Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88663.t1 | XP_003610152.2 | 95.9 | 266 | 11 | 0 | 1 | 266 | 10 | 275 | 7.90E-150 | 539.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88663.t1 | Q38910 | 71.5 | 281 | 62 | 2 | 2 | 266 | 8 | 286 | 9.8e-117 | 421.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88663.t1 | G7JD11 | 95.9 | 266 | 11 | 0 | 1 | 266 | 10 | 275 | 5.7e-150 | 539.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052083 | MS.gene88663 | 0.818579 | 1.82E-52 | -1.69E-46 |
| MS.gene055977 | MS.gene88663 | 0.844352 | 7.98E-59 | -1.69E-46 |
| MS.gene056571 | MS.gene88663 | 0.817013 | 4.11E-52 | -1.69E-46 |
| MS.gene059855 | MS.gene88663 | 0.802642 | 5.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88663.t1 | MTR_4g128570 | 95.865 | 266 | 11 | 0 | 1 | 266 | 10 | 275 | 0.0 | 539 |
| MS.gene88663.t1 | MTR_7g093530 | 79.422 | 277 | 37 | 2 | 1 | 263 | 9 | 279 | 1.00e-163 | 455 |
| MS.gene88663.t1 | MTR_4g128410 | 78.700 | 277 | 39 | 2 | 1 | 263 | 10 | 280 | 3.39e-161 | 449 |
| MS.gene88663.t1 | MTR_4g128580 | 80.524 | 267 | 34 | 1 | 18 | 266 | 22 | 288 | 1.26e-157 | 440 |
| MS.gene88663.t1 | MTR_4g128400 | 69.818 | 275 | 68 | 2 | 5 | 265 | 12 | 285 | 8.98e-145 | 407 |
| MS.gene88663.t1 | MTR_4g128590 | 69.930 | 286 | 50 | 3 | 1 | 266 | 9 | 278 | 4.08e-143 | 402 |
| MS.gene88663.t1 | MTR_4g126920 | 71.536 | 267 | 61 | 1 | 13 | 264 | 16 | 282 | 3.43e-142 | 400 |
| MS.gene88663.t1 | MTR_2g038860 | 68.198 | 283 | 71 | 1 | 3 | 266 | 7 | 289 | 1.54e-141 | 399 |
| MS.gene88663.t1 | MTR_2g038705 | 68.198 | 283 | 71 | 1 | 3 | 266 | 7 | 289 | 1.54e-141 | 399 |
| MS.gene88663.t1 | MTR_8g081040 | 68.266 | 271 | 77 | 1 | 3 | 264 | 12 | 282 | 2.18e-138 | 391 |
| MS.gene88663.t1 | MTR_3g084620 | 69.630 | 270 | 73 | 2 | 2 | 263 | 9 | 277 | 2.88e-137 | 388 |
| MS.gene88663.t1 | MTR_3g084630 | 69.888 | 269 | 72 | 2 | 3 | 263 | 10 | 277 | 4.57e-137 | 387 |
| MS.gene88663.t1 | MTR_8g102220 | 65.918 | 267 | 82 | 3 | 2 | 266 | 10 | 269 | 1.08e-125 | 358 |
| MS.gene88663.t1 | MTR_5g091730 | 51.481 | 270 | 122 | 1 | 3 | 263 | 9 | 278 | 4.95e-100 | 294 |
| MS.gene88663.t1 | MTR_8g102910 | 51.957 | 281 | 113 | 4 | 1 | 263 | 3 | 279 | 2.99e-99 | 291 |
| MS.gene88663.t1 | MTR_3g089540 | 52.692 | 260 | 112 | 1 | 15 | 263 | 23 | 282 | 2.63e-98 | 290 |
| MS.gene88663.t1 | MTR_6g088320 | 50.538 | 279 | 118 | 3 | 3 | 263 | 7 | 283 | 6.64e-97 | 286 |
| MS.gene88663.t1 | MTR_6g033085 | 50.699 | 286 | 117 | 4 | 2 | 264 | 4 | 288 | 1.98e-96 | 285 |
| MS.gene88663.t1 | MTR_6g033055 | 51.786 | 280 | 116 | 4 | 3 | 264 | 10 | 288 | 3.06e-95 | 282 |
| MS.gene88663.t1 | MTR_7g084770 | 54.086 | 257 | 102 | 3 | 22 | 263 | 32 | 287 | 8.81e-95 | 281 |
