGCGGTGTTTCTGCTT+TGG + chr1.2:32692961-32692980 MS.gene85770:CDS 50.0%
! TTCTGAAAGCAATCCTGCCG+AGG + chr1.2:32695048-32695067 MS.gene85770:CDS 50.0%
!! ACATTGTGGAGGTGTTGACC+CGG + chr1.2:32694049-32694068 MS.gene85770:CDS 50.0%
!! CGATTAACTTGGGCTGGCTT+TGG - chr1.2:32695004-32695023 None:intergenic 50.0%
!! GTTGGTGGTGAAACCCTAGA+TGG + chr1.2:32692494-32692513 MS.gene85770:CDS 50.0%
!! TAATATGGTGGATGCTGCCG+AGG + chr1.2:32692808-32692827 MS.gene85770:CDS 50.0%
!! TACGGCTGGCTTTGATCGTT+TGG + chr1.2:32693953-32693972 MS.gene85770:CDS 50.0%
!!! GGGTAACGTTGTTTTGGGAG+GGG + chr1.2:32692658-32692677 MS.gene85770:CDS 50.0%
AATATCTGCCCAGGCCACCA+TGG - chr1.2:32693534-32693553 None:intergenic 55.0%
AATGCGGCGGCAGTTGAGTT+GGG + chr1.2:32692638-32692657 MS.gene85770:CDS 55.0%
ACTACCAACTACTGCGGCCT+CGG - chr1.2:32692759-32692778 None:intergenic 55.0%
AGAAGCCGGGAGTGAGAATG+CGG + chr1.2:32692622-32692641 MS.gene85770:CDS 55.0%
ATATCTGCCCAGGCCACCAT+GGG - chr1.2:32693533-32693552 None:intergenic 55.0%
CATTTCGGAGTCATCGGCGT+AGG - chr1.2:32694330-32694349 None:intergenic 55.0%
CTATCCGAGTTTCCTACGGC+TGG + chr1.2:32693939-32693958 MS.gene85770:CDS 55.0%
CTTGTGGCACAAGAACCCCT+TGG + chr1.2:32694617-32694636 MS.gene85770:CDS 55.0%
GACCGATCTCCAAAGGCAGA+TGG + chr1.2:32694482-32694501 MS.gene85770:CDS 55.0%
GATGGGTCTGTGGTATGTGG+GGG + chr1.2:32692512-32692531 MS.gene85770:CDS 55.0%
GCCCTCTCAGATGCATCTGA+CGG - chr1.2:32693561-32693580 None:intergenic 55.0%
GGGACTCCAGATTCTCCTGA+TGG + chr1.2:32694233-32694252 MS.gene85770:CDS 55.0%
! CAATTTGGTGGATGCTGCCG+AGG + chr1.2:32692739-32692758 MS.gene85770:CDS 55.0%
!! AGTTGGTGGGACCGAGTATC+CGG + chr1.2:32692571-32692590 MS.gene85770:CDS 55.0%
!! GCCGCAGTAGTTGGTAGTAC+AGG + chr1.2:32692761-32692780 MS.gene85770:CDS 55.0%
!! GGCTTTGGTGACTTGGTAGC+TGG - chr1.2:32694989-32695008 None:intergenic 55.0%
!! GGTGACTTGGTAGCTGGCTT+TGG - chr1.2:32694983-32695002 None:intergenic 55.0%
!! TGCACTGGTTTCTGTTGCGC+TGG - chr1.2:32694869-32694888 None:intergenic 55.0%
