AlfalfaGEDB | MS.gene87985

M. sativa cultivar XinJiangDaYe / MS.gene87985

KEGG: K15397
GO: GO:0003674 GO:0003824 GO:0005575 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016020 GO:0016053 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0071704 GO:0072330 GO:0110165 GO:1901576
PFAM: PF08392.15 PF08541.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87985.t1	RHN50816.1	97.8	226	5	0	1	226	40	265	1.30E-124	455.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87985.t1	O48780	63.3	218	80	0	1	218	287	504	4.1e-79	295.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87985.t1	A0A072U924	97.8	226	5	0	1	226	168	393	9.2e-125	455.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene87985	0.819336	1.23E-52	-1.69E-46
MS.gene050563	MS.gene87985	0.812127	4.96E-51	-1.69E-46
MS.gene050584	MS.gene87985	0.802955	4.39E-49	-1.69E-46
MS.gene050587	MS.gene87985	0.806857	6.71E-50	-1.69E-46
MS.gene051789	MS.gene87985	0.80199	6.94E-49	-1.69E-46
MS.gene052142	MS.gene87985	0.833239	5.98E-56	-1.69E-46
MS.gene053383	MS.gene87985	0.83262	8.52E-56	-1.69E-46
MS.gene05456	MS.gene87985	0.839752	1.31E-57	-1.69E-46
MS.gene055359	MS.gene87985	0.813842	2.09E-51	-1.69E-46
MS.gene059006	MS.gene87985	0.819828	9.46E-53	-1.69E-46
MS.gene059768	MS.gene87985	0.811663	6.26E-51	-1.69E-46
MS.gene060691	MS.gene87985	0.816709	4.81E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87985.t1	MTR_6g032965	97.788	226	5	0	1	226	168	393	9.08e-168	468
MS.gene87985.t1	MTR_1g076430	66.972	218	72	0	1	218	290	507	5.89e-108	320
MS.gene87985.t1	MTR_1g041085	66.055	218	74	0	1	218	296	513	3.90e-107	318
MS.gene87985.t1	MTR_2g054940	63.761	218	79	0	1	218	289	506	3.47e-102	305
MS.gene87985.t1	MTR_4g113280	61.468	218	84	0	1	218	267	484	5.64e-99	296
MS.gene87985.t1	MTR_7g081695	63.551	214	78	0	1	214	277	490	1.06e-96	298
MS.gene87985.t1	MTR_4g054415	60.455	220	86	1	1	219	208	427	3.58e-96	287
MS.gene87985.t1	MTR_4g054630	60.455	220	86	1	1	219	249	468	6.81e-96	288
MS.gene87985.t1	MTR_7g118170	59.193	223	88	2	1	220	309	531	4.62e-95	288
MS.gene87985.t1	MTR_6g074880	59.259	216	87	1	1	215	251	466	2.50e-90	273
MS.gene87985.t1	MTR_4g070270	57.143	217	92	1	1	217	287	502	9.12e-89	271
MS.gene87985.t1	MTR_7g118160	55.605	223	92	2	1	221	206	423	2.44e-87	265
MS.gene87985.t1	MTR_1g110930	53.211	218	101	1	1	218	275	491	8.66e-86	263
MS.gene87985.t1	MTR_5g056510	52.752	218	102	1	1	218	275	491	2.09e-84	259
MS.gene87985.t1	MTR_2g007020	55.455	220	96	2	1	219	235	453	3.31e-84	258
MS.gene87985.t1	MTR_3g105550	52.655	226	98	1	1	217	311	536	4.10e-82	254
MS.gene87985.t1	MTR_4g054260	55.093	216	79	2	1	215	125	323	3.66e-79	240
MS.gene87985.t1	MTR_2g436480	50.685	219	105	2	1	217	234	451	1.86e-74	232
MS.gene87985.t1	MTR_4g053905	60.870	161	63	0	59	219	1	161	1.34e-71	215
MS.gene87985.t1	MTR_2g013740	48.571	210	102	3	1	209	299	503	1.90e-61	200
MS.gene87985.t1	MTR_4g062190	40.833	240	120	3	2	225	220	453	1.01e-54	182
MS.gene87985.t1	MTR_4g062270	41.667	228	126	2	2	225	220	444	1.32e-54	181
MS.gene87985.t1	MTR_1g102290	39.815	216	129	1	1	215	269	484	1.95e-50	171
MS.gene87985.t1	MTR_4g062170	39.496	238	125	3	2	225	220	452	5.24e-50	169
MS.gene87985.t1	MTR_2g096210	39.394	231	116	4	2	214	220	444	1.98e-49	168
MS.gene87985.t1	MTR_6g051440	41.284	218	121	3	1	215	269	482	1.15e-47	163
MS.gene87985.t1	MTR_1g115560	35.897	234	126	4	2	214	221	451	8.87e-46	158
MS.gene87985.t1	MTR_3g072940	40.826	218	122	3	1	215	270	483	2.44e-45	157
MS.gene87985.t1	MTR_3g072980	40.826	218	122	3	1	215	270	483	2.54e-45	157