| MS.gene88663.t1 | MTR_7g084760 | 51.799 | 278 | 116 | 4 | 3 | 263 | 11 | 287 | 1.50e-94 | 280 |
| MS.gene88663.t1 | MTR_7g084750 | 51.439 | 278 | 117 | 4 | 3 | 263 | 11 | 287 | 8.60e-94 | 278 |
| MS.gene88663.t1 | MTR_2g089140 | 48.084 | 287 | 123 | 5 | 1 | 264 | 4 | 287 | 3.25e-93 | 276 |
| MS.gene88663.t1 | MTR_4g097640 | 48.375 | 277 | 126 | 6 | 3 | 263 | 12 | 287 | 1.13e-90 | 270 |
| MS.gene88663.t1 | MTR_4g097670 | 48.375 | 277 | 126 | 6 | 3 | 263 | 12 | 287 | 1.13e-90 | 270 |
| MS.gene88663.t1 | MTR_4g097700 | 48.921 | 278 | 124 | 6 | 1 | 263 | 50 | 324 | 4.51e-90 | 270 |
| MS.gene88663.t1 | MTR_4g045853 | 47.887 | 284 | 120 | 6 | 4 | 264 | 11 | 289 | 4.88e-90 | 269 |
| MS.gene88663.t1 | MTR_1g105900 | 48.352 | 273 | 122 | 3 | 8 | 264 | 23 | 292 | 2.88e-89 | 267 |
| MS.gene88663.t1 | MTR_1g105495 | 45.862 | 290 | 129 | 5 | 4 | 266 | 9 | 297 | 3.35e-83 | 251 |
| MS.gene88663.t1 | MTR_2g089140 | 51.556 | 225 | 85 | 4 | 63 | 264 | 1 | 224 | 7.85e-81 | 243 |
| MS.gene88663.t1 | MTR_8g013055 | 45.914 | 257 | 122 | 4 | 22 | 263 | 31 | 285 | 4.33e-79 | 241 |
| MS.gene88663.t1 | MTR_7g056090 | 44.361 | 266 | 127 | 7 | 3 | 251 | 12 | 273 | 3.76e-69 | 215 |
| MS.gene88663.t1 | MTR_7g102260 | 40.000 | 270 | 137 | 7 | 15 | 265 | 30 | 293 | 1.92e-60 | 193 |
| MS.gene88663.t1 | MTR_2g049030 | 41.085 | 258 | 128 | 8 | 22 | 261 | 30 | 281 | 3.42e-59 | 189 |
| MS.gene88663.t1 | MTR_1g063020 | 40.840 | 262 | 130 | 9 | 21 | 263 | 39 | 294 | 2.80e-57 | 185 |
| MS.gene88663.t1 | MTR_2g049130 | 41.065 | 263 | 124 | 9 | 22 | 265 | 29 | 279 | 6.71e-57 | 184 |
| MS.gene88663.t1 | MTR_4g057450 | 41.985 | 262 | 127 | 8 | 21 | 263 | 49 | 304 | 1.58e-56 | 183 |
| MS.gene88663.t1 | MTR_2g095800 | 41.961 | 255 | 121 | 9 | 21 | 254 | 53 | 301 | 3.67e-55 | 180 |
| MS.gene88663.t1 | MTR_1g033420 | 36.260 | 262 | 146 | 5 | 22 | 264 | 33 | 292 | 1.49e-51 | 171 |
| MS.gene88663.t1 | MTR_5g029100 | 34.884 | 258 | 142 | 7 | 30 | 263 | 50 | 305 | 3.34e-47 | 160 |
| MS.gene88663.t1 | MTR_8g064180 | 34.981 | 263 | 144 | 6 | 26 | 263 | 50 | 310 | 4.05e-47 | 159 |
| MS.gene88663.t1 | MTR_8g090100 | 32.862 | 283 | 163 | 8 | 4 | 264 | 5 | 282 | 2.95e-44 | 152 |
| MS.gene88663.t1 | MTR_5g029100 | 35.043 | 234 | 126 | 7 | 54 | 263 | 43 | 274 | 1.24e-42 | 148 |
| MS.gene88663.t1 | MTR_8g479340 | 40.404 | 99 | 42 | 3 | 184 | 266 | 41 | 138 | 6.52e-16 | 73.2 |
| MS.gene88663.t1 | MTR_8g032640 | 31.609 | 174 | 111 | 4 | 44 | 215 | 33 | 200 | 2.28e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88663.t1 | AT4G25810 | 71.530 | 281 | 62 | 2 | 2 | 266 | 8 | 286 | 6.17e-151 | 423 |
