Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87513.t1 | RHN59155.1 | 97.7 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 1.10E-39 | 172.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87513.t1 | G7JUK2 | 97.7 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 7.9e-40 | 172.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051800 | MS.gene87513 | 0.809723 | 1.64E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene87513 | 0.811364 | 7.27E-51 | -1.69E-46 |
| MS.gene05417 | MS.gene87513 | 0.821418 | 4.08E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene87513 | 0.810929 | 9.03E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene87513 | 0.810579 | 1.07E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene87513 | 0.804 | 2.66E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene87513 | 0.801055 | 1.08E-48 | -1.69E-46 |
| MS.gene057869 | MS.gene87513 | 0.806751 | 7.07E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene87513 | 0.80556 | 1.26E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene87513 | 0.810045 | 1.40E-50 | -1.69E-46 |
| MS.gene059584 | MS.gene87513 | 0.805712 | 1.17E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene87513 | 0.826896 | 2.11E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87513.t1 | MTR_4g022460 | 97.727 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 4.00e-52 | 174 |
| MS.gene87513.t1 | MTR_4g022560 | 89.773 | 88 | 9 | 0 | 1 | 88 | 1 | 88 | 5.25e-41 | 142 |
| MS.gene87513.t1 | MTR_4g022540 | 92.045 | 88 | 7 | 0 | 1 | 88 | 1 | 88 | 1.57e-39 | 138 |
| MS.gene87513.t1 | MTR_4g022960 | 90.909 | 88 | 8 | 0 | 1 | 88 | 1 | 88 | 8.55e-39 | 136 |
| MS.gene87513.t1 | MTR_4g022480 | 93.182 | 88 | 6 | 0 | 1 | 88 | 1 | 88 | 2.59e-37 | 132 |
| MS.gene87513.t1 | MTR_4g021690 | 93.182 | 88 | 6 | 0 | 1 | 88 | 1 | 88 | 2.88e-37 | 132 |
| MS.gene87513.t1 | MTR_4g022940 | 74.074 | 54 | 14 | 0 | 1 | 54 | 1 | 54 | 7.68e-24 | 86.3 |
| MS.gene87513.t1 | MTR_3g015500 | 48.837 | 86 | 42 | 1 | 3 | 88 | 40 | 123 | 1.88e-18 | 78.6 |
| MS.gene87513.t1 | MTR_4g050790 | 47.674 | 86 | 43 | 1 | 3 | 88 | 2 | 85 | 2.65e-17 | 75.1 |
| MS.gene87513.t1 | MTR_4g119195 | 42.391 | 92 | 48 | 2 | 1 | 88 | 1 | 91 | 1.71e-15 | 70.1 |
| MS.gene87513.t1 | MTR_4g119195 | 42.391 | 92 | 48 | 2 | 1 | 88 | 1 | 91 | 1.86e-15 | 70.1 |
| MS.gene87513.t1 | MTR_5g076840 | 43.478 | 92 | 47 | 2 | 1 | 88 | 1 | 91 | 2.30e-15 | 69.7 |
| MS.gene87513.t1 | MTR_8g027540 | 40.000 | 90 | 50 | 1 | 1 | 86 | 1 | 90 | 1.78e-14 | 67.0 |