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87985.t1	AT2G26640	63.303	218	80	0	1	218	287	504	6.31e-102	305
MS.gene87985.t1	AT1G04220	61.333	225	80	3	1	218	292	516	2.58e-101	303
MS.gene87985.t1	AT1G01120	60.185	216	85	1	1	215	309	524	3.18e-97	293
MS.gene87985.t1	AT5G43760	63.393	224	76	3	1	218	298	521	2.22e-96	291
MS.gene87985.t1	AT4G34510	58.636	220	89	2	1	219	269	487	1.59e-93	282
MS.gene87985.t1	AT2G16280	58.525	217	89	1	1	217	295	510	1.11e-92	281
MS.gene87985.t1	AT1G19440	56.221	217	94	1	1	217	299	514	8.42e-91	276
MS.gene87985.t1	AT1G25450	55.505	218	96	1	1	218	271	487	6.89e-87	265
MS.gene87985.t1	AT3G10280	54.378	217	97	1	1	215	240	456	9.85e-86	261
MS.gene87985.t1	AT2G46720	54.378	217	97	1	1	215	247	463	1.13e-85	261
MS.gene87985.t1	AT4G34250	54.545	220	99	1	1	220	272	490	7.17e-85	260
MS.gene87985.t1	AT1G68530	54.338	219	97	2	1	218	276	492	4.42e-84	258
MS.gene87985.t1	AT2G15090	52.995	217	101	1	1	217	264	479	7.74e-84	257
MS.gene87985.t1	AT4G34520	55.963	218	94	2	1	217	274	490	5.48e-83	256
MS.gene87985.t1	AT1G71160	53.488	215	98	2	1	215	234	446	4.02e-77	239
MS.gene87985.t1	AT5G49070	50.000	220	107	2	1	218	238	456	1.56e-75	235
MS.gene87985.t1	AT2G26250	45.267	243	107	1	1	217	308	550	3.01e-71	227
MS.gene87985.t1	AT2G28630	38.235	238	133	3	2	225	220	457	1.94e-52	176
MS.gene87985.t1	AT2G28630	38.235	238	133	3	2	225	220	457	1.94e-52	176
MS.gene87985.t1	AT5G04530	40.000	225	124	3	2	218	224	445	2.25e-50	170
MS.gene87985.t1	AT3G52160	41.748	206	89	2	1	206	162	336	5.52e-50	166
MS.gene87985.t1	AT3G52160	41.748	206	89	2	1	206	275	449	5.26e-49	166
MS.gene87985.t1	AT1G07720	37.719	228	127	3	2	214	220	447	2.34e-48	165
MS.gene87985.t1	AT1G07720	37.719	228	127	3	2	214	263	490	3.91e-48	166
MS.gene87985.t1	AT1G68530	50.000	74	37	0	1	74	276	349	3.92e-18	82.4
MS.gene87985.t1	AT5G49070	44.737	76	42	0	1	76	238	313	1.12e-16	77.8

