AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86828.t1 MTR_1g077990 96.774 403 10 1 1 400 1 403 0.0 793
MS.gene86828.t1 MTR_2g086530 58.772 342 136 3 10 348 6 345 2.73e-145 418
MS.gene86828.t1 MTR_2g086530 61.950 318 118 2 32 348 9 324 3.42e-144 415
MS.gene86828.t1 MTR_4g052970 59.190 321 130 1 30 349 28 348 9.57e-142 409
MS.gene86828.t1 MTR_4g084480 54.039 359 160 3 5 359 3 360 7.76e-141 407
MS.gene86828.t1 MTR_4g084480 54.039 359 160 3 5 359 3 360 1.87e-140 406
MS.gene86828.t1 MTR_4g084480 55.014 349 152 3 15 359 6 353 6.68e-140 404
MS.gene86828.t1 MTR_2g019345 51.026 341 156 3 17 347 1 340 1.96e-122 360
MS.gene86828.t1 MTR_4g010200 49.580 357 172 3 11 359 7 363 3.16e-119 352
MS.gene86828.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 1.38e-110 329
MS.gene86828.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 2.29e-110 329
MS.gene86828.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 2.48e-110 329
MS.gene86828.t1 MTR_5g085580 49.408 338 167 4 26 360 20 356 1.96e-103 314
MS.gene86828.t1 MTR_1g056640 49.091 330 164 4 30 356 37 365 4.28e-103 314
MS.gene86828.t1 MTR_3g116510 45.326 353 189 3 13 361 8 360 3.81e-102 311
MS.gene86828.t1 MTR_1g022160 46.894 322 168 3 31 349 24 345 1.34e-101 308
MS.gene86828.t1 MTR_1g022160 46.894 322 168 3 31 349 24 345 8.70e-101 308
MS.gene86828.t1 MTR_1g022160 46.894 322 168 3 31 349 24 345 9.23e-101 308
MS.gene86828.t1 MTR_1g022160 46.894 322 168 3 31 349 24 345 1.07e-100 308
MS.gene86828.t1 MTR_1g007810 46.707 334 169 5 16 345 1 329 2.23e-100 303
MS.gene86828.t1 MTR_3g080410 46.667 345 180 4 24 365 19 362 1.27e-99 301
MS.gene86828.t1 MTR_4g069940 43.343 353 196 3 13 361 9 361 8.85e-99 302
MS.gene86828.t1 MTR_6g088885 42.149 363 202 5 10 365 5 366 7.98e-95 293
MS.gene86828.t1 MTR_7g026340 44.615 325 171 7 26 347 18 336 4.59e-92 285
MS.gene86828.t1 MTR_8g012400 41.691 343 193 5 13 349 11 352 2.44e-85 268
MS.gene86828.t1 MTR_4g083500 45.566 327 172 6 24 347 37 360 9.46e-83 263
MS.gene86828.t1 MTR_7g053500 39.655 348 201 6 32 371 27 373 4.01e-81 257
MS.gene86828.t1 MTR_4g088155 38.953 344 201 5 14 349 6 348 7.43e-81 256
MS.gene86828.t1 MTR_8g102340 44.099 322 141 4 31 349 28 313 8.27e-81 256
MS.gene86828.t1 MTR_8g085720 39.039 333 197 4 31 358 26 357 4.78e-76 244
MS.gene86828.t1 MTR_3g083580 36.022 372 224 7 16 374 7 377 8.05e-75 241
MS.gene86828.t1 MTR_4g076440 38.393 336 194 8 15 349 6 329 1.74e-73 233
MS.gene86828.t1 MTR_4g132280 40.432 324 187 4 31 349 45 367 4.62e-73 238
MS.gene86828.t1 MTR_1g031900 40.741 324 180 5 31 346 24 343 7.73e-73 236
MS.gene86828.t1 MTR_1g031900 40.367 327 183 5 31 349 24 346 1.76e-72 236
MS.gene86828.t1 MTR_4g076490 40.122 329 186 7 21 349 87 404 1.51e-71 231
MS.gene86828.t1 MTR_3g065460 35.121 373 230 6 12 378 4 370 3.02e-71 232
MS.gene86828.t1 MTR_5g078200 36.264 364 219 6 16 367 6 368 6.93e-71 234
MS.gene86828.t1 MTR_4g076255 39.506 324 184 6 26 349 20 331 1.54e-70 225
MS.gene86828.t1 MTR_5g078200 37.500 336 199 5 42 367 1 335 7.86e-68 226
MS.gene86828.t1 MTR_1g052430 39.640 333 192 6 27 357 28 353 2.06e-67 219
MS.gene86828.t1 MTR_4g076430 37.500 336 197 9 15 349 6 329 3.05e-67 216
MS.gene86828.t1 MTR_7g081350 37.463 339 195 9 16 346 6 335 1.22e-66 215