| MS.gene87513.t1 | MTR_7g078640 | 40.217 | 92 | 50 | 2 | 1 | 88 | 1 | 91 | 3.45e-14 | 66.2 |
| MS.gene87513.t1 | MTR_7g078300 | 40.217 | 92 | 50 | 2 | 1 | 88 | 1 | 91 | 3.56e-14 | 66.2 |
| MS.gene87513.t1 | MTR_6g037690 | 40.217 | 92 | 50 | 2 | 1 | 88 | 1 | 91 | 1.20e-13 | 64.7 |
| MS.gene87513.t1 | MTR_8g011550 | 38.710 | 93 | 51 | 3 | 1 | 88 | 1 | 92 | 1.77e-13 | 64.3 |
| MS.gene87513.t1 | MTR_8g011180 | 38.043 | 92 | 52 | 2 | 1 | 88 | 1 | 91 | 3.30e-13 | 63.5 |
| MS.gene87513.t1 | MTR_1g078490 | 39.130 | 92 | 51 | 2 | 1 | 88 | 1 | 91 | 6.96e-13 | 62.8 |
| MS.gene87513.t1 | MTR_8g011170 | 38.043 | 92 | 52 | 2 | 1 | 88 | 1 | 91 | 7.18e-13 | 62.8 |
| MS.gene87513.t1 | MTR_6g452490 | 38.202 | 89 | 50 | 2 | 1 | 85 | 1 | 88 | 2.17e-12 | 61.2 |
| MS.gene87513.t1 | MTR_8g445980 | 43.902 | 82 | 41 | 2 | 11 | 88 | 13 | 93 | 2.26e-12 | 61.2 |
| MS.gene87513.t1 | MTR_8g011300 | 37.079 | 89 | 51 | 2 | 1 | 85 | 1 | 88 | 8.42e-12 | 59.7 |
| MS.gene87513.t1 | MTR_7g491180 | 35.870 | 92 | 54 | 2 | 1 | 88 | 1 | 91 | 1.35e-11 | 58.9 |
| MS.gene87513.t1 | MTR_7g091190 | 35.870 | 92 | 54 | 2 | 1 | 88 | 1 | 91 | 1.35e-11 | 58.9 |
| MS.gene87513.t1 | MTR_1g030770 | 39.130 | 92 | 51 | 2 | 1 | 88 | 1 | 91 | 1.99e-11 | 58.5 |
| MS.gene87513.t1 | MTR_3g015550 | 48.864 | 88 | 43 | 1 | 1 | 88 | 37 | 122 | 2.81e-11 | 58.2 |
| MS.gene87513.t1 | MTR_8g010440 | 38.202 | 89 | 50 | 2 | 1 | 85 | 1 | 88 | 3.50e-11 | 57.8 |
| MS.gene87513.t1 | MTR_3g015550 | 50.000 | 86 | 41 | 1 | 3 | 88 | 2 | 85 | 3.62e-11 | 57.8 |
| MS.gene87513.t1 | MTR_4g094540 | 36.782 | 87 | 54 | 1 | 2 | 88 | 5 | 90 | 3.80e-11 | 57.8 |
| MS.gene87513.t1 | MTR_3g015560 | 48.837 | 86 | 42 | 1 | 3 | 88 | 2 | 85 | 7.23e-11 | 57.0 |
| MS.gene87513.t1 | MTR_8g043600 | 52.632 | 57 | 26 | 1 | 31 | 86 | 13 | 69 | 8.58e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGATCAAGGCTGTGGTTT+TGG | 0.283133 | 4.1:-75898473 | MS.gene87513:CDS |
| CCAGCCAGAGTTGAACTTGA+TGG | 0.301091 | 4.1:+75898422 | None:intergenic |
| CACTTGTCGCCTCAAGTCTT+CGG | 0.432677 | 4.1:+75898345 | None:intergenic |
| GTTCTGTGGTTGTTGAATGC+TGG | 0.450945 | 4.1:-75898537 | MS.gene87513:CDS |
| GAGTTGAACTTGATGGTTGT+TGG | 0.452378 | 4.1:+75898429 | None:intergenic |
| TTTGGATGCAGAGGAGCAGC+AGG | 0.459111 | 4.1:-75898455 | MS.gene87513:CDS |
| ACATGATTGAGAAGCTGATC+GGG | 0.460820 | 4.1:-75898567 | MS.gene87513:CDS |
| CCATCAAGTTCAACTCTGGC+TGG | 0.489227 | 4.1:-75898422 | MS.gene87513:CDS |
| AACATGATTGAGAAGCTGAT+CGG | 0.554548 | 4.1:-75898568 | MS.gene87513:CDS |
| TGACAATATGTCTGAGATCA+AGG | 0.576449 | 4.1:-75898485 | MS.gene87513:CDS |