Find 32 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGGCAAATGGCTTT+TGG	0.246325	6.1:+11548193	MS.gene87985:CDS
TCCATTAGTCCTCCCAATTT+CGG	0.294314	6.1:+11547895	MS.gene87985:CDS
TCCGAAATTGGGAGGACTAA+TGG	0.324601	6.1:-11547896	None:intergenic
AGCTAAGGGGAGAGTTAAAA+AGG	0.330815	6.1:+11548165	MS.gene87985:CDS
TTGTTCATTGGCAATGGAAC+AGG	0.342492	6.1:-11548313	None:intergenic
AACTTAAAATTCGGCATGTA+TGG	0.357301	6.1:-11547977	None:intergenic
TTAATCATATGTTTGTTCAT+TGG	0.363214	6.1:-11548325	None:intergenic
TCGAACGCTAACTTAAAATT+CGG	0.417831	6.1:-11547986	None:intergenic
TTAAGGTTAATACTGCTGTT+TGG	0.420779	6.1:+11548224	MS.gene87985:CDS
CTATATAGTGTCATTCTTGA+AGG	0.424575	6.1:-11548091	None:intergenic
GGGACATAAAATATGGCAAA+TGG	0.457927	6.1:+11548186	MS.gene87985:CDS
ACACATCATCAAGTTCTGTT+TGG	0.465892	6.1:+11548122	MS.gene87985:CDS
CGATGGATGACTTGAGAGTA+GGG	0.466110	6.1:-11547690	None:intergenic
AGAATGACACTATATAGATA+TGG	0.470646	6.1:+11548097	MS.gene87985:CDS
ATATGTTTGTTCATTGGCAA+TGG	0.471516	6.1:-11548319	None:intergenic
ATTGTCTTATTGTGAAGCTA+AGG	0.483820	6.1:+11548150	MS.gene87985:CDS
TTTGAGCGGCTACGGTCCGA+TGG	0.522197	6.1:-11547707	None:intergenic
TTGTCTTATTGTGAAGCTAA+GGG	0.526933	6.1:+11548151	MS.gene87985:CDS
CCGATGGATGACTTGAGAGT+AGG	0.528757	6.1:-11547691	None:intergenic
AGGATCTCATGAATGTTGCT+AGG	0.531519	6.1:+11547840	MS.gene87985:CDS
GCGGTAGAGCGGTTCTTGAT+CGG	0.540567	6.1:+11548029	MS.gene87985:CDS
AATTGTCTCTTTCGAATGGG+TGG	0.541404	6.1:+11547659	None:intergenic
GGATCTCATGAATGTTGCTA+GGG	0.577726	6.1:+11547841	MS.gene87985:CDS
TCTCTTTCGAATGGGTGGAT+CGG	0.581140	6.1:+11547664	None:intergenic
TGTTCATTGGCAATGGAACA+GGG	0.590457	6.1:-11548312	None:intergenic
CATGCATACGGGCGGTAGAG+CGG	0.599254	6.1:+11548018	MS.gene87985:CDS
ACTCGGTGTTAAACTATCGA+AGG	0.617292	6.1:+11547820	MS.gene87985:CDS
TGTCTTATTGTGAAGCTAAG+GGG	0.629341	6.1:+11548152	MS.gene87985:CDS
CGTACACACATAACATCGCT+CGG	0.629342	6.1:+11547872	MS.gene87985:CDS
GCAATTGTAACTGTTGTCAT+CGG	0.639808	6.1:-11547768	None:intergenic
CCTACTCTCAAGTCATCCAT+CGG	0.642889	6.1:+11547691	MS.gene87985:CDS
CATTCGTTGCGCACTCACGT+AGG	0.677333	6.1:+11547743	MS.gene87985:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAAAGGGACATAAAATA+TGG	+	chr6.1:11548179-11548198	MS.gene87985:CDS	20.0%
!!	TTAATCATATGTTTGTTCAT+TGG	-	chr6.1:11548328-11548347	None:intergenic	20.0%
!	AGAATGACACTATATAGATA+TGG	+	chr6.1:11548097-11548116	MS.gene87985:CDS	25.0%
!	ATGTGAAAAATTTAGTTGAG+AGG	+	chr6.1:11547930-11547949	MS.gene87985:CDS	25.0%
!	CTAAATCATTGAAAAATGCT+TGG	+	chr6.1:11548269-11548288	MS.gene87985:CDS	25.0%
!!!	CAAGCATTTTTCAATGATTT+AGG	-	chr6.1:11548271-11548290	None:intergenic	25.0%
	AACTTAAAATTCGGCATGTA+TGG	-	chr6.1:11547980-11547999	None:intergenic	30.0%
	ATATGTTTGTTCATTGGCAA+TGG	-	chr6.1:11548322-11548341	None:intergenic	30.0%
	ATCATTGAAAAATGCTTGGA+TGG	+	chr6.1:11548273-11548292	MS.gene87985:CDS	30.0%
	GAAGACGAAAAAGAAATACT+CGG	+	chr6.1:11547803-11547822	MS.gene87985:CDS	30.0%
	TCGAACGCTAACTTAAAATT+CGG	-	chr6.1:11547989-11548008	None:intergenic	30.0%
	TTAAGGTTAATACTGCTGTT+TGG	+	chr6.1:11548224-11548243	MS.gene87985:CDS	30.0%