| MS.gene88663.t1 | AT5G57560 | 68.905 | 283 | 66 | 2 | 1 | 266 | 7 | 284 | 5.29e-148 | 415 |
| MS.gene88663.t1 | AT4G30270 | 71.863 | 263 | 72 | 1 | 4 | 266 | 8 | 268 | 6.31e-147 | 412 |
| MS.gene88663.t1 | AT5G57550 | 68.345 | 278 | 72 | 2 | 2 | 263 | 5 | 282 | 2.43e-143 | 404 |
| MS.gene88663.t1 | AT5G57530 | 68.846 | 260 | 67 | 1 | 18 | 263 | 23 | 282 | 4.56e-134 | 380 |
| MS.gene88663.t1 | AT5G57540 | 68.340 | 259 | 68 | 1 | 19 | 263 | 23 | 281 | 4.95e-133 | 377 |
| MS.gene88663.t1 | AT4G25820 | 66.171 | 269 | 77 | 1 | 9 | 263 | 17 | 285 | 8.15e-133 | 377 |
| MS.gene88663.t1 | AT5G48070 | 61.029 | 272 | 95 | 2 | 4 | 264 | 11 | 282 | 2.09e-129 | 368 |
| MS.gene88663.t1 | AT4G14130 | 63.214 | 280 | 87 | 2 | 2 | 266 | 9 | 287 | 7.37e-129 | 367 |
| MS.gene88663.t1 | AT2G18800 | 59.932 | 292 | 90 | 3 | 1 | 265 | 7 | 298 | 8.66e-129 | 367 |
| MS.gene88663.t1 | AT3G23730 | 63.251 | 283 | 85 | 3 | 2 | 266 | 8 | 289 | 1.90e-128 | 366 |
| MS.gene88663.t1 | AT4G30290 | 62.868 | 272 | 90 | 2 | 4 | 264 | 6 | 277 | 1.15e-123 | 353 |
| MS.gene88663.t1 | AT4G30280 | 61.029 | 272 | 95 | 2 | 4 | 264 | 11 | 282 | 1.15e-121 | 348 |
| MS.gene88663.t1 | AT1G65310 | 63.424 | 257 | 83 | 2 | 19 | 264 | 48 | 304 | 9.86e-120 | 344 |
| MS.gene88663.t1 | AT1G65310 | 63.424 | 257 | 83 | 2 | 19 | 264 | 26 | 282 | 1.35e-119 | 343 |
| MS.gene88663.t1 | AT4G28850 | 50.178 | 281 | 119 | 3 | 3 | 264 | 10 | 288 | 8.21e-98 | 288 |
| MS.gene88663.t1 | AT5G13870 | 53.261 | 276 | 113 | 3 | 3 | 263 | 13 | 287 | 9.34e-96 | 283 |
| MS.gene88663.t1 | AT5G13870 | 53.261 | 276 | 113 | 3 | 3 | 263 | 13 | 287 | 9.34e-96 | 283 |
| MS.gene88663.t1 | AT5G13870 | 53.261 | 276 | 113 | 3 | 3 | 263 | 13 | 287 | 9.34e-96 | 283 |
| MS.gene88663.t1 | AT5G65730 | 45.714 | 280 | 135 | 5 | 1 | 265 | 15 | 292 | 1.26e-91 | 273 |
| MS.gene88663.t1 | AT2G06850 | 53.307 | 257 | 104 | 3 | 22 | 263 | 35 | 290 | 5.91e-91 | 271 |
| MS.gene88663.t1 | AT4G37800 | 47.842 | 278 | 127 | 5 | 5 | 265 | 15 | 291 | 1.25e-88 | 265 |
| MS.gene88663.t1 | AT4G03210 | 46.099 | 282 | 132 | 4 | 1 | 263 | 4 | 284 | 2.37e-88 | 264 |
| MS.gene88663.t1 | AT2G06850 | 55.507 | 227 | 85 | 3 | 52 | 263 | 5 | 230 | 9.14e-86 | 256 |
| MS.gene88663.t1 | AT2G14620 | 46.786 | 280 | 131 | 4 | 2 | 264 | 17 | 295 | 1.24e-85 | 258 |
| MS.gene88663.t1 | AT1G11545 | 46.316 | 285 | 131 | 5 | 3 | 266 | 19 | 302 | 3.03e-85 | 257 |
| MS.gene88663.t1 | AT4G03210 | 51.899 | 237 | 93 | 4 | 44 | 263 | 24 | 256 | 2.58e-84 | 253 |
| MS.gene88663.t1 | AT4G13090 | 46.388 | 263 | 122 | 5 | 18 | 263 | 28 | 288 | 9.58e-78 | 237 |
| MS.gene88663.t1 | AT2G14620 | 50.655 | 229 | 95 | 4 | 53 | 264 | 18 | 245 | 3.55e-76 | 232 |