MS.gene86828.t1 MTR_4g076500 38.650 326 188 8 27 349 74 390 3.60e-66 216
MS.gene86828.t1 MTR_4g076470 37.798 336 196 9 15 349 6 329 6.19e-66 213
MS.gene86828.t1 MTR_3g116510 46.903 226 119 1 137 361 1 226 2.13e-65 212
MS.gene86828.t1 MTR_2g034480 37.278 338 202 8 14 348 9 339 2.41e-65 213
MS.gene86828.t1 MTR_7g053500 40.214 281 160 5 98 371 17 296 2.98e-63 208
MS.gene86828.t1 MTR_4g076570 37.690 329 198 5 28 355 19 341 1.10e-62 206
MS.gene86828.t1 MTR_8g012400 42.023 257 143 4 98 349 17 272 6.64e-62 204
MS.gene86828.t1 MTR_7g081370 34.627 335 197 6 13 346 10 323 1.28e-61 202
MS.gene86828.t1 MTR_2g034470 37.386 329 195 8 24 349 23 343 2.04e-61 202
MS.gene86828.t1 MTR_2g034440 37.304 319 189 8 24 339 15 325 5.50e-60 198
MS.gene86828.t1 MTR_2g034470 37.143 315 187 8 38 349 2 308 1.18e-57 192
MS.gene86828.t1 MTR_2g027560 35.072 345 207 12 24 358 22 359 1.01e-54 188
MS.gene86828.t1 MTR_1g010120 34.493 345 205 13 13 346 9 343 2.73e-53 184
MS.gene86828.t1 MTR_8g092070 35.671 328 195 11 31 349 34 354 4.20e-53 186
MS.gene86828.t1 MTR_7g085390 33.429 350 211 12 13 349 7 347 8.15e-53 183
MS.gene86828.t1 MTR_6g032820 32.849 344 208 11 15 344 10 344 3.47e-52 181
MS.gene86828.t1 MTR_3g108600 34.670 349 191 13 8 346 8 329 2.71e-50 176
MS.gene86828.t1 MTR_4g112025 33.807 352 211 13 8 346 8 350 3.29e-49 174
MS.gene86828.t1 MTR_4g124440 34.463 354 209 15 7 347 5 348 4.05e-48 171
MS.gene86828.t1 MTR_3g095050 34.251 327 199 12 31 349 34 352 9.04e-47 167
MS.gene86828.t1 MTR_1g053320 33.724 341 205 13 19 346 18 350 2.67e-46 166
MS.gene86828.t1 MTR_5g081720 35.276 326 191 12 31 345 33 349 2.29e-42 153
MS.gene86828.t1 MTR_3g031770 29.738 343 225 10 24 358 20 354 6.81e-33 129
MS.gene86828.t1 MTR_4g134280 31.579 247 156 9 118 358 3 242 2.66e-29 117
MS.gene86828.t1 MTR_4g036775 41.129 124 69 2 247 370 80 199 3.08e-17 80.5
MS.gene86828.t1 MTR_4g036680 42.400 125 66 4 247 370 80 199 6.08e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86828.t1 AT1G32860 61.266 395 143 3 8 393 7 400 1.21e-167 476
MS.gene86828.t1 AT5G42100 67.391 322 101 2 30 347 26 347 2.36e-160 458
MS.gene86828.t1 AT5G42100 67.391 322 101 2 30 347 26 347 4.23e-160 457
MS.gene86828.t1 AT2G27500 61.846 325 122 2 27 349 25 349 1.05e-149 429
MS.gene86828.t1 AT2G27500 61.846 325 122 2 27 349 25 349 2.42e-149 429
MS.gene86828.t1 AT4G18340 49.856 347 173 1 13 358 16 362 5.10e-131 382
MS.gene86828.t1 AT4G18340 49.856 347 173 1 13 358 16 362 6.91e-131 381
MS.gene86828.t1 AT1G30080 54.769 325 146 1 24 347 29 353 1.32e-129 378
MS.gene86828.t1 AT1G30080 54.769 325 146 1 24 347 29 353 2.45e-129 379
MS.gene86828.t1 AT2G27500 62.731 271 99 2 81 349 1 271 2.46e-123 360
MS.gene86828.t1 AT3G15800 54.242 330 147 4 32 357 44 373 2.09e-121 358
MS.gene86828.t1 AT2G26600 51.368 329 158 2 32 358 35 363 1.19e-114 340
MS.gene86828.t1 AT4G26830 44.724 398 191 8 13 407 8 379 2.74e-107 324
MS.gene86828.t1 AT4G26830 48.000 350 169 5 25 371 23 362 2.13e-106 322
MS.gene86828.t1 AT2G05790 46.719 381 192 6 30 407 24 396 3.58e-106 322
MS.gene86828.t1 AT5G42720 46.307 352 183 4 16 361 8 359 1.18e-104 317
MS.gene86828.t1 AT4G34480 49.541 327 163 2 31 355 26 352 3.62e-104 312
MS.gene86828.t1 AT5G24318 47.256 328 165 5 24 347 22 345 6.40e-104 312