| GAGAGATGATCTTGACAAGC+TGG | 0.576674 | 4.1:-75898509 | MS.gene87513:CDS |
| GAAGTCATCGAGCAAGTCAT+CGG | 0.584047 | 4.1:+75898372 | None:intergenic |
| ACAACCATCAAGTTCAACTC+TGG | 0.584621 | 4.1:-75898426 | MS.gene87513:CDS |
| TATGTCTGAGATCAAGGCTG+TGG | 0.592076 | 4.1:-75898479 | MS.gene87513:CDS |
| GATCGGGAAGCTGAGTTCTG+TGG | 0.611586 | 4.1:-75898551 | MS.gene87513:CDS |
| ACTTCAACACCGAAGACTTG+AGG | 0.622617 | 4.1:-75898354 | MS.gene87513:CDS |
| TTGGATGCAGAGGAGCAGCA+GGG | 0.680480 | 4.1:-75898454 | MS.gene87513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATGATTGAGAAGCTGAT+CGG | - | chr4.1:75898369-75898388 | MS.gene87513:CDS | 35.0% | |
| ! | TGACAATATGTCTGAGATCA+AGG | - | chr4.1:75898452-75898471 | MS.gene87513:CDS | 35.0% |
| ACAACCATCAAGTTCAACTC+TGG | - | chr4.1:75898511-75898530 | MS.gene87513:CDS | 40.0% | |
| ACATGATTGAGAAGCTGATC+GGG | - | chr4.1:75898370-75898389 | MS.gene87513:CDS | 40.0% | |
| ! | GAGTTGAACTTGATGGTTGT+TGG | + | chr4.1:75898511-75898530 | None:intergenic | 40.0% |
| ACTTCAACACCGAAGACTTG+AGG | - | chr4.1:75898583-75898602 | MS.gene87513:CDS | 45.0% | |
| GAAGTCATCGAGCAAGTCAT+CGG | + | chr4.1:75898568-75898587 | None:intergenic | 45.0% | |
| GAGAGATGATCTTGACAAGC+TGG | - | chr4.1:75898428-75898447 | MS.gene87513:CDS | 45.0% | |
| GTTCTGTGGTTGTTGAATGC+TGG | - | chr4.1:75898400-75898419 | MS.gene87513:CDS | 45.0% | |
| ! | TATGTCTGAGATCAAGGCTG+TGG | - | chr4.1:75898458-75898477 | MS.gene87513:CDS | 45.0% |
| ! | TGAGATCAAGGCTGTGGTTT+TGG | - | chr4.1:75898464-75898483 | MS.gene87513:CDS | 45.0% |
| CCAGCCAGAGTTGAACTTGA+TGG | + | chr4.1:75898518-75898537 | None:intergenic | 50.0% | |
| CCATCAAGTTCAACTCTGGC+TGG | - | chr4.1:75898515-75898534 | MS.gene87513:CDS | 50.0% | |
| GATCGGGAAGCTGAGTTCTG+TGG | - | chr4.1:75898386-75898405 | MS.gene87513:CDS | 55.0% | |
| TTGGATGCAGAGGAGCAGCA+GGG | - | chr4.1:75898483-75898502 | MS.gene87513:CDS | 55.0% | |
| TTTGGATGCAGAGGAGCAGC+AGG | - | chr4.1:75898482-75898501 | MS.gene87513:CDS | 55.0% | |
| !!! | GGCTGTGGTTTTGGATGCAG+AGG | - | chr4.1:75898473-75898492 | MS.gene87513:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 75898348 | 75898611 | 75898348 | ID=MS.gene87513 |
| chr4.1 | mRNA | 75898348 | 75898611 | 75898348 | ID=MS.gene87513.t1;Parent=MS.gene87513 |
| chr4.1 | exon | 75898348 | 75898611 | 75898348 | ID=MS.gene87513.t1.exon1;Parent=MS.gene87513.t1 |
| chr4.1 | CDS | 75898348 | 75898611 | 75898348 | ID=cds.MS.gene87513.t1;Parent=MS.gene87513.t1 |
| Gene Sequence |
| Protein sequence |