| MS.gene88663.t1 | AT3G25050 | 45.174 | 259 | 121 | 5 | 22 | 263 | 35 | 289 | 3.40e-75 | 231 |
| MS.gene88663.t1 | AT4G13080 | 45.703 | 256 | 122 | 5 | 22 | 263 | 39 | 291 | 2.33e-74 | 229 |
| MS.gene88663.t1 | AT2G14620 | 51.364 | 220 | 89 | 4 | 62 | 264 | 16 | 234 | 3.20e-74 | 226 |
| MS.gene88663.t1 | AT2G36870 | 41.221 | 262 | 129 | 7 | 21 | 263 | 42 | 297 | 3.69e-58 | 187 |
| MS.gene88663.t1 | AT3G44990 | 40.996 | 261 | 129 | 8 | 22 | 263 | 39 | 293 | 2.96e-56 | 182 |
| MS.gene88663.t1 | AT1G32170 | 33.566 | 286 | 166 | 6 | 1 | 264 | 11 | 294 | 2.38e-51 | 171 |
| MS.gene88663.t1 | AT2G01850 | 32.993 | 294 | 166 | 6 | 1 | 265 | 1 | 292 | 5.18e-51 | 170 |
| MS.gene88663.t1 | AT1G14720 | 32.993 | 294 | 166 | 6 | 1 | 265 | 1 | 292 | 5.86e-51 | 170 |
| MS.gene88663.t1 | AT1G10550 | 35.769 | 260 | 136 | 6 | 33 | 263 | 52 | 309 | 4.37e-48 | 162 |
| MS.gene88663.t1 | AT3G48580 | 35.686 | 255 | 134 | 8 | 21 | 263 | 37 | 273 | 1.71e-44 | 152 |
| MS.gene88663.t1 | AT4G18990 | 33.582 | 268 | 143 | 6 | 30 | 264 | 48 | 313 | 1.07e-42 | 149 |
| MS.gene88663.t1 | AT3G48580 | 34.821 | 224 | 121 | 6 | 52 | 263 | 4 | 214 | 3.71e-39 | 136 |
| MS.gene88663.t1 | AT4G18990 | 34.500 | 200 | 99 | 5 | 95 | 264 | 42 | 239 | 1.23e-34 | 126 |
| MS.gene88663.t1 | AT2G36870 | 45.324 | 139 | 66 | 3 | 21 | 151 | 42 | 178 | 4.85e-33 | 119 |
| MS.gene88663.t1 | AT3G48580 | 30.588 | 255 | 127 | 9 | 21 | 263 | 37 | 253 | 1.33e-27 | 107 |
Find 61 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGACAAAGCTTCTGGTTC+TGG | 0.171186 | 8.1:-4415736 | MS.gene88663:CDS |
| ATCCTCATTGGTTGATTCTT+TGG | 0.185511 | 8.1:+4415069 | None:intergenic |
| ATGTTCTTGAATTCTCTTAT+TGG | 0.197118 | 8.1:+4415114 | None:intergenic |
| CTTTCTCTTGACAAAGCTTC+TGG | 0.235031 | 8.1:-4415742 | MS.gene88663:CDS |
| TTCACACCTACTCCATTCTT+TGG | 0.237903 | 8.1:-4415341 | MS.gene88663:CDS |
| TGAAATTGACTTTGAATTCT+TGG | 0.248522 | 8.1:-4415469 | MS.gene88663:CDS |
| TGTCATCAAAAGGATCAAAT+TGG | 0.258251 | 8.1:-4415494 | MS.gene88663:CDS |
| CTCCATTCACTGCATCTTAT+AGG | 0.269112 | 8.1:-4414975 | MS.gene88663:CDS |
| GAAATTGACTTTGAATTCTT+GGG | 0.327454 | 8.1:-4415468 | MS.gene88663:CDS |
| GCCAAAGAGATATTCATTCT+TGG | 0.330231 | 8.1:+4415705 | None:intergenic |
| GACTATCTTCCTATCTCTAT+TGG | 0.341863 | 8.1:-4415875 | MS.gene88663:CDS |
| GAGAGCAACAATTCCATCTA+TGG | 0.342180 | 8.1:-4415383 | MS.gene88663:CDS |
| TGAGGATATACTCAAGTCTA+TGG | 0.355078 | 8.1:-4415053 | MS.gene88663:CDS |
| TCCAAGAATGAATATCTCTT+TGG | 0.372292 | 8.1:-4415706 | MS.gene88663:CDS |
| TAGGATGCAGTGCACTCTAT+TGG | 0.390519 | 8.1:+4414808 | None:intergenic |
| CAGTTGGATCAAACCATAGA+TGG | 0.393171 | 8.1:+4415370 | None:intergenic |