MS.gene86828.t1 AT5G24318 45.845 349 179 6 13 357 13 355 1.92e-102 311
MS.gene86828.t1 AT5G24318 45.845 349 179 6 13 357 13 355 2.41e-102 311
MS.gene86828.t1 AT2G16230 46.988 332 174 2 31 360 25 356 2.05e-101 310
MS.gene86828.t1 AT4G34480 48.910 321 162 2 31 349 26 346 7.71e-100 306
MS.gene86828.t1 AT5G55180 46.933 326 169 4 31 353 27 351 4.99e-99 303
MS.gene86828.t1 AT5G55180 46.933 326 169 4 31 353 27 351 5.77e-99 303
MS.gene86828.t1 AT2G26600 52.091 263 125 1 97 358 7 269 3.83e-92 280
MS.gene86828.t1 AT5G56590 45.031 322 174 3 31 349 26 347 3.55e-91 284
MS.gene86828.t1 AT3G07320 43.353 346 189 5 32 371 26 370 7.75e-90 279
MS.gene86828.t1 AT4G29360 45.428 339 179 4 13 349 12 346 8.00e-88 276
MS.gene86828.t1 AT4G29360 46.201 329 171 4 23 349 32 356 1.47e-86 273
MS.gene86828.t1 AT4G14080 41.499 347 189 6 8 349 27 364 3.22e-85 268
MS.gene86828.t1 AT3G23770 41.463 369 199 8 21 376 30 394 8.02e-84 264
MS.gene86828.t1 AT2G01630 42.284 324 181 4 31 349 23 345 5.12e-83 259
MS.gene86828.t1 AT2G01630 42.284 324 181 4 31 349 23 345 1.67e-82 259
MS.gene86828.t1 AT2G01630 42.284 324 181 4 31 349 23 345 8.38e-82 259
MS.gene86828.t1 AT3G13560 42.320 319 178 4 31 344 26 343 4.20e-81 258
MS.gene86828.t1 AT3G13560 42.320 319 178 4 31 344 26 343 4.20e-81 258
MS.gene86828.t1 AT3G13560 42.320 319 178 4 31 344 26 343 4.20e-81 258
MS.gene86828.t1 AT3G13560 42.320 319 178 4 31 344 26 343 4.20e-81 258
MS.gene86828.t1 AT3G13560 42.320 319 178 4 31 344 26 343 4.20e-81 258
MS.gene86828.t1 AT1G11820 40.407 344 199 4 27 365 40 382 9.48e-80 251
MS.gene86828.t1 AT1G11820 40.533 338 195 4 27 359 40 376 3.75e-78 249
MS.gene86828.t1 AT1G11820 40.719 334 192 4 31 359 44 376 8.78e-78 249
MS.gene86828.t1 AT2G39640 39.394 363 210 6 5 366 4 357 2.37e-77 249
MS.gene86828.t1 AT3G55430 39.891 366 201 12 18 374 17 372 4.91e-76 243
MS.gene86828.t1 AT3G46570 44.582 323 167 10 31 347 27 343 1.00e-74 237
MS.gene86828.t1 AT4G16260 37.464 347 206 6 13 356 4 342 1.99e-73 233
MS.gene86828.t1 AT3G61810 37.612 335 194 5 22 349 46 372 1.18e-72 232
MS.gene86828.t1 AT4G34480 51.073 233 113 1 118 349 3 235 1.64e-71 230
MS.gene86828.t1 AT3G57270 40.062 322 182 7 30 348 26 339 3.61e-71 227
MS.gene86828.t1 AT1G66250 37.941 340 204 5 25 359 26 363 9.74e-70 228
MS.gene86828.t1 AT4G16260 37.500 320 191 5 38 356 2 313 7.69e-67 215
MS.gene86828.t1 AT5G24318 47.727 220 109 3 140 357 1 216 5.13e-65 211
MS.gene86828.t1 AT5G20340 38.415 328 189 9 23 346 35 353 2.46e-64 210
MS.gene86828.t1 AT5G20330 36.494 348 204 10 7 346 6 344 2.91e-64 209
MS.gene86828.t1 AT5G20390 36.705 346 206 9 5 346 7 343 5.22e-63 206
MS.gene86828.t1 AT1G77780 36.338 355 206 11 13 360 2 343 2.18e-61 202
MS.gene86828.t1 AT1G33220 36.585 328 187 8 22 346 25 334 3.04e-60 199
MS.gene86828.t1 AT3G57260 34.911 338 206 8 13 348 13 338 6.42e-59 195
MS.gene86828.t1 AT3G57260 34.911 338 206 8 13 348 29 354 1.21e-58 195
MS.gene86828.t1 AT1G77790 33.714 350 219 8 1 346 1 341 6.06e-57 191
MS.gene86828.t1 AT3G57240 36.562 320 189 9 31 349 35 341 3.83e-56 188
MS.gene86828.t1 AT5G58090 35.103 339 202 12 27 355 18 348 5.25e-52 181
MS.gene86828.t1 AT4G31140 32.961 358 219 12 16 364 12 357 1.22e-50 177
MS.gene86828.t1 AT1G64760 33.429 347 213 11 13 349 7 345 8.09e-48 170