!	AAAATATGGCAAATGGCTTT+TGG	+	chr6.1:11548193-11548212	MS.gene87985:CDS	30.0%
!	ACTTGAGTTTTTCCGAAATT+GGG	-	chr6.1:11547910-11547929	None:intergenic	30.0%
!	ATTGTCTTATTGTGAAGCTA+AGG	+	chr6.1:11548150-11548169	MS.gene87985:CDS	30.0%
!	CTATATAGTGTCATTCTTGA+AGG	-	chr6.1:11548094-11548113	None:intergenic	30.0%
!	TACTTGAGTTTTTCCGAAAT+TGG	-	chr6.1:11547911-11547930	None:intergenic	30.0%
!	TTGTCTTATTGTGAAGCTAA+GGG	+	chr6.1:11548151-11548170	MS.gene87985:CDS	30.0%
!!	TGAGCTTGATGATTTTCATA+TGG	+	chr6.1:11548066-11548085	MS.gene87985:CDS	30.0%
	ACACATCATCAAGTTCTGTT+TGG	+	chr6.1:11548122-11548141	MS.gene87985:CDS	35.0%
	GCAATTGTAACTGTTGTCAT+CGG	-	chr6.1:11547771-11547790	None:intergenic	35.0%
	GGGACATAAAATATGGCAAA+TGG	+	chr6.1:11548186-11548205	MS.gene87985:CDS	35.0%
!	CGATCAATTTTGCATGCATA+CGG	+	chr6.1:11548006-11548025	MS.gene87985:CDS	35.0%
!	GATCAATTTTGCATGCATAC+GGG	+	chr6.1:11548007-11548026	MS.gene87985:CDS	35.0%
!	TGTCTTATTGTGAAGCTAAG+GGG	+	chr6.1:11548152-11548171	MS.gene87985:CDS	35.0%
!!	GCATCAACTGATATTTTGAG+CGG	-	chr6.1:11547724-11547743	None:intergenic	35.0%
	AGCTAAGGGGAGAGTTAAAA+AGG	+	chr6.1:11548165-11548184	MS.gene87985:CDS	40.0%
	AGGATCTCATGAATGTTGCT+AGG	+	chr6.1:11547840-11547859	MS.gene87985:CDS	40.0%
	GCTAAGGGGAGAGTTAAAAA+GGG	+	chr6.1:11548166-11548185	MS.gene87985:CDS	40.0%
	GGATCTCATGAATGTTGCTA+GGG	+	chr6.1:11547841-11547860	MS.gene87985:CDS	40.0%
	TCCATTAGTCCTCCCAATTT+CGG	+	chr6.1:11547895-11547914	MS.gene87985:CDS	40.0%
	TGTTCATTGGCAATGGAACA+GGG	-	chr6.1:11548315-11548334	None:intergenic	40.0%
	TTGTTCATTGGCAATGGAAC+AGG	-	chr6.1:11548316-11548335	None:intergenic	40.0%
!	GGCTTTTGGATCTGGATTTA+AGG	+	chr6.1:11548207-11548226	MS.gene87985:CDS	40.0%
!	TGAGTTTTTCCGAAATTGGG+AGG	-	chr6.1:11547907-11547926	None:intergenic	40.0%
!!	ACTCGGTGTTAAACTATCGA+AGG	+	chr6.1:11547820-11547839	MS.gene87985:CDS	40.0%
!!	ACTGATATTTTGAGCGGCTA+CGG	-	chr6.1:11547718-11547737	None:intergenic	40.0%
	CCTACTCTCAAGTCATCCAT+CGG	+	chr6.1:11547691-11547710	MS.gene87985:CDS	45.0%
	CGATGGATGACTTGAGAGTA+GGG	-	chr6.1:11547693-11547712	None:intergenic	45.0%
	CGTACACACATAACATCGCT+CGG	+	chr6.1:11547872-11547891	MS.gene87985:CDS	45.0%
	TCCGAAATTGGGAGGACTAA+TGG	-	chr6.1:11547899-11547918	None:intergenic	45.0%
!	CAATTTTGCATGCATACGGG+CGG	+	chr6.1:11548010-11548029	MS.gene87985:CDS	45.0%
!	TGGCAAATGGCTTTTGGATC+TGG	+	chr6.1:11548199-11548218	MS.gene87985:CDS	45.0%
	CCGATGGATGACTTGAGAGT+AGG	-	chr6.1:11547694-11547713	None:intergenic	50.0%
	CATTCGTTGCGCACTCACGT+AGG	+	chr6.1:11547743-11547762	MS.gene87985:CDS	55.0%
	GCGGTAGAGCGGTTCTTGAT+CGG	+	chr6.1:11548029-11548048	MS.gene87985:CDS	55.0%
	CATGCATACGGGCGGTAGAG+CGG	+	chr6.1:11548018-11548037	MS.gene87985:CDS	60.0%
	TTTGAGCGGCTACGGTCCGA+TGG	-	chr6.1:11547710-11547729	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	11547674	11548354	11547674	ID=MS.gene87985
chr6.1	mRNA	11547674	11548354	11547674	ID=MS.gene87985.t1;Parent=MS.gene87985
chr6.1	exon	11547674	11548354	11547674	ID=MS.gene87985.t1.exon1;Parent=MS.gene87985.t1
chr6.1	CDS	11547674	11548354	11547674	ID=cds.MS.gene87985.t1;Parent=MS.gene87985.t1