| CCCATGTTATATCAAAGTTC+TGG | 0.398944 | 8.1:+4415809 | None:intergenic |
| TGCACTCTATTGGAAGGCCT+TGG | 0.405324 | 8.1:+4414818 | None:intergenic |
| GCACTCTATTGGAAGGCCTT+GGG | 0.408314 | 8.1:+4414819 | None:intergenic |
| AGAGATATTCATTCTTGGAT+TGG | 0.408705 | 8.1:+4415710 | None:intergenic |
| ACTTGTTGAATCCAACTCTT+GGG | 0.425232 | 8.1:+4414912 | None:intergenic |
| AGACTTGAGTATATCCTCAT+TGG | 0.432038 | 8.1:+4415057 | None:intergenic |
| GTCTATGGAATGCTGATGAT+TGG | 0.434923 | 8.1:-4415038 | MS.gene88663:CDS |
| GACTTGTTGAATCCAACTCT+TGG | 0.435704 | 8.1:+4414911 | None:intergenic |
| GTCATCAAAAGGATCAAATT+GGG | 0.451206 | 8.1:-4415493 | MS.gene88663:CDS |
| ACGGTGCCTGCAGAGTTTCC+AGG | 0.472156 | 8.1:+4415655 | None:intergenic |
| TGGCAAATGCCAATAGAGAT+AGG | 0.472883 | 8.1:+4415866 | None:intergenic |
| ATGCAGCTCAAGCTTGTACC+TGG | 0.480284 | 8.1:-4415673 | MS.gene88663:CDS |
| GGCATGCAGTTGTCATCAAA+AGG | 0.488525 | 8.1:-4415504 | MS.gene88663:intron |
| GAATTCTTGGGGAATGTAAG+TGG | 0.491509 | 8.1:-4415456 | MS.gene88663:CDS |
| TTCAAGAACATGGAATCAAA+AGG | 0.493604 | 8.1:-4415102 | MS.gene88663:CDS |
| TTCCTATAAGATGCAGTGAA+TGG | 0.501342 | 8.1:+4414973 | None:intergenic |
| GCGGTCTTGTCAAGACAGAT+TGG | 0.512024 | 8.1:-4415005 | MS.gene88663:CDS |
| TTCATTACAACATATGCAGC+AGG | 0.518113 | 8.1:-4415841 | MS.gene88663:CDS |
| AATGTGTTTAGTCAAGGAAA+AGG | 0.525149 | 8.1:-4415411 | MS.gene88663:CDS |
| TCTATGGAATGCTGATGATT+GGG | 0.538637 | 8.1:-4415037 | MS.gene88663:CDS |
| CACTCTATTGGAAGGCCTTG+GGG | 0.539602 | 8.1:+4414820 | None:intergenic |
| CTTGTACCTGGAAACTCTGC+AGG | 0.539694 | 8.1:-4415661 | MS.gene88663:CDS |
| GCGTTGAGGATTCCAAAGAA+TGG | 0.542490 | 8.1:+4415329 | None:intergenic |
| CACACCAATGTGTTTAGTCA+AGG | 0.543031 | 8.1:-4415417 | MS.gene88663:CDS |
| ACCAGAACTTTGATATAACA+TGG | 0.548519 | 8.1:-4415810 | MS.gene88663:CDS |
| AAATTGACTTTGAATTCTTG+GGG | 0.549232 | 8.1:-4415467 | MS.gene88663:CDS |
| ACATTGGTGTGAAGAGTGTA+TGG | 0.556406 | 8.1:+4415429 | None:intergenic |
| TTTGATATAACATGGGGAGA+TGG | 0.561650 | 8.1:-4415802 | MS.gene88663:CDS |
| AAACTACTTCATCTAATGCA+TGG | 0.572457 | 8.1:-4414939 | MS.gene88663:CDS |
| AGGATTCCAAAGAATGGAGT+AGG | 0.588075 | 8.1:+4415335 | None:intergenic |
| TGCATATGTTGTAATGAAAG+TGG | 0.604062 | 8.1:+4415846 | None:intergenic |
| TAGGTGTGAAAATCAGCAGT+TGG | 0.604383 | 8.1:+4415354 | None:intergenic |
| ATGCAGTGCACTCTATTGGA+AGG | 0.605313 | 8.1:+4414812 | None:intergenic |
| CCAGAACTTTGATATAACAT+GGG | 0.609957 | 8.1:-4415809 | MS.gene88663:CDS |
| AATAAGAGAATTCAAGAACA+TGG | 0.612760 | 8.1:-4415112 | MS.gene88663:CDS |
| GATGATTGGGCAACAAGAGG+CGG | 0.625561 | 8.1:-4415024 | MS.gene88663:CDS |