MS.gene86828.t1 AT1G64760 33.429 347 213 11 13 349 7 345 8.09e-48 170
MS.gene86828.t1 AT5G18220 32.362 343 208 11 14 344 13 343 3.83e-46 166
MS.gene86828.t1 AT5G20560 33.233 331 209 9 11 338 15 336 6.24e-46 161
MS.gene86828.t1 AT2G19440 32.754 345 213 12 15 349 6 341 3.39e-45 163
MS.gene86828.t1 AT2G19440 32.754 345 213 12 15 349 6 341 3.39e-45 163
MS.gene86828.t1 AT4G17180 32.938 337 207 11 30 358 24 349 3.56e-45 162
MS.gene86828.t1 AT5G64790 32.239 335 211 11 24 349 21 348 1.01e-44 162
MS.gene86828.t1 AT3G24330 32.194 351 216 14 11 349 21 361 1.08e-44 162
MS.gene86828.t1 AT3G55780 33.227 313 196 10 44 349 48 354 2.97e-44 158
MS.gene86828.t1 AT5G20870 33.708 356 200 17 14 346 12 354 1.25e-43 159
MS.gene86828.t1 AT3G04010 33.333 351 204 14 13 348 17 352 3.32e-43 157
MS.gene86828.t1 AT5G58480 31.532 333 210 11 26 348 21 345 6.52e-40 148

Find 80 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTCGTCTTCCGGTTATT+TGG 0.185291 1.1:-58401842 MS.gene86828:CDS
TTATCCTTACTTTGCTTATA+AGG 0.190578 1.1:-58402373 MS.gene86828:CDS
TTGTAACAGTGATTTGTTTA+TGG 0.197294 1.1:+58402529 None:intergenic
CCAACTTCTGAGAGGAATTA+TGG 0.224306 1.1:-58402006 MS.gene86828:CDS
GGGAATCCTGTTTATAATCT+TGG 0.235486 1.1:-58401967 MS.gene86828:CDS
CAAGAAAGGTGAACCAGTTT+TGG 0.236223 1.1:+58402404 None:intergenic
GCGAAAGCTTTGAGAACTTT+TGG 0.247516 1.1:+58402786 None:intergenic
AATTCTTATCCACCTTCTTC+TGG 0.249644 1.1:-58402480 MS.gene86828:CDS
TCCATGCTTGAGCTTGTTTA+GGG 0.266792 1.1:+58402703 None:intergenic
ATCCATGCTTGAGCTTGTTT+AGG 0.272003 1.1:+58402702 None:intergenic
CACACTGCACTCATAGTTCT+TGG 0.288580 1.1:-58402555 MS.gene86828:CDS
GGATCAACCATTCCTTGGTT+TGG 0.327470 1.1:+58402309 None:intergenic
TTAAGCTTTCAATCCAAAAC+TGG 0.338481 1.1:-58402417 MS.gene86828:CDS
TATTCTGCGCTTGGAGCATT+AGG 0.348295 1.1:-58402234 MS.gene86828:CDS
TCGAATGATGGTGGTAATAA+TGG 0.352748 1.1:-58401901 MS.gene86828:CDS
GAAAATGCAAGAAAGTATAA+TGG 0.357716 1.1:-58402132 MS.gene86828:CDS
GTGTGAACACTTTGCATTGC+TGG 0.359796 1.1:+58402573 None:intergenic
TAATCTAACGATATTTGCTT+AGG 0.376376 1.1:+58402345 None:intergenic
CATCCTCCGACTTCGTCTTC+CGG 0.379880 1.1:-58401850 MS.gene86828:CDS
CCGGTGCATATTTCTGAAAC+AGG 0.382721 1.1:-58402198 MS.gene86828:CDS
GGTGGTAATAATGGTACTAT+TGG 0.395208 1.1:-58401892 MS.gene86828:CDS
TAAATATTCAAATCAACTTC+AGG 0.401550 1.1:+58402066 None:intergenic
TTCAGAAATGGCCAAATAAC+CGG 0.409589 1.1:+58401831 None:intergenic
GCAACAAAATCTGAATCAAT+TGG 0.409686 1.1:-58402912 MS.gene86828:CDS
AAAATGCAAGAAAGTATAAT+GGG 0.416433 1.1:-58402131 MS.gene86828:CDS
CAACACCGGTATAGAATTCA+TGG 0.427382 1.1:-58402763 MS.gene86828:CDS
GACGCTGTTTATTCTGCGCT+TGG 0.433976 1.1:-58402243 MS.gene86828:CDS
CATTTCTGAAGCTACTTCAC+TGG 0.441470 1.1:-58401818 MS.gene86828:CDS
TTCCTCTCAGAAGTTGGTCC+AGG 0.441726 1.1:+58402012 None:intergenic
ATAGAATTCATGGTAGGGCT+TGG 0.457900 1.1:-58402753 MS.gene86828:CDS
GAGAATCCAAGATTATAAAC+AGG 0.458638 1.1:+58401961 None:intergenic
AATCTAACGATATTTGCTTA+GGG 0.465599 1.1:+58402346 None:intergenic
GAAACAGGTTGGCCTTCAAA+AGG 0.474545 1.1:-58402183 MS.gene86828:CDS
CAGTGAAGTAGCTTCAGAAA+TGG 0.484958 1.1:+58401819 None:intergenic
TATGGATTGTTTAAGCCTGA+TGG 0.485868 1.1:-58401988 MS.gene86828:CDS
