Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86430.t1 | XP_003597930.2 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.40E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86430.t1 | P42322 | 37.8 | 172 | 100 | 2 | 1 | 167 | 1 | 170 | 1.6e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86430.t1 | B7FH75 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 2.5e-93 | 350.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene86430 | MS.gene72809 | PPI |
| MS.gene86430 | MS.gene82121 | PPI |
| MS.gene86430 | MS.gene028111 | PPI |
| MS.gene86430 | MS.gene37035 | PPI |
| MS.gene86430 | MS.gene83474 | PPI |
| MS.gene86430 | MS.gene022826 | PPI |
| MS.gene86430 | MS.gene020092 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86430.t1 | MTR_2g104130 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 5.87e-128 | 356 |
| MS.gene86430.t1 | MTR_4g130410 | 70.286 | 175 | 52 | 0 | 1 | 175 | 1 | 175 | 1.37e-91 | 264 |
| MS.gene86430.t1 | MTR_4g130410 | 71.429 | 161 | 46 | 0 | 1 | 161 | 1 | 161 | 5.36e-84 | 245 |
| MS.gene86430.t1 | MTR_1g076650 | 27.517 | 149 | 94 | 5 | 22 | 161 | 3 | 146 | 1.56e-11 | 59.7 |
| MS.gene86430.t1 | MTR_1g071150 | 27.517 | 149 | 95 | 5 | 21 | 161 | 2 | 145 | 2.35e-11 | 58.9 |
| MS.gene86430.t1 | MTR_1g041285 | 26.797 | 153 | 98 | 5 | 22 | 165 | 3 | 150 | 2.44e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86430.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86430.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86430.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86430.t1 | AT3G22930 | 27.152 | 151 | 96 | 5 | 24 | 165 | 28 | 173 | 5.16e-12 | 61.6 |
Find 23 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAGGTTCTTGGCCATTC+TGG | 0.182957 | 2.1:-1690560 | MS.gene86430:intron |
| TGTACTAAATGCAGATAAAA+AGG | 0.239607 | 2.1:+1691571 | None:intergenic |
| GACATTGGATGATTTCATTA+AGG | 0.296420 | 2.1:-1690759 | MS.gene86430:intron |
| TTAAATTTGTCACAGGTTCT+TGG | 0.342309 | 2.1:-1690569 | MS.gene86430:intron |
| TTGATTTCTCAGGAAGTCTT+AGG | 0.365914 | 2.1:-1690824 | MS.gene86430:intron |
| AGGTTACTCAAGATGGTTGA+TGG | 0.393812 | 2.1:-1691617 | MS.gene86430:CDS |
| TGTTATGAATCCACTTTCTC+AGG | 0.398536 | 2.1:-1691986 | MS.gene86430:intron |
| GGTGTCTTTCAAAGACATAT+TGG | 0.407546 | 2.1:-1691236 | MS.gene86430:CDS |
| ATTTGATTCAGTTTCTCAAC+AGG | 0.411656 | 2.1:-1692100 | MS.gene86430:intron |
| GAAGTTCTAAAAGATTTGTC+TGG | 0.444669 | 2.1:-1691214 | MS.gene86430:CDS |
| ATCCTGCTTCTTTCAGAACC+TGG | 0.474604 | 2.1:+1690801 | None:intergenic |
| CAACTTGACAGAAATGCTAA+AGG | 0.478282 | 2.1:-1692048 | MS.gene86430:CDS |
| TTTGCAGAGGTTACTCAAGA+TGG | 0.478552 | 2.1:-1691624 | MS.gene86430:intron |
| TGATATGATACCTGAGAAAG+TGG | 0.501757 | 2.1:+1691976 | None:intergenic |