| GCTGATGATTGGGCAACAAG+AGG | 0.626727 | 8.1:-4415027 | MS.gene88663:CDS |
| GGTGCTTACACAATGCGTTG+AGG | 0.644219 | 8.1:+4415315 | None:intergenic |
| TTCCAAAGAATCAACCAATG+AGG | 0.645428 | 8.1:-4415071 | MS.gene88663:CDS |
| CGTTGTCAAGTATCTTAGCA+CGG | 0.649757 | 8.1:+4415779 | None:intergenic |
| AAGTCAACAAAGATTGAGTG+AGG | 0.654429 | 8.1:-4414893 | MS.gene88663:CDS |
| CAGAACTTTGATATAACATG+GGG | 0.667931 | 8.1:-4415808 | MS.gene88663:CDS |
| GACCTCAAAAGATTTCCCCA+AGG | 0.701224 | 8.1:-4414835 | MS.gene88663:CDS |
| ACTTACGTAATAAGCAGTGA+CGG | 0.708900 | 8.1:+4415636 | None:intergenic |
| CGTGCTAAGATACTTGACAA+CGG | 0.753379 | 8.1:-4415778 | MS.gene88663:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATTTAAAAATACTCAAT+TGG | - | chr8.1:4415482-4415501 | MS.gene88663:CDS | 10.0% |
| !!! | TGTTAATTATTTTTACTATA+TGG | - | chr8.1:4415159-4415178 | MS.gene88663:intron | 10.0% |
| !! | AATTCTATATCTATCAAACA+CGG | + | chr8.1:4415459-4415478 | None:intergenic | 20.0% |
| !! | TATGAGTATGATATTTGAAA+AGG | + | chr8.1:4415393-4415412 | None:intergenic | 20.0% |
| !! | TCATCAATAATCAATTCATT+GGG | + | chr8.1:4415509-4415528 | None:intergenic | 20.0% |
| !! | TTCATCAATAATCAATTCAT+TGG | + | chr8.1:4415510-4415529 | None:intergenic | 20.0% |
| ! | AATAAGAGAATTCAAGAACA+TGG | - | chr8.1:4415572-4415591 | MS.gene88663:intron | 25.0% |
| ! | ATGTTCTTGAATTCTCTTAT+TGG | + | chr8.1:4415573-4415592 | None:intergenic | 25.0% |
| ! | TATCATACTCATAAAAACAC+CGG | - | chr8.1:4415400-4415419 | MS.gene88663:CDS | 25.0% |
| !! | AAATTGACTTTGAATTCTTG+GGG | - | chr8.1:4415217-4415236 | MS.gene88663:intron | 25.0% |
| !! | GAAATTGACTTTGAATTCTT+GGG | - | chr8.1:4415216-4415235 | MS.gene88663:intron | 25.0% |
| !! | TGAAATTGACTTTGAATTCT+TGG | - | chr8.1:4415215-4415234 | MS.gene88663:intron | 25.0% |
| AAACTACTTCATCTAATGCA+TGG | - | chr8.1:4415745-4415764 | MS.gene88663:CDS | 30.0% | |
| AATGTGTTTAGTCAAGGAAA+AGG | - | chr8.1:4415273-4415292 | MS.gene88663:intron | 30.0% | |
| ACCAGAACTTTGATATAACA+TGG | - | chr8.1:4414874-4414893 | MS.gene88663:CDS | 30.0% | |
| AGAGATATTCATTCTTGGAT+TGG | + | chr8.1:4414977-4414996 | None:intergenic | 30.0% | |
| CAGAACTTTGATATAACATG+GGG | - | chr8.1:4414876-4414895 | MS.gene88663:CDS | 30.0% | |
| CCAGAACTTTGATATAACAT+GGG | - | chr8.1:4414875-4414894 | MS.gene88663:CDS | 30.0% | |
| GTCATCAAAAGGATCAAATT+GGG | - | chr8.1:4415191-4415210 | MS.gene88663:intron | 30.0% | |
| TCCAAGAATGAATATCTCTT+TGG | - | chr8.1:4414978-4414997 | MS.gene88663:CDS | 30.0% | |
| TGCATATGTTGTAATGAAAG+TGG | + | chr8.1:4414841-4414860 | None:intergenic | 30.0% | |
| TGTCATCAAAAGGATCAAAT+TGG | - | chr8.1:4415190-4415209 | MS.gene88663:intron | 30.0% | |