GAAGACGAAGTCGGAGGATG+AGG 0.486816 1.1:+58401853 None:intergenic
TTTGGATTGAAAGCTTAAGA+TGG 0.495529 1.1:+58402422 None:intergenic
ATAAAGTTGAGTAGTAAGAA+AGG 0.495740 1.1:-58402102 MS.gene86828:CDS
TCGAGTCCTCCTTCGAATGA+TGG 0.500599 1.1:-58401913 MS.gene86828:CDS
TTATAAACAGGATTCCCATC+AGG 0.504478 1.1:+58401973 None:intergenic
ATGGATTGTTTAAGCCTGAT+GGG 0.506000 1.1:-58401987 MS.gene86828:CDS
TTCAAAAGGTGACGGGGATG+AGG 0.509119 1.1:-58402169 MS.gene86828:CDS
TGAAGTTGCAATAGCGGTGC+CGG 0.509145 1.1:+58403051 None:intergenic
ACAACATGTTATCATAATGC+AGG 0.509789 1.1:+58402277 None:intergenic
AATAATGGTACTATTGGATC+TGG 0.512155 1.1:-58401886 MS.gene86828:CDS
CCATAATTCCTCTCAGAAGT+TGG 0.512384 1.1:+58402006 None:intergenic
GGATAAGCGTTTATCAAGAA+AGG 0.513672 1.1:+58402390 None:intergenic
CAATAGCGGTGCCGGAATGT+AGG 0.516742 1.1:+58403059 None:intergenic
ACATGGATCATCCTACATTC+CGG 0.525681 1.1:-58403070 MS.gene86828:CDS
GTTTCAACCAAACCAAGGAA+TGG 0.532105 1.1:-58402316 MS.gene86828:CDS
GAACGAAAAGTTCCAGAAGA+AGG 0.534585 1.1:+58402468 None:intergenic
GTTGGCCTTCAAAAGGTGAC+GGG 0.536139 1.1:-58402176 MS.gene86828:CDS
TTATCCTTATAAGCAAAGTA+AGG 0.538288 1.1:+58402369 None:intergenic
TGCATATTTCTGAAACAGGT+TGG 0.538422 1.1:-58402194 MS.gene86828:CDS
CCCTACCATGAATTCTATAC+CGG 0.540456 1.1:+58402758 None:intergenic
CTCAAAGCTTTCGCCAACAC+CGG 0.542007 1.1:-58402777 MS.gene86828:CDS
GTAACCCTAATAAAATGCAT+TGG 0.548583 1.1:-58402840 MS.gene86828:CDS
CATGAATTCTATACCGGTGT+TGG 0.552551 1.1:+58402764 None:intergenic
ACCCTAAACAAGCTCAAGCA+TGG 0.553134 1.1:-58402704 MS.gene86828:CDS
GAATCAATTGGAATAAACTA+CGG 0.568633 1.1:-58402900 MS.gene86828:CDS
AAAGGTGACGGGGATGAGGT+TGG 0.569487 1.1:-58402165 MS.gene86828:CDS
GACGAAGTCGGAGGATGAGG+CGG 0.578289 1.1:+58401856 None:intergenic
CTTAAGATGGAAGCAATGCA+TGG 0.582736 1.1:+58402435 None:intergenic
TTTAATGAGAATTTGAAACC+TGG 0.593849 1.1:-58402030 MS.gene86828:CDS
GGTTGGCCTTCAAAAGGTGA+CGG 0.595397 1.1:-58402177 MS.gene86828:CDS
CGAAAAGTTCCAGAAGAAGG+TGG 0.598625 1.1:+58402471 None:intergenic
CCGGTATAGAATTCATGGTA+GGG 0.598659 1.1:-58402758 MS.gene86828:CDS
ACCGGTATAGAATTCATGGT+AGG 0.606594 1.1:-58402759 MS.gene86828:CDS
ATTAGGTTATGATAAAATGC+CGG 0.621517 1.1:-58402217 MS.gene86828:CDS
CCTGTTTCAGAAATATGCAC+CGG 0.625689 1.1:+58402198 None:intergenic
TTATTACCACCATCATTCGA+AGG 0.630115 1.1:+58401904 None:intergenic
AAATAACCGGAAGACGAAGT+CGG 0.634869 1.1:+58401844 None:intergenic
AATGGTACTATTGGATCTGG+TGG 0.638028 1.1:-58401883 MS.gene86828:CDS
AAATCAACTTCAGGTCTCAA+CGG 0.641716 1.1:+58402075 None:intergenic
AGTCCTCCTTCGAATGATGG+TGG 0.666041 1.1:-58401910 MS.gene86828:CDS
TTACCACCATCATTCGAAGG+AGG 0.670183 1.1:+58401907 None:intergenic
TGATGATGAAGTTGCAATAG+CGG 0.687297 1.1:+58403045 None:intergenic
TAACCGGAAGACGAAGTCGG+AGG 0.687852 1.1:+58401847 None:intergenic
AACCTGGACCAACTTCTGAG+AGG 0.702887 1.1:-58402014 MS.gene86828:CDS
TTGGCCTTCAAAAGGTGACG+GGG 0.711343 1.1:-58402175 MS.gene86828:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATATAATAAGATGTAGT+AGG + chr1.1:58401868-58401887 None:intergenic 20.0%
!! TAAATATTCAAATCAACTTC+AGG + chr1.1:58402788-58402807 None:intergenic 20.0%
!!! AAAATGCAAGAAAGTATAAT+GGG - chr1.1:58402720-58402739 MS.gene86828:CDS 20.0%
! AACTTCATTTCCTACAAAAA+TGG + chr1.1:58402219-58402238 None:intergenic 25.0%