!! TTGGGCTGGCTTTGGTGACT+TGG - chr1.2:32694996-32695015 None:intergenic 55.0%
!!! AGGAGGTTTTTCGGGAGCCT+CGG - chr1.2:32695068-32695087 None:intergenic 55.0%
AGAAGTCACCCATGGTGGCC+TGG + chr1.2:32693522-32693541 MS.gene85770:CDS 60.0%
AGTAAGCAGCCGGGCAAGAG+TGG + chr1.2:32695225-32695244 MS.gene85770:CDS 60.0%
GAAGTCACCCATGGTGGCCT+GGG + chr1.2:32693523-32693542 MS.gene85770:CDS 60.0%
GAATGCGGCGGCAGTTGAGT+TGG + chr1.2:32692637-32692656 MS.gene85770:CDS 60.0%
GAGGCTCCCGAAAAACCTCC+TGG + chr1.2:32695067-32695086 MS.gene85770:CDS 60.0%
GGAGTCCGACCATCACCATC+AGG - chr1.2:32694251-32694270 None:intergenic 60.0%
TGCACCGTTGCGCGATGGAA+AGG + chr1.2:32693293-32693312 MS.gene85770:CDS 60.0%
TGGGACCGAGTATCCGGTTG+AGG + chr1.2:32692577-32692596 MS.gene85770:CDS 60.0%
TTCCATCGCGCAACGGTGCA+TGG - chr1.2:32693293-32693312 None:intergenic 60.0%
AGCCATGCACCGTTGCGCGA+TGG + chr1.2:32693288-32693307 MS.gene85770:CDS 65.0%
AGCCGGGAGTGAGAATGCGG+CGG + chr1.2:32692625-32692644 MS.gene85770:CDS 65.0%
GCAACGGTGCATGGCTCCCT+TGG - chr1.2:32693284-32693303 None:intergenic 65.0%
TGCCGCCGCATTCTCACTCC+CGG - chr1.2:32692630-32692649 None:intergenic 65.0%
!!! GGTTTTTCGGGAGCCTCGGC+AGG - chr1.2:32695064-32695083 None:intergenic 65.0%
GCTGCCGAGGCCGCAGTAGT+TGG + chr1.2:32692752-32692771 MS.gene85770:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 32692443 32695438 32692443 ID=MS.gene85770
chr1.2 mRNA 32692443 32695438 32692443 ID=MS.gene85770.t1;Parent=MS.gene85770
chr1.2 exon 32692443 32693085 32692443 ID=MS.gene85770.t1.exon1;Parent=MS.gene85770.t1
chr1.2 CDS 32692443 32693085 32692443 ID=cds.MS.gene85770.t1;Parent=MS.gene85770.t1
chr1.2 exon 32693205 32695438 32693205 ID=MS.gene85770.t1.exon2;Parent=MS.gene85770.t1
chr1.2 CDS 32693205 32695438 32693205 ID=cds.MS.gene85770.t1;Parent=MS.gene85770.t1
Gene Sequence

>MS.gene85770