Gene Sequence

>MS.gene87985

ATGGGTGGATCGGCAGCCCTACTCTCAAGTCATCCATCGGACCGTAGCCGCTCAAAATATCAGTTGATGCATTCGTTGCGCACTCACGTAGGCGCCGATGACAACAGTTACAATTGCGTCTTTCAAGAAGAAGACGAAAAAGAAATACTCGGTGTTAAACTATCGAAGGATCTCATGAATGTTGCTAGGGATGCACTTCGTACACACATAACATCGCTCGGTCCATTAGTCCTCCCAATTTCGGAAAAACTCAAGTATGTGAAAAATTTAGTTGAGAGGAAGATTTTGAAGAAGAAGATCGAACCATACATGCCGAATTTTAAGTTAGCGTTCGATCAATTTTGCATGCATACGGGCGGTAGAGCGGTTCTTGATCGGATGCAAAAGAGTCTTGAGCTTGATGATTTTCATATGGAGCCTTCAAGAATGACACTATATAGATATGGTAACACATCATCAAGTTCTGTTTGGTATGAATTGTCTTATTGTGAAGCTAAGGGGAGAGTTAAAAAGGGACATAAAATATGGCAAATGGCTTTTGGATCTGGATTTAAGGTTAATACTGCTGTTTGGTATGCTTTGAAGAATGTTGATCCTAAATCATTGAAAAATGCTTGGATGGATGAGATTGATGATTTCCCTGTTCCATTGCCAATGAACAAACATATGATTAAAGCTTAG

Protein sequence

>MS.gene87985.t1

MGGSAALLSSHPSDRSRSKYQLMHSLRTHVGADDNSYNCVFQEEDEKEILGVKLSKDLMNVARDALRTHITSLGPLVLPISEKLKYVKNLVERKILKKKIEPYMPNFKLAFDQFCMHTGGRAVLDRMQKSLELDDFHMEPSRMTLYRYGNTSSSSVWYELSYCEAKGRVKKGHKIWQMAFGSGFKVNTAVWYALKNVDPKSLKNAWMDEIDDFPVPLPMNKHMIKA