! AATCTAACGATATTTGCTTA+GGG + chr1.1:58402508-58402527 None:intergenic 25.0%
! ATAAAGTTGAGTAGTAAGAA+AGG - chr1.1:58402749-58402768 MS.gene86828:CDS 25.0%
! ATTAGGTTATGATAAAATGC+CGG - chr1.1:58402634-58402653 MS.gene86828:CDS 25.0%
! GAATCAATTGGAATAAACTA+CGG - chr1.1:58401951-58401970 MS.gene86828:CDS 25.0%
! TAATCTAACGATATTTGCTT+AGG + chr1.1:58402509-58402528 None:intergenic 25.0%
! TTATCCTTACTTTGCTTATA+AGG - chr1.1:58402478-58402497 MS.gene86828:CDS 25.0%
! TTATCCTTATAAGCAAAGTA+AGG + chr1.1:58402485-58402504 None:intergenic 25.0%
!! AAAAATGGAAGTGATTTTTG+TGG + chr1.1:58402204-58402223 None:intergenic 25.0%
!! ATCATTAGCATTTTCATTAC+AGG - chr1.1:58401828-58401847 MS.gene86828:CDS 25.0%
!! GAAAATGCAAGAAAGTATAA+TGG - chr1.1:58402719-58402738 MS.gene86828:CDS 25.0%
!! TTTAATGAGAATTTGAAACC+TGG - chr1.1:58402821-58402840 MS.gene86828:CDS 25.0%
!!! AAAATCACTTCCATTTTTGT+AGG - chr1.1:58402206-58402225 MS.gene86828:CDS 25.0%
!!! TTGTAACAGTGATTTGTTTA+TGG + chr1.1:58402325-58402344 None:intergenic 25.0%
AATAATGGTACTATTGGATC+TGG - chr1.1:58402965-58402984 MS.gene86828:intron 30.0%
ACAACATGTTATCATAATGC+AGG + chr1.1:58402577-58402596 None:intergenic 30.0%
GAGAATCCAAGATTATAAAC+AGG + chr1.1:58402893-58402912 None:intergenic 30.0%
GCAACAAAATCTGAATCAAT+TGG - chr1.1:58401939-58401958 MS.gene86828:CDS 30.0%
GTAACCCTAATAAAATGCAT+TGG - chr1.1:58402011-58402030 MS.gene86828:CDS 30.0%
TTAAGCTTTCAATCCAAAAC+TGG - chr1.1:58402434-58402453 MS.gene86828:CDS 30.0%
TTTGGATTGAAAGCTTAAGA+TGG + chr1.1:58402432-58402451 None:intergenic 30.0%
! TGCTCCAATGCATTTTATTA+GGG + chr1.1:58402018-58402037 None:intergenic 30.0%
! TTGCTCCAATGCATTTTATT+AGG + chr1.1:58402019-58402038 None:intergenic 30.0%
!! TCATAATGCAGGTTTGTTTT+TGG + chr1.1:58402566-58402585 None:intergenic 30.0%
AAATCAACTTCAGGTCTCAA+CGG + chr1.1:58402779-58402798 None:intergenic 35.0%
AATTCTTATCCACCTTCTTC+TGG - chr1.1:58402371-58402390 MS.gene86828:CDS 35.0%
GGATAAGCGTTTATCAAGAA+AGG + chr1.1:58402464-58402483 None:intergenic 35.0%
GGGAATCCTGTTTATAATCT+TGG - chr1.1:58402884-58402903 MS.gene86828:CDS 35.0%
GGTGGTAATAATGGTACTAT+TGG - chr1.1:58402959-58402978 MS.gene86828:intron 35.0%
TTATAAACAGGATTCCCATC+AGG + chr1.1:58402881-58402900 None:intergenic 35.0%
TTATTACCACCATCATTCGA+AGG + chr1.1:58402950-58402969 None:intergenic 35.0%
TTCAGAAATGGCCAAATAAC+CGG + chr1.1:58403023-58403042 None:intergenic 35.0%
! ATGGAAGTGATTTTTGTGGA+AGG + chr1.1:58402200-58402219 None:intergenic 35.0%
! ATGGATTGTTTAAGCCTGAT+GGG - chr1.1:58402864-58402883 MS.gene86828:CDS 35.0%
! ATTAGGGTTACTACGTTTTC+TGG + chr1.1:58402002-58402021 None:intergenic 35.0%
! TATGGATTGTTTAAGCCTGA+TGG - chr1.1:58402863-58402882 MS.gene86828:CDS 35.0%
! TGATGATGAAGTTGCAATAG+CGG + chr1.1:58401809-58401828 None:intergenic 35.0%
! TGGAAGTGATTTTTGTGGAA+GGG + chr1.1:58402199-58402218 None:intergenic 35.0%
! TTTTTGGATCAACCATTCCT+TGG + chr1.1:58402550-58402569 None:intergenic 35.0%
!! TCGAATGATGGTGGTAATAA+TGG - chr1.1:58402950-58402969 MS.gene86828:intron 35.0%
!! TGCATATTTCTGAAACAGGT+TGG - chr1.1:58402657-58402676 MS.gene86828:CDS 35.0%
!!! GTTTTGTTTCAACCAAACCA+AGG - chr1.1:58402530-58402549 MS.gene86828:CDS 35.0%
AAACAGTGTCCTATTTGTGC+AGG - chr1.1:58401912-58401931 MS.gene86828:CDS 40.0%