| TTCAAGAACATGGAATCAAA+AGG | - | chr8.1:4415582-4415601 | MS.gene88663:intron | 30.0% | |
| ! | AATTGAATGCAGTTTTTCTG+TGG | - | chr8.1:4415539-4415558 | MS.gene88663:intron | 30.0% |
| ! | TTTTCCTTGACTAAACACAT+TGG | + | chr8.1:4415274-4415293 | None:intergenic | 30.0% |
| AAGTCAACAAAGATTGAGTG+AGG | - | chr8.1:4415791-4415810 | MS.gene88663:CDS | 35.0% | |
| ACTTACGTAATAAGCAGTGA+CGG | + | chr8.1:4415051-4415070 | None:intergenic | 35.0% | |
| AGACTTGAGTATATCCTCAT+TGG | + | chr8.1:4415630-4415649 | None:intergenic | 35.0% | |
| CCCATGTTATATCAAAGTTC+TGG | + | chr8.1:4414878-4414897 | None:intergenic | 35.0% | |
| GACTATCTTCCTATCTCTAT+TGG | - | chr8.1:4414809-4414828 | MS.gene88663:CDS | 35.0% | |
| GCCAAAGAGATATTCATTCT+TGG | + | chr8.1:4414982-4415001 | None:intergenic | 35.0% | |
| TCTATGGAATGCTGATGATT+GGG | - | chr8.1:4415647-4415666 | MS.gene88663:CDS | 35.0% | |
| TGAGGATATACTCAAGTCTA+TGG | - | chr8.1:4415631-4415650 | MS.gene88663:intron | 35.0% | |
| TTCATTACAACATATGCAGC+AGG | - | chr8.1:4414843-4414862 | MS.gene88663:CDS | 35.0% | |
| TTCCAAAGAATCAACCAATG+AGG | - | chr8.1:4415613-4415632 | MS.gene88663:intron | 35.0% | |
| TTCCTATAAGATGCAGTGAA+TGG | + | chr8.1:4415714-4415733 | None:intergenic | 35.0% | |
| TTTGATATAACATGGGGAGA+TGG | - | chr8.1:4414882-4414901 | MS.gene88663:CDS | 35.0% | |
| ! | ACTTGTTGAATCCAACTCTT+GGG | + | chr8.1:4415775-4415794 | None:intergenic | 35.0% |
| ! | ATCCTCATTGGTTGATTCTT+TGG | + | chr8.1:4415618-4415637 | None:intergenic | 35.0% |
| AGGATTCCAAAGAATGGAGT+AGG | + | chr8.1:4415352-4415371 | None:intergenic | 40.0% | |
| ATTAGTGTCAGTATCGTGTC+CGG | + | chr8.1:4415422-4415441 | None:intergenic | 40.0% | |
| CACACCAATGTGTTTAGTCA+AGG | - | chr8.1:4415267-4415286 | MS.gene88663:intron | 40.0% | |
| CAGTTGGATCAAACCATAGA+TGG | + | chr8.1:4415317-4415336 | None:intergenic | 40.0% | |
| CGTGCTAAGATACTTGACAA+CGG | - | chr8.1:4414906-4414925 | MS.gene88663:CDS | 40.0% | |
| CGTTGTCAAGTATCTTAGCA+CGG | + | chr8.1:4414908-4414927 | None:intergenic | 40.0% | |
| CTCCATTCACTGCATCTTAT+AGG | - | chr8.1:4415709-4415728 | MS.gene88663:CDS | 40.0% | |
| CTTTCTCTTGACAAAGCTTC+TGG | - | chr8.1:4414942-4414961 | MS.gene88663:CDS | 40.0% | |
| GAATTCTTGGGGAATGTAAG+TGG | - | chr8.1:4415228-4415247 | MS.gene88663:intron | 40.0% | |
| GAGAGCAACAATTCCATCTA+TGG | - | chr8.1:4415301-4415320 | MS.gene88663:intron | 40.0% | |
| GTCTATGGAATGCTGATGAT+TGG | - | chr8.1:4415646-4415665 | MS.gene88663:CDS | 40.0% | |
| TAGGTGTGAAAATCAGCAGT+TGG | + | chr8.1:4415333-4415352 | None:intergenic | 40.0% | |
| TGGCAAATGCCAATAGAGAT+AGG | + | chr8.1:4414821-4414840 | None:intergenic | 40.0% | |
| TTCACACCTACTCCATTCTT+TGG | - | chr8.1:4415343-4415362 | MS.gene88663:CDS | 40.0% | |
