Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88646.t1 | XP_024633292.1 | 97.3 | 75 | 2 | 0 | 8 | 82 | 61 | 135 | 4.90E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88646.t1 | B7FJS9 | 97.3 | 75 | 2 | 0 | 8 | 82 | 61 | 135 | 3.5e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene88646 | 0.815285 | 1.00E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene88646 | 0.807724 | 4.40E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene88646 | 0.813192 | 2.90E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene88646 | 0.802929 | 4.44E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene88646 | 0.803232 | 3.85E-49 | -1.69E-46 |
| MS.gene053621 | MS.gene88646 | 0.800289 | 1.55E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene88646 | 0.815629 | 8.38E-52 | -1.69E-46 |
| MS.gene057869 | MS.gene88646 | 0.815034 | 1.14E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene88646 | 0.804368 | 2.23E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88646.t1 | MTR_2g021040 | 97.333 | 75 | 2 | 0 | 8 | 82 | 61 | 135 | 3.83e-48 | 154 |
| MS.gene88646.t1 | MTR_2g021040 | 97.333 | 75 | 2 | 0 | 8 | 82 | 61 | 135 | 2.00e-47 | 153 |
| MS.gene88646.t1 | MTR_2g021040 | 97.333 | 75 | 2 | 0 | 8 | 82 | 61 | 135 | 2.34e-47 | 153 |
| MS.gene88646.t1 | MTR_0003s0650 | 75.641 | 78 | 16 | 3 | 6 | 81 | 57 | 133 | 1.28e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88646.t1 | AT5G18940 | 67.568 | 74 | 24 | 0 | 8 | 81 | 61 | 134 | 9.05e-30 | 107 |
| MS.gene88646.t1 | AT5G18940 | 67.568 | 74 | 24 | 0 | 8 | 81 | 61 | 134 | 9.13e-30 | 107 |
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGAATTGCAGGATATATA+TGG | 0.358956 | 8.4:-6528730 | MS.gene88646:CDS |
| ATTCACAAGTTGCCTATACT+TGG | 0.363293 | 8.4:-6528631 | MS.gene88646:CDS |
| TGTGAAACCTGATCTAAATT+CGG | 0.430120 | 8.4:+6528697 | None:intergenic |
| ATCAGAACCGAATTTAGATC+AGG | 0.437545 | 8.4:-6528704 | MS.gene88646:CDS |
| GACGATATTGTTGAAACAAA+AGG | 0.442977 | 8.4:-6528491 | MS.gene88646:CDS |
| GTATTCTACACAGCAATCAT+TGG | 0.448420 | 8.4:+6528462 | None:intergenic |
| CTTACTTCCCATCCAAGTAT+AGG | 0.450135 | 8.4:+6528619 | None:intergenic |
| TACTAGACTTTCTTGTTGCA+TGG | 0.494982 | 8.4:-6528420 | None:intergenic |
| ACAAGTTGCCTATACTTGGA+TGG | 0.498032 | 8.4:-6528627 | MS.gene88646:CDS |
| GAAAGGATTTAGTCCACTGT+TGG | 0.511448 | 8.4:-6528513 | MS.gene88646:CDS |
| CAAGTTGCCTATACTTGGAT+GGG | 0.523597 | 8.4:-6528626 | MS.gene88646:CDS |
| AGATCAGGTTTCACAGCTTG+TGG | 0.526178 | 8.4:-6528689 | MS.gene88646:CDS |
| TCAGGTTTCACAGCTTGTGG+AGG | 0.621980 | 8.4:-6528686 | MS.gene88646:CDS |