AAATAACCGGAAGACGAAGT+CGG + chr1.1:58403010-58403029 None:intergenic 40.0%
AATGGTACTATTGGATCTGG+TGG - chr1.1:58402968-58402987 MS.gene86828:intron 40.0%
ACCGGTATAGAATTCATGGT+AGG - chr1.1:58402092-58402111 MS.gene86828:CDS 40.0%
ATAGAATTCATGGTAGGGCT+TGG - chr1.1:58402098-58402117 MS.gene86828:CDS 40.0%
CAACACCGGTATAGAATTCA+TGG - chr1.1:58402088-58402107 MS.gene86828:CDS 40.0%
CAGTGAAGTAGCTTCAGAAA+TGG + chr1.1:58403035-58403054 None:intergenic 40.0%
CATGAATTCTATACCGGTGT+TGG + chr1.1:58402090-58402109 None:intergenic 40.0%
CCATAATTCCTCTCAGAAGT+TGG + chr1.1:58402848-58402867 None:intergenic 40.0%
CCCTACCATGAATTCTATAC+CGG + chr1.1:58402096-58402115 None:intergenic 40.0%
CCGGTATAGAATTCATGGTA+GGG - chr1.1:58402093-58402112 MS.gene86828:CDS 40.0%
CCTGTTTCAGAAATATGCAC+CGG + chr1.1:58402656-58402675 None:intergenic 40.0%
CTTAAGATGGAAGCAATGCA+TGG + chr1.1:58402419-58402438 None:intergenic 40.0%
GAACGAAAAGTTCCAGAAGA+AGG + chr1.1:58402386-58402405 None:intergenic 40.0%
GTTTCAACCAAACCAAGGAA+TGG - chr1.1:58402535-58402554 MS.gene86828:CDS 40.0%
! AAGTGATTTTTGTGGAAGGG+AGG + chr1.1:58402196-58402215 None:intergenic 40.0%
! ATCCATGCTTGAGCTTGTTT+AGG + chr1.1:58402152-58402171 None:intergenic 40.0%
! ATTTTTGTGGAAGGGAGGTA+GGG + chr1.1:58402191-58402210 None:intergenic 40.0%
! CAAGAAAGGTGAACCAGTTT+TGG + chr1.1:58402450-58402469 None:intergenic 40.0%
! CCAACTTCTGAGAGGAATTA+TGG - chr1.1:58402845-58402864 MS.gene86828:CDS 40.0%
! TCCATGCTTGAGCTTGTTTA+GGG + chr1.1:58402151-58402170 None:intergenic 40.0%
!! CATTTCTGAAGCTACTTCAC+TGG - chr1.1:58403033-58403052 MS.gene86828:CDS 40.0%
!! GTTACTACGTTTTCTGGTGA+TGG + chr1.1:58401996-58402015 None:intergenic 40.0%
!!! GCGAAAGCTTTGAGAACTTT+TGG + chr1.1:58402068-58402087 None:intergenic 40.0%
ACCCTAAACAAGCTCAAGCA+TGG - chr1.1:58402147-58402166 MS.gene86828:CDS 45.0%
CACACTGCACTCATAGTTCT+TGG - chr1.1:58402296-58402315 MS.gene86828:CDS 45.0%
CGAAAAGTTCCAGAAGAAGG+TGG + chr1.1:58402383-58402402 None:intergenic 45.0%
GACTTCGTCTTCCGGTTATT+TGG - chr1.1:58403009-58403028 MS.gene86828:intron 45.0%
GGATCAACCATTCCTTGGTT+TGG + chr1.1:58402545-58402564 None:intergenic 45.0%
GTGTGAACACTTTGCATTGC+TGG + chr1.1:58402281-58402300 None:intergenic 45.0%
GTTGCTAGTCCTGCACAAAT+AGG + chr1.1:58401924-58401943 None:intergenic 45.0%
TTACCACCATCATTCGAAGG+AGG + chr1.1:58402947-58402966 None:intergenic 45.0%
! GATTTTTGTGGAAGGGAGGT+AGG + chr1.1:58402192-58402211 None:intergenic 45.0%
! TATTCTGCGCTTGGAGCATT+AGG - chr1.1:58402617-58402636 MS.gene86828:CDS 45.0%
!! CCGGTGCATATTTCTGAAAC+AGG - chr1.1:58402653-58402672 MS.gene86828:CDS 45.0%
!! GAAACAGGTTGGCCTTCAAA+AGG - chr1.1:58402668-58402687 MS.gene86828:CDS 45.0%
!!! CTACGTTTTCTGGTGATGGT+AGG + chr1.1:58401992-58402011 None:intergenic 45.0%
AACCTGGACCAACTTCTGAG+AGG - chr1.1:58402837-58402856 MS.gene86828:CDS 50.0%
AGTCCTCCTTCGAATGATGG+TGG - chr1.1:58402941-58402960 MS.gene86828:intron 50.0%
CTCAAAGCTTTCGCCAACAC+CGG - chr1.1:58402074-58402093 MS.gene86828:CDS 50.0%
GACGCTGTTTATTCTGCGCT+TGG - chr1.1:58402608-58402627 MS.gene86828:CDS 50.0%
TCGAGTCCTCCTTCGAATGA+TGG - chr1.1:58402938-58402957 MS.gene86828:intron 50.0%
TGAAGTTGCAATAGCGGTGC+CGG + chr1.1:58401803-58401822 None:intergenic 50.0%