| ! | ACATTGGTGTGAAGAGTGTA+TGG | + | chr8.1:4415258-4415277 | None:intergenic | 40.0% |
| ! | GAATGCAGTTTTTCTGTGGA+TGG | - | chr8.1:4415543-4415562 | MS.gene88663:intron | 40.0% |
| !! | GACTTGTTGAATCCAACTCT+TGG | + | chr8.1:4415776-4415795 | None:intergenic | 40.0% |
| ATGCAGTGCACTCTATTGGA+AGG | + | chr8.1:4415875-4415894 | None:intergenic | 45.0% | |
| GACCTCAAAAGATTTCCCCA+AGG | - | chr8.1:4415849-4415868 | MS.gene88663:CDS | 45.0% | |
| GCGTTGAGGATTCCAAAGAA+TGG | + | chr8.1:4415358-4415377 | None:intergenic | 45.0% | |
| GGCATGCAGTTGTCATCAAA+AGG | - | chr8.1:4415180-4415199 | MS.gene88663:intron | 45.0% | |
| TAGGATGCAGTGCACTCTAT+TGG | + | chr8.1:4415879-4415898 | None:intergenic | 45.0% | |
| ! | TGCATGGTTTTCCCAAGAGT+TGG | - | chr8.1:4415761-4415780 | MS.gene88663:CDS | 45.0% |
| !! | CTTGACAAAGCTTCTGGTTC+TGG | - | chr8.1:4414948-4414967 | MS.gene88663:CDS | 45.0% |
| ATGCAGCTCAAGCTTGTACC+TGG | - | chr8.1:4415011-4415030 | MS.gene88663:CDS | 50.0% | |
| CACTCTATTGGAAGGCCTTG+GGG | + | chr8.1:4415867-4415886 | None:intergenic | 50.0% | |
| CTTGTACCTGGAAACTCTGC+AGG | - | chr8.1:4415023-4415042 | MS.gene88663:CDS | 50.0% | |
| GATGATTGGGCAACAAGAGG+CGG | - | chr8.1:4415660-4415679 | MS.gene88663:CDS | 50.0% | |
| GCACTCTATTGGAAGGCCTT+GGG | + | chr8.1:4415868-4415887 | None:intergenic | 50.0% | |
| GCTGATGATTGGGCAACAAG+AGG | - | chr8.1:4415657-4415676 | MS.gene88663:CDS | 50.0% | |
| GGTGCTTACACAATGCGTTG+AGG | + | chr8.1:4415372-4415391 | None:intergenic | 50.0% | |
| TGCACTCTATTGGAAGGCCT+TGG | + | chr8.1:4415869-4415888 | None:intergenic | 50.0% | |
| ! | GCGGTCTTGTCAAGACAGAT+TGG | - | chr8.1:4415679-4415698 | MS.gene88663:CDS | 50.0% |
| ! | GGCCTTGGGGAAATCTTTTG+AGG | + | chr8.1:4415854-4415873 | None:intergenic | 50.0% |
| ACGGTGCCTGCAGAGTTTCC+AGG | + | chr8.1:4415032-4415051 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 4414807 | 4415899 | 4414807 | ID=MS.gene88663 |
| chr8.1 | mRNA | 4414807 | 4415899 | 4414807 | ID=MS.gene88663.t1;Parent=MS.gene88663 |
| chr8.1 | exon | 4415642 | 4415899 | 4415642 | ID=MS.gene88663.t1.exon1;Parent=MS.gene88663.t1 |
| chr8.1 | CDS | 4415642 | 4415899 | 4415642 | ID=cds.MS.gene88663.t1;Parent=MS.gene88663.t1 |
| chr8.1 | exon | 4415324 | 4415517 | 4415324 | ID=MS.gene88663.t1.exon2;Parent=MS.gene88663.t1 |
| chr8.1 | CDS | 4415324 | 4415517 | 4415324 | ID=cds.MS.gene88663.t1;Parent=MS.gene88663.t1 |
| chr8.1 | exon | 4414807 | 4415155 | 4414807 | ID=MS.gene88663.t1.exon3;Parent=MS.gene88663.t1 |
| chr8.1 | CDS | 4414807 | 4415155 | 4414807 | ID=cds.MS.gene88663.t1;Parent=MS.gene88663.t1 |
| Gene Sequence |
| Protein sequence |