| TCTGCATTTAGTACAAAAGC+TGG | 0.505330 | 2.1:-1691563 | MS.gene86430:CDS |
| GCTAACTCAATATGATATTG+AGG | 0.520808 | 2.1:-1692231 | MS.gene86430:CDS |
| TTCTCAGGAAGTCTTAGGCC+AGG | 0.525529 | 2.1:-1690819 | MS.gene86430:intron |
| GGCCAGGTTCTGAAAGAAGC+AGG | 0.530644 | 2.1:-1690803 | MS.gene86430:CDS |
| GTATATGATTCAGATCGTAA+TGG | 0.556045 | 2.1:-1691262 | MS.gene86430:CDS |
| AAAAGACTGCTACTTGACAT+TGG | 0.563411 | 2.1:-1690774 | MS.gene86430:CDS |
| AGTGGATTCATAACAAACTC+AGG | 0.575670 | 2.1:+1691994 | None:intergenic |
| TGATTCAGATCGTAATGGAA+AGG | 0.613377 | 2.1:-1691257 | MS.gene86430:CDS |
| CATGTCTGATGATCAAAGAG+AGG | 0.654475 | 2.1:-1691185 | MS.gene86430:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTATCTAAATTATTT+AGG | + | chr2.1:1691347-1691366 | None:intergenic | 10.0% |
| !! | TTTATATTTAAATTTGTCAC+AGG | - | chr2.1:1692201-1692220 | MS.gene86430:intron | 15.0% |
| !!! | ATTTTCTTATATATCTCTTT+TGG | - | chr2.1:1692048-1692067 | MS.gene86430:CDS | 15.0% |
| !!! | TTTTATGAAAATGGTATATT+TGG | + | chr2.1:1690983-1691002 | None:intergenic | 15.0% |
| !!! | TTTTATTCTTTATTAGTGTT+TGG | - | chr2.1:1691295-1691314 | MS.gene86430:intron | 15.0% |
| !!! | TTTTATTGGCTTTTATTTAA+TGG | + | chr2.1:1691852-1691871 | None:intergenic | 15.0% |
| !! | ACACATTTATTTAATTGAGT+CGG | + | chr2.1:1690915-1690934 | None:intergenic | 20.0% |
| !! | ACTCAATTAAATAAATGTGT+TGG | - | chr2.1:1690915-1690934 | MS.gene86430:intron | 20.0% |
| !! | ATCATAAATTAGAAGATTTC+AGG | - | chr2.1:1691361-1691380 | MS.gene86430:intron | 20.0% |
| !! | GAAATGAAATATTCGTATAT+AGG | + | chr2.1:1691830-1691849 | None:intergenic | 20.0% |
| !!! | ATGTGTTTACTATTTTATAC+AGG | - | chr2.1:1691085-1691104 | MS.gene86430:intron | 20.0% |
| !!! | GTTCTTTCTAATTGTAATTT+TGG | - | chr2.1:1692156-1692175 | MS.gene86430:intron | 20.0% |
| !!! | TGTGGAAAAAAGTTTTTTAT+TGG | + | chr2.1:1691866-1691885 | None:intergenic | 20.0% |
| !!! | TTTGAATTTTAAAGACTTTG+TGG | - | chr2.1:1691183-1691202 | MS.gene86430:intron | 20.0% |
| !!! | TTTTTTTGAAGAAACTCTTT+TGG | - | chr2.1:1692086-1692105 | MS.gene86430:CDS | 20.0% |
| ! | ACATTTATTTAATTGAGTCG+GGG | + | chr2.1:1690913-1690932 | None:intergenic | 25.0% |
| ! | ACTATAAGTACCCTAATTAA+AGG | + | chr2.1:1691462-1691481 | None:intergenic | 25.0% |
| ! | AGAAGAAGAAGAAAAAGATA+AGG | + | chr2.1:1690617-1690636 | None:intergenic | 25.0% |
| ! | AGTATATCAAACTCAACATA+GGG | + | chr2.1:1691737-1691756 | None:intergenic | 25.0% |
| ! | ATTCTTTATTAGTGTTTGGA+TGG | - | chr2.1:1691299-1691318 | MS.gene86430:intron | 25.0% |
| ! | CACATTTATTTAATTGAGTC+GGG | + | chr2.1:1690914-1690933 | None:intergenic | 25.0% |
| ! | TAGTATATCAAACTCAACAT+AGG | + | chr2.1:1691738-1691757 | None:intergenic | 25.0% |
| ! | TCAACTAAGTCCTTTAATTA+GGG | - | chr2.1:1691449-1691468 | MS.gene86430:intron | 25.0% |