! TCATCCCCGTCACCTTTTGA+AGG + chr1.1:58402683-58402702 None:intergenic 50.0%
! TTCCTCTCAGAAGTTGGTCC+AGG + chr1.1:58402842-58402861 None:intergenic 50.0%
! TTGGCCTTCAAAAGGTGACG+GGG - chr1.1:58402676-58402695 MS.gene86828:CDS 50.0%
!! GGTTGGCCTTCAAAAGGTGA+CGG - chr1.1:58402674-58402693 MS.gene86828:CDS 50.0%
!! GTTGGCCTTCAAAAGGTGAC+GGG - chr1.1:58402675-58402694 MS.gene86828:CDS 50.0%
!! TTCAAAAGGTGACGGGGATG+AGG - chr1.1:58402682-58402701 MS.gene86828:CDS 50.0%
CATCCTCCGACTTCGTCTTC+CGG - chr1.1:58403001-58403020 MS.gene86828:intron 55.0%
GAAGACGAAGTCGGAGGATG+AGG + chr1.1:58403001-58403020 None:intergenic 55.0%
TAACCGGAAGACGAAGTCGG+AGG + chr1.1:58403007-58403026 None:intergenic 55.0%
! CAATAGCGGTGCCGGAATGT+AGG + chr1.1:58401795-58401814 None:intergenic 55.0%
!! AAAGGTGACGGGGATGAGGT+TGG - chr1.1:58402686-58402705 MS.gene86828:CDS 55.0%
GACGAAGTCGGAGGATGAGG+CGG + chr1.1:58402998-58403017 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 58401783 58403090 58401783 ID=MS.gene86828
chr1.1 mRNA 58401783 58403090 58401783 ID=MS.gene86828.t1;Parent=MS.gene86828
chr1.1 exon 58403024 58403090 58403024 ID=MS.gene86828.t1.exon1;Parent=MS.gene86828.t1
chr1.1 CDS 58403024 58403090 58403024 ID=cds.MS.gene86828.t1;Parent=MS.gene86828.t1
chr1.1 exon 58401783 58402939 58401783 ID=MS.gene86828.t1.exon2;Parent=MS.gene86828.t1
chr1.1 CDS 58401783 58402939 58401783 ID=cds.MS.gene86828.t1;Parent=MS.gene86828.t1
Gene Sequence

>MS.gene86828

ATGGATCATCCTACATTCCGGCACCGCTATTGCAACTTCATCATCATCATTAGCATTTTCATTACAGGTAAGAATTTCTACTCCTACTACATCTTATTATATAGTCTTAAAAATGTAACATATCACTGAAAACAGTGTCCTATTTGTGCAGGACTAGCAACAAAATCTGAATCAATTGGAATAAACTACGGTCAAATAGCAAACAACCTACCATCACCAGAAAACGTAGTAACCCTAATAAAATGCATTGGAGCAACAAAAGTAAAACTCTACGACGCAGATCCAAAAGTTCTCAAAGCTTTCGCCAACACCGGTATAGAATTCATGGTAGGGCTTGGAAATGAATATCTCTCTAAAATGAAAGACCCTAAACAAGCTCAAGCATGGATCAAAACAAATCTTCAACCCTACCTCCCTTCCACAAAAATCACTTCCATTTTTGTAGGAAATGAAGTTCTCACTTTCAACGACTCTTCTTTAACATCTTCTCTTCTACCAGCAATGCAAAGTGTTCACACTGCACTCATAGTTCTTGGTCTCCATAAACAAATCACTGTTACAACAACTCATTCATTAGCTATACTACAAAATTCTTATCCACCTTCTTCTGGAACTTTTCGTTCTGATATAGCTCCATGCATTGCTTCCATCTTAAGCTTTCAATCCAAAACTGGTTCACCTTTCTTGATAAACGCTTATCCTTACTTTGCTTATAAGGATAACCCTAAGCAAATATCGTTAGATTATGTTTTGTTTCAACCAAACCAAGGAATGGTTGATCCAAAAACAAACCTGCATTATGATAACATGTTGTTTGCGCAGATCGACGCTGTTTATTCTGCGCTTGGAGCATTAGGTTATGATAAAATGCCGGTGCATATTTCTGAAACAGGTTGGCCTTCAAAAGGTGACGGGGATGAGGTTGGTGCTAATGTTGAAAATGCAAGAAAGTATAATGGGAATGTGATAAAGTTGAGTAGTAAGAAAGGAACACCGTTGAGACCTGAAGTTGATTTGAATATTTATGTTTTTGCACTTTTTAATGAGAATTTGAAACCTGGACCAACTTCTGAGAGGAATTATGGATTGTTTAAGCCTGATGGGAATCCTGTTTATAATCTTGGATTCTCGCTGTCTTCGTCTTCTTCTTCGAATTCGAGTCCTCCTTCGAATGATGGTGGTAATAATGGTACTATTGGATCTGGTGGTGCTCCGCCTCATCCTCCGACTTCGTCTTCCGGTTATTTGGCCATTTCTGAAGCTACTTCACTGGTTAGTAACACTCACACTGCTGCTTCTAAGTTTTGA
Protein sequence

>MS.gene86828.t1

MDHPTFRHRYCNFIIIISIFITGLATKSESIGINYGQIANNLPSPENVVTLIKCIGATKVKLYDADPKVLKAFANTGIEFMVGLGNEYLSKMKDPKQAQAWIKTNLQPYLPSTKITSIFVGNEVLTFNDSSLTSSLLPAMQSVHTALIVLGLHKQITVTTTHSLAILQNSYPPSSGTFRSDIAPCIASILSFQSKTGSPFLINAYPYFAYKDNPKQISLDYVLFQPNQGMVDPKTNLHYDNMLFAQIDAVYSALGALGYDKMPVHISETGWPSKGDGDEVGANVENARKYNGNVIKLSSKKGTPLRPEVDLNIYVFALFNENLKPGPTSERNYGLFKPDGNPVYNLGFSLSSSSSSNSSPPSNDGGNNGTIGSGGAPPHPPTSSSGYLAISEATSLVSNTHTAASKF