ATGGAAGAACCAGAAAATGTTTCAGAAACCACTGTGACCCATGGTGTGAAAGTTGGTGGTGAAACCCTAGATGGGTCTGTGGTATGTGGGGGTCATAATGAAGTGGTTTGTGGTGATTCTGTTGGGGTAGTTGGTGGGACCGAGTATCCGGTTGAGGAATCTAGGGTTGTAGAATTTGAAGAAGCCGGGAGTGAGAATGCGGCGGCAGTTGAGTTGGGTAACGTTGTTTTGGGAGGGGAAGTGAGAGATGAAGCAGTAGTTGGTAGTACAGTTATCGAAACAACAATCGGCGACTTCAATTTGGTGGATGCTGCCGAGGCCGCAGTAGTTGGTAGTACAGGTGTCGAAACAACAATCGGTGACTTTAATATGGTGGATGCTGCCGAGGCCGCAGATCACAAGATAATAAATACTACCAGTGATGATGCTTTAGCAGGTAGTTCTGTAGGGGAAGAAAATGTACAGTGCTTTAGCCTTGAAAAGTGCAACTCAAAGGAGCAATTGATAGCTGACAACGATGTGAGCGGTGTTTCTGCTTTGGATGCTCAACAATCTGATGTACGTGAGGAGATGCAAATAGACGGTGAAGATCAACAAGGGACAGAAAGAAATGAAACCGTGAATCACACCGCGGAGATTAAAGGTAAGAAGATATCTGCCGATTTCATTGGTGTTTTTTCCAGCTTAACTAGTGGTTACTTGTAGTAAATGAAAATGTTGTTTAGTTTCTAGATAATGATGCTTGTAGTTCTGTTCGTGCAGGTTCGTCGGTGTCTATTGAGACTGAGGAAAACTTGGATGCTAATGCAATTGTAGAAGAAGACACTCAAGTTACTGACCAAGGGAGCCATGCACCGTTGCGCGATGGAAAGGAAAAAGTCAACAAGGAGTCAAATGGTGGTCAGGAAATTGAAAAAGTTAATGAAGTTGGGAAGAGAAAACTAAGAGATAGAAAAGCCGGAAAACATGCACTTGTGAAGCCTGGTAGTTCAGAAATAGTTCATCAGGCACGATATTTGCTGCCAAAAGAAAAAGAAGAAGGCGAGTTTTCTCTCTCAGATATGGTTTGGGGCAAAGTGAGAAGTCACCCATGGTGGCCTGGGCAGATATTTGATCCGTCAGATGCATCTGAGAGGGCAAAGAAGCATTATAAAAAAGATTCCTATTTCGTTGCATATTTTGGGGACAAAACATTTGCATGGAATGAAGCATCTAAGCTAAAGCATTTTAGGGCACATTTCTCCACAATTGAAGAGCATAGGAATCGTTCAGAATCATTCCAGAGTGCTATTAATTGTGCTTTGGATGAAGTCTCAAGACGAGTCGAATATGGGCTAGCATGTTCTTGTATACCTAAAGACACCTATGACATGATTAAAAGTCAGACAGTAGAGAACACCGGGGTTCAACAAGAAATAAGTATCACACATGGGGTGGATGAGTCTCTAAATGCTGACTCATTTTCACCTTTGAATCTTATAGAATATGTGAAAACACTATCCGAGTTTCCTACGGCTGGCTTTGATCGTTTGGAGCTTGTGACTGCTAAGGCTCAGCTGCTTGCTTTTAATCGTTTTAAGGGTTTCTCTTGCTTGCCAGAAATACAACATTGTGGAGGTGTTGACCCGGACCATTCATTTTTTGTTGACGATGAACAAGATTTGTGTGAAGATACTGAGAATGCAACTCCTGTAGTTAAAAAAGGCAGCACCCGTCAAAAGCGTAAATATAAGTTCAAGGACACTGTGCATCCATCAAAGAAGGAAAAAAGGATGTCAGACCTAATGAATGGGACTCCAGATTCTCCTGATGGTGATGGTCGGACTCCAGATAATCTGGTTTCACCTGAACATTCCAAGAAAAGGAGCAAGAGCGCTATTGATCCCTACGCCGATGACTCCGAAATGCAAGATGGGAGAAAAACAATTTCTGTTGCAAAAGTTTCAAATACCACAAAGTCATCGTTTAAAATCGGTGATTGTATTCGTCGGGCTGCTAGTCAACTCACTGCGTCACCTTCTATAAGGAAGTGTTCTAGTGACCGATCTCCAAAGGCAGATGGAGACTTTGATGTTTTTCCTGATAATGAATCAGATGTGTCTCTCCCAACTGATGAGGATGATCAGGATTCGACAGAATATTCGTCTCTAGATGATTTGCTATCTTCACTTCAGCTTGTGGCACAAGAACCCCTTGGAGAGTATACTTTTCTGAATGGCAGCATAGTCAGCTTCTTCTCTGATTTCAGAAATTCTGTAGTTGTGGCTGCTGATTGGAAAGAGATTTTACGTACGGATACATTTGGTATTAAGAGGAAGAAACCCCCAATAGTTGGAACTGGGTCACCTGAAACATTTGAATTTGAAGATATGAGCGACACATATTGGACTGACAGGGTCATTGACAATGGCACTGAAGAGAAACCAGCGCAACAGAAACCAGTGCAACAAAAACCAGTGCAACAACCATCACGGAAAAACCAGAAAAAAGACGAACAACCAGTCTCTGCTAAGTCACCAAAGCCAGCTGTTAAGTCACCAAAGCCAGCTACCAAGTCACCAAAGCCAGCCCAAGTTAATCGTAAGCCCTATACCAAGAAAAACAATTCTGAAAGCAATCCTGCCGAGGCTCCCGAAAAACCTCCTGGTTATATAGATGACAATGCTCCTGCTGAACTTGTTATGAACTTTGCCGAGCTCAATTCTGTTCCCTTAGAAGCAAACCTTAACAAAATGTTTAAGCGTTTTGGACCACTTAAGGAATCTGAAACAGAAGTTGATAGAGTAAGCAGCCGGGCAAGAGTGGTCTTTAAGAAATGTGTAGATGCAGAGGTAGCTTTCAATAGTGCTAAGAAGTTCAACATATTTGGATCAGTACTCGTAAATTACCAGCTTAATTACACTCCAAGTGCACTGTTCAAAGCTTCATCTGTTGATGCAACACAAGACCAGGAAATGGTTTTCGATCTCTCCAATTTTGAAGTTAATATGGTCTAG
Protein sequence

>MS.gene85770.t1

MEEPENVSETTVTHGVKVGGETLDGSVVCGGHNEVVCGDSVGVVGGTEYPVEESRVVEFEEAGSENAAAVELGNVVLGGEVRDEAVVGSTVIETTIGDFNLVDAAEAAVVGSTGVETTIGDFNMVDAAEAADHKIINTTSDDALAGSSVGEENVQCFSLEKCNSKEQLIADNDVSGVSALDAQQSDVREEMQIDGEDQQGTERNETVNHTAEIKGSSVSIETEENLDANAIVEEDTQVTDQGSHAPLRDGKEKVNKESNGGQEIEKVNEVGKRKLRDRKAGKHALVKPGSSEIVHQARYLLPKEKEEGEFSLSDMVWGKVRSHPWWPGQIFDPSDASERAKKHYKKDSYFVAYFGDKTFAWNEASKLKHFRAHFSTIEEHRNRSESFQSAINCALDEVSRRVEYGLACSCIPKDTYDMIKSQTVENTGVQQEISITHGVDESLNADSFSPLNLIEYVKTLSEFPTAGFDRLELVTAKAQLLAFNRFKGFSCLPEIQHCGGVDPDHSFFVDDEQDLCEDTENATPVVKKGSTRQKRKYKFKDTVHPSKKEKRMSDLMNGTPDSPDGDGRTPDNLVSPEHSKKRSKSAIDPYADDSEMQDGRKTISVAKVSNTTKSSFKIGDCIRRAASQLTASPSIRKCSSDRSPKADGDFDVFPDNESDVSLPTDEDDQDSTEYSSLDDLLSSLQLVAQEPLGEYTFLNGSIVSFFSDFRNSVVVAADWKEILRTDTFGIKRKKPPIVGTGSPETFEFEDMSDTYWTDRVIDNGTEEKPAQQKPVQQKPVQQPSRKNQKKDEQPVSAKSPKPAVKSPKPATKSPKPAQVNRKPYTKKNNSESNPAEAPEKPPGYIDDNAPAELVMNFAELNSVPLEANLNKMFKRFGPLKESETEVDRVSSRARVVFKKCVDAEVAFNSAKKFNIFGSVLVNYQLNYTPSALFKASSVDATQDQEMVFDLSNFEVNMV