| ! | TGTACTAAATGCAGATAAAA+AGG | + | chr2.1:1691209-1691228 | None:intergenic | 25.0% |
| ! | TTCAACTAAGTCCTTTAATT+AGG | - | chr2.1:1691448-1691467 | MS.gene86430:intron | 25.0% |
| !! | AAGAATAAAATCTGAAGCAA+TGG | + | chr2.1:1691285-1691304 | None:intergenic | 25.0% |
| !! | TACAGAAGCATATAGTTATT+TGG | + | chr2.1:1691623-1691642 | None:intergenic | 25.0% |
| AACATTAAGCAATCAAAGCA+AGG | + | chr2.1:1691026-1691045 | None:intergenic | 30.0% | |
| AATCATTTATGTATGTCCTG+TGG | - | chr2.1:1690586-1690605 | MS.gene86430:intron | 30.0% | |
| AGCAAAGTAAGTTTCCATTA+AGG | - | chr2.1:1691685-1691704 | MS.gene86430:intron | 30.0% | |
| ATTTGATTCAGTTTCTCAAC+AGG | - | chr2.1:1690677-1690696 | MS.gene86430:intron | 30.0% | |
| GAAGAAGAAAAAGATAAGGA+AGG | + | chr2.1:1690613-1690632 | None:intergenic | 30.0% | |
| GACATTGGATGATTTCATTA+AGG | - | chr2.1:1692018-1692037 | MS.gene86430:CDS | 30.0% | |
| GCTAACTCAATATGATATTG+AGG | - | chr2.1:1690546-1690565 | MS.gene86430:CDS | 30.0% | |
| GTATATGATTCAGATCGTAA+TGG | - | chr2.1:1691515-1691534 | MS.gene86430:intron | 30.0% | |
| TAACAATTGATGCACCTTAA+TGG | + | chr2.1:1691702-1691721 | None:intergenic | 30.0% | |
| TTAAATTTGTCACAGGTTCT+TGG | - | chr2.1:1692208-1692227 | MS.gene86430:CDS | 30.0% | |
| ! | AAAACGTACGTTCAACTTTT+TGG | + | chr2.1:1691246-1691265 | None:intergenic | 30.0% |
| ! | AAATGGTATATTTGGAACAG+TGG | + | chr2.1:1690975-1690994 | None:intergenic | 30.0% |
| ! | TTACTGTTCTTTGATTTCTC+AGG | - | chr2.1:1691943-1691962 | MS.gene86430:intron | 30.0% |
| !! | GAAGTTCTAAAAGATTTGTC+TGG | - | chr2.1:1691563-1691582 | MS.gene86430:CDS | 30.0% |
| AGAAGATGTCCACTATAAGT+AGG | - | chr2.1:1691049-1691068 | MS.gene86430:intron | 35.0% | |
| CAACTTGACAGAAATGCTAA+AGG | - | chr2.1:1690729-1690748 | MS.gene86430:intron | 35.0% | |
| GAAGATGTCCACTATAAGTA+GGG | - | chr2.1:1691050-1691069 | MS.gene86430:intron | 35.0% | |
| GGTGTCTTTCAAAGACATAT+TGG | - | chr2.1:1691541-1691560 | MS.gene86430:intron | 35.0% | |
| GTTTGTGTACTTATTTGCAG+AGG | - | chr2.1:1691140-1691159 | MS.gene86430:intron | 35.0% | |
| TCTGCATTTAGTACAAAAGC+TGG | - | chr2.1:1691214-1691233 | MS.gene86430:CDS | 35.0% | |
| TGATATGATACCTGAGAAAG+TGG | + | chr2.1:1690804-1690823 | None:intergenic | 35.0% | |
| TGTTATGAATCCACTTTCTC+AGG | - | chr2.1:1690791-1690810 | MS.gene86430:CDS | 35.0% | |
| TTGATTTCTCAGGAAGTCTT+AGG | - | chr2.1:1691953-1691972 | MS.gene86430:intron | 35.0% | |
| ! | AAAAGACTGCTACTTGACAT+TGG | - | chr2.1:1692003-1692022 | MS.gene86430:CDS | 35.0% |
| ! | AGTGGATTCATAACAAACTC+AGG | + | chr2.1:1690786-1690805 | None:intergenic | 35.0% |
| ! | TGATTCAGATCGTAATGGAA+AGG | - | chr2.1:1691520-1691539 | MS.gene86430:intron | 35.0% |
| !! | CATCCATTTTTACTCCAGAA+TGG | + | chr2.1:1692234-1692253 | None:intergenic | 35.0% |