| GCTTGTGGAGGAAATCTGCA+AGG | 0.666944 | 8.4:-6528674 | MS.gene88646:CDS |
| TCAACAATATCGTCCAACAG+TGG | 0.704888 | 8.4:+6528500 | None:intergenic |
| TGTGGAGGAAATCTGCAAGG+AGG | 0.743747 | 8.4:-6528671 | MS.gene88646:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTAGAATTGCAGGATATATA+TGG | - | chr8.4:6528421-6528440 | MS.gene88646:CDS | 30.0% | |
| GACGATATTGTTGAAACAAA+AGG | - | chr8.4:6528660-6528679 | MS.gene88646:CDS | 30.0% | |
| TGTGAAACCTGATCTAAATT+CGG | + | chr8.4:6528457-6528476 | None:intergenic | 30.0% | |
| !! | AATTTTGATACAGGCTAGAA+AGG | - | chr8.4:6528621-6528640 | MS.gene88646:intron | 30.0% |
| ATCAGAACCGAATTTAGATC+AGG | - | chr8.4:6528447-6528466 | MS.gene88646:CDS | 35.0% | |
| ATTCACAAGTTGCCTATACT+TGG | - | chr8.4:6528520-6528539 | MS.gene88646:CDS | 35.0% | |
| GTATTCTACACAGCAATCAT+TGG | + | chr8.4:6528692-6528711 | None:intergenic | 35.0% | |
| ACAAGTTGCCTATACTTGGA+TGG | - | chr8.4:6528524-6528543 | MS.gene88646:intron | 40.0% | |
| CAAGTTGCCTATACTTGGAT+GGG | - | chr8.4:6528525-6528544 | MS.gene88646:intron | 40.0% | |
| CTTACTTCCCATCCAAGTAT+AGG | + | chr8.4:6528535-6528554 | None:intergenic | 40.0% | |
| GAAAGGATTTAGTCCACTGT+TGG | - | chr8.4:6528638-6528657 | MS.gene88646:CDS | 40.0% | |
| TCAACAATATCGTCCAACAG+TGG | + | chr8.4:6528654-6528673 | None:intergenic | 40.0% | |
| ! | ATGCATGCAGTTGACTTAAC+TGG | - | chr8.4:6528578-6528597 | MS.gene88646:intron | 40.0% |
| !! | GGGTGCATGAATTTTGATAC+AGG | - | chr8.4:6528612-6528631 | MS.gene88646:intron | 40.0% |
| ACTTAACTGGTTGCTCATCG+TGG | - | chr8.4:6528591-6528610 | MS.gene88646:intron | 45.0% | |
| AGATCAGGTTTCACAGCTTG+TGG | - | chr8.4:6528462-6528481 | MS.gene88646:CDS | 45.0% | |
| CTTAACTGGTTGCTCATCGT+GGG | - | chr8.4:6528592-6528611 | MS.gene88646:intron | 45.0% | |
| GCTTGTGGAGGAAATCTGCA+AGG | - | chr8.4:6528477-6528496 | MS.gene88646:CDS | 50.0% | |
| TCAGGTTTCACAGCTTGTGG+AGG | - | chr8.4:6528465-6528484 | MS.gene88646:CDS | 50.0% | |
| TGTGGAGGAAATCTGCAAGG+AGG | - | chr8.4:6528480-6528499 | MS.gene88646:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6528421 | 6528752 | 6528421 | ID=MS.gene88646 |
| chr8.4 | mRNA | 6528421 | 6528752 | 6528421 | ID=MS.gene88646.t1;Parent=MS.gene88646 |
| chr8.4 | exon | 6528624 | 6528752 | 6528624 | ID=MS.gene88646.t1.exon1;Parent=MS.gene88646.t1 |
| chr8.4 | CDS | 6528624 | 6528752 | 6528624 | ID=cds.MS.gene88646.t1;Parent=MS.gene88646.t1 |
| chr8.4 | exon | 6528421 | 6528539 | 6528421 | ID=MS.gene88646.t1.exon2;Parent=MS.gene88646.t1 |
| chr8.4 | CDS | 6528421 | 6528539 | 6528421 | ID=cds.MS.gene88646.t1;Parent=MS.gene88646.t1 |
| Gene Sequence |
| Protein sequence |