| AAAAGATAAGGAAGGACCAC+AGG | + | chr2.1:1690605-1690624 | None:intergenic | 40.0% | |
| AGGTTACTCAAGATGGTTGA+TGG | - | chr2.1:1691160-1691179 | MS.gene86430:intron | 40.0% | |
| CATGTCTGATGATCAAAGAG+AGG | - | chr2.1:1691592-1691611 | MS.gene86430:CDS | 40.0% | |
| CCAATACAGCACAACTGAAA+AGG | + | chr2.1:1691893-1691912 | None:intergenic | 40.0% | |
| TATCCTAGCCCTACTTATAG+TGG | + | chr2.1:1691061-1691080 | None:intergenic | 40.0% | |
| TTTGCAGAGGTTACTCAAGA+TGG | - | chr2.1:1691153-1691172 | MS.gene86430:intron | 40.0% | |
| ! | CCTTTTCAGTTGTGCTGTAT+TGG | - | chr2.1:1691890-1691909 | MS.gene86430:intron | 40.0% |
| !! | TGGCCATTCTGGAGTAAAAA+TGG | - | chr2.1:1692228-1692247 | MS.gene86430:CDS | 40.0% |
| ATCCTGCTTCTTTCAGAACC+TGG | + | chr2.1:1691979-1691998 | None:intergenic | 45.0% | |
| TGTCCACTATAAGTAGGGCT+AGG | - | chr2.1:1691055-1691074 | MS.gene86430:intron | 45.0% | |
| ! | CACAACTGAAAAGGTGTGTG+TGG | + | chr2.1:1691884-1691903 | None:intergenic | 45.0% |
| !!! | TTTATTTTATTTTATGAAAA+TGG | + | chr2.1:1690992-1691011 | None:intergenic | 5.0% |
| TCACAGGTTCTTGGCCATTC+TGG | - | chr2.1:1692217-1692236 | MS.gene86430:CDS | 50.0% | |
| TTCTCAGGAAGTCTTAGGCC+AGG | - | chr2.1:1691958-1691977 | MS.gene86430:intron | 50.0% | |
| !! | GGCCAGGTTCTGAAAGAAGC+AGG | - | chr2.1:1691974-1691993 | MS.gene86430:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 1690526 | 1692273 | 1690526 | ID=MS.gene86430 |
| chr2.1 | mRNA | 1690526 | 1692273 | 1690526 | ID=MS.gene86430.t1;Parent=MS.gene86430 |
| chr2.1 | exon | 1692204 | 1692273 | 1692204 | ID=MS.gene86430.t1.exon1;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1692204 | 1692273 | 1692204 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| chr2.1 | exon | 1691987 | 1692111 | 1691987 | ID=MS.gene86430.t1.exon2;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1691987 | 1692111 | 1691987 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| chr2.1 | exon | 1691543 | 1691639 | 1691543 | ID=MS.gene86430.t1.exon3;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1691543 | 1691639 | 1691543 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| chr2.1 | exon | 1691186 | 1691295 | 1691186 | ID=MS.gene86430.t1.exon4;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1691186 | 1691295 | 1691186 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| chr2.1 | exon | 1690760 | 1690834 | 1690760 | ID=MS.gene86430.t1.exon5;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1690760 | 1690834 | 1690760 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| chr2.1 | exon | 1690526 | 1690576 | 1690526 | ID=MS.gene86430.t1.exon6;Parent=MS.gene86430.t1 |
| chr2.1 | CDS | 1690526 | 1690576 | 1690526 | ID=cds.MS.gene86430.t1;Parent=MS.gene86430.t1 |
| Gene Sequence |
| Protein sequence |
